Sed0017967 (gene) Chayote v1

Overview
NameSed0017967
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG13: 8136943 .. 8143607 (-)
RNA-Seq ExpressionSed0017967
SyntenySed0017967
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGAATAGAGACACACAGGTGCAAGATTCCCTCATTTCTTCATAATTCATATTCCCATTTTTTATTGAAGCCTCTCATTCTCTCCAAATACTTATCTCTACACATTAATTCCCATTGCCAGCATCCATGACTATGAGCACCCTAGTGAAGCTGTTGTTACTCGTGGCGGTGATGCTGTCCTGCGCGGCAGCGTCTTCGGGCCGAAACTGGACTGTCGGGGGCAGCGGCCGGGGAATGAGAAAAGTTGAAGATGACTACTTGAAGTGGATAAGGCGGATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACTAAGAACAAGTTCAAGCCTTGTTTGACGCTCAAGGTTTCCAAGAATCCTAAGCCGGGCGGTTTCCGGTCGGTTCAGAAGGCTGTTAACTCTCTCCCGGTTCTCAACAGTTGCCGGGTTGTTATCCATGTCGGCGCCGGAGTCTACAGGTTAGTTTCGTTTTTACTCATTTACTTTAATGTTACGGTTCGTTTTGTATTAAAATTATAAATAGATTCAATTACAATTTAGTTCAAAAGCTTTCGGATATATATTTAATTGCTGCTACATTTTAAAATATGTTTAATTTTATATCTAATGGGGTTGTAAATTCTAAGTTGTGTCAACTAGTTTGTTTGTAGTGGGTGGGGAGTTCGTTTGTATGGCGACCTCATATTAATATTAATATTTTTATTTTAGAAAACAAATCATAAGTTAACTTTAAATAATCATTTGATTTTAAGTTTTCATTTTTAAAAATATAACCTATAAAATATTATTTTATTATTCCGACTTTTTTAGGGGATTTTGCATATATAACAAAAAATGGCAAAATATTTTCATCTATAGAATAAAATCCAAAATAATTTCATTTATAGCAGAAAATCACTTCTATCACTTGTTGAGTTCTATTAATGATAGAATTCAATCAATGATAGACTCATTCTATCACTGCTTAAATTTTTTATCAGTGATAGAAATCTAAATTGAGTTCTATCACTAGGTAAGTGATAGAAATAAATATGTTATAGATGAATTATTTTAAAATTTTTGCTGCAAATATAATACAAGAGGTAGAATGTGCTTCTGATGGTAAATTCTCACTTTTTTATGTCATCCATTTTACTCATACTTAAAAAAAACAAACAAACAACTCAAATTTTAGAAGAAAAAATAGGTAAAAATTTTATTTTAGTTTTTAAATTGAGAAAAAACTCAAATGATTCTATAAAAAAAATATAAAAAATTATTGATGAGATCAAATGAGTAAAAGAAAATTAGGAAAAAAATGAATATAACTTTAAAAAATAATAATAATAAATATTTTATTTTTTATTTTGAATTTTTTTAAAATTAGTTTTTTCTCTCTCAATTTCTTCTCCTATATTGTGTAACCTTAAAATAAATATTTAAATTCTGAATTATATTTAAAAAGAAAAATCCAAAATTATATATAAGATAATCTTCAATTTTTTTTTTCTTTTTGGATACAAACTAGGGGAGGAATTATTTAAAAAAAAAACTAGAGGAGGAAGGCTAAAGTGTACATAAAGATTTATTGCTCTCAAATAAATAAAAGTAAAAATATCGACTTTTAAGATTAAACAAAGAAACCAACCAGTCATCCATCTTTTTAAAATAATGTATATATAAATATTTGAAAGTAATCTTTCCATTCCAAACAATGAGAATGGAGGAATTAGAATCAAAAGTATGTAATGTGAATATTTATTATATGAACGAGAGAGAAATAAAAGGATGAATATGTTGGCCTTATTGTTTGTGTCTTTTTGTTGGGTTAAAAGGGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGTGAGGGAGCGGAGAAGACGATCATCGAATGGGGCGACACCGCGGACCATGTCGGAGACAACGGCCATCCCATGGGTACCTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCTAGGAACATCACTTTCAAGGTAACTCTTACCCAACATTTTTTTATTTTAAAATAAAATCCCTCCACTAATTTTTAGATTTAAATTCCATTCCATGGATTTTAATTTTTACAATTTAGTGAAAAACTAAGCCCCTTCCATAATTATTTGGTTTTTTATTTTTCATTTTTGAAATTTAAATTTATTTTTACTCAAGTTTCCTACCAATGTATTTGATTTTTCCTACAAATATTAATAAAATTTTGGAAGCGTTCCCAAATTTTAATTTGATTTCTTAAGATATAGGTAAGATGTAGATATTCAAATAAGAAAAAAAATGTATAGTAAAAAAAATTGTCATAGACTTAAATGTCAAAACCAAAAACTAAAAATAAAATGATTATCAGAGGGGGCTAAAGAATTGTTGGACATATTCATCTAATTCCACATTGAAAAGATGGAGAGTCTTCGATTATTTTTCTCACTAACAATTTATTTTGAGGTGAAACTCTATATTATTTAATATGATATTATAATTTTTTATAAATTTTAGTCCACAATTTTTATAAGAGATGTGAACTACTTTTATCGTTATCAATTAATATTAGATGAACCTATTTTATTTAACAGTAATGCATGGTTTGGGTTAAACTATTATTTTATGTTGGTTTCCGTACGTTTCATAGAATTTAAATCTATTAAAATTATTAACTAAATAATATTTTAAAATATTATTGGAGATTCTAGGGACCAAAATGAAAATAGAACTCAAAAGCAAAATAAGGATAATTTTTTCTAACCCAAATAATTTAAATGCTCTTTCTCATTCACTTTTTTTCTATCACTTAGTCACTATACTTGATGTTTTTTTTTTTAATTTAAAAAATATTTAATTTATCAAGTTTAAATTAACATATAGGTTAACAAGTTTATATTTTGATTTTATTTTTCCTTTTTAGAGATTCCCACAACTTTTTATTAGTATCTTTAAGTAAATTTTTTTTTCATTTTTAAAGGCAAGTTTTTATCTATTTTTGTCTTTTTTTAAATATATATTGGGGAAAAGATTTGCTTTAACATTTTACAATTATTCGAATGAGTTATGTTTCGATTCATAACCTTATATATTTTATTCAACCTAAATTTGTAGTCATCTTTTTTTTTTTACAAATATCCAGTAGAGATTGAACTTTTTAAAAAAAAAAAAAAATTAGAAATTGAACTTCTAAGGGGACTATGCATATTTTATGGCAGTTCAATACTCAATTTATTAATTAAATATACAAATTAAAAATGAGAAGGAATAAAGTTTGAATGTCGTATGTAAATGGCAATTTGCTTGTGACTCTCGATGACCAAACTGACATGTTTGCATTTTTTTTGTAATACAATGACCAAACTGACATGTTGTTCTTGGAATGATATGGACAACAATTAAAAATATCATAATCATCCAGATGAAATTCAAAATAATAATGTGTTTTTTCAATTATAATATATATAACTGTATATAAAACAATTTATACGGAATTGTTTTGTACTTTTGTTTTCTTTGTTTGTTGGACAGGGAATTAAATAAATATTTATTTAAATAAAGTTTCAATCTTTGTATTTTTTTATTTAATTTAATTAAATTTCCGTGCCGTAGAAACAGTTGGTTCAACTCCAACTATTGTATCTACTTTATATTTTGATCCTCCAAACTTCTAAAATTTCAAACTTAGCCCTTAACTTCTATTGAAATATAGATGTAAATCATACTTAGATCGCCCTATTCTCCAAACTTTAAATATTGTATTTGAGCTCTTCAAATTTTAATGTTCTATTTGCAATTTCAACTATTTAAGAAATTAGTACTAAAAAATCTATTTAAGAAATTATTTTACTACCCGATTAGAGACAAGGTTCAAATATTCAAAGATTAATAATATATATAAAAATAAAATGAATTATTTAAATTTATTTGACATTTTTTAAAATTTAGAACTCTAAACTTATAATTTAATCAAATATATTCTAGCTAGAAAAGAAACAAATTATATATCGGACTATACTTAAAGAATCTCGGTGTCACAGTCTAGAAACGGTTCAGTGGTACAGGTATAAGGGAACAAAACTCTGATTTCTCAATGTTAAAAAAAAACTATAATTAAATAAAAATCAAATATTATTAATAGTAAGGAATGAATGAAGTATATACAAACTTCAACGGCTCTTAAATAAAAGAGAATCAAATACTGATGGTAGGAATCAAACTATGACCATAATATTTAACTAAATTTTCATCAAACAATGAAATCATTTGACCAATGACCCTCTAAAATAATTGTATACGAAAAATTCGCAATTAGTCGAAACAATCATATATTGAATAGTTTGGAAGACCATTATATATAAACTTAAATGAATAATGTACATCAATGGGGAAGTAATCCTTTACGACATTCAAAATCGTGTCTTTTTTTCTTTTTCTTTTTTTGAGTACCGTGTCTTTTTTTTTTTTTTTAATTACAACAATTCAAAATCGTGTCAATTTAGTCATTATAATAAAAATGAATTTATTACTTTCTTTTGAATTTGTCCACCTAATAGATTATTGCCCTTATTAACATTCTAACTATATATTATTAAAAAATAAATTTGATGTGTTGATTTCAAGCAAGATTCTGGAATAAACACAATCAGCCTATATCACATATGTTGACTTCAGAGATTTATTAAAGATGGAAAATGTGTAAAATATCATTTTTGTCCTTGTTGCATTTTTAATTTTACATATTTATACATGTTCAATTTTAGTTTCAGTATTTTCAATAAATCCTAAAATTTGACCCTATCATTAGTATAAGATTAATTTTTTTTGGAATTAGTTAAATATTTATGATAATAATAATAGACTTTGATCCAAAAAACAATAAACACTAGTTTTTCTTTCCAAGAAAGTAGAAAATAAACAGAAACTAATTTTAAGTTGGTGACTAATTTCTAAAGCCTCACAAAACCATTTGATTTTTTATTTTTTATTTTTTTTCAAAATGTTACAACAATCTTCATCTTCTTTATAATTTTTTTTTTCTTCGAATTCTTTGGGGTATTCCAGCTAAAACAGAAACTAAGTTTTAAAAAACTTTTATAGAAATGGCAACACACAAATGCACAGCTAAAATCAATGTCGTCTTATTTGAGCAACAGAACAAGGCTCGGTTGCCGCCCTCGGGGGCGCTGGGGAAGCAAGCGGTAGCGTTCAGGATAACGGGGGACGCGGCGGCGTTCATAAGCTGCAAGTTCATGGGAGCGCAGGACACGCTGTACGACCACATGGGGCGCCACTACTTCAAGGACTGCTACATTGAGGGCTCGGTCGACTTCGTGTTCGGCGACGGCCTCTCCATTTACGACTCGTGCCACTTGCACGCCATCACCGACACCTACGGGGCTCTCACTGCCCAGAAGAGGGACAGCCTGCTGGAGGAGACCGGGTTCTCGTTTCTGCACTGTAAGGTTTCCGGGTCGGGTGCCTTGTTTTTGGGTCGGGCGTGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAACCGTGAACTGTAAGTCCTTTTTCTTTCTTTATTATGTTTGTCTTATTTTATTTTTAAATTATACCTATAAGTGCTTTTTTTACCTACTTTTTTTAATATTTTCATTTTTTCAAAAAAAAATTCGAAGAAAAGTTTAGAAACTAGGTGAAAAACACTTAATTTTCAAAATAAAAAATTATGGCCCCATGTCATTTGGATAAATATTTTAAAAAATTTTAGCTTATTTTTATTTAAATTTTCTATAATATATTTTATCTTTCCTTAAAAAATATGTGAATACTAACTGAATTTCAAAACCCAAAACAAGCTAGCTAAATTCTTTTCTCTCTCATGATTTATTTTTACCCCATGTATTTGAATATAGTTTTCTTTCTTTAATGTTTTGTTTTACCAAAAGTTTCTAAAATTTTAGTCCATAGTTTCAAAAATGGAACTTTCTTTTTTTAGTTTCCCAGATTATAATTTTAAAAACCGGTCTTATATTTAAATAGAAATTAGAAACAAAAAAAAAACAAAAGTGGTAGTAAATAAGCATAGATATAAAAGTAGATTTCTTTAGATAAATTTATTGGATTTATATTTATATGGAGGAGAAGAGACAAAAATGTAAAAAAAAAAGAAATGATGAGGTTTGTTTGGATTGTAGGACTGTATTTTATGGGCAATACAGATGCTCTGGACCGGGCGCGGATTATGGCGGTCGAGTTCCATGGTCCAGAGAACTCACCGAGTCAGAAGCCAACCCGTTCCTCTCTCTTGATTTCATTAATGCAAATCAATGGCTTCCAAAGCACACTCATCTCACCATTAAACCACATTAACAACTTAATATAATTTTCAATTTTCTTTTCGTGAGCTTATTTCAACTATTATGAGTAATTTTTTTTTTTAAATTTTGAATTGAATATAAGCTTGCTTGTAATAAAAGGAAAACTTTATTATATTGATGTAAGTTGTGAGTAATTATTTTATTGCTTGTGCTCGTGTCTTTTAAGATTTATTTATCCACGAGGTTTTCATTTTCATTTTTGAATTGAATTTAAGCTTGTTTGTAATTTAGTGTTGAAAGAAGTTGC

mRNA sequence

TATGAATAGAGACACACAGGTGCAAGATTCCCTCATTTCTTCATAATTCATATTCCCATTTTTTATTGAAGCCTCTCATTCTCTCCAAATACTTATCTCTACACATTAATTCCCATTGCCAGCATCCATGACTATGAGCACCCTAGTGAAGCTGTTGTTACTCGTGGCGGTGATGCTGTCCTGCGCGGCAGCGTCTTCGGGCCGAAACTGGACTGTCGGGGGCAGCGGCCGGGGAATGAGAAAAGTTGAAGATGACTACTTGAAGTGGATAAGGCGGATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACTAAGAACAAGTTCAAGCCTTGTTTGACGCTCAAGGTTTCCAAGAATCCTAAGCCGGGCGGTTTCCGGTCGGTTCAGAAGGCTGTTAACTCTCTCCCGGTTCTCAACAGTTGCCGGGTTGTTATCCATGTCGGCGCCGGAGTCTACAGGGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGTGAGGGAGCGGAGAAGACGATCATCGAATGGGGCGACACCGCGGACCATGTCGGAGACAACGGCCATCCCATGGGTACCTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCTAGGAACATCACTTTCAAGAACAAGGCTCGGTTGCCGCCCTCGGGGGCGCTGGGGAAGCAAGCGGTAGCGTTCAGGATAACGGGGGACGCGGCGGCGTTCATAAGCTGCAAGTTCATGGGAGCGCAGGACACGCTGTACGACCACATGGGGCGCCACTACTTCAAGGACTGCTACATTGAGGGCTCGGTCGACTTCGTGTTCGGCGACGGCCTCTCCATTTACGACTCGTGCCACTTGCACGCCATCACCGACACCTACGGGGCTCTCACTGCCCAGAAGAGGGACAGCCTGCTGGAGGAGACCGGGTTCTCGTTTCTGCACTGTAAGGTTTCCGGGTCGGGTGCCTTGTTTTTGGGTCGGGCGTGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAACCGTGAACTGACTGTATTTTATGGGCAATACAGATGCTCTGGACCGGGCGCGGATTATGGCGGTCGAGTTCCATGGTCCAGAGAACTCACCGAGTCAGAAGCCAACCCGTTCCTCTCTCTTGATTTCATTAATGCAAATCAATGGCTTCCAAAGCACACTCATCTCACCATTAAACCACATTAACAACTTAATATAATTTTCAATTTTCTTTTCGTGAGCTTATTTCAACTATTATGAGTAATTTTTTTTTTTAAATTTTGAATTGAATATAAGCTTGCTTGTAATAAAAGGAAAACTTTATTATATTGATGTAAGTTGTGAGTAATTATTTTATTGCTTGTGCTCGTGTCTTTTAAGATTTATTTATCCACGAGGTTTTCATTTTCATTTTTGAATTGAATTTAAGCTTGTTTGTAATTTAGTGTTGAAAGAAGTTGC

Coding sequence (CDS)

ATGACTATGAGCACCCTAGTGAAGCTGTTGTTACTCGTGGCGGTGATGCTGTCCTGCGCGGCAGCGTCTTCGGGCCGAAACTGGACTGTCGGGGGCAGCGGCCGGGGAATGAGAAAAGTTGAAGATGACTACTTGAAGTGGATAAGGCGGATGGGTTCTTTCAAGCACTCGCTTTTTCAGAAGACTAAGAACAAGTTCAAGCCTTGTTTGACGCTCAAGGTTTCCAAGAATCCTAAGCCGGGCGGTTTCCGGTCGGTTCAGAAGGCTGTTAACTCTCTCCCGGTTCTCAACAGTTGCCGGGTTGTTATCCATGTCGGCGCCGGAGTCTACAGGGAGAAGGTTGAGATTCCGGCAACGATGAGTTACATATGGGTGGAGGGTGAGGGAGCGGAGAAGACGATCATCGAATGGGGCGACACCGCGGACCATGTCGGAGACAACGGCCATCCCATGGGTACCTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCTTTCTTCATTGCTAGGAACATCACTTTCAAGAACAAGGCTCGGTTGCCGCCCTCGGGGGCGCTGGGGAAGCAAGCGGTAGCGTTCAGGATAACGGGGGACGCGGCGGCGTTCATAAGCTGCAAGTTCATGGGAGCGCAGGACACGCTGTACGACCACATGGGGCGCCACTACTTCAAGGACTGCTACATTGAGGGCTCGGTCGACTTCGTGTTCGGCGACGGCCTCTCCATTTACGACTCGTGCCACTTGCACGCCATCACCGACACCTACGGGGCTCTCACTGCCCAGAAGAGGGACAGCCTGCTGGAGGAGACCGGGTTCTCGTTTCTGCACTGTAAGGTTTCCGGGTCGGGTGCCTTGTTTTTGGGTCGGGCGTGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAACCGTGAACTGACTGTATTTTATGGGCAATACAGATGCTCTGGACCGGGCGCGGATTATGGCGGTCGAGTTCCATGGTCCAGAGAACTCACCGAGTCAGAAGCCAACCCGTTCCTCTCTCTTGATTTCATTAATGCAAATCAATGGCTTCCAAAGCACACTCATCTCACCATTAAACCACATTAA

Protein sequence

MTMSTLVKLLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKTKNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWLPKHTHLTIKPH
Homology
BLAST of Sed0017967 vs. NCBI nr
Match: XP_004146488.1 (probable pectinesterase 53 [Cucumis sativus] >KGN53279.1 hypothetical protein Csa_015141 [Cucumis sativus])

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 326/371 (87.87%), Postives = 349/371 (94.07%), Query Frame = 0

Query: 11  LLVAVMLSCAAASSGRNWTVGGSGRGMRK-VEDDYLKWIRRMGSFKHSLFQKTKNKFKPC 70
           LL+A++L  AA    +NWT GG GRGMR  V+D+Y KWIR MGSFKHSLFQ TKNKFKPC
Sbjct: 12  LLIAIILPAAA----QNWTAGG-GRGMRSTVQDEYFKWIRHMGSFKHSLFQNTKNKFKPC 71

Query: 71  LTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGEG 130
           LTLKVSKN K GGFRS+QKAVNSLP++N CRV IHV AG+YREKVEIPATMSYIWVEGEG
Sbjct: 72  LTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG 131

Query: 131 AEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQA 190
           AEKTIIEWGDTADH+G+NG PMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQA
Sbjct: 132 AEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQA 191

Query: 191 VAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA 250
           VAFRI+GDAAAFISC+F+GAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA
Sbjct: 192 VAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA 251

Query: 251 ITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIITP 310
           IT++YGALTAQKR+S+LEETGFSFLHCKVSGSGAL+LGRAWGSFSRVVFAYTFMDKIITP
Sbjct: 252 ITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITP 311

Query: 311 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWLP 370
           TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELT+SEANPFLSLDFINANQWLP
Sbjct: 312 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWLP 371

Query: 371 K-HTHLTIKPH 380
             HTHL++KPH
Sbjct: 372 NYHTHLSLKPH 377

BLAST of Sed0017967 vs. NCBI nr
Match: XP_008465244.1 (PREDICTED: probable pectinesterase 53 [Cucumis melo])

HSP 1 Score: 681.8 bits (1758), Expect = 3.4e-192
Identity = 326/372 (87.63%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 9   LLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKTKNKFKP 68
           L LL+A++L  AA    +NWT GG GRGMR V+D+YLKWIR MGSFKHSLFQ TKNKFKP
Sbjct: 9   LFLLIAIILPAAA----QNWTAGG-GRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKP 68

Query: 69  CLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGE 128
           CLTLKVSKN K GGFRS+QKAVNSLP++N CRV IHV AG+YREKVEIPATMSYIWVEGE
Sbjct: 69  CLTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGE 128

Query: 129 GAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQ 188
           GAEKTIIEWGDTADH+G+NG PMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQ
Sbjct: 129 GAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQ 188

Query: 189 AVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248
           AVAFRI+GDAAAFISC+F+GAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH
Sbjct: 189 AVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248

Query: 249 AITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIIT 308
           AIT++YGALTAQKR+S+LEETGFSFLHCKVSGSGAL+LGRAWGSFSRVVFAYTFMDKIIT
Sbjct: 249 AITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIIT 308

Query: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWL 368
           PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+SEANPFLSLDFINA QWL
Sbjct: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAKQWL 368

Query: 369 PK-HTHLTIKPH 380
           P  HTHL+ KPH
Sbjct: 369 PNYHTHLSHKPH 375

BLAST of Sed0017967 vs. NCBI nr
Match: KAG6600598.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 678.3 bits (1749), Expect = 3.8e-191
Identity = 327/379 (86.28%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 3   MSTLVKLLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKT 62
           M  L  L+L+V ++LS AA S G+NWTVGG GR MRKVED+YLKWIR MGSFKHSLFQ T
Sbjct: 38  MRMLNFLVLVVTMLLSGAAVSHGQNWTVGG-GR-MRKVEDEYLKWIRHMGSFKHSLFQNT 97

Query: 63  KNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSY 122
           KNKFKPCLTL+VSK PK GGFRSVQKA+NSLP++N CRVVIHV AG+YREKVEIPA MSY
Sbjct: 98  KNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNRCRVVIHVAAGIYREKVEIPAMMSY 157

Query: 123 IWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPS 182
           IWVEGEGAEKTIIEW DTADH+GDNG P+GTF SATFAVNSPFFIARNITFKNKAR PPS
Sbjct: 158 IWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPS 217

Query: 183 GALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 242
           GALGKQAVAFRITGDAAAFISC+F+GAQDTLYDH+GRHYFKDCYIEGSVDFVFGDGLSIY
Sbjct: 218 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 277

Query: 243 DSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTF 302
           + CHLHAITD+YGALTAQKR+SLLEETGFSFL+CKVSGSGAL+LGRAWGSFSRVVFAYTF
Sbjct: 278 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 337

Query: 303 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFI 362
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELTESEANPF+SLDFI
Sbjct: 338 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFI 397

Query: 363 NANQWLPKHTHLTIK--PH 380
            A +WLPK THL IK  PH
Sbjct: 398 GAKEWLPKRTHLAIKSPPH 414

BLAST of Sed0017967 vs. NCBI nr
Match: KAG7031236.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 326/379 (86.02%), Postives = 349/379 (92.08%), Query Frame = 0

Query: 3   MSTLVKLLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKT 62
           M  L   +L+V ++LS AA S G+NWTVGG GR MRKVED+YLKWIR MGSFKHSLFQ T
Sbjct: 1   MRMLNFFVLVVTMLLSGAAVSHGQNWTVGG-GR-MRKVEDEYLKWIRHMGSFKHSLFQNT 60

Query: 63  KNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSY 122
           KNKFKPCLTL+VSK PK GGFRSVQKA+NSLP++N CRVVIHV AG+YREKVEIPA MSY
Sbjct: 61  KNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNRCRVVIHVAAGIYREKVEIPAMMSY 120

Query: 123 IWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPS 182
           IWVEGEGAEKTIIEW DTADH+GDNG P+GTF SATFAVNSPFFIARNITFKNKAR PPS
Sbjct: 121 IWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPS 180

Query: 183 GALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 242
           GALGKQAVAFRITGDAAAFISC+F+GAQDTLYDH+GRHYFKDCYIEGSVDFVFGDGLSIY
Sbjct: 181 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 240

Query: 243 DSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTF 302
           + CHLHAITD+YGALTAQKR+SLLEETGFSFL+CKVSGSGAL+LGRAWGSFSRVVFAYTF
Sbjct: 241 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 300

Query: 303 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFI 362
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELTESEANPF+SLDFI
Sbjct: 301 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFI 360

Query: 363 NANQWLPKHTHLTIK--PH 380
            A +WLPK THL IK  PH
Sbjct: 361 GAKEWLPKRTHLAIKSPPH 377

BLAST of Sed0017967 vs. NCBI nr
Match: XP_023527790.1 (probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.0 bits (1743), Expect = 1.9e-190
Identity = 325/379 (85.75%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 3   MSTLVKLLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKT 62
           M  L   +L+V ++LS AA S G+NWTVGG GR MRKVED+YLKWIR MGSFKHSLFQ T
Sbjct: 1   MRVLNFFVLVVMMLLSGAAVSHGQNWTVGG-GR-MRKVEDEYLKWIRHMGSFKHSLFQNT 60

Query: 63  KNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSY 122
           KNKFKPCLTL+VSK PK GGFRSVQKA+NSLP++N CRVVIHV AG+YREKVEIPA MSY
Sbjct: 61  KNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSY 120

Query: 123 IWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPS 182
           IWVEGEGAEKTIIEW DTADH+GDNG P+GTF SATFAVNSPFF+ARNITFKNKAR PPS
Sbjct: 121 IWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFVARNITFKNKARAPPS 180

Query: 183 GALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 242
           GALGKQAVAFRITGDAAAFISC+F+GAQDTLYDH+GRHYFKDCYIEGSVDFVFGDGLSIY
Sbjct: 181 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 240

Query: 243 DSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTF 302
           + CHLHAITD+YGALTAQKR+SLLEETGFSFL CKVSGSGAL+LGRAWGSFSRVVFAYTF
Sbjct: 241 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKVSGSGALYLGRAWGSFSRVVFAYTF 300

Query: 303 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFI 362
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELTESEANPF+SLDFI
Sbjct: 301 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFI 360

Query: 363 NANQWLPKHTHLTIK--PH 380
            A +WLPK THL IK  PH
Sbjct: 361 GAKEWLPKRTHLAIKSPPH 377

BLAST of Sed0017967 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 6.6e-130
Identity = 207/328 (63.11%), Postives = 262/328 (79.88%), Query Frame = 0

Query: 41  EDDYLKWIRRMGSFKHSLFQKTKNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCR 100
           ED+++KW+R +GS KHS+F+  KNK  P  TL V K    G F  +Q A++SLP++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 101 VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFA 160
           VVI V AGVY+EKV IP   ++I +EGEGAEKT +EWGDTA      G+PMGT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 161 VNSPFFIARNITFKNKARLPPSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRH 220
           VNSPFF+A+NITF+N   +P  GA+GKQAVA R++ D AAF  C+ +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 221 YFKDCYIEGSVDFVFGDGLSIYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSG 280
           Y+KDCYIEGSVDF+FG+ LS+Y+ CH+HAI D  GA+TAQ R S+LE+TGFSF+ CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 281 SGALFLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 340
           +G L+LGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 341 RVPWSRELTESEANPFLSLDFINANQWL 369
           RV W+RELT+ EA PFLSL FI+ ++W+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Sed0017967 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.6e-78
Identity = 138/287 (48.08%), Postives = 187/287 (65.16%), Query Frame = 0

Query: 83  FRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 142
           FRSVQ AV+S+P  N+  + I +  G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 143 HVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRITGDAAAFI 202
            +G NG  + T+ +A+  V + +F ARNI+F N A  P  G  G QAVAFRI+GD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 203 SCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITDTYGALTAQKR 262
            C F GAQDTL D  GRHYFK+CYIEGS+DF+FG+G S+Y  C LH+I   +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 263 DSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 322
               E+TGF+F+ C+V+G+G L++GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 323 LTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWL 369
            T F+G Y C GPGA     V W+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Sed0017967 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.7e-77
Identity = 144/319 (45.14%), Postives = 211/319 (66.14%), Query Frame = 0

Query: 61  KTKNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATM 120
           + ++++K  L L V  +   G F +VQ A++ +P L+S + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVDLH-GCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 121 SYIWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLP 180
           + + ++G G + T IEW DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 181 PSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLS 240
             G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK+C+I+GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 241 IYDSCHLHAI-----TDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSR 300
           +Y  C +++I     +   G++TAQ R S  E++GFSF++CK+ GSG + LGRAWG+++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 301 VVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANP 360
           VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT+SEA+ 
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 361 FLSLDFINANQWLPKHTHL 375
           F+ + FI+ ++WL +HT++
Sbjct: 380 FIDVSFIDGDEWL-RHTNI 390

BLAST of Sed0017967 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.3e-74
Identity = 160/372 (43.01%), Postives = 214/372 (57.53%), Query Frame = 0

Query: 9   LLLLVAVML----SCAAASSGRNWTVGG-SGRGMRKVEDDYLKWIRRMGSFKHSLFQKTK 68
           LLL   VML    SCA      N      S   +R VED     I R  S K +    T 
Sbjct: 11  LLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGR---IERSFSIKENSNWVTT 70

Query: 69  NKFKPCLTLKVSK---NPKPGGFRS--VQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPA 128
           N         V +     K GG  S  VQ AV+ +P  NS RV I +  G+YREKV +P 
Sbjct: 71  NANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPK 130

Query: 129 TMSYIWVEGEG--AEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNK 188
           +  YI   G    A  T+I W D A  +G +G  +GT+ +A+ ++ S FF A  ITF+N 
Sbjct: 131 SKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENT 190

Query: 189 ARLPPSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFG 248
             +  +G  G+QAVA RI GD A F   + +G+QDTL+D  G HYF  CYI+G+VDF+FG
Sbjct: 191 V-VAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFG 250

Query: 249 DGLSIYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRV 308
           +  S+Y  C +H+    YGA+ A  RDS  E+TGFSF++C +SG+G ++LGRAWG++SR 
Sbjct: 251 NAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRT 310

Query: 309 VFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPF 368
           V++  F+  IITP GW +W    R+  V +G+Y C G GA+ GGRVPWS+ LT  E  PF
Sbjct: 311 VYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPF 370

BLAST of Sed0017967 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.2e-72
Identity = 138/316 (43.67%), Postives = 188/316 (59.49%), Query Frame = 0

Query: 66  FKPCLTLKVSKNPKPGGFRSVQKAVNSLP--VLNSCRVVIHVGAGVYREKVEIPATMSYI 125
           F   + ++V ++ K G F  +Q+A+ S+P  + NS    I V  G+YREKV IPA   YI
Sbjct: 45  FSTAILIRVDQSGK-GDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 104

Query: 126 WVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 185
            + G  A  T + W D  D             S T  + +  F+ R +T +NK      G
Sbjct: 105 TLSGTQASNTFLIWSDGED----------ILESPTLTIFASDFVCRFLTIQNK-----FG 164

Query: 186 ALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 245
             G+ AVA R+  D AAF  C     QDTL D  G HYFK+CYIEG+ DF+ G   S+Y+
Sbjct: 165 TAGR-AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYE 224

Query: 246 SCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFM 305
            CHLH+++   G++TAQ R S  E++GF+FL CK++GSG+ FLGR WG++SRVVFAY+F 
Sbjct: 225 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 284

Query: 306 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFIN 365
             ++ P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ EA  FLS DFI 
Sbjct: 285 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 343

Query: 366 ANQWL-PKHTHLTIKP 379
              WL P  +H    P
Sbjct: 345 GKDWLRPAPSHFKNAP 343

BLAST of Sed0017967 vs. ExPASy TrEMBL
Match: A0A0A0KWM1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 7.4e-193
Identity = 326/371 (87.87%), Postives = 349/371 (94.07%), Query Frame = 0

Query: 11  LLVAVMLSCAAASSGRNWTVGGSGRGMRK-VEDDYLKWIRRMGSFKHSLFQKTKNKFKPC 70
           LL+A++L  AA    +NWT GG GRGMR  V+D+Y KWIR MGSFKHSLFQ TKNKFKPC
Sbjct: 12  LLIAIILPAAA----QNWTAGG-GRGMRSTVQDEYFKWIRHMGSFKHSLFQNTKNKFKPC 71

Query: 71  LTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGEG 130
           LTLKVSKN K GGFRS+QKAVNSLP++N CRV IHV AG+YREKVEIPATMSYIWVEGEG
Sbjct: 72  LTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG 131

Query: 131 AEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQA 190
           AEKTIIEWGDTADH+G+NG PMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQA
Sbjct: 132 AEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQA 191

Query: 191 VAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA 250
           VAFRI+GDAAAFISC+F+GAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA
Sbjct: 192 VAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHA 251

Query: 251 ITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIITP 310
           IT++YGALTAQKR+S+LEETGFSFLHCKVSGSGAL+LGRAWGSFSRVVFAYTFMDKIITP
Sbjct: 252 ITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITP 311

Query: 311 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWLP 370
           TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELT+SEANPFLSLDFINANQWLP
Sbjct: 312 TGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWLP 371

Query: 371 K-HTHLTIKPH 380
             HTHL++KPH
Sbjct: 372 NYHTHLSLKPH 377

BLAST of Sed0017967 vs. ExPASy TrEMBL
Match: A0A1S3CNF2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 326/372 (87.63%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 9   LLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKTKNKFKP 68
           L LL+A++L  AA    +NWT GG GRGMR V+D+YLKWIR MGSFKHSLFQ TKNKFKP
Sbjct: 9   LFLLIAIILPAAA----QNWTAGG-GRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKP 68

Query: 69  CLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGE 128
           CLTLKVSKN K GGFRS+QKAVNSLP++N CRV IHV AG+YREKVEIPATMSYIWVEGE
Sbjct: 69  CLTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGE 128

Query: 129 GAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQ 188
           GAEKTIIEWGDTADH+G+NG PMGTFASATFAVNSPFFIA NITFKNKARLPPSGALGKQ
Sbjct: 129 GAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQ 188

Query: 189 AVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248
           AVAFRI+GDAAAFISC+F+GAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH
Sbjct: 189 AVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248

Query: 249 AITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIIT 308
           AIT++YGALTAQKR+S+LEETGFSFLHCKVSGSGAL+LGRAWGSFSRVVFAYTFMDKIIT
Sbjct: 249 AITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIIT 308

Query: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWL 368
           PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+SEANPFLSLDFINA QWL
Sbjct: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAKQWL 368

Query: 369 PK-HTHLTIKPH 380
           P  HTHL+ KPH
Sbjct: 369 PNYHTHLSHKPH 375

BLAST of Sed0017967 vs. ExPASy TrEMBL
Match: A0A6J1J245 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 9.0e-191
Identity = 319/373 (85.52%), Postives = 346/373 (92.76%), Query Frame = 0

Query: 9   LLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKTKNKFKP 68
           +L+++ ++L  AA S G+NWTVGG   GMR+VED+YLKWIR MGSFKHSLFQ TKNKFKP
Sbjct: 8   VLVVMIMLLPGAAVSYGQNWTVGGG--GMRRVEDEYLKWIRHMGSFKHSLFQNTKNKFKP 67

Query: 69  CLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGE 128
           CLTL+VSK PKPGGFRSVQKA+NSLP++N CRVVIHV AG+YREKVEIPA MSYIWVEGE
Sbjct: 68  CLTLRVSKKPKPGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSYIWVEGE 127

Query: 129 GAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQ 188
           GAEKT+IEW DTADH+GDNG P+GTF SATFAVNSPFFIARNITFKNKAR PPSGALGKQ
Sbjct: 128 GAEKTVIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPSGALGKQ 187

Query: 189 AVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248
           AVAFRITGDAAAFISC+F+GAQDTLYDH+GRHYFKDCYIEGSVDFVFGDGLSIY+ CHLH
Sbjct: 188 AVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYEGCHLH 247

Query: 249 AITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIIT 308
           AITD+YGALTAQKR+SLLEETGFSFL+CKVSGSGAL+LGRAWGSFSRVVFAYTFMDKIIT
Sbjct: 248 AITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIIT 307

Query: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWL 368
           PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELTE EANPF+SLDFI A +WL
Sbjct: 308 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTEPEANPFVSLDFIGAKEWL 367

Query: 369 PKHTHLTIK--PH 380
           PK THL IK  PH
Sbjct: 368 PKRTHLAIKSPPH 378

BLAST of Sed0017967 vs. ExPASy TrEMBL
Match: A0A6J1FW15 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.4e-190
Identity = 325/379 (85.75%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 3   MSTLVKLLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKT 62
           M  L   +L+V ++LS AA S G+NWTVGG GR MRKVED+YLKWIR MGSFKHSLFQ T
Sbjct: 1   MRMLNFFVLVVMMLLSGAAVSHGQNWTVGG-GR-MRKVEDEYLKWIRHMGSFKHSLFQNT 60

Query: 63  KNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSY 122
           KNKFKPCLTL+VSK PK GGFRSVQKA+NSLP++N CRVVIHV AG+YREKVEIPA MSY
Sbjct: 61  KNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSY 120

Query: 123 IWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPS 182
           IWVEGEGAEKTIIEW DTADH+GDNG P+GTF SATFAVNSPFFIARNITFKNKAR PPS
Sbjct: 121 IWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPS 180

Query: 183 GALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 242
           GALGKQAVAFRITGDAAAFISC+F+GAQDTLYDH+GRHYFKDCYIEGSVDFVFGDGLSIY
Sbjct: 181 GALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIY 240

Query: 243 DSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTF 302
           + CHLHAITD+YGALTAQKR+SLLEETGFSFL+CKVSGSGAL+LGRAWGSFSRVVFAYTF
Sbjct: 241 EGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 300

Query: 303 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFI 362
           MDKIITPTGWYNWGDKN ELTVFYGQYRCSGPGADYGGRV WSRELTESEANPF+SLDFI
Sbjct: 301 MDKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFVSLDFI 360

Query: 363 NANQWLPKHTHLTIK--PH 380
            A +WLPK THL IK  PH
Sbjct: 361 GAKEWLPKRTHLAIKSPPH 377

BLAST of Sed0017967 vs. ExPASy TrEMBL
Match: A0A5A7TMQ1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.8e-184
Identity = 317/380 (83.42%), Postives = 341/380 (89.74%), Query Frame = 0

Query: 9   LLLLVAVMLSCAAASSGRNWTVGGSGRGMRKVEDDYLKWIRRMGSFKHSLFQKTKNKFKP 68
           L LL+A++L  AA    +NWT GG GRGMR V+D+YLKWIR MGSFKHSLFQ TKNKFKP
Sbjct: 9   LFLLIAIILPAAA----QNWTAGG-GRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKP 68

Query: 69  CLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGE 128
           CLTLKVSKN K GGFRS+QKAVNSLP++N CRV IHV AG+YREKVEIPATMSYIWVEGE
Sbjct: 69  CLTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGE 128

Query: 129 GAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFK--------NKARLP 188
           GAEKTIIEWGDTADH+G+NG PMGTFASATFAVNSPFFIA NITFK          + LP
Sbjct: 129 GAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKVLTFSSFFLSSLLP 188

Query: 189 PSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLS 248
              +LGKQAVAFRI+GDAA FISC+F+GAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLS
Sbjct: 189 LKFSLGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLS 248

Query: 249 IYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAY 308
           IYDSCHLHAIT++YGALTAQKR+S+LEETGFSFLHCKVSGSGAL+LGRAWGSFSRVVFAY
Sbjct: 249 IYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAY 308

Query: 309 TFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLD 368
           TFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+SEANPFLSLD
Sbjct: 309 TFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLD 368

Query: 369 FINANQWLPK-HTHLTIKPH 380
           FINA QWLP  HTHL+ KPH
Sbjct: 369 FINAKQWLPNYHTHLSHKPH 383

BLAST of Sed0017967 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.7e-131
Identity = 207/328 (63.11%), Postives = 262/328 (79.88%), Query Frame = 0

Query: 41  EDDYLKWIRRMGSFKHSLFQKTKNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCR 100
           ED+++KW+R +GS KHS+F+  KNK  P  TL V K    G F  +Q A++SLP++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 101 VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFA 160
           VVI V AGVY+EKV IP   ++I +EGEGAEKT +EWGDTA      G+PMGT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 161 VNSPFFIARNITFKNKARLPPSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRH 220
           VNSPFF+A+NITF+N   +P  GA+GKQAVA R++ D AAF  C+ +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 221 YFKDCYIEGSVDFVFGDGLSIYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSG 280
           Y+KDCYIEGSVDF+FG+ LS+Y+ CH+HAI D  GA+TAQ R S+LE+TGFSF+ CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 281 SGALFLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 340
           +G L+LGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 341 RVPWSRELTESEANPFLSLDFINANQWL 369
           RV W+RELT+ EA PFLSL FI+ ++W+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of Sed0017967 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 1.9e-79
Identity = 138/287 (48.08%), Postives = 187/287 (65.16%), Query Frame = 0

Query: 83  FRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 142
           FRSVQ AV+S+P  N+  + I +  G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 143 HVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSGALGKQAVAFRITGDAAAFI 202
            +G NG  + T+ +A+  V + +F ARNI+F N A  P  G  G QAVAFRI+GD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 203 SCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITDTYGALTAQKR 262
            C F GAQDTL D  GRHYFK+CYIEGS+DF+FG+G S+Y  C LH+I   +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 263 DSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 322
               E+TGF+F+ C+V+G+G L++GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 323 LTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFINANQWL 369
            T F+G Y C GPGA     V W+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Sed0017967 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-78
Identity = 144/319 (45.14%), Postives = 211/319 (66.14%), Query Frame = 0

Query: 61  KTKNKFKPCLTLKVSKNPKPGGFRSVQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPATM 120
           + ++++K  L L V  +   G F +VQ A++ +P L+S + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVDLH-GCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 121 SYIWVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLP 180
           + + ++G G + T IEW DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 181 PSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLS 240
             G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK+C+I+GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 241 IYDSCHLHAI-----TDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSR 300
           +Y  C +++I     +   G++TAQ R S  E++GFSF++CK+ GSG + LGRAWG+++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 301 VVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANP 360
           VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT+SEA+ 
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 361 FLSLDFINANQWLPKHTHL 375
           F+ + FI+ ++WL +HT++
Sbjct: 380 FIDVSFIDGDEWL-RHTNI 390

BLAST of Sed0017967 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.6e-75
Identity = 160/372 (43.01%), Postives = 214/372 (57.53%), Query Frame = 0

Query: 9   LLLLVAVML----SCAAASSGRNWTVGG-SGRGMRKVEDDYLKWIRRMGSFKHSLFQKTK 68
           LLL   VML    SCA      N      S   +R VED     I R  S K +    T 
Sbjct: 11  LLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGR---IERSFSIKENSNWVTT 70

Query: 69  NKFKPCLTLKVSK---NPKPGGFRS--VQKAVNSLPVLNSCRVVIHVGAGVYREKVEIPA 128
           N         V +     K GG  S  VQ AV+ +P  NS RV I +  G+YREKV +P 
Sbjct: 71  NANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPK 130

Query: 129 TMSYIWVEGEG--AEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNK 188
           +  YI   G    A  T+I W D A  +G +G  +GT+ +A+ ++ S FF A  ITF+N 
Sbjct: 131 SKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENT 190

Query: 189 ARLPPSGALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFG 248
             +  +G  G+QAVA RI GD A F   + +G+QDTL+D  G HYF  CYI+G+VDF+FG
Sbjct: 191 V-VAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFG 250

Query: 249 DGLSIYDSCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRV 308
           +  S+Y  C +H+    YGA+ A  RDS  E+TGFSF++C +SG+G ++LGRAWG++SR 
Sbjct: 251 NAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRT 310

Query: 309 VFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPF 368
           V++  F+  IITP GW +W    R+  V +G+Y C G GA+ GGRVPWS+ LT  E  PF
Sbjct: 311 VYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPF 370

BLAST of Sed0017967 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 272.7 bits (696), Expect = 4.4e-73
Identity = 138/316 (43.67%), Postives = 188/316 (59.49%), Query Frame = 0

Query: 66  FKPCLTLKVSKNPKPGGFRSVQKAVNSLP--VLNSCRVVIHVGAGVYREKVEIPATMSYI 125
           F   + ++V ++ K G F  +Q+A+ S+P  + NS    I V  G+YREKV IPA   YI
Sbjct: 45  FSTAILIRVDQSGK-GDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 104

Query: 126 WVEGEGAEKTIIEWGDTADHVGDNGHPMGTFASATFAVNSPFFIARNITFKNKARLPPSG 185
            + G  A  T + W D  D             S T  + +  F+ R +T +NK      G
Sbjct: 105 TLSGTQASNTFLIWSDGED----------ILESPTLTIFASDFVCRFLTIQNK-----FG 164

Query: 186 ALGKQAVAFRITGDAAAFISCKFMGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 245
             G+ AVA R+  D AAF  C     QDTL D  G HYFK+CYIEG+ DF+ G   S+Y+
Sbjct: 165 TAGR-AVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYE 224

Query: 246 SCHLHAITDTYGALTAQKRDSLLEETGFSFLHCKVSGSGALFLGRAWGSFSRVVFAYTFM 305
            CHLH+++   G++TAQ R S  E++GF+FL CK++GSG+ FLGR WG++SRVVFAY+F 
Sbjct: 225 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 284

Query: 306 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTESEANPFLSLDFIN 365
             ++ P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ EA  FLS DFI 
Sbjct: 285 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 343

Query: 366 ANQWL-PKHTHLTIKP 379
              WL P  +H    P
Sbjct: 345 GKDWLRPAPSHFKNAP 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146488.11.5e-19287.87probable pectinesterase 53 [Cucumis sativus] >KGN53279.1 hypothetical protein Cs... [more]
XP_008465244.13.4e-19287.63PREDICTED: probable pectinesterase 53 [Cucumis melo][more]
KAG6600598.13.8e-19186.28putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7031236.11.1e-19086.02putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023527790.11.9e-19085.75probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8VYZ36.6e-13063.11Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF32.6e-7848.08Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9ZQA31.7e-7745.14Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9FM792.3e-7443.01Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q9SIJ96.2e-7243.67Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWM17.4e-19387.87Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1[more]
A0A1S3CNF21.6e-19287.63Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1[more]
A0A6J1J2459.0e-19185.52Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1[more]
A0A6J1FW153.4e-19085.75Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1[more]
A0A5A7TMQ14.8e-18483.42Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 ... [more]
Match NameE-valueIdentityDescription
AT5G19730.14.7e-13163.11Pectin lyase-like superfamily protein [more]
AT5G47500.11.9e-7948.08Pectin lyase-like superfamily protein [more]
AT2G36710.11.2e-7845.14Pectin lyase-like superfamily protein [more]
AT5G55590.11.6e-7543.01Pectin lyase-like superfamily protein [more]
AT2G21610.14.4e-7343.67pectinesterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 80..364
e-value: 7.9E-71
score: 238.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 70..376
e-value: 3.2E-98
score: 331.0
NoneNo IPR availablePANTHERPTHR31321:SF103PECTINESTERASEcoord: 34..368
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 34..368
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 227..236
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 70..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0017967.1Sed0017967.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity