Sed0016413 (gene) Chayote v1

Overview


Name	Sed0016413
Type	gene
Organism	Sechium edule (Chayote v1)
Description	E3 ubiquitin-protein ligase EL5
Location	LG04: 43793768 .. 43796260 (+)
RNA-Seq Expression	Sed0016413
Synteny	Sed0016413

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTCCTAAATTGAAATCTCCAAGCACCTCAAAAAAGTAAATCTATAATGGAGTGGGAATCTGACTATAGTCTTTTACGTGAGAATACAATAACCAAGCAAAAAAAAGCATGACAAAAAGAAACAAAAAGGACGGATAAAAGATGATGGTATGGAGGAGTATATATTCACTTGGATTAGCTACACATCTTCATGGAGTGAAAGAGCTATAAAATCCAGAACACTGAAAACCAACAATTTAAGATCTAAAAGATTAAAGAAGAAACTTATAAAAAAATGAGCAGACGTTCTACTAATTTTCCTCCAGGGATTTGGCAGACTAGAAGGCAATATGATCGCCAGAGGTATAACAGAAATGATTCAAACCATATGAACTCCACACCGCCACACCCTCGTCAATTTCAGGTAAGCAGGTTAACAATTCTTTTTTTTTTTTTGCAATGAACCAACTGTATTCTTTTTTTATAATCGGGAATCGAGTTTCACTACTCGGTGCACCCGTGCGTGTCCATTCCAAGGTCCGGACACAAGAGACAACAAAGAATTTTACAATTAAGCTCACTAGTGGGTTTGGATCTCCACGTAGAGGTTGTACCTTGGAGACCAATGAACTGTATTCAAACATGTCATCAAAGCTTAAGCTATTACCCTACAACATGATTTGCTGGTGGCAAGGCAGAACTCTCATTTCACATTTAAATTCTATGTTAATAGTTCTAGTTTTCTCAAAAATGTATTAGTGGTCGGGAAGTTAAAAAAAAATGGTAGCAATAGTATACCTTATTATTCATGTGTTGACAGAATTCTCATAGAGAGATAGATCAAGTTCCATGGAGGCCCACTGGAGAGATAGATGCAATTCCGTGGCAGCCTGCGGGTGAGACAATGATTACAAGAATATGGGACATTGGCGGGTAAATAACTCAAAACCTTAGTAGTTTTTAGGCGCTGTTTAGTGTTATGAACTGAATTATATGCAAACCAAAGAAATTGATATTGCAGATTCATTTCATAAAAAGGTCTAAAATTACAAGAGTTTAGAGGCCTACCAAAGTTTGTGGTATATTGCCTGACACAAACTAGTATTTAACTCAGAAAGATAACTTAACAGAGTATTCAGAAGTCCATGAATTTCAATAATGGAAGCAATTGAGGAAACAAATCATGAAGTGTCAGAAAGGGGGTTGAGCATCAATTAAGCTTAGTTGGAAACTGGTTTGTTGGAGATTCTTTTACACAAAGAAATTCAACATGTTTAATAACCAAGAAATCGAACCTATTACGTGTAGACAACAATTTAGCTTAATAGGTTGGCCCAACTATTCAACAACATTCTTTTGGTCCTACGATATTTTCCAGTGCCACACAGCCAGTGGTGCCCCTAGGTTTAGAGGAGCAAAGCTTCAAGTTCCCGAGTCATAAAAAAAAACCAATTCAACAACATTCAACACCACAAATAGCATTAAGCATAAATTTGATCACCAAAAATCTATAACTACAAGTTGCAGGGAAAAATAAACATGTCATCTCATTCAAAAAATTTCATGAGCAGCATCCAAATTTGCAATAACAAACTAGTCCCCTTTGACATTATCAGTTGGTTTAGAGAAAATCTACCTACATTGCTCTTGTATCCTGCAATTAAATTCCAAATCCTCCAAACTGTGCTGCTAATTCATTCACTGAAATTTCCAGCCCGCCAGAAGAACCACCTGCCATGGTCAGAATCATTACAGACTCAAGATCTGGAGAGCTGTACACGCCATCTCCAATATCCGAAAGGAACTCGACGCTAACATCTGGAGAGCAAAATACAGCACTGAATAAGCTGAAGAAAAAAGTGTACGATCCTCCAAACAACACAAATGGGAGAACAAGGTTCCATTACAGAGAGAACAACAAAGGAACTTACAGCGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTGGAAGATTTCCGGCGAAAAGAGGAAGTGACGACGACGCCGTGCAACCATATGTTTCACGAAGAATGCATAGTGCCGTGGGTGAAGAGCCATGGCCAGTGTCCGAATTGCAGATCCGCCTTGTTCGAAAGAACGCGGCGAGGAGGAGCTTCGTCCGGTAACACTGTATCGATTGGGCGGCCGGCTAGTGGCTTCGTCGGCGACGAGCTTCTGTACTTCATACGCGCCATGGAAGATGCTCTGATGCTGCGAAGTATGGCCGGATTTTGGGGAGCTTGAACCGAAATCAACATGGAGAATGTCGTGAATTTCAGGAAATTATGAAATACGATCTCTGAAATTTGTTTTGACATTTGAAACGATAGAGCTCGATTTTAACCTAACCATGAAAGCATTCTCTCGTTTTGATCTTAATCTTGATCTTGGCGGTATAATTCTTCGCGGGCATTACTAGATAGAATGTACTGATTATGACTCATTATATAGTTAATAAAAATAAAGTCATTATATAGTTTTTGAGATCAAAGTCGTTAAAAATATTTTAAATT

mRNA sequence

TTTCTCCTAAATTGAAATCTCCAAGCACCTCAAAAAAGTAAATCTATAATGGAGTGGGAATCTGACTATAGTCTTTTACGTGAGAATACAATAACCAAGCAAAAAAAAGCATGACAAAAAGAAACAAAAAGGACGGATAAAAGATGATGGTATGGAGGAGTATATATTCACTTGGATTAGCTACACATCTTCATGGAGTGAAAGAGCTATAAAATCCAGAACACTGAAAACCAACAATTTAAGATCTAAAAGATTAAAGAAGAAACTTATAAAAAAATGAGCAGACGTTCTACTAATTTTCCTCCAGGGATTTGGCAGACTAGAAGGCAATATGATCGCCAGAGGTATAACAGAAATGATTCAAACCATATGAACTCCACACCGCCACACCCTCGTCAATTTCAGAATTCTCATAGAGAGATAGATCAAGTTCCATGGAGGCCCACTGGAGAGATAGATGCAATTCCGTGGCAGCCTGCGGGTGAGACAATGATTACAAGAATATGGGACATTGGCGGCCCGCCAGAAGAACCACCTGCCATGGTCAGAATCATTACAGACTCAAGATCTGGAGAGCTGTACACGCCATCTCCAATATCCGAAAGGAACTCGACGCTAACATCTGGAGAGCAAAATACAGCACTGAATAAGCTGAAGAAAAAAGTGTACGATCCTCCAAACAACACAAATGGGAGAACAAGGTTCCATTACAGAGAGAACAACAAAGGAACTTACAGCGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTGGAAGATTTCCGGCGAAAAGAGGAAGTGACGACGACGCCGTGCAACCATATGTTTCACGAAGAATGCATAGTGCCGTGGGTGAAGAGCCATGGCCAGTGTCCGAATTGCAGATCCGCCTTGTTCGAAAGAACGCGGCGAGGAGGAGCTTCGTCCGGTAACACTGTATCGATTGGGCGGCCGGCTAGTGGCTTCGTCGGCGACGAGCTTCTGTACTTCATACGCGCCATGGAAGATGCTCTGATGCTGCGAAGTATGGCCGGATTTTGGGGAGCTTGAACCGAAATCAACATGGAGAATGTCGTGAATTTCAGGAAATTATGAAATACGATCTCTGAAATTTGTTTTGACATTTGAAACGATAGAGCTCGATTTTAACCTAACCATGAAAGCATTCTCTCGTTTTGATCTTAATCTTGATCTTGGCGGTATAATTCTTCGCGGGCATTACTAGATAGAATGTACTGATTATGACTCATTATATAGTTAATAAAAATAAAGTCATTATATAGTTTTTGAGATCAAAGTCGTTAAAAATATTTTAAATT

Coding sequence (CDS)

ATGAGCAGACGTTCTACTAATTTTCCTCCAGGGATTTGGCAGACTAGAAGGCAATATGATCGCCAGAGGTATAACAGAAATGATTCAAACCATATGAACTCCACACCGCCACACCCTCGTCAATTTCAGAATTCTCATAGAGAGATAGATCAAGTTCCATGGAGGCCCACTGGAGAGATAGATGCAATTCCGTGGCAGCCTGCGGGTGAGACAATGATTACAAGAATATGGGACATTGGCGGCCCGCCAGAAGAACCACCTGCCATGGTCAGAATCATTACAGACTCAAGATCTGGAGAGCTGTACACGCCATCTCCAATATCCGAAAGGAACTCGACGCTAACATCTGGAGAGCAAAATACAGCACTGAATAAGCTGAAGAAAAAAGTGTACGATCCTCCAAACAACACAAATGGGAGAACAAGGTTCCATTACAGAGAGAACAACAAAGGAACTTACAGCGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTGGAAGATTTCCGGCGAAAAGAGGAAGTGACGACGACGCCGTGCAACCATATGTTTCACGAAGAATGCATAGTGCCGTGGGTGAAGAGCCATGGCCAGTGTCCGAATTGCAGATCCGCCTTGTTCGAAAGAACGCGGCGAGGAGGAGCTTCGTCCGGTAACACTGTATCGATTGGGCGGCCGGCTAGTGGCTTCGTCGGCGACGAGCTTCTGTACTTCATACGCGCCATGGAAGATGCTCTGATGCTGCGAAGTATGGCCGGATTTTGGGGAGCTTGA

Protein sequence

MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEIDAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSRSGELYTPSPISERNSTLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTYSDEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGASSGNTVSIGRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA

Homology

[Close]

BLAST of Sed0016413 vs. NCBI nr
Match: XP_038888228.1 (uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida])

HSP 1 Score: 355.1 bits (910), Expect = 4.9e-94
Identity = 187/274 (68.25%), Postives = 205/274 (74.82%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR+YDRQRYNRNDSN +NSTP H   FQNS+RE+D VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRINSTPSHTNSFQNSYREVDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGETMI RI DIG P EEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETMIARILDIGRPREEPPAIVRIITSSRPRQLEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY-------SDEEGKRCAVC 180
           STLTS EQN AL KLKK VYDPP+NT+ R  F+YRENN+G Y       SDEEGKRCAVC
Sbjct: 121 STLTSEEQNMALTKLKKNVYDPPSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGAS--SGNTVSI 240
           LEDF+ KEEV TTPCNHMFHEECIVPWVKS+GQCP CR  LFERT R GAS  S NTVS 
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTERRGASSNSNNTVST 240

Query: 241 GRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA 257
             P S  + D+L+Y IRAMEDALMLR++AGFWGA
Sbjct: 241 A-PPSSLIDDDLMYLIRAMEDALMLRNLAGFWGA 262

[Close]

BLAST of Sed0016413 vs. NCBI nr
Match: XP_022989128.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita maxima])

HSP 1 Score: 347.4 bits (890), Expect = 1.0e-91
Identity = 181/282 (64.18%), Postives = 204/282 (72.34%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHRE-----------I 60
           MSRRSTNFPPG+W+ RR+YDRQRYN NDSN M+ST PHP+ FQNS R+           I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DQVPWRPTGEIDAIPWQPAGETMITRIWDIGGPPEEPPAMVRIIT---------DSRSGE 120
           D VP RPT E+D IPW+P G  MI R+ +I G  E+PPA+VRIIT          SR  E
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LYTPSPISERNSTLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY------- 180
           LY P+P SE NSTLTS EQN ALNKLKKKVYDPPNN + R  F+YRENN+  Y       
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 SDEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGG 240
           +DE+GKRCAVCLEDF+ KEEV TTPCNHMFHEECIVPWVKSHGQCPNCR  LFERT R  
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSGNTVSIGRPASGFVGDELLYFIRAMEDALMLRSMAGFWG 256
           ASS NT SI    S  V DEL+Y IRAMED+LMLR++ G WG
Sbjct: 241 ASSNNTASIA-SGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Sed0016413 vs. NCBI nr
Match: XP_008455285.1 (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 345.5 bits (885), Expect = 3.9e-91
Identity = 187/275 (68.00%), Postives = 206/275 (74.91%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR+YDRQRYNRN+SN +NSTP H + FQNS+REID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGETMITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY-------SDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+  Y       SDEEGK CAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFE-RTRRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKS+GQCP CR  LFE RT R GASS   N VS
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNNVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA 257
           I  P S  V DEL+Y IRAME+ALMLR++A FWGA
Sbjct: 241 IA-PPSSLVDDELMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Sed0016413 vs. NCBI nr
Match: XP_023529368.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344.7 bits (883), Expect = 6.7e-91
Identity = 179/282 (63.48%), Postives = 203/282 (71.99%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHRE-----------I 60
           MSRRSTNFPPG+W+ RR+YD QRYN NDSN M+ST PHP+ FQNS R+           I
Sbjct: 1   MSRRSTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPLRPTGGI 60

Query: 61  DQVPWRPTGEIDAIPWQPAGETMITRIWDIGGPPEEPPAMVRIIT---------DSRSGE 120
           D VP RPT E+D IPW+P G  MI R+ +I G  E+PPA+VRIIT          SR  E
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LYTPSPISERNSTLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY------- 180
           +Y P+P SE NSTLTS EQN ALNKLKKKVYDPPNN + R  F+YRENN+  Y       
Sbjct: 121 MYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRKNYNERSNGE 180

Query: 181 SDEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGG 240
           +DE+GKRCAVCLEDF+ KEEV TTPCNHMFHEECIVPWVKSHGQCPNCR  LFERT R  
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSGNTVSIGRPASGFVGDELLYFIRAMEDALMLRSMAGFWG 256
           ASS NT SI    S  V DEL+Y IRAMED+LMLR++ G WG
Sbjct: 241 ASSNNTASIA-SGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Sed0016413 vs. NCBI nr
Match: XP_004136838.1 (E3 ubiquitin-protein ligase AIRP1 [Cucumis sativus] >KGN43640.1 hypothetical protein Csa_017086 [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 1.9e-90
Identity = 184/275 (66.91%), Postives = 207/275 (75.27%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR++DRQRYNRN+SN  NSTP H + FQNS+R+ID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGET+ITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNK-------GTYSDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+        +  DEEGKRCAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNGDEEGKRCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERT-RRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKSHGQCP CR  LFERT RRG ASS   N+VS
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNSVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA 257
           I  P S  V +EL+Y IRAME+ALMLR++A FWGA
Sbjct: 241 IA-PPSSLVDEELVYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-10
Identity = 26/48 (54.17%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 158 GKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL 206
           G  C VC ED+  +EEV   PCNH FH  CIVPW++ H  CP CR +L
Sbjct: 225 GLECPVCKEDYTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSL 272

[Close]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot
Match: Q91YL2 (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-10
Identity = 25/48 (52.08%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 158 GKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL 206
           G  C VC ED+   E V   PCNH+FH+ CIVPW++ H  CP CR +L
Sbjct: 228 GLECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCRKSL 275

[Close]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.1e-10
Identity = 29/72 (40.28%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 155 DEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL------FER 214
           + E  +CAVC++DF    E    PC H++H++C++PW++ H  CP CR  L      +ER
Sbjct: 218 NSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYER 277

Query: 215 TRRG--GASSGN 219
             RG  G S GN
Sbjct: 278 RVRGAQGTSGGN 289

[Close]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot
Match: Q6IRP0 (E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.5e-10
Identity = 24/48 (50.00%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 158 GKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL 206
           G  C VC ED+   E V   PCNH+FH +CI+PW++ H  CP CR +L
Sbjct: 225 GLECPVCKEDYTVGESVRQLPCNHLFHNDCIIPWLEQHDTCPVCRKSL 272

[Close]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.5e-10
Identity = 30/69 (43.48%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 158 GKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL-----FERTRRG 217
           G  C VC ED+  +E+V   PCNH FH  CIVPW++ H  CP CR +L       +T+  
Sbjct: 226 GLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSS 285

Query: 218 GASSGNTVS 222
            AS+ N  S
Sbjct: 286 EASASNRFS 294

[Close]

BLAST of Sed0016413 vs. ExPASy TrEMBL
Match: A0A6J1JPC8 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 5.0e-92
Identity = 181/282 (64.18%), Postives = 204/282 (72.34%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHRE-----------I 60
           MSRRSTNFPPG+W+ RR+YDRQRYN NDSN M+ST PHP+ FQNS R+           I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DQVPWRPTGEIDAIPWQPAGETMITRIWDIGGPPEEPPAMVRIIT---------DSRSGE 120
           D VP RPT E+D IPW+P G  MI R+ +I G  E+PPA+VRIIT          SR  E
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LYTPSPISERNSTLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY------- 180
           LY P+P SE NSTLTS EQN ALNKLKKKVYDPPNN + R  F+YRENN+  Y       
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 SDEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGG 240
           +DE+GKRCAVCLEDF+ KEEV TTPCNHMFHEECIVPWVKSHGQCPNCR  LFERT R  
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSGNTVSIGRPASGFVGDELLYFIRAMEDALMLRSMAGFWG 256
           ASS NT SI    S  V DEL+Y IRAMED+LMLR++ G WG
Sbjct: 241 ASSNNTASIA-SGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Sed0016413 vs. ExPASy TrEMBL
Match: A0A1S3C054 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495488 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.9e-91
Identity = 187/275 (68.00%), Postives = 206/275 (74.91%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR+YDRQRYNRN+SN +NSTP H + FQNS+REID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGETMITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY-------SDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+  Y       SDEEGK CAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFE-RTRRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKS+GQCP CR  LFE RT R GASS   N VS
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNNVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA 257
           I  P S  V DEL+Y IRAME+ALMLR++A FWGA
Sbjct: 241 IA-PPSSLVDDELMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Sed0016413 vs. ExPASy TrEMBL
Match: A0A0A0K1V1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 9.4e-91
Identity = 184/275 (66.91%), Postives = 207/275 (75.27%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR++DRQRYNRN+SN  NSTP H + FQNS+R+ID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGET+ITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNK-------GTYSDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+        +  DEEGKRCAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNGDEEGKRCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERT-RRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKSHGQCP CR  LFERT RRG ASS   N+VS
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNSVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFWGA 257
           I  P S  V +EL+Y IRAME+ALMLR++A FWGA
Sbjct: 241 IA-PPSSLVDEELVYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Sed0016413 vs. ExPASy TrEMBL
Match: A0A5A7SLQ9 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003130 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.6e-90
Identity = 185/273 (67.77%), Postives = 205/273 (75.09%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR+YDRQRYNRN+SN +NSTP H + FQNS+REID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGETMITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY-------SDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+  Y       SDEEGK CAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFE-RTRRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKS+GQCP CR  LFE RT R GASS   N+VS
Sbjct: 181 LEDFQSKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNSVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFW 255
           I  P S  V DEL+Y IRAME+ALMLR++A FW
Sbjct: 241 IA-PPSSLVDDELMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of Sed0016413 vs. ExPASy TrEMBL
Match: A0A5D3C526 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003170 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 2.1e-90
Identity = 185/273 (67.77%), Postives = 205/273 (75.09%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQTRRQYDRQRYNRNDSNHMNSTPPHPRQFQNSHREIDQVPWRPTGEI 60
           MSRRSTNFPPGIWQ RR+YDRQRYNRN+SN +NSTP H + FQNS+REID VPWR     
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWR----- 60

Query: 61  DAIPWQPAGETMITRIWDIGGPPEEPPAMVRIITDSR---------SGELYTPSPISERN 120
                 PAGETMITRI DIG  PEEPPA+VRIIT SR           ELY+P+P SE N
Sbjct: 61  ------PAGETMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGN 120

Query: 121 STLTSGEQNTALNKLKKKVYDPPNNTNGRTRFHYRENNKGTY-------SDEEGKRCAVC 180
           STLTS EQN ALNKLKKKVYDPP+NT+ R  F+YRENN+  Y       SDEEGK CAVC
Sbjct: 121 STLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVC 180

Query: 181 LEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFE-RTRRGGASS--GNTVS 240
           LEDF+ KEEV TTPCNHMFHE+CIVPWVKS+GQCP CR  LFE RT R GASS   N+VS
Sbjct: 181 LEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNSVS 240

Query: 241 IGRPASGFVGDELLYFIRAMEDALMLRSMAGFW 255
           I  P S  V DEL+Y IRAME+ALMLR++A FW
Sbjct: 241 IA-PPSSLVDDELMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of Sed0016413 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 3.7e-23
Identity = 60/154 (38.96%), Postives = 89/154 (57.79%), Query Frame = 0


Query: 106 PISERNSTLTSGEQNTALNKLKKKVYDP-PNNTNGRT-RFHYRENNKGTYS--------- 165
           P S   S+  + E++  L  L K+ Y+P P +T  R+   +YR  N G+ S         
Sbjct: 78  PESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNLSG 137

Query: 166 DEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGA 225
           +E+ KRC+VCLEDF  KE V  TPC HMFHEECIVPW+K+ GQCP CR  + + T++  +
Sbjct: 138 EEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLKTKGQCPVCRFVILKPTKQDSS 197

Query: 226 --SSGNTVSIGRPASGFVGDELLYFIRAMEDALM 247
             ++G+ ++     +     ELL  +RAME+  +
Sbjct: 198 PPANGSNLTGDMTTNDLFTLELLSVVRAMEETFL 231

[Close]

BLAST of Sed0016413 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 3.7e-23
Identity = 60/154 (38.96%), Postives = 89/154 (57.79%), Query Frame = 0


Query: 106 PISERNSTLTSGEQNTALNKLKKKVYDP-PNNTNGRT-RFHYRENNKGTYS--------- 165
           P S   S+  + E++  L  L K+ Y+P P +T  R+   +YR  N G+ S         
Sbjct: 106 PESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNLSG 165

Query: 166 DEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGA 225
           +E+ KRC+VCLEDF  KE V  TPC HMFHEECIVPW+K+ GQCP CR  + + T++  +
Sbjct: 166 EEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLKTKGQCPVCRFVILKPTKQDSS 225

Query: 226 --SSGNTVSIGRPASGFVGDELLYFIRAMEDALM 247
             ++G+ ++     +     ELL  +RAME+  +
Sbjct: 226 PPANGSNLTGDMTTNDLFTLELLSVVRAMEETFL 259

[Close]

BLAST of Sed0016413 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.5e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 0


Query: 154 SDEEGKRCAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSAL 206
           SD+    CA+C EDF   E     PCNH++H +CI+PW+ SH  CP CR  L
Sbjct: 89  SDDSALPCAICREDFVVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVEL 140

[Close]

BLAST of Sed0016413 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 7.3e-11
Identity = 28/68 (41.18%), Postives = 37/68 (54.41%), Query Frame = 0


Query: 161 CAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGASSGNTV 220
           C VC ++F  K E    PC+H++H +CIVPW+  H  CP CR  L  R    G+SS    
Sbjct: 186 CPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR----GSSSSTQS 245

Query: 221 SIGRPASG 229
           S  R  +G
Sbjct: 246 SQNRSTNG 249

[Close]

BLAST of Sed0016413 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 7.3e-11
Identity = 28/68 (41.18%), Postives = 37/68 (54.41%), Query Frame = 0


Query: 161 CAVCLEDFRRKEEVTTTPCNHMFHEECIVPWVKSHGQCPNCRSALFERTRRGGASSGNTV 220
           C VC ++F  K E    PC+H++H +CIVPW+  H  CP CR  L  R    G+SS    
Sbjct: 186 CPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR----GSSSSTQS 245

Query: 221 SIGRPASG 229
           S  R  +G
Sbjct: 246 SQNRSTNG 249

The following BLAST results are available for this feature:

BLAST of Sed0016413 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888228.1	4.9e-94	68.25	uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida]	[more]
XP_022989128.1	1.0e-91	64.18	E3 ubiquitin-protein ligase EL5 [Cucurbita maxima]	[more]
XP_008455285.1	3.9e-91	68.00	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo]	[more]
XP_023529368.1	6.7e-91	63.48	E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo]	[more]
XP_004136838.1	1.9e-90	66.91	E3 ubiquitin-protein ligase AIRP1 [Cucumis sativus] >KGN43640.1 hypothetical pro...	[more]

BLAST of Sed0016413 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y4L5	1.6e-10	54.17	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2	[more]
Q91YL2	1.6e-10	52.08	E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1	[more]
P0CH30	2.1e-10	40.28	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q6IRP0	3.5e-10	50.00	E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 ...	[more]
Q9D0C1	3.5e-10	43.48	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1	[more]

BLAST of Sed0016413 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JPC8	5.0e-92	64.18	E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4...	[more]
A0A1S3C054	1.9e-91	68.00	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A0A0K1V1	9.4e-91	66.91	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 P...	[more]
A0A5A7SLQ9	1.6e-90	67.77	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5D3C526	2.1e-90	67.77	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of Sed0016413 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58720.1	3.7e-23	38.96	RING/U-box superfamily protein	[more]
AT3G58720.2	3.7e-23	38.96	RING/U-box superfamily protein	[more]
AT2G44330.1	2.5e-11	48.08	RING/U-box superfamily protein	[more]
AT3G56580.2	7.3e-11	41.18	RING/U-box superfamily protein	[more]
AT3G56580.1	7.3e-11	41.18	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 90..130 e-value: 6.5E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 89..131 e-value: 4.1E-14 score: 52.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 90..131 score: 13.049892
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 75..146 e-value: 3.9E-21 score: 76.0
None	No IPR available	PANTHER	PTHR22765:SF328	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 47..180
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 47..180
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 90..131 e-value: 3.34551E-20 score: 77.3421
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 84..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0016413.1	Sed0016413.1	mRNA
Sed0016413.2	Sed0016413.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity