Sed0015984 (gene) Chayote v1

BLAST of Sed0015984 vs. NCBI nr
Match: XP_022157928.1 (tRNA ligase 1 [Momordica charantia])

HSP 1 Score: 2011.5 bits (5210), Expect = 0.0e+00
Identity = 1016/1198 (84.81%), Postives = 1075/1198 (89.73%), Query Frame = 0

Query: 1    MSVSHKRVLCAITLP---------LSSSLTFSSRTRFYIPHSL-LPFKASSPFPLSCHSP 60
            MS SH R+ CAITLP         L +S  F S + F  P SL LP   SSPF LS HS 
Sbjct: 1    MSASH-RIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSR 60

Query: 61   FIMPYNQPRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQ 120
             IMPYNQ   G ++QKWK K K D TS +   AAEVVT+AL KL V+ESGQPHVPISS +
Sbjct: 61   SIMPYNQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSRE 120

Query: 121  FGNVQLTNQVPPGDGHRTIWKPKAYGTTS-GAAVVEAENASAGRTSIQNKENSAGLAAQN 180
            FGN QLTNQVP G G+R IWKPKAYGTTS GAAVVEAE A A  TSI+NK N+AGLAAQN
Sbjct: 121  FGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNTAGLAAQN 180

Query: 181  ------DIFKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLAT 240
                   +FKGNQIE FTVDN TYT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLAT
Sbjct: 181  GTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLAT 240

Query: 241  LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFND 300
            LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGS AAKKQ EFN+
Sbjct: 241  LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNN 300

Query: 301  FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRL 360
            FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVT+LGNGKPKFYSTAEII FCR+WRL
Sbjct: 301  FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTDLGNGKPKFYSTAEIIVFCREWRL 360

Query: 361  PTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILE 420
            PTNHVWLFSSRKSVTSFFA+FDALCEEGTAT+VCKALDEVAEISVPGSKDHIKVQGEILE
Sbjct: 361  PTNHVWLFSSRKSVTSFFAAFDALCEEGTATSVCKALDEVAEISVPGSKDHIKVQGEILE 420

Query: 421  GLVARMVSHESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQN 480
            GLVAR+VSHESSKHMEKVLEEFP+LPD EGG  DLG SLREICAANRSDEKQQIKALLQN
Sbjct: 421  GLVARIVSHESSKHMEKVLEEFPSLPDEEGGGLDLGRSLREICAANRSDEKQQIKALLQN 480

Query: 481  VGSAFCPVHSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKC 540
            VGS+FCP HSDW GDSHSR ADRSV+SKFLQ +P DFSTSKLQEM+RLMRE R PAAFKC
Sbjct: 481  VGSSFCPDHSDWSGDSHSRTADRSVLSKFLQTSPTDFSTSKLQEMIRLMREKRLPAAFKC 540

Query: 541  YYNFHKVGSISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANK 600
            Y+NFHKVGSISND+LFYKMV+HV SDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFKANK
Sbjct: 541  YHNFHKVGSISNDDLFYKMVIHVHSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKANK 600

Query: 601  DKAAEIVKGKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKE 660
            DKAAEI+K K+NLME+EGNG LGRDG ADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKE
Sbjct: 601  DKAAEIMKSKSNLMEVEGNGILGRDGLADEDANLMIKLKFLTYKLRTFLIRNGLSILFKE 660

Query: 661  GPAAYKAYYLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLE 720
            GPAAYKAYYLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGN+QLSS TYLSEAEPFLE
Sbjct: 661  GPAAYKAYYLRQMKLWGTSVGKQRELSKMLDEWAVYLRRKYGNRQLSSATYLSEAEPFLE 720

Query: 721  QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPK 780
            QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQE  PSS +  GKD V K
Sbjct: 721  QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEVAPSSPMLPGKDTVSK 780

Query: 781  ADGLIVFFPGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKP 840
            A+GLIVFFPGIPGCAKSA+CREILNAPGGLGDDRP+ SLMGDLIKGRYWQKV DE RRKP
Sbjct: 781  AEGLIVFFPGIPGCAKSALCREILNAPGGLGDDRPVKSLMGDLIKGRYWQKVVDERRRKP 840

Query: 841  YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQR 900
            YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTD NPFS DALAVFMFRVLQR
Sbjct: 841  YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDGNPFSLDALAVFMFRVLQR 900

Query: 901  VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDN 960
            VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFE ELIDRFG L+K+PLLK DRSPLPDN
Sbjct: 901  VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEDELIDRFGSLVKMPLLKCDRSPLPDN 960

Query: 961  LKTVLEEGLSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEF 1020
            LKT+LEEGLSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGNTEYLNSIQV FE 
Sbjct: 961  LKTILEEGLSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNTEYLNSIQVPFEV 1020

Query: 1021 AVQDVLEQLKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFF 1080
            AVQDVLEQLKKI KGDYK   ++RRKSATIVFAAVSLP+ EIQNLLDTLGK NPHV  F 
Sbjct: 1021 AVQDVLEQLKKIAKGDYKTPISERRKSATIVFAAVSLPVQEIQNLLDTLGKKNPHVESFL 1080

Query: 1081 KKNLKDFTLREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGS 1140
            K++ KD+TL+  HVTLAHKR HGVKAVADYGIF+NKEVPVELT LLFS+KMAAFEA LGS
Sbjct: 1081 KQDYKDYTLKAAHVTLAHKRSHGVKAVADYGIFQNKEVPVELTALLFSDKMAAFEAHLGS 1140

Query: 1141 IEDERVISKNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            +EDERV+SKNEWPH T+WT EGVAAKEANTLP LV EGKATLVE+NPP +ISG V+FF
Sbjct: 1141 VEDERVVSKNEWPHVTLWTREGVAAKEANTLPQLVSEGKATLVELNPPTIISGTVKFF 1197

BLAST of Sed0015984 vs. NCBI nr
Match: XP_022964893.1 (tRNA ligase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 1009/1190 (84.79%), Postives = 1070/1190 (89.92%), Query Frame = 0

Query: 7    RVLCAITLPLSSSLTFSSRT--------RFYIPHSLLPFKAS-SPFPLSCHSPFIMPYNQ 66
            R+ CAITLPLSSS    SR           +I H  L   AS  PF +   S F MPYNQ
Sbjct: 6    RIFCAITLPLSSSPALHSRAFPFVSCSLSHFILHPSLTLPASVFPFTVCRDSRFTMPYNQ 65

Query: 67   PRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQFGNVQLT 126
             RGG ++QKWK K K +  S +   A+EVVT+AL+ L VTES QPH+PI+S QFGN Q T
Sbjct: 66   RRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTESNQPHIPITSVQFGNAQPT 125

Query: 127  NQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAAQ------NDI 186
            N   PG GHR IWKPKAYGTTSGAAVVE E A A  TSI+NK ++A +AA       + +
Sbjct: 126  NLATPGLGHRAIWKPKAYGTTSGAAVVEGEKAPAVGTSIENKGSNAEIAANSSAIALSQL 185

Query: 187  FKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHS 246
             KGNQIE+FTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLATLEVSLKHS
Sbjct: 186  LKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 245

Query: 247  GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMC 306
            GSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWGS A KKQ EFNDFLESNRMC
Sbjct: 246  GSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSVAPKKQAEFNDFLESNRMC 305

Query: 307  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLF 366
            ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYST+EIIAFCRKWRLPTNHVWLF
Sbjct: 306  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTSEIIAFCRKWRLPTNHVWLF 365

Query: 367  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 426
            SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS
Sbjct: 366  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 425

Query: 427  HESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPV 486
            HESSKHMEKVLEEFPALP NEGG  DLGPSLREICAANRSDEKQQIKALLQNVGSAFCP 
Sbjct: 426  HESSKHMEKVLEEFPALPYNEGGGLDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPD 485

Query: 487  HSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVG 546
            HSDWYGDSHSRNADRSVVSKFLQA PADFSTSKLQEMVRLMRE R PAAFKCY+NFHK+G
Sbjct: 486  HSDWYGDSHSRNADRSVVSKFLQAKPADFSTSKLQEMVRLMRERRLPAAFKCYHNFHKIG 545

Query: 547  SISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVK 606
            SISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK NK+K AEIVK
Sbjct: 546  SISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKENKEKTAEIVK 605

Query: 607  GKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAY 666
             KNNLME EGNGT+GRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKEG AAYKAY
Sbjct: 606  SKNNLMETEGNGTVGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILFKEGSAAYKAY 665

Query: 667  YLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQ 726
            YLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEPFLEQYAKRSPQ
Sbjct: 666  YLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEPFLEQYAKRSPQ 725

Query: 727  NQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFF 786
            NQALIGSAGNLVRAEDFLA+VEEGMDEEGDLQKE +A PSS + S KDVVPKA+GLIVFF
Sbjct: 726  NQALIGSAGNLVRAEDFLAVVEEGMDEEGDLQKE-DAAPSSPMLSRKDVVPKAEGLIVFF 785

Query: 787  PGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADK 846
            PGIPGCAKSA+CREILNAPGGLGDDRP+N+LMGDLIKGRYWQKVADE RRKPYSIMLADK
Sbjct: 786  PGIPGCAKSALCREILNAPGGLGDDRPVNTLMGDLIKGRYWQKVADERRRKPYSIMLADK 845

Query: 847  NAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLD 906
            NAPNEEVWRQIEDMCHSTRASAVPV+PDSEGTDSNPFS DALAVFMFRVLQRVNHPGNLD
Sbjct: 846  NAPNEEVWRQIEDMCHSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLD 905

Query: 907  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEG 966
            KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDRSPLPDNLKT+LEEG
Sbjct: 906  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPLPDNLKTILEEG 965

Query: 967  LSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQ 1026
            LSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGN EYLN+IQV FEFAVQ+VLEQ
Sbjct: 966  LSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNAEYLNAIQVPFEFAVQNVLEQ 1025

Query: 1027 LKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFFKKNLKDFT 1086
            LKKI KGDYK+  T+RRKSATIV+AAVSLP+ +IQ+ LDTLG  NP V  F K+  KD+T
Sbjct: 1026 LKKISKGDYKSPITERRKSATIVYAAVSLPVQDIQDALDTLGNKNPQVEAFIKEGYKDYT 1085

Query: 1087 LREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVIS 1146
            L+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR+GSIEDERVIS
Sbjct: 1086 LKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEARVGSIEDERVIS 1145

Query: 1147 KNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            KNEWPH T+WT EG+AAKEANTLP LV EGKATLVE+NPPI+ISGKVQFF
Sbjct: 1146 KNEWPHVTLWTREGIAAKEANTLPQLVSEGKATLVELNPPIIISGKVQFF 1194

BLAST of Sed0015984 vs. NCBI nr
Match: KAG6583500.1 (tRNA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2004.2 bits (5191), Expect = 0.0e+00
Identity = 1008/1190 (84.71%), Postives = 1069/1190 (89.83%), Query Frame = 0

Query: 7    RVLCAITLPLSSSLTFSSRT--------RFYIPHSLLPFKAS-SPFPLSCHSPFIMPYNQ 66
            R+ CAITLPLSSS     R           +I H  L   AS  PF +S  S F MPYNQ
Sbjct: 6    RIFCAITLPLSSSPALHYRAFPFVSCSLSHFILHPSLTLPASVFPFTVSRDSRFTMPYNQ 65

Query: 67   PRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQFGNVQLT 126
             RGG ++QKWK K K +  S +   A+EVVT+AL+ L VTES QPH+PI+S QFGN Q T
Sbjct: 66   RRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTESNQPHIPITSVQFGNAQPT 125

Query: 127  NQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAAQ------NDI 186
            N   PG GHR IWKPKAYGTTSGAAVVE E A A  TSI+NK ++A +AA       + +
Sbjct: 126  NLATPGLGHRAIWKPKAYGTTSGAAVVEGEKAPAVGTSIENKGSNAEIAANSSAIALSQL 185

Query: 187  FKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHS 246
             KGNQIE+FTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLATLEVSLKHS
Sbjct: 186  LKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 245

Query: 247  GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMC 306
            GSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWGS A KKQ EFNDFLESNRMC
Sbjct: 246  GSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSVAPKKQAEFNDFLESNRMC 305

Query: 307  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLF 366
            ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYST+EIIAFCRKWRLPTNHVWLF
Sbjct: 306  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTSEIIAFCRKWRLPTNHVWLF 365

Query: 367  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 426
            SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS
Sbjct: 366  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 425

Query: 427  HESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPV 486
            HESSKHMEKVLEEFPALP NEGG  DLGPSLREICAANRSDEKQQIKALLQNVGSAFCP 
Sbjct: 426  HESSKHMEKVLEEFPALPYNEGGGLDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPD 485

Query: 487  HSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVG 546
            HSDWYGDSHSRNADRSVVSKFLQA PADFSTSKLQEMVRLMRE R PAAFKCY+NFHK+G
Sbjct: 486  HSDWYGDSHSRNADRSVVSKFLQAKPADFSTSKLQEMVRLMRERRLPAAFKCYHNFHKIG 545

Query: 547  SISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVK 606
            SISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK NK+K AEIVK
Sbjct: 546  SISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKENKEKTAEIVK 605

Query: 607  GKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAY 666
             KNNLME EGNGT+GRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKEG AAYKAY
Sbjct: 606  SKNNLMETEGNGTVGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILFKEGSAAYKAY 665

Query: 667  YLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQ 726
            YLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEPFLEQYAKRSPQ
Sbjct: 666  YLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEPFLEQYAKRSPQ 725

Query: 727  NQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFF 786
            NQALIGSAGNLVRAEDFLA+VEEGMDEEGDLQKE +A PSS + S KDVVPKA+GLIVFF
Sbjct: 726  NQALIGSAGNLVRAEDFLAVVEEGMDEEGDLQKE-DAAPSSPMLSRKDVVPKAEGLIVFF 785

Query: 787  PGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADK 846
            PGIPGCAKSA+CREILNAPGGLGDDRP+N+LMGDLIKGRYWQKVADE RRKPYSIMLADK
Sbjct: 786  PGIPGCAKSALCREILNAPGGLGDDRPVNTLMGDLIKGRYWQKVADERRRKPYSIMLADK 845

Query: 847  NAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLD 906
            NAPNEEVWRQIEDMCHSTRASAVPV+PDSEGTDSNPFS DALAVFMFRVL RVNHPGNLD
Sbjct: 846  NAPNEEVWRQIEDMCHSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLHRVNHPGNLD 905

Query: 907  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEG 966
            KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDRSPLPDNLKT+LEEG
Sbjct: 906  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPLPDNLKTILEEG 965

Query: 967  LSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQ 1026
            LSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGN EYLN+IQV FEFAVQ+VLEQ
Sbjct: 966  LSLYKLHTGRHGRADSTKGSYAKEWAKWEKQLRETLFGNAEYLNAIQVPFEFAVQNVLEQ 1025

Query: 1027 LKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFFKKNLKDFT 1086
            LKKI KGDYK+  T+RRKSATIV+AAVSLP+ +IQ+ LDTLG  NP V  F K+  KD+T
Sbjct: 1026 LKKISKGDYKSPITERRKSATIVYAAVSLPVQDIQDALDTLGNKNPQVEAFIKEGYKDYT 1085

Query: 1087 LREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVIS 1146
            L+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR+GSIEDERVIS
Sbjct: 1086 LKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEARVGSIEDERVIS 1145

Query: 1147 KNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            KNEWPH T+WT EG+AAKEANTLP LV EGKATLVE+NPPI+ISGKVQFF
Sbjct: 1146 KNEWPHVTLWTREGIAAKEANTLPQLVSEGKATLVELNPPIIISGKVQFF 1194

BLAST of Sed0015984 vs. NCBI nr
Match: KAG7019255.1 (tRNA ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2003.0 bits (5188), Expect = 0.0e+00
Identity = 1007/1190 (84.62%), Postives = 1068/1190 (89.75%), Query Frame = 0

Query: 7    RVLCAITLPLSSSLTFSSRT--------RFYIPHSLLPFKAS-SPFPLSCHSPFIMPYNQ 66
            R+ CAITLPLSSS    SR           +I H  L   AS  PF +   S F MPYNQ
Sbjct: 6    RIFCAITLPLSSSPALHSRAFPFVSCSLSHFILHPSLTLPASVFPFTVCRDSRFTMPYNQ 65

Query: 67   PRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQFGNVQLT 126
             RGG ++QKWK K K +  S +   A+EVVT+AL+ L VTES QPH+PI+S QFGN Q T
Sbjct: 66   RRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTESNQPHIPITSVQFGNAQPT 125

Query: 127  NQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAAQ------NDI 186
            N   PG GHR IWKPKAYGTTSGAAVVE E A A  TS +NK ++A +AA       + +
Sbjct: 126  NLATPGLGHRAIWKPKAYGTTSGAAVVEGEKAPAVGTSTENKGSNAEIAANSSAIALSQL 185

Query: 187  FKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHS 246
             KGNQIE+FTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLATLEVSLKHS
Sbjct: 186  LKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 245

Query: 247  GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMC 306
            GSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWGS A KKQ EFNDFLESNRMC
Sbjct: 246  GSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSVAPKKQAEFNDFLESNRMC 305

Query: 307  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLF 366
            ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYST+EIIAFCRKWRLPTNHVWLF
Sbjct: 306  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTSEIIAFCRKWRLPTNHVWLF 365

Query: 367  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 426
            SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS
Sbjct: 366  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 425

Query: 427  HESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPV 486
            HESSKHMEKVLEEFPALP NEGG  DLGPSLREICAANRSDEKQQIKALLQNVGSAFCP 
Sbjct: 426  HESSKHMEKVLEEFPALPYNEGGGLDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPD 485

Query: 487  HSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVG 546
            HSDWYGDSHSRNADRSVVSKFLQA PADFSTSKLQEMVRLMRE R PAAFKCY+NFHK+G
Sbjct: 486  HSDWYGDSHSRNADRSVVSKFLQAKPADFSTSKLQEMVRLMRERRLPAAFKCYHNFHKIG 545

Query: 547  SISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVK 606
            SISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK NK+K AEIVK
Sbjct: 546  SISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKENKEKTAEIVK 605

Query: 607  GKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAY 666
             KNNLME EGNGT+GRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKEG AAYKAY
Sbjct: 606  SKNNLMETEGNGTVGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILFKEGSAAYKAY 665

Query: 667  YLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQ 726
            YLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEPFLEQYAKRSPQ
Sbjct: 666  YLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEPFLEQYAKRSPQ 725

Query: 727  NQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFF 786
            NQALIGSAGNLVRAEDFLA+VEEGMDEEGDLQKE +A PSS + S KDVVPKA+GLIVFF
Sbjct: 726  NQALIGSAGNLVRAEDFLAVVEEGMDEEGDLQKE-DAAPSSPMLSRKDVVPKAEGLIVFF 785

Query: 787  PGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADK 846
            PGIPGCAKSA+CREILNAPGGLGDDRP+N+LMGDLIKGRYWQKVADE RRKPYSIMLADK
Sbjct: 786  PGIPGCAKSALCREILNAPGGLGDDRPVNTLMGDLIKGRYWQKVADERRRKPYSIMLADK 845

Query: 847  NAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLD 906
            NAPNEEVWRQIEDMCHSTRASAVPV+PDSEGTDSNPFS DALAVFMFRVL RVNHPGNLD
Sbjct: 846  NAPNEEVWRQIEDMCHSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLHRVNHPGNLD 905

Query: 907  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEG 966
            KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDRSPLPDNLKT+LEEG
Sbjct: 906  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPLPDNLKTILEEG 965

Query: 967  LSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQ 1026
            LSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGN EYLN+IQV FEFAVQ+VLEQ
Sbjct: 966  LSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNAEYLNAIQVPFEFAVQNVLEQ 1025

Query: 1027 LKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFFKKNLKDFT 1086
            LKKI KGDYK+  T+RRKSATIV+AAVSLP+ +IQ+ LDTLG  NP V  F K+  KD+T
Sbjct: 1026 LKKISKGDYKSPITERRKSATIVYAAVSLPVQDIQDALDTLGNKNPQVEAFIKEGYKDYT 1085

Query: 1087 LREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVIS 1146
            L+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR+GSIEDERVIS
Sbjct: 1086 LKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEARVGSIEDERVIS 1145

Query: 1147 KNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            KNEWPH T+WT EG+AAKEANTLP LV EGKATLVE+NPPI+ISGKVQFF
Sbjct: 1146 KNEWPHVTLWTREGIAAKEANTLPQLVSEGKATLVELNPPIIISGKVQFF 1194

BLAST of Sed0015984 vs. NCBI nr
Match: XP_038894223.1 (tRNA ligase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 2001.1 bits (5183), Expect = 0.0e+00
Identity = 1014/1200 (84.50%), Postives = 1073/1200 (89.42%), Query Frame = 0

Query: 1    MSVSHKRVLCAITLP---LSSSLTFSSR---------TRFYIPHSL-LPFKASSPFPLSC 60
            MS S +R+ CAITLP   L +   F+ R         + F +P SL L    SSPFPLS 
Sbjct: 1    MSAS-QRIFCAITLPHPRLYAPSAFNYRAFPFICHPLSHFILPRSLTLAPLTSSPFPLSR 60

Query: 61   HSPFIMPYNQPRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPIS 120
             S FIMPYNQ +GG ++QKWK K K D  S +   AAEVVT+AL KL VTE+ QPHV  S
Sbjct: 61   DSRFIMPYNQRKGGRREQKWKEKAKVDRNSTESEAAAEVVTNALGKLRVTENDQPHVLTS 120

Query: 121  STQFGNVQLTNQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAA 180
            S QFGN QLTNQV PG  HR +WKPKAYGTTSGAA VE E A    TS +NK ++A LAA
Sbjct: 121  SAQFGNAQLTNQVTPGLAHRAVWKPKAYGTTSGAAEVEGEKAPTNGTSTENKGSNAELAA 180

Query: 181  QN------DIFKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGL 240
            QN       +FKGNQIEKFTVDN TYT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGL
Sbjct: 181  QNGAVGLSQLFKGNQIEKFTVDNSTYTRAQIRATFYPKFENEKSDQEIRTRMIEMVSKGL 240

Query: 241  ATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEF 300
            ATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMF+EAWG+ AAKKQ EF
Sbjct: 241  ATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFQEAWGAAAAKKQAEF 300

Query: 301  NDFLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKW 360
            NDFLESNRM ISMELVTAVLGDHGQRPREDYVVVTAVTELG GKPKFYSTAEIIAFCRKW
Sbjct: 301  NDFLESNRMSISMELVTAVLGDHGQRPREDYVVVTAVTELGKGKPKFYSTAEIIAFCRKW 360

Query: 361  RLPTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEI 420
            RLPTNHVWLFSSRKS TSFFA+FDALCEEGTAT+VCKALDEVAEISVPG+KDHIKVQGEI
Sbjct: 361  RLPTNHVWLFSSRKSATSFFAAFDALCEEGTATSVCKALDEVAEISVPGTKDHIKVQGEI 420

Query: 421  LEGLVARMVSHESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALL 480
            LEGLVAR+VSHESSKHMEKVLE+FPALPDNE G  DLGPSLREICAANRSDEKQQIKALL
Sbjct: 421  LEGLVARIVSHESSKHMEKVLEDFPALPDNEVGGLDLGPSLREICAANRSDEKQQIKALL 480

Query: 481  QNVGSAFCPVHSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAF 540
            QNVGSAFCP HSDWYGDSHSRNADRSV+SKFLQANPADFSTSKLQEM+RLMRE R PAAF
Sbjct: 481  QNVGSAFCPDHSDWYGDSHSRNADRSVLSKFLQANPADFSTSKLQEMIRLMREKRLPAAF 540

Query: 541  KCYYNFHKVGSISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKA 600
            KCY+NFHKVGSISNDNLFYKMV+HV SDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK 
Sbjct: 541  KCYHNFHKVGSISNDNLFYKMVIHVHSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKE 600

Query: 601  NKDKAAEIVKGKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVF 660
            NKDK AE+VK KNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+F
Sbjct: 601  NKDK-AELVKSKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILF 660

Query: 661  KEGPAAYKAYYLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPF 720
            KEGPAAYKAYYLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS TYLSEAEPF
Sbjct: 661  KEGPAAYKAYYLRQMKLWGTSAGKQRELSKMLDEWAVYLRRKYGNKQLSSATYLSEAEPF 720

Query: 721  LEQYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVV 780
            LEQYAKRSPQNQALIGSAGNLV+AEDFLAIVEEGMDEEGDLQKEQEA PSS + SGKD V
Sbjct: 721  LEQYAKRSPQNQALIGSAGNLVKAEDFLAIVEEGMDEEGDLQKEQEAAPSSPMLSGKDAV 780

Query: 781  PKADGLIVFFPGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRR 840
            PKA+GLIVFFPGIPGCAKSA+CREILNAPG LGDDRP+N+LMGDLIKGRYWQKVADE RR
Sbjct: 781  PKAEGLIVFFPGIPGCAKSALCREILNAPGALGDDRPVNTLMGDLIKGRYWQKVADERRR 840

Query: 841  KPYSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVL 900
            KPYSIMLADKNAPNEEVWRQIEDMC STRASAVPVVPDSEGTDSNPFS DALAVFMFRVL
Sbjct: 841  KPYSIMLADKNAPNEEVWRQIEDMCRSTRASAVPVVPDSEGTDSNPFSLDALAVFMFRVL 900

Query: 901  QRVNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLP 960
            QRVNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDR+PLP
Sbjct: 901  QRVNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRNPLP 960

Query: 961  DNLKTVLEEGLSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTF 1020
            +NLKT+LEEGLSLYKLHT RHGR DSTKGS+AKEW KWEKQLRETLFGNTEYLN+IQV F
Sbjct: 961  NNLKTILEEGLSLYKLHTSRHGRVDSTKGSYAKEWTKWEKQLRETLFGNTEYLNAIQVPF 1020

Query: 1021 EFAVQDVLEQLKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGG 1080
            EFAVQDVLEQLKKI KGD+K+  T+RRKS  IVFAAV+LP+ EIQNLL TLGK NP V  
Sbjct: 1021 EFAVQDVLEQLKKISKGDFKSPITERRKSGAIVFAAVNLPVQEIQNLLGTLGKKNPRVEA 1080

Query: 1081 FFKKNLKDFTLREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARL 1140
            F K++ KD+TL+  HVTLAHKR HGVK VADYGIFENKEVPVELT LLFS+KMAAFEARL
Sbjct: 1081 FLKEHYKDYTLKGAHVTLAHKRSHGVKGVADYGIFENKEVPVELTALLFSDKMAAFEARL 1140

Query: 1141 GSIEDERVISKNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            GSIE+ERVISKNEWPH T+WT EGVAAKEAN LP LV EGKATLVEINPPI ISG V+FF
Sbjct: 1141 GSIENERVISKNEWPHVTLWTREGVAAKEANALPQLVSEGKATLVEINPPINISGTVKFF 1198

BLAST of Sed0015984 vs. ExPASy Swiss-Prot
Match: Q0WL81 (tRNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=RNL PE=1 SV=1)

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 755/1115 (67.71%), Postives = 900/1115 (80.72%), Query Frame = 0

Query: 74   DNTSMDAAEVVTHALNKLSVTES--GQPHVPISSTQFGNVQLTNQVPPGDGHRTIWKPKA 133
            D+++   AE V +    LS+ ES    P +P  +T    VQ             +WKPK+
Sbjct: 9    DSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQ-----------NLVWKPKS 68

Query: 134  YGTTSGAAVVEAENASAGRTSIQNKENSAGLAA----QNDIFKGNQIEKFTVDNYTYTHA 193
            YGT SG+    +     G+TS  ++  S+G        + IF GN +EKF+VD  TY HA
Sbjct: 69   YGTVSGS----SSATEVGKTSAVSQIGSSGDTKVGLNLSKIFGGNLLEKFSVDKSTYCHA 128

Query: 194  QIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSF 253
            QIRATFYPKFENEK+DQEIR+RMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSF
Sbjct: 129  QIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKGGAYAKNSF 188

Query: 254  GNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMCISMELVTAVLGDHGQRPRE 313
            GNIYTAVGVFVL RMFREAWG+ A KK+ EFNDFLE NRMCISMELVTAVLGDHGQRP +
Sbjct: 189  GNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKNRMCISMELVTAVLGDHGQRPLD 248

Query: 314  DYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLFSSRKSVTSFFASFDALCEE 373
            DYVVVTAVTELGNGKP+FYST+EII+FCRKWRLPTNHVWLFS+RKSVTSFFA+FDALCEE
Sbjct: 249  DYVVVTAVTELGNGKPQFYSTSEIISFCRKWRLPTNHVWLFSTRKSVTSFFAAFDALCEE 308

Query: 374  GTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHMEKVLEEFPALPD 433
            G AT+VC+ALDEVA+ISVP SKDH+KVQGEILEGLVAR+VS +SS+ ME VL + P  P 
Sbjct: 309  GIATSVCRALDEVADISVPASKDHVKVQGEILEGLVARIVSSQSSRDMENVLRDHPP-PP 368

Query: 434  NEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPVHSDWYGD-SHSRNADRSVV 493
             +G   DLG SLREICAA+RS+EKQQ++ALL++VG +FCP   +W+GD SH ++AD+SV+
Sbjct: 369  CDGANLDLGLSLREICAAHRSNEKQQMRALLRSVGPSFCPSDVEWFGDESHPKSADKSVI 428

Query: 494  SKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVGSISNDNLFYKMVVHVQSD 553
            +KFLQ+ PAD+STSKLQEMVRLM+E R PAAFKCY+NFH+   IS DNLFYK+VVHV SD
Sbjct: 429  TKFLQSQPADYSTSKLQEMVRLMKEKRLPAAFKCYHNFHRAEDISPDNLFYKLVVHVHSD 488

Query: 554  SAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVKGKNNLMEIEGNGTLGRDG 613
            S FRRY KEMR+ P LWPLYRGFF+DINLFK+NK +    +K  +N  E +G G   +DG
Sbjct: 489  SGFRRYHKEMRHMPSLWPLYRGFFVDINLFKSNKGRDLMALKSIDNASENDGRGE--KDG 548

Query: 614  FADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAYYLRQMKLWGTSDGKQREL 673
             AD+DANLMIK+KFLTYKLRTFLIRNGLSI+FK+G AAYK YYLRQMK+WGTSDGKQ+EL
Sbjct: 549  LADDDANLMIKMKFLTYKLRTFLIRNGLSILFKDGAAAYKTYYLRQMKIWGTSDGKQKEL 608

Query: 674  SKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFL 733
             KMLDEWA ++RRK GN QLSS+TYLSEAEPFLEQYAKRSP+N  LIGSAGNLVR EDFL
Sbjct: 609  CKMLDEWAAYIRRKCGNDQLSSSTYLSEAEPFLEQYAKRSPKNHILIGSAGNLVRTEDFL 668

Query: 734  AIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFFPGIPGCAKSAICREILNA 793
            AIV+  +DEEGDL K+Q  TP++   + K+ V K +GLIVFFPGIPG AKSA+C+E+LNA
Sbjct: 669  AIVDGDLDEEGDLVKKQGVTPATPEPAVKEAVQKDEGLIVFFPGIPGSAKSALCKELLNA 728

Query: 794  PGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADKNAPNEEVWRQIEDMCHST 853
            PGG GDDRP+++LMGDL+KG+YW KVADE R+KP SIMLADKNAPNE+VWRQIEDMC  T
Sbjct: 729  PGGFGDDRPVHTLMGDLVKGKYWPKVADERRKKPQSIMLADKNAPNEDVWRQIEDMCRRT 788

Query: 854  RASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLYE 913
            RASAVP+V DSEGTD+NP+S DALAVFMFRVLQRVNHPG LDK S NAGYVLLMFYHLYE
Sbjct: 789  RASAVPIVADSEGTDTNPYSLDALAVFMFRVLQRVNHPGKLDKESSNAGYVLLMFYHLYE 848

Query: 914  GKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEGLSLYKLHTRRHGRADSTK 973
            GK+R EFE ELI+RFG LIK+PLLKSDR+PLPD +K+VLEEG+ L+ LH+RRHGR +STK
Sbjct: 849  GKNRNEFESELIERFGSLIKMPLLKSDRTPLPDPVKSVLEEGIDLFNLHSRRHGRLESTK 908

Query: 974  GSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQLKKILKGDYKNLTTDRRK 1033
            G++A EW KWEKQLR+TL  N+EYL+SIQV FE  V  V E+LK I KGDYK  ++++RK
Sbjct: 909  GTYAAEWTKWEKQLRDTLVANSEYLSSIQVPFESMVHQVREELKTIAKGDYKPPSSEKRK 968

Query: 1034 SATIVFAAVSLPILEIQNLLDTLGKINPHVGGFF---KKNLKDFTLREVHVTLAHKRGHG 1093
              +IVFAA++LP  ++ +LL+ L   NP +  F    KK++++  L   HVTLAHKR HG
Sbjct: 969  HGSIVFAAINLPATQVHSLLEKLAAANPTMRSFLEGKKKSIQE-KLERSHVTLAHKRSHG 1028

Query: 1094 VKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVISKNEWPHATIWTTEGV 1153
            V  VA Y    N+EVPVELT L++++KMAA  A +GS++ E V+SKNEWPH T+WT EGV
Sbjct: 1029 VATVASYSQHLNREVPVELTELIYNDKMAALTAHVGSVDGETVVSKNEWPHVTLWTAEGV 1088

Query: 1154 AAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
             AKEANTLP L  EGKA+ + I+PP+ ISG ++FF
Sbjct: 1089 TAKEANTLPQLYLEGKASRLVIDPPVSISGPLEFF 1104

BLAST of Sed0015984 vs. ExPASy TrEMBL
Match: A0A6J1DUP6 (tRNA ligase 1 OS=Momordica charantia OX=3673 GN=LOC111024537 PE=4 SV=1)

HSP 1 Score: 2011.5 bits (5210), Expect = 0.0e+00
Identity = 1016/1198 (84.81%), Postives = 1075/1198 (89.73%), Query Frame = 0

Query: 1    MSVSHKRVLCAITLP---------LSSSLTFSSRTRFYIPHSL-LPFKASSPFPLSCHSP 60
            MS SH R+ CAITLP         L +S  F S + F  P SL LP   SSPF LS HS 
Sbjct: 1    MSASH-RIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSR 60

Query: 61   FIMPYNQPRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQ 120
             IMPYNQ   G ++QKWK K K D TS +   AAEVVT+AL KL V+ESGQPHVPISS +
Sbjct: 61   SIMPYNQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSRE 120

Query: 121  FGNVQLTNQVPPGDGHRTIWKPKAYGTTS-GAAVVEAENASAGRTSIQNKENSAGLAAQN 180
            FGN QLTNQVP G G+R IWKPKAYGTTS GAAVVEAE A A  TSI+NK N+AGLAAQN
Sbjct: 121  FGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNTAGLAAQN 180

Query: 181  ------DIFKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLAT 240
                   +FKGNQIE FTVDN TYT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLAT
Sbjct: 181  GTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLAT 240

Query: 241  LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFND 300
            LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGS AAKKQ EFN+
Sbjct: 241  LEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNN 300

Query: 301  FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRL 360
            FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVT+LGNGKPKFYSTAEII FCR+WRL
Sbjct: 301  FLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTDLGNGKPKFYSTAEIIVFCREWRL 360

Query: 361  PTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILE 420
            PTNHVWLFSSRKSVTSFFA+FDALCEEGTAT+VCKALDEVAEISVPGSKDHIKVQGEILE
Sbjct: 361  PTNHVWLFSSRKSVTSFFAAFDALCEEGTATSVCKALDEVAEISVPGSKDHIKVQGEILE 420

Query: 421  GLVARMVSHESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQN 480
            GLVAR+VSHESSKHMEKVLEEFP+LPD EGG  DLG SLREICAANRSDEKQQIKALLQN
Sbjct: 421  GLVARIVSHESSKHMEKVLEEFPSLPDEEGGGLDLGRSLREICAANRSDEKQQIKALLQN 480

Query: 481  VGSAFCPVHSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKC 540
            VGS+FCP HSDW GDSHSR ADRSV+SKFLQ +P DFSTSKLQEM+RLMRE R PAAFKC
Sbjct: 481  VGSSFCPDHSDWSGDSHSRTADRSVLSKFLQTSPTDFSTSKLQEMIRLMREKRLPAAFKC 540

Query: 541  YYNFHKVGSISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANK 600
            Y+NFHKVGSISND+LFYKMV+HV SDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFKANK
Sbjct: 541  YHNFHKVGSISNDDLFYKMVIHVHSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKANK 600

Query: 601  DKAAEIVKGKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKE 660
            DKAAEI+K K+NLME+EGNG LGRDG ADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKE
Sbjct: 601  DKAAEIMKSKSNLMEVEGNGILGRDGLADEDANLMIKLKFLTYKLRTFLIRNGLSILFKE 660

Query: 661  GPAAYKAYYLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLE 720
            GPAAYKAYYLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGN+QLSS TYLSEAEPFLE
Sbjct: 661  GPAAYKAYYLRQMKLWGTSVGKQRELSKMLDEWAVYLRRKYGNRQLSSATYLSEAEPFLE 720

Query: 721  QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPK 780
            QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQE  PSS +  GKD V K
Sbjct: 721  QYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEVAPSSPMLPGKDTVSK 780

Query: 781  ADGLIVFFPGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKP 840
            A+GLIVFFPGIPGCAKSA+CREILNAPGGLGDDRP+ SLMGDLIKGRYWQKV DE RRKP
Sbjct: 781  AEGLIVFFPGIPGCAKSALCREILNAPGGLGDDRPVKSLMGDLIKGRYWQKVVDERRRKP 840

Query: 841  YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQR 900
            YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTD NPFS DALAVFMFRVLQR
Sbjct: 841  YSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDGNPFSLDALAVFMFRVLQR 900

Query: 901  VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDN 960
            VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFE ELIDRFG L+K+PLLK DRSPLPDN
Sbjct: 901  VNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEDELIDRFGSLVKMPLLKCDRSPLPDN 960

Query: 961  LKTVLEEGLSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEF 1020
            LKT+LEEGLSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGNTEYLNSIQV FE 
Sbjct: 961  LKTILEEGLSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNTEYLNSIQVPFEV 1020

Query: 1021 AVQDVLEQLKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFF 1080
            AVQDVLEQLKKI KGDYK   ++RRKSATIVFAAVSLP+ EIQNLLDTLGK NPHV  F 
Sbjct: 1021 AVQDVLEQLKKIAKGDYKTPISERRKSATIVFAAVSLPVQEIQNLLDTLGKKNPHVESFL 1080

Query: 1081 KKNLKDFTLREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGS 1140
            K++ KD+TL+  HVTLAHKR HGVKAVADYGIF+NKEVPVELT LLFS+KMAAFEA LGS
Sbjct: 1081 KQDYKDYTLKAAHVTLAHKRSHGVKAVADYGIFQNKEVPVELTALLFSDKMAAFEAHLGS 1140

Query: 1141 IEDERVISKNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            +EDERV+SKNEWPH T+WT EGVAAKEANTLP LV EGKATLVE+NPP +ISG V+FF
Sbjct: 1141 VEDERVVSKNEWPHVTLWTREGVAAKEANTLPQLVSEGKATLVELNPPTIISGTVKFF 1197

BLAST of Sed0015984 vs. ExPASy TrEMBL
Match: A0A6J1HM92 (tRNA ligase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1)

HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 1009/1190 (84.79%), Postives = 1070/1190 (89.92%), Query Frame = 0

Query: 7    RVLCAITLPLSSSLTFSSRT--------RFYIPHSLLPFKAS-SPFPLSCHSPFIMPYNQ 66
            R+ CAITLPLSSS    SR           +I H  L   AS  PF +   S F MPYNQ
Sbjct: 6    RIFCAITLPLSSSPALHSRAFPFVSCSLSHFILHPSLTLPASVFPFTVCRDSRFTMPYNQ 65

Query: 67   PRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQFGNVQLT 126
             RGG ++QKWK K K +  S +   A+EVVT+AL+ L VTES QPH+PI+S QFGN Q T
Sbjct: 66   RRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTESNQPHIPITSVQFGNAQPT 125

Query: 127  NQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAAQ------NDI 186
            N   PG GHR IWKPKAYGTTSGAAVVE E A A  TSI+NK ++A +AA       + +
Sbjct: 126  NLATPGLGHRAIWKPKAYGTTSGAAVVEGEKAPAVGTSIENKGSNAEIAANSSAIALSQL 185

Query: 187  FKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHS 246
             KGNQIE+FTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLATLEVSLKHS
Sbjct: 186  LKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 245

Query: 247  GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMC 306
            GSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWGS A KKQ EFNDFLESNRMC
Sbjct: 246  GSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSVAPKKQAEFNDFLESNRMC 305

Query: 307  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLF 366
            ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYST+EIIAFCRKWRLPTNHVWLF
Sbjct: 306  ISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTSEIIAFCRKWRLPTNHVWLF 365

Query: 367  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 426
            SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS
Sbjct: 366  SSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVS 425

Query: 427  HESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPV 486
            HESSKHMEKVLEEFPALP NEGG  DLGPSLREICAANRSDEKQQIKALLQNVGSAFCP 
Sbjct: 426  HESSKHMEKVLEEFPALPYNEGGGLDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPD 485

Query: 487  HSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVG 546
            HSDWYGDSHSRNADRSVVSKFLQA PADFSTSKLQEMVRLMRE R PAAFKCY+NFHK+G
Sbjct: 486  HSDWYGDSHSRNADRSVVSKFLQAKPADFSTSKLQEMVRLMRERRLPAAFKCYHNFHKIG 545

Query: 547  SISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVK 606
            SISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK NK+K AEIVK
Sbjct: 546  SISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKENKEKTAEIVK 605

Query: 607  GKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAY 666
             KNNLME EGNGT+GRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKEG AAYKAY
Sbjct: 606  SKNNLMETEGNGTVGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILFKEGSAAYKAY 665

Query: 667  YLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQ 726
            YLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEPFLEQYAKRSPQ
Sbjct: 666  YLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEPFLEQYAKRSPQ 725

Query: 727  NQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFF 786
            NQALIGSAGNLVRAEDFLA+VEEGMDEEGDLQKE +A PSS + S KDVVPKA+GLIVFF
Sbjct: 726  NQALIGSAGNLVRAEDFLAVVEEGMDEEGDLQKE-DAAPSSPMLSRKDVVPKAEGLIVFF 785

Query: 787  PGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADK 846
            PGIPGCAKSA+CREILNAPGGLGDDRP+N+LMGDLIKGRYWQKVADE RRKPYSIMLADK
Sbjct: 786  PGIPGCAKSALCREILNAPGGLGDDRPVNTLMGDLIKGRYWQKVADERRRKPYSIMLADK 845

Query: 847  NAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLD 906
            NAPNEEVWRQIEDMCHSTRASAVPV+PDSEGTDSNPFS DALAVFMFRVLQRVNHPGNLD
Sbjct: 846  NAPNEEVWRQIEDMCHSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLD 905

Query: 907  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEG 966
            KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDRSPLPDNLKT+LEEG
Sbjct: 906  KASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPLPDNLKTILEEG 965

Query: 967  LSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQ 1026
            LSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGN EYLN+IQV FEFAVQ+VLEQ
Sbjct: 966  LSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNAEYLNAIQVPFEFAVQNVLEQ 1025

Query: 1027 LKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFFKKNLKDFT 1086
            LKKI KGDYK+  T+RRKSATIV+AAVSLP+ +IQ+ LDTLG  NP V  F K+  KD+T
Sbjct: 1026 LKKISKGDYKSPITERRKSATIVYAAVSLPVQDIQDALDTLGNKNPQVEAFIKEGYKDYT 1085

Query: 1087 LREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVIS 1146
            L+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR+GSIEDERVIS
Sbjct: 1086 LKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEARVGSIEDERVIS 1145

Query: 1147 KNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            KNEWPH T+WT EG+AAKEANTLP LV EGKATLVE+NPPI+ISGKVQFF
Sbjct: 1146 KNEWPHVTLWTREGIAAKEANTLPQLVSEGKATLVELNPPIIISGKVQFF 1194

BLAST of Sed0015984 vs. ExPASy TrEMBL
Match: A0A6J1HM43 (tRNA ligase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1)

HSP 1 Score: 1985.7 bits (5143), Expect = 0.0e+00
Identity = 987/1135 (86.96%), Postives = 1045/1135 (92.07%), Query Frame = 0

Query: 53   MPYNQPRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPISSTQFG 112
            MPYNQ RGG ++QKWK K K +  S +   A+EVVT+AL+ L VTES QPH+PI+S QFG
Sbjct: 1    MPYNQRRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTESNQPHIPITSVQFG 60

Query: 113  NVQLTNQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLAAQ---- 172
            N Q TN   PG GHR IWKPKAYGTTSGAAVVE E A A  TSI+NK ++A +AA     
Sbjct: 61   NAQPTNLATPGLGHRAIWKPKAYGTTSGAAVVEGEKAPAVGTSIENKGSNAEIAANSSAI 120

Query: 173  --NDIFKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEV 232
              + + KGNQIE+FTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKGLATLEV
Sbjct: 121  ALSQLLKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEV 180

Query: 233  SLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLE 292
            SLKHSGSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWGS A KKQ EFNDFLE
Sbjct: 181  SLKHSGSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSVAPKKQAEFNDFLE 240

Query: 293  SNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTN 352
            SNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYST+EIIAFCRKWRLPTN
Sbjct: 241  SNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTSEIIAFCRKWRLPTN 300

Query: 353  HVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLV 412
            HVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLV
Sbjct: 301  HVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLV 360

Query: 413  ARMVSHESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGS 472
            ARMVSHESSKHMEKVLEEFPALP NEGG  DLGPSLREICAANRSDEKQQIKALLQNVGS
Sbjct: 361  ARMVSHESSKHMEKVLEEFPALPYNEGGGLDLGPSLREICAANRSDEKQQIKALLQNVGS 420

Query: 473  AFCPVHSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYN 532
            AFCP HSDWYGDSHSRNADRSVVSKFLQA PADFSTSKLQEMVRLMRE R PAAFKCY+N
Sbjct: 421  AFCPDHSDWYGDSHSRNADRSVVSKFLQAKPADFSTSKLQEMVRLMRERRLPAAFKCYHN 480

Query: 533  FHKVGSISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKA 592
            FHK+GSISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK NK+K 
Sbjct: 481  FHKIGSISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFKENKEKT 540

Query: 593  AEIVKGKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPA 652
            AEIVK KNNLME EGNGT+GRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+FKEG A
Sbjct: 541  AEIVKSKNNLMETEGNGTVGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSILFKEGSA 600

Query: 653  AYKAYYLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYA 712
            AYKAYYLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEPFLEQYA
Sbjct: 601  AYKAYYLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEPFLEQYA 660

Query: 713  KRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADG 772
            KRSPQNQALIGSAGNLVRAEDFLA+VEEGMDEEGDLQKE +A PSS + S KDVVPKA+G
Sbjct: 661  KRSPQNQALIGSAGNLVRAEDFLAVVEEGMDEEGDLQKE-DAAPSSPMLSRKDVVPKAEG 720

Query: 773  LIVFFPGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSI 832
            LIVFFPGIPGCAKSA+CREILNAPGGLGDDRP+N+LMGDLIKGRYWQKVADE RRKPYSI
Sbjct: 721  LIVFFPGIPGCAKSALCREILNAPGGLGDDRPVNTLMGDLIKGRYWQKVADERRRKPYSI 780

Query: 833  MLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNH 892
            MLADKNAPNEEVWRQIEDMCHSTRASAVPV+PDSEGTDSNPFS DALAVFMFRVLQRVNH
Sbjct: 781  MLADKNAPNEEVWRQIEDMCHSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLQRVNH 840

Query: 893  PGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKT 952
            PGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFG L+KIPLLKSDRSPLPDNLKT
Sbjct: 841  PGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPLPDNLKT 900

Query: 953  VLEEGLSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQ 1012
            +LEEGLSLYKLHT RHGRADSTKGS+AKEWAKWEKQLRETLFGN EYLN+IQV FEFAVQ
Sbjct: 901  ILEEGLSLYKLHTSRHGRADSTKGSYAKEWAKWEKQLRETLFGNAEYLNAIQVPFEFAVQ 960

Query: 1013 DVLEQLKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVGGFFKKN 1072
            +VLEQLKKI KGDYK+  T+RRKSATIV+AAVSLP+ +IQ+ LDTLG  NP V  F K+ 
Sbjct: 961  NVLEQLKKISKGDYKSPITERRKSATIVYAAVSLPVQDIQDALDTLGNKNPQVEAFIKEG 1020

Query: 1073 LKDFTLREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIED 1132
             KD+TL+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR+GSIED
Sbjct: 1021 YKDYTLKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEARVGSIED 1080

Query: 1133 ERVISKNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
            ERVISKNEWPH T+WT EG+AAKEANTLP LV EGKATLVE+NPPI+ISGKVQFF
Sbjct: 1081 ERVISKNEWPHVTLWTREGIAAKEANTLPQLVSEGKATLVELNPPIIISGKVQFF 1134

BLAST of Sed0015984 vs. ExPASy TrEMBL
Match: A0A1S3CK49 (uncharacterized protein LOC103501711 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501711 PE=4 SV=1)

HSP 1 Score: 1974.9 bits (5115), Expect = 0.0e+00
Identity = 983/1163 (84.52%), Postives = 1048/1163 (90.11%), Query Frame = 0

Query: 26   TRFYIPHSL-LPFKASSPFPLSCHSPFIMPYNQPRGGCKDQKWKAKTKADNT---SMDAA 85
            + F +P SL L    SSPFPLSC S F+MPYNQ RGG  +QKWK K K D +   S  A 
Sbjct: 33   SHFILPRSLTLAPLTSSPFPLSCDSRFVMPYNQRRGGRGEQKWKEKAKVDKSPTESEAAV 92

Query: 86   EVVTHALNKLSVTESGQPHVPISSTQFGNVQLTNQVPPGDGHRTIWKPKAYGTTSGAAVV 145
            EVVT+AL KL VTES Q HV  SS QFGN QLTNQ  PG  HR IWKPKAYGTTSGAAV+
Sbjct: 93   EVVTNALGKLRVTESDQSHVLTSSAQFGNAQLTNQAIPGLAHRAIWKPKAYGTTSGAAVI 152

Query: 146  EAENASAGRTSIQNKENSAGLAAQ------NDIFKGNQIEKFTVDNYTYTHAQIRATFYP 205
            E E AS   TS +NK ++AGLA Q      + +FK NQIEKF VDN TYT AQIRATFYP
Sbjct: 153  EGEKASTNGTSTENKGSNAGLAVQGGAVGLSQLFKSNQIEKFIVDNSTYTQAQIRATFYP 212

Query: 206  KFENEKSDQEIRSRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVG 265
            KFENEKSDQEIR+RMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVG
Sbjct: 213  KFENEKSDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVG 272

Query: 266  VFVLGRMFREAWGSGAAKKQVEFNDFLESNRMCISMELVTAVLGDHGQRPREDYVVVTAV 325
            VFVLGRMFREAWG+ AAKKQ EFNDFL+SNRMCISMELVTAVLGDHGQRPREDYVVVTAV
Sbjct: 273  VFVLGRMFREAWGAEAAKKQAEFNDFLQSNRMCISMELVTAVLGDHGQRPREDYVVVTAV 332

Query: 326  TELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCK 385
            TELG GKPKFYSTAEIIAFCR WRLPTNHVWLFSSRKSVTSFFA+FDALCEEGTAT+VCK
Sbjct: 333  TELGKGKPKFYSTAEIIAFCRNWRLPTNHVWLFSSRKSVTSFFAAFDALCEEGTATSVCK 392

Query: 386  ALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHMEKVLEEFPALPDNEGGEFDL 445
            ALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHM+KVLEEFPA+PDNEGG  DL
Sbjct: 393  ALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHMQKVLEEFPAVPDNEGGGLDL 452

Query: 446  GPSLREICAANRSDEKQQIKALLQNVGSAFCPVHSDWYGDSHSRNADRSVVSKFLQANPA 505
            GPSLREICAANRSDEKQQIKALLQNVG+AFCP HSDWYGDSHSRNADRSV+SKFLQANPA
Sbjct: 453  GPSLREICAANRSDEKQQIKALLQNVGTAFCPDHSDWYGDSHSRNADRSVLSKFLQANPA 512

Query: 506  DFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVGSISNDNLFYKMVVHVQSDSAFRRYQKE 565
            DFSTSKLQEM+RLMRE R PAAFKCY+NFHKV SISNDNLFYKMV+HV SDSAFRRYQKE
Sbjct: 513  DFSTSKLQEMIRLMRERRLPAAFKCYHNFHKVASISNDNLFYKMVIHVHSDSAFRRYQKE 572

Query: 566  MRNKPGLWPLYRGFFMDINLFKANKDKAAEIVKGKNNLMEIEGNGTLGRDGFADEDANLM 625
            MR+KPGLWPLYRGFF+DINLFK NKDKAA +VK K+NLM+ EGNGTLGRDGFADED+NLM
Sbjct: 573  MRHKPGLWPLYRGFFVDINLFKENKDKAAGLVKSKSNLMDTEGNGTLGRDGFADEDSNLM 632

Query: 626  IKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAYYLRQMKLWGTSDGKQRELSKMLDEWAV 685
            IKLKFLTYKLRTFLIRNGLSI+FKEGP AYKAYYLRQMKLWGTS GKQRELSKMLDEWAV
Sbjct: 633  IKLKFLTYKLRTFLIRNGLSILFKEGPVAYKAYYLRQMKLWGTSAGKQRELSKMLDEWAV 692

Query: 686  FLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDE 745
            ++RRKYGNKQLSS TYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDE
Sbjct: 693  YIRRKYGNKQLSSATYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDE 752

Query: 746  EGDLQKEQEATPSSSLHSGKDVVPKADGLIVFFPGIPGCAKSAICREILNAPGGLGDDRP 805
            EGDLQKEQEA PSS + SGKD VPKA+GLIVFFPGIPGCAKSA+CREILNAPG LGDDRP
Sbjct: 753  EGDLQKEQEAAPSSPMLSGKDAVPKAEGLIVFFPGIPGCAKSALCREILNAPGALGDDRP 812

Query: 806  LNSLMGDLIKGRYWQKVADELRRKPYSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVP 865
            +N+LMGDLIKGRYWQKVADE R+KPYSIMLADKNAPNEEVWRQIEDMC STRASAVPV+P
Sbjct: 813  VNTLMGDLIKGRYWQKVADERRKKPYSIMLADKNAPNEEVWRQIEDMCRSTRASAVPVIP 872

Query: 866  DSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEG 925
            DSEGTDSNPFS DALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLY+GKSRREFEG
Sbjct: 873  DSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLYDGKSRREFEG 932

Query: 926  ELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEGLSLYKLHTRRHGRADSTKGSFAKEWAK 985
            ELIDRFG L+K+PLLK DR+PLPD+LK++LEEG+SLYKLHT RHGR DSTKGS+AKEWAK
Sbjct: 933  ELIDRFGSLVKMPLLKPDRNPLPDDLKSILEEGISLYKLHTSRHGRVDSTKGSYAKEWAK 992

Query: 986  WEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQLKKILKGDYKNLTTDRRKSATIVFAAV 1045
            WEKQLRETLF NTEYLN+IQV FE AVQDVLEQLKKI +GDYK+  T+RRKS  IVFAAV
Sbjct: 993  WEKQLRETLFSNTEYLNAIQVPFESAVQDVLEQLKKISEGDYKSPITERRKSGAIVFAAV 1052

Query: 1046 SLPILEIQNLLDTLGKINPHVGGFFKKNLKDFTLREVHVTLAHKRGHGVKAVADYGIFEN 1105
            SLP+ EIQN+L TLGK N  +  F K++ KD+ L+  HVTLAHKR HGVK VADYGIFEN
Sbjct: 1053 SLPVQEIQNVLGTLGKKNSRIEAFLKEHYKDYKLKGAHVTLAHKRSHGVKGVADYGIFEN 1112

Query: 1106 KEVPVELTGLLFSNKMAAFEARLGSIEDERVISKNEWPHATIWTTEGVAAKEANTLPLLV 1165
            KEVPVELT LLFS+KMA FEARLGSIE+ERVISKNEWPH T+WT EGVAAKEAN LP LV
Sbjct: 1113 KEVPVELTALLFSDKMAGFEARLGSIENERVISKNEWPHVTLWTREGVAAKEANALPQLV 1172

Query: 1166 PEGKATLVEINPPIVISGKVQFF 1179
             EGKATLVEINPPI+ISG V+FF
Sbjct: 1173 SEGKATLVEINPPIIISGIVKFF 1195

BLAST of Sed0015984 vs. ExPASy TrEMBL
Match: A0A6J1I3R5 (tRNA ligase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469400 PE=4 SV=1)

HSP 1 Score: 1974.1 bits (5113), Expect = 0.0e+00
Identity = 1001/1201 (83.35%), Postives = 1067/1201 (88.84%), Query Frame = 0

Query: 1    MSVSHKRVLCAITLP---LSSSLTFSSRTRF-YIPHSL--------LPFKASSPFPLSCH 60
            MS  H R+ CAITLP   LS S  F+ R  F +IP+S         L    S  FP +  
Sbjct: 1    MSAPH-RIFCAITLPRHRLSYSSAFNYRVFFPFIPYSFSHRILSPSLTITDSISFPSTVS 60

Query: 61   SP--FIMPYNQPRGGCKDQKWKAKTKADNTSMD---AAEVVTHALNKLSVTESGQPHVPI 120
            S   F+MPYNQ RGG ++QKWK K K +  S +   A++VVT+AL+ L VTES QPH+PI
Sbjct: 61   SDFRFMMPYNQRRGGRREQKWKEKAKVEGISTESEAASQVVTNALSNLRVTESNQPHIPI 120

Query: 121  SSTQFGNVQLTNQVPPGDGHRTIWKPKAYGTTSGAAVVEAENASAGRTSIQNKENSAGLA 180
            +S QFGN Q TN   PG GHR IWKPKAYGTT GAAVVE E ASA  TSI+NK ++A +A
Sbjct: 121  TSVQFGNAQPTNLATPGLGHRAIWKPKAYGTTIGAAVVEGEKASAVGTSIENKGSNAEIA 180

Query: 181  AQ------NDIFKGNQIEKFTVDNYTYTHAQIRATFYPKFENEKSDQEIRSRMIEMVSKG 240
            A       N + KGNQIEKFTVDN  YT AQIRATFYPKFENEKSDQEIR+RMIEMVSKG
Sbjct: 181  ANSSAIALNQLLKGNQIEKFTVDNSAYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKG 240

Query: 241  LATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSGAAKKQVE 300
            LATLEVSLKHSGSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMF+EAWGS A KKQ E
Sbjct: 241  LATLEVSLKHSGSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFQEAWGSVAPKKQAE 300

Query: 301  FNDFLESNRMCISMELVTAVLGDHGQRPREDYVVVTAVTELGNGKPKFYSTAEIIAFCRK 360
            FNDFLESNRMCISMELVTAVLGDHGQRP+EDYVVVTAVTELGNGKPKFYST+EIIAFCRK
Sbjct: 301  FNDFLESNRMCISMELVTAVLGDHGQRPQEDYVVVTAVTELGNGKPKFYSTSEIIAFCRK 360

Query: 361  WRLPTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGE 420
            WRLPTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGE
Sbjct: 361  WRLPTNHVWLFSSRKSVTSFFASFDALCEEGTATTVCKALDEVAEISVPGSKDHIKVQGE 420

Query: 421  ILEGLVARMVSHESSKHMEKVLEEFPALPDNEGGEFDLGPSLREICAANRSDEKQQIKAL 480
            ILEGLVARMVSHESSKHMEKVLEEFPALP NEGG  DL PSLREICAANRSDEKQQIKAL
Sbjct: 421  ILEGLVARMVSHESSKHMEKVLEEFPALPYNEGGGLDLEPSLREICAANRSDEKQQIKAL 480

Query: 481  LQNVGSAFCPVHSDWYGDSHSRNADRSVVSKFLQANPADFSTSKLQEMVRLMRENRFPAA 540
            LQNVGSAFCP HSDWYGDSHSRNADRSVVSKFLQA PADFST KLQEMVRLMRE R PAA
Sbjct: 481  LQNVGSAFCPDHSDWYGDSHSRNADRSVVSKFLQAKPADFSTFKLQEMVRLMRERRLPAA 540

Query: 541  FKCYYNFHKVGSISNDNLFYKMVVHVQSDSAFRRYQKEMRNKPGLWPLYRGFFMDINLFK 600
            FKCY+NFHKVGSISNDNLFYKMV+HVQSDSAFRRYQKEMR+KPGLWPLYRGFF+DINLFK
Sbjct: 541  FKCYHNFHKVGSISNDNLFYKMVIHVQSDSAFRRYQKEMRHKPGLWPLYRGFFVDINLFK 600

Query: 601  ANKDKAAEIVKGKNNLMEIEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIV 660
             NK+KAAEIVK KNNLME EGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSI+
Sbjct: 601  ENKEKAAEIVKSKNNLMETEGNGTLGRDGFADEDANLMIKLKFLTYKLRTFLIRNGLSIL 660

Query: 661  FKEGPAAYKAYYLRQMKLWGTSDGKQRELSKMLDEWAVFLRRKYGNKQLSSTTYLSEAEP 720
            FKEGPAAYKAYYLRQMKLWGTS GKQRELSKMLDEWAV+LRRKYGNKQLSS+ YLSEAEP
Sbjct: 661  FKEGPAAYKAYYLRQMKLWGTSFGKQRELSKMLDEWAVYLRRKYGNKQLSSSIYLSEAEP 720

Query: 721  FLEQYAKRSPQNQALIGSAGNLVRAEDFLAIVEEGMDEEGDLQKEQEATPSSSLHSGKDV 780
            FLEQYAKRSPQNQ LIGSAGNLVRAEDFLA+V+EGMDEEGDLQKE  A PSS + S KDV
Sbjct: 721  FLEQYAKRSPQNQTLIGSAGNLVRAEDFLAVVDEGMDEEGDLQKEDTA-PSSPMLSRKDV 780

Query: 781  VPKADGLIVFFPGIPGCAKSAICREILNAPGGLGDDRPLNSLMGDLIKGRYWQKVADELR 840
            VPKA+GLIVFFPGIPGCAKS++CREILNAPG LGDDRP+N+L GDLIKGRYWQKVADE R
Sbjct: 781  VPKAEGLIVFFPGIPGCAKSSLCREILNAPGALGDDRPVNTLTGDLIKGRYWQKVADERR 840

Query: 841  RKPYSIMLADKNAPNEEVWRQIEDMCHSTRASAVPVVPDSEGTDSNPFSFDALAVFMFRV 900
            RKPYSIMLADKNAPNEEVWRQIEDMCHST ASAVPV+PDSEGTDSNPFS DALAVFMFRV
Sbjct: 841  RKPYSIMLADKNAPNEEVWRQIEDMCHSTGASAVPVIPDSEGTDSNPFSLDALAVFMFRV 900

Query: 901  LQRVNHPGNLDKASPNAGYVLLMFYHLYEGKSRREFEGELIDRFGFLIKIPLLKSDRSPL 960
            LQRVNHPGNLDKASPNAGYVLLMFYH YEGKSRREFEGELIDRFG L+KIPLLKSDRSPL
Sbjct: 901  LQRVNHPGNLDKASPNAGYVLLMFYHFYEGKSRREFEGELIDRFGSLVKIPLLKSDRSPL 960

Query: 961  PDNLKTVLEEGLSLYKLHTRRHGRADSTKGSFAKEWAKWEKQLRETLFGNTEYLNSIQVT 1020
            PDNLKT+LEEGLSLYKLHT RHG  DSTKGS+AKEWA+WEKQLRETLFGN EYLN+IQV 
Sbjct: 961  PDNLKTILEEGLSLYKLHTSRHGWTDSTKGSYAKEWAEWEKQLRETLFGNAEYLNAIQVP 1020

Query: 1021 FEFAVQDVLEQLKKILKGDYKNLTTDRRKSATIVFAAVSLPILEIQNLLDTLGKINPHVG 1080
            FEF+VQ+VLEQLKKI KGDYK+  T+ RKSATIV+AAVSLP+ EIQN LDTLG  NP V 
Sbjct: 1021 FEFSVQNVLEQLKKISKGDYKSPITE-RKSATIVYAAVSLPVQEIQNALDTLGNKNPQVE 1080

Query: 1081 GFFKKNLKDFTLREVHVTLAHKRGHGVKAVADYGIFENKEVPVELTGLLFSNKMAAFEAR 1140
             F K+  KD+TL+  HVTLAHKR HG+KAVADYGIFENKEVPVELT LLFS+KMAAFEAR
Sbjct: 1081 AFIKEGYKDYTLKSAHVTLAHKRSHGIKAVADYGIFENKEVPVELTALLFSDKMAAFEAR 1140

Query: 1141 LGSIEDERVISKNEWPHATIWTTEGVAAKEANTLPLLVPEGKATLVEINPPIVISGKVQF 1179
            +GSIEDERVISKNEWPH T+WT EG+AAKEAN+LP LV EGKATL+E+NPPI+ISGKVQF
Sbjct: 1141 VGSIEDERVISKNEWPHVTLWTREGIAAKEANSLPQLVSEGKATLLELNPPIIISGKVQF 1198

BLAST of Sed0015984 vs. TAIR 10
Match: AT1G07910.1 (RNAligase )

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 755/1115 (67.71%), Postives = 900/1115 (80.72%), Query Frame = 0

Query: 74   DNTSMDAAEVVTHALNKLSVTES--GQPHVPISSTQFGNVQLTNQVPPGDGHRTIWKPKA 133
            D+++   AE V +    LS+ ES    P +P  +T    VQ             +WKPK+
Sbjct: 9    DSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQ-----------NLVWKPKS 68

Query: 134  YGTTSGAAVVEAENASAGRTSIQNKENSAGLAA----QNDIFKGNQIEKFTVDNYTYTHA 193
            YGT SG+    +     G+TS  ++  S+G        + IF GN +EKF+VD  TY HA
Sbjct: 69   YGTVSGS----SSATEVGKTSAVSQIGSSGDTKVGLNLSKIFGGNLLEKFSVDKSTYCHA 128

Query: 194  QIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSF 253
            QIRATFYPKFENEK+DQEIR+RMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSF
Sbjct: 129  QIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKGGAYAKNSF 188

Query: 254  GNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMCISMELVTAVLGDHGQRPRE 313
            GNIYTAVGVFVL RMFREAWG+ A KK+ EFNDFLE NRMCISMELVTAVLGDHGQRP +
Sbjct: 189  GNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKNRMCISMELVTAVLGDHGQRPLD 248

Query: 314  DYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLFSSRKSVTSFFASFDALCEE 373
            DYVVVTAVTELGNGKP+FYST+EII+FCRKWRLPTNHVWLFS+RKSVTSFFA+FDALCEE
Sbjct: 249  DYVVVTAVTELGNGKPQFYSTSEIISFCRKWRLPTNHVWLFSTRKSVTSFFAAFDALCEE 308

Query: 374  GTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHMEKVLEEFPALPD 433
            G AT+VC+ALDEVA+ISVP SKDH+KVQGEILEGLVAR+VS +SS+ ME VL + P  P 
Sbjct: 309  GIATSVCRALDEVADISVPASKDHVKVQGEILEGLVARIVSSQSSRDMENVLRDHPP-PP 368

Query: 434  NEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPVHSDWYGD-SHSRNADRSVV 493
             +G   DLG SLREICAA+RS+EKQQ++ALL++VG +FCP   +W+GD SH ++AD+SV+
Sbjct: 369  CDGANLDLGLSLREICAAHRSNEKQQMRALLRSVGPSFCPSDVEWFGDESHPKSADKSVI 428

Query: 494  SKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVGSISNDNLFYKMVVHVQSD 553
            +KFLQ+ PAD+STSKLQEMVRLM+E R PAAFKCY+NFH+   IS DNLFYK+VVHV SD
Sbjct: 429  TKFLQSQPADYSTSKLQEMVRLMKEKRLPAAFKCYHNFHRAEDISPDNLFYKLVVHVHSD 488

Query: 554  SAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVKGKNNLMEIEGNGTLGRDG 613
            S FRRY KEMR+ P LWPLYRGFF+DINLFK+NK +    +K  +N  E +G G   +DG
Sbjct: 489  SGFRRYHKEMRHMPSLWPLYRGFFVDINLFKSNKGRDLMALKSIDNASENDGRGE--KDG 548

Query: 614  FADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAYYLRQMKLWGTSDGKQREL 673
             AD+DANLMIK+KFLTYKLRTFLIRNGLSI+FK+G AAYK YYLRQMK+WGTSDGKQ+EL
Sbjct: 549  LADDDANLMIKMKFLTYKLRTFLIRNGLSILFKDGAAAYKTYYLRQMKIWGTSDGKQKEL 608

Query: 674  SKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFL 733
             KMLDEWA ++RRK GN QLSS+TYLSEAEPFLEQYAKRSP+N  LIGSAGNLVR EDFL
Sbjct: 609  CKMLDEWAAYIRRKCGNDQLSSSTYLSEAEPFLEQYAKRSPKNHILIGSAGNLVRTEDFL 668

Query: 734  AIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFFPGIPGCAKSAICREILNA 793
            AIV+  +DEEGDL K+Q  TP++   + K+ V K +GLIVFFPGIPG AKSA+C+E+LNA
Sbjct: 669  AIVDGDLDEEGDLVKKQGVTPATPEPAVKEAVQKDEGLIVFFPGIPGSAKSALCKELLNA 728

Query: 794  PGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADKNAPNEEVWRQIEDMCHST 853
            PGG GDDRP+++LMGDL+KG+YW KVADE R+KP SIMLADKNAPNE+VWRQIEDMC  T
Sbjct: 729  PGGFGDDRPVHTLMGDLVKGKYWPKVADERRKKPQSIMLADKNAPNEDVWRQIEDMCRRT 788

Query: 854  RASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLYE 913
            RASAVP+V DSEGTD+NP+S DALAVFMFRVLQRVNHPG LDK S NAGYVLLMFYHLYE
Sbjct: 789  RASAVPIVADSEGTDTNPYSLDALAVFMFRVLQRVNHPGKLDKESSNAGYVLLMFYHLYE 848

Query: 914  GKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEGLSLYKLHTRRHGRADSTK 973
            GK+R EFE ELI+RFG LIK+PLLKSDR+PLPD +K+VLEEG+ L+ LH+RRHGR +STK
Sbjct: 849  GKNRNEFESELIERFGSLIKMPLLKSDRTPLPDPVKSVLEEGIDLFNLHSRRHGRLESTK 908

Query: 974  GSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQLKKILKGDYKNLTTDRRK 1033
            G++A EW KWEKQLR+TL  N+EYL+SIQV FE  V  V E+LK I KGDYK  ++++RK
Sbjct: 909  GTYAAEWTKWEKQLRDTLVANSEYLSSIQVPFESMVHQVREELKTIAKGDYKPPSSEKRK 968

Query: 1034 SATIVFAAVSLPILEIQNLLDTLGKINPHVGGFF---KKNLKDFTLREVHVTLAHKRGHG 1093
              +IVFAA++LP  ++ +LL+ L   NP +  F    KK++++  L   HVTLAHKR HG
Sbjct: 969  HGSIVFAAINLPATQVHSLLEKLAAANPTMRSFLEGKKKSIQE-KLERSHVTLAHKRSHG 1028

Query: 1094 VKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVISKNEWPHATIWTTEGV 1153
            V  VA Y    N+EVPVELT L++++KMAA  A +GS++ E V+SKNEWPH T+WT EGV
Sbjct: 1029 VATVASYSQHLNREVPVELTELIYNDKMAALTAHVGSVDGETVVSKNEWPHVTLWTAEGV 1088

Query: 1154 AAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
             AKEANTLP L  EGKA+ + I+PP+ ISG ++FF
Sbjct: 1089 TAKEANTLPQLYLEGKASRLVIDPPVSISGPLEFF 1104

BLAST of Sed0015984 vs. TAIR 10
Match: AT1G07910.2 (RNAligase )

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 755/1115 (67.71%), Postives = 900/1115 (80.72%), Query Frame = 0

Query: 74   DNTSMDAAEVVTHALNKLSVTES--GQPHVPISSTQFGNVQLTNQVPPGDGHRTIWKPKA 133
            D+++   AE V +    LS+ ES    P +P  +T    VQ             +WKPK+
Sbjct: 9    DSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQ-----------NLVWKPKS 68

Query: 134  YGTTSGAAVVEAENASAGRTSIQNKENSAGLAA----QNDIFKGNQIEKFTVDNYTYTHA 193
            YGT SG+    +     G+TS  ++  S+G        + IF GN +EKF+VD  TY HA
Sbjct: 69   YGTVSGS----SSATEVGKTSAVSQIGSSGDTKVGLNLSKIFGGNLLEKFSVDKSTYCHA 128

Query: 194  QIRATFYPKFENEKSDQEIRSRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSF 253
            QIRATFYPKFENEK+DQEIR+RMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSF
Sbjct: 129  QIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKGGAYAKNSF 188

Query: 254  GNIYTAVGVFVLGRMFREAWGSGAAKKQVEFNDFLESNRMCISMELVTAVLGDHGQRPRE 313
            GNIYTAVGVFVL RMFREAWG+ A KK+ EFNDFLE NRMCISMELVTAVLGDHGQRP +
Sbjct: 189  GNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKNRMCISMELVTAVLGDHGQRPLD 248

Query: 314  DYVVVTAVTELGNGKPKFYSTAEIIAFCRKWRLPTNHVWLFSSRKSVTSFFASFDALCEE 373
            DYVVVTAVTELGNGKP+FYST+EII+FCRKWRLPTNHVWLFS+RKSVTSFFA+FDALCEE
Sbjct: 249  DYVVVTAVTELGNGKPQFYSTSEIISFCRKWRLPTNHVWLFSTRKSVTSFFAAFDALCEE 308

Query: 374  GTATTVCKALDEVAEISVPGSKDHIKVQGEILEGLVARMVSHESSKHMEKVLEEFPALPD 433
            G AT+VC+ALDEVA+ISVP SKDH+KVQGEILEGLVAR+VS +SS+ ME VL + P  P 
Sbjct: 309  GIATSVCRALDEVADISVPASKDHVKVQGEILEGLVARIVSSQSSRDMENVLRDHPP-PP 368

Query: 434  NEGGEFDLGPSLREICAANRSDEKQQIKALLQNVGSAFCPVHSDWYGD-SHSRNADRSVV 493
             +G   DLG SLREICAA+RS+EKQQ++ALL++VG +FCP   +W+GD SH ++AD+SV+
Sbjct: 369  CDGANLDLGLSLREICAAHRSNEKQQMRALLRSVGPSFCPSDVEWFGDESHPKSADKSVI 428

Query: 494  SKFLQANPADFSTSKLQEMVRLMRENRFPAAFKCYYNFHKVGSISNDNLFYKMVVHVQSD 553
            +KFLQ+ PAD+STSKLQEMVRLM+E R PAAFKCY+NFH+   IS DNLFYK+VVHV SD
Sbjct: 429  TKFLQSQPADYSTSKLQEMVRLMKEKRLPAAFKCYHNFHRAEDISPDNLFYKLVVHVHSD 488

Query: 554  SAFRRYQKEMRNKPGLWPLYRGFFMDINLFKANKDKAAEIVKGKNNLMEIEGNGTLGRDG 613
            S FRRY KEMR+ P LWPLYRGFF+DINLFK+NK +    +K  +N  E +G G   +DG
Sbjct: 489  SGFRRYHKEMRHMPSLWPLYRGFFVDINLFKSNKGRDLMALKSIDNASENDGRGE--KDG 548

Query: 614  FADEDANLMIKLKFLTYKLRTFLIRNGLSIVFKEGPAAYKAYYLRQMKLWGTSDGKQREL 673
             AD+DANLMIK+KFLTYKLRTFLIRNGLSI+FK+G AAYK YYLRQMK+WGTSDGKQ+EL
Sbjct: 549  LADDDANLMIKMKFLTYKLRTFLIRNGLSILFKDGAAAYKTYYLRQMKIWGTSDGKQKEL 608

Query: 674  SKMLDEWAVFLRRKYGNKQLSSTTYLSEAEPFLEQYAKRSPQNQALIGSAGNLVRAEDFL 733
             KMLDEWA ++RRK GN QLSS+TYLSEAEPFLEQYAKRSP+N  LIGSAGNLVR EDFL
Sbjct: 609  CKMLDEWAAYIRRKCGNDQLSSSTYLSEAEPFLEQYAKRSPKNHILIGSAGNLVRTEDFL 668

Query: 734  AIVEEGMDEEGDLQKEQEATPSSSLHSGKDVVPKADGLIVFFPGIPGCAKSAICREILNA 793
            AIV+  +DEEGDL K+Q  TP++   + K+ V K +GLIVFFPGIPG AKSA+C+E+LNA
Sbjct: 669  AIVDGDLDEEGDLVKKQGVTPATPEPAVKEAVQKDEGLIVFFPGIPGSAKSALCKELLNA 728

Query: 794  PGGLGDDRPLNSLMGDLIKGRYWQKVADELRRKPYSIMLADKNAPNEEVWRQIEDMCHST 853
            PGG GDDRP+++LMGDL+KG+YW KVADE R+KP SIMLADKNAPNE+VWRQIEDMC  T
Sbjct: 729  PGGFGDDRPVHTLMGDLVKGKYWPKVADERRKKPQSIMLADKNAPNEDVWRQIEDMCRRT 788

Query: 854  RASAVPVVPDSEGTDSNPFSFDALAVFMFRVLQRVNHPGNLDKASPNAGYVLLMFYHLYE 913
            RASAVP+V DSEGTD+NP+S DALAVFMFRVLQRVNHPG LDK S NAGYVLLMFYHLYE
Sbjct: 789  RASAVPIVADSEGTDTNPYSLDALAVFMFRVLQRVNHPGKLDKESSNAGYVLLMFYHLYE 848

Query: 914  GKSRREFEGELIDRFGFLIKIPLLKSDRSPLPDNLKTVLEEGLSLYKLHTRRHGRADSTK 973
            GK+R EFE ELI+RFG LIK+PLLKSDR+PLPD +K+VLEEG+ L+ LH+RRHGR +STK
Sbjct: 849  GKNRNEFESELIERFGSLIKMPLLKSDRTPLPDPVKSVLEEGIDLFNLHSRRHGRLESTK 908

Query: 974  GSFAKEWAKWEKQLRETLFGNTEYLNSIQVTFEFAVQDVLEQLKKILKGDYKNLTTDRRK 1033
            G++A EW KWEKQLR+TL  N+EYL+SIQV FE  V  V E+LK I KGDYK  ++++RK
Sbjct: 909  GTYAAEWTKWEKQLRDTLVANSEYLSSIQVPFESMVHQVREELKTIAKGDYKPPSSEKRK 968

Query: 1034 SATIVFAAVSLPILEIQNLLDTLGKINPHVGGFF---KKNLKDFTLREVHVTLAHKRGHG 1093
              +IVFAA++LP  ++ +LL+ L   NP +  F    KK++++  L   HVTLAHKR HG
Sbjct: 969  HGSIVFAAINLPATQVHSLLEKLAAANPTMRSFLEGKKKSIQE-KLERSHVTLAHKRSHG 1028

Query: 1094 VKAVADYGIFENKEVPVELTGLLFSNKMAAFEARLGSIEDERVISKNEWPHATIWTTEGV 1153
            V  VA Y    N+EVPVELT L++++KMAA  A +GS++ E V+SKNEWPH T+WT EGV
Sbjct: 1029 VATVASYSQHLNREVPVELTELIYNDKMAALTAHVGSVDGETVVSKNEWPHVTLWTAEGV 1088

Query: 1154 AAKEANTLPLLVPEGKATLVEINPPIVISGKVQFF 1179
             AKEANTLP L  EGKA+ + I+PP+ ISG ++FF
Sbjct: 1089 TAKEANTLPQLYLEGKASRLVIDPPVSISGPLEFF 1104