Sed0016060 (gene) Chayote v1

Overview
NameSed0016060
Typegene
OrganismSechium edule (Chayote v1)
DescriptionNon-specific serine/threonine protein kinase
LocationLG08: 26904542 .. 26907672 (-)
RNA-Seq ExpressionSed0016060
SyntenySed0016060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAATAAGAAATTTACTTTAAACACTCTGAAAAACAGGCCGTCACTTTCATTCAACACCTTCTTTTTTTTTTCTGCAAACGAGAGATGTGCTTTCCAAATACTTCTCTCCCCAAAATCTGAGGAATCAAGAATTTTCAGCGCCCAAAAACGCTGCGCAAATCCGATCGCTCCAATGGAAGAAACCGATTCATCCTCTCATCTTCATCTTCTTCGACTTCCAGATTTCTCTCACTATGTTGAAATCGATCCCACTGGCCGATATGGAAGAGTATGATCCTACCAACTCGTTTTCTCTTTTGTGTTCTTCCGATTTGATTCTCATTGTTTCGTGTTCTTTGTTGCAGTACAATGAAATCCTCGGCAAAGGTGCTTCCAAGACAGTGTATGACGAATCTCGCATCTCCTTTGTGTTTTTTGCATTTTTGTGATTACCGTCGATTTTCGTGTTTTCGGCTGCATTTTTCGTTTGTTTTGTGAATTTTTTTTATCTCTTCTGCGTGTTTGATTTTTTGTTGATTTGATTCTGTGTTTTGGAAAATGGGCGTTGGATTTAGGTTTTTGTTTGTGGTTCAGTTACAGGGCGTTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTGAAAACATTGAAGCATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCGCATCGGAACATTAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTAAGAATGCTTTTCAATTACATTCTTCACTTTATATGCTACTAATTCTCTCACATTTCTGCTCAATTTCTGGTTGGTTCTGTTCTGCCAGATTTAGGCTTAAGCATAGGAGAGTTAACATTAGAGCCGTGAAGCGATGGTGCAGACAAATCTTGAGGGGTCTTCTCTATTTGCATAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGATAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTCGCTGCTATCCTTCGGAAATCGCACGCTGATCATTGCGTCGGTATGTTTATATTCTTCAAAACCTGTGAAATTTTGGCAATGGATTTCATTTCTCTGTAGATGGTGGTACCAATTGTTTTACTGTTTCATTCTTGGCTTTCTGATTCTTAATACCCTTCCAATCATATCTTTTCCTTGAATTGCTGGTTAGTCTTGTGAGAGAAGTAAGTCTTTTTGTTAGATGCTTATTATTGTTGTTATTGCATTACAGGGACACCTGAATTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCTTATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGTAAGATCTCCCTCTCTCAATGGGCACTATAATTTTCTTTAATCTTCTATATGAGGGGAGAAAAGGTTGATTCCTTAAGATGCTCTCTTACTTGTAGGGAAAAAAACCAGAATCCTTGTATAAAGTGAAGGATCTGAGCATGCGGCAATTTGTTGAGAAATGTTTGGCACCGGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGACTGTGAAAGCAAGTCGAAGCTTACAGAATTTCGGGGAGAACTTGAAGGTATTGCTTCTACTGCAGCACACCCTTTCCTTGAACGAGAAAGAAGCTTCAGATCAATAACTTACAGTTTGGAGGCCTCAGACGAATGGAGGTGTCGTACAGTTCAGACTGAACCCGATGGAATCGAACTATTTGAGAACGATGAGGATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGGGAAGATGGCAGCATTGTTATAAGACTTAGGATTGCAGATAAAGAAGGTAGTATCATATTTATTTACCTCCTTTTGCCATTGGTCATGTAATGATTGTTTTCTATTAATAGTTAACGAGATGTTCACTCAAACAATATTGTGTAAGTGCAGGTCAGATTCGAAACATATATTTCCCGTTTGACACAGAGAATGATACAGCATTTACCGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGACCAAAATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTTCCTGAGTGGAAGCCAGGGCCTGGCATAGATGAAACTCCTCGCATCTCTTATGATGGTGGCTGCCAAAGCTACGACTCTTCTAACCAACCATTCGATGATAGTTCGAAAGGAAATGAGCAAAATGTAGCAAAACTCTATCAGATTTTAAACTTAAGCACAGTTGAAAGGGCTTCAGTGCACAGACATTTCGACCAGGACTTCCTTAAACCAGATCATCCTCCGCAGCTTGTTGTAGAGAACCCCCCAAAAGTTTCAAGCCAACATTATCAGCCAAACTCCATCCTCCATGAAAACCAGGCTCGTAGTTCTCGAAGTTTTAGGCAAAGCCAATTTGATCACAATTACAAGAAAACAGGTCAGTCAATCACTGTTGAGGATAAAAAAGACAAAGTAATAGATAGCAGAGCCAACGTCATTGCAAGAACTTCACTCAGACGCTGGACGGGTTCCCGATCGCTGTCTACGGTTCGGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGACATTATTTAGAGAGATGAGTTGAAACTTCTCTGCAGCCTTTTAGATTGCAACAAACAAAAGAGGTTGTAGTTGGTATGAGTAATGTGATTATAGCCGCCCCATTCGGATGCTCAAAGGGCCCGAAATTGTGAGGCGTTTGTTTTCAGGGGTTAAGATTGCAGGGGTACATTGCCTCCAAACACTCTACTAGAGCAACTCTCTATCGGTTGTTGCCGTTTTTGTGTTGTACAGAAATATGTAAGTTACTCTCAGCACTTCTGAATTTCTTACACTCACTTTATATTTTCAAGGGTTAAGATGACAGCCATACTTGTTTTTTTTTTCCTTGATCATTGTTTTGG

mRNA sequence

AAGAAATAAGAAATTTACTTTAAACACTCTGAAAAACAGGCCGTCACTTTCATTCAACACCTTCTTTTTTTTTTCTGCAAACGAGAGATGTGCTTTCCAAATACTTCTCTCCCCAAAATCTGAGGAATCAAGAATTTTCAGCGCCCAAAAACGCTGCGCAAATCCGATCGCTCCAATGGAAGAAACCGATTCATCCTCTCATCTTCATCTTCTTCGACTTCCAGATTTCTCTCACTATGTTGAAATCGATCCCACTGGCCGATATGGAAGATACAATGAAATCCTCGGCAAAGGTGCTTCCAAGACAGTTTACAGGGCGTTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTGAAAACATTGAAGCATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCGCATCGGAACATTAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAATTTAGGCTTAAGCATAGGAGAGTTAACATTAGAGCCGTGAAGCGATGGTGCAGACAAATCTTGAGGGGTCTTCTCTATTTGCATAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGATAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTCGCTGCTATCCTTCGGAAATCGCACGCTGATCATTGCGTCGGGACACCTGAATTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCTTATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGAAAAAAACCAGAATCCTTGTATAAAGTGAAGGATCTGAGCATGCGGCAATTTGTTGAGAAATGTTTGGCACCGGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGACTGTGAAAGCAAGTCGAAGCTTACAGAATTTCGGGGAGAACTTGAAGGTATTGCTTCTACTGCAGCACACCCTTTCCTTGAACGAGAAAGAAGCTTCAGATCAATAACTTACAGTTTGGAGGCCTCAGACGAATGGAGGTGTCGTACAGTTCAGACTGAACCCGATGGAATCGAACTATTTGAGAACGATGAGGATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGGGAAGATGGCAGCATTGTTATAAGACTTAGGATTGCAGATAAAGAAGGTCAGATTCGAAACATATATTTCCCGTTTGACACAGAGAATGATACAGCATTTACCGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGACCAAAATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTTCCTGAGTGGAAGCCAGGGCCTGGCATAGATGAAACTCCTCGCATCTCTTATGATGGTGGCTGCCAAAGCTACGACTCTTCTAACCAACCATTCGATGATAGTTCGAAAGGAAATGAGCAAAATGTAGCAAAACTCTATCAGATTTTAAACTTAAGCACAGTTGAAAGGGCTTCAGTGCACAGACATTTCGACCAGGACTTCCTTAAACCAGATCATCCTCCGCAGCTTGTTGTAGAGAACCCCCCAAAAGTTTCAAGCCAACATTATCAGCCAAACTCCATCCTCCATGAAAACCAGGCTCGTAGTTCTCGAAGTTTTAGGCAAAGCCAATTTGATCACAATTACAAGAAAACAGGTCAGTCAATCACTGTTGAGGATAAAAAAGACAAAGTAATAGATAGCAGAGCCAACGTCATTGCAAGAACTTCACTCAGACGCTGGACGGGTTCCCGATCGCTGTCTACGGTTCGGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGACATTATTTAGAGAGATGAGTTGAAACTTCTCTGCAGCCTTTTAGATTGCAACAAACAAAAGAGGTTGTAGTTGGTATGAGTAATGTGATTATAGCCGCCCCATTCGGATGCTCAAAGGGCCCGAAATTGTGAGGCGTTTGTTTTCAGGGGTTAAGATTGCAGGGGTACATTGCCTCCAAACACTCTACTAGAGCAACTCTCTATCGGTTGTTGCCGTTTTTGTGTTGTACAGAAATATGTAAGTTACTCTCAGCACTTCTGAATTTCTTACACTCACTTTATATTTTCAAGGGTTAAGATGACAGCCATACTTGTTTTTTTTTTCCTTGATCATTGTTTTGG

Coding sequence (CDS)

ATGGAAGAAACCGATTCATCCTCTCATCTTCATCTTCTTCGACTTCCAGATTTCTCTCACTATGTTGAAATCGATCCCACTGGCCGATATGGAAGATACAATGAAATCCTCGGCAAAGGTGCTTCCAAGACAGTTTACAGGGCGTTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTGAAAACATTGAAGCATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCGCATCGGAACATTAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAATTTAGGCTTAAGCATAGGAGAGTTAACATTAGAGCCGTGAAGCGATGGTGCAGACAAATCTTGAGGGGTCTTCTCTATTTGCATAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGATAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTCGCTGCTATCCTTCGGAAATCGCACGCTGATCATTGCGTCGGGACACCTGAATTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCTTATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGAAAAAAACCAGAATCCTTGTATAAAGTGAAGGATCTGAGCATGCGGCAATTTGTTGAGAAATGTTTGGCACCGGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGACTGTGAAAGCAAGTCGAAGCTTACAGAATTTCGGGGAGAACTTGAAGGTATTGCTTCTACTGCAGCACACCCTTTCCTTGAACGAGAAAGAAGCTTCAGATCAATAACTTACAGTTTGGAGGCCTCAGACGAATGGAGGTGTCGTACAGTTCAGACTGAACCCGATGGAATCGAACTATTTGAGAACGATGAGGATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGGGAAGATGGCAGCATTGTTATAAGACTTAGGATTGCAGATAAAGAAGGTCAGATTCGAAACATATATTTCCCGTTTGACACAGAGAATGATACAGCATTTACCGTTGCCACCGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGACCAAAATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTTCCTGAGTGGAAGCCAGGGCCTGGCATAGATGAAACTCCTCGCATCTCTTATGATGGTGGCTGCCAAAGCTACGACTCTTCTAACCAACCATTCGATGATAGTTCGAAAGGAAATGAGCAAAATGTAGCAAAACTCTATCAGATTTTAAACTTAAGCACAGTTGAAAGGGCTTCAGTGCACAGACATTTCGACCAGGACTTCCTTAAACCAGATCATCCTCCGCAGCTTGTTGTAGAGAACCCCCCAAAAGTTTCAAGCCAACATTATCAGCCAAACTCCATCCTCCATGAAAACCAGGCTCGTAGTTCTCGAAGTTTTAGGCAAAGCCAATTTGATCACAATTACAAGAAAACAGGTCAGTCAATCACTGTTGAGGATAAAAAAGACAAAGTAATAGATAGCAGAGCCAACGTCATTGCAAGAACTTCACTCAGACGCTGGACGGGTTCCCGATCGCTGTCTACGGTTCGGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGA

Protein sequence

MEETDSSSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQLENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVAKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCEDKFASQIHWEIRWLWN
Homology
BLAST of Sed0016060 vs. NCBI nr
Match: XP_023001906.1 (probable serine/threonine-protein kinase WNK9 [Cucurbita maxima])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.8e-297
Identity = 522/615 (84.88%), Postives = 549/615 (89.27%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL GIA T AHPFLER+ SF SI Y LEASDEWRCR+VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L EN A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. NCBI nr
Match: KAG6588353.1 (putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022205.1 putative serine/threonine-protein kinase WNK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.8e-297
Identity = 521/615 (84.72%), Postives = 550/615 (89.43%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQI+DCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQINDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL+GIA T AHPFLER+ SF SI Y LEASDEWRCR+VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYHLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L E+ A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. NCBI nr
Match: XP_022933886.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1027.7 bits (2656), Expect = 4.1e-296
Identity = 519/615 (84.39%), Postives = 548/615 (89.11%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL+GIA T AHPFLER+ SF SI Y LEASDEWRC +VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L E+ A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. NCBI nr
Match: XP_023529957.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1027.3 bits (2655), Expect = 5.3e-296
Identity = 520/615 (84.55%), Postives = 548/615 (89.11%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL+ IA T AHPFLER+ SF SI Y LEASDEWRC +VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELDCIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L E+ A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. NCBI nr
Match: XP_038880702.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.3e-291
Identity = 513/617 (83.14%), Postives = 546/617 (88.49%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           M++TDS S    L++LPDFS YVEIDPTGRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA++NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQFRLKHRRVNIRAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP +LYKVKD SMRQFVEKCLAPVSCRLSARELLSDPFLQ+DD ESKSK+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +  RGEL+  AST AHPFLE+E+ F SI+YS E SDEWRC TVQ E DGIELFE+D++D 
Sbjct: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVP+WKPGPGIDETPRISYDGG +SY+ SNQP D+S   N+ N 
Sbjct: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQD--FLKPDHPPQLVVENPPKVSSQHYQPNSILHENQA 540
           AKLYQILN S   RA V  HF+Q    LK D P Q VVE+ PKVSSQH Q NS LHENQA
Sbjct: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540

Query: 541 RSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYC 600
            SS SFRQ     NYKKT QS+TV   K+KV D++A VI RTSLR   GSRSLSTV SYC
Sbjct: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 617

BLAST of Sed0016060 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 638.3 bits (1645), Expect = 9.2e-182
Identity = 319/458 (69.65%), Postives = 361/458 (78.82%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D   Y E+DPTGRYGRYN++LGKGASKTVYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQ+R KH RVNI AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KPE+LYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELE-GIASTAAH 316
           VKD  +RQFVEKCLA  S RLSARELL DPFLQ+DD      L    G+ +  + +    
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDD------LVFCPGDGDYSLMNYLRQ 312

Query: 317 PFLERERS---------FRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQLENLDNS 376
           P+LE   S           SI       D W C     + DGI+LF   ED+ L N+D +
Sbjct: 313 PYLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDIT 372

Query: 377 IKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIA 436
           IKG+  EDGSI +RLRIAD +G +RNIYFPFD E DTA +VATEM+AELDITD +VT+IA
Sbjct: 373 IKGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIA 432

Query: 437 EKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSS 465
           E IDGE+++LVP+W+PGPGI+E+   +Y   C S  SS
Sbjct: 433 EMIDGEVSALVPDWRPGPGIEESQDTTYCHNCGSNVSS 464

BLAST of Sed0016060 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 9.2e-182
Identity = 319/458 (69.65%), Postives = 361/458 (78.82%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D   Y E+DPTGRYGRYN++LGKGASKTVYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQ+R KH RVNI AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KPE+LYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELE-GIASTAAH 316
           VKD  +RQFVEKCLA  S RLSARELL DPFLQ+DD      L    G+ +  + +    
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDD------LVFCPGDGDYSLMNYLRQ 312

Query: 317 PFLERERS---------FRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQLENLDNS 376
           P+LE   S           SI       D W C     + DGI+LF   ED+ L N+D +
Sbjct: 313 PYLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDIT 372

Query: 377 IKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIA 436
           IKG+  EDGSI +RLRIAD +G +RNIYFPFD E DTA +VATEM+AELDITD +VT+IA
Sbjct: 373 IKGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIA 432

Query: 437 EKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSS 465
           E IDGE+++LVP+W+PGPGI+E+   +Y   C S  SS
Sbjct: 433 EMIDGEVSALVPDWRPGPGIEESQDTTYCHNCGSNVSS 464

BLAST of Sed0016060 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 2.0e-176
Identity = 350/637 (54.95%), Postives = 426/637 (66.88%), Query Frame = 0

Query: 16  PDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 75
           PD+S +VE+DPTGRYGRYNE+LGKGASKTVYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 76  RLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVK 135
           RLY EIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTE+FTSGTLRQ+RL+H+RVNIRA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 136 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 195
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 196 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLY 255
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKP++LY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 256 KVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELEGIASTAAH 315
           KVKD  ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  +    +  +     H
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQPHH 308

Query: 316 -----------PFLERERS---FRSITYSL------------EASDEWRCRTVQT-EPDG 375
                        L R+ S   + S + SL            E  + W     +T E  G
Sbjct: 309 LPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHG 368

Query: 376 IELFEN-DEDDQLE-----NLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTEND 435
           IELFE+ + DDQ E     N+D +IKGK R+DG + +RLRIADKEG++RNIYFPFD E D
Sbjct: 369 IELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETD 428

Query: 436 TAFTVATEMIAELDITDQDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYD 495
           TA +VATEM+AELD+ D  VTKIA  IDGEI+SLVP W+PGP  +E    +      S  
Sbjct: 429 TALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAASI- 488

Query: 496 SSNQPFDDSSKGNEQNVAKLYQILNLSTVER---ASVHRHFDQDFLKPDHPPQLVVENPP 555
            +N   + +S G+  +  +     N+    R      H  F++  ++             
Sbjct: 489 CNNCVSNRTSMGSVMDFLRTNPGANVIQCCRNGCGETHGRFEEITIRE------------ 548

Query: 556 KVSSQHYQPNSILHENQARSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIART 613
             +    +    L + Q     S   S  +H+ ++  + +  ED ++       N I   
Sbjct: 549 --TEVRLRELWKLQQQQESRELSSIDSGHNHSEEEEEEEVLYEDPENMFSCEAGNEINHI 608

BLAST of Sed0016060 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 6.2e-162
Identity = 287/433 (66.28%), Postives = 346/433 (79.91%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D+S YVE+DPTGRYGRYNE+LGKG+SKTVYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
           LY EIHLLKTLKHK+IMKFY SWVDT +RNINFVTEMFTSGTLRQ+RLKH+RVNIRAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKP+ L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELEGIASTAAHP 316
           VKD  +R F+EKCLA VS RLSA ELL D FL ID+ + +   +E +G ++   +   H 
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESDMRRVESE-KGLIDEAGTPLRHS 310

Query: 317 FLERERSFRSITYSLEASDEWRC---RTVQTEPDGIELFENDEDDQLE-----NLDNSIK 376
           +      + +  YSL   ++W      TV++    +  F+ND+D++ E     ++D SIK
Sbjct: 311 Y--HIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIK 370

Query: 377 GKIREDG-SIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAE 436
           GK R++G  + +RL+  +KEG +RNIYFPFD E DTA +VA EM+ EL++ D+DVTKIA 
Sbjct: 371 GKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVTKIAN 430

Query: 437 KIDGEIASLVPEW 441
            IDGEIASLVP W
Sbjct: 431 MIDGEIASLVPNW 440

BLAST of Sed0016060 vs. ExPASy Swiss-Prot
Match: Q8S8Y9 (Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.5e-160
Identity = 300/524 (57.25%), Postives = 373/524 (71.18%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D S +VEIDP+GRYGRY+EILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
            + EIHLLKTL H+NIMKFYTSWVDT + +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKPE+ Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLTEFRGELEGIASTAAH 316
           VKD  +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  ++    +       H
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 317 PFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQLENLDNSIKGKIREDG 376
           P ++         Y  +      C         I+LF ND++D   ++D SIKGK   D 
Sbjct: 310 PLID------DPLYHDQFESSQICE--------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 377 SIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAEKIDGEIAS 436
            I +RLRI+D EG+IRNIYFPF+T  DTA++VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 429

Query: 437 LVPEWK----PGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVAKLYQILNLSTV 496
           LVP+WK        ++     +  G C    +SN    ++    E++    ++  +    
Sbjct: 430 LVPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDK 489

Query: 497 ERASVHRHF-DQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHE 535
             +SVH  F D   L+  +      ++  KV S  +  N +  E
Sbjct: 490 SCSSVHGRFADMWGLRESYSDDGEKQSSRKVRSGRWSENEMRRE 515

BLAST of Sed0016060 vs. ExPASy TrEMBL
Match: A0A6J1KJY6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495945 PE=4 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.4e-297
Identity = 522/615 (84.88%), Postives = 549/615 (89.27%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL GIA T AHPFLER+ SF SI Y LEASDEWRCR+VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L EN A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. ExPASy TrEMBL
Match: A0A6J1F099 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441166 PE=4 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 2.0e-296
Identity = 519/615 (84.39%), Postives = 548/615 (89.11%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           ME  DS +   +LL+ PDFS YVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKP++LYKVKD SMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           +    EL+GIA T AHPFLER+ SF SI Y LEASDEWRC +VQTEPDG ELFEND+DDQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIV+RLRIADKEG+IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+D++N   D S  GN QN 
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARS 540
            KLYQILNLSTVERASVH  F QD LK D PP+ VVE+PPKVSSQHYQPNS+L E+ A  
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCED 600
           S SFR S  + N+KK GQSI V D K+K++D   N IARTSLR   GSRSLSTV SY ED
Sbjct: 541 SHSFRHSHSNQNFKKIGQSIIVGDDKEKILDK--NAIARTSLRSLLGSRSLSTVPSYRED 600

Query: 601 KFASQIHWEIRWLWN 615
           KFASQIH EIRWLWN
Sbjct: 601 KFASQIHREIRWLWN 613

BLAST of Sed0016060 vs. ExPASy TrEMBL
Match: A0A1S3CI45 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103501021 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.7e-285
Identity = 502/617 (81.36%), Postives = 545/617 (88.33%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           M+ TDS ++  HLL+ PDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA++NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAIL+KSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP++LYKVKD SMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKI 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           ++ R EL+  AST   PFLERE+ F SI+YSLEASDEWR R+VQ E DGIELFE++++DQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           LENLD +IKGKIREDGSIV+RLRI DKEG IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY++SNQP D+    N+ N 
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQD--FLKPDHPPQLVVENPPKVSSQHYQPNSILHENQA 540
            KLYQILNLST   A  H+HF+Q+   LK D P Q VV + P VSSQHYQP+S+L ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQA 540

Query: 541 RSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYC 600
            SSRSFRQ  +D NYKKT QS+ V   K+K+ D +A VI  TS R   GSRSLS V SYC
Sbjct: 541 LSSRSFRQRHYDDNYKKTDQSLAV-GYKEKLPDKKATVI-DTSQRSLLGSRSLSRVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 615

BLAST of Sed0016060 vs. ExPASy TrEMBL
Match: A0A0A0M0J2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G695390 PE=4 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.7e-282
Identity = 497/617 (80.55%), Postives = 539/617 (87.36%), Query Frame = 0

Query: 1   MEETDS-SSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60
           M+ TDS ++  HLL+ PDFSHYVEIDPTGRYGRY+EILGKGASKTVYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYDEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA++NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP +LYKVKD SMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDGCESKLKI 300

Query: 301 TEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQ 360
           ++ R EL+  AST   PFLERE+ F SI+YSLE SDEWR R+VQ EPDGIELFE++++DQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEGSDEWRYRSVQKEPDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITD 420
           L +LDN+IKGKIREDGSIV+RLRI DKEG IRNIYFPFDT+NDTA TVATEMIAELDITD
Sbjct: 361 LVSLDNNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNV 480
           QDV KIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY++ NQP D+    N+ N 
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNACNQPSDNILIENKGNG 480

Query: 481 AKLYQILNLSTVERASVHRHFDQD--FLKPDHPPQLVVENPPKVSSQHYQPNSILHENQA 540
            KLYQILNLST   A  H HF+Q+   LK D P Q      P VSSQHYQP+S+L+ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHEHFEQEQFSLKADRPTQ------PNVSSQHYQPDSVLNENQA 540

Query: 541 RSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYC 600
            SS SFRQ   D NYKK  QS+TV   K+K+  ++A VI  TS R   GSRSLSTV SYC
Sbjct: 541 LSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPVNKATVI-DTSQRSLLGSRSLSTVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 615
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 610

BLAST of Sed0016060 vs. ExPASy TrEMBL
Match: A0A6J1DKV6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020998 PE=4 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.6e-280
Identity = 496/614 (80.78%), Postives = 529/614 (86.16%), Query Frame = 0

Query: 1   MEETDSSSHLHLLRLPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWN 60
           ME+ +S      L  PD S YVEIDP+GRYGRYNEILGKGASKTVYRAFDEYEGIEVAWN
Sbjct: 1   MEKDESIDGFSNLHHPDSSRYVEIDPSGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWN 60

Query: 61  QVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLR 120
           QVKLCN LQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTEMFTSG+LR
Sbjct: 61  QVKLCNLLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGSLR 120

Query: 121 QFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLG 180
           QFRLKHRRVN RAVKRWCRQILR LLYLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDLG
Sbjct: 121 QFRLKHRRVNNRAVKRWCRQILRALLYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLG 180

Query: 181 LAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQ 240
           LAAILRKSHAD CVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQ
Sbjct: 181 LAAILRKSHADRCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQ 240

Query: 241 IYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLT 300
           IYKKV SG+KP +LYKVKD SMRQFVEKCLAPV  RLSARELLSDPFLQIDDCESKSK++
Sbjct: 241 IYKKVTSGEKPAALYKVKDPSMRQFVEKCLAPVPYRLSARELLSDPFLQIDDCESKSKIS 300

Query: 301 EFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQL 360
           + +GEL+ IAST   PFLERE SF SI YSL+ SDEWRCR V+TEPDG ELFE+D+DDQL
Sbjct: 301 DCQGELDRIASTIVQPFLEREISFSSINYSLDTSDEWRCRIVETEPDGTELFEDDDDDQL 360

Query: 361 ENLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQ 420
           EN+DNSI GKIREDGSIV+RLRIADKEG+IRNIYFPFDT+ DTA TVATEMIAELDITDQ
Sbjct: 361 ENVDNSITGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKMDTALTVATEMIAELDITDQ 420

Query: 421 DVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVA 480
           DVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDG  QSYD+SN P  +      QN A
Sbjct: 421 DVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGDLQSYDTSNHPSYNHLIEKMQNGA 480

Query: 481 KLYQILNLSTVERASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARSS 540
           K  QILN S  ERAS  RHF+Q   K D P Q VVE  PKVS+QHYQ NS+LHE QA SS
Sbjct: 481 KNSQILNCSADERASAPRHFEQVSHKEDQPTQPVVERIPKVSNQHYQLNSVLHEGQALSS 540

Query: 541 RSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRSYCEDK 600
            SFRQS  D N KKTGQ IT  +  +K+  S+A  IA++S+R   GSRSLS V SY EDK
Sbjct: 541 HSFRQSHSDQNNKKTGQLITGGNDIEKIQVSKATAIAKSSIRSLLGSRSLSRVPSYREDK 600

Query: 601 FASQIHWEIRWLWN 615
           FASQIHWEIRWLWN
Sbjct: 601 FASQIHWEIRWLWN 614

BLAST of Sed0016060 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 620.5 bits (1599), Expect = 1.4e-177
Identity = 350/637 (54.95%), Postives = 426/637 (66.88%), Query Frame = 0

Query: 16  PDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 75
           PD+S +VE+DPTGRYGRYNE+LGKGASKTVYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 76  RLYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVK 135
           RLY EIHLLKTLKHKNIMKFYTSWVDTA+RNINFVTE+FTSGTLRQ+RL+H+RVNIRA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 136 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 195
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 196 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLY 255
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKP++LY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 256 KVKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELEGIASTAAH 315
           KVKD  ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  +    +  +     H
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQPHH 308

Query: 316 -----------PFLERERS---FRSITYSL------------EASDEWRCRTVQT-EPDG 375
                        L R+ S   + S + SL            E  + W     +T E  G
Sbjct: 309 LPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHG 368

Query: 376 IELFEN-DEDDQLE-----NLDNSIKGKIREDGSIVIRLRIADKEGQIRNIYFPFDTEND 435
           IELFE+ + DDQ E     N+D +IKGK R+DG + +RLRIADKEG++RNIYFPFD E D
Sbjct: 369 IELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETD 428

Query: 436 TAFTVATEMIAELDITDQDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYD 495
           TA +VATEM+AELD+ D  VTKIA  IDGEI+SLVP W+PGP  +E    +      S  
Sbjct: 429 TALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAASI- 488

Query: 496 SSNQPFDDSSKGNEQNVAKLYQILNLSTVER---ASVHRHFDQDFLKPDHPPQLVVENPP 555
            +N   + +S G+  +  +     N+    R      H  F++  ++             
Sbjct: 489 CNNCVSNRTSMGSVMDFLRTNPGANVIQCCRNGCGETHGRFEEITIRE------------ 548

Query: 556 KVSSQHYQPNSILHENQARSSRSFRQSQFDHNYKKTGQSITVEDKKDKVIDSRANVIART 613
             +    +    L + Q     S   S  +H+ ++  + +  ED ++       N I   
Sbjct: 549 --TEVRLRELWKLQQQQESRELSSIDSGHNHSEEEEEEEVLYEDPENMFSCEAGNEINHI 608

BLAST of Sed0016060 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 572.4 bits (1474), Expect = 4.4e-163
Identity = 287/433 (66.28%), Postives = 346/433 (79.91%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D+S YVE+DPTGRYGRYNE+LGKG+SKTVYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
           LY EIHLLKTLKHK+IMKFY SWVDT +RNINFVTEMFTSGTLRQ+RLKH+RVNIRAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKP+ L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLTEFRGELEGIASTAAHP 316
           VKD  +R F+EKCLA VS RLSA ELL D FL ID+ + +   +E +G ++   +   H 
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESDMRRVESE-KGLIDEAGTPLRHS 310

Query: 317 FLERERSFRSITYSLEASDEWRC---RTVQTEPDGIELFENDEDDQLE-----NLDNSIK 376
           +      + +  YSL   ++W      TV++    +  F+ND+D++ E     ++D SIK
Sbjct: 311 Y--HIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIK 370

Query: 377 GKIREDG-SIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAE 436
           GK R++G  + +RL+  +KEG +RNIYFPFD E DTA +VA EM+ EL++ D+DVTKIA 
Sbjct: 371 GKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVTKIAN 430

Query: 437 KIDGEIASLVPEW 441
            IDGEIASLVP W
Sbjct: 431 MIDGEIASLVPNW 440

BLAST of Sed0016060 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 565.5 bits (1456), Expect = 5.4e-161
Identity = 300/524 (57.25%), Postives = 373/524 (71.18%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D S +VEIDP+GRYGRY+EILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
            + EIHLLKTL H+NIMKFYTSWVDT + +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 196
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 197 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPESLYK 256
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKPE+ Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 257 VKDLSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLTEFRGELEGIASTAAH 316
           VKD  +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  ++    +       H
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 317 PFLERERSFRSITYSLEASDEWRCRTVQTEPDGIELFENDEDDQLENLDNSIKGKIREDG 376
           P ++         Y  +      C         I+LF ND++D   ++D SIKGK   D 
Sbjct: 310 PLID------DPLYHDQFESSQICE--------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 377 SIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAEKIDGEIAS 436
            I +RLRI+D EG+IRNIYFPF+T  DTA++VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 429

Query: 437 LVPEWK----PGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVAKLYQILNLSTV 496
           LVP+WK        ++     +  G C    +SN    ++    E++    ++  +    
Sbjct: 430 LVPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDK 489

Query: 497 ERASVHRHF-DQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHE 535
             +SVH  F D   L+  +      ++  KV S  +  N +  E
Sbjct: 490 SCSSVHGRFADMWGLRESYSDDGEKQSSRKVRSGRWSENEMRRE 515

BLAST of Sed0016060 vs. TAIR 10
Match: AT3G04910.2 (with no lysine (K) kinase 1 )

HSP 1 Score: 563.5 bits (1451), Expect = 2.0e-160
Identity = 325/608 (53.45%), Postives = 398/608 (65.46%), Query Frame = 0

Query: 45  VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAH 104
           +YRAFDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTA+
Sbjct: 15  LYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTAN 74

Query: 105 RNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDN 164
           RNINFVTE+FTSGTLRQ+RL+H+RVNIRA+K WCRQILRGL YLHS +PPVIHRDLKCDN
Sbjct: 75  RNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDN 134

Query: 165 IFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLE 224
           IFVNGNQGE+KIGDLGLAAILRKSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LE
Sbjct: 135 IFVNGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILE 194

Query: 225 MVTFEYPYSECNHPVQIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLS 284
           MVTF+YPYSEC HP QIYKKV+SGKKP++LYKVKD  ++ F+EKCLA VS R+SARELL 
Sbjct: 195 MVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLD 254

Query: 285 DPFLQIDDCESKSKLTEFRGELEGIASTAAH-----------PFLERERS---FRSITYS 344
           DPFL+IDD E   +  +    +  +     H             L R+ S   + S + S
Sbjct: 255 DPFLRIDDGEFDLRSVDMEDSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSS 314

Query: 345 L------------EASDEWRCRTVQT-EPDGIELFEN-DEDDQLE-----NLDNSIKGKI 404
           L            E  + W     +T E  GIELFE+ + DDQ E     N+D +IKGK 
Sbjct: 315 LNRQYSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIKGKR 374

Query: 405 REDGSIVIRLRIADKEGQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAEKIDG 464
           R+DG + +RLRIADKEG++RNIYFPFD E DTA +VATEM+AELD+ D  VTKIA  IDG
Sbjct: 375 RDDGGLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANMIDG 434

Query: 465 EIASLVPEWKPGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVAKLYQILNLSTV 524
           EI+SLVP W+PGP  +E    +      S   +N   + +S G+  +  +     N+   
Sbjct: 435 EISSLVPSWRPGPEFEECLAAAAAANAASI-CNNCVSNRTSMGSVMDFLRTNPGANVIQC 494

Query: 525 ER---ASVHRHFDQDFLKPDHPPQLVVENPPKVSSQHYQPNSILHENQARSSRSFRQSQF 584
            R      H  F++  ++               +    +    L + Q     S   S  
Sbjct: 495 CRNGCGETHGRFEEITIRE--------------TEVRLRELWKLQQQQESRELSSIDSGH 554

Query: 585 DHNYKKTGQSITVEDKKDKVIDSRANVIARTSLRRWTGSRSLSTVRS-YCE---DKFASQ 613
           +H+ ++  + +  ED ++       N I   S     GS S S + S YC+   +K  +Q
Sbjct: 555 NHSEEEEEEEVLYEDPENMFSCEAGNEINHIS-----GSGSFSFMPSKYCDEPSEKTENQ 602

BLAST of Sed0016060 vs. TAIR 10
Match: AT3G22420.2 (with no lysine (K) kinase 2 )

HSP 1 Score: 534.3 bits (1375), Expect = 1.3e-151
Identity = 300/583 (51.46%), Postives = 373/583 (63.98%), Query Frame = 0

Query: 17  DFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 76
           D S +VEIDP+GRYGRY+EILGKGASKTVYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 77  LYSEIHLLKTLKHKNIMKFYTSWVDTAHRNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 136
            + EIHLLKTL H+NIMKFYTSWVDT + +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 137 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCV-- 196
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CV  
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 197 ------------------------------GTPEFMAPEVYEEAYNELVDIYSFGMCVLE 256
                                         GTPEFMAPEVY+E YNELVD+Y+FGMCVLE
Sbjct: 190 SKPSHHWNFIALIMFFTTLDLPLLCLCVVKGTPEFMAPEVYDEEYNELVDVYAFGMCVLE 249

Query: 257 MVTFEYPYSECNHPVQIYKKVISGKKPESLYKVKDLSMRQFVEKCLAPVSCRLSARELLS 316
           MVTF+YPYSEC HP QIYKKV SGKKPE+ Y VKD  +R+FVEKCLA V+CRL+A ELL 
Sbjct: 250 MVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQ 309

Query: 317 DPFLQIDDCES-KSKLTEFRGELEGIASTAAHPFLERERSFRSITYSLEASDEWRCRTVQ 376
           DPFLQ D+ +    +  ++    +       HP ++         Y  +      C    
Sbjct: 310 DPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHPLID------DPLYHDQFESSQICE--- 369

Query: 377 TEPDGIELFENDEDDQLENLDNSIKGKIREDGSIVIRLRIADKE---------------- 436
                I+LF ND++D   ++D SIKGK   D  I +RLRI+D E                
Sbjct: 370 -----IDLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGIVSIFFDSFKIIGLK 429

Query: 437 -----------GQIRNIYFPFDTENDTAFTVATEMIAELDITDQDVTKIAEKIDGEIASL 496
                      G+IRNIYFPF+T  DTA++VA EM++ELDIT+QDV KIAE ID EIA+L
Sbjct: 430 NVTRFRVLGNVGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAAL 489

Query: 497 VPEWK----PGPGIDETPRISYDGGCQSYDSSNQPFDDSSKGNEQNVAKLYQILNLSTVE 535
           VP+WK        ++     +  G C    +SN    ++    E++    ++  +     
Sbjct: 490 VPDWKNDTESSQNVNNNKNNNTAGFCGEC-ASNGYIQETVSSGEKSHHNHHEFDSSEDKS 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023001906.12.8e-29784.88probable serine/threonine-protein kinase WNK9 [Cucurbita maxima][more]
KAG6588353.14.8e-29784.72putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma s... [more]
XP_022933886.14.1e-29684.39probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata][more]
XP_023529957.15.3e-29684.55probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. ... [more]
XP_038880702.12.3e-29183.14probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A2YMV69.2e-18269.65Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3... [more]
Q0D5989.2e-18269.65Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX... [more]
Q9CAV62.0e-17654.95Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
Q2V3386.2e-16266.28Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y97.5e-16057.25Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KJY61.4e-29784.88Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F0992.0e-29684.39Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3CI452.7e-28581.36Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0M0J24.7e-28280.55Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A6J1DKV62.6e-28080.78Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G04910.11.4e-17754.95with no lysine (K) kinase 1 [more]
AT5G28080.24.4e-16366.28Protein kinase superfamily protein [more]
AT3G22420.15.4e-16157.25with no lysine (K) kinase 2 [more]
AT3G04910.22.0e-16053.45with no lysine (K) kinase 1 [more]
AT3G22420.21.3e-15151.46with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 2..186
e-value: 1.8E-14
score: 64.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 23..186
e-value: 1.8E-33
score: 116.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..186
score: 29.573288
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 277..330
e-value: 3.0E-5
score: 24.0
NoneNo IPR availableGENE3D3.10.20.90coord: 273..339
e-value: 7.9E-6
score: 28.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 18..192
e-value: 4.8E-47
score: 161.8
NoneNo IPR availablePANTHERPTHR13902:SF144SERINE/THREONINE-PROTEIN KINASE WNK1coord: 19..512
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 19..512
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 53..65
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 22..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0016060.1Sed0016060.1mRNA
Sed0016060.2Sed0016060.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity