Sed0017008 (gene) Chayote v1

BLAST of Sed0017008 vs. NCBI nr
Match: XP_023543659.1 (uncharacterized protein LOC111803473 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1552.7 bits (4019), Expect = 0.0e+00
Identity = 816/1038 (78.61%), Postives = 882/1038 (84.97%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVS 240
            NFKSW PN S+ ANSSH VI D VKQ HQLVPNLIGGEFLDS +CPVV+V NPATQEVVS
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLVPNLIGGEFLDSHNCPVVDVINPATQEVVS 240

Query: 241  HVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGK 300
            HVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ +MF+FQ+LI+RDMDKLV NIV+EQGK
Sbjct: 241  HVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCIMFEFQKLILRDMDKLVTNIVTEQGK 300

Query: 301  PLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHSA 360
             LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH A
Sbjct: 301  TLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHPA 360

Query: 361  TISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFIC 420
            T+SLWMFPIAVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTHD+IN+IC
Sbjct: 361  TVSLWMFPIAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHDIINYIC 420

Query: 421  DDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDAG 480
            DDEDIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDAG
Sbjct: 421  DDEDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDAG 480

Query: 481  FGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLCR 540
            FGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  CR
Sbjct: 481  FGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFCR 540

Query: 541  LVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQA 600
            LV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM QA
Sbjct: 541  LVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-QA 600

Query: 601  DNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKAG 660
            DNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKAG
Sbjct: 601  DNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKAG 660

Query: 661  LEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSLH 720
            LEFYT+LKRVAQQWKN   IGVSM   SPSERHLR+R  PS L+STSEKDSPGG+HRSL 
Sbjct: 661  LEFYTKLKRVAQQWKNSAGIGVSMTASSPSERHLRSRTVPSTLVSTSEKDSPGGRHRSLP 720

Query: 721  PLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGDI 780
            PLPST      SDAVLL +P+ISQTDL NERAT+SPPTP+RDLHG+GLSLIS LSS GDI
Sbjct: 721  PLPSTSERDSPSDAVLLPDPRISQTDLANERATSSPPTPDRDLHGQGLSLISTLSSEGDI 780

Query: 781  SNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHVP 840
            SN DLSPAM  TGDR+L  QAM      SSER+Y+ QK HWSE  RA+S PS+SER    
Sbjct: 781  SNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMYLSQKCHWSESLRAESIPSSSER---- 840

Query: 841  SSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSER 900
                  I  Q SRTT PA VL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSER
Sbjct: 841  ------IKGQASRTT-PAFVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSER 900

Query: 901  GHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQLS 960
             +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       +
Sbjct: 901  VYMLATSHLNDSMSQTLRRTDTPLFSSSDGKDHISLDSQTDAALQSDRMYLSSLSERDGN 960

Query: 961  LASHIDISSQ------LTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVS 1002
            +AS    SSQ       TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVS
Sbjct: 961  MAS---ASSQQVESLTSTSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVS 1020

BLAST of Sed0017008 vs. NCBI nr
Match: XP_022978818.1 (uncharacterized protein LOC111478665 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1550.4 bits (4013), Expect = 0.0e+00
Identity = 811/1032 (78.59%), Postives = 878/1032 (85.08%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVS 240
            NFKSW PN S+ ANSSH VI D VKQ HQLVPNLIGGEFLDS +CPVV+V NPATQEVVS
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLVPNLIGGEFLDSHNCPVVDVINPATQEVVS 240

Query: 241  HVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGK 300
            HVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV+EQGK
Sbjct: 241  HVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVTEQGK 300

Query: 301  PLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHSA 360
             LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH A
Sbjct: 301  TLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHPA 360

Query: 361  TISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFIC 420
            T+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTH++IN+IC
Sbjct: 361  TVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHEIINYIC 420

Query: 421  DDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDAG 480
            DD DIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDAG
Sbjct: 421  DDGDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDAG 480

Query: 481  FGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLCR 540
            FGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  CR
Sbjct: 481  FGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFCR 540

Query: 541  LVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQA 600
            LV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM QA
Sbjct: 541  LVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-QA 600

Query: 601  DNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKAG 660
            DNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKAG
Sbjct: 601  DNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKAG 660

Query: 661  LEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSLH 720
            LEFYT+LKRVAQQWKN   IGVSM   SPSE HLR R  PS L+STSEKDSPGG+HRSL 
Sbjct: 661  LEFYTKLKRVAQQWKNSAGIGVSMTASSPSESHLRPRTVPSTLVSTSEKDSPGGRHRSLP 720

Query: 721  PLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGDI 780
            PL ST      SDAVLL +P++SQTDL NERAT+SPP+P+RDLHG+GLSLIS LSS GDI
Sbjct: 721  PLSSTSERDSPSDAVLLPDPRLSQTDLANERATSSPPSPDRDLHGQGLSLISTLSSEGDI 780

Query: 781  SNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHVP 840
            SN DLSPAM  TGDR+L  QAM      SSER++I QK HWSE  RADS PS+SER    
Sbjct: 781  SNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMHISQKCHWSESLRADSIPSSSER---- 840

Query: 841  SSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSER 900
                  I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSER
Sbjct: 841  ------IKGQASRTTPPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSER 900

Query: 901  GHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQLS 960
             +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       +
Sbjct: 901  VYMLATSHLNDSMSQTLRRTDTPLFSSSNGKDHISLDSQTDAALQSDRMYLSSLSERDGN 960

Query: 961  LASHIDISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQG 1002
            +AS   ++S  TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVSADE Q 
Sbjct: 961  MASASSLTS--TSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVSADESQD 1019

BLAST of Sed0017008 vs. NCBI nr
Match: XP_023543654.1 (uncharacterized protein LOC111803473 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543655.1 uncharacterized protein LOC111803473 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543656.1 uncharacterized protein LOC111803473 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543657.1 uncharacterized protein LOC111803473 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1548.1 bits (4007), Expect = 0.0e+00
Identity = 816/1039 (78.54%), Postives = 882/1039 (84.89%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQL-VPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN S+ ANSSH VI D VKQ HQL VPNLIGGEFLDS +CPVV+V NPATQEVV
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLKVPNLIGGEFLDSHNCPVVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            SHVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ +MF+FQ+LI+RDMDKLV NIV+EQG
Sbjct: 241  SHVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCIMFEFQKLILRDMDKLVTNIVTEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH 
Sbjct: 301  KTLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            AT+SLWMFPIAVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTHD+IN+I
Sbjct: 361  ATVSLWMFPIAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHDIINYI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDDEDIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDA
Sbjct: 421  CDDEDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  C
Sbjct: 481  GFGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM Q
Sbjct: 541  RLVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            ADNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKA
Sbjct: 601  ADNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKA 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
            GLEFYT+LKRVAQQWKN   IGVSM   SPSERHLR+R  PS L+STSEKDSPGG+HRSL
Sbjct: 661  GLEFYTKLKRVAQQWKNSAGIGVSMTASSPSERHLRSRTVPSTLVSTSEKDSPGGRHRSL 720

Query: 721  HPLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGD 780
             PLPST      SDAVLL +P+ISQTDL NERAT+SPPTP+RDLHG+GLSLIS LSS GD
Sbjct: 721  PPLPSTSERDSPSDAVLLPDPRISQTDLANERATSSPPTPDRDLHGQGLSLISTLSSEGD 780

Query: 781  ISNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHV 840
            ISN DLSPAM  TGDR+L  QAM      SSER+Y+ QK HWSE  RA+S PS+SER   
Sbjct: 781  ISNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMYLSQKCHWSESLRAESIPSSSER--- 840

Query: 841  PSSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSE 900
                   I  Q SRTT PA VL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSE
Sbjct: 841  -------IKGQASRTT-PAFVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSE 900

Query: 901  RGHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQL 960
            R +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       
Sbjct: 901  RVYMLATSHLNDSMSQTLRRTDTPLFSSSDGKDHISLDSQTDAALQSDRMYLSSLSERDG 960

Query: 961  SLASHIDISSQ------LTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIV 1002
            ++AS    SSQ       TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IV
Sbjct: 961  NMAS---ASSQQVESLTSTSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIV 1020

BLAST of Sed0017008 vs. NCBI nr
Match: XP_038880953.1 (uncharacterized protein LOC120072618 isoform X2 [Benincasa hispida])

HSP 1 Score: 1547.7 bits (4006), Expect = 0.0e+00
Identity = 804/1025 (78.44%), Postives = 876/1025 (85.46%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+Q Q  L+ QKKM  PQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQGQTGLVAQKKMHPPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGGVYRNRRKIDES RKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL +MSEH
Sbjct: 61   RGGVYRNRRKIDESPRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKNMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEV+QIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVKQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEN 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVS 240
            NFKSW PN SVP N SH VI D+V+Q HQLVPNL+GG+FLDS +CPVV+V NPATQEVVS
Sbjct: 181  NFKSWRPNISVPTNGSHTVIGDSVQQNHQLVPNLVGGKFLDSHNCPVVDVINPATQEVVS 240

Query: 241  HVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGK 300
            HVPLTTYEEFKAAVNAAK+AFPSW+NTPISTRQ VMFKFQELI+RDMDKLV NIV+EQGK
Sbjct: 241  HVPLTTYEEFKAAVNAAKQAFPSWRNTPISTRQCVMFKFQELILRDMDKLVTNIVAEQGK 300

Query: 301  PLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHSA 360
             L+DAQDDIICGLEVVK+ACGLA MQMGEF+ NASDGID +CIREPIGVCAGICS NH A
Sbjct: 301  TLRDAQDDIICGLEVVKHACGLAAMQMGEFVPNASDGIDSYCIREPIGVCAGICSLNHPA 360

Query: 361  TISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFIC 420
            T+SLWMFPIAVTCGNTFVLKPCE +PGAS+LLAALAVEAGLPDGVLNIVHGTHD+I++IC
Sbjct: 361  TVSLWMFPIAVTCGNTFVLKPCETHPGASMLLAALAVEAGLPDGVLNIVHGTHDIIDYIC 420

Query: 421  DDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDAG 480
            DDEDIKAIS +SSSS  KNIYARAA TGK+VQS+ GGKSHAIIMPDANMEA L+ALV+AG
Sbjct: 421  DDEDIKAISFSSSSSVGKNIYARAAVTGKQVQSHFGGKSHAIIMPDANMEATLSALVEAG 480

Query: 481  FGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLCR 540
                 +TCMA  I++SVGSS++WEEKLVE AKALKVNVGTDP A LGPVITKEVK+  C+
Sbjct: 481  -----RTCMATVIIVSVGSSIMWEEKLVECAKALKVNVGTDPKADLGPVITKEVKNRFCK 540

Query: 541  LVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQA 600
            LV+SGI+ GARLLLDGRDIVVP YENGNFVGPTILSDVTTDMECYKEEFFGPVLLCM QA
Sbjct: 541  LVQSGIEDGARLLLDGRDIVVPEYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCM-QA 600

Query: 601  DNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKAG 660
            DNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKAG
Sbjct: 601  DNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKAG 660

Query: 661  LEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSLH 720
            LEFYTQLKRVAQQWKN P IGVSMA+PSPS+RHLR+RA PSML+STS+KDSPG + RSL 
Sbjct: 661  LEFYTQLKRVAQQWKNSPSIGVSMAVPSPSKRHLRSRAVPSMLVSTSDKDSPGMRQRSLP 720

Query: 721  PLPSTSD------AVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGDI 780
            PLPSTS+      AVLL N QISQT L NERAT+SPPT +RDLHG+GLSLIS LSS GD+
Sbjct: 721  PLPSTSERDSPSVAVLLPNSQISQTGLANERATSSPPTSDRDLHGQGLSLISTLSSEGDV 780

Query: 781  SNHDLSPAMPSTGDRDLASQA------MSSERLYIPQKSHWSEVQRADSFPSTSERTHVP 840
            SN DLSPAM S G RDL  QA       SSERLY+PQKSHW+   RADS PS SER H P
Sbjct: 781  SNQDLSPAMLSAGGRDLPGQATSVATSRSSERLYVPQKSHWNGTLRADSIPSNSERIHAP 840

Query: 841  SSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSER 900
            SS TNSI  Q SRT+HP  VL  E LY PTS  SIC I  GND+T+PSRRIDS C SSER
Sbjct: 841  SSHTNSIKGQASRTSHPGSVLATEGLYLPTSHGSICLIDHGNDNTIPSRRIDSMCRSSER 900

Query: 901  GHMLATSHLNDSMNQTLQATETSLF---------SSSARQYMAPAHR---SQLSLASHID 960
             +MLATSHLND+M+QTLQ T+TSL          S+S R Y++       +  S AS   
Sbjct: 901  VYMLATSHLNDTMSQTLQQTDTSLLPSHTDVALQSTSNRMYLSSLSERDDNMSSTASQQA 960

Query: 961  ISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQGQEASLT 1001
             S   TS+R+YI PL HRN+ M  KSEWLCIPT AV+QRMY Q  +V ADEFQ QEASLT
Sbjct: 961  ESLTSTSERMYIPPLVHRNQGMAPKSEWLCIPTPAVSQRMYPQGPVVPADEFQSQEASLT 1019

BLAST of Sed0017008 vs. NCBI nr
Match: XP_022978814.1 (uncharacterized protein LOC111478665 isoform X1 [Cucurbita maxima] >XP_022978815.1 uncharacterized protein LOC111478665 isoform X1 [Cucurbita maxima] >XP_022978816.1 uncharacterized protein LOC111478665 isoform X1 [Cucurbita maxima] >XP_022978817.1 uncharacterized protein LOC111478665 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1545.8 bits (4001), Expect = 0.0e+00
Identity = 811/1033 (78.51%), Postives = 878/1033 (85.00%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQL-VPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN S+ ANSSH VI D VKQ HQL VPNLIGGEFLDS +CPVV+V NPATQEVV
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLMVPNLIGGEFLDSHNCPVVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            SHVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV+EQG
Sbjct: 241  SHVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVTEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH 
Sbjct: 301  KTLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            AT+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTH++IN+I
Sbjct: 361  ATVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHEIINYI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDD DIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDA
Sbjct: 421  CDDGDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  C
Sbjct: 481  GFGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM Q
Sbjct: 541  RLVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            ADNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKA
Sbjct: 601  ADNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKA 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
            GLEFYT+LKRVAQQWKN   IGVSM   SPSE HLR R  PS L+STSEKDSPGG+HRSL
Sbjct: 661  GLEFYTKLKRVAQQWKNSAGIGVSMTASSPSESHLRPRTVPSTLVSTSEKDSPGGRHRSL 720

Query: 721  HPLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGD 780
             PL ST      SDAVLL +P++SQTDL NERAT+SPP+P+RDLHG+GLSLIS LSS GD
Sbjct: 721  PPLSSTSERDSPSDAVLLPDPRLSQTDLANERATSSPPSPDRDLHGQGLSLISTLSSEGD 780

Query: 781  ISNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHV 840
            ISN DLSPAM  TGDR+L  QAM      SSER++I QK HWSE  RADS PS+SER   
Sbjct: 781  ISNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMHISQKCHWSESLRADSIPSSSER--- 840

Query: 841  PSSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSE 900
                   I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSE
Sbjct: 841  -------IKGQASRTTPPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSE 900

Query: 901  RGHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQL 960
            R +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       
Sbjct: 901  RVYMLATSHLNDSMSQTLRRTDTPLFSSSNGKDHISLDSQTDAALQSDRMYLSSLSERDG 960

Query: 961  SLASHIDISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQ 1002
            ++AS   ++S  TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVSADE Q
Sbjct: 961  NMASASSLTS--TSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVSADESQ 1020

BLAST of Sed0017008 vs. ExPASy Swiss-Prot
Match: Q0WM29 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALDH6B2 PE=1 SV=2)

HSP 1 Score: 650.6 bits (1677), Expect = 2.9e-185
Identity = 313/496 (63.10%), Postives = 400/496 (80.65%), Query Frame = 0

Query: 210 VPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPIS 269
           VPNLIGG F++S+    ++V NPATQEVVS VPLTT EEFKAAV+AAK+AFP W+NTPI+
Sbjct: 114 VPNLIGGSFVESQSSSFIDVINPATQEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPIT 173

Query: 270 TRQHVMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEF 329
           TRQ VM KFQELI ++MDKL MNI +EQGK LKD+  DI  GLEVV++ACG+AT+QMGE+
Sbjct: 174 TRQRVMLKFQELIRKNMDKLAMNITTEQGKTLKDSHGDIFRGLEVVEHACGMATLQMGEY 233

Query: 330 IRNASDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASL 389
           + N S+G+D + IREP+GVCAGIC FN  A I LWMFP+AVTCGNTF+LKP EK+PGAS+
Sbjct: 234 LPNVSNGVDTYSIREPLGVCAGICPFNFPAMIPLWMFPVAVTCGNTFILKPSEKDPGASV 293

Query: 390 LLAALAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKK 449
           +LA LA+EAGLPDGVLNIVHGT+D +N ICDDEDI+A+S   S++A  +IYARAAA GK+
Sbjct: 294 ILAELAMEAGLPDGVLNIVHGTNDTVNAICDDEDIRAVSFVGSNTAGMHIYARAAAKGKR 353

Query: 450 VQSNLGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEY 509
           +QSN+G K+H +++PDAN++A LNAL+ AGFG A Q CMA++ V+ VG +  WE+KLVE 
Sbjct: 354 IQSNMGAKNHGLVLPDANIDATLNALLAAGFGAAGQRCMALSTVVFVGDAKSWEDKLVER 413

Query: 510 AKALKVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFV 569
           AKALKV  G++P A LGPVI+K+ K+ +CRL++SG+D GA+LLLDGRDIVVPGYE GNF+
Sbjct: 414 AKALKVTCGSEPDADLGPVISKQAKERICRLIQSGVDDGAKLLLDGRDIVVPGYEKGNFI 473

Query: 570 GPTILSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYAR 629
           GPTILS VT DMECYKEE FGPVL+CM QA++ +EAI+I+N+NK  NGA+IFT+SG  AR
Sbjct: 474 GPTILSGVTPDMECYKEEIFGPVLVCM-QANSFDEAISIINKNKYGNGAAIFTSSGAAAR 533

Query: 630 KFQSEVEVGMVGINVAVPVPLP--------------SSFNDKAGLEFYTQLKRVAQQWKN 689
           KFQ ++E G +GINV +PVPLP               +F  KAG++F+TQ+K V QQWK+
Sbjct: 534 KFQMDIEAGQIGINVPIPVPLPFFSFTGNKASFAGDLNFYGKAGVDFFTQIKTVTQQWKD 593

Query: 690 LPDIGVSMAIPSPSER 692
           +P   VS+A+P+  ++
Sbjct: 594 IP-TSVSLAMPTSQKQ 607

BLAST of Sed0017008 vs. ExPASy Swiss-Prot
Match: P52713 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Caenorhabditis elegans OX=6239 GN=alh-8 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.3e-136
Identity = 250/488 (51.23%), Postives = 326/488 (66.80%), Query Frame = 0

Query: 214 IGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQH 273
           I G+ ++S+    VE+TNPAT EV++ VP  T  E +AAV++AK AF +WKNT   TRQ 
Sbjct: 34  IDGQAVESKTTDFVELTNPATNEVIAMVPNATQAEMQAAVDSAKNAFNTWKNTSPLTRQQ 93

Query: 274 VMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNA 333
            MFK Q LI RDM KL  +I  EQGK L DA+ D+  GL+VV++AC + ++ MGE + N 
Sbjct: 94  CMFKLQALIKRDMKKLAESITIEQGKTLPDAEGDVSRGLQVVEHACSVPSLMMGETLPNV 153

Query: 334 SDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAA 393
           S  +D H  R P+GV AGIC FN  A I LWMFP+A+  GNT V+KP E++PGA+ LL  
Sbjct: 154 SRDMDTHSYRIPLGVTAGICPFNFPAMIPLWMFPVALATGNTMVIKPSEQDPGAAQLLVE 213

Query: 394 LAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKKVQSN 453
           LA EAG+PDG +NI+HG H  +NFICD+ DIKAIS     +A K+IY R A  GK+VQSN
Sbjct: 214 LAKEAGVPDGCVNIIHGQHSAVNFICDNPDIKAISFVGGDAAGKHIYERGAKNGKRVQSN 273

Query: 454 LGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEYAKAL 513
           +G K+H +IM DAN E  LN L  A FG A Q CMA+   + VG +  W  +LVE AK L
Sbjct: 274 MGAKNHGVIMADANKEQTLNQLTAAAFGAAGQRCMALTTAVLVGEARAWLPELVEKAKNL 333

Query: 514 KVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTI 573
           KVN G  P   +GP+I+K+ K  + RL+ S    GA++ LDG +I VPG+ENGNFVGPTI
Sbjct: 334 KVNAGWKPDTDIGPLISKQSKARVLRLIESAKKEGAQVPLDGSNITVPGFENGNFVGPTI 393

Query: 574 LSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQS 633
           L+ V  +M CY+EE FGPVL+ M +A+NL EAI I+N N   NG +IFT++G  ARKF +
Sbjct: 394 LAGVKPNMTCYREEIFGPVLVVM-EAENLNEAIEIINNNPYGNGTAIFTSNGATARKFTN 453

Query: 634 EVEVGMVGINVAVPVPLPS--------------SFNDKAGLEFYTQLKRVAQQW-KNLPD 687
           EV+VG +GINV +PVPLP               +F  KAG++FYTQ K V Q W ++L +
Sbjct: 454 EVDVGQIGINVPIPVPLPMFSFTGSRGSFLGDLNFYGKAGIQFYTQWKTVTQYWNESLTE 513

BLAST of Sed0017008 vs. ExPASy Swiss-Prot
Match: Q02252 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Homo sapiens OX=9606 GN=ALDH6A1 PE=1 SV=2)

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-134
Identity = 236/490 (48.16%), Postives = 328/490 (66.94%), Query Frame = 0

Query: 214 IGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQH 273
           IGG+F++S+    +++ NPAT EV+  VP  T  E  AA+ + KRAFP+W +T + +RQ 
Sbjct: 44  IGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAFPAWADTSVLSRQQ 103

Query: 274 VMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNA 333
           V+ ++Q+LI  ++ ++   I  EQGK L DA+ D+  GL+VV++AC + ++ MGE + + 
Sbjct: 104 VLLRYQQLIKENLKEIAKLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMMGETMPSI 163

Query: 334 SDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAA 393
           +  +D +  R P+GVCAGI  FN  A I LWMFP+A+ CGNTF++KP E+ PGA++LLA 
Sbjct: 164 TKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAK 223

Query: 394 LAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKKVQSN 453
           L  ++G PDG LNI+HG H+ +NFICD  DIKAIS   S+ A + I+ R +  GK+VQ+N
Sbjct: 224 LLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEYIFERGSRHGKRVQAN 283

Query: 454 LGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEYAKAL 513
           +G K+H ++MPDAN E  LN LV A FG A Q CMA++  + VG +  W  +LVE+AK L
Sbjct: 284 MGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNL 343

Query: 514 KVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTI 573
           +VN G  P A LGP+IT + K+ +C L+ SG   GA +LLDGR I V GYENGNFVGPTI
Sbjct: 344 RVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTI 403

Query: 574 LSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQS 633
           +S+V  +M CYKEE FGPVL+ + + + L+EAI IVN N   NG +IFTT+G  ARK+  
Sbjct: 404 ISNVKPNMTCYKEEIFGPVLVVL-ETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAH 463

Query: 634 EVEVGMVGINVAVPVPLP--------------SSFNDKAGLEFYTQLKRVAQQWKNLPDI 690
            V+VG VG+NV +PVPLP              ++F  K G++FYTQLK +  QWK     
Sbjct: 464 LVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDAT 523

BLAST of Sed0017008 vs. ExPASy Swiss-Prot
Match: Q07536 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Bos taurus OX=9913 GN=ALDH6A1 PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.8e-134
Identity = 244/521 (46.83%), Postives = 341/521 (65.45%), Query Frame = 0

Query: 168 LKAKIRQGNLSETNFKSWSPNSSVPANSSHVIEDAVKQKHQLVPNLIGGEFLDSRDCPVV 227
           L+A+I Q  +S     SW P SS  ++S   ++             I G+F++S+    +
Sbjct: 13  LRARILQ--VSSKVNSSWQPASSFSSSSVPTVK-----------LFIDGKFIESKSDKWI 72

Query: 228 EVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMD 287
           ++ NPAT EV+  VP +T  E  AAV++ KR FP+W +T I +RQ V+ ++Q+LI  ++ 
Sbjct: 73  DIHNPATNEVIGRVPESTKAEMDAAVSSCKRTFPAWADTSILSRQQVLLRYQQLIKENLK 132

Query: 288 KLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIG 347
           ++   I+ EQGK L DA+ D+  GL+VV++AC + ++ +G+ + + +  +D +  R P+G
Sbjct: 133 EIARLIMLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGDTMPSITKDMDLYSYRLPLG 192

Query: 348 VCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNI 407
           VCAGI  FN  A I LWMFP+A+ CGNTF++KP E+ PGA++LLA L  ++G PDG LNI
Sbjct: 193 VCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGATMLLAKLFQDSGAPDGTLNI 252

Query: 408 VHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDAN 467
           +HG H+ +NFICD  DIKAIS   S+ A + I+ R +  GK+VQ+N+G K+H ++MPDAN
Sbjct: 253 IHGQHEAVNFICDHPDIKAISFVGSNQAGEYIFERGSRHGKRVQANMGAKNHGVVMPDAN 312

Query: 468 MEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGP 527
            E  LN LV A FG A Q CMA++  I VG +  W  +LVE AK L+VN G  P A LGP
Sbjct: 313 KENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVERAKKLRVNAGDQPGADLGP 372

Query: 528 VITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEE 587
           +IT + K+ +C L+ SG   GA +LLDGR I V GYENGNFVGPTI+S+V  +M CYKEE
Sbjct: 373 LITPQAKERVCNLIDSGTKEGASILLDGRSIKVKGYENGNFVGPTIISNVKPNMTCYKEE 432

Query: 588 FFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVP 647
            FGPVL+ + + D L+EAI IVN N   NG +IFTT+G  ARK+   V+VG VG+NV +P
Sbjct: 433 IFGPVLVVL-ETDTLDEAIKIVNDNPYGNGTAIFTTNGATARKYSHLVDVGQVGVNVPIP 492

Query: 648 VPLP--------------SSFNDKAGLEFYTQLKRVAQQWK 675
           VPLP              ++F  K G++FYTQLK +  QWK
Sbjct: 493 VPLPMFSFTGSRASFRGDTNFYGKQGIQFYTQLKTITSQWK 519

BLAST of Sed0017008 vs. ExPASy Swiss-Prot
Match: Q02253 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Rattus norvegicus OX=10116 GN=Aldh6a1 PE=1 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.1e-134
Identity = 243/520 (46.73%), Postives = 337/520 (64.81%), Query Frame = 0

Query: 184 SWSPNSSVPANSSHVIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPL 243
           +W P SS  ++S   ++             I G+F++S+    +++ NPAT EVV  VP 
Sbjct: 25  TWYPASSFSSSSVPTVK-----------LFIDGKFVESKSDKWIDIHNPATNEVVGRVPQ 84

Query: 244 TTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGKPLKD 303
           +T  E +AAV A KRAFP+W +T I +RQ V+ ++Q+LI  ++ ++   I  EQGK L D
Sbjct: 85  STKAEMEAAVAACKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLAD 144

Query: 304 AQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHSATISL 363
           A+ D+  GL+VV++AC + ++ +GE + + +  +D +  R P+GVCAGI  FN  A I L
Sbjct: 145 AEGDVFRGLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPL 204

Query: 364 WMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFICDDED 423
           WMFP+A+ CGNTF++KP E+ PGA++LLA L  ++G PDG LNI+HG H+ +NFICD  D
Sbjct: 205 WMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPD 264

Query: 424 IKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDAGFGTA 483
           IKAIS   S+ A + I+ R +  GK+VQ+N+G K+H ++MPDAN E  LN LV A FG A
Sbjct: 265 IKAISFVGSNQAGEYIFERGSRNGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAA 324

Query: 484 RQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLCRLVRS 543
            Q CMA++  + VG +  W  +LVE AK L+VN G  P A LGP+IT + K+ +C L+ S
Sbjct: 325 GQRCMALSTAVLVGEAKKWLPELVERAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDS 384

Query: 544 GIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQADNLE 603
           G   GA +LLDGR I V GYENGNFVGPTI+S+V   M CYKEE FGPVL+ + + + L+
Sbjct: 385 GAKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFGPVLVVL-ETETLD 444

Query: 604 EAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLP------------ 663
           EAI IVN N   NG +IFTT+G  ARK+   V+VG VG+NV +PVPLP            
Sbjct: 445 EAIKIVNDNPYGNGTAIFTTNGAIARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSSFR 504

Query: 664 --SSFNDKAGLEFYTQLKRVAQQWKNLPDIGVSMAIPSPS 690
             ++F  K G++FYTQLK +  QWK       S A+  P+
Sbjct: 505 GDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPT 532

BLAST of Sed0017008 vs. ExPASy TrEMBL
Match: A0A6J1IUC7 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita maxima OX=3661 GN=LOC111478665 PE=3 SV=1)

HSP 1 Score: 1550.4 bits (4013), Expect = 0.0e+00
Identity = 811/1032 (78.59%), Postives = 878/1032 (85.08%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVS 240
            NFKSW PN S+ ANSSH VI D VKQ HQLVPNLIGGEFLDS +CPVV+V NPATQEVVS
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLVPNLIGGEFLDSHNCPVVDVINPATQEVVS 240

Query: 241  HVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGK 300
            HVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV+EQGK
Sbjct: 241  HVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVTEQGK 300

Query: 301  PLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHSA 360
             LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH A
Sbjct: 301  TLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHPA 360

Query: 361  TISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFIC 420
            T+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTH++IN+IC
Sbjct: 361  TVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHEIINYIC 420

Query: 421  DDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDAG 480
            DD DIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDAG
Sbjct: 421  DDGDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDAG 480

Query: 481  FGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLCR 540
            FGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  CR
Sbjct: 481  FGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFCR 540

Query: 541  LVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQA 600
            LV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM QA
Sbjct: 541  LVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-QA 600

Query: 601  DNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKAG 660
            DNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKAG
Sbjct: 601  DNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKAG 660

Query: 661  LEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSLH 720
            LEFYT+LKRVAQQWKN   IGVSM   SPSE HLR R  PS L+STSEKDSPGG+HRSL 
Sbjct: 661  LEFYTKLKRVAQQWKNSAGIGVSMTASSPSESHLRPRTVPSTLVSTSEKDSPGGRHRSLP 720

Query: 721  PLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGDI 780
            PL ST      SDAVLL +P++SQTDL NERAT+SPP+P+RDLHG+GLSLIS LSS GDI
Sbjct: 721  PLSSTSERDSPSDAVLLPDPRLSQTDLANERATSSPPSPDRDLHGQGLSLISTLSSEGDI 780

Query: 781  SNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHVP 840
            SN DLSPAM  TGDR+L  QAM      SSER++I QK HWSE  RADS PS+SER    
Sbjct: 781  SNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMHISQKCHWSESLRADSIPSSSER---- 840

Query: 841  SSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSER 900
                  I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSER
Sbjct: 841  ------IKGQASRTTPPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSER 900

Query: 901  GHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQLS 960
             +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       +
Sbjct: 901  VYMLATSHLNDSMSQTLRRTDTPLFSSSNGKDHISLDSQTDAALQSDRMYLSSLSERDGN 960

Query: 961  LASHIDISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQG 1002
            +AS   ++S  TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVSADE Q 
Sbjct: 961  MASASSLTS--TSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVSADESQD 1019

BLAST of Sed0017008 vs. ExPASy TrEMBL
Match: A0A6J1IV39 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita maxima OX=3661 GN=LOC111478665 PE=3 SV=1)

HSP 1 Score: 1545.8 bits (4001), Expect = 0.0e+00
Identity = 811/1033 (78.51%), Postives = 878/1033 (85.00%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQL-VPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN S+ ANSSH VI D VKQ HQL VPNLIGGEFLDS +CPVV+V NPATQEVV
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLMVPNLIGGEFLDSHNCPVVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            SHVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV+EQG
Sbjct: 241  SHVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVTEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH 
Sbjct: 301  KTLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            AT+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTH++IN+I
Sbjct: 361  ATVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHEIINYI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDD DIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDA
Sbjct: 421  CDDGDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  C
Sbjct: 481  GFGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM Q
Sbjct: 541  RLVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            ADNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKA
Sbjct: 601  ADNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKA 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
            GLEFYT+LKRVAQQWKN   IGVSM   SPSE HLR R  PS L+STSEKDSPGG+HRSL
Sbjct: 661  GLEFYTKLKRVAQQWKNSAGIGVSMTASSPSESHLRPRTVPSTLVSTSEKDSPGGRHRSL 720

Query: 721  HPLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGD 780
             PL ST      SDAVLL +P++SQTDL NERAT+SPP+P+RDLHG+GLSLIS LSS GD
Sbjct: 721  PPLSSTSERDSPSDAVLLPDPRLSQTDLANERATSSPPSPDRDLHGQGLSLISTLSSEGD 780

Query: 781  ISNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHV 840
            ISN DLSPAM  TGDR+L  QAM      SSER++I QK HWSE  RADS PS+SER   
Sbjct: 781  ISNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMHISQKCHWSESLRADSIPSSSER--- 840

Query: 841  PSSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSE 900
                   I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSE
Sbjct: 841  -------IKGQASRTTPPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSE 900

Query: 901  RGHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQL 960
            R +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       
Sbjct: 901  RVYMLATSHLNDSMSQTLRRTDTPLFSSSNGKDHISLDSQTDAALQSDRMYLSSLSERDG 960

Query: 961  SLASHIDISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQ 1002
            ++AS   ++S  TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVSADE Q
Sbjct: 961  NMASASSLTS--TSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVSADESQ 1020

BLAST of Sed0017008 vs. ExPASy TrEMBL
Match: A0A6J1BWY1 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Momordica charantia OX=3673 GN=LOC111005478 PE=4 SV=1)

HSP 1 Score: 1540.8 bits (3988), Expect = 0.0e+00
Identity = 809/1042 (77.64%), Postives = 878/1042 (84.26%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  LI Q KML PQ GRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLIVQNKMLPPQSGRFQDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGGVYRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKN DHNHEPL DMSEH
Sbjct: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDVWMLTIKNEDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEV+QIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVKQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQ-LVPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN SVPANSS  VI +  KQ  Q  VPN IGGEFLDSR+C +V+V NPATQEVV
Sbjct: 181  NFKSWRPNISVPANSSDTVIGNTAKQNLQPKVPNFIGGEFLDSRNCSMVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            S VPLTTYEEFKAAVNAAK+AFPSWKNTPIS RQ +MFKFQELI+RDMDKLV NIV+EQG
Sbjct: 241  SQVPLTTYEEFKAAVNAAKQAFPSWKNTPISARQRIMFKFQELILRDMDKLVTNIVTEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LK AQDDI+CGLEVVK+ACGLA MQMG+F+ NASDGID +CIREP+GVCAGICS NH 
Sbjct: 301  KTLKGAQDDILCGLEVVKHACGLAAMQMGKFVPNASDGIDSYCIREPLGVCAGICSLNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            ATISLWMFPIAVTCGNTFVLKPCEK+PGAS+LLAALAVEAGLPDGVLNIVHGTHD+I+ I
Sbjct: 361  ATISLWMFPIAVTCGNTFVLKPCEKHPGASMLLAALAVEAGLPDGVLNIVHGTHDIIDSI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDDEDIK IS ASSS+A KNI+ARAAATGK+VQSNLGGKSHAIIMPDA+M A+ +A+V A
Sbjct: 421  CDDEDIKVISFASSSTAGKNIHARAAATGKQVQSNLGGKSHAIIMPDADMGASFSAVVGA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGTA QTCM +NIV+SVGSS+LWEEKLVE AKAL+VNVGTDP A LGPV+TKEVKD LC
Sbjct: 481  GFGTAGQTCMTLNIVVSVGSSILWEEKLVECAKALEVNVGTDPNADLGPVVTKEVKDRLC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+S I+ GARLLLDGR+IVVPGYENGNF GPTILSDVTTDMECYKEEFFGPVLLCM Q
Sbjct: 541  RLVQSSIEGGARLLLDGRNIVVPGYENGNFFGPTILSDVTTDMECYKEEFFGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            A+NLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAVPVPLPSSFNDKA
Sbjct: 601  AENLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
             LEFYT+LKRVAQQWKNLP IGVSMA PSPSER LRARA P +L S  EKDSPGG H SL
Sbjct: 661  DLEFYTKLKRVAQQWKNLPSIGVSMATPSPSERDLRARAVPPVLASMCEKDSPGG-HGSL 720

Query: 721  HPLPSTSDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGDISNHDL 780
             PLP         NP+ISQTDL  ERA +SPPTP+RDLHG GLSLIS LSS GD+SN DL
Sbjct: 721  PPLP---------NPRISQTDLTIERAASSPPTPDRDLHGPGLSLISTLSSEGDVSNQDL 780

Query: 781  SPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHVPSSLTN 840
            SPAMPS  D +L  QAM      SSERLYIPQKSHW+E QRADS P +SE+ H PSS TN
Sbjct: 781  SPAMPSAADSNLPGQAMSMATSRSSERLYIPQKSHWNETQRADSIPPSSEQIHGPSSHTN 840

Query: 841  SIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSERGHMLA 900
            SI AQ SR+THPALVL AE LY PTS DSIC I  G+DSTVPSRRI S C  SER +MLA
Sbjct: 841  SIKAQASRSTHPALVLAAEGLYVPTSHDSICLITHGSDSTVPSRRIGSICQPSERVYMLA 900

Query: 901  TSHLNDSMNQTLQATETSLFSSSARQYMAP-AHRS-QLSLASHIDISSQLTSDRLYIS-- 960
            TSHLNDSM+QTLQ T+ S+FSSS R Y+ P +H++  +SLASH +++ Q TSDR+Y+S  
Sbjct: 901  TSHLNDSMSQTLQRTDASMFSSSERHYVPPTSHKNDHISLASHTEVALQSTSDRIYLSSL 960

Query: 961  ----------------------------PLSHRNECMPLKSEWLCIPTSAVTQRMYLQDS 1003
                                        PL HRN  MP KSEWLCIPT A  QRMY Q  
Sbjct: 961  TEREDNMASAASQQAESFTSTSDRMYIPPLVHRNAGMPPKSEWLCIPTPAAPQRMYPQGP 1020

BLAST of Sed0017008 vs. ExPASy TrEMBL
Match: A0A6J1GG15 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita moschata OX=3662 GN=LOC111453599 PE=3 SV=1)

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 809/1040 (77.79%), Postives = 878/1040 (84.42%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QS   L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSPTGLVPHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTI+NGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIRNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQL-VPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN S+ ANSSH VI D VK  HQL VPNLIGGEFLDS +CPVV+V NPATQEVV
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKHTHQLKVPNLIGGEFLDSHNCPVVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            SHVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV EQG
Sbjct: 241  SHVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVIEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDG+D +CIR+PIGVCAGICSFNH 
Sbjct: 301  KTLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGMDSYCIRDPIGVCAGICSFNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            AT+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTHD+IN+I
Sbjct: 361  ATVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHDIINYI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDDEDIKAIS ASS SA KNIYARAAATGK+VQS+ GGKSHAIIMPDANMEA LNALVDA
Sbjct: 421  CDDEDIKAISFASSCSAGKNIYARAAATGKQVQSHFGGKSHAIIMPDANMEATLNALVDA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGT+ QTCMAINIV+SVGSS+LW++KLVE AKALKVNVGTDP A LGPVITKEVKD  C
Sbjct: 481  GFGTSGQTCMAINIVVSVGSSILWKKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM Q
Sbjct: 541  RLVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            ADNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFNDKA
Sbjct: 601  ADNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFNDKA 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
            GLEFYT+LKRVAQQWKN   IGVSM   SPSERHLR+R  PS L+STS+KDSPGG+HRSL
Sbjct: 661  GLEFYTKLKRVAQQWKNSASIGVSMTASSPSERHLRSRTVPSTLVSTSDKDSPGGRHRSL 720

Query: 721  HPLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGD 780
             PLPST      SDAVLL +P+ISQTDL NERAT+SPPTP+RDLHG+GLSLIS LSS GD
Sbjct: 721  PPLPSTSERDSPSDAVLLPDPRISQTDLANERATSSPPTPDRDLHGQGLSLISTLSSEGD 780

Query: 781  ISNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHV 840
            ISN +LSPAM  TGDR+L  QAM      SSER+Y+ QK HWSE  RADS PS+SER   
Sbjct: 781  ISNQELSPAMLLTGDRELPGQAMSVSTSRSSERMYLSQKCHWSEPLRADSIPSSSER--- 840

Query: 841  PSSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSE 900
                   I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSE
Sbjct: 841  -------IKGQASRTTTPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSE 900

Query: 901  RGHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQL 960
            R +MLA SHLNDSM+QTL+ T++ LFSS                  S R Y++       
Sbjct: 901  RVYMLAASHLNDSMSQTLRRTDSPLFSSSDGKDHISLDSQTDAALQSDRMYLSSLSERDG 960

Query: 961  SLASHIDISSQ------LTSDRLYISPLSHRNECMPLKS-EWLCIPTSAVTQRMYLQDSI 1002
            ++AS    SSQ       TS+R+YI PL +RN  +P KS EWLCIPT A+++RMY Q  I
Sbjct: 961  NMAS---ASSQQVESLTSTSERMYIPPLGYRNAGIPPKSEEWLCIPTPALSERMYSQGPI 1020

BLAST of Sed0017008 vs. ExPASy TrEMBL
Match: A0A6J1IRA8 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita maxima OX=3661 GN=LOC111478665 PE=3 SV=1)

HSP 1 Score: 1534.2 bits (3971), Expect = 0.0e+00
Identity = 808/1033 (78.22%), Postives = 875/1033 (84.70%), Query Frame = 0

Query: 1    MGSQSQDELITQKKMLSPQPGRFEDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60
            MG+QSQ  L+  KKML PQPGRF+DREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD
Sbjct: 1    MGTQSQTGLVAHKKMLPPQPGRFDDREDLIKYVRDFGADQGYVVTIKKSRKDRRVILGCD 60

Query: 61   RGGVYRNRRKIDESRRKRKASSRLINCPFEAVGKKEDDTWILTIKNGDHNHEPLNDMSEH 120
            RGG+YRNRRKIDESRRKRKASSRLINCPFEA+GKKEDD W+LTIKNGDHNHEPL DMSEH
Sbjct: 61   RGGLYRNRRKIDESRRKRKASSRLINCPFEAIGKKEDDAWMLTIKNGDHNHEPLKDMSEH 120

Query: 121  PYSRRFTEDEVRQIKMMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSET 180
            PYSRRFTEDEVRQIK+MTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSE 
Sbjct: 121  PYSRRFTEDEVRQIKLMTEAGIKPRQVLKALKQHNPDLQSTPRHLYNLKAKIRQGNLSEK 180

Query: 181  NFKSWSPNSSVPANSSH-VIEDAVKQKHQL-VPNLIGGEFLDSRDCPVVEVTNPATQEVV 240
            NFKSW PN S+ ANSSH VI D VKQ HQL VPNLIGGEFLDS +CPVV+V NPATQEVV
Sbjct: 181  NFKSWRPNISLHANSSHTVIGDTVKQTHQLMVPNLIGGEFLDSHNCPVVDVINPATQEVV 240

Query: 241  SHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQG 300
            SHVPLTTYEEFKAAVNAAK+AFPSWKNTPISTRQ VMFKFQELI+RDMDKLV NIV+EQG
Sbjct: 241  SHVPLTTYEEFKAAVNAAKQAFPSWKNTPISTRQCVMFKFQELILRDMDKLVTNIVTEQG 300

Query: 301  KPLKDAQDDIICGLEVVKYACGLATMQMGEFIRNASDGIDYHCIREPIGVCAGICSFNHS 360
            K LKDAQ+DIICGLEVVK+ CGLATMQMGEF+ NASDGID +CIR+PIGVCAGICSFNH 
Sbjct: 301  KTLKDAQNDIICGLEVVKHVCGLATMQMGEFVPNASDGIDSYCIRDPIGVCAGICSFNHP 360

Query: 361  ATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMINFI 420
            AT+SLWMFP+AVTCGNTFVLKPCE  PGAS+LLAALAVEAGLPDGVLNIVHGTH++IN+I
Sbjct: 361  ATVSLWMFPVAVTCGNTFVLKPCETYPGASMLLAALAVEAGLPDGVLNIVHGTHEIINYI 420

Query: 421  CDDEDIKAISIASSSSAEKNIYARAAATGKKVQSNLGGKSHAIIMPDANMEAALNALVDA 480
            CDD DIKAIS ASS SA KNIYARAAATGK+VQS+LGGKSHAIIMPDANMEA LNALVDA
Sbjct: 421  CDDGDIKAISFASSCSAGKNIYARAAATGKQVQSHLGGKSHAIIMPDANMEATLNALVDA 480

Query: 481  GFGTARQTCMAINIVISVGSSVLWEEKLVEYAKALKVNVGTDPLAYLGPVITKEVKDHLC 540
            GFGT+ QTCMAINIV+SVGSS+LWE+KLVE AKALKVNVGTDP A LGPVITKEVKD  C
Sbjct: 481  GFGTSGQTCMAINIVVSVGSSILWEKKLVECAKALKVNVGTDPNADLGPVITKEVKDRFC 540

Query: 541  RLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVLLCMQQ 600
            RLV+SGI+ GARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEF GPVLLCM Q
Sbjct: 541  RLVQSGIEDGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFLGPVLLCM-Q 600

Query: 601  ADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINVAVPVPLPSSFNDKA 660
            ADNLEEAI+IVNRNKNRNGASIFTTSG+YARKFQSEVEVGMVGINVAV VPLPSSFN   
Sbjct: 601  ADNLEEAISIVNRNKNRNGASIFTTSGIYARKFQSEVEVGMVGINVAVTVPLPSSFN--- 660

Query: 661  GLEFYTQLKRVAQQWKNLPDIGVSMAIPSPSERHLRARATPSMLLSTSEKDSPGGQHRSL 720
            GLEFYT+LKRVAQQWKN   IGVSM   SPSE HLR R  PS L+STSEKDSPGG+HRSL
Sbjct: 661  GLEFYTKLKRVAQQWKNSAGIGVSMTASSPSESHLRPRTVPSTLVSTSEKDSPGGRHRSL 720

Query: 721  HPLPST------SDAVLLANPQISQTDLVNERATASPPTPERDLHGRGLSLISNLSSAGD 780
             PL ST      SDAVLL +P++SQTDL NERAT+SPP+P+RDLHG+GLSLIS LSS GD
Sbjct: 721  PPLSSTSERDSPSDAVLLPDPRLSQTDLANERATSSPPSPDRDLHGQGLSLISTLSSEGD 780

Query: 781  ISNHDLSPAMPSTGDRDLASQAM------SSERLYIPQKSHWSEVQRADSFPSTSERTHV 840
            ISN DLSPAM  TGDR+L  QAM      SSER++I QK HWSE  RADS PS+SER   
Sbjct: 781  ISNQDLSPAMLLTGDRELPGQAMSVSTSRSSERMHISQKCHWSESLRADSIPSSSER--- 840

Query: 841  PSSLTNSIIAQTSRTTHPALVLPAEALYAPTSSDSICQIIPGNDSTVPSRRIDSKCHSSE 900
                   I  Q SRTT PALVL AE  Y PTS DS C I  G+DSTVPSRRIDS C SSE
Sbjct: 841  -------IKGQASRTTPPALVLAAEGFYVPTSHDSNCLITHGSDSTVPSRRIDSMCQSSE 900

Query: 901  RGHMLATSHLNDSMNQTLQATETSLFSS------------------SARQYMAPAHRSQL 960
            R +MLATSHLNDSM+QTL+ T+T LFSS                  S R Y++       
Sbjct: 901  RVYMLATSHLNDSMSQTLRRTDTPLFSSSNGKDHISLDSQTDAALQSDRMYLSSLSERDG 960

Query: 961  SLASHIDISSQLTSDRLYISPLSHRNECMPLKSEWLCIPTSAVTQRMYLQDSIVSADEFQ 1002
            ++AS   ++S  TS+R+YI PL +RN  MP KSEWLCIPT A+++RMY Q  IVSADE Q
Sbjct: 961  NMASASSLTS--TSERMYIPPLGYRNAGMPPKSEWLCIPTPALSERMYSQGPIVSADESQ 1017

BLAST of Sed0017008 vs. TAIR 10
Match: AT2G14170.1 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 650.6 bits (1677), Expect = 2.1e-186
Identity = 313/496 (63.10%), Postives = 400/496 (80.65%), Query Frame = 0

Query: 210 VPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPIS 269
           VPNLIGG F++S+    ++V NPATQEVVS VPLTT EEFKAAV+AAK+AFP W+NTPI+
Sbjct: 114 VPNLIGGSFVESQSSSFIDVINPATQEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPIT 173

Query: 270 TRQHVMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEF 329
           TRQ VM KFQELI ++MDKL MNI +EQGK LKD+  DI  GLEVV++ACG+AT+QMGE+
Sbjct: 174 TRQRVMLKFQELIRKNMDKLAMNITTEQGKTLKDSHGDIFRGLEVVEHACGMATLQMGEY 233

Query: 330 IRNASDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASL 389
           + N S+G+D + IREP+GVCAGIC FN  A I LWMFP+AVTCGNTF+LKP EK+PGAS+
Sbjct: 234 LPNVSNGVDTYSIREPLGVCAGICPFNFPAMIPLWMFPVAVTCGNTFILKPSEKDPGASV 293

Query: 390 LLAALAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKK 449
           +LA LA+EAGLPDGVLNIVHGT+D +N ICDDEDI+A+S   S++A  +IYARAAA GK+
Sbjct: 294 ILAELAMEAGLPDGVLNIVHGTNDTVNAICDDEDIRAVSFVGSNTAGMHIYARAAAKGKR 353

Query: 450 VQSNLGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEY 509
           +QSN+G K+H +++PDAN++A LNAL+ AGFG A Q CMA++ V+ VG +  WE+KLVE 
Sbjct: 354 IQSNMGAKNHGLVLPDANIDATLNALLAAGFGAAGQRCMALSTVVFVGDAKSWEDKLVER 413

Query: 510 AKALKVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFV 569
           AKALKV  G++P A LGPVI+K+ K+ +CRL++SG+D GA+LLLDGRDIVVPGYE GNF+
Sbjct: 414 AKALKVTCGSEPDADLGPVISKQAKERICRLIQSGVDDGAKLLLDGRDIVVPGYEKGNFI 473

Query: 570 GPTILSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYAR 629
           GPTILS VT DMECYKEE FGPVL+CM QA++ +EAI+I+N+NK  NGA+IFT+SG  AR
Sbjct: 474 GPTILSGVTPDMECYKEEIFGPVLVCM-QANSFDEAISIINKNKYGNGAAIFTSSGAAAR 533

Query: 630 KFQSEVEVGMVGINVAVPVPLP--------------SSFNDKAGLEFYTQLKRVAQQWKN 689
           KFQ ++E G +GINV +PVPLP               +F  KAG++F+TQ+K V QQWK+
Sbjct: 534 KFQMDIEAGQIGINVPIPVPLPFFSFTGNKASFAGDLNFYGKAGVDFFTQIKTVTQQWKD 593

Query: 690 LPDIGVSMAIPSPSER 692
           +P   VS+A+P+  ++
Sbjct: 594 IP-TSVSLAMPTSQKQ 607

BLAST of Sed0017008 vs. TAIR 10
Match: AT2G14170.2 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 650.6 bits (1677), Expect = 2.1e-186
Identity = 313/496 (63.10%), Postives = 400/496 (80.65%), Query Frame = 0

Query: 210 VPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPIS 269
           VPNLIGG F++S+    ++V NPATQEVVS VPLTT EEFKAAV+AAK+AFP W+NTPI+
Sbjct: 5   VPNLIGGSFVESQSSSFIDVINPATQEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPIT 64

Query: 270 TRQHVMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEF 329
           TRQ VM KFQELI ++MDKL MNI +EQGK LKD+  DI  GLEVV++ACG+AT+QMGE+
Sbjct: 65  TRQRVMLKFQELIRKNMDKLAMNITTEQGKTLKDSHGDIFRGLEVVEHACGMATLQMGEY 124

Query: 330 IRNASDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASL 389
           + N S+G+D + IREP+GVCAGIC FN  A I LWMFP+AVTCGNTF+LKP EK+PGAS+
Sbjct: 125 LPNVSNGVDTYSIREPLGVCAGICPFNFPAMIPLWMFPVAVTCGNTFILKPSEKDPGASV 184

Query: 390 LLAALAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKK 449
           +LA LA+EAGLPDGVLNIVHGT+D +N ICDDEDI+A+S   S++A  +IYARAAA GK+
Sbjct: 185 ILAELAMEAGLPDGVLNIVHGTNDTVNAICDDEDIRAVSFVGSNTAGMHIYARAAAKGKR 244

Query: 450 VQSNLGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEY 509
           +QSN+G K+H +++PDAN++A LNAL+ AGFG A Q CMA++ V+ VG +  WE+KLVE 
Sbjct: 245 IQSNMGAKNHGLVLPDANIDATLNALLAAGFGAAGQRCMALSTVVFVGDAKSWEDKLVER 304

Query: 510 AKALKVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFV 569
           AKALKV  G++P A LGPVI+K+ K+ +CRL++SG+D GA+LLLDGRDIVVPGYE GNF+
Sbjct: 305 AKALKVTCGSEPDADLGPVISKQAKERICRLIQSGVDDGAKLLLDGRDIVVPGYEKGNFI 364

Query: 570 GPTILSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYAR 629
           GPTILS VT DMECYKEE FGPVL+CM QA++ +EAI+I+N+NK  NGA+IFT+SG  AR
Sbjct: 365 GPTILSGVTPDMECYKEEIFGPVLVCM-QANSFDEAISIINKNKYGNGAAIFTSSGAAAR 424

Query: 630 KFQSEVEVGMVGINVAVPVPLP--------------SSFNDKAGLEFYTQLKRVAQQWKN 689
           KFQ ++E G +GINV +PVPLP               +F  KAG++F+TQ+K V QQWK+
Sbjct: 425 KFQMDIEAGQIGINVPIPVPLPFFSFTGNKASFAGDLNFYGKAGVDFFTQIKTVTQQWKD 484

Query: 690 LPDIGVSMAIPSPSER 692
           +P   VS+A+P+  ++
Sbjct: 485 IP-TSVSLAMPTSQKQ 498

BLAST of Sed0017008 vs. TAIR 10
Match: AT2G14170.3 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 624.4 bits (1609), Expect = 1.6e-178
Identity = 303/471 (64.33%), Postives = 382/471 (81.10%), Query Frame = 0

Query: 210 VPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPIS 269
           VPNLIGG F++S+    ++V NPATQEVVS VPLTT EEFKAAV+AAK+AFP W+NTPI+
Sbjct: 114 VPNLIGGSFVESQSSSFIDVINPATQEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPIT 173

Query: 270 TRQHVMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEF 329
           TRQ VM KFQELI ++MDKL MNI +EQGK LKD+  DI  GLEVV++ACG+AT+QMGE+
Sbjct: 174 TRQRVMLKFQELIRKNMDKLAMNITTEQGKTLKDSHGDIFRGLEVVEHACGMATLQMGEY 233

Query: 330 IRNASDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASL 389
           + N S+G+D + IREP+GVCAGIC FN  A I LWMFP+AVTCGNTF+LKP EK+PGAS+
Sbjct: 234 LPNVSNGVDTYSIREPLGVCAGICPFNFPAMIPLWMFPVAVTCGNTFILKPSEKDPGASV 293

Query: 390 LLAALAVEAGLPDGVLNIVHGTHDMINFICDDEDIKAISIASSSSAEKNIYARAAATGKK 449
           +LA LA+EAGLPDGVLNIVHGT+D +N ICDDEDI+A+S   S++A  +IYARAAA GK+
Sbjct: 294 ILAELAMEAGLPDGVLNIVHGTNDTVNAICDDEDIRAVSFVGSNTAGMHIYARAAAKGKR 353

Query: 450 VQSNLGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAINIVISVGSSVLWEEKLVEY 509
           +QSN+G K+H +++PDAN++A LNAL+ AGFG A Q CMA++ V+ VG +  WE+KLVE 
Sbjct: 354 IQSNMGAKNHGLVLPDANIDATLNALLAAGFGAAGQRCMALSTVVFVGDAKSWEDKLVER 413

Query: 510 AKALKVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFV 569
           AKALKV  G++P A LGPVI+K+ K+ +CRL++SG+D GA+LLLDGRDIVVPGYE GNF+
Sbjct: 414 AKALKVTCGSEPDADLGPVISKQAKERICRLIQSGVDDGAKLLLDGRDIVVPGYEKGNFI 473

Query: 570 GPTILSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYAR 629
           GPTILS VT DMECYKEE FGPVL+CM QA++ +EAI+I+N+NK  NGA+IFT+SG  AR
Sbjct: 474 GPTILSGVTPDMECYKEEIFGPVLVCM-QANSFDEAISIINKNKYGNGAAIFTSSGAAAR 533

Query: 630 KFQSEVEVGMVGINVAVPVPLP--SSFNDKAG----LEFYTQLKRVAQQWK 675
           KFQ ++E G +GINV +PVPLP  S   +KA     L FY ++    +Q K
Sbjct: 534 KFQMDIEAGQIGINVPIPVPLPFFSFTGNKASFAGDLNFYGKILVTTKQKK 583

BLAST of Sed0017008 vs. TAIR 10
Match: AT1G79440.1 (aldehyde dehydrogenase 5F1 )

HSP 1 Score: 192.2 bits (487), Expect = 2.0e-48
Identity = 130/433 (30.02%), Postives = 213/433 (49.19%), Query Frame = 0

Query: 213 LIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEEFKAAVNAAKRAFPSWKNTPISTRQ 272
           LIGG++LDS D   ++V NPAT E+++ V     +E   A+ ++  AF SW       R 
Sbjct: 56  LIGGKWLDSYDNKTIKVNNPATGEIIADVACMGTKETNDAIASSYEAFTSWSRLTAGERS 115

Query: 273 HVMFKFQELIIRDMDKLVMNIVSEQGKPLKDAQDDIICGLEVVKYACGLATMQMGEFIRN 332
            V+ ++ +L+I   ++L   I  EQGKPLK+A  ++  G   ++Y    A    G+ I  
Sbjct: 116 KVLRRWYDLLIAHKEELGQLITLEQGKPLKEAIGEVAYGASFIEYYAEEAKRVYGDIIPP 175

Query: 333 ASDGIDYHCIREPIGVCAGICSFNHSATISLWMFPIAVTCGNTFVLKPCEKNPGASLLLA 392
                    +++P+GV   I  +N    +       A+  G T V+KP E  P  +L  A
Sbjct: 176 NLSDRRLLVLKQPVGVVGAITPWNFPLAMITRKVGPALASGCTVVVKPSELTPLTALAAA 235

Query: 393 ALAVEAGLPDGVLNIVHGTHDMI-NFICDDEDIKAISIASSSSAEKNIYARAAATGKKVQ 452
            LA++AG+P G LN+V G    I + +     ++ I+   S++  K + A AA T KKV 
Sbjct: 236 ELALQAGVPPGALNVVMGNAPEIGDALLTSPQVRKITFTGSTAVGKKLMAAAAPTVKKVS 295

Query: 453 SNLGGKSHAIIMPDANMEAALNALVDAGFGTARQTCMAIN-IVISVGSSVLWEEKLVEYA 512
             LGG + +I+  DA+++ A+   + A F  + QTC+  N +++  G    + E   E  
Sbjct: 296 LELGGNAPSIVFDDADLDVAVKGTLAAKFRNSGQTCVCANRVLVQDGIYDKFAEAFSEAV 355

Query: 513 KALKVNVGTDPLAYLGPVITKEVKDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFVG 572
           + L+V  G       GP+I       +   V+  +  GA++++ G+   +       F  
Sbjct: 356 QKLEVGDGFRDGTTQGPLINDAAVQKVETFVQDAVSKGAKIIIGGKRHSL----GMTFYE 415

Query: 573 PTILSDVTTDMECYKEEFFGPVLLCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYARK 632
           PT++ DV+ +M   KEE FGPV   ++     E+AI I N       A IFT S   + +
Sbjct: 416 PTVIRDVSDNMIMSKEEIFGPVAPLIRFKTE-EDAIRIANDTIAGLAAYIFTNSVQRSWR 475

Query: 633 FQSEVEVGMVGIN 644
               +E G+VG+N
Sbjct: 476 VFEALEYGLVGVN 483

BLAST of Sed0017008 vs. TAIR 10
Match: AT1G23800.1 (aldehyde dehydrogenase 2B7 )

HSP 1 Score: 177.2 bits (448), Expect = 6.7e-44
Identity = 155/513 (30.21%), Postives = 233/513 (45.42%), Query Frame = 0

Query: 189 SSVPANSSHVIEDAVKQKHQLVPNLIGGEFLDSRDCPVVEVTNPATQEVVSHVPLTTYEE 248
           S++ A   + I   VK +H  +  LIGG F+D+         +P   EV++ V     E+
Sbjct: 35  SNLAAAVENTITPPVKVEHTQL--LIGGRFVDAVSGKTFPTLDPRNGEVIAQVSEGDAED 94

Query: 249 FKAAVNAAKRAFPS--WKNTPISTRQHVMFKFQELIIRDMDKLVMNIVSEQGKPL-KDAQ 308
              AV AA++AF    W       R  ++F+F +LI +  D++      + GKP  + AQ
Sbjct: 95  VNRAVAAARKAFDEGPWPKMTAYERSKILFRFADLIEKHNDEIAALETWDNGKPYEQSAQ 154

Query: 309 DDIICGLEVVKYACGLATMQMGEFIRNASDGIDY-HCIREPIGVCAGICSFNHSATISLW 368
            ++     V +Y  G A    G  +    DG  +   + EPIGV   I  +N    +  W
Sbjct: 155 IEVPMLARVFRYYAGWADKIHG--MTMPGDGPHHVQTLHEPIGVAGQIIPWNFPLLMLSW 214

Query: 369 MFPIAVTCGNTFVLKPCEKNPGASLLLAALAVEAGLPDGVLNIVHGTHDMIN-FICDDED 428
               A+ CGNT VLK  E+ P ++LL+  L  EAGLPDGV+NIV G        I    D
Sbjct: 215 KLGPALACGNTVVLKTAEQTPLSALLVGKLLHEAGLPDGVVNIVSGFGATAGAAIASHMD 274

Query: 429 IKAISIASSSSAEKNIYARAAATG-KKVQSNLGGKSHAIIMPDANMEAALNALVDAGFGT 488
           +  ++   S+   K I   A+ +  K V   LGGKS  I+  DA+++ A+     A F  
Sbjct: 275 VDKVAFTGSTDVGKIILELASKSNLKAVTLELGGKSPFIVCEDADVDQAVELAHFALFFN 334

Query: 489 ARQTCMAINIVISVGSSVLWEEKLVE------YAKALKVNVGTDPL---AYLGPVITKEV 548
             Q C A       GS     E++ +       A+ALK NVG DP       GP +  E 
Sbjct: 335 QGQCCCA-------GSRTFVHERVYDEFVEKAKARALKRNVG-DPFKSGIEQGPQVDSEQ 394

Query: 549 KDHLCRLVRSGIDSGARLLLDGRDIVVPGYENGNFVGPTILSDVTTDMECYKEEFFGPVL 608
            + + + ++ G+++GA L   G  +   GY    ++ PT+ SDV  DM    +E FGPV 
Sbjct: 395 FNKILKYIKHGVEAGATLQAGGDRLGSKGY----YIQPTVFSDVKDDMLIATDEIFGPVQ 454

Query: 609 LCMQQADNLEEAIAIVNRNKNRNGASIFTTSGVYARKFQSEVEVGMVGINV--AVPVPLP 668
             ++  D L+E IA  N ++    A +FT +   A +    + VG V IN    +   +P
Sbjct: 455 TILKFKD-LDEVIARANNSRYGLAAGVFTQNLDTAHRLMRALRVGTVWINCFDVLDASIP 514

Query: 669 SSFNDKAG---------LEFYTQLKRVAQQWKN 676
                 +G         L  Y Q+K V    KN
Sbjct: 515 FGGYKMSGIGREKGIYSLNNYLQVKAVVTSLKN 530