Sed0017048 (gene) Chayote v1

BLAST of Sed0017048 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 871.3 bits (2250), Expect = 4.5e-249
Identity = 421/568 (74.12%), Postives = 490/568 (86.27%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIK 62
           NAEGD+KKKK+AI+SVSSLILVAMVVAVTVGVN  P ++ G DG+G    EI++++KAI+
Sbjct: 7   NAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPKEEHG-DGHGGETHEISSTTKAIQ 66

Query: 63  AICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPMA 122
           AICQPTDYK+ CEK+LM+EAGNTTDPKELAKVGLR+AT  LN AIRNSTTLQELAKDP  
Sbjct: 67  AICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELAKDPRT 126

Query: 123 NQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFEN 182
           NQALQNCRELLEYAIDDLN+SF RIG+F+MSKLDEFV D+KIWLS ALTYEQTCLDGFEN
Sbjct: 127 NQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFEN 186

Query: 183 TTGDSGERMKEFLKSGKEMTSNGLVILGG-SGM--------KVGRRRLMVNYEEIPSWVS 242
           TTGD+G RM+EFLKS ++MT+NGL I+   SGM          GRR L  + +E+PSWVS
Sbjct: 187 TTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDADEMPSWVS 246

Query: 243 EGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVI 302
           EGKR+LMQA  AS+KPD+VVA+DGSGKYKTI AALA+VP KSN TFVIYVKAGIYKE V+
Sbjct: 247 EGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEIVV 306

Query: 303 VPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIK 362
           +PKA+ H+TM+GDGPTKT++TG+LN++DG+QTFKTATFAAIG NF  +DMGF N+AGA K
Sbjct: 307 IPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFENTAGAAK 366

Query: 363 HQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCE 422
           HQAVALRVQSDRS+F+NC +DGYQDTLY HAHRQF+RDC ISGTIDFVFGNAA +FQNC+
Sbjct: 367 HQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAATIFQNCK 426

Query: 423 LIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYS 482
           L+VRKP++NQ CIVTA GRL ++EPT L+FQ CHFMGD  YLPFK  NKAYLGRPWKQYS
Sbjct: 427 LVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLGRPWKQYS 486

Query: 483 RTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQV 542
           RTII+GSQIDD+IQP+GWLPW G F L+TLFY+E  NKGPGAD SKRV W GI+ ITPQ 
Sbjct: 487 RTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGIRHITPQH 546

Query: 543 AEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           A  +TPR+F+DGDAWI   G+PY+SGMM
Sbjct: 547 AADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of Sed0017048 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 862.8 bits (2228), Expect = 1.6e-246
Identity = 417/570 (73.16%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGK-DGNGEAKPEITASSKAI 62
           NAEGD+KKKKIAI+SVSSLILVAMVVAVTVG+N  P ++ G+ D   +  PEI++S+KAI
Sbjct: 7   NAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISSSTKAI 66

Query: 63  KAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPM 122
           +AICQPTDYKETCEKSLM+EAGNTTDPKEL KVG ++AT  LN AIRNSTTLQEL+KDP 
Sbjct: 67  QAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELSKDPR 126

Query: 123 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 182
            NQALQNCRELLEYAIDDLN+SF RIG+F+MSKLDEFV D+KIWLS ALTYEQTCLDGFE
Sbjct: 127 TNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFE 186

Query: 183 NTTGDSGERMKEFLKSGKEMTSNGL-------VILGG---SGMKVGRRRLMVNYEEIPSW 242
           NTTGD+G RM+EFLKS +EMT+NGL        ILG     GM  GRR L  +  E+PSW
Sbjct: 187 NTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGM-TGRRLLEDDDHEMPSW 246

Query: 243 VSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKET 302
           +S+GKR+LMQAG A +KPD++VA+DGSGKYKTI AALA+VP KSN TFVIYVKAGIYKE 
Sbjct: 247 ISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEI 306

Query: 303 VIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGA 362
           V++PK + H+TM+GDGPTKTV+TG+LN++DG+QTFKTATF+AIG NF  +DMGF N+AG+
Sbjct: 307 VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGS 366

Query: 363 IKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQN 422
            KHQAVALRVQSDRS+F+NC +DGYQDTLY HAHRQF+RDC ISGTIDFVFGNAA  FQN
Sbjct: 367 AKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQN 426

Query: 423 CELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQ 482
           C+L+VRKP++NQ CIVTA GR+ ++EPT L+FQGCHFMGD  YLPFK+ NKAYLGRPWKQ
Sbjct: 427 CKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQ 486

Query: 483 YSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITP 542
           YSRTII+GSQIDD+IQP+GWLPW G F L+TLFY+E  NKGPGA  +KRV W GIK ITP
Sbjct: 487 YSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGIKHITP 546

Query: 543 QVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           Q A  FTPR+F+DGDAWI   G+PY+SGMM
Sbjct: 547 QHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of Sed0017048 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 855.1 bits (2208), Expect = 3.3e-244
Identity = 420/571 (73.56%), Postives = 484/571 (84.76%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNP-AKDDGKDGNG-EAKPEITASSKA 62
           NAEGD+KKKKIAI+SVSSLILVAMVVAVTVGVN  P  ++ G D +G E  PEIT+S+KA
Sbjct: 7   NAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKA 66

Query: 63  IKAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDP 122
           I+AICQPTDYKETCEKSL +EAGNTTDPKEL KVG ++AT  LN AI+NSTTL+ELAKDP
Sbjct: 67  IQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDP 126

Query: 123 MANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGF 182
             NQALQNCRELLEYAIDDLN SF RIG+F+MSKLD+FV D+KIWLS ALTYEQTCLDGF
Sbjct: 127 RTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGF 186

Query: 183 ENTTGDSGERMKEFLKSGKEMTSNGL-------VILGG---SGMKVGRRRLMVNYEEIPS 242
           ENTTGD+G RM+EFLKS ++MT+NGL        ILG     GM  GRR L  +  E+PS
Sbjct: 187 ENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMS-GRRLLEDDDREMPS 246

Query: 243 WVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKE 302
           WVS+GKR+LMQAG A++KPD+VVA+DGSGKYKTI AALA+VP KSN TFVI+VKAGIYKE
Sbjct: 247 WVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKE 306

Query: 303 TVIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAG 362
            V++PK + H+TM+GDGPTKTV+TG+LN++DG+QTFKTATF+AIG NF  +DMGF N+AG
Sbjct: 307 IVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAG 366

Query: 363 AIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQ 422
           A KHQAVALRVQSDRS+F NC +DGYQDTLY HAHRQF+RDC ISGTIDFVFGNAA  FQ
Sbjct: 367 AAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQ 426

Query: 423 NCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWK 482
           NC+L+VRKPL+NQ CIVTA GRL ++EPT L+FQ CHFMGD  YLPFKA NKAYLGRPWK
Sbjct: 427 NCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWK 486

Query: 483 QYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKIT 542
           +YSRTII+GS IDD+IQP+GWLPW G F L+TLFY+E  NKG GAD SKRV W GIK IT
Sbjct: 487 EYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHIT 546

Query: 543 PQVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           PQ A  FTPR+F+DGDAWI   G+PY+SGMM
Sbjct: 547 PQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of Sed0017048 vs. NCBI nr
Match: XP_023515122.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.1 bits (2182), Expect = 3.4e-241
Identity = 411/568 (72.36%), Postives = 478/568 (84.15%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDG-KDGNGEAKPEITASSKAI 62
           +AEGD KKKK+A+V VSSL LVAMVVAVTVGVN +  K +G  DG+G+   EI++S+KAI
Sbjct: 6   DAEGDNKKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAI 65

Query: 63  KAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPM 122
           + IC+PTDYKETCE+SLMSE  NTTDP+EL K+G +V  +QLNSAIRNSTTLQ LAK+P 
Sbjct: 66  QTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR 125

Query: 123 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 182
            NQALQNCRELLEYAIDD+ DSF RIG+F+M+KLD+FV D+ +WLS ALTYEQTCLDGFE
Sbjct: 126 TNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFE 185

Query: 183 NTTGDSGERMKEFLKSGKEMTSNGLVI-------LGG-SGMKVGRRRLMVNYEEIPSWVS 242
           NTTGD+GE+MK+FLKS +EMTSNGL I       LG   G K GRR  ++  +E+PSWV+
Sbjct: 186 NTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKKGRR--LLAGDEVPSWVT 245

Query: 243 EGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVI 302
           + KR+LMQ  TA++ P+VVVA+DGSGKYKTI  AL EVPKKSN TFVIYVKAGIYKETV+
Sbjct: 246 DEKRRLMQLPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVL 305

Query: 303 VPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIK 362
           +PKAL HVTM+GDGPTKTV++G LN+VDG+QTFKTATFA +G NF  +DMGF N+AG+ K
Sbjct: 306 IPKALTHVTMYGDGPTKTVVSGGLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAK 365

Query: 363 HQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCE 422
           HQAVALRVQSDRS+F NC +DGYQDTLY   HRQF+RDCK+SGTIDFVFGN+AAVFQNC 
Sbjct: 366 HQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCS 425

Query: 423 LIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYS 482
           L+VRKPL NQ CIVTAQGRLE REPTGLVFQ CHFMGD  YLPFKA NKAYLGRPWKQ+S
Sbjct: 426 LVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFS 485

Query: 483 RTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQV 542
           RTIIMGS+IDDLIQP+GWLPW G F L+TLFY+EF NKGPGADL+ RV W GIK +TPQ 
Sbjct: 486 RTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWKGIKTLTPQQ 545

Query: 543 AEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           A  FTP+ F+DGD WI  TGVPY+ GMM
Sbjct: 546 AADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of Sed0017048 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.3 bits (2180), Expect = 5.9e-241
Identity = 413/576 (71.70%), Postives = 484/576 (84.03%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAK--DDGKDG-------NGEAKPE 62
           +AEGD+KKKKIA++SVSSLILVAMVVAVTVGVN  P +  DDG  G       +G+   +
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEK 65

Query: 63  ITASSKAIKAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTL 122
           +++S+KAIK ICQPTDYKETCE+SLM EAGNTTDPKEL K G ++A E+LN+AIRNSTTL
Sbjct: 66  VSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 QELAKDPMANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYE 182
           QELA+DP ANQALQNCRELLEYAIDDL+DS+ RIG+F +SK +EFVND+KIWLSAALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNY 242
           QTCLDGFENTTGDSGERMKEFLKS +EMT+NGL I+      +G         RRL+   
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAATGRRLLDT- 245

Query: 243 EEIPSWVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKA 302
            ++P WVS+GKR+LMQ GT ++KPD+VVA+DGSGKYKTI  AL +VP KSN TFVIYVKA
Sbjct: 246 -DMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKTFVIYVKA 305

Query: 303 GIYKETVIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGF 362
           GIYKE V++PK + H+TM+GDGPTK+V+TGNLN+VDG+QTFKTATFAAIG NF  +DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGF 365

Query: 363 VNSAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNA 422
           VN+AG  KHQAVALRVQSDR++ +NC +DGYQDTLY HA+RQF+RDC ISGTIDFVFGN+
Sbjct: 366 VNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNS 425

Query: 423 AAVFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYL 482
           AA+FQNC+L+VRKPL+NQ CIVTA GRL +REPT L+FQGCHFMGD  YLPFK  NKAYL
Sbjct: 426 AAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFKKVNKAYL 485

Query: 483 GRPWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPG 542
           GRPWKQYSRTIIMGSQIDDLIQP+GWLPW G FAL+TLFY+E  N+GPGAD S RV W G
Sbjct: 486 GRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRG 545

Query: 543 IKKITPQVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           I+ ITPQ A  +T R+F+DGDAWI   GVPY+SGMM
Sbjct: 546 IRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Sed0017048 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 628.2 bits (1619), Expect = 8.7e-179
Identity = 304/564 (53.90%), Postives = 403/564 (71.45%), Query Frame = 0

Query: 8   QKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAICQP 67
           ++K++  ++++SS++L++MVVAVTVGV+ N       DG+ + K E+ AS KA+K +C P
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNK-----HDGDSKGKAEVNASVKAVKDVCAP 69

Query: 68  TDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPMANQALQ 127
           TDY++TCE +L+    NTTDP EL K    V  +Q+  A + S T+ EL KD     AL 
Sbjct: 70  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 129

Query: 128 NCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTTGDS 187
            C+EL++YA+D+L++SF  +G FE   LDE + +++IWLSAA+++E+TCL+GF+ T G++
Sbjct: 130 QCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNA 189

Query: 188 GERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNYEEIPSWVSEGKRQL 247
           GE MK+ LK+  E+T NGL I+      VG+        RRL+   E  PSWV +  R+L
Sbjct: 190 GETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA--EGFPSWVDQRGRKL 249

Query: 248 MQAGTA--SLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVIVPKA 307
           +QA  A   +KPD+VVA+DGSG+YKTI  AL  VPKK NTTFV+++KAG+YKE V V K 
Sbjct: 250 LQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKT 309

Query: 308 LRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIKHQAV 367
           + H+   GDGP KT+I+GN NY DG+ T++TAT A +G  F+ K++GF N+AGAIKHQAV
Sbjct: 310 MSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAV 369

Query: 368 ALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCELIVR 427
           A+RVQSD S+F NC  DGYQDTLYTH+HRQFFRDC ISGTIDF+FG+AAAVFQNC L+VR
Sbjct: 370 AVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVR 429

Query: 428 KPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYSRTII 487
           KPL NQ C +TA GR + RE TG VFQGC   G+  YL  K  +KAYLGRPWK+YSRTII
Sbjct: 430 KPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTII 489

Query: 488 MGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQVAEGF 547
           M + I D +QPQGW PW G F L TLFYSE  N GPG+ L+ RVTW GIK ++ +    F
Sbjct: 490 MNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKF 549

Query: 548 TPRKFMDGDAWIKPTGVPYASGMM 562
           TP +++ GD WI   GVPY +G++
Sbjct: 550 TPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Sed0017048 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 4.4e-175
Identity = 296/564 (52.48%), Postives = 402/564 (71.28%), Query Frame = 0

Query: 5   EGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAI 64
           E  ++KK+  I+S+SS++L++MVVAVT+GV+ N + + G +       EIT S KAIK +
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDE-------EITTSVKAIKDV 67

Query: 65  CQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPMANQ 124
           C PTDYKETCE +L  +A +T+DP EL K       +Q++   + S T+ EL KDP A  
Sbjct: 68  CAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKM 127

Query: 125 ALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTT 184
           AL  C+EL++YAI +L+ SF  +G FE  K+DE +  ++IWLSA +++EQTCLDGF+ T 
Sbjct: 128 ALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQ 187

Query: 185 GDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNYEEIPSWVSEGK 244
           G++GE +K+ LK+  ++T NGL ++      +G+        RRL+   +E PSW+    
Sbjct: 188 GNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL--SQEFPSWMDARA 247

Query: 245 RQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVIVPK 304
           R+L+ A  + +KPD+VVA+DGSG+YKTI  AL  VPKK NTTFV+++K GIYKE V V +
Sbjct: 248 RRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNR 307

Query: 305 ALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIKHQA 364
           ++ H+   GDGP KTVI+G+ +Y DG+ T+KTAT A +G +F+ K++ F N+AGAIKHQA
Sbjct: 308 SMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQA 367

Query: 365 VALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCELIV 424
           VA+RV +D S+F+NC  DGYQDTLY H+HRQF+RDC ISGTIDF+FG+AAAVFQNC L+V
Sbjct: 368 VAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLV 427

Query: 425 RKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYSRTI 484
           RKPL NQ C +TA GR + RE TG V QGC  +G+  YL  K ++K YLGRPWK+YSRTI
Sbjct: 428 RKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTI 487

Query: 485 IMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQVAEG 544
           IM + I D + P+GW PW G F L+TLFYSE  N GPGA ++KRVTWPGIKK++ +    
Sbjct: 488 IMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILK 547

Query: 545 FTPRKFMDGDAWIKPTGVPYASGM 561
           FTP +++ GDAWI   GVPY  G+
Sbjct: 548 FTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Sed0017048 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.8e-155
Identity = 269/569 (47.28%), Postives = 375/569 (65.91%), Query Frame = 0

Query: 5   EGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAI 64
           +G+ KKKK  I  V + +LV MVVAV +  ++N +  +          +I  ++ A++A+
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSE-----KIVPVQIKTATTAVEAV 63

Query: 65  CQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTT--LQELAKDPMA 124
           C PTDYKETC  SLM  + ++T P +L K+G  V    +  +I+ ++     + A D   
Sbjct: 64  CAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDT 123

Query: 125 NQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFEN 184
             AL+ C +L+  A DDL         F + ++++FV D+++WLS ++ Y+QTC+D FE 
Sbjct: 124 KGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEE 183

Query: 185 TTGDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------------RRLMVNYEEI 244
           T     + M++  K+ +E+TSNGL ++      +G               R+L+   + I
Sbjct: 184 TNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGI 243

Query: 245 PSWVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIY 304
           PSWV    R+LM A    +K +VVVA DGSG+YKTI  AL  VPK +   FVIY+K G+Y
Sbjct: 244 PSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 303

Query: 305 KETVIVPKALRHVTMFGDGPTKTVITGNLNYVDG-VQTFKTATFAAIGPNFLGKDMGFVN 364
            E V V K + HVT  GDGPTKT ITG+LNY  G V+T+ TAT A  G NF  K++GF N
Sbjct: 304 NEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFEN 363

Query: 365 SAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAA 424
           +AG   HQAVALRV +D +VF+NC +DGYQDTLY H+HRQFFRDC +SGT+DF+FG+   
Sbjct: 364 TAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIV 423

Query: 425 VFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGR 484
           V QNC ++VRKP+++Q+C++TAQGR +KRE TGLV Q CH  G+  Y+P K+ NKAYLGR
Sbjct: 424 VLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGR 483

Query: 485 PWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIK 544
           PWK++SRTIIMG+ IDD+I P GWLPW G FAL+TL+Y+E+ N GPG++ ++RV WPGIK
Sbjct: 484 PWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIK 543

Query: 545 KITPQVAEGFTPRKFMDGDAWIKPTGVPY 557
           K++P+ A  FTP +F+ G+ WI P  VPY
Sbjct: 544 KLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Sed0017048 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 513.1 bits (1320), Expect = 4.1e-144
Identity = 262/576 (45.49%), Postives = 374/576 (64.93%), Query Frame = 0

Query: 4   AEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKA 63
           ++GD+KKK I   SVS   LV MVV+V V  +K+  KDD        +  I  ++KA++A
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSKHSPKDD--------ENHIRKTTKAVQA 62

Query: 64  ICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNST--TLQELAKDPM 123
           +C PTD+K+TC  SLM  + ++ DP +L K+G +V  + +N ++  ++     +  K+P 
Sbjct: 63  VCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPE 122

Query: 124 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 183
           A  A + C +L+  AIDDL         F + +++ FV D+++WLS ++ ++QTC+D F 
Sbjct: 123 AKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFG 182

Query: 184 NTTGDSGERMKEFLKSGKEMTSNGLVI---------------LGGSGMKVGRRRLMVNYE 243
               +  + M +  K+ +E++SN L +               L G+  K   R+L+   +
Sbjct: 183 EIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA-RKLLSTED 242

Query: 244 EIPSWVSEGKRQLMQA---GTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYV 303
            IP+WV    R+LM A   G   +K + VVA+DG+G++KTIT AL  VPK +   F+I++
Sbjct: 243 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHI 302

Query: 304 KAGIYKETVIVPKALRHVTMFGDGPTKTVITGNLNY-VDGVQTFKTATFAAIGPNFLGKD 363
           K GIYKE V V K + HVT  GDGP KT+ITG+LN+ +  V+TF TAT    G +F  K+
Sbjct: 303 KEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKN 362

Query: 364 MGFVNSAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVF 423
           +G  N+AG    QAVALRV +D +VFH+C +DG+QDTLY H+HRQF+RDC +SGT+DF+F
Sbjct: 363 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 422

Query: 424 GNAAAVFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENK 483
           G+A  + QNC+++VRKP + Q C+VTAQGR   RE TGLV  GCH  GD  Y+P K+ NK
Sbjct: 423 GDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNK 482

Query: 484 AYLGRPWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVT 543
           AYLGRPWK++SRTIIM + IDD+I P GWLPW+G FAL TL+Y+E  N GPG++ ++RV 
Sbjct: 483 AYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVK 542

Query: 544 WPGIKKITPQVAEGFTPRKFMDGDAWIKPTGVPYAS 559
           WPGIKK+TPQ A  +T  +F+ GD WI  T VPY +
Sbjct: 543 WPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Sed0017048 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.9e-126
Identity = 216/450 (48.00%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 126 LQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTTG 185
           ++ C E+L+YA+D ++ S   +  F+  KL E+  D+K+WL+  L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 186 DSGERMKEFLKSGKEMTSNGL--------VILGGSGMKVGRRRLMVNYEEIPSWVSEGKR 245
            +GE M + LK+  E++SN +        ++ G    +    R +++ + IPSWV++G R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 246 QLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVIVPKA 305
           +L+  G  +++P+ VVA+DGSG++KT+T AL  VP K+   FVI+VKAG+YKETV V K 
Sbjct: 121 RLLAGG--NVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 306 LRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIKHQAV 365
           + +VT+ GDGPTKT  TG+LNY DG+ T+ TATF   G NF+ KD+GF N+AG  KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 366 ALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCELIVR 425
           ALRV +D+++F+NC +DG+QDTLY  + RQF+RDC ISGTIDFVFG    VFQNC+L+ R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 426 KPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYSRTII 485
            P + Q C+VTA GR ++   + LVFQ  HF G+   L       +YLGRPWKQYS+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 486 MGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQVAEGF 545
           M S ID +  P+G++PW G+   +T  Y E+NNKGPGAD + RV W G+K +T  VA  +
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 546 TPRKFMD------GDAWIKPTGVPYASGMM 562
            P KF +       D WI  +GVPY+ G M
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of Sed0017048 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 7.7e-247
Identity = 417/570 (73.16%), Postives = 487/570 (85.44%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGK-DGNGEAKPEITASSKAI 62
           NAEGD+KKKKIAI+SVSSLILVAMVVAVTVG+N  P ++ G+ D   +  PEI++S+KAI
Sbjct: 7   NAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISSSTKAI 66

Query: 63  KAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPM 122
           +AICQPTDYKETCEKSLM+EAGNTTDPKEL KVG ++AT  LN AIRNSTTLQEL+KDP 
Sbjct: 67  QAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELSKDPR 126

Query: 123 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 182
            NQALQNCRELLEYAIDDLN+SF RIG+F+MSKLDEFV D+KIWLS ALTYEQTCLDGFE
Sbjct: 127 TNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFE 186

Query: 183 NTTGDSGERMKEFLKSGKEMTSNGL-------VILGG---SGMKVGRRRLMVNYEEIPSW 242
           NTTGD+G RM+EFLKS +EMT+NGL        ILG     GM  GRR L  +  E+PSW
Sbjct: 187 NTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGM-TGRRLLEDDDHEMPSW 246

Query: 243 VSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKET 302
           +S+GKR+LMQAG A +KPD++VA+DGSGKYKTI AALA+VP KSN TFVIYVKAGIYKE 
Sbjct: 247 ISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEI 306

Query: 303 VIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGA 362
           V++PK + H+TM+GDGPTKTV+TG+LN++DG+QTFKTATF+AIG NF  +DMGF N+AG+
Sbjct: 307 VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGS 366

Query: 363 IKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQN 422
            KHQAVALRVQSDRS+F+NC +DGYQDTLY HAHRQF+RDC ISGTIDFVFGNAA  FQN
Sbjct: 367 AKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQN 426

Query: 423 CELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQ 482
           C+L+VRKP++NQ CIVTA GR+ ++EPT L+FQGCHFMGD  YLPFK+ NKAYLGRPWKQ
Sbjct: 427 CKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQ 486

Query: 483 YSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITP 542
           YSRTII+GSQIDD+IQP+GWLPW G F L+TLFY+E  NKGPGA  +KRV W GIK ITP
Sbjct: 487 YSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGIKHITP 546

Query: 543 QVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           Q A  FTPR+F+DGDAWI   G+PY+SGMM
Sbjct: 547 QHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of Sed0017048 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.6e-244
Identity = 420/571 (73.56%), Postives = 484/571 (84.76%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNP-AKDDGKDGNG-EAKPEITASSKA 62
           NAEGD+KKKKIAI+SVSSLILVAMVVAVTVGVN  P  ++ G D +G E  PEIT+S+KA
Sbjct: 7   NAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKA 66

Query: 63  IKAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDP 122
           I+AICQPTDYKETCEKSL +EAGNTTDPKEL KVG ++AT  LN AI+NSTTL+ELAKDP
Sbjct: 67  IQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDP 126

Query: 123 MANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGF 182
             NQALQNCRELLEYAIDDLN SF RIG+F+MSKLD+FV D+KIWLS ALTYEQTCLDGF
Sbjct: 127 RTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGF 186

Query: 183 ENTTGDSGERMKEFLKSGKEMTSNGL-------VILGG---SGMKVGRRRLMVNYEEIPS 242
           ENTTGD+G RM+EFLKS ++MT+NGL        ILG     GM  GRR L  +  E+PS
Sbjct: 187 ENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMS-GRRLLEDDDREMPS 246

Query: 243 WVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKE 302
           WVS+GKR+LMQAG A++KPD+VVA+DGSGKYKTI AALA+VP KSN TFVI+VKAGIYKE
Sbjct: 247 WVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKE 306

Query: 303 TVIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAG 362
            V++PK + H+TM+GDGPTKTV+TG+LN++DG+QTFKTATF+AIG NF  +DMGF N+AG
Sbjct: 307 IVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAG 366

Query: 363 AIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQ 422
           A KHQAVALRVQSDRS+F NC +DGYQDTLY HAHRQF+RDC ISGTIDFVFGNAA  FQ
Sbjct: 367 AAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQ 426

Query: 423 NCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWK 482
           NC+L+VRKPL+NQ CIVTA GRL ++EPT L+FQ CHFMGD  YLPFKA NKAYLGRPWK
Sbjct: 427 NCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWK 486

Query: 483 QYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKIT 542
           +YSRTII+GS IDD+IQP+GWLPW G F L+TLFY+E  NKG GAD SKRV W GIK IT
Sbjct: 487 EYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHIT 546

Query: 543 PQVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           PQ A  FTPR+F+DGDAWI   G+PY+SGMM
Sbjct: 547 PQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of Sed0017048 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.7e-241
Identity = 414/576 (71.88%), Postives = 482/576 (83.68%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAK--DDGKDG-------NGEAKPE 62
           +AE D+KKKKIA++SVSSLILVAMVVAVTVGVN  P +  DDG  G       +G+   +
Sbjct: 6   DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDAEK 65

Query: 63  ITASSKAIKAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTL 122
           I++S+KAIK ICQPTDYKETCE+SLM EAGNTTDPKEL K G ++A E+LN+AIRNSTTL
Sbjct: 66  ISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 QELAKDPMANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYE 182
           QELA+DP ANQALQNCRELLEYAIDDL+DS+ RIG+F +SK +EFVND+KIWLSAALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNY 242
           QTCLDGFENTTGDSGERMKEFLKS +EMT+NGL I+      +G         RRL+   
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGRRLLDT- 245

Query: 243 EEIPSWVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKA 302
            +IP WVS+GKR+LMQ GT ++KPD+VVA+DGSGKYKTI  AL  VP KSN TFVIYVKA
Sbjct: 246 -DIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIYVKA 305

Query: 303 GIYKETVIVPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGF 362
           GIYKE V++PK + H+TM+GDGPTK+V+TGNLN+VDG+QTFKTATFAAIG NF  +DMGF
Sbjct: 306 GIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGF 365

Query: 363 VNSAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNA 422
           VN+AG  KHQAVALRVQSDR++ +NC +DGYQDTLY HA+RQF+RDC ISGTIDFVFGN+
Sbjct: 366 VNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNS 425

Query: 423 AAVFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYL 482
           AA+FQNC+L+VRKPL+NQ CIVTA GRL +REPT L+FQGCHFMGD  YLPFK  NKAYL
Sbjct: 426 AAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNKAYL 485

Query: 483 GRPWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPG 542
           GRPWKQYSRTIIMGSQIDDLIQP+GWLPW G FAL+TLFY+E  N+GPGAD S RV W G
Sbjct: 486 GRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRG 545

Query: 543 IKKITPQVAEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           I+ ITPQ A  +T R+F+DGDAWI   GVPY+SGMM
Sbjct: 546 IRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Sed0017048 vs. ExPASy TrEMBL
Match: A0A6J1ER80 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.8e-240
Identity = 409/568 (72.01%), Postives = 480/568 (84.51%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDG-KDGNGEAKPEITASSKAI 62
           +AEGD +KKK+A+V VSSL LVAMVVAVTVGVN +  K +G  +G+G+   EI++S+KAI
Sbjct: 6   DAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAI 65

Query: 63  KAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPM 122
           + IC+PTDYKETCE+SLMSE  NTTDP+EL K+G +V  +QLNSAIRNSTTLQ LAK+P 
Sbjct: 66  QTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR 125

Query: 123 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 182
           ANQALQNCRELLEYAIDD+ DSF RIG+F+M+KLD+FV D+ +WLS ALTYEQTCLDGFE
Sbjct: 126 ANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFE 185

Query: 183 NTTGDSGERMKEFLKSGKEMTSNGLVI-------LGG-SGMKVGRRRLMVNYEEIPSWVS 242
           NTTGD+GE+MK+FLKS +EMTSNGL I       LG   G K GRR  ++  +E+PSWVS
Sbjct: 186 NTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRR--LLAGDEVPSWVS 245

Query: 243 EGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVI 302
           + KR+LMQ  TA++ P+VVVA+DGSGKYKTI  AL EV KKSN TFVIYVKAGIYKETV+
Sbjct: 246 DEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVL 305

Query: 303 VPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIK 362
           +PKA+ HVTM+GDGPTKTV++G+LN+VDG+QTFKTATFA +G NF  +DMGF N+AGA K
Sbjct: 306 IPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATK 365

Query: 363 HQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCE 422
           HQAVALRVQSDRS+F NC++DG+QDTLY   HRQF+RDCK+SGTIDFVFGN+AAVFQNC 
Sbjct: 366 HQAVALRVQSDRSIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCN 425

Query: 423 LIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYS 482
           L+VRKPL NQ CIVTAQGRLE REPTGLVFQ CHFMGD  YLPFKA NKAYLGRPWKQ+S
Sbjct: 426 LVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFS 485

Query: 483 RTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQV 542
           RTIIMGS+IDDLIQP+GWLPW G F L+TLFY+EF NKGPGADL+ RV W GIK +TPQ 
Sbjct: 486 RTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQ 545

Query: 543 AEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           A  FTP+ F+DGD WI  TGVPY+ GMM
Sbjct: 546 AADFTPKIFIDGDDWILNTGVPYSPGMM 571

BLAST of Sed0017048 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.9e-239
Identity = 409/568 (72.01%), Postives = 472/568 (83.10%), Query Frame = 0

Query: 3   NAEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDG-KDGNGEAKPEITASSKAI 62
           +AEGD KKKK+A V VSSL LVAMVVAVTVGVN +  K +G  DG+G+   EI++S+KAI
Sbjct: 6   DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAI 65

Query: 63  KAICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPM 122
           + IC+PTDYKETCEKSLMSE  NTTDP+EL K+G +V  +QLNSAIRNSTTLQ LAK+P 
Sbjct: 66  QTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR 125

Query: 123 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 182
            NQALQNCRELLEYAIDD+ DSF RIG+F+M+KLD+FV D+ IWLS ALTYEQTCLDGFE
Sbjct: 126 TNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTCLDGFE 185

Query: 183 NTTGDSGERMKEFLKSGKEMTSNGLVI-------LGG-SGMKVGRRRLMVNYEEIPSWVS 242
           NTTGD+GERMK FLKS +EMTSNGL I       LG   G K GRR  ++  +E+PSWVS
Sbjct: 186 NTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRR--LLAGDEVPSWVS 245

Query: 243 EGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVI 302
             KR+LMQ   +++ P+VVVA+DGSGKYKTI  AL +VPKKSN TFVIYVKAGIYKETV+
Sbjct: 246 SEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYKETVL 305

Query: 303 VPKALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIK 362
           +PKAL HVTM+GDGPTKT+++G LN+VDG+QTFKTATFA +G NF  +DMGF N+AG+ K
Sbjct: 306 IPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAK 365

Query: 363 HQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCE 422
           HQAVALRVQSDRS+F NC +DGYQDTLY   HRQF+RDCK+SGTIDFVFGN+AAVFQNC 
Sbjct: 366 HQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCN 425

Query: 423 LIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYS 482
           L+VRKPL NQ CIVTAQGRLE REPTG VFQ CH MGD  YLPFKA NKAYLGRPWKQYS
Sbjct: 426 LVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPWKQYS 485

Query: 483 RTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQV 542
           RTIIMGS+IDDLIQP+GWLPW G F L++LFY+EF NKGPGADL+ RV W GIK +T Q 
Sbjct: 486 RTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTLTAQQ 545

Query: 543 AEGFTPRKFMDGDAWIKPTGVPYASGMM 562
           A  FTP+ F+DGDAWI  TGVPY+ GMM
Sbjct: 546 AADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of Sed0017048 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 628.2 bits (1619), Expect = 6.1e-180
Identity = 304/564 (53.90%), Postives = 403/564 (71.45%), Query Frame = 0

Query: 8   QKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAICQP 67
           ++K++  ++++SS++L++MVVAVTVGV+ N       DG+ + K E+ AS KA+K +C P
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNK-----HDGDSKGKAEVNASVKAVKDVCAP 69

Query: 68  TDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPMANQALQ 127
           TDY++TCE +L+    NTTDP EL K    V  +Q+  A + S T+ EL KD     AL 
Sbjct: 70  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 129

Query: 128 NCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTTGDS 187
            C+EL++YA+D+L++SF  +G FE   LDE + +++IWLSAA+++E+TCL+GF+ T G++
Sbjct: 130 QCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNA 189

Query: 188 GERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNYEEIPSWVSEGKRQL 247
           GE MK+ LK+  E+T NGL I+      VG+        RRL+   E  PSWV +  R+L
Sbjct: 190 GETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA--EGFPSWVDQRGRKL 249

Query: 248 MQAGTA--SLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVIVPKA 307
           +QA  A   +KPD+VVA+DGSG+YKTI  AL  VPKK NTTFV+++KAG+YKE V V K 
Sbjct: 250 LQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKT 309

Query: 308 LRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIKHQAV 367
           + H+   GDGP KT+I+GN NY DG+ T++TAT A +G  F+ K++GF N+AGAIKHQAV
Sbjct: 310 MSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAV 369

Query: 368 ALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCELIVR 427
           A+RVQSD S+F NC  DGYQDTLYTH+HRQFFRDC ISGTIDF+FG+AAAVFQNC L+VR
Sbjct: 370 AVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVR 429

Query: 428 KPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYSRTII 487
           KPL NQ C +TA GR + RE TG VFQGC   G+  YL  K  +KAYLGRPWK+YSRTII
Sbjct: 430 KPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTII 489

Query: 488 MGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQVAEGF 547
           M + I D +QPQGW PW G F L TLFYSE  N GPG+ L+ RVTW GIK ++ +    F
Sbjct: 490 MNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKF 549

Query: 548 TPRKFMDGDAWIKPTGVPYASGMM 562
           TP +++ GD WI   GVPY +G++
Sbjct: 550 TPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Sed0017048 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 615.9 bits (1587), Expect = 3.2e-176
Identity = 296/564 (52.48%), Postives = 402/564 (71.28%), Query Frame = 0

Query: 5   EGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAI 64
           E  ++KK+  I+S+SS++L++MVVAVT+GV+ N + + G +       EIT S KAIK +
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDE-------EITTSVKAIKDV 67

Query: 65  CQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTTLQELAKDPMANQ 124
           C PTDYKETCE +L  +A +T+DP EL K       +Q++   + S T+ EL KDP A  
Sbjct: 68  CAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKM 127

Query: 125 ALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTT 184
           AL  C+EL++YAI +L+ SF  +G FE  K+DE +  ++IWLSA +++EQTCLDGF+ T 
Sbjct: 128 ALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQ 187

Query: 185 GDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------RRLMVNYEEIPSWVSEGK 244
           G++GE +K+ LK+  ++T NGL ++      +G+        RRL+   +E PSW+    
Sbjct: 188 GNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL--SQEFPSWMDARA 247

Query: 245 RQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIYKETVIVPK 304
           R+L+ A  + +KPD+VVA+DGSG+YKTI  AL  VPKK NTTFV+++K GIYKE V V +
Sbjct: 248 RRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNR 307

Query: 305 ALRHVTMFGDGPTKTVITGNLNYVDGVQTFKTATFAAIGPNFLGKDMGFVNSAGAIKHQA 364
           ++ H+   GDGP KTVI+G+ +Y DG+ T+KTAT A +G +F+ K++ F N+AGAIKHQA
Sbjct: 308 SMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQA 367

Query: 365 VALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAAVFQNCELIV 424
           VA+RV +D S+F+NC  DGYQDTLY H+HRQF+RDC ISGTIDF+FG+AAAVFQNC L+V
Sbjct: 368 VAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLV 427

Query: 425 RKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGRPWKQYSRTI 484
           RKPL NQ C +TA GR + RE TG V QGC  +G+  YL  K ++K YLGRPWK+YSRTI
Sbjct: 428 RKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTI 487

Query: 485 IMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIKKITPQVAEG 544
           IM + I D + P+GW PW G F L+TLFYSE  N GPGA ++KRVTWPGIKK++ +    
Sbjct: 488 IMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILK 547

Query: 545 FTPRKFMDGDAWIKPTGVPYASGM 561
           FTP +++ GDAWI   GVPY  G+
Sbjct: 548 FTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Sed0017048 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-156
Identity = 269/569 (47.28%), Postives = 375/569 (65.91%), Query Frame = 0

Query: 5   EGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAI 64
           +G+ KKKK  I  V + +LV MVVAV +  ++N +  +          +I  ++ A++A+
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSE-----KIVPVQIKTATTAVEAV 63

Query: 65  CQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNSTT--LQELAKDPMA 124
           C PTDYKETC  SLM  + ++T P +L K+G  V    +  +I+ ++     + A D   
Sbjct: 64  CAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDT 123

Query: 125 NQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFEN 184
             AL+ C +L+  A DDL         F + ++++FV D+++WLS ++ Y+QTC+D FE 
Sbjct: 124 KGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEE 183

Query: 185 TTGDSGERMKEFLKSGKEMTSNGLVILGGSGMKVGR--------------RRLMVNYEEI 244
           T     + M++  K+ +E+TSNGL ++      +G               R+L+   + I
Sbjct: 184 TNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGI 243

Query: 245 PSWVSEGKRQLMQAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYVKAGIY 304
           PSWV    R+LM A    +K +VVVA DGSG+YKTI  AL  VPK +   FVIY+K G+Y
Sbjct: 244 PSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 303

Query: 305 KETVIVPKALRHVTMFGDGPTKTVITGNLNYVDG-VQTFKTATFAAIGPNFLGKDMGFVN 364
            E V V K + HVT  GDGPTKT ITG+LNY  G V+T+ TAT A  G NF  K++GF N
Sbjct: 304 NEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFEN 363

Query: 365 SAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVFGNAAA 424
           +AG   HQAVALRV +D +VF+NC +DGYQDTLY H+HRQFFRDC +SGT+DF+FG+   
Sbjct: 364 TAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIV 423

Query: 425 VFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENKAYLGR 484
           V QNC ++VRKP+++Q+C++TAQGR +KRE TGLV Q CH  G+  Y+P K+ NKAYLGR
Sbjct: 424 VLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGR 483

Query: 485 PWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVTWPGIK 544
           PWK++SRTIIMG+ IDD+I P GWLPW G FAL+TL+Y+E+ N GPG++ ++RV WPGIK
Sbjct: 484 PWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIK 543

Query: 545 KITPQVAEGFTPRKFMDGDAWIKPTGVPY 557
           K++P+ A  FTP +F+ G+ WI P  VPY
Sbjct: 544 KLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Sed0017048 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 513.1 bits (1320), Expect = 2.9e-145
Identity = 262/576 (45.49%), Postives = 374/576 (64.93%), Query Frame = 0

Query: 4   AEGDQKKKKIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKA 63
           ++GD+KKK I   SVS   LV MVV+V V  +K+  KDD        +  I  ++KA++A
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSKHSPKDD--------ENHIRKTTKAVQA 62

Query: 64  ICQPTDYKETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAIRNST--TLQELAKDPM 123
           +C PTD+K+TC  SLM  + ++ DP +L K+G +V  + +N ++  ++     +  K+P 
Sbjct: 63  VCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPE 122

Query: 124 ANQALQNCRELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFE 183
           A  A + C +L+  AIDDL         F + +++ FV D+++WLS ++ ++QTC+D F 
Sbjct: 123 AKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFG 182

Query: 184 NTTGDSGERMKEFLKSGKEMTSNGLVI---------------LGGSGMKVGRRRLMVNYE 243
               +  + M +  K+ +E++SN L +               L G+  K   R+L+   +
Sbjct: 183 EIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA-RKLLSTED 242

Query: 244 EIPSWVSEGKRQLMQA---GTASLKPDVVVAKDGSGKYKTITAALAEVPKKSNTTFVIYV 303
            IP+WV    R+LM A   G   +K + VVA+DG+G++KTIT AL  VPK +   F+I++
Sbjct: 243 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHI 302

Query: 304 KAGIYKETVIVPKALRHVTMFGDGPTKTVITGNLNY-VDGVQTFKTATFAAIGPNFLGKD 363
           K GIYKE V V K + HVT  GDGP KT+ITG+LN+ +  V+TF TAT    G +F  K+
Sbjct: 303 KEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKN 362

Query: 364 MGFVNSAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKISGTIDFVF 423
           +G  N+AG    QAVALRV +D +VFH+C +DG+QDTLY H+HRQF+RDC +SGT+DF+F
Sbjct: 363 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 422

Query: 424 GNAAAVFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGYLPFKAENK 483
           G+A  + QNC+++VRKP + Q C+VTAQGR   RE TGLV  GCH  GD  Y+P K+ NK
Sbjct: 423 GDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNK 482

Query: 484 AYLGRPWKQYSRTIIMGSQIDDLIQPQGWLPWTGTFALDTLFYSEFNNKGPGADLSKRVT 543
           AYLGRPWK++SRTIIM + IDD+I P GWLPW+G FAL TL+Y+E  N GPG++ ++RV 
Sbjct: 483 AYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVK 542

Query: 544 WPGIKKITPQVAEGFTPRKFMDGDAWIKPTGVPYAS 559
           WPGIKK+TPQ A  +T  +F+ GD WI  T VPY +
Sbjct: 543 WPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Sed0017048 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 443.0 bits (1138), Expect = 3.7e-124
Identity = 263/702 (37.46%), Postives = 371/702 (52.85%), Query Frame = 0

Query: 12  KIAIVSVSSLILVAMVVAVTVGVNKNPAKDDGKDGNGEAKPEITASSKAIKAICQPTDYK 71
           K  ++ V++LI+ AMV+ V      +P++   ++ + E+K  IT ++ +I  IC  TDYK
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSPSR-KMEEVHRESKLMITKTTVSI--ICASTDYK 62

Query: 72  ETCEKSLMSEAGNTTDPKELAKVGLRVATEQLNSAI-RNSTTLQELA-KDPMANQALQNC 131
           + C  SL +    + DP+ L +    +A   + S I R    L+  A  D    +AL  C
Sbjct: 63  QDCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTC 122

Query: 132 RELLEYAIDDLNDSFARIGSFEMSKLDEFVNDIKIWLSAALTYEQTCLDGFENTTGDSGE 191
           REL++ AIDDL  +  +   F  ++L +FV D+ +WLS ++TY+QTC+DGFE    ++  
Sbjct: 123 RELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 192 RMKEFLKSGKEMTSNGLVI------------------------LG--GSGM----KVG-- 251
            M+  ++ G+ +TSNGL I                        LG  GSG      VG  
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 252 ------------------------------------------------------------ 311
                                                                       
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 312 ------------------------------------------------------------ 371
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 372 RRRLMVNYEEIPSWVSEGKRQLM--QAGTASLKPDVVVAKDGSGKYKTITAALAEVPKKS 431
           + R +    E P WV+   R+L+  +     +K +VVVAKDGSGK KTI  ALA VP K+
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKN 422

Query: 432 NTTFVIYVKAGIYKETVIVPKALRHVTMFGDGPTKTVITGNLNYV-DGVQTFKTATFAAI 491
              FVI++K G+YKE V V K + HV   GDGPTKTVITG++ ++ D V T++TA+ A  
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 492 GPNFLGKDMGFVNSAGAIKHQAVALRVQSDRSVFHNCYVDGYQDTLYTHAHRQFFRDCKI 551
           G  F+ KD+GF N+AGA +HQAVALRV +D +VF NC+++GYQDTLY H HRQF+R+C++
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 552 SGTIDFVFGNAAAVFQNCELIVRKPLENQNCIVTAQGRLEKREPTGLVFQGCHFMGDRGY 557
           SGTIDFVFG+A AVFQNCE ++R+P+E+Q CIVTAQGR ++RE TG+V       GD  Y
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602