Sed0017236 (gene) Chayote v1

Overview
NameSed0017236
Typegene
OrganismSechium edule (Chayote v1)
Descriptionisoamylase 1, chloroplastic
LocationLG12: 32043984 .. 32058021 (-)
RNA-Seq ExpressionSed0017236
SyntenySed0017236
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAAAAACCCAGAACTCACAATCCCAATACGCCAAATCAACGGTGGCACGTATGATCGTAGCTTTTCGTTTCTGTCCCATCTCTTACCCGAAAACAACAGCCGTGGACCCAATTTCTCTGTAAAGAACGGCCATGGCAGAAGCTACGAGCTCCATGTTCGCCCAATTTCGTCGTTAACGACACAGCACTGCTTCGATTGAGAGAGAGCCATACACTTTCACTGAGCTCTAACCTATTAGCGGCGATGAGGATAACAATTCTTTGAGCAAATGGAGATCGCTACTCGGAGTGCATCGCGGTATTCGTCTGCTTCGTTTCTACGTTCTTTGGATTTGAGTAGTTTGAAGCGCAATGCAGTTCGAATTCGGCGAAGAAGTGGTATCTGTTTTGATTTGGATGTGAATTCGAGGATGAGTGCGTCGGCGATCGGAGTGAGTAGTCGTGGTGCGGAGACGAGGACTTCTGTTGCGGTGGCGGAAGTGGCGGAGGCTTCTGATTTGAATCGGTTTGAAGTGTTGGAGGGTGTTCCGACGCCGTTCGGTGCTGCGGTTCGAGACGGCGGGGTTAATTTCGCTATCTATGCCGGCAATGCGGCTTCGGCTACTCTGTGTTTGATACATCTCTTTGATTTGCAGGAGGTATAATAGGAATCGATTCGAATTATTGCTTATACATTATCAACTATGTATCCCACAGGTTTCTTTATAGAAAGTATCTCATTCGATTGGTAGCTATTGCTATCGTACTCAATTAATGGTGACATGCACAAAAAATTTTGAGTATCGTTTTTTGGAGGAACTCTTGACACGATTAGTCGAGTTCGAGTATGTCAATGGAAAACTAATAGAGTAGCGTTTTTCTTTTATTTTTTGCATTTATTTTTCCTTTTTTTCATTTCTATATTGCAAATCAAGTAAAACTCCTCTACCATATGTGTAAAGGAGCTCTACTTTTTGGTAATGTGGCACCATTGGGTGTCACAAAAAATTATTTTACAAATGAAAATATCAAAATTTTATATAAATTTTTGTGGCGGTGCCACATAAGCAAAAAGTAGAGTTCCTCTATACATTCATGTGCACATGAGTTTTACTCTTACAAATCAATGCTAATGATGACCTTGTTGATAGAGATTTGAGGTTTCCAAGGCATACTCTCTCAAAGTTTTCATGTTTGAAATCTACGAGTGAGCTTAATTGTAAAATTTCTTTTTGTATTTGGTCGAGTATACAATGGGGTGAGCTTTGATTCCTGATTATATAAAAAATTATATTGCAAATCTCTTTTTTCTCCCCTTGAGGTAGTTATAGCTCAAGGCATTTCTATCTATAAATGAGATATTTGAATTAGTTATAGTCAATCCCAAGGGTGGTCGGTAGAGTCTTGAGGTTTCTAAGATAAGCTTCCTTAGAAGTCTCATATTCGAAAACCTCAAGGGAGCTTAATTGTAAAATTTATTTTTGTATCTTGATCCCGATTGCCTCGAGTATACAGTTGGGCGAAGCTTTGATTCACGATTATAAAAAAATAATAATAATTGTAATGCAATTTTTTTTTTGAGGTAGTTATAGTATAGCTCAAGAATTTTCTATATAAAAATGCGATCTTTGAATTAGTTTGCTCTAAATGATGGACCTCACTATTGCTGATGATTACCATTTACTTTGTGCAGAATAGAGTAGCCCAGGAGATCACCCTTGATCCCTTAACCAATAGAACTGGGGATGTTTGGCATGTATTTTTGAAGGGAGATTTTAACGAAATGCTCTATGGCTACAAATTTGATGGGAAATTTTCACCTCGGGAAGGATGCTACTTTGATCCTTCTCGGATTTTGTTAGATCCATATGCTAAAGTTCGTATTTTCATCATTTCCTTTTTGTCTTCAGTTTATGATGATAACTTTTTATCCCACATTCATTAGTTTCATTATTTCCCTATTTTCATCATTAGTTTATGATGACTTCCTATTTCTTTCCTCAGGCCGTTATTAGCAGAGGGGAATTTGGTGCCTTGGGACCCGATGACAACTGCTGGCCCCAAATGGCTGGCATGGTACCCACCGTTAATGACGAGGTAGAAAATGTAGTTCCTATTTGGCTAGCTTTGCCATTCTATGCTTCTTTTTTTTTTTGAAAGAAGGGTAACCACGCCCGTTCTTAAGCCAGACACTGGGAGACATCAAAGGAATGGAGACACGGGAGGTGTCTACAAATTTATATCACACGTGAGTCTCGAACTTTGGACCTTGAGGCCTGAGAGAAGCACACCTTCAAAGTCCAAGTTTCCAATCATCTGCGTCACCCCTGAGACAACCAATTAGCATAAGCTTACTAAACAAAAACAGAAATTTCCAAGAGAAAAAAGAACTTAGGTAACTAACCAATTGGTTTCATTGGAAATGTGACAAATGGTCTAAAAGTGATAATTATAAGTCTTGGTTGGTCCTCGAACCCTAGTTTCAACTGTACACTTAAGTAGCCTCCGGTGAAAGTTAGGAAACTTGTAAAATTAGTCAAATGTAGAAAATAATTACAAATATAGTCACTTTTTAAAAAAATTGGCAATTATAGTTATTGGTATTAATAATACATAAAATTGCATTAATAATACATAATACTCTATTAATAATACATAATACTGCATTAATAATACATAATAGTGTATTAATGAGTATTTACAATATAGTAGTAATGACTAGGTGAAAATATTTTAAAACTTGTCTACATTTGTAATTATTTTGTGAGAAGAGTCTATCCCATACAATTTCCCATAAATTTTTTGAAATACAATACCCTTCTATCTCATTGTAGTTTGTTTCTATGTTGAGGGCATGTAACATGTTTATATATCAATATATATTTGATGTGTTGTTTACAGTTTGATTGGGAAGGAGATTTACCTATGCAACACCCACAAAAAGAACTTATTATATATGAAATGCATGTACGGGGGTTCACCAGACACGAATCAAGTCAATCAGAATTTCCTGGTACCTATCGTGGTCTAGTGGACAAGTTAGATTACTTGAAGGTACGTTTTAGAGTGCTTCAATAACGAGCAAATTGTTTATACCGTTTCTGCATGCCTTTTTCTTTTGGATATCCGTGACTGACCGAGCTAGTTTGCGCGCACCTCGACTAATTTCACGGAAATTCTTAGGTAATAGTCATCATGGATTGAACTCATCACCTCTTAGTTTCCCAAGACCCTCTTTGACCACTAGGCCACACCATGATGGTTTTGCATGCCTTTTTCTCTAAATCAATTCATGTATCTCTTATAACCATCATGTAGCGTCCGAGGCCCAAGAATCGGACCAGGGGTGTTACACATCATGAATTAGCCTAGTGGTTAGATAAAGGTCTTGAGAGTGGAAATAAATTCAATTCATCTGGTCATCTATATAGGAATTAATTTCCTATGAGTTACCTTGACAACCAAATGTAGTAGGGTCAGGCGGTTGTCCCATAAGTTGGCCCAAACACTTGCAGATAAAAAAATAATTTAATGTATCTCTTATTTCAGAAAATGCAAATGTTAATGTTAGCGGAGAGCAACATTTCTTTTTATTTTGCTTTTGATGTTCCCAACTTAAACATAAAATTATTTTGATCTTATATAGGGGATTTGCATCATCTAGAACTCTTGATTGTATGCGTAAAAAATTCTTGGTTTTGTAGTATAAAAGTAGGAGGGAATGAGCTAAATCTATGGTGGCCACCTTTGACTTACCTAGGATTTAATATCTTATGTGTTGTAGTAGTGTAAGACAGTTGCTCGATGAAGTTAATCGAGATGCGTGCAAGCTGGCCAAGACACTTGCCAATATAAAATATTGTAGTATAAAAGTATGTATAACAAATGTCGTCTACAAACAATTCCGTTACTATTTTACTTCTATGTTTAGCAAAATAATAAGCTAATAAAAAGTCATGTAATTGTTGGTTATCAAATTCCCGAACCACTGATAAAGCAAGGAAAGTGATTGATTCAATTAAAAATAGTACATGCCACTAGCTTATAAAAACTGTTTCAGTGAAAGACAAATAGGATATGGTAAGGAAAAATTATTCCCTTGAGGTAATGGAGTATTTCCTTTATTTGACCAAATAGTTGTTGAGCCTCTTTGTTTTCACTTGGTTATGAAACTGATGCGAGTCCTTTCTTTAGTTACACTCACAGCCCATGCATCAGATTTGTGCAAGGACAGTGAATCAGCAAGGGTCTGTTTCTGTATGGAACACAAAACTCTGACTGCTGAGGAAAATAAATGGATATTTTAAAACTTCTTTTGAAGTTATTTTTGTTTAAATATTTGTAGTAGGGTGAGACAATTGTCTTATGATTCGAAGTGCATAAGTTAGTCCAAACACTCAAAAATATAAAAATGTCTCGTAGTAAAGTATTGCATTTTGTTTCTGATGAGGCTGCTCGTCACTCCATTCAACTTTATTGACTTTGAACCTAATAATTTCTTCGTTTATTATTTATTTTGTTTCTAAAATGCAAATCAGAATGCTTTCTCTGGCTCTGCCCTTTCATTTCCTCAGACGTTACTAGCACTTATCCTATTCTTCTGTCCTCTTCCTAATGGCTCAACTCTTTTTTAATTATTTTTCCTTTCAGGAACTTGGGATCAACTGTATAGAGTTAATGCCTTGCCATGAATTCAACGAGTTGGAATATTACAGCTACAACTCTGTCTTGGGTGACTATAAGTACTATTTGATTCCTTAATTTGGAGAACTTTTTTATTATTTCTATAGTTTAACGTTGTCTTAACATTTTCCTCAGAAAAAAAAGTGCTGAGATCCTTGCGTTTTAATTATGATTTAATAGTTTACACATAGAGTATTATTTTGTAAGAGTACAAGTTACCACTCTTGAAGATTTGTTATATATAGTGATAAAATACCAAACTTTCATTGAGATAAAAACGATAGAATACACCGGCATACAAAAAGAAAATCCCACAAAAAAGTAAAGATGAAGCCAACAAAAAATAGCTTCCAATCCAACAAAATCTGACCTAACGAATAATTAAAAGGGAAACACAGTCATCAAGGTCCAAAAGGAAGATTTCCCCAAGTTTTGTTATATTGTAATGACTTTGTTTTGTTTTAATTGGTGACCGGCAACAGTAGGCTGCATACTCTTTATACCCATAATCCAATATCATTCTTGACGTTTTCTTTTTTGTCATTGAGTTATATCATGAAGTAAATGTTTTTGTCTAGGATGAATTTTTGGGGATATTCAACAGTCGGTTTCTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGCCAAGATGCTATAAATGAATTCAAGCTCCTCGTTAAAGAAGCTCATAAACGTGGAATTGAGGTGGAAGAATGAAATACTTGGTTACTTTTGAAATATGGCTTACTTTTCATCTTTTAATTTAGTTTTTTTTTTATAATCAGGTGATCATGGATGTAGTTTTTAATCACACTGCTGAAGGGAATGAAAATGGTCCGATTTTTTCTTTCAGAGGTGTTGATAATAACGTGTATTACATGCTTGCACCAATGGTATGCATCTGTAAGATTAATAATTTTCCTTTCTTGTATTTTATTCAGAGGTGAATAAAACAGCATCGATATGATTTCTCAGTTTCTTATTTGAAAAGAGCATTAATAACAGATGGAAGAATCTGTTTCAAAAATAAAATAAAAGATGAAAGAATGGTCTTTGATTTTTCTTAATTTAGCTTTCTTCGAAGTTTTTGTTATTTTTATCTATATATGATATTTTGATAGTGCTTCAACTGCCTGAAACATCTATAGGCATATTTTTACCATAAATCCCTCTAATGCTGTGTTATTCGTGGAATGAAAATCTCTAATTTCCTTCCATCTAATATGTGGACCCAAGCATGCCACTGCTGTTGTAAAGAAAAGAAAAGACATCTTTAGACTTGATAATTGCTGAAATAAAGGGGTGAAGGGAGAAATATTTAGAAACTGGCGGTTGTTTTTAAATCTTTATATATGTTTAAGACTGTGATGCGTGCATTCAACTTTTGGAGCCTGGGCATACAGTATATCGCTTAATTACGCTGGATGGTTGTGTTGCTTTAAATTTTAACCGGTAGGGCAATATTACCCATGTGCTAAATTTTAAGTGGAGGATAAAATCAATGGGCATGGTTTGTTCAGTCGTTTCTCTCTCCTTTTTTTTCCTTACCCAATACTTACATGTTGAAAATTCTATTTATCTCTTTTATTTTAGTTATTTATATAAGCTTAACCATGGTTTTTCAGGGAGAGTTTTATAATTATTCGGGATGTGGGAATACACTTAACTGCAATCATCCAGCTGTACGTAGATTTATTGTGGACTGCTTGAGGTAGATATATCGTAGTTGTGGATACTGTATTTTCGGGTAGATATATCATAGTAGTGGATACTGTATTTTCGGCATATGTTAGTTTCGATTTGTAGAAACACATGAAAAGTTGAAGAGGTGAATGAAAGATTAGATTTCTACTTGTAGAAAGAATTACGGAGAAGCTGCATAATAAATCAAATATAAACCAAAGTATAAATTGGAAAAACAAATAAGGATAAAAACATGATTAGAGTGAGTGAGAGAGAGAGAGAAGCCTGAAGCCCCACTGTAAAAGAGGTATGGGCATTGTTTTCCTATAAGAAAATAAATGGGGTTTGTACGAGGAATCAAATTGAAGATTAAAGAGCTTCAATTTTTTAGAAAAAGAGACCGAGGAACAACTACTTGCAGATAAATATGTAGGGACATGTATTAAAGGCCACACCTAATTCCAAGGATTCTGGAAGCCGTCTAAGAATTTCTTCTACTTCCCTCAAACCAGGCATTCTCAAAAATAGAAGAAGAAAAAAACGCTACAAACCAAAGAGCCTCTCCCTCGTCTCACTAACACTAGATAACTCAACTGACATTCATGTACTATGACAAGAGGTCACTAGTTCAAATCCCCCCTCACACTTCATAAAAAGAAAATCCAACATCAAACATACAATTTATTACAAGTCAAACACACTTCAGAAGTCTAAAAAAATTATCCCAAGTTGTATTCGGCTCACAAGAGCAATAGTTCAACTGACATAAGTATGTGTCCATGACCAAGAGTCACGAGTTTGAATCTCTCATCCTCAATTATTGTTTTAAATATTGGCAGCCCCAGAAAACCAAAACCAGACTTGCAAAAATGAAAAATATACTCAAGTGACAAAACTCACCAGCCCCAATAACATAGAAGAATGCCTTTGAAATAGGATGTTATACATGTCCAGCTTTCTAAATTCGAAAATATTCTGCTATCTGTCAGATTATTGTCTCTTACTGTTGTTTTTTGTCTATCGTGTCTTTTGGCTCTACCTTGTGGACATCTCCTCTGTTCCTCTCTCATGCAAGGAGCGTTGAAATGTCAAGCTAACGAATTTAATCACTCTCATTTTTATAAGGTTTTTCTCCTTTGTAATTTTGTTCCTCAAGAAATATATCTAATGACCTATGTACAAGTGCACATGTAACATGTTGTACTTGGACTTGACCTTTTCCTATGATTGCGCTTGCACATCCAGCATTTTTAGAATAATTTATTTAGTTTTTGAGAAGCCCGACTTTGGCCACAGATATTGGGTGACAGAAATGCACGTGGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGGTTATTGGCTGAACTTTCTGTCTGTTGCTTGATATCTGTATGATTAATTGTCTCATTATTCATCCTTTTCATCTTTTGTGGGCAGCCTCTGGGATCCAGTAAATGTGTACGGAAGTCCAATAGAAGGTGACACACTCACAACTGGTTCCCCTCTGGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAATACTTCAAGGAGTGAAGGTCTTTACTCTAAATTATTTTCATGTCTGCTTTGGGGGCAATTATAAACCGTAAATAGTTTTATTGTTTTTTCTAATCTGCCACAAATTATCTATTTGAATTATAATTTTGATTAACTCTACTAGCTCTAGCTCATTAGAGATTATGAAATAGGCAGGTTGCTGTTGCTGCTTCTTCGATAAGATTTGACTTCTTAATTCTTCCTTCAATAGTTTGGTATTTTATTAGTATTGATCCTTTTAGAAGTGGAGTGAATTATTCAACAATAAGACCATCATCCATATTCTTACACCTATTCCAACGTACCACACGAGGAAAGGATGACTTATATTTGTGAGTTAACAATATCCTATCGATGATGATATTTTGTTGGGGGTAATATCATCCCATTCTTACCTTGTCAATCATCTCTTGGAATAAGAGATATTGTTTTTTTTCTAGAATTTGATACACTAGTTTTTAGGTGATTTTTGTCATTTGTTGGAGACAAGTATCATATCCCACCACATTTTTGGACGTTTTGGTTGAAGTGGATAAAGATACAATTAGAAAAAGGAGATGATGCATTTCTTTCGAAGAAAATTATTAGGATAGGACATGGTCCATTTGTACCTAAATCCTTCCCCGTTTCTCCAAGTCAATAATTGCAAAACACGCGTTTGGAGTAAGCACCTAAACCTTTTTATCTGCTAACATTTTCATTTTCAGAAATTGACATGCGCCAAGTTTGTCAACCATTATTTGGATAATTTTCTCTTTGAAGATTTATTAGGCACCGTTTGATTAATTATTCGGTTTCTTTTCATGAATTGACACTCCAGTTTAAATGTTTCAGGAAGTTAAACTGGAGTGTGAATTAGTCTTGTTAATTATCCGTTTGATTCTATTTGTTCCTTTTGTTGCTACCATTATGACTTAGTCTTAATCTTTTTTCTCTTTGAAATAGCTCATTGCAGAAGCATGGGATGCCGGAGGGCTCTACCAAGTTGGTACATTCCCTCACTGGGGTATTTGGTCCGAATGGAACGGGAAGGTTTGTGTAGTTCTTTTTTTCTTCTTTTTTTTACTATCATAGGTTGACCTTGTGGTCAATAGACTCTTAGGGAAATAAGAGGGAATAAGTTCAATTCAGGTCAATAGAGTCTTAGGAAAATAAGAGTGAATAAGTTCAATTCAGGTCAATAGGGTCTTAGGAAAATAAGAGGGAATAAATTCAATCCATGGTGGCCATTTGGACTATCCAATGCCTCAACATTTCCATGAAGCTCACGAGTCAGATTCTTCAAGCCAATGGAGGTATGAAATAAGAGAGATTGTTGTAAGGAGAAGTAGGAGAAGGATGAGTTTAGAAAGTACTGAGAGGTCTAATAAACCAAGTAAACAACCATTAAACTGAAAGTTATAAGTAAAAGAGTGCCACCTTACCTAAATATGAAAATAAGAATAACTGGAATTTGGTGGATCTTCAATTATCCAAGTAACTTTAAGATTTGTCAGAAGTCTTGATGATTCTCCATGTGCTATAAGAACCTCCAGCTCCGTATTTGCATAATTTGATAAAAAAAAAGTCTTGTTTCTTATAAAAAATGAAGTTCGATCATAACTTTTTATTTTTGGTCAATTTCAGTTCCGAGACAATGTACGTCAGTTCATCAAAGGCACAGATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAACCTTTACCAGGTTATTTAGAGTTCTCTTCCTTCTTTTTGGTCACATATGTCACTTTTCTACAGATAGTTTAGTCCGACCCTGTCAACATTTTGTAAGAAGGATAACAGAAATAGCACAATACCTTTGGATATATTTTTTAAATTTGAACAAAGAACTGAACGATCATTATTAAGCCCCATGACAGGGAAGAAATGCTTTGGCGCAAGCGCAAGGCTTCCTATGGGGCATGAATATTAGAAATCAGAATAATGTCTACATTGCTAGAGAGGCGGGCTATGAACGAGTAAAAGCTCTGTGAGCTGGGCATTGTAAATGTGCGGCATACTGCCTGGTTTCAAATTGATTTTTCATTTTGAAGTGTTTCTAAGGCTCCGCTTGATAACCATTTTGTTTTTAGTTTTTGGAATTTAAGCTTACAACAACTATCATACCATGTGTTTTCTTCTACATGGATATCTACATCTTACCTATATTTGTAGAAACTAAACCAAAATTTGGAAACAAAAAAAAGTAGCTTCCATAAGTTTGTTTTTGTTTTCGAAATTTGGTTAGAATTCACATACTTTCTTAAGGAAAGTTAGATATATTGTAAGAAAGATAGAGCACATCGTAGGAAATATGAGTAAAAATAAGCTTAAATTTCAAAAACAAAAAACAAAAAAGCAAATGGTTATCAAACGGGGCCTAAACTTTCTAGTTCTTTGTATGTATGTATATACATGTTAAATATTGCTCACGATTACACAAGTATGTAAAAGTTACTTATGTATATGGATTTGAATATGAAAATTACGTTTTTTTTAATAGATTTATAGACAGTCTTTCTACACCCGCCAAAATGCTGTAAATTTTATGTCGACTTTACCAATTACAGTTTGGTTGAACTTTTGGCTAATTGGAGGGTTCTTTTCCTCCTATTTTATATTTCATCATGTTAATGGAAAATTGGAAATATCTTGGTTCCTACCCCCCAAGAAATGATGAATTATAGTCTAATTAAACAATAGTCATAGGAACAAGTTTTCTCTTTTTCCTTGCTTCGTATTGTTTATTTGAAGTCTAAACTTAGTTCCATCTCTTGTACCATTAGGCCTAGTGTTGGATAGGGCGTTTGCATGTTGAATAGAATATCAAACAGTGGATTCACATTTACCTATTGACTCAAGTATCTAATGATGTTTTTACAGGAAGGAGGAAGAAAACCATGGTGTAGTATCAACTTTATTTGTGCACACGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACATAACTTAGCAAATGGTGAAGACAACAACGACGGTGAAAATCATAATAATAGCTGGAACTGTGGACAGGTATGCAATCAGTTGTATGCATCTCATAAGATTATCGTTGAAGATAAGATAATTGGACCGTATAAACAATGGACACTTGGTGGTATTTCTTTTCATTTCAAGCATTTTCCAACGGTTGAAAGGCAAATATAAAGTAGATAGGGAGTCTTGAGATTTTTTTTTTCTTTTAAAATCGAGAAATCGGAGTTTTGCTCCACTATATCCGAGATAACCACGTCCGTCCCTAGGCCGGGAGACATCAAAGGAATGGAAACACGGGACGAGTCTACAAATAAATTTTACAGGCGAGTCTCAAACTTGGGACTTTGAGGGGAGCGCACCTTCAAAATTAAGTTTTCAACCACCATGCCACTCCATGGAGACATGGGGTGTTGAGATTTTATTAGACAATCAAAGAAATTACGCGAGGCTTTTGAAATAAATGAAAGAAAACAAATTAGGCATAATCACCATGTCCCTTAAATTTTGAAACCATGCATACATTAGTTATTGCGCATACAAGAGTTTGGTTTGATGGTTTTTTTTTTTTGAAACCGGGAAATTTGAGCTCTCTCTCCATTATACCCGGACCTGGGAGACAACTAGGGAGAATTTGGTTCAATAGTTAAAGCTCAATTCCACTAATGGCTTCAAAATCTTGGCGTAGATACGAAAGATCTTGCTGCTTACCATCGTTGCATATTATTATTATCTTATTGCAACATGATTTTTTCCCTCTTAAATTTGCATGTTTCATATATTTTACAGGAGGGTGAGTTTGTAAGCATCTCTGTAAAGAAATTGAGAAAAAGACAGATGCGAAATTTTTTTGTCTGCCTCATGGTTTCCCAAGTAAGATTCACTCCTATCCTTTAATAAATTATATGTTAATTCTTTGCATTGCCAACAAGAGCGTAACTCAACCGGCATAAGTGTGTCCATGACCAGTCGTGGGTTCAAATCCCCAATGGTTGTTTAACTGAAAAAAATATGTTAATTCTTTACGTGGATTAAACTTGCACAGAAGTTTCTTATCCACTGAGCGCTCATTCTTGTAATATGATATGATTACATGCCACAGTACAGCATATTTCACCATTAGTAACATTTATTTCATCATAGTTGCTCTCTAGTATAAGAGAATCTACCTATCCAATTTGTCAATTTAATTCTTATGAAATTAGTTTTTAAGCTCTATTCTAACTGCTTTTATCTGACCAGGGCGTCCCAATGATATGTATGGGTGATGAATATGGACATGCAAAACGGGGGAACAACAATACATACTGCCATGATAACTATGTAGTTTCTTCATCTTGCTATTCTCTGACCTATGTTGTTGAGCTGAAATTCAATTCTGTTGACCAGAACTGCTAACTTTGCTTCAGATAAATTATTTTCGCTGGGATACGATGGAAGAGTCATCTTCAGACTTCCATAGATTTTGCAGACTTATGACCACGTTCCGTTCGTAAGTGTCGCCCTCTCCGAATTGATTTTGTTTCATGTAGTTTGGTGTTTTAAATGACTATTAACCTGTCTCGATGCCTTTTTTATATGACTTGCAGAGAATGCGAGTCGCTTGGCTTAAATGATTTCCCAACAGCTGAGAGATTGCAATGGCATGGCCATACTCCTAGAAACCCAGACTGGTCTGAAGCAAGCCGTTTTGTGGCCTTTACTCTGGTATGTTTCCTTTCCAAATAACATTTGCTCATGCAAACCATATTTCTTCCTCTGTTTTAACAATTTGGCTTGTTGTCAAAGACTCAAAATTATATTTAGGTTAAATTACAAGTTCAGTGAACCTGAATTTCTTGAAATTTGGAAGTTTAAGAATCGATTAGATGCTTTTTAGGGTGTGTTTGGACAGATGGCTTTGTCTCCTGGGTATGTGTCTTGAGACGGGTGCGGGTATATGGGAGTGAAGCTCCGATTTTCGGAAAAGAAAAAAACAAATGAAAAAAAATGAGGGGTTTTGAGTGCTTATGAAGGGTTTCTTGAAGATAAAACTTTATTTGGATTGATGGGTTAGGAGTAGTTGTGGTAGAAAGTAGAAACCATTCTTTAGATAGAGGTGTTGTGTGCGATTGTTCCAGAAAACCATTGTTTGGATAGATGGGTTATGACTTATGAGTTGTTGTGGCAGAAACCCTCGTTTGGATAAAGAGGTTATGGCTCATGAGTGGTTGTGGCAAAAAACTCACTTGGATTGCGGGGTTATAAGTGGTTGAAAGTAGGAGTGATATTGTATCTAGAGGGTTTAAGAATGTTTATAGAGGGTTTTATGAAGGTTTTTATGATGGATTTTTGGAGCTGCTTCATCCAAACATGGGGTTTGGTCAATTCTGAACAAACTCTCACAAACTTTCTATCCAAACACACTTTTAAGGATCTATTTAACACAAATATTAAGTTTTGGGACTCAATCAGTAATATTATTTAACATCATATTTATACCATCTTTATGGCCTTCGACTCTTACTTTTCGAAGATCGATACCTTAACATATCACATATTGTTGTTAAATTTGTTGAGATCATGTCAGTTGTTGTACACTCAAAGCACTGATTCCTGTTATGAGATGACTTGTACCCAGGTTGATTCAGTAAAAGGAGAAATTTACATAGCCTTCAATGCCAGTCATTTGCCCGTCACCGTGGCGTTGCCCGAGAGGCCCGGATGCAGATGGGAACCGCTGGTGGACACAGGTAAAGTTTCGCCATTCGACTTCCTCGCAAGTGACCTTCCGGAAAGAGATTTGGCAATCCAACAGTATGCTCACTTCCTTGACACCAATCTTTATCCCATGCTTAGTTATTCTTCCATCATTCTTTTACTCTCTCCAGAAAAACATGCATCATTTTGATCATCTTCATATCACATTCCCCTTAATAATTCTTCTTCTCAATCATATGTGAATATTTCATTTATAATAAATACACAGTAGGATGTCTGTGATATTTATTTCTATTTTATATGACCTTCATCCTTAAACTATTGCTTTTATTTGACTAAAATTTTGTTCAGATTTAATTTAATTCACTGGATTGATTGAG

mRNA sequence

GAAAAAAAAACCCAGAACTCACAATCCCAATACGCCAAATCAACGGTGGCACGTATGATCGTAGCTTTTCGTTTCTGTCCCATCTCTTACCCGAAAACAACAGCCGTGGACCCAATTTCTCTGTAAAGAACGGCCATGGCAGAAGCTACGAGCTCCATGTTCGCCCAATTTCGTCGTTAACGACACAGCACTGCTTCGATTGAGAGAGAGCCATACACTTTCACTGAGCTCTAACCTATTAGCGGCGATGAGGATAACAATTCTTTGAGCAAATGGAGATCGCTACTCGGAGTGCATCGCGGTATTCGTCTGCTTCGTTTCTACGTTCTTTGGATTTGAGTAGTTTGAAGCGCAATGCAGTTCGAATTCGGCGAAGAAGTGGTATCTGTTTTGATTTGGATGTGAATTCGAGGATGAGTGCGTCGGCGATCGGAGTGAGTAGTCGTGGTGCGGAGACGAGGACTTCTGTTGCGGTGGCGGAAGTGGCGGAGGCTTCTGATTTGAATCGGTTTGAAGTGTTGGAGGGTGTTCCGACGCCGTTCGGTGCTGCGGTTCGAGACGGCGGGGTTAATTTCGCTATCTATGCCGGCAATGCGGCTTCGGCTACTCTGTGTTTGATACATCTCTTTGATTTGCAGGAGAATAGAGTAGCCCAGGAGATCACCCTTGATCCCTTAACCAATAGAACTGGGGATGTTTGGCATGTATTTTTGAAGGGAGATTTTAACGAAATGCTCTATGGCTACAAATTTGATGGGAAATTTTCACCTCGGGAAGGATGCTACTTTGATCCTTCTCGGATTTTGTTAGATCCATATGCTAAAGCCGTTATTAGCAGAGGGGAATTTGGTGCCTTGGGACCCGATGACAACTGCTGGCCCCAAATGGCTGGCATGGTACCCACCGTTAATGACGAGTTTGATTGGGAAGGAGATTTACCTATGCAACACCCACAAAAAGAACTTATTATATATGAAATGCATGTACGGGGGTTCACCAGACACGAATCAAGTCAATCAGAATTTCCTGGTACCTATCGTGGTCTAGTGGACAAGTTAGATTACTTGAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCTTGCCATGAATTCAACGAGTTGGAATATTACAGCTACAACTCTGTCTTGGGTGACTATAAGATGAATTTTTGGGGATATTCAACAGTCGGTTTCTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGCCAAGATGCTATAAATGAATTCAAGCTCCTCGTTAAAGAAGCTCATAAACGTGGAATTGAGGTGATCATGGATGTAGTTTTTAATCACACTGCTGAAGGGAATGAAAATGGTCCGATTTTTTCTTTCAGAGGTGTTGATAATAACGTGTATTACATGCTTGCACCAATGGGAGAGTTTTATAATTATTCGGGATGTGGGAATACACTTAACTGCAATCATCCAGCTGTACGTAGATTTATTGTGGACTGCTTGAGATATTGGGTGACAGAAATGCACGTGGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGCCTCTGGGATCCAGTAAATGTGTACGGAAGTCCAATAGAAGGTGACACACTCACAACTGGTTCCCCTCTGGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAATACTTCAAGGAGTGAAGCTCATTGCAGAAGCATGGGATGCCGGAGGGCTCTACCAAGTTGGTACATTCCCTCACTGGGGTATTTGGTCCGAATGGAACGGGAAGTTCCGAGACAATGTACGTCAGTTCATCAAAGGCACAGATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAACCTTTACCAGGAAGGAGGAAGAAAACCATGGTGTAGTATCAACTTTATTTGTGCACACGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACATAACTTAGCAAATGGTGAAGACAACAACGACGGTGAAAATCATAATAATAGCTGGAACTGTGGACAGGAGGGTGAGTTTGTAAGCATCTCTGTAAAGAAATTGAGAAAAAGACAGATGCGAAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGCGTCCCAATGATATGTATGGGTGATGAATATGGACATGCAAAACGGGGGAACAACAATACATACTGCCATGATAACTATATAAATTATTTTCGCTGGGATACGATGGAAGAGTCATCTTCAGACTTCCATAGATTTTGCAGACTTATGACCACGTTCCGTTCAGAATGCGAGTCGCTTGGCTTAAATGATTTCCCAACAGCTGAGAGATTGCAATGGCATGGCCATACTCCTAGAAACCCAGACTGGTCTGAAGCAAGCCGTTTTGTGGCCTTTACTCTGGTTGATTCAGTAAAAGGAGAAATTTACATAGCCTTCAATGCCAGTCATTTGCCCGTCACCGTGGCGTTGCCCGAGAGGCCCGGATGCAGATGGGAACCGCTGGTGGACACAGGTAAAGTTTCGCCATTCGACTTCCTCGCAAGTGACCTTCCGGAAAGAGATTTGGCAATCCAACAGTATGCTCACTTCCTTGACACCAATCTTTATCCCATGCTTAGTTATTCTTCCATCATTCTTTTACTCTCTCCAGAAAAACATGCATCATTTTGATCATCTTCATATCACATTCCCCTTAATAATTCTTCTTCTCAATCATATGTGAATATTTCATTTATAATAAATACACAGTAGGATGTCTGTGATATTTATTTCTATTTTATATGACCTTCATCCTTAAACTATTGCTTTTATTTGACTAAAATTTTGTTCAGATTTAATTTAATTCACTGGATTGATTGAG

Coding sequence (CDS)

ATGGAGATCGCTACTCGGAGTGCATCGCGGTATTCGTCTGCTTCGTTTCTACGTTCTTTGGATTTGAGTAGTTTGAAGCGCAATGCAGTTCGAATTCGGCGAAGAAGTGGTATCTGTTTTGATTTGGATGTGAATTCGAGGATGAGTGCGTCGGCGATCGGAGTGAGTAGTCGTGGTGCGGAGACGAGGACTTCTGTTGCGGTGGCGGAAGTGGCGGAGGCTTCTGATTTGAATCGGTTTGAAGTGTTGGAGGGTGTTCCGACGCCGTTCGGTGCTGCGGTTCGAGACGGCGGGGTTAATTTCGCTATCTATGCCGGCAATGCGGCTTCGGCTACTCTGTGTTTGATACATCTCTTTGATTTGCAGGAGAATAGAGTAGCCCAGGAGATCACCCTTGATCCCTTAACCAATAGAACTGGGGATGTTTGGCATGTATTTTTGAAGGGAGATTTTAACGAAATGCTCTATGGCTACAAATTTGATGGGAAATTTTCACCTCGGGAAGGATGCTACTTTGATCCTTCTCGGATTTTGTTAGATCCATATGCTAAAGCCGTTATTAGCAGAGGGGAATTTGGTGCCTTGGGACCCGATGACAACTGCTGGCCCCAAATGGCTGGCATGGTACCCACCGTTAATGACGAGTTTGATTGGGAAGGAGATTTACCTATGCAACACCCACAAAAAGAACTTATTATATATGAAATGCATGTACGGGGGTTCACCAGACACGAATCAAGTCAATCAGAATTTCCTGGTACCTATCGTGGTCTAGTGGACAAGTTAGATTACTTGAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCTTGCCATGAATTCAACGAGTTGGAATATTACAGCTACAACTCTGTCTTGGGTGACTATAAGATGAATTTTTGGGGATATTCAACAGTCGGTTTCTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGCCAAGATGCTATAAATGAATTCAAGCTCCTCGTTAAAGAAGCTCATAAACGTGGAATTGAGGTGATCATGGATGTAGTTTTTAATCACACTGCTGAAGGGAATGAAAATGGTCCGATTTTTTCTTTCAGAGGTGTTGATAATAACGTGTATTACATGCTTGCACCAATGGGAGAGTTTTATAATTATTCGGGATGTGGGAATACACTTAACTGCAATCATCCAGCTGTACGTAGATTTATTGTGGACTGCTTGAGATATTGGGTGACAGAAATGCACGTGGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGCCTCTGGGATCCAGTAAATGTGTACGGAAGTCCAATAGAAGGTGACACACTCACAACTGGTTCCCCTCTGGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAATACTTCAAGGAGTGAAGCTCATTGCAGAAGCATGGGATGCCGGAGGGCTCTACCAAGTTGGTACATTCCCTCACTGGGGTATTTGGTCCGAATGGAACGGGAAGTTCCGAGACAATGTACGTCAGTTCATCAAAGGCACAGATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAACCTTTACCAGGAAGGAGGAAGAAAACCATGGTGTAGTATCAACTTTATTTGTGCACACGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACATAACTTAGCAAATGGTGAAGACAACAACGACGGTGAAAATCATAATAATAGCTGGAACTGTGGACAGGAGGGTGAGTTTGTAAGCATCTCTGTAAAGAAATTGAGAAAAAGACAGATGCGAAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGCGTCCCAATGATATGTATGGGTGATGAATATGGACATGCAAAACGGGGGAACAACAATACATACTGCCATGATAACTATATAAATTATTTTCGCTGGGATACGATGGAAGAGTCATCTTCAGACTTCCATAGATTTTGCAGACTTATGACCACGTTCCGTTCAGAATGCGAGTCGCTTGGCTTAAATGATTTCCCAACAGCTGAGAGATTGCAATGGCATGGCCATACTCCTAGAAACCCAGACTGGTCTGAAGCAAGCCGTTTTGTGGCCTTTACTCTGGTTGATTCAGTAAAAGGAGAAATTTACATAGCCTTCAATGCCAGTCATTTGCCCGTCACCGTGGCGTTGCCCGAGAGGCCCGGATGCAGATGGGAACCGCTGGTGGACACAGGTAAAGTTTCGCCATTCGACTTCCTCGCAAGTGACCTTCCGGAAAGAGATTTGGCAATCCAACAGTATGCTCACTTCCTTGACACCAATCTTTATCCCATGCTTAGTTATTCTTCCATCATTCTTTTACTCTCTCCAGAAAAACATGCATCATTTTGA

Protein sequence

MEIATRSASRYSSASFLRSLDLSSLKRNAVRIRRRSGICFDLDVNSRMSASAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQYAHFLDTNLYPMLSYSSIILLLSPEKHASF
Homology
[Close]
BLAST of Sed0017236 vs. NCBI nr
Match: XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 712/808 (88.12%), Postives = 741/808 (91.71%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S K N + IRRRS I  DL V   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV VAE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPL NRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS FPGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFKLL+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPERPG +WEPLVDTGK+SPFDFL SDL ERD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLLSPEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAS 808

[Close]
BLAST of Sed0017236 vs. NCBI nr
Match: KAG7021674.1 (Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1492.6 bits (3863), Expect = 0.0e+00
Identity = 711/808 (88.00%), Postives = 741/808 (91.71%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S K N + IRRRS I  DLDV   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV VAE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPL NRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS FPGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFK L+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPERPG +WEPLVDTGK+SPFDFL SDL +RD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEKRDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLLSPEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAS 808

[Close]
BLAST of Sed0017236 vs. NCBI nr
Match: XP_023531773.1 (isoamylase 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1491.1 bits (3859), Expect = 0.0e+00
Identity = 710/808 (87.87%), Postives = 742/808 (91.83%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S K N + IRRRS I  DLDV   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSR AE RTSV VAE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRRAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPLTNRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS FPGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           S+LGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFKLL+KE+HKRGIEVIMDVV
Sbjct: 301 SLLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKESHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLR+RQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRERQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPERPG +WEPLVDTGK+SPFDFL SDL ERD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLLSPEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAS 808

[Close]
BLAST of Sed0017236 vs. NCBI nr
Match: XP_022973965.1 (isoamylase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1488.4 bits (3852), Expect = 0.0e+00
Identity = 711/808 (88.00%), Postives = 740/808 (91.58%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S KRN + IRRRS I  DLDV   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV +AE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPLTNRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS  PGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFKLL+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYF WD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPER G RWEPLVDTGK+SPFDFL SDL ERD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLL PEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLFPEKHAS 808

[Close]
BLAST of Sed0017236 vs. NCBI nr
Match: KAG6587707.1 (Isoamylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 710/820 (86.59%), Postives = 741/820 (90.37%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S K N + IRRRS I  DLDV   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV VAE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPL NRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS FPGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFK L+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAP------------MGEFYNYSGCGNTLNCNHP 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAP            +GEFYNYSGCGNT NCNHP
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPILLTLDGCFKFKLGEFYNYSGCGNTFNCNHP 420

Query: 421 AVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGN 480
           AVRRFIVDCLRYWV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGN
Sbjct: 421 AVRRFIVDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGN 480

Query: 481 PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDG 540
           PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDG
Sbjct: 481 PPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDG 540

Query: 541 FSGAFAECLCGSPNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE 600
           FSGAFAECLCGSPNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE
Sbjct: 541 FSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGE 600

Query: 601 NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTY 660
           NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTY
Sbjct: 601 NHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTY 660

Query: 661 CHDNYINYFRWDTMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPD 720
           CHDNYINYFRWD MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPD
Sbjct: 661 CHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPD 720

Query: 721 WSEASRFVAFTLVDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLAS 780
           WSEASRFVAFTLVDSVK EIYIAFNASHLPVTV LPERPG +WEPLVDTGK+SPFDFL S
Sbjct: 721 WSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTS 780

Query: 781 DLPERDLAIQQYAHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           DL +RD+AIQQYAHFLD NLYPMLSYSSIILLLSPEKHAS
Sbjct: 781 DLEKRDVAIQQYAHFLDANLYPMLSYSSIILLLSPEKHAS 820

[Close]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 566/743 (76.18%), Postives = 642/743 (86.41%), Query Frame = 0 

Query: 53  IGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASAT 112
           I +S++   +  +  +A V +    +RF + +G+P+PFG  VRD GVNF++Y+ N+ SAT
Sbjct: 40  ISISAKDRRSNEAENIAVVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSAT 99

Query: 113 LCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGCYF 172
           +CLI L DL++N+V +EI LDP  NRTG VWHVFL+GDF +MLYGY+FDGKFSP EG Y+
Sbjct: 100 ICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYY 159

Query: 173 DPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELI 232
           D S ILLDPYAKA+ISR EFG LGPDDNCWPQMA MVPT  +EFDWEGD+ ++ PQK+L+
Sbjct: 160 DSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLV 219

Query: 233 IYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSV 292
           IYEMHVRGFTRHESS+ EFPGTY+G+ +KLD+LKELGINCIELMPCHEFNELEYYSYN++
Sbjct: 220 IYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTI 279

Query: 293 LGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFN 352
           LGD+++NFWGYST+GFFSPMIRY+SA   N    AINEFK+LVKEAHKRGIEVIMDVV N
Sbjct: 280 LGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLN 339

Query: 353 HTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWV 412
           HTAEGNE GPIFSFRGVDN+VYYMLAP GEFYNYSGCGNT NCNHP VR+FI+DCLRYWV
Sbjct: 340 HTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWV 399

Query: 413 TEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQ 472
           TEMHVDGFRFDL SIM+R SSLWD  NVYG+ +EGD LTTG+P+  PP+IDMISNDPIL+
Sbjct: 400 TEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILR 459

Query: 473 GVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPN 532
           GVKLIAEAWDAGGLYQVG FPHWGIWSEWNGKFRD VRQFIKGTDGFSGAFAECLCGSPN
Sbjct: 460 GVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPN 519

Query: 533 LYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEF 592
           LYQ GGRKPW SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F
Sbjct: 520 LYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDF 579

Query: 593 VSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTM 652
            SISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGH K GNNNTYCHDNY+NYFRWD  
Sbjct: 580 ASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKK 639

Query: 653 EESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVD 712
           EE+ SDF RFCR++  FR ECESLGLNDFPTA+RLQWHG  P  P+WSE SRFVAF+LVD
Sbjct: 640 EEAHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVD 699

Query: 713 SVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQYAH 772
           SVK EIY+AFN SHL   V+LP RPG RWEP VDT K SP+D +  DLPER+ A++QY H
Sbjct: 700 SVKKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRH 759

Query: 773 FLDTNLYPMLSYSSIILLLSPEK 796
           FLD N+YPMLSYSSIILLLSP K
Sbjct: 760 FLDANVYPMLSYSSIILLLSPIK 781

[Close]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 532/713 (74.61%), Postives = 600/713 (84.15%), Query Frame = 0 

Query: 82  VLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGD 141
           VL G+P P GA   DGGVNFA+Y+  A++A+LCL    DL+ + V +E+ LDPL NRTG+
Sbjct: 96  VLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGN 155

Query: 142 VWHVFLKGDFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVISRGEFGALGPDDNC 201
           VWHVF++G+ + MLYGY+FDG F+P  G YFD S +++DPYAKAVISRGE+G  GP  +C
Sbjct: 156 VWHVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDC 215

Query: 202 WPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTRHESSQSEFPGTYRGLVDK 261
           WPQMAGM+P     FDW+GDLP+++PQK+L+IYEMH+RGFT+H SS  E PGTY G + K
Sbjct: 216 WPQMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVEHPGTYIGAISK 275

Query: 262 LDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQ 321
           LDYLKELG+NC+ELMPCHEFNELEY+S +S     KMNFWGYST+ FFSPMIRYSS GI+
Sbjct: 276 LDYLKELGVNCVELMPCHEFNELEYFSCSS-----KMNFWGYSTINFFSPMIRYSSGGIR 335

Query: 322 NCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMG 381
           NCG+DAINEFK  V+EAHKRGIEVIMDVVFNHTAEGNE GPI SFRG+DN+ YYMLAP G
Sbjct: 336 NCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKG 395

Query: 382 EFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDPVNVY 441
           EFYNYSGCGNT NCNHP VR FIVDCLRYWVTEMHVDGFRFDLASIMTRG SLWDPVNVY
Sbjct: 396 EFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVY 455

Query: 442 GSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLYQVGTFPHWGIWSEW 501
           GSP+EGD  TTG+PL  PPLIDMISNDPIL  VKLIAEAWDAGGLYQVG FPHW IWSEW
Sbjct: 456 GSPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEW 515

Query: 502 NGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCSINFICAHDGFTLADLVT 561
           NGK+RD VRQFIKGTDGF+G FAECLCGSP+LYQ GGRKPW SINF+CAHDGFTLADLVT
Sbjct: 516 NGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVT 575

Query: 562 YNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI 621
           YN K+N +NGEDN DGENHN SWNCG+EGEF  +SVK+LRKRQMRNFFV LMVSQGVPM 
Sbjct: 576 YNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMF 635

Query: 622 CMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDFHRFCRLMTTFRSECESLGLNDF 681
            MGDEYGH K GNNNTYCHD+Y+NYFRWD  EE SSD  RFC LMT FR +CESLGL DF
Sbjct: 636 YMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEE-SSDLQRFCSLMTKFRKQCESLGLADF 695

Query: 682 PTAERLQWHGHTPRNPDWSEASRFVAFTLVDSVKGEIYIAFNASHLPVTVALPERPGCRW 741
           PTA+RL WHGH P  PDWSE SRFVAF+  D  KGEIY+AFNASHLP  V LPERPG RW
Sbjct: 696 PTAQRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERPGYRW 755

Query: 742 EPLVDTGKVSPFDFLASDLPERDLAIQQYAHFLDTNLYPMLSYSSIILLLSPE 795
           EPLVDTGK +P+DFL  DLP+R  A+  ++HFL++NLYPMLSYSSIIL L P+
Sbjct: 756 EPLVDTGKPAPYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILELQPD 802

[Close]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 2.3e-172
Identity = 349/787 (44.35%), Postives = 456/787 (57.94%), Query Frame = 0 

Query: 13  SASFLRSLDLSSLKRNAVRIRRRSGICFDLDVNSRMSASAIGVSSRGAETRTSVAVAEVA 72
           S+SF   + L  L R   R R      F   +  R   S + V+ R A  R  V   E +
Sbjct: 27  SSSFTIPMGL-KLSRRVTRAR-----IFSRKIKDR---STLKVTCRRAHER--VVEEEAS 86

Query: 73  EASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCLI---HLFDLQENRVAQE 132
             ++   F+V  G  +P G +  D G+NFA+++ NA S TLCL       D  ++    E
Sbjct: 87  TMTETKLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIE 146

Query: 133 ITLDPLTNRTGDVWHVFLKG-DFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVIS 192
           + LDP  N+TGD WH+ ++    N +LYGY+ DG    ++G  FD S +LLDPYAK V  
Sbjct: 147 LVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKG 206

Query: 193 RGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQH-PQKELIIYEMHVRGFTRHESS 252
              F   G     + Q  G     +  FDW  D    + P+K+L+IYEM+VR FT  ESS
Sbjct: 207 HSSF---GDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESS 266

Query: 253 QSE--FPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYST 312
             +    G+Y G ++K+ +L++LGIN +EL+P  EF+ELE     S   D+ +N WGYST
Sbjct: 267 GMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYST 326

Query: 313 VGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGP-IF 372
           V FF+PM RY+S   +     A  EFK +VK  H  GIEVI+DVV+NHT E ++  P   
Sbjct: 327 VNFFAPMSRYASG--EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTT 386

Query: 373 SFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDL 432
           SFRG+DN VYYML P  +  N+SGCGNTLNCNHP V   I+D LR+WVTE HVDGFRFDL
Sbjct: 387 SFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDL 446

Query: 433 ASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAG 492
           AS++ R                    T GSPL  PPLI  I+ D +L   K+IAE WD G
Sbjct: 447 ASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCG 506

Query: 493 GLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCS 552
           GLY VG FP+W  W+EWNG +RD+VR+FIKG  G  G+FA  + GS +LYQ   RKP+  
Sbjct: 507 GLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHG 566

Query: 553 INFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQ 612
           +NF+ AHDGFTL DLV+YN KHN ANGE  NDG N N+SWNCG EGE     +K LR RQ
Sbjct: 567 VNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQ 626

Query: 613 MRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDFHRFCR 672
           M+NF + LM+SQG PM+ MGDEYGH + GNNN+Y HD  +N F+W  ++    +  RF  
Sbjct: 627 MKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFS 686

Query: 673 LMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVDSVKG-EIYIAFN 732
            +  FR     L   +F T   + WH     N D SE S+F+AFTL D + G +IY+AFN
Sbjct: 687 EVIKFRHSHHVLKHENFLTQGEITWH---EDNWDNSE-SKFLAFTLHDGIGGRDIYVAFN 746

Query: 733 ASHLPVTVALPE-RPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQYAHFLDTNLYPML 790
           A    V   +P+  PG +W  + DT   SP DF+   +          A   DT  Y + 
Sbjct: 747 AHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGV----------AGVADT--YNVA 761

[Close]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 4.9e-167
Identity = 326/726 (44.90%), Postives = 428/726 (58.95%), Query Frame = 0 

Query: 80  FEVLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCL-IHLFDLQENRVAQ--EITLDPLT 139
           F+   G   P G +  +GG+NFA+++ +A+S  LCL +     ++ + A   E  LD   
Sbjct: 98  FKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLDQQK 157

Query: 140 NRTGDVWHVFLKG-DFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVISRGEFGAL 199
           N+TGD+WHV ++G   + +LYGY+  G     +G  FD S +LLDPYAK V  R  FG  
Sbjct: 158 NKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYFGVA 217

Query: 200 GPDDNCWPQMAGMVPTVNDEFDWEGDLPMQH-PQKELIIYEMHVRGFTRHESS--QSEFP 259
               +   Q  G     +  FDW  D  + + P+ +L+IYEM+VR FT  ESS   S   
Sbjct: 218 EEKSS---QHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSR 277

Query: 260 GTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVGFFSPM 319
           G+Y GL+DK+ +L ELG+N +EL+P  E++ELE+  Y +   D+ +N WGYST+ FF+PM
Sbjct: 278 GSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNP-RDHMVNTWGYSTINFFAPM 337

Query: 320 IRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDN 379
            RY+SAG       A  E K +VKE HK GIEVI+DVV+NHT E ++  P + SFRG+DN
Sbjct: 338 SRYASAG--GGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDN 397

Query: 380 NVYYM--LAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMT 439
            VYYM  L    E  N+SGCGNTLNCNHP V+  I+D LR+WV E H+DGFRFDLAS++ 
Sbjct: 398 KVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLC 457

Query: 440 RGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLYQV 499
           RG                     G PL  PPLI  I+ D +L   K+IAE WD GGLY V
Sbjct: 458 RGPD-------------------GCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYLV 517

Query: 500 GTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCSINFIC 559
           G FP+W  W+EWNGK+RD++R+FIKG  G  G FA  + GS +LYQ   RKP+  +NF+ 
Sbjct: 518 GRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVI 577

Query: 560 AHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFF 619
           AHDGFTL DLV+YN KHN ANGE   DG N N SWNCG EGE   ++V  LR RQM+NF 
Sbjct: 578 AHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFH 637

Query: 620 VCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDFHRFCRLMTTF 679
           V LM+SQG PM+ MGDEYGH + GNNN+Y HD  IN F+W+ +E+      RF   M  F
Sbjct: 638 VALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKF 697

Query: 680 RSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVD-SVKGEIYIAFNASHLP 739
           R     L  + F     + WH     N    + S+F+AFT+ D +  G+IY+AFNA    
Sbjct: 698 RHSNPILRRDRFLNKNDVTWHEDCWEN----QESKFLAFTVHDHNSGGDIYLAFNAHDYF 757

Query: 740 VTVALPERPGCR-WEPLVDTGKVSPFDFLASDLPERDLAIQQYAHFLDTNLYPMLSYSSI 794
           V   +P  P  + W  +VDT   SP D +   +P                 Y +  YSSI
Sbjct: 758 VDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVP------------FTGPKYRIAPYSSI 782

[Close]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 7.3e-131
Identity = 266/611 (43.54%), Postives = 347/611 (56.79%), Query Frame = 0 

Query: 82  VLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGD 141
           V  G   P GA     G NF++++  A    LCLI      E+ V   I LD +    G 
Sbjct: 19  VWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLI-----DEDGVESRIPLDEV---DGY 78

Query: 142 VWHVFLKGDFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVISRGEFG-------- 201
           VWH +L        YG++  G F P  G   DPS++LLDPY K+      FG        
Sbjct: 79  VWHAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDV 138

Query: 202 -ALGPD------DNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTR-HE 261
            A+ PD      D+    M  +V  +N  FDW  D   + P  E +IYE HV+G T+ H 
Sbjct: 139 NAVDPDSTPPMVDSLGHTMTSVV--INPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHP 198

Query: 262 SSQSEFPGTYRGLVDK--LDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKM-NFWG 321
           S   E  GTY GL     +D+L EL +  +ELMP H+F       ++S L D  + N+WG
Sbjct: 199 SIPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF------LHDSRLLDLGLRNYWG 258

Query: 322 YSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGP 381
           Y+T GFF+P  +Y+S   +  G  A+ EFK +V+  H+ GIEVI+DVV+NHTAEGN  GP
Sbjct: 259 YNTFGFFAPHHQYAST--RQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGP 318

Query: 382 IFSFRGVDNNVYYMLAPMG-EFY-NYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGF 441
             +FRG+DN  YY L      FY +++G GN+LN  HP   + I+D LRYWV EMHVDGF
Sbjct: 319 TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGF 378

Query: 442 RFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEA 501
           RFDLAS + R     D ++ +                     D++  DP++  VKLIAE 
Sbjct: 379 RFDLASTLARELHDVDRLSAF--------------------FDLVQQDPVVSQVKLIAEP 438

Query: 502 WDAG-GLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGR 561
           WD G G YQVG FP  G+W+EWNGK+RD VR + +G     G FA  L GS +LY+  GR
Sbjct: 439 WDVGEGGYQVGNFP--GLWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGR 498

Query: 562 KPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKK 621
           +P  SINF+ AHDGFTL DLV+YN KHN ANGE+N DGE++N SWNCG EG      +  
Sbjct: 499 RPSASINFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILA 558

Query: 622 LRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDF 671
           LR RQMRN +  LMVSQG PMI  GDE G  + GNNN YC D+ +++  W ++ + ++D 
Sbjct: 559 LRARQMRNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDW-SLVDKNADL 587

[Close]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 712/808 (88.12%), Postives = 741/808 (91.71%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S K N + IRRRS I  DL V   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV VAE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPL NRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS FPGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFKLL+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPERPG +WEPLVDTGK+SPFDFL SDL ERD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLLSPEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLSPEKHAS 808

[Close]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 1488.4 bits (3852), Expect = 0.0e+00
Identity = 711/808 (88.00%), Postives = 740/808 (91.58%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDV---NSRMSA 60
           ME+ TRSAS  SS SFLR+ + S       S KRN + IRRRS I  DLDV   +SR++ 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    SSRGAE RTSV +AE  E S+ NRFEV EG PTPFGA  RDGG+NFAIY+GNA S
Sbjct: 61  SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCLI+LFDLQ+ RVAQEITLDPLTNRTGDVWHVFLKGDF +MLYGYKF GKFSP+EGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISR EFGALGPDDNCWPQMAGMVPTV+DEFDWEGDLP+QHPQK+
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDEFDWEGDLPLQHPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESSQS  PGTY GLVDKLDYLKELG+NCIELMPCHEFNELEYYSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYYSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYK+NFWGYSTVGF SPMIRYSSAGI+NCGQDAINEFKLL+KEAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WV EMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGDTLTTGSPLGNPPLIDMISNDPI
Sbjct: 421 WVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPI 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHW +WSEWNGK+RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYF WD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            MEESSSDFHRFCRLMTTFR ECESLGLNDFPTA+RLQWHGH+P NPDWSEASRFVAFTL
Sbjct: 661 KMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVK EIYIAFNASHLPVTV LPER G RWEPLVDTGK+SPFDFL SDL ERD+AIQQY
Sbjct: 721 VDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPMLSYSSIILLL PEKHAS
Sbjct: 781 AHFLDANLYPMLSYSSIILLLFPEKHAS 808

[Close]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 687/808 (85.02%), Postives = 734/808 (90.84%), Query Frame = 0 

Query: 1   MEIATRSASRYSSASFL-------RSLDLSSLKRNAVRIRRRSGICFDLD---VNSRMSA 60
           ME+ TRS S   SA FL             S K NAVR RRRS I  DLD   +NSRM  
Sbjct: 1   MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60

Query: 61  SAIGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAAS 120
           S    S+RGAE  TSVA+AE  E S+L+RFEV EG P P GA  RDGG+NFA+Y+GNA S
Sbjct: 61  SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120

Query: 121 ATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGC 180
           ATLCL++LFDLQENRV +E+TLDPLTN+TGDVWHVFLKG F EMLYGYKFDG+FSP++GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKE 240
           YFDPSRILLDPYAKAVISRGEFGA GPD+NCWPQMAGMVPTV DEFDWEGDLP+Q+PQK+
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDEFDWEGDLPLQYPQKD 240

Query: 241 LIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYN 300
           LIIYEMHVRGFTRHESS SEFPGTY GLV+KLDYLKELG+NCIELMPCHEFNELEY+SYN
Sbjct: 241 LIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVV 360
           SVLGDYKMNFWGYSTVGFFSPMIRYSSAGI+ CGQDAINEFKLL++EAHKRGIEVIMDVV
Sbjct: 301 SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 FNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRY 420
           FNHTAEGNENGPIFSFRGVDN+VYYMLAPMGEFYNYSGCGNT NCNHPAVRRFIVDCLRY
Sbjct: 361 FNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPI 480
           WVTEMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGD LTTGSPLGNPPLIDMISNDP+
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWG+WSEWNG++RD VRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQ GGRKPW SINFICAHDGFTLADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWD 660
           EFVS+SVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNYINYFRWD
Sbjct: 601 EFVSMSVKKLRKRQMRNFFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWD 660

Query: 661 TMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTL 720
            +EESSSDFHRFC L+TTFR ECESLGLNDFPTAERLQWHG++P  PDWSEASRFVAFTL
Sbjct: 661 KLEESSSDFHRFCSLVTTFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTL 720

Query: 721 VDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQY 780
           VDSVKGEIYIAFNA+HLPVTV LPERPG RWEPLVDTGK+SPFDFL+SDLPERD+AI+QY
Sbjct: 721 VDSVKGEIYIAFNANHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQY 780

Query: 781 AHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           AHFLD NLYPML YSSIILLLSP + +S
Sbjct: 781 AHFLDNNLYPMLCYSSIILLLSPAEASS 808

[Close]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 690/809 (85.29%), Postives = 732/809 (90.48%), Query Frame = 0 

Query: 1   MEIATRS-ASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDVNSRMSASA 60
           ME+ TRS AS +SSASFL   +LS       +LK+N VR RRR+ ICFDLD N  + +S 
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDAN-WIHSSL 60

Query: 61  IGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASAT 120
               +RGAE  TSVAVAE  E S+LNRFEV +G PTPFGA  RD G+NFAIY+ NA SAT
Sbjct: 61  NRARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAVSAT 120

Query: 121 LCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGCYF 180
           LCL+HL DLQEN V QEITLDPLTN+TGDVWHVFLKGDF EMLYGYKFDGKFSP+EGCYF
Sbjct: 121 LCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYF 180

Query: 181 DPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDE---FDWEGDLPMQHPQK 240
           DPSRILLDPYAKAVISRGEFGALGP+DNCWPQMAGMVPT N++   FDWEGDLP+QH QK
Sbjct: 181 DPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQHAQK 240

Query: 241 ELIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSY 300
           +LIIYEMHVRGFTRHESSQS FPGTY GLV+KLDYLKELG+NCIELMPCHEFNELEY+SY
Sbjct: 241 DLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSY 300

Query: 301 NSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDV 360
           NSVLGDYKMNFWGYSTV +FSPMIRYSSAGI NCGQDAINEFKLL++EAHKRGIEVIMDV
Sbjct: 301 NSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDV 360

Query: 361 VFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLR 420
           VFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVR FIVDCLR
Sbjct: 361 VFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLR 420

Query: 421 YWVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDP 480
           YWVTEMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGD LTTGSPLGNPPLIDMISNDP
Sbjct: 421 YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDP 480

Query: 481 ILQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCG 540
           +LQGVKLIAEAWDAGGLYQVGTFPHWG+WSEWNGK+RD VRQFIKGTDGFSGAFAECLCG
Sbjct: 481 VLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCG 540

Query: 541 SPNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE 600
           SPNLYQ GGRKP  SINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE
Sbjct: 541 SPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE 600

Query: 601 GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRW 660
           GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNY+NYFRW
Sbjct: 601 GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRW 660

Query: 661 DTMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFT 720
           D MEES+SDFHRFC LMTTFR ECESLGLNDFPT ERLQWHGH+P  PDW+EASRFVAFT
Sbjct: 661 DKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFT 720

Query: 721 LVDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQ 780
           LVDSVK E+YIAFNASHLPVTV LPERPG RW+PLVDTGK SPFDFL +D+PER+LAIQQ
Sbjct: 721 LVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQ 780

Query: 781 YAHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           YAHFLD NLYPMLSYSSIILLLSPE+  S
Sbjct: 781 YAHFLDANLYPMLSYSSIILLLSPEEPVS 808

[Close]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 690/809 (85.29%), Postives = 732/809 (90.48%), Query Frame = 0 

Query: 1   MEIATRS-ASRYSSASFLRSLDLS-------SLKRNAVRIRRRSGICFDLDVNSRMSASA 60
           ME+ TRS AS +SSASFL   +LS       +LK+N VR RRR+ ICFDLD N  + +S 
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDAN-WIHSSL 60

Query: 61  IGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASAT 120
               +RGAE  TSVAVAE  E S+LNRFEV +G PTPFGA  RD G+NFAIY+ NA SAT
Sbjct: 61  NRARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAVSAT 120

Query: 121 LCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGCYF 180
           LCL+HL DLQEN V QEITLDPLTN+TGDVWHVFLKGDF EMLYGYKFDGKFSP+EGCYF
Sbjct: 121 LCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYF 180

Query: 181 DPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDE---FDWEGDLPMQHPQK 240
           DPSRILLDPYAKAVISRGEFGALGP+DNCWPQMAGMVPT N++   FDWEGDLP+QH QK
Sbjct: 181 DPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQHAQK 240

Query: 241 ELIIYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSY 300
           +LIIYEMHVRGFTRHESSQS FPGTY GLV+KLDYLKELG+NCIELMPCHEFNELEY+SY
Sbjct: 241 DLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSY 300

Query: 301 NSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDV 360
           NSVLGDYKMNFWGYSTV +FSPMIRYSSAGI NCGQDAINEFKLL++EAHKRGIEVIMDV
Sbjct: 301 NSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDV 360

Query: 361 VFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLR 420
           VFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNT NCNHPAVR FIVDCLR
Sbjct: 361 VFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLR 420

Query: 421 YWVTEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDP 480
           YWVTEMHVDGFRFDLASIMTRGSSLWD VNVYG+PIEGD LTTGSPLGNPPLIDMISNDP
Sbjct: 421 YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDP 480

Query: 481 ILQGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCG 540
           +LQGVKLIAEAWDAGGLYQVGTFPHWG+WSEWNGK+RD VRQFIKGTDGFSGAFAECLCG
Sbjct: 481 VLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCG 540

Query: 541 SPNLYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE 600
           SPNLYQ GGRKP  SINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE
Sbjct: 541 SPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQE 600

Query: 601 GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRW 660
           GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGH K GNNNTYCHDNY+NYFRW
Sbjct: 601 GEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRW 660

Query: 661 DTMEESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFT 720
           D MEES+SDFHRFC LMTTFR ECESLGLNDFPT ERLQWHGH+P  PDW+EASRFVAFT
Sbjct: 661 DKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFT 720

Query: 721 LVDSVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQ 780
           LVDSVK E+YIAFNASHLPVTV LPERPG RW+PLVDTGK SPFDFL +D+PER+LAIQQ
Sbjct: 721 LVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQ 780

Query: 781 YAHFLDTNLYPMLSYSSIILLLSPEKHAS 799
           YAHFLD NLYPMLSYSSIILLLSPE+  S
Sbjct: 781 YAHFLDANLYPMLSYSSIILLLSPEEPVS 808

[Close]
BLAST of Sed0017236 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 566/743 (76.18%), Postives = 642/743 (86.41%), Query Frame = 0 

Query: 53  IGVSSRGAETRTSVAVAEVAEASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASAT 112
           I +S++   +  +  +A V +    +RF + +G+P+PFG  VRD GVNF++Y+ N+ SAT
Sbjct: 40  ISISAKDRRSNEAENIAVVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSAT 99

Query: 113 LCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLKGDFNEMLYGYKFDGKFSPREGCYF 172
           +CLI L DL++N+V +EI LDP  NRTG VWHVFL+GDF +MLYGY+FDGKFSP EG Y+
Sbjct: 100 ICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYY 159

Query: 173 DPSRILLDPYAKAVISRGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELI 232
           D S ILLDPYAKA+ISR EFG LGPDDNCWPQMA MVPT  +EFDWEGD+ ++ PQK+L+
Sbjct: 160 DSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLV 219

Query: 233 IYEMHVRGFTRHESSQSEFPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSV 292
           IYEMHVRGFTRHESS+ EFPGTY+G+ +KLD+LKELGINCIELMPCHEFNELEYYSYN++
Sbjct: 220 IYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTI 279

Query: 293 LGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFN 352
           LGD+++NFWGYST+GFFSPMIRY+SA   N    AINEFK+LVKEAHKRGIEVIMDVV N
Sbjct: 280 LGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLN 339

Query: 353 HTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWV 412
           HTAEGNE GPIFSFRGVDN+VYYMLAP GEFYNYSGCGNT NCNHP VR+FI+DCLRYWV
Sbjct: 340 HTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWV 399

Query: 413 TEMHVDGFRFDLASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQ 472
           TEMHVDGFRFDL SIM+R SSLWD  NVYG+ +EGD LTTG+P+  PP+IDMISNDPIL+
Sbjct: 400 TEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILR 459

Query: 473 GVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPN 532
           GVKLIAEAWDAGGLYQVG FPHWGIWSEWNGKFRD VRQFIKGTDGFSGAFAECLCGSPN
Sbjct: 460 GVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPN 519

Query: 533 LYQEGGRKPWCSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEF 592
           LYQ GGRKPW SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F
Sbjct: 520 LYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDF 579

Query: 593 VSISVKKLRKRQMRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTM 652
            SISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGH K GNNNTYCHDNY+NYFRWD  
Sbjct: 580 ASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKK 639

Query: 653 EESSSDFHRFCRLMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVD 712
           EE+ SDF RFCR++  FR ECESLGLNDFPTA+RLQWHG  P  P+WSE SRFVAF+LVD
Sbjct: 640 EEAHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVD 699

Query: 713 SVKGEIYIAFNASHLPVTVALPERPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQYAH 772
           SVK EIY+AFN SHL   V+LP RPG RWEP VDT K SP+D +  DLPER+ A++QY H
Sbjct: 700 SVKKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRH 759

Query: 773 FLDTNLYPMLSYSSIILLLSPEK 796
           FLD N+YPMLSYSSIILLLSP K
Sbjct: 760 FLDANVYPMLSYSSIILLLSPIK 781

[Close]
BLAST of Sed0017236 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 607.4 bits (1565), Expect = 1.6e-173
Identity = 349/787 (44.35%), Postives = 456/787 (57.94%), Query Frame = 0 

Query: 13  SASFLRSLDLSSLKRNAVRIRRRSGICFDLDVNSRMSASAIGVSSRGAETRTSVAVAEVA 72
           S+SF   + L  L R   R R      F   +  R   S + V+ R A  R  V   E +
Sbjct: 27  SSSFTIPMGL-KLSRRVTRAR-----IFSRKIKDR---STLKVTCRRAHER--VVEEEAS 86

Query: 73  EASDLNRFEVLEGVPTPFGAAVRDGGVNFAIYAGNAASATLCLI---HLFDLQENRVAQE 132
             ++   F+V  G  +P G +  D G+NFA+++ NA S TLCL       D  ++    E
Sbjct: 87  TMTETKLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIE 146

Query: 133 ITLDPLTNRTGDVWHVFLKG-DFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAVIS 192
           + LDP  N+TGD WH+ ++    N +LYGY+ DG    ++G  FD S +LLDPYAK V  
Sbjct: 147 LVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKG 206

Query: 193 RGEFGALGPDDNCWPQMAGMVPTVNDEFDWEGDLPMQH-PQKELIIYEMHVRGFTRHESS 252
              F   G     + Q  G     +  FDW  D    + P+K+L+IYEM+VR FT  ESS
Sbjct: 207 HSSF---GDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESS 266

Query: 253 QSE--FPGTYRGLVDKLDYLKELGINCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYST 312
             +    G+Y G ++K+ +L++LGIN +EL+P  EF+ELE     S   D+ +N WGYST
Sbjct: 267 GMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYST 326

Query: 313 VGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGP-IF 372
           V FF+PM RY+S   +     A  EFK +VK  H  GIEVI+DVV+NHT E ++  P   
Sbjct: 327 VNFFAPMSRYASG--EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTT 386

Query: 373 SFRGVDNNVYYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDL 432
           SFRG+DN VYYML P  +  N+SGCGNTLNCNHP V   I+D LR+WVTE HVDGFRFDL
Sbjct: 387 SFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDL 446

Query: 433 ASIMTRGSSLWDPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAG 492
           AS++ R                    T GSPL  PPLI  I+ D +L   K+IAE WD G
Sbjct: 447 ASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCG 506

Query: 493 GLYQVGTFPHWGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCS 552
           GLY VG FP+W  W+EWNG +RD+VR+FIKG  G  G+FA  + GS +LYQ   RKP+  
Sbjct: 507 GLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHG 566

Query: 553 INFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQ 612
           +NF+ AHDGFTL DLV+YN KHN ANGE  NDG N N+SWNCG EGE     +K LR RQ
Sbjct: 567 VNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQ 626

Query: 613 MRNFFVCLMVSQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEESSSDFHRFCR 672
           M+NF + LM+SQG PM+ MGDEYGH + GNNN+Y HD  +N F+W  ++    +  RF  
Sbjct: 627 MKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFS 686

Query: 673 LMTTFRSECESLGLNDFPTAERLQWHGHTPRNPDWSEASRFVAFTLVDSVKG-EIYIAFN 732
            +  FR     L   +F T   + WH     N D SE S+F+AFTL D + G +IY+AFN
Sbjct: 687 EVIKFRHSHHVLKHENFLTQGEITWH---EDNWDNSE-SKFLAFTLHDGIGGRDIYVAFN 746

Query: 733 ASHLPVTVALPE-RPGCRWEPLVDTGKVSPFDFLASDLPERDLAIQQYAHFLDTNLYPML 790
           A    V   +P+  PG +W  + DT   SP DF+   +          A   DT  Y + 
Sbjct: 747 AHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGV----------AGVADT--YNVA 761

[Close]
BLAST of Sed0017236 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 337.8 bits (865), Expect = 2.4e-92
Identity = 223/697 (31.99%), Postives = 343/697 (49.21%), Query Frame = 0 

Query: 85  GVPTPFG--AAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGDV 144
           G P P G  +   D   NF+ ++ ++ +  LCL    D   ++ A E+ LDP  NRTGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 145 WHVFLKGDFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAV---ISRGEFGALGPDD 204
           WH  +   ++ + YGY+       +E    +   I+LDPYA  V   +S+   G+L    
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSL---- 349

Query: 205 NCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTRHESSQ--SEFPGTYRG 264
                      + +  FDW  D+    P ++L++Y ++V+GFT+H SS+  S   GT+ G
Sbjct: 350 -----------SKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSG 409

Query: 265 LVDKLDYLKELGINCIELMPCHEFNELE--YYSYNSVLGDYKMNFWGYSTVGFFSPMIRY 324
           + +K+ +LK LG N + L P   F+E +  Y+ ++                 FFSPM  Y
Sbjct: 410 VAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY 469

Query: 325 SSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYY 384
              G  N  + A+N  K++VK+ H  GIEV+++VVF HTA+        + RG+D++ YY
Sbjct: 470 ---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYY 529

Query: 385 MLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLW 444
                 +  + S     LNCN+P V++ +++ LRYWVTE HVDGF F  AS + RG    
Sbjct: 530 YKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG---- 589

Query: 445 DPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLY-QVGTFPH 504
                            G  L  PPL++ I+ DP+L   KLIA+ WD   +  +   FPH
Sbjct: 590 ---------------VHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPH 649

Query: 505 WGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCSINFICAHDGF 564
           W  W+E N ++  NVR F++G  G     A  +CGS +++ + GR P  S N+I  + G 
Sbjct: 650 WKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVFTD-GRGPAFSFNYISRNSGL 709

Query: 565 TLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMV 624
           +L D+V++ S   LA+            SWNCG+EG     +V + R +Q+RNF     +
Sbjct: 710 SLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYI 769

Query: 625 SQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEES-SSDFHRFCRLMTTFRS-E 684
           S GVP++ MGDE G + RG+            F W+ +  +  +   +F   MT+ R+  
Sbjct: 770 SLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQITQFISFMTSVRARR 829

Query: 685 CESLGLNDFPTAERLQWHGHTPRNPDWSE-ASRFVAFTLVDSVKGE-------------- 744
            +     DF   E + W+ +    P W + AS+F+A  +    + E              
Sbjct: 830 SDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSN 843

Query: 745 -IYIAFNASHLPVTVALPERP-GCRWEPLVDTGKVSP 753
            ++I FNAS  P +V LP  P G +W  LVDT    P
Sbjct: 890 DLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFP 843

[Close]
BLAST of Sed0017236 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 337.8 bits (865), Expect = 2.4e-92
Identity = 223/697 (31.99%), Postives = 343/697 (49.21%), Query Frame = 0 

Query: 85  GVPTPFG--AAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGDV 144
           G P P G  +   D   NF+ ++ ++ +  LCL    D   ++ A E+ LDP  NRTGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 145 WHVFLKGDFNEMLYGYKFDGKFSPREGCYFDPSRILLDPYAKAV---ISRGEFGALGPDD 204
           WH  +   ++ + YGY+       +E    +   I+LDPYA  V   +S+   G+L    
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSL---- 349

Query: 205 NCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTRHESSQ--SEFPGTYRG 264
                      + +  FDW  D+    P ++L++Y ++V+GFT+H SS+  S   GT+ G
Sbjct: 350 -----------SKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSG 409

Query: 265 LVDKLDYLKELGINCIELMPCHEFNELE--YYSYNSVLGDYKMNFWGYSTVGFFSPMIRY 324
           + +K+ +LK LG N + L P   F+E +  Y+ ++                 FFSPM  Y
Sbjct: 410 VAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY 469

Query: 325 SSAGIQNCGQDAINEFKLLVKEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYY 384
              G  N  + A+N  K++VK+ H  GIEV+++VVF HTA+        + RG+D++ YY
Sbjct: 470 ---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYY 529

Query: 385 MLAPMGEFYNYSGCGNTLNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLW 444
                 +  + S     LNCN+P V++ +++ LRYWVTE HVDGF F  AS + RG    
Sbjct: 530 YKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG---- 589

Query: 445 DPVNVYGSPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGGLY-QVGTFPH 504
                            G  L  PPL++ I+ DP+L   KLIA+ WD   +  +   FPH
Sbjct: 590 ---------------VHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPH 649

Query: 505 WGIWSEWNGKFRDNVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWCSINFICAHDGF 564
           W  W+E N ++  NVR F++G  G     A  +CGS +++ + GR P  S N+I  + G 
Sbjct: 650 WKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVFTD-GRGPAFSFNYISRNSGL 709

Query: 565 TLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMV 624
           +L D+V++ S   LA+            SWNCG+EG     +V + R +Q+RNF     +
Sbjct: 710 SLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYI 769

Query: 625 SQGVPMICMGDEYGHAKRGNNNTYCHDNYINYFRWDTMEES-SSDFHRFCRLMTTFRS-E 684
           S GVP++ MGDE G + RG+            F W+ +  +  +   +F   MT+ R+  
Sbjct: 770 SLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQITQFISFMTSVRARR 829

Query: 685 CESLGLNDFPTAERLQWHGHTPRNPDWSE-ASRFVAFTLVDSVKGE-------------- 744
            +     DF   E + W+ +    P W + AS+F+A  +    + E              
Sbjct: 830 SDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSN 843

Query: 745 -IYIAFNASHLPVTVALPERP-GCRWEPLVDTGKVSP 753
            ++I FNAS  P +V LP  P G +W  LVDT    P
Sbjct: 890 DLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFP 843

[Close]
BLAST of Sed0017236 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 89.0 bits (219), Expect = 1.9e-17
Identity = 99/379 (26.12%), Postives = 147/379 (38.79%), Query Frame = 0 

Query: 89  PFGAAVRDGGVNFAIYAGNAASATLCLIHLFDLQENRVAQEITLDPLTNRTGDVWHVFLK 148
           P GA     GV+  ++A  A + ++C   ++    ++   EI   PL    G VW     
Sbjct: 207 PLGAHFTPEGVSLHLWAPTAQAVSVC---IYKNPLDKSPMEIC--PLKEANG-VWSTEGA 266

Query: 149 GDFNEMLYGYK---FDGKFSPREGCYFDPSRILLDPYAKAVISRGE---FGALGPDD--- 208
             +    Y YK   +       E CY +      DPYA+ + + G       L  DD   
Sbjct: 267 CSWGGCYYVYKVSVYHPSTMKLETCYAN------DPYARGLSADGRKTFLVNLDSDDLKP 326

Query: 209 NCWPQMAGMVPTVNDEFDWEGDLPMQHPQKELIIYEMHVRGFTRH-ESSQSEFPGTYRGL 268
             W  +A   P +    D             + IYE+HVR F+ + E+ + E  G Y   
Sbjct: 327 EGWDNLADKKPCLRSFSD-------------ISIYELHVRDFSANDETVEPENRGGYLAF 386

Query: 269 VDK-------LDYLKELGINCIELMPCHEFNELEYYSYN--------------------- 328
             K       L  L + G+  + L+P  +F +++    N                     
Sbjct: 387 TSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQA 446

Query: 329 ---SVLGDYKMNFWGYSTVGFFSPMIRYSSAGIQNCGQDAINEFKLLVKEAHKRGIEVIM 388
               +  D   N WGY+ V +  P   Y+S     C    I EF+ +V+  +  G+ V++
Sbjct: 447 RITEIQNDDGYN-WGYNPVLWGVPKGSYASDPTGPC---RIIEFRKMVQALNCTGLNVVL 506

Query: 389 DVVFNHTAEGNENGPIFSFRGVDNNV--YYMLAPMGEFYNYSGCGNTLNCNHPAVRRFIV 425
           DVV+NH    + +GP      +D  V  YY+      F   S C N     H  V R I 
Sbjct: 507 DVVYNHL---HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIR 553

The following BLAST results are available for this feature:
BLAST of Sed0017236 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022932124.10.0e+0088.12isoamylase 1, chloroplastic [Cucurbita moschata][more]
KAG7021674.10.0e+0088.00Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023531773.10.0e+0087.87isoamylase 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022973965.10.0e+0088.00isoamylase 1, chloroplastic [Cucurbita maxima][more]
KAG6587707.10.0e+0086.59Isoamylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Sed0017236 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O041960.0e+0076.18Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1[more]
D0TZF00.0e+0074.61Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... [more]
Q9M0S52.3e-17244.35Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2[more]
B9G4344.9e-16744.90Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... [more]
P0A4Y57.3e-13143.54Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935... [more]
BLAST of Sed0017236 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EW540.0e+0088.12isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 S... [more]
A0A6J1ICQ30.0e+0088.00isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=... [more]
A0A6J1BZK50.0e+0085.02isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 ... [more]
A0A5A7UQM50.0e+0085.29Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 ... [more]
A0A1S3BVW80.0e+0085.29isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1[more]
BLAST of Sed0017236 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G39930.10.0e+0076.18isoamylase 1 [more]
AT4G09020.11.6e-17344.35isoamylase 3 [more]
AT1G03310.22.4e-9231.99debranching enzyme 1 [more]
AT1G03310.12.4e-9231.99debranching enzyme 1 [more]
AT5G04360.11.9e-1726.12limit dextrinase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 235..670
e-value: 3.1E-9
score: 30.6
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 253..359
e-value: 7.9E-13
score: 48.7
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 69..211
e-value: 1.3E-36
score: 127.2
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 89..183
e-value: 5.8E-16
score: 58.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 212..684
e-value: 1.7E-207
score: 692.1
NoneNo IPR availablePANTHERPTHR43002GLYCOGEN DEBRANCHING ENZYMEcoord: 77..793
NoneNo IPR availablePANTHERPTHR43002:SF1ISOAMYLASE 1, CHLOROPLASTICcoord: 77..793
NoneNo IPR availableCDDcd11326AmyAc_Glg_debranchcoord: 216..672
e-value: 0.0
score: 754.304
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 686..789
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 686..799
e-value: 1.4E-32
score: 114.3
IPR044505Glycogen debranching enzyme GlgX/isoamylase, N-terminal Early set domainCDDcd02856E_set_GDE_Isoamylase_Ncoord: 89..235
e-value: 2.34038E-36
score: 131.23
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 213..676
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 85..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0017236.1Sed0017236.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005977 glycogen metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0019156 isoamylase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds