Sed0017012 (gene) Chayote v1

Overview
NameSed0017012
Typegene
OrganismSechium edule (Chayote v1)
DescriptionrRNA N-glycosidase
LocationLG07: 434899 .. 436779 (-)
RNA-Seq ExpressionSed0017012
SyntenySed0017012
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTTGTTACTCTAATTGCCACTCATGGCATTGAAGCCTACATCTGTTTGTCACTGTCTTCCTCCTCAAATTCATTTGCTGACGATTACCGAAGGTTAATTCGTGCGATATGAAGGAACCTAATTGCCAAAACCCATCAATTATACAGTATCACTCTAGCAATTGACGTAACGAATGCCGGTTTTATTGCATATCGGGCAAACAACATATCCTATATATTTCAAGTCGTCTGCCCCACTGTTGCCTCTCGGGTTTTTCTGGATTTCAAGGGTGACTATGAATCCATGGAGGGTGCAGCTGGTATTACAAGAATGGAAACTTTCCTTGGATTGGGTGCTTTCGATAGAGCCATCTCCAATTGGTTTGACTACAATCAAGAATATCTCCCTGAATCTTTTCTTTTGTTCACTCAAATGTTGTTGGAACCTTCCAAGTTTAAATTCATGAGCAGAGTATTGTCAATATGCCAAGGAATCGTCGTATCTTCTATCCTAGACTTGCAATGTTGAGCTTGGAGAACAATTGGGTAAAACATTCTTTCACAAATCTAAGCATCAGTAACACTACAAGGAGTATTTGGAACAATCGTTGATCTTTACGATTCAAATGATGAAATCATTCTAGTTGATAGCATACATTATCCAATCATAACAACCAACATTGCTTTTATGTTGTATCGTTGCAAGGATGATGCGACAAACACAATAATAAGAATGCCTAGTGATGTGAGTGATAGTGATAGTGATAGTGATTCATGCTATGTTGGGAGGCTAAGGCGTAGAACACGCATTACTAGGCGGGAAGGCTTGTGCATGGATATAGATAATAAAGATTATTTTGAGGACAATTCTATCATTTTGAATCCATGTAAAGAACAAGTGAATAAAAAATGGAGATTTCAAAATGCCGGGACTATTCAAGCCTTTTTGGGTACTTGCTTGACCTTTAATCTTGAAAACTCCATCATTATTTTGAATTGTAGTGAGTGTTGAAGATATGCCTGGAACGCATTATGTTGTGTGTTCCACGTGTGCTAGTGCATCCACATTCTGTTTAATGCTTACGCATTCTGTAATAGCCGTATTATGACATTCATTCTGTAATTTCTGTAATTTTGTTCTCCTCTCCTTAGTAAAACATGGCCCCCTCTCTGCTCGTGGACTAGCTAACATTATGTTAGTGAACCATGTAAACTTTCTGTGTGTTTGTTCTTTTTTTCTCACGTTTCTTTATTCTTTGTTTTACGATTGACGTTGATTGTTGTTTTCATAACCGTAAGGTTAAGCAAGCACCACTAAATGGAAAGTTTCCCTTGATGGGACTATCACCAACCCGGAGTCTGGCTTTGTGCTTACAGCAAATGCATCAACAAGCAGAACAAAGTTATCAGTAGCGATAGACAGCAAGGCATTTGGGCAAGGCTGGAGAGTGGGAAACTATGTAAAGCCAATTACAACTTCTATTATAGGGTTGCAAGGGATGTGCCTTTGAGGCAACAAATAAGAACACAAACGCATGGTTGGAAAAATGCGTGAAGAATTAGAAAGATCAAAGTTGGGCAGTTTATGGCGATAAGAGTAAACAACGATCGTAGTTTGTGTGTGTCATCCCCAACAGATGGCTCAGCGGGGCTTGTTTCGATGCTTAGCTGCATGGCTGGATCCACTAAACAACGCTGGCTTATCATGGCGAATGGCACAATCTTGAACCCTAAAACAGGGATGGCGTTGTTGAAAGAGGCAATACGAGAATTATTTTTGCAGCCTACAACGGTAAAGCTGAGCAAGAATGGGCGTTATTGTACTAATTCGTTTAGGTTGATTCACACATTATGGTGTGTTAATGGATGTACTCCATGCAAATGTATCCGCCTCTAA

mRNA sequence

ATGGTTGTTGTTACTCTAATTGCCACTCATGGCATTGAAGCCTACATCTGTTTGTCACTGTCTTCCTCCTCAAATTCATTTGCTGACGATTACCGAAGTATCACTCTAGCAATTGACGTAACGAATGCCGGTTTTATTGCATATCGGGCAAACAACATATCCTATATATTTCAAGTCGTCTGCCCCACTGTTGCCTCTCGGGTTTTTCTGGATTTCAAGGGTGACTATGAATCCATGGAGGGTGCAGCTGGTATTACAAGAATGGAAACTTTCCTTGGATTGGGTGCTTTCGATAGAGCCATCTCCAATTGGTTTGACTACAATCAAGAATATCTCCCTGAATCTTTTCTTTTGTTCACTCAAATGTTGTTGGAACCTTCCAAGTTTAAATTCATGAGCAGAGTATTGTCAATATGCCAAGGAATCGTCGTATCTTCTATCCTAGACTTGCAATCATCAGTAACACTACAAGGAGTATTTGGAACAATCGTTGATCTTTACGATTCAAATGATGAAATCATTCTAGTTGATAGCATACATTATCCAATCATAACAACCAACATTGCTTTTATGTTGTATCGTTGCAAGGATGATGCGACAAACACAATAATAAGAATGCCTAGTGATGTGAGTGATAGTGATAGTGATAGTGATTCATGCTATGTTGGGAGGCTAAGGCGTAGAACACGCATTACTAGGCGGGAAGGCTTGTGCATGGATATAGATAATAAAGATTATTTTGAGGACAATTCTATCATTTTGAATCCATGTAAAGAACAAGTGAATAAAAAATGGAGATTTCAAAATGCCGGGACTATTCAAGCCTTTTTGGGTACTTGCTTGACCTTTAATCTTGAAAACTCCATCATTATTTTGAATTGTAGTGAGTCAAGCACCACTAAATGGAAAGTTTCCCTTGATGGGACTATCACCAACCCGGAGTCTGGCTTTGTGCTTACAGCAAATGCATCAACAAGCAGAACAAAGTTATCAGTAGCGATAGACAGCAAGGCATTTGGGCAAGGCTGGAGAGTGGGAAACTATGTAAAGCCAATTACAACTTCTATTATAGGGTTGCAAGGGATAAAGATCAAAGTTGGGCAGTTTATGGCGATAAGAGTAAACAACGATCGTAGTTTGTGTGTGTCATCCCCAACAGATGGCTCAGCGGGGCTTGTTTCGATGCTTAGCTGCATGGCTGGATCCACTAAACAACGCTGGCTTATCATGGCGAATGGCACAATCTTGAACCCTAAAACAGGGATGGCGTTGTTGAAAGAGGCAATACGAGAATTATTTTTGCAGCCTACAACGGTAAAGCTGAGCAAGAATGGGCGTTATTGTACTAATTCGTTTAGGTTGATTCACACATTATGGTGTGTTAATGGATGTACTCCATGCAAATGTATCCGCCTCTAA

Coding sequence (CDS)

ATGGTTGTTGTTACTCTAATTGCCACTCATGGCATTGAAGCCTACATCTGTTTGTCACTGTCTTCCTCCTCAAATTCATTTGCTGACGATTACCGAAGTATCACTCTAGCAATTGACGTAACGAATGCCGGTTTTATTGCATATCGGGCAAACAACATATCCTATATATTTCAAGTCGTCTGCCCCACTGTTGCCTCTCGGGTTTTTCTGGATTTCAAGGGTGACTATGAATCCATGGAGGGTGCAGCTGGTATTACAAGAATGGAAACTTTCCTTGGATTGGGTGCTTTCGATAGAGCCATCTCCAATTGGTTTGACTACAATCAAGAATATCTCCCTGAATCTTTTCTTTTGTTCACTCAAATGTTGTTGGAACCTTCCAAGTTTAAATTCATGAGCAGAGTATTGTCAATATGCCAAGGAATCGTCGTATCTTCTATCCTAGACTTGCAATCATCAGTAACACTACAAGGAGTATTTGGAACAATCGTTGATCTTTACGATTCAAATGATGAAATCATTCTAGTTGATAGCATACATTATCCAATCATAACAACCAACATTGCTTTTATGTTGTATCGTTGCAAGGATGATGCGACAAACACAATAATAAGAATGCCTAGTGATGTGAGTGATAGTGATAGTGATAGTGATTCATGCTATGTTGGGAGGCTAAGGCGTAGAACACGCATTACTAGGCGGGAAGGCTTGTGCATGGATATAGATAATAAAGATTATTTTGAGGACAATTCTATCATTTTGAATCCATGTAAAGAACAAGTGAATAAAAAATGGAGATTTCAAAATGCCGGGACTATTCAAGCCTTTTTGGGTACTTGCTTGACCTTTAATCTTGAAAACTCCATCATTATTTTGAATTGTAGTGAGTCAAGCACCACTAAATGGAAAGTTTCCCTTGATGGGACTATCACCAACCCGGAGTCTGGCTTTGTGCTTACAGCAAATGCATCAACAAGCAGAACAAAGTTATCAGTAGCGATAGACAGCAAGGCATTTGGGCAAGGCTGGAGAGTGGGAAACTATGTAAAGCCAATTACAACTTCTATTATAGGGTTGCAAGGGATAAAGATCAAAGTTGGGCAGTTTATGGCGATAAGAGTAAACAACGATCGTAGTTTGTGTGTGTCATCCCCAACAGATGGCTCAGCGGGGCTTGTTTCGATGCTTAGCTGCATGGCTGGATCCACTAAACAACGCTGGCTTATCATGGCGAATGGCACAATCTTGAACCCTAAAACAGGGATGGCGTTGTTGAAAGAGGCAATACGAGAATTATTTTTGCAGCCTACAACGGTAAAGCTGAGCAAGAATGGGCGTTATTGTACTAATTCGTTTAGGTTGATTCACACATTATGGTGTGTTAATGGATGTACTCCATGCAAATGTATCCGCCTCTAA

Protein sequence

MVVVTLIATHGIEAYICLSLSSSSNSFADDYRSITLAIDVTNAGFIAYRANNISYIFQVVCPTVASRVFLDFKGDYESMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVLSICQGIVVSSILDLQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSESSTTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQGIKIKVGQFMAIRVNNDRSLCVSSPTDGSAGLVSMLSCMAGSTKQRWLIMANGTILNPKTGMALLKEAIRELFLQPTTVKLSKNGRYCTNSFRLIHTLWCVNGCTPCKCIRL
Homology
BLAST of Sed0017012 vs. NCBI nr
Match: XP_038884101.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 317.4 bits (812), Expect = 2.1e-82
Identity = 196/461 (42.52%), Postives = 277/461 (60.09%), Query Frame = 0

Query: 13  EAYICLSLSSSSNSFADDYRSITLAIDVTNAGFIAYRANNISYIF--------QVVCPTV 72
           E +I ++L +++N       +I LAIDV N G + YR+NN SY+F        ++V P+ 
Sbjct: 69  EPFITINLKNANN------ETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFPST 128

Query: 73  ASRVFLDFKGDYESMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLL 132
             RV L F  DYES+E A+GI+R++T LG    + AISN F Y+QE LP+SFL+  QM+L
Sbjct: 129 C-RVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVL 188

Query: 133 EPSKFKFM--SRVLSICQG------IVVSSI--------LDLQSSVTLQGVFGTIVDLYD 192
           E  KFKF+  S + S+  G      + + S+        L +Q+S +LQG+FG  ++ YD
Sbjct: 189 EGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYD 248

Query: 193 SNDEIILVDSIHYPIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRR 252
           SN+ II VDSI+YPII TNIA  LY+C  + +   IRMPS  SDS      CY+    + 
Sbjct: 249 SNNRIIQVDSIYYPIIITNIALQLYQC--NVSTNFIRMPSITSDS-----PCYI--QTQT 308

Query: 253 TRITRREGLCMDIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENS 312
           + I+ REG C+D      ++ N II +PC+ Q N+KW FQ  GTI+ +   CLTF+    
Sbjct: 309 SSISGREGFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIR-YSNKCLTFDTSRF 368

Query: 313 IIILNCS--ESSTTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVG 372
           +++ NCS  E+ +TKW V++DGTI+NP SG VLTAN+STS T+L V  +    GQGWRVG
Sbjct: 369 VVLYNCSKVEAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVG 428

Query: 373 NYVKPITTSIIGLQGIKI-----------------KVGQFMA------IRVNNDRSLCVS 425
           NYV+PI  SIIGL+ + +                 K  Q+ A      IRVN++R+LCV+
Sbjct: 429 NYVEPIIGSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVT 488

BLAST of Sed0017012 vs. NCBI nr
Match: XP_038877566.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 307.0 bits (785), Expect = 2.8e-79
Identity = 194/458 (42.36%), Postives = 264/458 (57.64%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYI--------FQVVCPTVASRVFLDFKGDYESMEGAAG 92
           +ITLAID  N G + Y +NNISY+        F+VV P+   RV LDF  DY+S+E AA 
Sbjct: 82  TITLAIDKVNLGVVGYLSNNISYMFLNAPREAFRVVFPSTC-RVILDFDSDYKSIEIAAS 141

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVL-------- 152
            TR E  LG    D AIS  F Y +  +P++FL+  QM++E  KFKF+ + +        
Sbjct: 142 TTRQEIVLGFDPMDVAISTLFHYQENSIPKAFLVILQMVIEGLKFKFIEQSVFMSLKYRY 201

Query: 153 SICQGIVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
           +    + + S+ D        +Q+S +LQG+FG +++LYDSND+II VD+I YPII TNI
Sbjct: 202 NFKPSLAIVSLQDNWVRLSSQIQASSSLQGLFGEVIELYDSNDKIIRVDAIDYPIILTNI 261

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           A  LY C  + +  II+M          +DSCY+      TRI+ R GLC D+ N    +
Sbjct: 262 AMQLYHC--NVSTNIIKM-------TVQNDSCYI--QSHSTRISGRAGLCADVKNGFSND 321

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE--SSTTKWKVSL 332
            N IIL PC +Q N+ W FQ  GTIQ+ LG CLTFN  N ++I NCS+   ST KWKVS+
Sbjct: 322 GNPIILYPCGQQANQNWTFQRDGTIQS-LGKCLTFNDTNFVVIYNCSKVAPSTIKWKVSI 381

Query: 333 DGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQG----- 392
           DGTI++P SG VLTAN+  + TKL +  +++ F Q WRVGNY++PI  SIIG  G     
Sbjct: 382 DGTISHPSSGLVLTANSPAANTKLKMEANTQTFSQCWRVGNYIEPIVGSIIGFYGVCLEA 441

Query: 393 ------------IKIKVGQFMA------IRVNNDRSLCVSSPTDG--SAGLVSMLSCMAG 439
                       +K K  Q+ A      IRVN+ R+LCVSS +       +V++L C  G
Sbjct: 442 TNDNTNLKLADCVKNKRQQYWALYGDGTIRVNSSRNLCVSSSSSDCIPGRVVTVLKCNGG 501

BLAST of Sed0017012 vs. NCBI nr
Match: XP_008445788.1 (PREDICTED: nigrin b-like [Cucumis melo] >KAA0033146.1 nigrin b-like [Cucumis melo var. makuwa] >TYK17088.1 nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 301.2 bits (770), Expect = 1.6e-77
Identity = 189/442 (42.76%), Postives = 261/442 (59.05%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVA--------SRVFLDFKGDYESMEGAAG 92
           +I+LAID  N G + YR+NN SYIF +  P VA         RV L F  +YES+E A+G
Sbjct: 82  TISLAIDKVNLGVLGYRSNNTSYIF-LDAPLVAFDIVFPKTCRVLLGFNSNYESIEKASG 141

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVL-------- 152
            TR++T LGL   + AISN F Y++  +PESFL+  QM+LE SKFKF+ R +        
Sbjct: 142 TTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLEGSKFKFIERSVIQSLKYGY 201

Query: 153 SICQGIVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
           +   G+ + S+ D        +Q+S +LQG+FG  + LYDSN+++I VDSI+Y IITTNI
Sbjct: 202 NFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDSNNKVIEVDSIYYAIITTNI 261

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           AF L+ C  + +   IRMPSDV       DSC V    R   IT + G C+D  N  + +
Sbjct: 262 AFQLHHC--NISTNFIRMPSDV------GDSCNV--QTRTATITGQNGFCVDASNGLHQD 321

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE---SSTTKWKVS 332
           DNSIIL  C  Q N++W F +  TI+ +   CLTF+    +++ NCSE    S  +W V+
Sbjct: 322 DNSIILYRCGYQSNQEWTFLSDKTIR-YSNKCLTFDTSRFVVLYNCSEVEKKSNIRWNVA 381

Query: 333 LDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQG---- 392
           +DGTI+NP SG VLT N ST+ ++L V ++     QGWRVGNYV+P+T SIIGL+     
Sbjct: 382 IDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTGSIIGLEEMCLE 441

Query: 393 -------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSMLSCMAGS 425
                        +K K  Q+ A      IRVN+ R+LCVSS ++    L+ +  C  G+
Sbjct: 442 ATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEEC-NGT 501

BLAST of Sed0017012 vs. NCBI nr
Match: XP_023547577.1 (seed lectin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296.6 bits (758), Expect = 3.8e-76
Identity = 183/453 (40.40%), Postives = 266/453 (58.72%), Query Frame = 0

Query: 25  NSFADDYRSITLAIDVTNAGFIAYRANNISYI--------FQVVCPTVASRVFLDFKGDY 84
           N + +D   I +A+DV     + YR+N+ SY+        F++V P+   RV L F  D+
Sbjct: 77  NPYGED---IFVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFEIVFPSTC-RVLLGFNSDF 136

Query: 85  ESMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFM--SR 144
           ES+E AAG +R++T LGL  F+ A+S+ F Y ++ +P SFL+  QMLLE +KFKF+  S 
Sbjct: 137 ESIENAAGTSRLDTLLGLAPFESAVSDLFHYRRDSVPTSFLVIIQMLLEATKFKFIEQSV 196

Query: 145 VLSICQGI------VVSSI--------LDLQSSVTLQGVFGTIVDLYDSNDEIILVDSIH 204
           V S+  G+       + S+        L +Q+S +LQG+FG  ++LYDSN  II VDSI+
Sbjct: 197 VNSLKNGVPFKPTLAILSLQDNWAKLSLQIQASSSLQGLFGEPIELYDSNQRIIQVDSIY 256

Query: 205 YPIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMD 264
           YPII  ++A  LY+C  +     IRMPS         D CYV    R  RI+  EG C++
Sbjct: 257 YPIIIASMALQLYQC--NIGTNFIRMPS------VGGDPCYV--QTRTARISGLEGFCIE 316

Query: 265 IDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE--SS 324
            +NK + + N +IL+ C  Q+ ++W FQ+ G I  + G CL+FNL + ++  NCSE  + 
Sbjct: 317 AENKGWSDGNPVILSQCGGQLTQRWSFQSDGRI-GYSGKCLSFNLSSYVVTYNCSEALAG 376

Query: 325 TTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIG 384
           + +WKVS++G I+NP S  VLTANAS     L+   ++K  GQ WRVGNYV PI  SIIG
Sbjct: 377 SIRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVGSIIG 436

Query: 385 LQG-----------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSM 429
           L+                  ++ K  Q+ A      IRVN+ R+ CVS+ +D   GL+++
Sbjct: 437 LEETCLESMDNNAKVWLEKCVEQKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITI 496

BLAST of Sed0017012 vs. NCBI nr
Match: KAG6575323.1 (hypothetical protein SDJN03_25962, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 296.2 bits (757), Expect = 5.0e-76
Identity = 182/448 (40.62%), Postives = 264/448 (58.93%), Query Frame = 0

Query: 25  NSFADDYRSITLAIDVTNAGFIAYRANNISYIF----QVVCPTV---ASRVFLDFKGDYE 84
           N + +D   I +A+DV     + YR+N+ SY+F    +V   TV     RV L F  D+E
Sbjct: 77  NPYGED---ILVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFETVFPSTCRVLLGFNSDFE 136

Query: 85  SMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFM--SRV 144
           S+E AA  +R++T LGL  F+ A+S+ F Y ++ +P SFL+  QMLLE +KFK +  S V
Sbjct: 137 SIENAAATSRLDTLLGLAPFEAAVSDLFHYRRDSVPTSFLVIIQMLLEATKFKLIEQSVV 196

Query: 145 LSICQGI------VVSSI--------LDLQSSVTLQGVFGTIVDLYDSNDEIILVDSIHY 204
            S+  G+       + S+        L +Q+S +LQG+FG  ++LYDSN+ II VDSI+Y
Sbjct: 197 NSLKNGVPFKPTLAILSLQDNWAKLSLQIQASSSLQGLFGEPIELYDSNNRIIQVDSIYY 256

Query: 205 PIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDI 264
           PII  N+A  LY+C  +     IRMPS         D CYV    R  RI+  +G C++ 
Sbjct: 257 PIIIANMALQLYQC--NIGTNFIRMPS------VGGDPCYV--QTRTARISGLDGFCVEA 316

Query: 265 DNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE--SST 324
            NK + + N +IL+PC EQ+ ++W FQ+ G I  + G CL+FNL + ++  NCSE  + +
Sbjct: 317 QNKGWSDGNPVILSPCGEQLTQRWSFQSDGRI-GYSGKCLSFNLSSYVVTYNCSEGAAGS 376

Query: 325 TKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGL 384
            +WKVS++G I+NP S  VLTANAS     L+   ++K  GQ WRVGNYV PI  SIIGL
Sbjct: 377 IRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVGSIIGL 436

Query: 385 QG-----------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSML 425
           +                  ++ K  Q+ A      IRVN+ R+ CVS+ +D   GL+++ 
Sbjct: 437 EETCLESMDNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITIT 496

BLAST of Sed0017012 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 4.9e-58
Identity = 162/462 (35.06%), Postives = 252/462 (54.55%), Query Frame = 0

Query: 19  SLSSSSNSF-----ADDYRSITLAIDVTNAGFIAYR--ANNISYIFQVVCPTVASRVF-- 78
           SLS+S+  +     ++   SITLAIDV +   +AY+   ++ SY F +  P +A      
Sbjct: 43  SLSNSNRFYLLTLTSNQDESITLAIDVEDMVAVAYQPAGSHESYFF-LNAPQIAFHTLFT 102

Query: 79  ------LDFKGDYESMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQML 138
                 L+F   ++S+E AAG TR    LG+   D AISN F+ + + LP SFL+  QM+
Sbjct: 103 DTHQNVLNFDNTFKSLENAAGTTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMV 162

Query: 139 LEPSKFKFMSRVL--------SICQGIVVSSI--------LDLQSSVTLQGVFGTIVDLY 198
           LE SKF+F+ + +        +    + + S+        L +Q+S +LQG+FG++V+LY
Sbjct: 163 LEASKFRFIEQSVAYSFKNEKTFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELY 222

Query: 199 DSNDEIILVDSIHYPIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRR 258
           +SN+E+I VDSI+YPII  N+A  LY C+             VS  D++   C V    R
Sbjct: 223 NSNNELIEVDSIYYPIILANVALQLYHCQ-------------VSTGDNE---CLVE--TR 282

Query: 259 RTRITRREGLCMDIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFN--- 318
            TRI+ R+ LC+D+      + + +IL PC +QVN+KW F + GT+++ LG CL  N   
Sbjct: 283 TTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQKWTFHSDGTVRS-LGKCLATNNSK 342

Query: 319 LENSIIILNCSE--SSTTKWKVSLDGTITNPE-SGFVLTANASTSRTKLSVAIDSKAFGQ 378
             N ++I +CS+  +    W VS+ GTI NP      LT+N +T  T L++ +++ +  Q
Sbjct: 343 FGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTYSASQ 402

Query: 379 GWRVGNYVKPITTSIIGLQGIKIKVGQFM-----------------------AIRVNNDR 419
           GWRVGNYV+PI  SI+GL  + ++                             IRV+++R
Sbjct: 403 GWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQREQSWALYSDGTIRVDDNR 462

BLAST of Sed0017012 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 149/465 (32.04%), Postives = 231/465 (49.68%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVASRVF-------LDFKGDYESMEGAAGI 92
           ++TLA+DVTN   +A+  N  SY F+       S +F       L F G+Y+++E AA  
Sbjct: 91  TVTLAVDVTNLYVVAFSGNANSYFFKDATEVQKSNLFVGTKQNTLSFTGNYDNLETAANT 150

Query: 93  TRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKF--------MSRVLS 152
            R    LG    D AI++   Y+ + +  S L+  QM+ E ++F++        + +  S
Sbjct: 151 RRESIELGPSPLDGAITSL--YHGDSVARSLLVVIQMVSEAARFRYIEQEVRRSLQQATS 210

Query: 153 ICQGIVVSSILDLQSSVTLQ---------GVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
                ++ S+ +  SS++L+           FGT V L + +    LVD+       T I
Sbjct: 211 FTPNALMLSMENNWSSMSLEIQQAGNNVSPFFGT-VQLLNYDHTHRLVDNFEELYKITGI 270

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSD---SDSDSCYVGRLRRRTRITRREGLCMDIDNKD 272
           A +L+RC   + +  IRMP D++  D   +D ++C + R      I  R+GLC+D+ N  
Sbjct: 271 AILLFRCSSPSNDNAIRMPLDLAGEDNKYNDGETCTL-RTSFTRNIVGRDGLCVDVRNGY 330

Query: 273 YFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFN-LEN--SIIILNCSES--STT 332
             +   + L PC  Q N++W F +  TI++ +G C+T N L N  +I+I NCS +  +  
Sbjct: 331 DTDGTPLQLWPCGTQRNQRWTFDSDDTIRS-MGKCMTANGLNNGSNIVIFNCSTAAENAI 390

Query: 333 KWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIG-- 392
           KW+V +DG+I NP SG V+TA  + SRT L +  +  A  QGW V N VKPI  SI+G  
Sbjct: 391 KWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYAASQGWTVTNNVKPIVASIVGYK 450

Query: 393 ---LQGIKIKVGQFM------------------AIRVNNDRSLCVSSPTDGSAGLVSMLS 439
              LQ      G +M                   IRVN+ R LCV++    S  L+ +L 
Sbjct: 451 EMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRVNSTRGLCVTTNGYNSKDLIIILK 510

BLAST of Sed0017012 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.3e-42
Identity = 143/453 (31.57%), Postives = 219/453 (48.34%), Query Frame = 0

Query: 32  RSITLAIDVTNAGFIAYRANNISYIFQVVCPTVASRVFLD-------FKGDYESMEGAAG 91
           + +TLAIDV     +A+  N+ SY F          +F+D       FKGDY S+E   G
Sbjct: 96  KKVTLAIDVVTFYVVAFAQNDRSYFFSGSSEVQRENLFVDTTQEDLNFKGDYTSLEHQVG 155

Query: 92  ITRMETFLGLGAFDRAISNWFDY-----NQEYLPESFLLFTQMLLEPSKFKF-------- 151
             R+   LG  +  ++IS+   Y     + + L  S L+  QM+ E ++F++        
Sbjct: 156 FGRVYIPLGPKSLAQSISSLSTYKSSAGDNKRLARSLLVVIQMVSEAARFRYIQLRIQAS 215

Query: 152 MSRVLSICQGIVVSSILDLQSSVTLQ-------GVFGTIVDLYDSNDEIILVDSIHYPII 211
           ++        +++ S+ +  SS++ +       G F  +V L D  +  I V +      
Sbjct: 216 ITDAKEFTPDLLMLSMENKWSSMSSEIQQAQPGGAFAQVVKLLDQRNHPIDVTNFRRLFQ 275

Query: 212 TTNIAFMLYRCKDDATNTIIRMPS-----DVSDSDSDSDSCYVGRLRRRTRITRREGLCM 271
            T++A +L+ C      T+ +MP+      V +   D + C V     R RI  R+G C 
Sbjct: 276 LTSVAVLLHGCP-----TVTKMPAYIIKMPVFNGGEDEERCSVVEEVTR-RIGGRDGFCA 335

Query: 272 DIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCS--ES 331
           ++ N D  +   + L+ C EQ N++W F   GTIQ+ LG CLT    +S++I NC     
Sbjct: 336 EVKNGDEKDGTPVQLSSCGEQSNQQWTFSTDGTIQS-LGKCLT--TSSSVMIYNCKVVPP 395

Query: 332 STTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSII 391
            +TKW VS+DGTITNP SG VLTA  +   T +S+  +  A  QGW VGN V+P+ T I+
Sbjct: 396 ESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQGWIVGN-VEPLVTFIV 455

Query: 392 GLQGIKI--------------------KVGQFMA------IRVNNDRSLCVSSPTDGSAG 425
           G + + +                    KV Q  A      IRVNNDRSLCV+S    S  
Sbjct: 456 GYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNE 515

BLAST of Sed0017012 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.1e-41
Identity = 146/464 (31.47%), Postives = 227/464 (48.92%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVASRVFLD-------FKGDYESMEGAAGI 92
           ++TLAIDV N   +A+ +N  SY F          +F+D       F G+Y S+E   G 
Sbjct: 95  TVTLAIDVVNLYVVAFSSNGKSYFFSGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGF 154

Query: 93  TRMETFLGLGAFDRAISNWFDY-----NQEYLPESFLLFTQMLLEPSKFKF--------M 152
            R+   LG  + D+AIS+   Y     + + L    L+  QM+ E ++F++        +
Sbjct: 155 GRVYIPLGPKSLDQAISSLRTYTLTAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSI 214

Query: 153 SRVLSICQGIVVSSILDLQSSVTLQ-------GVFGTIVDLYDSNDEIILVDSIHYPIIT 212
           +        +++ S+ +  SS++ +       G+F  +V L D  +  I V +       
Sbjct: 215 TDASEFTPDLLMLSMENNWSSMSSEIQQAQPGGIFAGVVQLRDERNNSIEVTNFRRLFEL 274

Query: 213 TNIAFMLYRCKDDATNT---------IIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREG 272
           T IA +LY C    +++         II+MP  V         C V  + R  RI+  +G
Sbjct: 275 TYIAVLLYGCAPVTSSSYSNNAIDAQIIKMP--VFRGGEYEKVCSVVEVTR--RISGWDG 334

Query: 273 LCMDIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCS- 332
           LC+D+    Y + N + L PC  + N+ W F+  GTI+ +LG CLT    +S++I +C+ 
Sbjct: 335 LCVDVRYGHYIDGNPVQLRPCGNECNQLWTFRTDGTIR-WLGKCLT--ASSSVMIYDCNT 394

Query: 333 -ESSTTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITT 392
                TKW VS+DGTITNP SG VLTA  +   T LS+  +  A  QGW VG+ V+P+ T
Sbjct: 395 VPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVT 454

Query: 393 SIIGLQGIKI-----------------KVGQFMA------IRVNNDRSLCVSSPTDGSAG 436
            I+G + + +                 +V Q  A      IRVN++RSLCV+S     + 
Sbjct: 455 FIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSD 514

BLAST of Sed0017012 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.4e-41
Identity = 147/471 (31.21%), Postives = 228/471 (48.41%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVASRVFLD-------FKGDYESMEGAAGI 92
           ++TLAIDV N   +A+ +N  SY F          +F+D       F G+Y S+E   G 
Sbjct: 95  TVTLAIDVVNLYVVAFSSNGRSYFFSGSTAVQRDNLFVDTTQEELNFTGNYISLERQVGF 154

Query: 93  TRMETFLGLGAFDRAISNWFDY-----NQEYLPESFLLFTQMLLEPSKFKF--------M 152
            R+   LG  +  +AIS+   Y     + + L    L+  QM+ E ++F++        +
Sbjct: 155 GRVYIPLGPKSLAQAISSLRTYTLSAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSI 214

Query: 153 SRVLSICQGIVVSSILDLQSSVTLQ-------GVFGTIVDLYDSNDEIILVDSIHYPIIT 212
           +        +++ S+ +  SS++ +       G+F  +V L D  +  I V +       
Sbjct: 215 TDASEFTPDLLMLSMENNWSSMSSEIQQAQPGGIFPGVVQLRDERNNPIEVTNFRRLFEL 274

Query: 213 TNIAFMLYRCKDDATNT---------IIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREG 272
           T IA +LY C    +N+         II+MP  V         C V  + R  RI+  +G
Sbjct: 275 TYIAVLLYGCAPVTSNSYTNNAIDAQIIKMP--VFRGGGYEKVCSVVEVTR--RISGWDG 334

Query: 273 LCMDIDNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCS- 332
           LC+D+ +  Y + N++ L PC  + N+ W F+  GTI+ +LG CLT    +S++I +C+ 
Sbjct: 335 LCVDVRDGHYIDGNTVQLGPCGNECNQLWTFRTDGTIR-WLGKCLT--TSSSVMIYDCNT 394

Query: 333 -ESSTTKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITT 392
                TKW VS DGTITNP SG VLTA  +   T LS+  +  A  QGW VG+ V+P+ T
Sbjct: 395 VPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVT 454

Query: 393 SIIGLQGIKI-----------------KVGQFMA------IRVNNDRSLCVSSPTDGSAG 439
            I+G + + +                 +V Q  A      IRVN++RSLCV+S     + 
Sbjct: 455 FIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSD 514

BLAST of Sed0017012 vs. ExPASy TrEMBL
Match: A0A5D3D0Q4 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00170 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 7.5e-78
Identity = 189/442 (42.76%), Postives = 261/442 (59.05%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVA--------SRVFLDFKGDYESMEGAAG 92
           +I+LAID  N G + YR+NN SYIF +  P VA         RV L F  +YES+E A+G
Sbjct: 82  TISLAIDKVNLGVLGYRSNNTSYIF-LDAPLVAFDIVFPKTCRVLLGFNSNYESIEKASG 141

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVL-------- 152
            TR++T LGL   + AISN F Y++  +PESFL+  QM+LE SKFKF+ R +        
Sbjct: 142 TTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLEGSKFKFIERSVIQSLKYGY 201

Query: 153 SICQGIVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
           +   G+ + S+ D        +Q+S +LQG+FG  + LYDSN+++I VDSI+Y IITTNI
Sbjct: 202 NFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDSNNKVIEVDSIYYAIITTNI 261

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           AF L+ C  + +   IRMPSDV       DSC V    R   IT + G C+D  N  + +
Sbjct: 262 AFQLHHC--NISTNFIRMPSDV------GDSCNV--QTRTATITGQNGFCVDASNGLHQD 321

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE---SSTTKWKVS 332
           DNSIIL  C  Q N++W F +  TI+ +   CLTF+    +++ NCSE    S  +W V+
Sbjct: 322 DNSIILYRCGYQSNQEWTFLSDKTIR-YSNKCLTFDTSRFVVLYNCSEVEKKSNIRWNVA 381

Query: 333 LDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQG---- 392
           +DGTI+NP SG VLT N ST+ ++L V ++     QGWRVGNYV+P+T SIIGL+     
Sbjct: 382 IDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTGSIIGLEEMCLE 441

Query: 393 -------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSMLSCMAGS 425
                        +K K  Q+ A      IRVN+ R+LCVSS ++    L+ +  C  G+
Sbjct: 442 ATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEEC-NGT 501

BLAST of Sed0017012 vs. ExPASy TrEMBL
Match: A0A1S3BDI7 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 7.5e-78
Identity = 189/442 (42.76%), Postives = 261/442 (59.05%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIFQVVCPTVA--------SRVFLDFKGDYESMEGAAG 92
           +I+LAID  N G + YR+NN SYIF +  P VA         RV L F  +YES+E A+G
Sbjct: 82  TISLAIDKVNLGVLGYRSNNTSYIF-LDAPLVAFDIVFPKTCRVLLGFNSNYESIEKASG 141

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVL-------- 152
            TR++T LGL   + AISN F Y++  +PESFL+  QM+LE SKFKF+ R +        
Sbjct: 142 TTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLEGSKFKFIERSVIQSLKYGY 201

Query: 153 SICQGIVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
           +   G+ + S+ D        +Q+S +LQG+FG  + LYDSN+++I VDSI+Y IITTNI
Sbjct: 202 NFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDSNNKVIEVDSIYYAIITTNI 261

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           AF L+ C  + +   IRMPSDV       DSC V    R   IT + G C+D  N  + +
Sbjct: 262 AFQLHHC--NISTNFIRMPSDV------GDSCNV--QTRTATITGQNGFCVDASNGLHQD 321

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE---SSTTKWKVS 332
           DNSIIL  C  Q N++W F +  TI+ +   CLTF+    +++ NCSE    S  +W V+
Sbjct: 322 DNSIILYRCGYQSNQEWTFLSDKTIR-YSNKCLTFDTSRFVVLYNCSEVEKKSNIRWNVA 381

Query: 333 LDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQG---- 392
           +DGTI+NP SG VLT N ST+ ++L V ++     QGWRVGNYV+P+T SIIGL+     
Sbjct: 382 IDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTGSIIGLEEMCLE 441

Query: 393 -------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSMLSCMAGS 425
                        +K K  Q+ A      IRVN+ R+LCVSS ++    L+ +  C  G+
Sbjct: 442 ATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEEC-NGT 501

BLAST of Sed0017012 vs. ExPASy TrEMBL
Match: A0A1S3C7Q1 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103497644 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 7.8e-75
Identity = 187/442 (42.31%), Postives = 256/442 (57.92%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYI--------FQVVCPTVASRVFLDFKGDYESMEGAAG 92
           +ITLA+D  N   + Y +NN SY+        FQVV P+   RV +DF  DY+S+E AA 
Sbjct: 80  TITLALDKVNLEVVGYLSNNTSYMFLDAPIEAFQVVFPSTC-RVIIDFYSDYKSIEIAAN 139

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFM--SRVLSICQG- 152
            TR ET LG    D AIS  F Y Q+ +P +FL+  QM++E  KFKF+  S  ++I  G 
Sbjct: 140 TTRFETLLGFDPIDVAISTLFHYRQDSVPNAFLVILQMVIEGMKFKFIEQSVFMNIKYGY 199

Query: 153 -----IVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
                +   S+ D        +Q S +LQG+FG ++ LYDSND+II VD+I YPII  NI
Sbjct: 200 NFKPSLAFVSLQDNWVKLSSQIQVSPSLQGIFGEVIQLYDSNDKIIQVDAIDYPIIIPNI 259

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           A  L+    + +  II+M          +D C V    R T I  R GLC+D++N+   +
Sbjct: 260 AMQLHH-HCNVSKKIIKM-------TVQNDPCNV--QSRTTHIIGRAGLCVDVENRLSND 319

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCS--ESSTTKWKVSL 332
            + +IL PC++Q+N++W FQ  GTIQ+ LG CLTFN  N ++I +CS  E S  KWKVS+
Sbjct: 320 GSPLILYPCRQQMNQRWTFQRDGTIQS-LGKCLTFNDTNFVLIYDCSKVEPSVIKWKVSV 379

Query: 333 DGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQGI---- 392
           DGTI+NP SG VLTAN+    TKL +  + +  GQ WRVGNY+ PI  SIIGL G+    
Sbjct: 380 DGTISNPSSGLVLTANSPARTTKLKMEANQQTIGQCWRVGNYINPIVGSIIGLDGVCLVA 439

Query: 393 -------------KIKVGQFMA------IRVNNDRSLCVS-SPTDGSAGLVSMLSCMAGS 425
                        K K  Q+ A      IRVN+ R+LCVS + +D + G +  +    GS
Sbjct: 440 TNNNTNIELDDCAKNKSEQYWALYGDGTIRVNSSRNLCVSLTSSDCTPGRIITVLMCNGS 499

BLAST of Sed0017012 vs. ExPASy TrEMBL
Match: A0A0A0K979 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 3.0e-74
Identity = 185/438 (42.24%), Postives = 251/438 (57.31%), Query Frame = 0

Query: 33  SITLAIDVTNAGFIAYRANNISYIF--------QVVCPTVASRVFLDFKGDYESMEGAAG 92
           +I+LAID  N G + YR+NN SYIF         VV P    RV L F  DYES+E A+G
Sbjct: 83  TISLAIDRVNLGVVGYRSNNNSYIFLDAPKAALDVVFPETC-RVVLGFNSDYESIEKASG 142

Query: 93  ITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFMSRVL-------- 152
            TR++T LGL   + AISN F Y +  +P SFL+  QM+LE SKFKF+ + +        
Sbjct: 143 TTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEGSKFKFIEQSVIQSLKYGY 202

Query: 153 SICQGIVVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHYPIITTNI 212
           +   G+ + S+ D        +Q+S +LQG+FG  + LYDSND+ I VDSI+Y IITTNI
Sbjct: 203 NFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSNDKFIDVDSIYYVIITTNI 262

Query: 213 AFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDIDNKDYFE 272
           AF L+ C  + +   IRMPSDV       DSC V    R   I+ + G C+D      + 
Sbjct: 263 AFQLHHC--NVSTNFIRMPSDV------DDSCNV--QTRTAMISGQNGFCVDSSRILDYN 322

Query: 273 DNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCS---ESSTTKWKVS 332
            N IIL  C  Q+N++W F +  TI+ +   CLTF     +++ NCS   E    +W V+
Sbjct: 323 GNPIILYRCINQLNQEWTFLSDKTIR-YSNKCLTFETSRYVVLYNCSEVEEKGNIRWNVA 382

Query: 333 LDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGLQG---- 392
           +DGTI+NP SG VLT + ST+R++L V ++     QGWRVGNYVKPI  SIIG++     
Sbjct: 383 IDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVKPIIGSIIGMEEMCLE 442

Query: 393 -------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSMLSCMAGS 421
                        +K K  Q+ A      IRVN  R+LCVSS ++ S  L+ +  C  G+
Sbjct: 443 ATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSGALLIIDEC-KGT 502

BLAST of Sed0017012 vs. ExPASy TrEMBL
Match: A0A6J1JQR5 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488061 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 8.1e-72
Identity = 179/448 (39.96%), Postives = 255/448 (56.92%), Query Frame = 0

Query: 25  NSFADDYRSITLAIDVTNAGFIAYRANNISYIF----QVVCPTV---ASRVFLDFKGDYE 84
           N + +D   I +A+DV     + YR+N+ SY+F    +V   TV     RV L F  D+E
Sbjct: 77  NPYGED---IFVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFETVFPSTCRVLLGFNSDFE 136

Query: 85  SMEGAAGITRMETFLGLGAFDRAISNWFDYNQEYLPESFLLFTQMLLEPSKFKFM--SRV 144
           S+E AA  +R++T LGL  F+ A+S+ F Y Q+      L   +  LE +KFKF+  S V
Sbjct: 137 SIENAAATSRLDTLLGLAPFESAVSDLFHYRQDLSLSRSLSSFKFFLEATKFKFIEQSVV 196

Query: 145 LSICQGI------VVSSILD--------LQSSVTLQGVFGTIVDLYDSNDEIILVDSIHY 204
            S+  G+       + S+ D        +Q+S +LQG+F   ++LYDSN  II VDSI+Y
Sbjct: 197 NSLKNGVPFKPTLAILSLQDNWAKLSXQIQASSSLQGLFREPIELYDSNHRIIQVDSIYY 256

Query: 205 PIITTNIAFMLYRCKDDATNTIIRMPSDVSDSDSDSDSCYVGRLRRRTRITRREGLCMDI 264
           PII  N+A  LY+C  +     IRMPS         D CYV    R  RI+  EG C++ 
Sbjct: 257 PIIIPNMALQLYQC--NIGTNFIRMPS------VGGDPCYV--QTRTARISGLEGFCVEA 316

Query: 265 DNKDYFEDNSIILNPCKEQVNKKWRFQNAGTIQAFLGTCLTFNLENSIIILNCSE--SST 324
           DNK + + N +IL+PC EQ+ ++W FQ+ G I  + G CL+FNL + ++  NCSE  + +
Sbjct: 317 DNKGWSDGNPVILSPCGEQLTQRWSFQSDGRI-GYSGKCLSFNLSSYVVTYNCSEGAAGS 376

Query: 325 TKWKVSLDGTITNPESGFVLTANASTSRTKLSVAIDSKAFGQGWRVGNYVKPITTSIIGL 384
            +WKVS++G I+NP S  VLTANAS     L+   ++K  GQ WRVGNYV PI  SIIGL
Sbjct: 377 IRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVGSIIGL 436

Query: 385 QG-----------------IKIKVGQFMA------IRVNNDRSLCVSSPTDGSAGLVSML 425
           +                  ++ K  Q+ A      IRVN+ R+ CVS+ +D   GL+++ 
Sbjct: 437 EETCLESMDNNAKVWLEKCVEQKAEQYWALYSDGSIRVNSSRNFCVSAASDSPGGLITIT 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884101.12.1e-8242.52seed lectin-like [Benincasa hispida][more]
XP_038877566.12.8e-7942.36seed lectin-like [Benincasa hispida][more]
XP_008445788.11.6e-7742.76PREDICTED: nigrin b-like [Cucumis melo] >KAA0033146.1 nigrin b-like [Cucumis mel... [more]
XP_023547577.13.8e-7640.40seed lectin-like [Cucurbita pepo subsp. pepo][more]
KAG6575323.15.0e-7640.63hypothetical protein SDJN03_25962, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
U3KRF84.9e-5835.06Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2[more]
P331831.2e-4532.04Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
P935431.3e-4231.57Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1[more]
Q413581.1e-4131.47Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
O224151.4e-4131.21Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A5D3D0Q47.5e-7842.76rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G... [more]
A0A1S3BDI77.5e-7842.76rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1[more]
A0A1S3C7Q17.8e-7542.31rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103497644 PE=3 SV=1[more]
A0A0A0K9793.0e-7442.24rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1[more]
A0A6J1JQR58.1e-7239.96rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488061 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 33..48
score: 45.72
coord: 116..136
score: 24.56
coord: 87..101
score: 37.19
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 226..346
e-value: 3.5E-14
score: 63.1
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 230..343
e-value: 6.0E-14
score: 52.4
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 230..346
score: 21.127501
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 371..424
score: 11.7945
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 236..343
e-value: 6.36029E-11
score: 57.9006
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 371..424
e-value: 2.59491E-6
score: 44.4187
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 29..135
e-value: 2.3E-7
score: 30.6
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 30..304
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 7..127
e-value: 2.2E-13
score: 52.3
NoneNo IPR availableGENE3D2.80.10.50coord: 222..350
e-value: 2.2E-22
score: 81.5
NoneNo IPR availableGENE3D2.80.10.50coord: 369..446
e-value: 6.6E-10
score: 40.7
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 30..304
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 148..198
e-value: 5.4E-7
score: 31.4
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 30..195
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 228..346
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 372..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0017012.1Sed0017012.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity