Sed0016274 (gene) Chayote v1

Overview
NameSed0016274
Typegene
OrganismSechium edule (Chayote v1)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationLG08: 36369116 .. 36370799 (+)
RNA-Seq ExpressionSed0016274
SyntenySed0016274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGAAGTATACATGGTTCGAGTTAGTTGTGAGTACCATTGCCAGGAGTCACCCGATATCACCCATTGCCAGGAGGCCAAAACCCCGGACCAACGGGGGATCGACTGCTGCCCACCAAAAGTCGAAGACATCATCGACTTCAACAGCACCCCACATCCGACGGGCCGCTCATTTGATGACCTGCGACCAAGTCACTCAATTTGAATTAGCCATTACGAAAATGAAAGAGTTGCACGAAGACGACCCACGTAGCTTTTGTCAACAAGCTAAGGTTCACTGTGCCTATTGTAATGACGGGTACAAACAGTTGGGGTTCCCGAAGGTAGATATGTTAGTCCATTGGAACGCCTTGTTCTTCCCGTTCCACCGCGCCTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTGGTCCACGACCCGACTTTCACCATACCCTTTTGGAATTGGGATTCAGAACCTGGCATGCAAATGCCATCAATGTACAACAACCCAAATTCATCACTCTACGATAAGCTCCGCGATGCGCGTCACAAGCCACCCACATTGCTTTACCTCAACTACGGCGGGGACGAAAACCCCCCTTCCGACCTCGTGTATTTTGGATGAATAAACAAATGAGCGTGAAGACACCCCAAGAGTTCCTTGGGAAGTTGCTCAAAGGCGGTGAGGAGGCTGTTAGGACTGGGGGCATGGGTTCCATCCAGAGCTCTCCCCACAACAACCTTCATGATTGGGTCGGCAGTCCAATATACCCGAACGAGGATATGGGAACTCTTTACGCGGCGGGTCGAGATCCATGGCAACGTTGATCGCATGTGGAGTCTTTGGCAAAAGATGGGGAGTGGACCAAGAGATTACACCTCTCCACATTGGTTGAACTCCTCGTTTGTGCTCTATGATGAGCAACAAAACGCTGTGCGCATCAAAGTTTCTGATTGTTTGGACAATGCTAAACTTGGGTACGTTTATGAAGATGTAGACATTCCGTGGCTCAAAACCAAGCAATTATCCCGGAATTTACACAAAAGGTATGCTTGTCATTGGAGTGGACGTAATTAGCATGAAGTTAATGAACCACGTAAATGGATGTGTTATTTGTTTCATGTTTTTCTTTAATTCTTGATCGTTCTTTCTAATAAATTTTGAAAATAGAAGTTTATTGAAGTTTATTTTGCAATATAAGTCTTTCAGCTTGCTCTTTTTTATAATTCCTAACAGTTTCCGTTTGTTCATAAAAGGTCACAAAAATCCAAAGAACCATCCAAACCACCTCCTGTCATCAGCTTCCCTCAGGTTCTGGATTCCAAGATAACCACGATGGTTCAAAGGCCGGACATGCCGAGGACCGGGAAGCAACTGAGGGAGGAAGAAGAGGTGTTGGTGATTGAAGACATCGAGTTCAATCCGACATATGGAGCGCATTTTAACGTGCTTGTTAATGTTATAGATGATGGGTCGGATGTGTGCCCGGGGAATACTGAGTTTGTGGGCACCTTCAAGACCATCCCGCACGGCCACACGCACCGAGCGAAAACCACGTTACGTTTTGTGATAAGTGAGCTTATTCAGGAGCTGGGGTTGGAGGGGAGTGAAGCTTTGGCTGTGAGGATTGACCCCAAGGTTGGAGGAGAGAATATCAGCATTGGAGGGATCAAGATTGAGCTACAGCCTTTGACTTGA

mRNA sequence

ATGAAAGAAGTATACATGGTTCGAGTTAGTTGTGAGTACCATTGCCAGGAGTCACCCGATATCACCCATTGCCAGGAGGCCAAAACCCCGGACCAACGGGGGATCGACTGCTGCCCACCAAAAGTCGAAGACATCATCGACTTCAACAGCACCCCACATCCGACGGGCCGCTCATTTGATGACCTGCGACCAAAGTTGCACGAAGACGACCCACGTAGCTTTTGTCAACAAGCTAAGGTTCACTGTGCCTATTGTAATGACGGGTACAAACAGTTGGGGTTCCCGAAGGTAGATATGTTAGTCCATTGGAACGCCTTGTTCTTCCCGTTCCACCGCGCCTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTGGTCCACGACCCGACTTTCACCATACCCTTTTGGAATTGGGATTCAGAACCTGGCATGCAAATGCCATCAATGTACAACAACCCAAATTCATCACTCTACGATAAGCTCCGCGATGCGCGTCACAAGCCACCCACATTGCTTTACCTCAACTACGGCGGGGACGAAAACCCCCCTTCCGACCTCGTACACCCCAAGAGTTCCTTGGGAAGTTGCTCAAAGGCGGTGAGGAGGCTGTTAGGACTGGGGGCATGGGTTCCATCCAGAGCTCTCCCCACAACAACCTTCATGATTGGGTCGGCAGTCCAATATACCCGAACGAGGATATGGGAACTCTTTACGCGGCGGGTCGAGATCCATGGCAACGTTGATCGCATGTGGAGTCTTTGGCAAAAGATGGGGAGTGGACCAAGAGATTACACCTCTCCACATTGGTTGAACTCCTCGTTTGTGCTCTATGATGAGCAACAAAACGCTGTGCGCATCAAAGTTTCTGATTGTTTGGACAATGCTAAACTTGGGTACGTTTATGAAGATGTAGACATTCCGTGGCTCAAAACCAAGCAATTATCCCGGAATTTACACAAAAGGTCACAAAAATCCAAAGAACCATCCAAACCACCTCCTGTCATCAGCTTCCCTCAGGTTCTGGATTCCAAGATAACCACGATGGTTCAAAGGCCGGACATGCCGAGGACCGGGAAGCAACTGAGGGAGGAAGAAGAGGTGTTGGTGATTGAAGACATCGAGTTCAATCCGACATATGGAGCGCATTTTAACGTGCTTGTTAATGTTATAGATGATGGGTCGGATGTGTGCCCGGGGAATACTGAGTTTGTGGGCACCTTCAAGACCATCCCGCACGGCCACACGCACCGAGCGAAAACCACGTTACGTTTTGTGATAAGTGAGCTTATTCAGGAGCTGGGGTTGGAGGGGAGTGAAGCTTTGGCTGTGAGGATTGACCCCAAGGTTGGAGGAGAGAATATCAGCATTGGAGGGATCAAGATTGAGCTACAGCCTTTGACTTGA

Coding sequence (CDS)

ATGAAAGAAGTATACATGGTTCGAGTTAGTTGTGAGTACCATTGCCAGGAGTCACCCGATATCACCCATTGCCAGGAGGCCAAAACCCCGGACCAACGGGGGATCGACTGCTGCCCACCAAAAGTCGAAGACATCATCGACTTCAACAGCACCCCACATCCGACGGGCCGCTCATTTGATGACCTGCGACCAAAGTTGCACGAAGACGACCCACGTAGCTTTTGTCAACAAGCTAAGGTTCACTGTGCCTATTGTAATGACGGGTACAAACAGTTGGGGTTCCCGAAGGTAGATATGTTAGTCCATTGGAACGCCTTGTTCTTCCCGTTCCACCGCGCCTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTGGTCCACGACCCGACTTTCACCATACCCTTTTGGAATTGGGATTCAGAACCTGGCATGCAAATGCCATCAATGTACAACAACCCAAATTCATCACTCTACGATAAGCTCCGCGATGCGCGTCACAAGCCACCCACATTGCTTTACCTCAACTACGGCGGGGACGAAAACCCCCCTTCCGACCTCGTACACCCCAAGAGTTCCTTGGGAAGTTGCTCAAAGGCGGTGAGGAGGCTGTTAGGACTGGGGGCATGGGTTCCATCCAGAGCTCTCCCCACAACAACCTTCATGATTGGGTCGGCAGTCCAATATACCCGAACGAGGATATGGGAACTCTTTACGCGGCGGGTCGAGATCCATGGCAACGTTGATCGCATGTGGAGTCTTTGGCAAAAGATGGGGAGTGGACCAAGAGATTACACCTCTCCACATTGGTTGAACTCCTCGTTTGTGCTCTATGATGAGCAACAAAACGCTGTGCGCATCAAAGTTTCTGATTGTTTGGACAATGCTAAACTTGGGTACGTTTATGAAGATGTAGACATTCCGTGGCTCAAAACCAAGCAATTATCCCGGAATTTACACAAAAGGTCACAAAAATCCAAAGAACCATCCAAACCACCTCCTGTCATCAGCTTCCCTCAGGTTCTGGATTCCAAGATAACCACGATGGTTCAAAGGCCGGACATGCCGAGGACCGGGAAGCAACTGAGGGAGGAAGAAGAGGTGTTGGTGATTGAAGACATCGAGTTCAATCCGACATATGGAGCGCATTTTAACGTGCTTGTTAATGTTATAGATGATGGGTCGGATGTGTGCCCGGGGAATACTGAGTTTGTGGGCACCTTCAAGACCATCCCGCACGGCCACACGCACCGAGCGAAAACCACGTTACGTTTTGTGATAAGTGAGCTTATTCAGGAGCTGGGGTTGGAGGGGAGTGAAGCTTTGGCTGTGAGGATTGACCCCAAGGTTGGAGGAGAGAATATCAGCATTGGAGGGATCAAGATTGAGCTACAGCCTTTGACTTGA

Protein sequence

MKEVYMVRVSCEYHCQESPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSFDDLRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGDENPPSDLVHPKSSLGSCSKAVRRLLGLGAWVPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSGPRDYTSPHWLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEPSKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVNVIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPKVGGENISIGGIKIELQPLT
Homology
BLAST of Sed0016274 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 485.3 bits (1248), Expect = 5.8e-133
Identity = 254/497 (51.11%), Postives = 318/497 (63.98%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPT----GRSFDDLRPK-------- 77
           +PD+ +C+ A       I CCPPK+ DIIDF   PH T     +    L PK        
Sbjct: 77  APDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKA 136

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 137 IHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 196

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD++ GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 197 RILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 256

Query: 198 ENPPSDLV-------HPK------------SSLGSCSKAVRRLLGLGA-----------W 257
           +NPP D+V       H +              L   S+      G+G            W
Sbjct: 257 DNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNW 316

Query: 258 VPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTSPH 317
           V     P     +   V Y+  R   LF      H NVDRMWS+WQ + +G PRD+ +P 
Sbjct: 317 VGD---PNAKNNMNMGVFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKPRDFNNPD 376

Query: 318 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEP 377
           WLNSSFV Y+E + AVRIKVSDCLD  +LGYVY+ VD+PWL TKQ       R +K  + 
Sbjct: 377 WLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQLKT 436

Query: 378 SKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVN 437
           ++ P VI FPQ L+S +TT V+RP   R  K+  E++EVLVIE IE +  Y  HFNVLVN
Sbjct: 437 TQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVN 496

Query: 438 VIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPK 466
           V+DDGSDVCPGNTEFVGTF T+ HGH HR KT+LR VI+E+++ELG+E  E++ VR+DPK
Sbjct: 497 VVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPK 556

BLAST of Sed0016274 vs. NCBI nr
Match: XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.4 bits (1243), Expect = 2.2e-132
Identity = 253/497 (50.91%), Postives = 315/497 (63.38%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSF----DDLRPK-------- 77
           +PD+ +C+ A     + I CCPPK+  IIDF   PH T          L PK        
Sbjct: 74  APDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKA 133

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 134 IHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 193

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD+  GMQMP +Y+NPNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 194 RILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYDKLRNPRHRPHKLLNLNYHGD 253

Query: 198 ENPPSDLV-------HPK------------SSLGSCSKAVRRLLGLGA-----------W 257
           +NP  D+V       H +              L   S       G+G            W
Sbjct: 254 DNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHEANEETGMGTIERSPHNNIHNW 313

Query: 258 VPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTSPH 317
           V     P     +     Y+  R   LF      H NVDRMWS+WQ + +G PRD+ +P 
Sbjct: 314 VGD---PNAKNHMNMGAFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKPRDFDNPD 373

Query: 318 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEP 377
           WLNSSFV Y+E + AVRIKVSDCLD  +LGYVY+ VD+PWL TKQ       R +K  + 
Sbjct: 374 WLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQLKA 433

Query: 378 SKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVN 437
           ++ P VI FPQ L+S +TT V+RP+  R  K+  E++EVLVIE IE +  Y  HFNVLVN
Sbjct: 434 TQAPQVIKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVN 493

Query: 438 VIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPK 466
           V+DDGSDVCPGNTEFVGTF T+PHGH HR KT+LR VI+E+++ELG+E  E++ VR+DPK
Sbjct: 494 VVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPK 553

BLAST of Sed0016274 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478.0 bits (1229), Expect = 9.2e-131
Identity = 249/497 (50.10%), Postives = 312/497 (62.78%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSF----DDLRPK-------- 77
           +PD+ +C+ A     + I CCPPK+ DIIDF   PH T          L PK        
Sbjct: 82  APDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKA 141

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 142 IHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 201

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD+  GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 202 RILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 261

Query: 198 ENPPSDLVHPK-------------------SSLGSCSKAVRRLLGLGA-----------W 257
           +NP  D+V                        L   S       G+G            W
Sbjct: 262 DNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIERSPHNNIHNW 321

Query: 258 VPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTSPH 317
           V     P     +     Y+  R   LF      H NVDRMWS+WQ + +G PRD+ +P 
Sbjct: 322 VGD---PNAKNHMNMGAFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKPRDFDNPD 381

Query: 318 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEP 377
           WLNSSFV Y+E + AVRIKVSDCLD  +LGYVY+ VD+PWL TKQ       R +K  + 
Sbjct: 382 WLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQLKT 441

Query: 378 SKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVN 437
           ++ P VI FPQ L+S +TT V+RP+     K+  E++EVLVIE IE +  +  HFNVLVN
Sbjct: 442 TQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVN 501

Query: 438 VIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPK 466
           V+DDGSDVCPGNTEFVGTF T+PHGH HR KT+LR VI+E+++ELG+E  E++ VR+DPK
Sbjct: 502 VVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPK 561

BLAST of Sed0016274 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 476.1 bits (1224), Expect = 3.5e-130
Identity = 248/497 (49.90%), Postives = 311/497 (62.58%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSF----DDLRPK-------- 77
           +PD+ +C+ A     + I CCPPK+  IIDF   PH T          L PK        
Sbjct: 77  APDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKA 136

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 137 IHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 196

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD+  GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 197 RILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 256

Query: 198 ENPPSDLVHPK-------------------SSLGSCSKAVRRLLGLGA-----------W 257
           +NP  D+V                        L   S       G+G            W
Sbjct: 257 DNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLLVASDEANEETGMGTIERSPHNNIHNW 316

Query: 258 VPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTSPH 317
           V     P     +     Y+  R   LF      H NVDRMWS+WQ + +G PRD+ +P 
Sbjct: 317 VGD---PNAKNHMNMGAFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKPRDFDNPD 376

Query: 318 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEP 377
           WLNSSFV Y+E + AVRIKV DCLD  +LGYVY+ VD+PWL TKQ       R +K  + 
Sbjct: 377 WLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQLKT 436

Query: 378 SKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVN 437
           ++ P VI FPQ L+S +TT V+RP+     K+  E++EVLVIE IE +  +  HFNVLVN
Sbjct: 437 TQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVN 496

Query: 438 VIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPK 466
           V+DDGSDVCPGNTEFVGTF T+PHGH HR KT+LR VI+E+++ELG+E  E++ VR+DPK
Sbjct: 497 VVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPK 556

BLAST of Sed0016274 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 475.7 bits (1223), Expect = 4.6e-130
Identity = 246/499 (49.30%), Postives = 314/499 (62.93%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSF----DDLRPK-------- 77
           +PD+ +C+ A       I CCPPK+ DIIDF   PH T          L PK        
Sbjct: 77  APDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKA 136

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 137 IHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 196

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD+  GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 197 RILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 256

Query: 198 ENPPSDLV--------------------------------HPKSSLGSCSKAVRRLLGLG 257
           +NP  D+V                                + ++ +G+  K+      + 
Sbjct: 257 DNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIEKSPHN--NIH 316

Query: 258 AWVPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTS 317
            WV     P     +     Y+  R   LF      H NVDRMWS+WQ + +G  RD+ +
Sbjct: 317 NWVGD---PNAKNHMNMGAFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKARDFDN 376

Query: 318 PHWLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSK 377
           P WLNSSFV Y+E + AVRIKV DCLD  +LGYVY+ VD+PWL TKQ       R +K  
Sbjct: 377 PDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQL 436

Query: 378 EPSKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVL 437
           + ++ P VI FPQ L+S +TT V+RP+     K+  E++EVLVIE IE +  +  HFNVL
Sbjct: 437 KTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVL 496

Query: 438 VNVIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRID 466
           VNV+DDGSDVCPGNTEFVGTF T+PHGH HR KT+LR VI+E+++ELG+E  E++ VR+D
Sbjct: 497 VNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLD 556

BLAST of Sed0016274 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.8e-90
Identity = 200/506 (39.53%), Postives = 270/506 (53.36%), Query Frame = 0

Query: 19  PDITHCQEAKTPDQR-GIDCCPPKVEDIIDF----------NSTPHPTGRSFDD------ 78
           PD++ C  A  P      +CCPP    IIDF              H   +++ D      
Sbjct: 95  PDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAM 154

Query: 79  -LRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFER 138
            L   L +DDPRSF QQA VHCAYC+  Y Q+GFP++++ +H + LFFPFHR YLYFFE+
Sbjct: 155 ELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEK 214

Query: 139 ILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGDE 198
           ILG L++DPTF +PFWNWDS  GM +P++Y +P S LYDKLR A H+PPTL+ L+Y G E
Sbjct: 215 ILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTE 274

Query: 199 NPPSDLVHPKSSLG-------SCSKAVRRLLG-----------------------LGAWV 258
           +  S      ++L        S SK  +   G                       +  W 
Sbjct: 275 DNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWT 334

Query: 259 PSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSGPRDYTSPHWL 318
                P    M G+     R  I+         H NVDRMWS+W+ +G    D T   WL
Sbjct: 335 GDNTQPNFEDM-GNFYSAGRDPIF------FAHHSNVDRMWSIWKTLGGKRTDLTDSDWL 394

Query: 319 NSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRN----LHKRSQK-- 378
           +S F+ Y+E    VR+KV DCL+   LGYVY+DVDIPWL +K   R     L K ++K  
Sbjct: 395 DSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLG 454

Query: 379 -----SKEPSKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLRE-EEEVLVIEDIEFNPT 438
                    SK      FP  L SKI+T+V+RP   +  K+ +E EEE+LVIE IEF+  
Sbjct: 455 VAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRD 514

Query: 439 YGAHFNVLVNVIDDGSDVCPGNTEFVGTFKTIPHGHTHRAK--TTLRFVISELIQELGLE 463
               F+V VN +DD     P  TEF G+F ++PH H H+ K  T LR  +++L++E+  E
Sbjct: 515 VAVKFDVYVNDVDD-LPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAE 574

BLAST of Sed0016274 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.0e-84
Identity = 192/502 (38.25%), Postives = 271/502 (53.98%), Query Frame = 0

Query: 17  ESPDITHCQEAKTPD-QRGIDCCPPKVEDIIDF----NSTPHPTG--------------R 76
           ++PDI+ C  A  PD     +CCPP    IIDF    + +P  T               +
Sbjct: 107 QAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYK 166

Query: 77  SFDDLRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYF 136
              +L+  L +DDPRSF QQA VHC YC   Y Q+G+  +++ VH + LF PFHR YLYF
Sbjct: 167 KAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYYLYF 226

Query: 137 FERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYG 196
            ERIL  L+ DPTF +P+W WD+  GM MP++Y +  SSLYD+ R+A+H PPT++ L+Y 
Sbjct: 227 NERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYD 286

Query: 197 GDEN--PPSDL----------------VHPKSSLG---SCSKAVRRLLGLGAWVPSRALP 256
           G E   P  +L                  PK  LG       A+    G     P   + 
Sbjct: 287 GTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRAGDAIDPGAGTLEHAPHNIVH 346

Query: 257 TTTFMIGSAVQ-----YTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSGPR-DYTSPHWL 316
             T +     +     YT  R    F      H NVDRMW++W+ +G   R D+T   WL
Sbjct: 347 KWTGLADKPSEDMGNFYTAGRDPIFFGH----HANVDRMWNIWKTIGGKNRKDFTDTDWL 406

Query: 317 NSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWL----KTKQLSRNLHKRSQKSK 376
           +++FV YDE +  V++KVSDC+D +KL Y Y+D+ IPWL    K K  +     +S  +K
Sbjct: 407 DATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKNTKAKAKTTTKSSKSGVAK 466

Query: 377 EPSKPPPVIS----FPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAH 436
               P   IS    FP+ L+S I   V RP   R+ K+  +EEEVL+I+ IE +      
Sbjct: 467 AAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENFVK 526

Query: 437 FNVLVNVIDDGSDVCPGNTEFVGTFKTIPHGHTH--RAKTTLRFVISELIQELGLEGSEA 463
           F+V +N  +D S   P N+EF G+F  +PH H    + KT LRF I+EL+++LG E  E+
Sbjct: 527 FDVYIN-DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKTNLRFAINELLEDLGAEDDES 586

BLAST of Sed0016274 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.0e-84
Identity = 191/513 (37.23%), Postives = 265/513 (51.66%), Query Frame = 0

Query: 19  PDITHC------QEAKTPDQRGIDCCPPKVEDIIDF----------NSTPHPTGRSF--- 78
           PD++ C          TP    I+CCPP    I DF              H     F   
Sbjct: 98  PDLSKCVPPSDLPSGTTPP--NINCCPPYSTKITDFKFPSNQPLRVRQAAHLVDNEFLEK 157

Query: 79  ----DDLRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYL 138
                +L   L  +DPR+F QQA +HCAYC+  Y Q+GFP + + VH + LFFPFHR YL
Sbjct: 158 YKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQVHGSWLFFPFHRWYL 217

Query: 139 YFFERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLN 198
           YF+ERILG L++DPTF +PFWN+D+  GMQ+P++Y +  S LYD+LR+A H+PPTL+ LN
Sbjct: 218 YFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLN 277

Query: 199 YG--GDENPPSDLVHPKSSL-----GSCSKAVRRLLGLGAWVPSRALPTTTFMIGSA--V 258
           +   G +   ++L+    S+      S  K  R  LG     P RA        GS   V
Sbjct: 278 FCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLG----NPYRAGDAEPQGAGSIENV 337

Query: 259 QYTRTRIWELFTRRVEI------------------HGNVDRMWSLWQKMGSGPRDYTSPH 318
            +     W     +  I                  H NVDR+W +W+ +G    D+T   
Sbjct: 338 PHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDKD 397

Query: 319 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKR------- 378
           WL S F+ YDE +N VR+ V D LD  KLGY Y+DV IPW K K + R    +       
Sbjct: 398 WLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRTKVQKLVEVEV 457

Query: 379 --SQKSKEPS-------KPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIED 438
                 K P+        P   ++FP VL++K++ +V+RP   R+ K+  EEEEVLVIE 
Sbjct: 458 NDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEEEVLVIEG 517

Query: 439 IEFNPTYGAHFNVLVNVIDDGSDVCPGNTEFVGTFKTIPH-GHTHRAK---TTLRFVISE 462
           IEF       F+V +N  D+   V  GNTEF G+F  IPH  H H+ K   T+LR  I++
Sbjct: 518 IEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAHGHKNKKIITSLRLGITD 577

BLAST of Sed0016274 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.1e-83
Identity = 182/479 (38.00%), Postives = 247/479 (51.57%), Query Frame = 0

Query: 37  CCPPKVEDIIDFNSTPHPTGRSFDDLRPKLH---------------------EDDPRSFC 96
           CCPP V+D IDF    +P+      +RP  H                     ++DPRSF 
Sbjct: 105 CCPPDVKDPIDFQMPSNPSRLR---IRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFY 164

Query: 97  QQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFERILGHLVHDPTFTIPF 156
           QQA +HCAYCN  Y Q+GFP V++ VH + LF PFHR YLYF+ERILG L+ DPTF IPF
Sbjct: 165 QQANIHCAYCNGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPF 224

Query: 157 WNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGDENPPSDLVHPKSSLGS 216
           WNWD+  GM MP M+ +PNS LYD  R+  H P T++ L++   E P  +     ++L  
Sbjct: 225 WNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSI 284

Query: 217 CSKAVRRLLGLGAWVPSRALPTTTFMIGS------AVQYTRTRIWELFTRRVEI------ 276
             K V R      +   R L   +F   S         +    +W    R+         
Sbjct: 285 MYKQVVRAKKARLF-HGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNF 344

Query: 277 ------------HGNVDRMWSLWQKMGSGPRDYTSPHWLNSSFVLYDEQQNAVRIKVSDC 336
                       H NVDRMW +W K   G +DY    WLN+ F+L+DE    VR+ V D 
Sbjct: 345 YSAGRDPLFYAHHANVDRMWYIW-KNSLGRKDYKKKDWLNAGFLLFDENAQPVRVYVRDA 404

Query: 337 LDNAKLGYVYEDVDIPWLKTKQLSR--NLHKRSQKSKEPSKPPPVISFPQVLDSKITTMV 396
           LD  KLGY Y++VDIPW+ +K   R  N      +SK  +    +  FP  LDS ++  V
Sbjct: 405 LDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEV 464

Query: 397 QRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVNVIDDGSDVCPGNTEFVGTFKT 456
           +RP   R+  +  +EEEVLVIE I+  P +   F+V +N  D+        TEF G+F  
Sbjct: 465 KRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSAA-DQTEFAGSFVN 524

Query: 457 IPHGHTH------RAKTTLRFVISELIQELGLEGSEALAVRIDPKVGGENISIGGIKIE 463
           +PH H H      R  T L   ISE++ EL ++G +++ V + P+VG   I+IGG KIE
Sbjct: 525 VPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of Sed0016274 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-81
Identity = 195/499 (39.08%), Postives = 262/499 (52.51%), Query Frame = 0

Query: 18  SPDITHC-QEAKTPDQRGIDCCPPKVEDIIDFNSTPHPT-------GRSFDD-------- 77
           +PD+T C Q    P    I+CCPP    IIDF   P  T           DD        
Sbjct: 65  APDVTKCGQPDLPPGTAPINCCPPIPAKIIDFELPPPSTTMRVRRAAHLVDDAYIAKFKK 124

Query: 78  ---LRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFF 137
              L   L EDDPRSF QQA VHCAYC   Y Q GF  + + +H + LFFPFHR Y+YFF
Sbjct: 125 AVELMRALPEDDPRSFKQQANVHCAYCAGAYNQAGFTNLKLQIHRSWLFFPFHRYYIYFF 184

Query: 138 ERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGG 197
           ERILG L++D TF +PFWN+DS  GM +PSM+ + NSSLYD LRD+ H+PPT++ LNY  
Sbjct: 185 ERILGKLINDTTFALPFWNYDSPGGMTIPSMFIDTNSSLYDSLRDSNHQPPTIVDLNYAF 244

Query: 198 DENPPSDLVHPKSSLGSCSKAVRRLLGLGAWVPS-------RALPTTTFMIGS--AVQYT 257
            ++   +   P+  +    K V R +   A  P        R        +GS   V + 
Sbjct: 245 SDS--DNTTTPEEQMIINLKIVYRQMVSSAKTPQLFFGRPYRRGDQEFPGVGSIELVPHG 304

Query: 258 RTRIWE--------------LFTRRVEI----HGNVDRMWSLWQKMGSGPR--DYTSPHW 317
              +W                 T R  I    H NVDRMWS+W+ +G GPR  D T P +
Sbjct: 305 MIHLWTGSENTPYGENMGAFYSTARDPIFFAHHSNVDRMWSIWKTLG-GPRRTDLTDPDF 364

Query: 318 LNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEPS 377
           L++SFV YDE    VR+KV DCLD  KLGYVY+DV+IPWL T+   +      +K    +
Sbjct: 365 LDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQDVEIPWLNTRPTPKVSPSLLKKFHRTN 424

Query: 378 KPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYG-AHFNVLVN 437
              P   FP +LD  +  +V RP   R+ K+  E EE+LVIE IE    +G   F+V +N
Sbjct: 425 TANPRQVFPAILDRVLKVIVTRPKKTRSRKEKDELEEILVIEGIELERDHGHVKFDVYIN 484

Query: 438 V-IDDGSDVCPGNTEFVGTFKTIPHG--HTHRAKT-TLRFVISELIQELGLEGSEALAVR 464
              DD + + P N EF G+F ++ H      R KT  L   I +++++L  +  + + V 
Sbjct: 485 ADEDDLAVISPENAEFAGSFVSLWHKPIKGKRTKTQLLTLSICDILEDLDADEDDYVLVT 544

BLAST of Sed0016274 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.8e-133
Identity = 254/497 (51.11%), Postives = 318/497 (63.98%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPT----GRSFDDLRPK-------- 77
           +PD+ +C+ A       I CCPPK+ DIIDF   PH T     +    L PK        
Sbjct: 77  APDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKA 136

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 137 IHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 196

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD++ GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 197 RILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 256

Query: 198 ENPPSDLV-------HPK------------SSLGSCSKAVRRLLGLGA-----------W 257
           +NPP D+V       H +              L   S+      G+G            W
Sbjct: 257 DNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNW 316

Query: 258 VPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTSPH 317
           V     P     +   V Y+  R   LF      H NVDRMWS+WQ + +G PRD+ +P 
Sbjct: 317 VGD---PNAKNNMNMGVFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKPRDFNNPD 376

Query: 318 WLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSKEP 377
           WLNSSFV Y+E + AVRIKVSDCLD  +LGYVY+ VD+PWL TKQ       R +K  + 
Sbjct: 377 WLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQLKT 436

Query: 378 SKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVN 437
           ++ P VI FPQ L+S +TT V+RP   R  K+  E++EVLVIE IE +  Y  HFNVLVN
Sbjct: 437 TQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVN 496

Query: 438 VIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPK 466
           V+DDGSDVCPGNTEFVGTF T+ HGH HR KT+LR VI+E+++ELG+E  E++ VR+DPK
Sbjct: 497 VVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPK 556

BLAST of Sed0016274 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 2.2e-130
Identity = 246/499 (49.30%), Postives = 314/499 (62.93%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDFNSTPHPTGRSF----DDLRPK-------- 77
           +PD+ +C+ A       I CCPPK+ DIIDF   PH T          L PK        
Sbjct: 77  APDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKA 136

Query: 78  ------LHEDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFE 137
                 L  +DPR+F +QA VHCAYC+  YKQLGFP  +M VHWNALFFPFHRAYLYFFE
Sbjct: 137 IHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFE 196

Query: 138 RILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYGGD 197
           RILGHL+++P F IPFWNWD+  GMQMP +Y++PNS LYDKLR+ RH+P  LL LNY GD
Sbjct: 197 RILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGD 256

Query: 198 ENPPSDLV--------------------------------HPKSSLGSCSKAVRRLLGLG 257
           +NP  D+V                                + ++ +G+  K+      + 
Sbjct: 257 DNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIEKSPHN--NIH 316

Query: 258 AWVPSRALPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSG-PRDYTS 317
            WV     P     +     Y+  R   LF      H NVDRMWS+WQ + +G  RD+ +
Sbjct: 317 NWVGD---PNAKNHMNMGAFYSAARD-PLFYGH---HANVDRMWSIWQGLKNGKARDFDN 376

Query: 318 PHWLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKSK 377
           P WLNSSFV Y+E + AVRIKV DCLD  +LGYVY+ VD+PWL TKQ       R +K  
Sbjct: 377 PDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQT-----PRLKKQL 436

Query: 378 EPSKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVL 437
           + ++ P VI FPQ L+S +TT V+RP+     K+  E++EVLVIE IE +  +  HFNVL
Sbjct: 437 KTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVL 496

Query: 438 VNVIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRID 466
           VNV+DDGSDVCPGNTEFVGTF T+PHGH HR KT+LR VI+E+++ELG+E  E++ VR+D
Sbjct: 497 VNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLD 556

BLAST of Sed0016274 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 7.4e-118
Identity = 241/494 (48.79%), Postives = 305/494 (61.74%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGIDCCPPKVEDIIDF------NSTPHPTGRSF-DDLRPKLHE-- 77
           SPD+ HC+         +DCCPP + DI DF      N    P       +   KL E  
Sbjct: 88  SPDVIHCEPITLEADEKVDCCPPSILDITDFVPPAVENLRVRPAAHLITQEQLAKLEEAV 147

Query: 78  --------DDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAYLYFFER 137
                   DDPR+F QQA+VHCAYCND YKQ GFP++D  +HWN LFFPFHR YLYF ER
Sbjct: 148 TKMKALDPDDPRNFYQQARVHCAYCNDAYKQDGFPELDFDIHWNWLFFPFHRCYLYFHER 207

Query: 138 ILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNN--PNSSLYDKLRDARHKPPTLLYLNY-G 197
           ILG L++DP F IPFWNWD+ PGMQMP++Y N   NS LYD+LRD  HKPP LL LNY G
Sbjct: 208 ILGSLINDPDFAIPFWNWDAIPGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDG 267

Query: 198 GDENPPSD-------------LVHPKSSLGSCSKAVRR-----LLGLGAWVPSRALPTTT 257
             E+P  D             +++ K++ G   K +R        G+G+ +   A     
Sbjct: 268 ATEDPSYDVLFCNPRLMYRQMVINSKTAEGFHGKPLRAGQEAYRTGMGS-IELSAHNNVH 327

Query: 258 FMIGSAVQYTRTRIWELFTRRVE-----IHGNVDRMWSLWQKMGSG-PRDYTSPHWLNSS 317
              G   Q  R  +   +    +      H NVDRMW++WQ +  G  RD+T P WLN+S
Sbjct: 328 TWSGDPQQKYREDMGHFYAAARDPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNAS 387

Query: 318 FVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQ-KSKEPSKPP 377
           FV YDE +NA+RIKV DCLDN KLGY Y+ V+IPWL TK + R   K SQ KS +  + P
Sbjct: 388 FVFYDENKNAIRIKVQDCLDNEKLGYKYQAVEIPWLHTKPIPR--QKTSQLKSSKAQETP 447

Query: 378 PVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVNVIDD 437
           P+    +VL+SK+T  V+RP   R+  Q   E+EVLVIE +E + T   HFNVLVNV+DD
Sbjct: 448 PI----EVLNSKMTKEVKRPQRSRSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDD 507

Query: 438 GSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPKVGGE 467
           G+DV PGNTEF G+FK+IP GH HRA T+L  VI+ELI+ELG +  E + V IDPK+GG+
Sbjct: 508 GTDVSPGNTEFAGSFKSIPLGHKHRANTSLSLVITELIEELGYDDDETVFVTIDPKLGGD 567

BLAST of Sed0016274 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 6.0e-112
Identity = 233/493 (47.26%), Postives = 297/493 (60.24%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRGI-DCCPPKVEDIIDF------------------NSTPHPTGRS 77
           +PDI +C  AKTP   GI DCCPP++  II F                  N T      S
Sbjct: 78  TPDIFNCHMAKTPG--GIKDCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELES 137

Query: 78  FDDLRPKLHEDDPRSFCQQAKVHCAYCNDGYKQLG-FPKVDMLVHWNALFFPFHRAYLYF 137
                 +L E DP SF  QAK+HCAYC+  Y QLG   +    VH NALFFPFHRAYLYF
Sbjct: 138 GIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYF 197

Query: 138 FERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYLNYG 197
           FERIL H + +P F IPFWNWDS PGM MP  YN P+SSLYD LRD RH P  L+YLNY 
Sbjct: 198 FERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYN 257

Query: 198 G-DENPPSDLV--------------HPKSSLGSCSKAVRRL----LGLGAWVPSRA---- 257
           G  E+   D+V               P+S LG   KA        +G     P       
Sbjct: 258 GKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDW 317

Query: 258 LPTTTFMIGSAVQYTRTRIWELFTRRVEIHGNVDRMWSLWQKMGSGPRDYTSPHWLNSSF 317
           L T T        +    +  LF      H N+DR+W++W+ +   P+D+  P WLNSSF
Sbjct: 318 LGTKTPHFEDMGSFYSAALDPLFYAH---HANIDRLWNIWKTLDGNPKDHIDPDWLNSSF 377

Query: 318 VLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQL-SRNLHKRSQKSKEPSKPPP 377
           V YDE +NAV IKVSDCLD  KLGYVY++V +PWL   ++ + N   R+++S+     PP
Sbjct: 378 VFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIRTEESE-----PP 437

Query: 378 VISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNVLVNVIDDG 437
           +++FPQ+L+S ITTMV RP+  R+G+   E +EVLV+ED+E++PT   HF+VLVNV+D  
Sbjct: 438 IVNFPQILNSDITTMVDRPEEDRSGE---EVDEVLVLEDVEYDPTTRVHFDVLVNVVDKA 497

Query: 438 SDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRIDPKVGGEN 467
            D C    EFVG+FK IPHG  H  KT+LRFVI+E+IQ LGLE  + LAV+I+P VGGE 
Sbjct: 498 QDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEG 557

BLAST of Sed0016274 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 7.9e-112
Identity = 233/502 (46.41%), Postives = 303/502 (60.36%), Query Frame = 0

Query: 18  SPDITHCQEAKTPDQRG--IDCCPPKVEDIIDFNSTPHPTGRSFDDLRPKLH-------- 77
           SPD+ +C+  K P   G  +DCCPPK+++II+F     PT R    +RP +H        
Sbjct: 82  SPDVHNCKPVK-PSAVGKKMDCCPPKIKEIIEFTPPVVPTPR----VRPAVHLMTPEQIA 141

Query: 78  -------------EDDPRSFCQQAKVHCAYCNDGYKQLGFPKVDMLVHWNALFFPFHRAY 137
                        EDDPRSF  QAKVHCAYC+D YKQ+    +D  VH NALFFPFHRAY
Sbjct: 142 DLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMD-STMDFQVHENALFFPFHRAY 201

Query: 138 LYFFERILGHLVHDPTFTIPFWNWDSEPGMQMPSMYNNPNSSLYDKLRDARHKPPTLLYL 197
           LYFFERIL HL+  P F IPFWNWD+ PGM MP +Y  P+SSLYD+ RD  H+PP LL L
Sbjct: 202 LYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSLYDENRDKCHQPPKLLNL 261

Query: 198 NYGGDENPPSDLV-------HPKSSLGSCSKAVRRLL--GLGAWVPSRALPTTTFMIGSA 257
           NYGG E    ++V       + + S+ +  + + +++  G G    S ++ T+       
Sbjct: 262 NYGGKEVKDPEIVDFNLRWMNDQMSVETAKEFLGKVIKRGNGPENGSGSIETS------- 321

Query: 258 VQYTRTRIWELFTRRVEIHG--------------------NVDRMWSLWQKMGSGPRDYT 317
             +    IW +      IHG                    NVDRMW++WQKM   PRD+T
Sbjct: 322 -PHNNIHIW-VGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNIWQKMKDRPRDHT 381

Query: 318 SPHWLNSSFVLYDEQQNAVRIKVSDCLDNAKLGYVYEDVDIPWLKTKQLSRNLHKRSQKS 377
              WLNSSFVLYDE + AVRIKVS CLD+  LGYVY+DV IPW  T  + R   +RS   
Sbjct: 382 DMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPPIRRPKQERSL-- 441

Query: 378 KEPSKPPPVISFPQVLDSKITTMVQRPDMPRTGKQLREEEEVLVIEDIEFNPTYGAHFNV 437
                 PP I FPQ+L S ITTMV RP++    +++  + EVLV+ED+E++PT   HF+V
Sbjct: 442 ---GNLPPTIDFPQILSSDITTMVDRPEVASPEERI-NKNEVLVLEDVEYDPTLPLHFDV 501

Query: 438 LVNVIDDGSDVCPGNTEFVGTFKTIPHGHTHRAKTTLRFVISELIQELGLEGSEALAVRI 467
           L+NV D+G DV  G+ EFVGTF  +PHG T   KT+L FVI++ IQ+LGLEG + +AV+I
Sbjct: 502 LLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGDKKVAVKI 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022994369.15.8e-13351.11polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
XP_023542623.12.2e-13250.91polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7012418.19.2e-13150.10hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6573251.13.5e-13049.90hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022954613.14.6e-13049.30polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P433092.8e-9039.53Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
P433111.0e-8438.25Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q062153.0e-8437.23Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
Q6UIL31.1e-8338.00(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
Q9FRX61.1e-8139.08Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K2N92.8e-13351.11polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114901... [more]
A0A6J1GRK52.2e-13049.30polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A172DC017.4e-11848.79Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1[more]
A0A0A0LUI16.0e-11247.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1[more]
A0A5A7UTU07.9e-11246.41Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 102..119
score: 51.36
coord: 133..138
score: 57.82
coord: 236..254
score: 33.08
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 71..153
e-value: 1.6E-15
score: 58.0
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 102..119
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 337..463
e-value: 5.8E-33
score: 113.8
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 243..310
e-value: 7.2E-20
score: 73.7
coord: 16..229
e-value: 2.6E-41
score: 144.2
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 26..309
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 263..312
e-value: 5.5E-21
score: 74.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 317..336
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 243..464
coord: 65..179
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 243..464
coord: 65..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0016274.1Sed0016274.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0016491 oxidoreductase activity