Sed0017952 (gene) Chayote v1

Overview
NameSed0017952
Typegene
OrganismSechium edule (Chayote v1)
Descriptionmethylsterol monooxygenase 1-2
LocationLG03: 8181253 .. 8186180 (-)
RNA-Seq ExpressionSed0017952
SyntenySed0017952
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAGTTTTGGAAGGGAGTGATAAATGGTGGATGCCATTCCCAATTATATATATATATAAAAACACAAACTCTCAAATAAACAAAACATTCAATTTCCAACCCATAGATTTTCACGCTCTACCTCTTTTTTCTTTTTCAAAATTCCCGCATTATTAATTTCATCCATTTCCGCCGCTTCTTAATCCCGATCCCAATCCCCATTCGCCGCCGGCGGATTCCGCCACCGTCATGTTGCCGTTCCACTCCCTCTCCGCCGCGCAGGCCGCCGTCGGCCGGAATCTCACCGTCCTCGAAACTCTCTGGTTCGATTACTCACACGACAAGTCCGATTACTTCCTCTATTGCCACAACATTCTGTTCCTCTTCTTGATCTTCTCTTTCATTCCCATTCCCCTCGTTTTCCTCGAGCTTTTTCACGTCTCTGGGATTTGTAGCTACAAGATTCAGCCCAAAGTCCGGTTGTCCTTCTCCGAGATCTTCCGCTGCTACAAGGATGTGATGCGGATGTTCTTCCTTGTCGTCGGCCCTCTTCAGCTCGTTTCATTCCCTTCCGTTCAGGTGAATCTTCCATTTCCATTTTCTAATTTCTGGTTGATGAATCTGGATTGTGATTGTTATTAGATAGTGCGTACATACATCTCAAAATGAATCGGTGAATGAGAGGAGTAGTCGCCTTCACCAATCTTGAATCTGAATCGGATCTCAATTTTATTTTAATGGACCGGATACCGCTGGTTTGGGCTTTAAATGGATGTACTGCCATATTAGATAGTGTGGGTATCCGTTCAAAATGAATCGGTGATGAGATAAGTAGTCCATATCACCAATCTTGAATCTGAATCGGGTCCTAATTTCATTTTATTGGACCGAATACTGTTGGCTCAGGTTTTAAATGGGTGTCGATACCATATTAGATAGTGTGAGCATCCATTCAAAATGAATTGACGACGAGAGGAGTAGTCACCTTCTTATAAATTGTTATGATGTGTTCACTTATTCTAAATTTCTAACCATTGTGATTGTGATTGATTGGTATTTTTTTTTTGGTTTTAAGATGCAATTGTGATTGATTGGTTTTTTTTTGGTTTATAGATGATTGGGATCAGGACAGGTTTGCCATTGCCTTCTGGGTGGGAAATTGTTTCCCAATTGGTGGTTTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTTCTCCATTGTAAATGGGGGTATGAGAAAATTCATCGGGTTCACCATGAATATACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGGCCCTTGGAATTCCCTCTTTTCTTGGTCCTGCTATTGTTCCTGGTCATATGATTACTCTCTGGTCGTGGATTGCTTTAAGACAGATTGAAGCCATTGAAACTCATAGTGGGTATGTATTGCTAAACCTATTATTTCTCTCTGTTACTTTTTCTTAATCTTGTGTTTTTATTCGACTGCGACAAAATGATGCCAACTTTGAATTCCTACATTCTTTTATTTATCCGCCAATGCCTTGTGTTAAAGTTACTTTTAATCTCAATGATCTTGAATGGTGGGTTACCTTTTGTTGAAGAGCACTGGGATCTGCTCTATTGCTTTCTTCTTTTTTTAATATAGGTGCATGTTTAGACCCAATATAGAGTTTCTATCATTACTGCATGAACTTATTATTGCATACTGAATCATTGACCCTACTAACTTGATATGATGAAAAATTGAAGTTTTTGTTGAGACATGATAAAAATTTGCCCTTCTTTAAGTTTCATAGAATCAACTTTCTTAGCGTTCATATTCTTCATAAAGTTATGGGAAAGGTCGTGATTCCTTTAACTTAGCTTAAGAGGACCCTATAAATCTTCTATGAGAAGCCCAGCCTAAGAAGCTTGATTCTTGTATACTAAACTTTTTAGGGCCCACTACTCCTTTAAGCTGTAAGATTTTGGACATCATTTCAAGAACTATGATTTAACATGCTATGTAGGTAGCAAATCTTGTGGGACCATAGACATTAGACTGTAGGCTTTAGAGAAACAACAGAGTGCCTATGTCAATGTATTTGCATCTATGTTAGGTAAGCACAATGACATTGAGGGATGTTGTTATGAAGTGATGCAGCACGATATCGATGGGCGATGATCATGAACAAGACCATAACTAAACCTTATTGGTTCAAACATTCTTTGCTTCAAGGGACCTTTAGCTTACAACCATTCCCAATCTTCCTCATCTTGCTCCATTGTGGTTGTTTATGAGGCTTCAGGTTAACTCCCTTGTGAAAGTATGATTTTTCGAAGTCAATGTTAAAATTTTGTGGCCTTGGGACCCTTGGGAGAAGGGAGAAAAAGGGAACAATATCTTCTGGCATGTGGTTTGCTTATCTTGAGCAATTAGTATGTTAATTTCATGGGCTACATAATCACACTAGTTATACAAACCCAAGTTTCGATGACCCATTGCTTGATACTCATACAATCTAAACACAAATCAATTGCTGAAGCTTTTGAACAAACCAGCCTTATTAAATTGTCACAGTTCCTCTTCCTGAGAAGAAGAATCAAAAGTAAGCTTTATTTTTCATGTGGACTGCACTTGTTTAAAACTGTTGAAAGAAAAGTAAATTAACATTTGAAAGAGGCTAGGTTACTCAAATAAGAATCCATTGATTTGACCTCCAAATTGTGTAGTTGGGCCAGAAATGAAAAATAATTGATGCCACGGCTTATGGCTTGGAATAAATGCGGTGTCAGGTAATCATTTTCTTTTGTCAAGTACGACATAGCTTCCCTCTTGGTGGGTTTTCTGTTGCCGAAAATCCCCTAAGCCTTTGATTATTACTTTTAAATTGCTGAAATTCGTTTGTTTTATCTCTTTCATCAAAGGCTGAGATTTGCATGAACATTGATGGCAAGTAGTTGTGTAACTTTTTTATGCTAACATAAATGTTGAGAAAAATATAAAGATGAAAGTTTTATATCAACAACATTTATCGGAAGTTGAAGGATATTCTTTTTATAGGTTGTGGGAGAATTGTTACAGTTAAATTGTCAATATTTTCACAAACATTAGTGTGAATGAACTGTCGACATTTTCATTCTTAATTAATGTTTTGCTTTTAAGTTAAATTGCCAGTTTAGTTCTTATATTCCAGCTGGGTTTCAACTTGTGGTCATTGTAGTTTTGAAAGTTCATTTATGTTAATAGTTTTCTTATGTGATAGAGAGGATTGATAAGCTGATTTGTAGTATAAAGCATACGTGATAGGTCAAATGGGTAAGTCCTACAGTATACTTCGAAATACGATTTCTCTCTCTACCATGTTCATGAAAACATATTGCAAGAGAATGTAGACTTCCAGTTACAACGCAGACGTTTTGGGAGATTTAACCTATCATAGACACTAAAATTTATAGAGTAATCTAGTGGTCAAGAGACGAGAAGGGTCTTGAGGAATTAAGAGGTGATGTGTTCAATCCATGGTAACCACCTACGTAGGAATTATTTTTCTACGAGTTTCTTTGACATCCAAATACTGTAGGGTCAAACGATATTTTCCATGACATTAGTTAAGGTGTGTGCTAACTGGTCCAAACACTTATGGATATCCAAAAAAAAAAAAAATTATAGATATCAGAGTTGAAATTTTAACCTTGTTTTTTTATACTAGTCAACTATTCATGACTCATGAGAATCCTATCCTTTCTCTTTACTTTTTAGCCTCTCTCTCCCTTTTTTTCCCCCAGATTGTGCTTTTATGCTTTTATGATGAGTGTTGAGATCATCCCTGATTTTATTTATTTAATATTATATTCAGGTATGATTTCCCCTGGAGTCTAACAAAATTTATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGTAATTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATGCTCTTGCTATAATTTTACTTTCCCACTTGTTCTTCCAGTAAAATCATGCTCCTAAATCTACTCATTTCCTTGCTTTCTCAGGGCTATCGCTATCAAAAGAATATCCTTAAGAAGGTAAGCATTTCGTGTTTGTCCCCACAAAAATTGACCGTATATGGTCACGACTTTGTGTCTTGTTTCAATTTTCTCAAAGTGGACATCAGATTTACCCGAATTCGAGCTTGGTTAGATGTCGTGTTCATTTGTTAAATTTGATTATGCTTAATTAAGGATGGTTAGCTTAAAAGCTCTTGCCATGGCCTTTTCCTGTTCTTTTTGGTTGGGTCCAATCTGTTCCATTTGTTCTTTATGTATAGAAGTTCTGAAACAGTCATAATTGGTTCTCAGCTCATGTTCCATTTGTCATTTGCATTGTTGTGTAGGAGGAAGTTGTTTGTGAATAAAAAGGCAAAAGAACGAACGATTTATGAATGCAACTGTTTCTCTTATGCTACCGGATCTATTCGAAACGCAGTATTATGCAGTCAGCAATATCGTGTGTTCTAACTGTTTGTTGTTGGTTTAATCAGTTGAAGGAAGAAGCAAAGAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAATTCGACTAGACAATGAAAAGAGATTCATGTTTGGAAGGACGGGATTTTTTTCGTGTCAGTCGCGACGTTACAGAATGTCTTATTTGAAGTTTACTTGATCGAGATCGACGAGATAGCTAGGGACTGGGATGAGATGGGATGGGATGGGAATTAACTTGGGGGTCTATAAAAAGTTTTTTTTTTTAAATTTTCTTTTCTTGTAAGCTTAATCTTCAGGCTGAGAATTCATATTCACCAGTTTCTTATGTGGTTTAACCTTATTGTGCAGCCAAAACTCTTAATATAAATATGTATTTTGCTTGT

mRNA sequence

GAGAAAGTTTTGGAAGGGAGTGATAAATGGTGGATGCCATTCCCAATTATATATATATATAAAAACACAAACTCTCAAATAAACAAAACATTCAATTTCCAACCCATAGATTTTCACGCTCTACCTCTTTTTTCTTTTTCAAAATTCCCGCATTATTAATTTCATCCATTTCCGCCGCTTCTTAATCCCGATCCCAATCCCCATTCGCCGCCGGCGGATTCCGCCACCGTCATGTTGCCGTTCCACTCCCTCTCCGCCGCGCAGGCCGCCGTCGGCCGGAATCTCACCGTCCTCGAAACTCTCTGGTTCGATTACTCACACGACAAGTCCGATTACTTCCTCTATTGCCACAACATTCTGTTCCTCTTCTTGATCTTCTCTTTCATTCCCATTCCCCTCGTTTTCCTCGAGCTTTTTCACGTCTCTGGGATTTGTAGCTACAAGATTCAGCCCAAAGTCCGGTTGTCCTTCTCCGAGATCTTCCGCTGCTACAAGGATGTGATGCGGATGTTCTTCCTTGTCGTCGGCCCTCTTCAGCTCGTTTCATTCCCTTCCGTTCAGATGATTGGGATCAGGACAGGTTTGCCATTGCCTTCTGGGTGGGAAATTGTTTCCCAATTGGTGGTTTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTTCTCCATTGTAAATGGGGGTATGAGAAAATTCATCGGGTTCACCATGAATATACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGGCCCTTGGAATTCCCTCTTTTCTTGGTCCTGCTATTGTTCCTGGTCATATGATTACTCTCTGGTCGTGGATTGCTTTAAGACAGATTGAAGCCATTGAAACTCATAGTGGGTATGATTTCCCCTGGAGTCTAACAAAATTTATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGTAATTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGCTATCGCTATCAAAAGAATATCCTTAAGAAGTTGAAGGAAGAAGCAAAGAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAATTCGACTAGACAATGAAAAGAGATTCATGTTTGGAAGGACGGGATTTTTTTCGTGTCAGTCGCGACGTTACAGAATGTCTTATTTGAAGTTTACTTGATCGAGATCGACGAGATAGCTAGGGACTGGGATGAGATGGGATGGGATGGGAATTAACTTGGGGGTCTATAAAAAGTTTTTTTTTTTAAATTTTCTTTTCTTGTAAGCTTAATCTTCAGGCTGAGAATTCATATTCACCAGTTTCTTATGTGGTTTAACCTTATTGTGCAGCCAAAACTCTTAATATAAATATGTATTTTGCTTGT

Coding sequence (CDS)

ATGTTGCCGTTCCACTCCCTCTCCGCCGCGCAGGCCGCCGTCGGCCGGAATCTCACCGTCCTCGAAACTCTCTGGTTCGATTACTCACACGACAAGTCCGATTACTTCCTCTATTGCCACAACATTCTGTTCCTCTTCTTGATCTTCTCTTTCATTCCCATTCCCCTCGTTTTCCTCGAGCTTTTTCACGTCTCTGGGATTTGTAGCTACAAGATTCAGCCCAAAGTCCGGTTGTCCTTCTCCGAGATCTTCCGCTGCTACAAGGATGTGATGCGGATGTTCTTCCTTGTCGTCGGCCCTCTTCAGCTCGTTTCATTCCCTTCCGTTCAGATGATTGGGATCAGGACAGGTTTGCCATTGCCTTCTGGGTGGGAAATTGTTTCCCAATTGGTGGTTTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTTCTCCATTGTAAATGGGGGTATGAGAAAATTCATCGGGTTCACCATGAATATACTGCTCCTATTGGATTTGCAGCTCCTTATGCTCATTGGGCTGAGGTTTTGGCCCTTGGAATTCCCTCTTTTCTTGGTCCTGCTATTGTTCCTGGTCATATGATTACTCTCTGGTCGTGGATTGCTTTAAGACAGATTGAAGCCATTGAAACTCATAGTGGGTATGATTTCCCCTGGAGTCTAACAAAATTTATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGTAATTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGCTATCGCTATCAAAAGAATATCCTTAAGAAGTTGAAGGAAGAAGCAAAGAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAATTCGACTAG

Protein sequence

MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLELFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD
Homology
BLAST of Sed0017952 vs. NCBI nr
Match: XP_023552163.1 (methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.8 bits (1501), Expect = 1.7e-162
Identity = 275/300 (91.67%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQ A+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           + H SGI  YKIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEE KSSEGSYYSTAEDVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of Sed0017952 vs. NCBI nr
Match: XP_022923513.1 (methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015639.1 Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581.6 bits (1498), Expect = 3.8e-162
Identity = 274/300 (91.33%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQ A+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           + H SGI  YKIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEE KSSEGSYYSTAEDVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of Sed0017952 vs. NCBI nr
Match: XP_022965134.1 (methylsterol monooxygenase 1-2 [Cucurbita maxima])

HSP 1 Score: 580.5 bits (1495), Expect = 8.4e-162
Identity = 273/300 (91.00%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHS+SAAQ A+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           + H SGI  +KIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEE KSSEGSYYSTAEDVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of Sed0017952 vs. NCBI nr
Match: XP_038876881.1 (methylsterol monooxygenase 1-2-like [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 3.5e-160
Identity = 271/300 (90.33%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQAA+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           L H SGI  YKIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQLVVYFMVEDYTNYWIHRFLHCKWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIISQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILK+LKEE K+ EG Y+STA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKQLKEEVKNIEGPYHSTAQDVKSD 300

BLAST of Sed0017952 vs. NCBI nr
Match: KAG6596833.1 (Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573.9 bits (1478), Expect = 7.9e-160
Identity = 271/300 (90.33%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQAA+GRNLT  ETLWF+YSHDKS+YFLYCHNILFLFLIFS IP+PL+FLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTYFETLWFNYSHDKSNYFLYCHNILFLFLIFSLIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           LFHVSGI  YKIQPKVRL  +EIFRCYKDVMRMFFLVVGPLQLVS+PS+ MIGIRTGLPL
Sbjct: 61  LFHVSGIHKYKIQPKVRLPSAEIFRCYKDVMRMFFLVVGPLQLVSYPSINMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYW+HRFLHC+WGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWVHRFLHCEWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAIETHSGYDFPWS+TK IPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSVTKLIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEEAK SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKISEASYYSTAQDVKSD 300

BLAST of Sed0017952 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 2.5e-132
Identity = 219/288 (76.04%), Postives = 252/288 (87.50%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+ RNLT LETLWFDYS  KSDY+LYCHNILFLFLIFS +P+PLVF+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LFHVSG--ICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGL 120
               +      YKIQPKV+ SFS +F+CYKDVM+MF LVVGPLQLVS+PS+QMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHH 240
           EVL LGIP+FLGPAI PGHMIT W WIALRQIEAIETHSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QK +L+++KE++K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

BLAST of Sed0017952 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.0e-130
Identity = 216/288 (75.00%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+GRNLT LETLWFDYS  KSDY+LYCHNILFLFL+FS +P+PLVF+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LFH-VSGICS-YKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGL 120
           L    SG+ + YKIQPKV  S S++F+CYKDVM MF LVVGPLQLVS+PS+QMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHH 240
           EVL LGIP+F+GPAI PGHMIT W WIALRQ+EAIETHSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QK +L+++KE +K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKS 288

BLAST of Sed0017952 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 7.0e-119
Identity = 195/285 (68.42%), Postives = 240/285 (84.21%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+GRNLT  ET+WFDYS  KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
                    +KIQ KV+ S S++F+CYK+VM++F LVVG LQ+VS+PS+QM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIP+FLGPAI PGH++T W WI+LRQ EAIETHSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNIL-KKLKEEAK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  K +L  ++KEEA+
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of Sed0017952 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 8.6e-117
Identity = 199/297 (67.00%), Postives = 242/297 (81.48%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLP+ + + A+AA+GR +T  E+LWF YS    DY L+ HNILFLF++F+  P+P+  LE
Sbjct: 1   MLPYATAAEAEAALGRAMTAAESLWFRYSAGIPDYVLFWHNILFLFVVFTLAPLPVALLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           L   + +  +K+QPKVRLS  E FRCY+DVMR+FFLV+GPLQLVS+P+V+M+GI TGLPL
Sbjct: 61  LRAPAAVGPFKLQPKVRLSREEFFRCYRDVMRLFFLVIGPLQLVSYPTVKMVGIHTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  E+ +QL+VYF+VEDY NYWIHR LH +WGYEKIHRVHHE+TAPIGFAAPYAHWAEV
Sbjct: 121 PSLGEMAAQLLVYFLVEDYLNYWIHRLLHGEWGYEKIHRVHHEFTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSF+GPA+ PGHMIT W WI LRQ+EAIETHSG+DFP++LTK+IPFYGGA++HDY
Sbjct: 181 LILGIPSFVGPALAPGHMITFWLWIVLRQMEAIETHSGFDFPFNLTKYIPFYGGAEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLK-------EEAKSSEGSY 291
           HHYVG QS SNFASVFTYCDY+YGTDKGYRY K    K+K       E+A S+  SY
Sbjct: 241 HHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMKALGQTEGEKADSNGLSY 297

BLAST of Sed0017952 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.1e-54
Identity = 111/250 (44.40%), Postives = 151/250 (60.40%), Query Frame = 0

Query: 33  SDYFLYCHNILFLF-LIFSFIPIPLVFLELFHVSGICSYKIQPKVRLSFSEIFRCYKDVM 92
           SD+ L C     L   +F    +P +FLE      +  YKIQ K     ++  +C   ++
Sbjct: 17  SDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNNTPAAQ-GKCITRLL 76

Query: 93  RMFFLVVGPLQLVSFPSVQMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCK 152
              F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++ED+  YW HR LH K
Sbjct: 77  LYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSK 136

Query: 153 WGYEKIHRVHHEYTAPIGFAAPYAHWAEVLALGIPSFLGPAIVPGHMITLWSWIALRQIE 212
           W Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H+ITLW W+ LR +E
Sbjct: 137 WLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLE 196

Query: 213 AIETHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRY 272
            +E H GY FPWSL+ F+P YGGAD HDYHH +      N++S F Y D+I+GTDKGYR 
Sbjct: 197 TVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRR 256

Query: 273 QKNILKKLKE 282
               LK LKE
Sbjct: 257 ----LKTLKE 259

BLAST of Sed0017952 vs. ExPASy TrEMBL
Match: A0A6J1E9U9 (methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.8e-162
Identity = 274/300 (91.33%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQ A+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           + H SGI  YKIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGG+DHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEE KSSEGSYYSTAEDVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of Sed0017952 vs. ExPASy TrEMBL
Match: A0A6J1HQ57 (methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.1e-162
Identity = 273/300 (91.00%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHS+SAAQ A+GRNLT  ETLWF+YSHDKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           + H SGI  +KIQPKVRL FSEIFRCYKDVMRMFFLVVGPLQLVSFPS+QMIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEE KSSEGSYYSTAEDVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of Sed0017952 vs. ExPASy TrEMBL
Match: A0A6J1FUF2 (methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC111448229 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.5e-160
Identity = 271/300 (90.33%), Postives = 283/300 (94.33%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQAA+GRNLT  ETLWF+YSHDKS YFLYCHNILFLFLIFS IP+PL+FLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTSFETLWFNYSHDKSSYFLYCHNILFLFLIFSLIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           LFHVSGI  YKIQPKVRL  +EIFRCYKDVMRMFFLVVGPLQLVS+PS+ MIGIRTGLPL
Sbjct: 61  LFHVSGIHKYKIQPKVRLPSAEIFRCYKDVMRMFFLVVGPLQLVSYPSINMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEIVSQLVVYFMVEDYTNYW+HRFLHC+WGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWVHRFLHCEWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAIETHSGYDFPWS+TK IPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSVTKLIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK ILKKLKEEAK SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKISEASYYSTAQDVKSD 300

BLAST of Sed0017952 vs. ExPASy TrEMBL
Match: A0A6J1L2B1 (methylsterol monooxygenase 1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111498458 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.0e-157
Identity = 269/300 (89.67%), Postives = 282/300 (94.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQAA+GRNLT  ETLWF+YSHDKS+YFLYCHNILFLFLIFS IP+PL+FLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTYFETLWFNYSHDKSNYFLYCHNILFLFLIFSLIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           LFHVSGI  YKIQPKVRL  +EIFRCYKDVMRMFFLVVGPLQLVS+PS+ MIGIRTGLPL
Sbjct: 61  LFHVSGIHKYKIQPKVRLPSAEIFRCYKDVMRMFFLVVGPLQLVSYPSINMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG EIVSQLVVYFMVEDYTNYW+HRFLHC+WGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGGEIVSQLVVYFMVEDYTNYWVHRFLHCEWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAIETHSGYDFPWS+TK IPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSVTKLIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQK ILKKLKEEAK SE SYYSTA+DVK D
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEAKISEASYYSTAQDVKSD 300

BLAST of Sed0017952 vs. ExPASy TrEMBL
Match: A0A0A0L5P8 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.0e-157
Identity = 266/300 (88.67%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           MLPFHSLSAAQA++GRNLT  ETLWF+YS+DKSDYFL+CHNILFLFLIFSFIP+PL+FLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
           L H +GI  YKIQPKVRL F+EIFRCYKDVMRMFFLVVGPLQLVSFPS++MIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIPSFLGPA+VPGHMIT W WIALRQIEAI+THSGYDFPWSLTKFIPFYGGADHHDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSSEGSYYSTAEDVKFD 300
           HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQK IL+KLKEE K+SE SYY+TA++VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

BLAST of Sed0017952 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 473.0 bits (1216), Expect = 1.8e-133
Identity = 219/288 (76.04%), Postives = 252/288 (87.50%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+ RNLT LETLWFDYS  KSDY+LYCHNILFLFLIFS +P+PLVF+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  LFHVSG--ICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGL 120
               +      YKIQPKV+ SFS +F+CYKDVM+MF LVVGPLQLVS+PS+QMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHH 240
           EVL LGIP+FLGPAI PGHMIT W WIALRQIEAIETHSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QK +L+++KE++K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

BLAST of Sed0017952 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 466.1 bits (1198), Expect = 2.2e-131
Identity = 216/288 (75.00%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+GRNLT LETLWFDYS  KSDY+LYCHNILFLFL+FS +P+PLVF+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  LFH-VSGICS-YKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGL 120
           L    SG+ + YKIQPKV  S S++F+CYKDVM MF LVVGPLQLVS+PS+QMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHH 240
           EVL LGIP+F+GPAI PGHMIT W WIALRQ+EAIETHSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNILKKLKEEAKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QK +L+++KE +K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKS 288

BLAST of Sed0017952 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-120
Identity = 195/285 (68.42%), Postives = 240/285 (84.21%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+GRNLT  ET+WFDYS  KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
                    +KIQ KV+ S S++F+CYK+VM++F LVVG LQ+VS+PS+QM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIP+FLGPAI PGH++T W WI+LRQ EAIETHSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNIL-KKLKEEAK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  K +L  ++KEEA+
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of Sed0017952 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-120
Identity = 195/285 (68.42%), Postives = 240/285 (84.21%), Query Frame = 0

Query: 1   MLPFHSLSAAQAAVGRNLTVLETLWFDYSHDKSDYFLYCHNILFLFLIFSFIPIPLVFLE 60
           M+P+ ++  A  A+GRNLT  ET+WFDYS  KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  LFHVSGICSYKIQPKVRLSFSEIFRCYKDVMRMFFLVVGPLQLVSFPSVQMIGIRTGLPL 120
                    +KIQ KV+ S S++F+CYK+VM++F LVVG LQ+VS+PS+QM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LALGIPSFLGPAIVPGHMITLWSWIALRQIEAIETHSGYDFPWSLTKFIPFYGGADHHDY 240
           L LGIP+FLGPAI PGH++T W WI+LRQ EAIETHSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKNIL-KKLKEEAK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  K +L  ++KEEA+
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of Sed0017952 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-55
Identity = 111/250 (44.40%), Postives = 151/250 (60.40%), Query Frame = 0

Query: 33  SDYFLYCHNILFLF-LIFSFIPIPLVFLELFHVSGICSYKIQPKVRLSFSEIFRCYKDVM 92
           SD+ L C     L   +F    +P +FLE      +  YKIQ K     ++  +C   ++
Sbjct: 17  SDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNNTPAAQ-GKCITRLL 76

Query: 93  RMFFLVVGPLQLVSFPSVQMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCK 152
              F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++ED+  YW HR LH K
Sbjct: 77  LYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSK 136

Query: 153 WGYEKIHRVHHEYTAPIGFAAPYAHWAEVLALGIPSFLGPAIVPGHMITLWSWIALRQIE 212
           W Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H+ITLW W+ LR +E
Sbjct: 137 WLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLE 196

Query: 213 AIETHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRY 272
            +E H GY FPWSL+ F+P YGGAD HDYHH +      N++S F Y D+I+GTDKGYR 
Sbjct: 197 TVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRR 256

Query: 273 QKNILKKLKE 282
               LK LKE
Sbjct: 257 ----LKTLKE 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023552163.11.7e-16291.67methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo][more]
XP_022923513.13.8e-16291.33methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol m... [more]
XP_022965134.18.4e-16291.00methylsterol monooxygenase 1-2 [Cucurbita maxima][more]
XP_038876881.13.5e-16090.33methylsterol monooxygenase 1-2-like [Benincasa hispida][more]
KAG6596833.17.9e-16090.33Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q1EC692.5e-13276.04Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV... [more]
Q8L7W53.0e-13075.00Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV... [more]
F4JLZ67.0e-11968.42Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV... [more]
Q9AV398.6e-11767.00Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX... [more]
Q8VWZ82.1e-5444.40Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1E9U91.8e-16291.33methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=... [more]
A0A6J1HQ574.1e-16291.00methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 ... [more]
A0A6J1FUF26.5e-16090.33methylsterol monooxygenase 1-1-like OS=Cucurbita moschata OX=3662 GN=LOC11144822... [more]
A0A6J1L2B11.0e-15789.67methylsterol monooxygenase 1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111498458 ... [more]
A0A0A0L5P84.0e-15788.67Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
Match NameE-valueIdentityDescription
AT4G22756.11.8e-13376.04sterol C4-methyl oxidase 1-2 [more]
AT4G12110.12.2e-13175.00sterol-4alpha-methyl oxidase 1-1 [more]
AT4G22753.15.0e-12068.42sterol 4-alpha methyl oxidase 1-3 [more]
AT4G22753.25.0e-12068.42sterol 4-alpha methyl oxidase 1-3 [more]
AT1G07420.11.5e-5544.40sterol 4-alpha-methyl-oxidase 2-1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 131..265
e-value: 7.2E-29
score: 100.8
NoneNo IPR availablePANTHERPTHR11863:SF197METHYLSTEROL MONOOXYGENASE 1-2coord: 1..280
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..280

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0017952.1Sed0017952.1mRNA
Sed0017952.2Sed0017952.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity