Sed0015965 (gene) Chayote v1

BLAST of Sed0015965 vs. NCBI nr
Match: KAA0034178.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 808/926 (87.26%), Postives = 844/926 (91.14%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F HS P KS  +IRRFS+TSR+S+ Y+D+ R+RK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CS+  NDVKFAR+RRRF  DRR DLSLK SE + SSK KLE PDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRL +S D S++VRW FLAIA SI VV FAT+VHENLSL EQ+N+LETRISNLNIKLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFD G+ED V SPD+L DV TDKR KTLALIAS+ LL APIIILK I Y+SKSRSLDH 
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNK+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCG 660
            L TVREAS +HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDF VPKSAERILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS-------------------------------AKRLPVRDAKTTAH 780
           IRRHLESLPLESFDS                               AKR+PVRDAK TAH
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKATAH 780

Query: 781 RGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840
           +GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED
Sbjct: 781 KGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840

Query: 841 RQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVIGYRSANAERA 896
           RQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRAR+RREVVIGYRSANAERA
Sbjct: 841 RQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERA 900

BLAST of Sed0015965 vs. NCBI nr
Match: TYK15741.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1528.5 bits (3956), Expect = 0.0e+00
Identity = 808/938 (86.14%), Postives = 844/938 (89.98%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F HS P KS  +IRRFS+TSR+S+ Y+D+ R+RK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CS+  NDVKFAR+RRRF  DRR DLSLK SE + SSK KLE PDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRL +S D S++VRW FLAIA SI VV FAT+VHENLSL EQ+N+LETRISNLNIKLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFD G+ED V SPD+L DV TDKR KTLALIAS+ LL APIIILK I Y+SKSRSLDH 
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNK+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCG 660
            L TVREAS +HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDF VPKSAERILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS-------------------------------------------AK 780
           IRRHLESLPLESFDS                                           AK
Sbjct: 721 IRRHLESLPLESFDSVSSVISFHPGSEILILADESVEDSAIQADSRSLATLLLIRDIQAK 780

Query: 781 RLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 840
           R+PVRDAK TAH+GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 781 RMPVRDAKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 840

Query: 841 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREV 896
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRAR+RREV
Sbjct: 841 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 900

BLAST of Sed0015965 vs. NCBI nr
Match: XP_022956408.1 (ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956409.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956410.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956411.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956412.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 803/944 (85.06%), Postives = 839/944 (88.88%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQ F HS P KS  +IRRFSETSRIS+ Y+DH+R+RKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CSST ND KFAR RRR D DRR DLSLK SES+ SSKRKLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRLFRS D +++VRWRFLAIA SI +VIFATL+HEN SL EQ+N LE RISNLNIKLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFDSGSED V SPD+L D   DKR KTLALIAS+ LL API+ILK I   SKSRSL+H 
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEEVSLNKQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           AL TV E S +HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGL 720
           LPKDF VPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDS--------------------------- 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDS                           
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 ----AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840
               AKR+PVR AKT A+RGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD
Sbjct: 781 RDIQAKRMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840

Query: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRA 896
           YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRA
Sbjct: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRA 900

BLAST of Sed0015965 vs. NCBI nr
Match: XP_023528119.1 (ion channel CASTOR-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528197.1 ion channel CASTOR-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1505.3 bits (3896), Expect = 0.0e+00
Identity = 803/944 (85.06%), Postives = 838/944 (88.77%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQ F HS PPKS  +IRRFSETSRIS+ Y+DH+R+RKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPPKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CSST ND KFAR RRR D DRR DLSLK SES+ SSKRKLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRLFRS D +++VRWRFLA A SI +VIFATL+HEN SL EQ+N LE RISNLNIKLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLATAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLF+SGSED V SPD+L D   DKR KTLALIAS+ LL APIIILK I   SKSRSL+H 
Sbjct: 181 NLFESGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIIILKYID--SKSRSLEHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEEVSLNKQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           RSGSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RSGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           AL TV E S +HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGL 720
           LPKDF VPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDS--------------------------- 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDS                           
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 ----AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840
                KR+PVR AKT A+RGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD
Sbjct: 781 RDIQTKRMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840

Query: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRA 896
           YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRA
Sbjct: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRA 900

BLAST of Sed0015965 vs. NCBI nr
Match: KAG6601611.1 (Ion channel CASTOR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1505.3 bits (3896), Expect = 0.0e+00
Identity = 803/944 (85.06%), Postives = 839/944 (88.88%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQ F HS P KS  +IRRFSETSRIS+ Y+DH+R+RKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CSST ND KFAR RRR D DRR DLSLK SES+ SSKRKLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRLFRS D +++VRWRFLAIA SI +VIFATL+HEN SL EQ+N LE RISNLNIKLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFLVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFDSGSED V SPD+L D   DKR KTLALIAS+ LL API+ILK I   SKSRSL+H 
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEEVSLNKQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           AL TV E S +HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGL 720
           LPKDF VPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDS--------------------------- 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDS                           
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 ----AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840
               AKR+PVR AKT A+RGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD
Sbjct: 781 RDIQAKRMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840

Query: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRA 896
           YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRA
Sbjct: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRA 900

BLAST of Sed0015965 vs. ExPASy Swiss-Prot
Match: Q5H8A6 (Ion channel CASTOR OS=Lotus japonicus OX=34305 GN=CASTOR PE=1 SV=1)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 618/933 (66.24%), Postives = 690/933 (73.95%), Query Frame = 0

Query: 1   MSLDSESSPSSS--RDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSS 60
           MSLDSE S SSS  RDWFFP   F  S P    ++ RRF   S              + S
Sbjct: 1   MSLDSEVSVSSSSGRDWFFPSPSFFRSSP---SQYGRRFHTNS-------------NTHS 60

Query: 61  CISDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVS 120
             S ++P           R RRR          +K S + ++S    E P +S  + K S
Sbjct: 61  APSSTYPSG--------IRHRRR----------VKFSRTPTTSSN--EKPQISIVSDKPS 120

Query: 121 DSSRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLR 180
             S+   +L+   +   +F  + ++I+ ++   L+  N  L  Q+N L+  I    ++L 
Sbjct: 121 AISK--NNLNWLSQFGLQFALVTLTIVFLLL--LLLRNTHLESQVNKLQGEI----LRLH 180

Query: 181 ACNLFDSGSEDVVSSPDKLVDVDT-----DKRYKTLALIASVMLLSAPIIILKSIGYVSK 240
           AC+  D+ +   VSS       DT     +   + LAL  S MLL  P+II K I YVS+
Sbjct: 181 ACHQLDTLN---VSSSTAHKSQDTHPCSCENFKRNLALFLSFMLLLIPLIIFKYIDYVSR 240

Query: 241 SRSLDHYLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLV 300
           SR  ++  E+VSLNKQ+AY+VDVF S++PYAKPL LL+ATLLLI LGGL LFGVT + L 
Sbjct: 241 SRLSENISEQVSLNKQIAYRVDVFLSVYPYAKPLVLLVATLLLIFLGGLTLFGVTTEDLG 300

Query: 301 DCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKG 360
            CLWLSWTYVADSGNHA+SEGIGPRLV+VSISFGGMLIFAMMLGLVSD+ISEKFDSLRKG
Sbjct: 301 HCLWLSWTYVADSGNHASSEGIGPRLVAVSISFGGMLIFAMMLGLVSDAISEKFDSLRKG 360

Query: 361 RSEVVEQNHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDF 420
           +SEVVEQNHTLILGWSDKLGSLL+QLAIANESLGGGT+ VMAERDKE+MELDI KMEFDF
Sbjct: 361 KSEVVEQNHTLILGWSDKLGSLLNQLAIANESLGGGTIAVMAERDKEDMELDIGKMEFDF 420

Query: 421 KGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLR 480
           KGTSVICRSGSPLILADLKKVSVSKAR IIV+AEDGNADQSDARALRTVLSLTGV+EGLR
Sbjct: 421 KGTSVICRSGSPLILADLKKVSVSKARTIIVLAEDGNADQSDARALRTVLSLTGVKEGLR 480

Query: 481 GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE 540
           GHIVVE+SDLDNEVLVKLVGG+LVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE
Sbjct: 481 GHIVVEMSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE 540

Query: 541 FYIKRWPQLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAED 600
           FYIKRWPQLDGM F DVLISFP AIPCGIKVAS GGKI+LNP+DSYVLQEGDEVLVIAED
Sbjct: 541 FYIKRWPQLDGMLFEDVLISFPAAIPCGIKVASYGGKIILNPDDSYVLQEGDEVLVIAED 600

Query: 601 DDTYAPAALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSA 660
           DDTYAPA L  VR                                 RGSLPKDF  PKS 
Sbjct: 601 DDTYAPAPLPMVR---------------------------------RGSLPKDFVYPKSP 660

Query: 661 ERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLV 720
           ERIL CGWRRDMEDMI VLDA LAP SELWMFNDVPE EREKKL DGGLDISRLENISLV
Sbjct: 661 ERILFCGWRRDMEDMITVLDASLAPDSELWMFNDVPEKEREKKLIDGGLDISRLENISLV 720

Query: 721 DREGNAVIRRHLESLPLESFDS-------------------------------AKRLPVR 780
           +REGNAVIRRHLESLPLESFDS                               A+RLP  
Sbjct: 721 NREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQARRLPYV 780

Query: 781 DAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMA 840
              +    G+FS+GSWIGEM+QASDK+VIISEILDPRTKNLLSMSKISDYVLSNELVSMA
Sbjct: 781 AMASQTQGGNFSKGSWIGEMKQASDKTVIISEILDPRTKNLLSMSKISDYVLSNELVSMA 840

Query: 841 LAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVIGYR 896
           LAMVAEDRQINDVLEELFAEEGNE+HIR AD+YLREGEE+SFYE++LRAR+RRE++IGYR
Sbjct: 841 LAMVAEDRQINDVLEELFAEEGNEMHIRQADIYLREGEEMSFYEIMLRARQRREILIGYR 853

BLAST of Sed0015965 vs. ExPASy Swiss-Prot
Match: Q75LD5 (Probable ion channel CASTOR OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0843600 PE=2 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 7.1e-308
Identity = 598/936 (63.89%), Postives = 688/936 (73.50%), Query Frame = 0

Query: 1   MSLDSESSPS-SSRDWFFPP-QCFTHSCPPKSHKFIRRFSETSRISQPYS--DHR----- 60
           M LD +SSP+   RDWFFPP   F  S   ++ +    F  TSR S PYS  D R     
Sbjct: 1   MPLDPDSSPAPPHRDWFFPPAPPFLPSSRARTPR--APFPSTSRSSNPYSFPDRRPPPTP 60

Query: 61  RFRKSSSCISDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVS 120
           R R  S                    A RR D    R   ++  +  + +  K  +    
Sbjct: 61  RSRSRSPLPPPEQQKQQQPPPTTPPPAPRRRDP---RYAGVRRGDVRTLTAEKAAAAAAV 120

Query: 121 SSAKKVSDSSRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRIS 180
            +A +V  S    +S   +  +RW  + ++V+ +V+ F++LV  N SLH+Q++ L+ +++
Sbjct: 121 PTAAQVHGS----KSAASATTLRWSGM-VSVAAIVLCFSSLVRSNSSLHDQVHHLKAQLA 180

Query: 181 NLNIKLRACNLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYV 240
               KL++C    S     + S         ++  K  +L+ S+  L AP++ILK +   
Sbjct: 181 EATTKLQSCITESSMDMSSILSYQSNNSTSQNRGLKNFSLLLSLSTLYAPLLILKYMDLF 240

Query: 241 SKSRSLDHYLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDS 300
            K RS     EEV +NK+LAY+VD+F S+ PYAKPL LL+ATLLLI LGGLAL+GV DDS
Sbjct: 241 LKLRSSQDSEEEVPINKRLAYRVDIFLSLQPYAKPLVLLVATLLLIGLGGLALYGVNDDS 300

Query: 301 LVDCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLR 360
           L+DCLWLSWT+VADSGNHAN+EG GP+LVSVSIS GGML+FAMMLGLV+DSISEKFDSLR
Sbjct: 301 LLDCLWLSWTFVADSGNHANAEGFGPKLVSVSISIGGMLVFAMMLGLVTDSISEKFDSLR 360

Query: 361 KGRSEVVEQNHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEF 420
           KGRSEV+EQ+HTL+LGWSDKLGSLL+Q+AIANESLGGGT+VVMAE+DKEEME DIAKMEF
Sbjct: 361 KGRSEVIEQSHTLVLGWSDKLGSLLNQIAIANESLGGGTIVVMAEKDKEEMEADIAKMEF 420

Query: 421 DFKGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREG 480
           D KGT++ICRSGSPLILADLKKVSVSKARAI+V+AE+GNADQSDARALRTVLSLTGV+EG
Sbjct: 421 DLKGTAIICRSGSPLILADLKKVSVSKARAIVVLAEEGNADQSDARALRTVLSLTGVKEG 480

Query: 481 LRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFEN 540
           LRGHIVVELSDLDNEVLVKLVGG+LVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFEN
Sbjct: 481 LRGHIVVELSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFEN 540

Query: 541 CEFYIKRWPQLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIA 600
           CEFYIKRWPQLDGMQF DVLISFPDAIPCGIKVAS GGKI+LNP+D YVLQEGDEVLVIA
Sbjct: 541 CEFYIKRWPQLDGMQFEDVLISFPDAIPCGIKVASYGGKIILNPDDFYVLQEGDEVLVIA 600

Query: 601 EDDDTYAPAALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPK 660
           EDDDTYAPA L                                   V RG LPKDF VPK
Sbjct: 601 EDDDTYAPAPLPK---------------------------------VMRGYLPKDFVVPK 660

Query: 661 SAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENIS 720
           S ERIL CGWRRDMEDMIMVLDAFLAPGSELWMFNDVPE +RE+KL DGGLD SRLENI+
Sbjct: 661 SPERILFCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPEMDRERKLIDGGLDFSRLENIT 720

Query: 721 LVDREGNAVIRRHLESLPLESFDS-------------------------------AKRLP 780
           LV REGNAVIRRHLESLPLESFDS                               AKRLP
Sbjct: 721 LVHREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRLP 780

Query: 781 VRDAKTT-AHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELV 840
            R+A  +   RGSF +GSWIGEMQQASDKSVIISEILDPRTKNLLS+SKISDYVLSNELV
Sbjct: 781 FREAMVSHVTRGSFCEGSWIGEMQQASDKSVIISEILDPRTKNLLSVSKISDYVLSNELV 840

Query: 841 SMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVI 896
           SMALAMVAEDRQINDVLEELFAE+GNE+ IR ADLYLRE EEL+F+EV+LR R+R+E+VI
Sbjct: 841 SMALAMVAEDRQINDVLEELFAEQGNEMQIRPADLYLREDEELNFFEVMLRGRQRKEIVI 893

BLAST of Sed0015965 vs. ExPASy Swiss-Prot
Match: Q6RHR6 (Ion channel DMI1 OS=Medicago truncatula OX=3880 GN=DMI1 PE=1 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 4.6e-259
Identity = 500/843 (59.31%), Postives = 604/843 (71.65%), Query Frame = 0

Query: 92  LKISESDSSSKRKLESPDVSSSAKKV---SDSSRLFRSLDCSIEVRWRFLAIAVSILVVI 151
           L +++ + ++   L  P  SS  K+    S SS +F            +L +   I++V 
Sbjct: 97  LSVNDHNKTTSSLLPQPSSSSITKQQQQHSTSSPIF------------YLLVICCIILVP 156

Query: 152 FATLVHENLSLHEQINDLETRISNLNIKLRACNLFDSGSEDVVSSPDKLVDVDTDKRYKT 211
           ++  +   L+   ++ D+         KL+ C   D  S +  +S  + VD D +   +T
Sbjct: 157 YSAYLQYKLA---KLKDM---------KLQLCGQIDFCSRNGKTSIQEEVDDDDNADSRT 216

Query: 212 LALIASVMLLSAPIIILKSIGY----VSKSRSLDHYLEEVSLNKQLAYKVDVFFSIHPYA 271
           +AL   +  L  P ++ K + Y    ++  R  +   E+V L K++AY VDVFFSI+PYA
Sbjct: 217 IALYIVLFTLILPFVLYKYLDYLPQIINFLRRTESNKEDVPLKKRVAYMVDVFFSIYPYA 276

Query: 272 KPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSI 331
           K LALL ATL LI  GGLAL+ VT  S+ + LW SWTYVAD+GNHA +EG G R+VSVSI
Sbjct: 277 KLLALLCATLFLIAFGGLALYAVTGGSMAEALWHSWTYVADAGNHAETEGTGQRIVSVSI 336

Query: 332 SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLHQLAIANE 391
           S GGMLIFAMMLGLVSD+ISEK DSLRKG+SEV+E+NH LILGWSDKLGSLL QLAIAN+
Sbjct: 337 SAGGMLIFAMMLGLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQLAIANK 396

Query: 392 SLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIV 451
           S+GGG +VV+AE++KEEME+DIAK+EFDF GTSVICRSGSPLILADLKKVSVSKARAIIV
Sbjct: 397 SVGGGVIVVLAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAIIV 456

Query: 452 IAEDGNADQSDARALRTVLSLTGVREGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHD 511
           +A D NADQSDARALR VLSL GV+EGLRGH+VVE+SDLDNE LVKLVGGEL+ETVVAHD
Sbjct: 457 LAADENADQSDARALRVVLSLAGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHD 516

Query: 512 VIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFLDVLISFPDAIPCGIKV 571
           VIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+LD + F D+LISFPDAIPCG+KV
Sbjct: 517 VIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDDLLFKDILISFPDAIPCGVKV 576

Query: 572 ASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALSTVREASSMHIARPTRKPQKIL 631
           A+ GGKIV+NP+D+YVL++GDEVLVIAEDDDTYAP  L  VR                  
Sbjct: 577 AADGGKIVINPDDNYVLRDGDEVLVIAEDDDTYAPGPLPEVR------------------ 636

Query: 632 LCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWM 691
                          +G  P+  + PK  E+IL CGWRRD++DMIMVL+AFLAPGSELWM
Sbjct: 637 ---------------KGYFPRIRDPPKYPEKILFCGWRRDIDDMIMVLEAFLAPGSELWM 696

Query: 692 FNDVPENEREKKLTDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS--------- 751
           FN+VPE ERE+KL  G LD+  LENI LV REGNAVIRRHLESLPLE+FDS         
Sbjct: 697 FNEVPEKERERKLAAGELDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILILADESV 756

Query: 752 ----------------------AKRLPVRDAKTTAHR-GSFSQGSWIGEMQQASDKSVII 811
                                 ++RLP RD K+T+ R   FS  SWI EMQQASDKS+II
Sbjct: 757 EDSVAHSDSRSLATLLLIRDIQSRRLPYRDTKSTSLRLSGFSHNSWIREMQQASDKSIII 816

Query: 812 SEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHA 871
           SEILD RT+NL+S+S+ISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ I+ A
Sbjct: 817 SEILDSRTRNLVSVSRISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIKPA 876

Query: 872 DLYLREGEELSFYEVLLRARRRREVVIGYRSANAERAVINPPAKNERRKWSLRDDFVVIA 896
           + YL + EEL FY++++R R R+E+VIGYR AN ERA+INP  K+  RKWSL D FVV+A
Sbjct: 877 EFYLFDQEELCFYDIMIRGRTRKEIVIGYRLANQERAIINPSEKSVPRKWSLDDVFVVLA 882

BLAST of Sed0015965 vs. ExPASy Swiss-Prot
Match: Q4VY51 (Probable ion channel SYM8 OS=Pisum sativum OX=3888 GN=SYM8 PE=1 SV=3)

HSP 1 Score: 895.6 bits (2313), Expect = 4.6e-259
Identity = 489/774 (63.18%), Postives = 580/774 (74.94%), Query Frame = 0

Query: 166 LETRISNL-NIKLRACNLFDSGSEDVVSSPDKLVDVDTDKRY-------KTLALIASVML 225
           L+ +++ L ++KL+ C   D  S +  +S  K V  D    Y       +T++L   +  
Sbjct: 154 LQYKLAKLKDMKLQLCCQIDFCSGNGKTSLQKDVVDDGSFSYYILNADSRTISLYIVLFT 213

Query: 226 LSAPIIILKSIGY----VSKSRSLDHYLEEVSLNKQLAYKVDVFFSIHPYAKPLALLIAT 285
           L  P I+ K I Y    ++ SR  +   E+V L K++AY VDVFFSI+PYAK LALL AT
Sbjct: 214 LVLPFILYKYIDYLPQMINFSRRTNSNKEDVPLKKRVAYMVDVFFSIYPYAKLLALLFAT 273

Query: 286 LLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFA 345
           L LI  GGLAL+ VT  S+ + LW SWTYVAD+GNHA +EG+G R+VSVSIS GGMLIFA
Sbjct: 274 LFLIAFGGLALYAVTGGSMAEALWHSWTYVADAGNHAETEGMGQRIVSVSISAGGMLIFA 333

Query: 346 MMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLHQLAIANESLGGGTVVV 405
           MMLGLVSD+ISEK DSLRKG+SEV+E+NH LILGWSDKLGSLL QLAIAN+S+GGG +VV
Sbjct: 334 MMLGLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQLAIANKSVGGGVIVV 393

Query: 406 MAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQ 465
           +AE++KEEME+DIAK+EFDF GTSVICRSGSPLILADLKKVSVSKARAIIV+A D NADQ
Sbjct: 394 LAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAIIVLASDENADQ 453

Query: 466 SDARALRTVLSLTGVREGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQC 525
           SDARALR VLSLTGV+E LRGH+VVE+SDLDNE LVKLVGGEL+ETVVAHDVIGRLMIQC
Sbjct: 454 SDARALRVVLSLTGVKEALRGHVVVEMSDLDNEPLVKLVGGELIETVVAHDVIGRLMIQC 513

Query: 526 ARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFLDVLISFPDAIPCGIKVASLGGKIVL 585
           A QPGLAQIWEDILGFEN EFYIKRWP+LDG+ F D+LISFPDAIPCG+KV++ GGKIV+
Sbjct: 514 ALQPGLAQIWEDILGFENAEFYIKRWPELDGLLFKDILISFPDAIPCGVKVSADGGKIVI 573

Query: 586 NPEDSYVLQEGDEVLVIAEDDDTYAPAALSTVREASSMHIARPTRKPQKILLCGWRRDID 645
           NP+D+YVL++GDEVLVIAEDDDTYAP  L  VR                           
Sbjct: 574 NPDDNYVLRDGDEVLVIAEDDDTYAPGPLPEVR--------------------------- 633

Query: 646 DMIVVWRGSLPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENER 705
                 +G  P+  + PK  E+IL CGWRRD++DMIMVL+AFLAPGSELWMFN+VPE +R
Sbjct: 634 ------KGYFPRIRDPPKYPEKILFCGWRRDIDDMIMVLEAFLAPGSELWMFNEVPEKQR 693

Query: 706 EKKLTDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS------------------ 765
           E+KL  G LD+  LENI LV REGNAVIRRHLESLPLE+FDS                  
Sbjct: 694 ERKLAAGELDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILILADESVEDSVAHSDS 753

Query: 766 -------------AKRLPVRDAKTTAHR-GSFSQGSWIGEMQQASDKSVIISEILDPRTK 825
                        ++RLP RD K+T+ R   FS  SWI EMQQASDKS+IISEILD RT+
Sbjct: 754 RSLATLLLIRDIQSRRLPYRDTKSTSLRLSGFSHNSWIREMQQASDKSIIISEILDSRTR 813

Query: 826 NLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEE 885
           NL+S+S+ISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ I+ A+ YL + EE
Sbjct: 814 NLVSVSRISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIKPAEFYLFDQEE 873

Query: 886 LSFYEVLLRARRRREVVIGYRSANAERAVINPPAKNERRKWSLRDDFVVIAEKE 896
           L FY++++R R R+E+VIGYR A+ ERA+INP  K+  RKWSL D FVVIA  E
Sbjct: 874 LCFYDIMIRGRTRKEIVIGYRLASQERALINPSEKSMTRKWSLDDVFVVIASGE 894

BLAST of Sed0015965 vs. ExPASy Swiss-Prot
Match: Q5N941 (Probable ion channel POLLUX OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0870100 PE=2 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 3.0e-258
Identity = 475/720 (65.97%), Postives = 554/720 (76.94%), Query Frame = 0

Query: 209 LALIASVMLLSAPIIILKSIGYVSK----SRSLDHYLEEVSLNKQLAYKVDVFFSIHPYA 268
           LA  + +++ + P+ ++K I  + +    S  L    EEV L K++AYKVDVFFS HPYA
Sbjct: 255 LASCSRMLVFAIPVFLVKYIDQLRRRNTDSIRLRSTEEEVPLKKRIAYKVDVFFSGHPYA 314

Query: 269 KPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSI 328
           K LALL+AT++LI  GG+AL+ V+    ++ LWLSWT+VADSGNHA+  G+GPR+VSVSI
Sbjct: 315 KLLALLLATIILIASGGIALYVVSGSGFLEALWLSWTFVADSGNHADQVGLGPRIVSVSI 374

Query: 329 SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQNHTLILGWSDKLGSLLHQLAIANE 388
           S GGML+FA MLGLVSD+ISEK DS RKG+SEV+E NH LILGWSDKLGSLL QLAIAN+
Sbjct: 375 SSGGMLVFATMLGLVSDAISEKVDSWRKGKSEVIEVNHILILGWSDKLGSLLKQLAIANK 434

Query: 389 SLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIV 448
           S+GGG VVV+AERDKEEME+DI K+EFDF GTSVICRSGSPLILADLKKVSVSKARAIIV
Sbjct: 435 SIGGGVVVVLAERDKEEMEMDIGKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAIIV 494

Query: 449 IAEDGNADQSDARALRTVLSLTGVREGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHD 508
           +A D NADQSDARALR VLSLTGV+EGLRGH+VVE+SDLDNE LVKLVGGEL+ETVVAHD
Sbjct: 495 LASDENADQSDARALRVVLSLTGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHD 554

Query: 509 VIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFLDVLISFPDAIPCGIKV 568
           VIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+LDGM+F DVLISFPDA+PCG+K+
Sbjct: 555 VIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDGMRFGDVLISFPDAVPCGVKI 614

Query: 569 ASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALSTVREASSMHIARPTRKPQKIL 628
           AS  GKI++NP++ YVLQEGDEVLVIAEDDDTY PA+L  VR                  
Sbjct: 615 ASKAGKILMNPDNDYVLQEGDEVLVIAEDDDTYVPASLPQVR------------------ 674

Query: 629 LCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWM 688
                          +G LP     PK  E+IL CGWRRD+ DMIMVL+AFLAPGSELWM
Sbjct: 675 ---------------KGFLPNIPTPPKYPEKILFCGWRRDIHDMIMVLEAFLAPGSELWM 734

Query: 689 FNDVPENEREKKLTDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS--------- 748
           FN+VPE ERE+KLTDGG+DI  L NI LV +EGNAVIRRHLESLPLE+FDS         
Sbjct: 735 FNEVPEKERERKLTDGGMDIYGLTNIKLVHKEGNAVIRRHLESLPLETFDSILILADESV 794

Query: 749 ----------------------AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIIS 808
                                 +KRLP ++ K+      F   SWI EMQ ASDKS+IIS
Sbjct: 795 EDSIVHSDSRSLATLLLIRDIQSKRLPSKELKSPLRYNGFCHSSWIREMQHASDKSIIIS 854

Query: 809 EILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHAD 868
           EILD RT+NL+S+SKISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ IR A+
Sbjct: 855 EILDSRTRNLVSVSKISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIRSAE 914

Query: 869 LYLREGEELSFYEVLLRARRRREVVIGYRSANAERAVINPPAKNERRKWSLRDDFVVIAE 894
            YL E EELSF+++++RAR R EVVIGYR AN ++A+INP  K+E RKWSL D FVVI++
Sbjct: 915 FYLYEQEELSFFDIMVRARERDEVVIGYRLANDDQAIINPEQKSEIRKWSLDDVFVVISK 941

BLAST of Sed0015965 vs. ExPASy TrEMBL
Match: A0A5A7SU61 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001710 PE=3 SV=1)

HSP 1 Score: 1533.1 bits (3968), Expect = 0.0e+00
Identity = 808/926 (87.26%), Postives = 844/926 (91.14%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F HS P KS  +IRRFS+TSR+S+ Y+D+ R+RK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CS+  NDVKFAR+RRRF  DRR DLSLK SE + SSK KLE PDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRL +S D S++VRW FLAIA SI VV FAT+VHENLSL EQ+N+LETRISNLNIKLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFD G+ED V SPD+L DV TDKR KTLALIAS+ LL APIIILK I Y+SKSRSLDH 
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNK+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCG 660
            L TVREAS +HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDF VPKSAERILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS-------------------------------AKRLPVRDAKTTAH 780
           IRRHLESLPLESFDS                               AKR+PVRDAK TAH
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKATAH 780

Query: 781 RGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840
           +GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED
Sbjct: 781 KGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840

Query: 841 RQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVIGYRSANAERA 896
           RQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRAR+RREVVIGYRSANAERA
Sbjct: 841 RQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERA 900

BLAST of Sed0015965 vs. ExPASy TrEMBL
Match: A0A5D3CV00 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002430 PE=3 SV=1)

HSP 1 Score: 1528.5 bits (3956), Expect = 0.0e+00
Identity = 808/938 (86.14%), Postives = 844/938 (89.98%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F HS P KS  +IRRFS+TSR+S+ Y+D+ R+RK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CS+  NDVKFAR+RRRF  DRR DLSLK SE + SSK KLE PDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRL +S D S++VRW FLAIA SI VV FAT+VHENLSL EQ+N+LETRISNLNIKLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFD G+ED V SPD+L DV TDKR KTLALIAS+ LL APIIILK I Y+SKSRSLDH 
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNK+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCG 660
            L TVREAS +HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDF VPKSAERILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS-------------------------------------------AK 780
           IRRHLESLPLESFDS                                           AK
Sbjct: 721 IRRHLESLPLESFDSVSSVISFHPGSEILILADESVEDSAIQADSRSLATLLLIRDIQAK 780

Query: 781 RLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 840
           R+PVRDAK TAH+GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 781 RMPVRDAKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 840

Query: 841 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREV 896
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRAR+RREV
Sbjct: 841 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 900

BLAST of Sed0015965 vs. ExPASy TrEMBL
Match: A0A6J1GWH3 (ion channel CASTOR-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458157 PE=3 SV=1)

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 803/944 (85.06%), Postives = 839/944 (88.88%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQ F HS P KS  +IRRFSETSRIS+ Y+DH+R+RKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CSST ND KFAR RRR D DRR DLSLK SES+ SSKRKLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRLFRS D +++VRWRFLAIA SI +VIFATL+HEN SL EQ+N LE RISNLNIKLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFDSGSED V SPD+L D   DKR KTLALIAS+ LL API+ILK I   SKSRSL+H 
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEEVSLNKQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           AL TV E S +HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGL 720
           LPKDF VPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDS--------------------------- 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDS                           
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 ----AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840
               AKR+PVR AKT A+RGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD
Sbjct: 781 RDIQAKRMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840

Query: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRA 896
           YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRA
Sbjct: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRA 900

BLAST of Sed0015965 vs. ExPASy TrEMBL
Match: A0A6J1K6V9 (ion channel CASTOR-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492175 PE=3 SV=1)

HSP 1 Score: 1498.4 bits (3878), Expect = 0.0e+00
Identity = 800/944 (84.75%), Postives = 834/944 (88.35%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F  S P KS  +IRRFSETSRIS+ Y+DH+R+RKSSSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFVQSYPAKSQTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CSST ND KFAR RRR D DRR DLSLK SE + SSKRKLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSELEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRLFRS D +++VRWRFLAIA SI +VIFATL+HEN SL EQ+N LE RISNLNIKLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFDSGSED V SPD+L D   DKR KTLALI S+ LL APIIILK I   SKSRSL+H 
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALITSLTLLLAPIIILKYID--SKSRSLEHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNKQL YKVDVFFS+HPYAKPLALLIATL LIMLGGLALFGVTDDSLVDCLWLSW
Sbjct: 241 LEEISLNKQLLYKVDVFFSVHPYAKPLALLIATLQLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           RSGSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RSGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           AL TV E S +HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFNVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGL 720
           LPKDF VPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDS--------------------------- 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDS                           
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 ----AKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840
               AKR+PVR AKT  +RGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD
Sbjct: 781 RDIQAKRMPVRGAKTVTYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISD 840

Query: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRA 896
           YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRA
Sbjct: 841 YVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRA 900

BLAST of Sed0015965 vs. ExPASy TrEMBL
Match: A0A1S3BE18 (ion channel CASTOR-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488869 PE=3 SV=1)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 777/926 (83.91%), Postives = 812/926 (87.69%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQCFTHSCPPKSHKFIRRFSETSRISQPYSDHRRFRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQ F HS P KS  +IRRFS+TSR+S+ Y+D+ R+RK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHPCSSTGNDVKFARARRRFDSDRRRDLSLKISESDSSSKRKLESPDVSSSAKKVSDS 120
           SDSH CS+  NDVKFAR+RRRF  DRR DLSLK SE + SSK KLE PDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 SRLFRSLDCSIEVRWRFLAIAVSILVVIFATLVHENLSLHEQINDLETRISNLNIKLRAC 180
           SRL +S D S++VRW FLAIA SI VV FAT+VHENLSL EQ+N+LETRISNLNIKLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLFDSGSEDVVSSPDKLVDVDTDKRYKTLALIASVMLLSAPIIILKSIGYVSKSRSLDHY 240
           NLFD G+ED V SPD+L DV TDKR KTLALIAS+ LL APIIILK I Y+SKSRSLDH 
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300
           LEE+SLNK+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD+LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 NHTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420
           NHTLILGWSDKLGSLL+Q++IANESLGGG VVVMAERDKEEMELDIAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGV+EGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQF DVLISFPDAIPCGIKVAS GGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCG 660
            L T                                 VWRGSLPKDF VPKSAERILLCG
Sbjct: 601 PLPT---------------------------------VWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS-------------------------------AKRLPVRDAKTTAH 780
           IRRHLESLPLESFDS                               AKR+PVRDAK TAH
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQAKRMPVRDAKATAH 780

Query: 781 RGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840
           +GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED
Sbjct: 781 KGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 840

Query: 841 RQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVIGYRSANAERA 896
           RQINDVLEELFAEEGNELHIR ADLYLREGEELSFYEVLLRAR+RREVVIGYRSANAERA
Sbjct: 841 RQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERA 893

BLAST of Sed0015965 vs. TAIR 10
Match: AT5G49960.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1012 (InterPro:IPR010420); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF1012) (TAIR:AT5G02940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 842.8 bits (2176), Expect = 2.5e-244
Identity = 450/686 (65.60%), Postives = 530/686 (77.26%), Query Frame = 0

Query: 242 EEVSLNKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSWT 301
           E+V L K+LAY +DV FS++PYAK LALL+AT++LI+ GGLAL+ V+D  + + LWLSWT
Sbjct: 173 EDVPLKKRLAYSLDVCFSVYPYAKLLALLLATVVLIVYGGLALYAVSDCGVDEALWLSWT 232

Query: 302 YVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQN 361
           +VADSG+HA+  G+G R+VSV+IS GGMLIFA MLGL+SD+IS+  DSLRKG+SEV+E N
Sbjct: 233 FVADSGSHADRVGVGARIVSVAISAGGMLIFATMLGLISDAISKMVDSLRKGKSEVLESN 292

Query: 362 HTLILGWSDKLGSLLHQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICR 421
           H LILGWSDKLGSLL QLAIAN+S+GGG VVV+AERDKEEME DIAK EFD  GTSVICR
Sbjct: 293 HILILGWSDKLGSLLKQLAIANKSIGGGVVVVLAERDKEEMETDIAKFEFDLMGTSVICR 352

Query: 422 SGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVELS 481
           SGSPLILADLKKVSVS ARAIIV+  D NADQSDARALR VLSLTGV+EG +GH+VVE+ 
Sbjct: 353 SGSPLILADLKKVSVSNARAIIVLGSDENADQSDARALRVVLSLTGVKEGWKGHVVVEMC 412

Query: 482 DLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQ 541
           DLDNE LVKLVGGE +ETVVAHDVIGRLMIQCA QPGLAQIWEDILGFEN EFYIK+WPQ
Sbjct: 413 DLDNEPLVKLVGGERIETVVAHDVIGRLMIQCALQPGLAQIWEDILGFENAEFYIKKWPQ 472

Query: 542 LDGMQFLDVLISFPDAIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAA 601
           LDG  F DVLISFP+AIPCG+KVA+  GKIVLNP D YVL+EGDE+LVIAEDDDTYAP +
Sbjct: 473 LDGYCFEDVLISFPNAIPCGVKVAA-DGKIVLNPSDDYVLKEGDEILVIAEDDDTYAPGS 532

Query: 602 LSTVREASSMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKSAERILLCGW 661
           L  VR                  +C                 PK  + PK  E+IL CGW
Sbjct: 533 LPEVR------------------MC---------------HFPKMQDPPKYPEKILFCGW 592

Query: 662 RRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAVI 721
           RRD++DMI VL+A LAPGSELWMFN+VP+ EREKKLTD GL+IS+L NI LV R+GNAVI
Sbjct: 593 RRDIDDMIKVLEALLAPGSELWMFNEVPDQEREKKLTDAGLNISKLVNIKLVHRQGNAVI 652

Query: 722 RRHLESLPLESFDS-------------------------------AKRLPVRDAKTTAHR 781
           RRHLESLPLE+FDS                               +KRLP +DAK++A R
Sbjct: 653 RRHLESLPLETFDSILILAEQSLENSIVHSDSRSLATLLLIRDIQSKRLPYKDAKSSALR 712

Query: 782 -GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAED 841
              F    WI +MQQASDKS++ISEILD RTKNL+S+S+ISDYVLSNELVSMALAMVAED
Sbjct: 713 ISGFPNCCWIRKMQQASDKSIVISEILDSRTKNLVSVSRISDYVLSNELVSMALAMVAED 772

Query: 842 RQINDVLEELFAEEGNELHIRHADLYLREGEELSFYEVLLRARRRREVVIGYRSANAERA 896
           +QIN VL+ELFAE+GNEL IR A+ Y+ + EE+ FY+++ RAR+R+E++IGYR A  E+A
Sbjct: 773 KQINRVLKELFAEKGNELCIRPAEFYIYDQEEVCFYDIMRRARQRQEIIIGYRLAGMEQA 824

BLAST of Sed0015965 vs. TAIR 10
Match: AT5G43745.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 218.0 bits (554), Expect = 3.1e-56
Identity = 179/669 (26.76%), Postives = 327/669 (48.88%), Query Frame = 0

Query: 267 LALLIATLLLIMLGGLALFGVTDD-SLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSIS 326
           +AL+IA +  +++GGL  F    D  L DCLW +W  +  S  H   +    R++   ++
Sbjct: 167 VALMIACVSFVIIGGLLFFKFRKDLPLEDCLWEAWACLISSSTHLKQKTRIERVIGFVLA 226

Query: 327 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQNHTLILGWSDKLGSLLHQLAIANE 386
             G+L ++ +L  +++        LR+G + +V+E +H +I G +  L  +L QL   +E
Sbjct: 227 IWGILFYSRLLSTMTEQFRYNMTKLREGAQMQVLEADHIIICGINSHLPFILKQLNSYHE 286

Query: 387 ---SLGGGT-----VVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKVSV 446
               LG  T     +++M++  +++M+        DF    ++ +S S  +    ++ + 
Sbjct: 287 HAVRLGTATARKQRLLLMSDTPRKQMDKLAEAYSKDFNHIDILTKSCSLNLTKSFERAAA 346

Query: 447 SKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVELSDLDNEVLVKLVGGEL 506
           S ARAII++   G+  + D  A  +VL+L  +++      +VE+S  +   L+K + G  
Sbjct: 347 SMARAIIILPTKGDRYEVDTDAFLSVLALQPIQKMESIPTIVEVSSPNTYDLLKSISGLK 406

Query: 507 VETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFLDVLISFPD 566
           VE V   +V  +L +QC+RQ  L +I+  +L +    F +  +P L G ++  + + F +
Sbjct: 407 VEPV--ENVTSKLFVQCSRQKDLIKIYRHLLNYSKNVFNLCSFPNLVGTKYRQLRLGFQE 466

Query: 567 AIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALSTVREASSMHIARP 626
            + CG+      GK+  +P D+  L E D++L IA   +      L T  +  ++ +   
Sbjct: 467 VVVCGL---LRDGKVNFHPNDNEELMETDKLLFIA-PLNWKKKQLLYTDMKLENITVPTD 526

Query: 627 TRKPQKILLCGWRRDIDDMIVVWRGSLPKDFNVPKS-AERILLCGWRRDMEDMIMVLDAF 686
           TRK    +    R  +  +I+  R SL K  +  K   E ILL GWR D+  MI   D +
Sbjct: 527 TRKQ---VFEKKRSRLSKIIMRPRKSLSKGSDSVKGPTESILLLGWRGDVVQMIEEFDNY 586

Query: 687 LAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS 746
           L PGS + + +DV   +R +      +   +++NI +  + GN          PL ++D+
Sbjct: 587 LGPGSSMEILSDVSLEDRRR--VGDSIGSVKIKNIQVSHKVGN----------PL-NYDT 646

Query: 747 AKRLPVRDAKTTAHRGS--------FSQGSW-IGEMQQASDKSV---------------- 806
            K+  +R  K+   +G          S   W +G+  +A  +S                 
Sbjct: 647 LKQTIMR-MKSKYRKGKNIPLTILVISDRDWLLGDPSRADKQSAYSLLLAESICNKLGVK 706

Query: 807 ---IISEILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNE 866
              + SEI+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   +G+E
Sbjct: 707 VHNLASEIVDSKLGKQITGLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDADGDE 766

Query: 867 LHIRHADLYLREGEELSFYEVLLRARRRREVVIGYRSANAERAVINPPAKNERRKWSLRD 896
           ++++  +LY++EGE  SF E+  RA  RREV IGY      + +INP  KNE     + D
Sbjct: 767 IYVKDVELYMKEGENPSFTELSERAWLRREVAIGY--IKGGKKMINPVPKNEPLSLEMDD 810

BLAST of Sed0015965 vs. TAIR 10
Match: AT5G02940.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 210.7 bits (535), Expect = 4.9e-54
Identity = 172/662 (25.98%), Postives = 319/662 (48.19%), Query Frame = 0

Query: 267 LALLIATLLLIMLGGLALFGV-TDDSLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSIS 326
           + LLI     +++GGL  F    D SL DCLW +W  + ++  H   +    RL+   ++
Sbjct: 163 VVLLITCFSFVIIGGLFFFKFRKDTSLEDCLWEAWACLVNADTHLEQKTRFERLIGFVLA 222

Query: 327 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQNHTLILGWSDKLGSLL-------- 386
             G++ ++ +L  +++        +R+G   +V+E +H +I G +  L  +L        
Sbjct: 223 IWGIVFYSRLLSTMTEQFRYHMKKVREGAHMQVLESDHIIICGINSHLPFILKQLNSYQQ 282

Query: 387 HQLAIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVICRSGSPLILADLKKVSV 446
           H + +   +    T+++M++  ++EM+        DF    ++ +S S  +    ++ + 
Sbjct: 283 HAVRLGTTTARKQTLLLMSDTPRKEMDKLAEAYAKDFDQLDILTKSCSLNMTKSFERAAA 342

Query: 447 SKARAIIVIAEDGNADQSDARALRTVLSLTGVREGLRGHIVVELSDLDNEVLVKLVGGEL 506
             ARAII++   G+  + D  A  +VL+L  +++      +VE+S  +   L+K + G  
Sbjct: 343 CMARAIIILPTKGDRYEVDTDAFLSVLALEPIQKMESIPTIVEVSSSNMYDLLKSISGLK 402

Query: 507 VETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFLDVLISFPD 566
           VE V   +   +L +QC+RQ  L +I+  +L +    F +  +P L GM++  + + F +
Sbjct: 403 VEPV--ENSTSKLFVQCSRQKDLIKIYRHLLNYSKNVFNLCSFPNLTGMKYRQLRLGFQE 462

Query: 567 AIPCGIKVASLGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALSTVREASSMHIARP 626
            + CGI      GK+  +P D   L E D++L IA     +    L T  +  +M +   
Sbjct: 463 VVVCGI---LRDGKVNFHPNDDEELMETDKLLFIAPLKKDF----LYTDMKTENMTVDE- 522

Query: 627 TRKPQKILLCGWRRDIDDMIVVWRGSLPK---DFNVPKSAERILLCGWRRDMEDMIMVLD 686
           T   +K +    +  ++ +I     SL K    F  PK  E ILL GWR D+ +MI   D
Sbjct: 523 TDDTRKQVYEEKKSRLEKIITRPSKSLSKGSDSFKGPK--ESILLLGWRGDVVNMIKEFD 582

Query: 687 AFLAPGSELWMFNDVPENEREKKLTDGGLDISRLENISLVDREGNAVIRRHLESLPLESF 746
           ++L PGS L + +DVP  +R  +  D  +   +++NI +    GN +    L+   +   
Sbjct: 583 SYLGPGSSLEILSDVPLEDR--RGVDQSIATGKIKNIQVSHSVGNHMDYDTLKESIMHMQ 642

Query: 747 DSAKRLPVRDAKTTAHRGSFSQGSWIGEMQQASDKSV-------------------IISE 806
           +  ++    D + T    S  +   +G+  +A  +S                    + SE
Sbjct: 643 NKYEK-GEEDIRLTIVVIS-DRDLLLGDPSRADKQSAYTLLLAETICNKLGVKVHNLASE 702

Query: 807 ILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRHAD 866
           I+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   EG+E++++  +
Sbjct: 703 IVDTKLGKQITRLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDAEGDEIYVKDIE 762

Query: 867 LYLREGEELSFYEVLLRARRRREVVIGYRSANAERAVINPPAKNERRKWSLRDDFVVIAE 896
           LY++EGE  SF E+  RA  RREV IGY      + +INP  K E     + D  +VI+E
Sbjct: 763 LYMKEGENPSFTELSERAWLRREVAIGY--IKGGKKIINPVPKTEPVSLEMEDSLIVISE 806