Sed0015960 (gene) Chayote v1

Overview
NameSed0015960
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontRNA (Adenine(37)-N6)-methyltransferase
LocationLG08: 3486030 .. 3491257 (-)
RNA-Seq ExpressionSed0015960
SyntenySed0015960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAGCTGGCGGATCGAGTTCGCCTGCGCGCCGAGCTGCGTTCAAATCTCAACTCTGAAACCTAATAATGGCCGACGGCGCAGAATCAACCTGGCCGCCGGCCGCCATGGGCGTCACACTGGCGATCATCTCCGCCTCCGCTATCATATCTCGTAAAACCTCGAAACTTTTCAAGATCCTCGAACTCATCTCTCTCAATTTCTCTCATGTGAAGTTTTGCGTTTTTTCTCAGTGTATGTAGCGACGAGGAGATCGAGAGCGTTGGATTCCAAGGTACGAGAGCTCGAGGCATCTCTGACAGCTTCGTCAAAGAACACCGCCGCCGAGCGAAAAGGCCGAATTCGGGCTCAGCAGGTATTTCATTATCTCCTCCTCTCCATGGCTTCATGTTCCAATGCTATTAACACTGTTTTTAAAAAGCTCCCACTAGAGGCCTCTCACGCTCAAAAAGGTTCCGGGCTCAATGTTGGCGGATAAAAAATCCCATGAAAGGCTTGCCAGTCCGGTGCCTTGTGGGGTTTTACTAGGTTTTCTCTATTTATTTTGTAAATTTCAAATTCAGTCATCAATTGCTTTGAAGTTCTATTTAATAAAAACAATTAAAACTTAATTGTCAGTCTTAATTACCAATCCACATCTTTGTTCTCTCTACCTATTGTTAATGAAGGGTCCCCAAGGAGTAGCACAGTGGTTGAAGACTTGGACTTTGAGGGTATCCTCCCCTCAAGATCCTAGGTTCGAGACTCAGCTGTGACATTACTCCTTCGATGTCTCCCAGTGCCCGGCCTAGGGACAAGCATGGGTACCCTTGTTTCAAAAAAAAAAAAATGTTAATGAAGGAGTGGATGGGATGAATATGCATTTAGCTACCATAAATTGATCTTTAGGATTTTAAATTTTTATTTGGGATTAATGTTTGAATGTTTCTTGTTTTTTTTGAAACAAGGGTAATCACATCCGTCAAAGCACTGGATACATCGAAGGAATAATGTTTCATGTTTTTTTATGGAGGGACTATATAAACAAATTTATATTGAAAGAGATTAATATATATATTTGTATGTATACATGTATATATTCTGATAAAAAAAAGGTCATTGAGCTTTAGTGAGCTTGAGCCTTTTAAGACACTGGCTATTCATGATAGTTTGATTACTTATTTCTCCATATTTGTAGGCTTTAAGGCAAGCACTTGCGGAACCGAAGTCTGATAGCGTGGAGTGCACTTCATACCCTATGACACCAATTGGTGTCATACACTCATCCTTCTCTACTAGGTTAGTTTAAGCTTGCATTATCCGGCAGTCCAGTGTTTTAAAAGGCGTGCTTGGGCTCAAGTCCCACCTTTTGCGTCTCGCATGTCCCAGGCGAGGCGCGTGTAATAAGGCGCACTCCTTATGGCGCCTTACGTGAAGCCCTGGGCGCCAAAAGACGCGCGCCTCGTGGGCTTTTTTATTTTCCTTTGCATTTTTTGTATTTCAGTTCCCCGTACTAGGTCATTTGAAGCGCGTGAAGGTTGTTAGTTGAATATTATGTATGTTTTATATATTATTCTTGGTTAAAACTTCATTTATGTCCATGTAAAGTGTTTTTATTGTGTATACACACGTATATATAAATATTTTGTGTTGTTTTATATAGTGCGCCTTAAGTAAAAAAGTCCTCGCCTTTTGCCTTTTGGTGTGCCTCAGGCTCTCGAGGGTCATTTCACCTTAGTGTGCCTTGCGCTTTTTAAAACACTGCAGCAGTCTAACATCAATTTTTGAATTGTTGTTTAGTTTTCTCGTTGTTCTGTTTGAACACCAATTATAAAATAATATGGGATTCCATTTCAAAACAAATTGTTGATGATGAGAGTAGCCCACATATTTTATAAAAATGTTGAGAACTCTTCACCTTTTCAATGCGAGAATCTCCAACATACTCCTTAAGATGGTGGGAACACCATCTTTTATTAGCTTCGAATTTGTGGATCAATACCCGCTTAGAATTTAATCGTTTAGGTTGGATCTAAATTTTTGGATCAAATACCTACTTAGGTTGGATTTCGAATTTATGGACCAAATAATCACTTGGAACTCTGATTTCATATTAGATTACATGGGGTTTCATCTCAAAACCAATTGATGATGATAGGAGTAGTTCACATATCTTATAAAGATGCTGAGAACTCCTTATCTTTTCAAATGTGGGACAAGGTGAGGATCTCCAACATAAATAGATATGTTTCAGCCTTGTTCTTTGTTAGGTATAGAAGTGGAACAATCAACGACTTTTGTTGGGTTATTTTAGGAATGGGACTCCAAGGCAATCTTTAGTAGTACCCCTTGCGAGGGCACGACTGGTGTTATCATCTCGTATCCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAATACAAATCTTGAGAAGTCGTGGAAGGATCCTAGATCAAAATTCAAGGCAAAAGTACGTTTCTTCATCTGTTGATGCTCCTTAAAAATGGAATTCATAGTTTTATTATTTTTATAGTTTCTTAAAATCCTATAAAAGAACAGCTCATTGATAGGTTAAAGTACCGAGGTTGAAAGGCGCAAAGCTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACCATTGCAAAGGTAAGATATAAATTTCAATTGTTTTCAATGTATTATTGCAAGTTGAGGCAGACGCCACATACATTCATATAAATGTCACCCCCAACAAATTTTACCCACTTGACCAGATTGACATGGTCCAATCACACCAATGATTTTTCTACGGTTTTGCCTTTTTCATTGTCATTTACTATTATAAATCTATAGCTACAAGCTTAGGGGGTGTTTAGGGCCCGGGGCATAGAGGACGAGAATGATTATGGGTGGAATCGTTTTATTTGCCTTGTTTGGGCGAATAAGAATCATTTCTTCAAAAATACTTTTTATTCTATTCAAACCACTTTTCATTCTTATGGTACTCGTACTCACTATTTTATTCTCTCTATAACTTTTTTCTTCACCCTTAATATAAGTAATGGTTTCTCCCATAATCATTTTCTTTCCCTCAGACACATAATTATTCTATCTCATATTCTCCTCCTAATTCTTATTCTTCCCATAATCATTCTCATTCTCTTGCCCTAAACACCCCCAGGCCCCATAAAGTTGTACTGTTAGATAACATTCAATGAAATATATAAATGGGGGATTCTTAAATCTAAGACTAAGAAATCTGCTATGTTACCACTTTAGGTGGAGGCTATACAGGGACATCAAATTGTACTCTCTGGTGCCGATCTTGTTGATGGAACTGTTAGTGTTTTTGTCTCTCTTTTTTTTTCCCAATCAGCAGTGTAAGAGATTTAACTTGAAATTAATCATTACTTTTTGTGATCATGATTGTTATGATTTTTTTCTCTGTTTGAATTTTGTTTCAGCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTCTCCAAGATGCAAAAGTTCCAGAGTGGATAATGGTATTTGTTCAATATTTCTATTAGAATCTCTTTGATGTTTATTATTACCCTGTTCACCCTTTCAATTATTTTTCATGTAGTATTTTTTAACTACTCATTTTCCTGTCTCAACTTAGATTTATTTTGTTTTCAAATTTTAAAATGCGGAAAATCTCTAATATTCTTTGCATGCCATTTAACTAGTTGCGTGCAAATAACTTAAGAAATTTAGCTTTCTATATATGATGCTGTTTTTTTCTTTATAGTTGAACTTATCTATACTCTATGATCATGTAATATGTATTATACATTCTTCGTGGCAATCTGACATCTTATATTACGCAAACCCAGGGGGAAAATTTGTTGAGCGTTGCATCTATTTGCTACTCTGAAGAATTTACTTCCACTTTAATGGAATGTTGGCCACTAATGGTGAGTTTTGTTTTGTTTCTTTTTTCTCTCTTTATTTCTATTTAAAAGTGGTTCTGTTCTTTTTCATCTCTGATTTGAAGTTTAGCTTGTTCATGCAATATGCCAAAAATATTCACTATTTCTTTTGCACACGAAAGTAATAAACAGTTTTTTTTTAAACAAGGGTAAACATGTCCGTCCCTTGGCCAGGCACCGGGAGACATCGAAAGAATAATGTCACAACTGAGTCTCGAACCTGGGACCTTGAGGGGAGTATACCCTCAAAGCCTAAGTCTTCGACCACTGTGCCACCCCTTGAGGACAGGAAAGTAATAAACAGTAAACAACAATATTCTACCTAGCACTTCTAAGTTCCAAACTATTATATTTATGGCCTATGGAACGTCCTTACTGTTTAGTGCTTTGAGTTTGGGTGATAATTTGAGTTTGGTTGGAACCTCAAATTTAAAGTTTGATTAATAAAAGTGATAATTATTGTTGTCTCCCGGTGATGTTGTTTCACGCAAACTCTAAATGCATATGAACATTGATCTGGTTAATTTCTTATTCTTTTACTCCATTATAGTACTTTAAAGAGCTGCTTTGAAATTCGATTATCATTTTTCTAACTCCACATTACAAAGTTCTAGTGCTCTCAATTTTCATACTGTAAAAAGCTGGATATTCCGTGTCAAGTATGTAGGATCCCGTCCTTGCTACATAAATTTCACTTGTTATCATATTTTTGTTGTTTCAGGAAAAGGAATCACTTTATGAATCGGCAGATGAGTTGAAAAAGTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCTCAACGTAGTCGACCTCACGACTCCCACATTACGACAACAACAGGGCATGAATTGTCCGATGGAAGTTCAGATTCAGATGGTCACCTAGATGACCAAGTTTCAAGTGGTACCCAAGTTTCTTCTAAGAACATTATCTATCACTTACTTGTAGATGGATTAGATGTGTCGTATACGATCGATTGCCATGGTGACGTGGTTGTGGGCAGAGTAGCACTCTCATCACCAAGCTCAAGAACAAGAAGTGATCGACACAAACCCAACTCTTCTGGATAGGCAAATCAAGGTAACTTAGTCTTTGTATTGTACTAACTTTGCACCACAGTTAAAAGAACCTCTATGTAGGGAATTTTCACCCAAAAATACTATTCTGCCTTACATGGAGCTCGATATGCTTACATTCCTGTTCTCTTGAAAAAAGCTGTATGGTTTGAGTCTGGGACTATAATTCTG

mRNA sequence

TGAAGCTGGCGGATCGAGTTCGCCTGCGCGCCGAGCTGCGTTCAAATCTCAACTCTGAAACCTAATAATGGCCGACGGCGCAGAATCAACCTGGCCGCCGGCCGCCATGGGCGTCACACTGGCGATCATCTCCGCCTCCGCTATCATATCTCTGTATGTAGCGACGAGGAGATCGAGAGCGTTGGATTCCAAGGTACGAGAGCTCGAGGCATCTCTGACAGCTTCGTCAAAGAACACCGCCGCCGAGCGAAAAGGCCGAATTCGGGCTCAGCAGGCTTTAAGGCAAGCACTTGCGGAACCGAAGTCTGATAGCGTGGAGTGCACTTCATACCCTATGACACCAATTGGTGTCATACACTCATCCTTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTAGTAGTACCCCTTGCGAGGGCACGACTGGTGTTATCATCTCGTATCCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAATACAAATCTTGAGAAGTCGTGGAAGGATCCTAGATCAAAATTCAAGGCAAAAGTTAAAGTACCGAGGTTGAAAGGCGCAAAGCTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACCATTGCAAAGGTGGAGGCTATACAGGGACATCAAATTGTACTCTCTGGTGCCGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTCTCCAAGATGCAAAAGTTCCAGAGTGGATAATGGGGGAAAATTTGTTGAGCGTTGCATCTATTTGCTACTCTGAAGAATTTACTTCCACTTTAATGGAATGTTGGCCACTAATGGAAAAGGAATCACTTTATGAATCGGCAGATGAGTTGAAAAAGTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCTCAACGTAGTCGACCTCACGACTCCCACATTACGACAACAACAGGGCATGAATTGTCCGATGGAAGTTCAGATTCAGATGGTCACCTAGATGACCAAGTTTCAAGTGGTACCCAAGTTTCTTCTAAGAACATTATCTATCACTTACTTGTAGATGGATTAGATGTGTCGTATACGATCGATTGCCATGGTGACGTGGTTGTGGGCAGAGTAGCACTCTCATCACCAAGCTCAAGAACAAGAAGTGATCGACACAAACCCAACTCTTCTGGATAGGCAAATCAAGGTAACTTAGTCTTTGTATTGTACTAACTTTGCACCACAGTTAAAAGAACCTCTATGTAGGGAATTTTCACCCAAAAATACTATTCTGCCTTACATGGAGCTCGATATGCTTACATTCCTGTTCTCTTGAAAAAAGCTGTATGGTTTGAGTCTGGGACTATAATTCTG

Coding sequence (CDS)

ATGGCCGACGGCGCAGAATCAACCTGGCCGCCGGCCGCCATGGGCGTCACACTGGCGATCATCTCCGCCTCCGCTATCATATCTCTGTATGTAGCGACGAGGAGATCGAGAGCGTTGGATTCCAAGGTACGAGAGCTCGAGGCATCTCTGACAGCTTCGTCAAAGAACACCGCCGCCGAGCGAAAAGGCCGAATTCGGGCTCAGCAGGCTTTAAGGCAAGCACTTGCGGAACCGAAGTCTGATAGCGTGGAGTGCACTTCATACCCTATGACACCAATTGGTGTCATACACTCATCCTTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTAGTAGTACCCCTTGCGAGGGCACGACTGGTGTTATCATCTCGTATCCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAATACAAATCTTGAGAAGTCGTGGAAGGATCCTAGATCAAAATTCAAGGCAAAAGTTAAAGTACCGAGGTTGAAAGGCGCAAAGCTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACCATTGCAAAGGTGGAGGCTATACAGGGACATCAAATTGTACTCTCTGGTGCCGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTCTCCAAGATGCAAAAGTTCCAGAGTGGATAATGGGGGAAAATTTGTTGAGCGTTGCATCTATTTGCTACTCTGAAGAATTTACTTCCACTTTAATGGAATGTTGGCCACTAATGGAAAAGGAATCACTTTATGAATCGGCAGATGAGTTGAAAAAGTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCTCAACGTAGTCGACCTCACGACTCCCACATTACGACAACAACAGGGCATGAATTGTCCGATGGAAGTTCAGATTCAGATGGTCACCTAGATGACCAAGTTTCAAGTGGTACCCAAGTTTCTTCTAAGAACATTATCTATCACTTACTTGTAGATGGATTAGATGTGTCGTATACGATCGATTGCCATGGTGACGTGGTTGTGGGCAGAGTAGCACTCTCATCACCAAGCTCAAGAACAAGAAGTGATCGACACAAACCCAACTCTTCTGGATAG

Protein sequence

MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAERKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDPRSKFKAKVKVPRLKGAKLGVFATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPEWIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQRSRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCHGDVVVGRVALSSPSSRTRSDRHKPNSSG
Homology
BLAST of Sed0015960 vs. NCBI nr
Match: XP_022134721.1 (uncharacterized protein LOC111006924 [Momordica charantia])

HSP 1 Score: 617.1 bits (1590), Expect = 1.0e-172
Identity = 312/380 (82.11%), Postives = 345/380 (90.79%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA GAES W  AAM + LA ISASAI+SLY+ TR+SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+ALA+PKSD+VECTSYPMTPIGVI S+FSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGLGEYSHCWIIYVFHLNT+L+K WK+P +S+FKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VPE
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W+MG+NLLSVASIC+SEEFTSTL +CWPLMEK+S Y SADE K FVKQVLSWDIRSLSQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           +RPH+SHI TTTGHELSDGSSD D HL D+ S GTQ+SSKNIIYHLLVDGL+VSYTI CH
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           GDVVV +  L+SPSSRTRS+
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

BLAST of Sed0015960 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 5.8e-171
Identity = 305/380 (80.26%), Postives = 346/380 (91.05%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  AES W  AA+ VTLA ISASA+ISLYV T++SR L +KVRELEASL +S K TAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A+AEPKSDSVECTSYPMTPIG I SSFSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGLGEYSHCW+IYVFHLNT+L+K WKDP +SKFKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W++G+NLL+VASIC+SEEF  TL ECWPLMEK+SLY S DE + FVK+VLSWDIRS+SQR
Sbjct: 241 WLLGDNLLTVASICFSEEFIPTLSECWPLMEKKSLYASEDEFRNFVKEVLSWDIRSVSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           ++PHDSH+  TTGHE  DGSSDS+GHLDD+VSSG+Q SS+NIIYHLLVDGLDVSYTI CH
Sbjct: 301 NQPHDSHVKPTTGHECFDGSSDSEGHLDDKVSSGSQSSSENIIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           G+VVVG+V L+SPSSRTRS+
Sbjct: 361 GNVVVGKVELASPSSRTRSN 380

BLAST of Sed0015960 vs. NCBI nr
Match: KAG7023410.1 (tRNA (adenine(37)-N6)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 590.5 bits (1521), Expect = 1.1e-164
Identity = 301/375 (80.27%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  A S    AAM VTLA +SASAI+SLY  T++SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A++EPKSDSVECT YPM PIG I SSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V SSRIPPASIEGLGEYSHCW+IYVFHLNTNL+K WKDP +SKFKAKVKVPRLKG K+G+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIG+TIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           WI GEN+LSVASIC+SEEFT +L+ECWPL+EK+SLY SADELK FVKQVLSWDIRSLSQR
Sbjct: 241 WITGENILSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGT-QVSSKNIIYHLLVDGLDVSYTIDC 360
           S+PH+S++TTT GHELSDGSSDSDGHLD+ + SGT Q SSK ++YHLLVDGL+VSYTI C
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 HGDVVVGRVALSSPS 374
            GDVVV +V L+SPS
Sbjct: 361 QGDVVVRKVELASPS 375

BLAST of Sed0015960 vs. NCBI nr
Match: XP_022965024.1 (uncharacterized protein LOC111464972 [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.4e-164
Identity = 301/375 (80.27%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  A S    AAM VTLA +SASAI+SLY  T++SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A++EPKSDSVECT YPM PIG I SSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V SSRIPPASIEGLGEYSHCW+IYVFHLNTNL+K WKDP +SKFKAKVKVPRLKG K+G+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIG+TIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           WI GEN+LSVASIC+SEEFT +L+ECWPL+EK+SLY SADELK FVKQVLSWDIRSLSQR
Sbjct: 241 WITGENVLSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGT-QVSSKNIIYHLLVDGLDVSYTIDC 360
           S+PH+S++TTT GHELSDGSSDSDGHLD+ + SGT Q SSK ++YHLLVDGL+VSYTI C
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 HGDVVVGRVALSSPS 374
            GDVVV +V L+SPS
Sbjct: 361 QGDVVVRKVELASPS 375

BLAST of Sed0015960 vs. NCBI nr
Match: TYK09463.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 296/380 (77.89%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  AES W  AAM V L  ISASAIISLY+ T++SR L +KV++LEASL AS K TAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A+AEPKSDSVECTSYPMTPIG I SSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGL EYSHCW+IYVFHLNT+L+K WKDP +SKFKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+I+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W++G+N+L+VASIC+SEEFTS L +CWPL+EK+SLY S +ELK FVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           SRPH SHI T TG EL D SSDSDGH+DD+VSS +Q SSK+ IYHLLVDGLDVSYTI  H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           GDVVV +V L+SPSSRTRS+
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

BLAST of Sed0015960 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 1.2e-33
Identity = 75/150 (50.00%), Postives = 98/150 (65.33%), Query Frame = 0

Query: 92  PIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRI---PPASIEGLGEYSHCWIIYVFHL 151
           P+G + S FS +NGTPRQ  +   +RA L +  RI   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTNLEKSWKDPRSKFKAKVKVPRLKGAKLGVFATRSPHRPCPIGLTIAKVEAIQGHQIVL 211
           N +L           KAKV+ PRL GAK GVF+TRSPHRP  IGLT+AK+E ++G  I L
Sbjct: 92  NGHL---------SCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIYL 151

Query: 212 SGADLVDGTPVLDVKPYLPYCDSLQDAKVP 239
           SG D++ GTPVLD+KPY+   DS Q+   P
Sbjct: 152 SGIDMIHGTPVLDIKPYIAEYDSPQNVMEP 172

BLAST of Sed0015960 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 5.8e-33
Identity = 74/149 (49.66%), Postives = 97/149 (65.10%), Query Frame = 0

Query: 92  PIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRI---PPASIEGLGEYSHCWIIYVFHL 151
           PIG + S F  + GTPRQ  +   +RA L +   I   P  S+ GL E+SH WI++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 152 NTNLEKSWKDPRSKFKAKVKVPRLKGAKLGVFATRSPHRPCPIGLTIAKVEAIQGHQIVL 211
           N +L          +KAKV+ PRL GAK GVF+TRSPHRP  IGLT+AK+E ++G  + L
Sbjct: 92  NGHL---------NYKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVYL 151

Query: 212 SGADLVDGTPVLDVKPYLPYCDSLQDAKV 238
           SG D++DGTPVLD+KPY+   DS Q+  V
Sbjct: 152 SGVDMIDGTPVLDIKPYIADYDSPQNLSV 171

BLAST of Sed0015960 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 8.4e-32
Identity = 72/146 (49.32%), Postives = 96/146 (65.75%), Query Frame = 0

Query: 92  PIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRI---PPASIEGLGEYSHCWIIYVFHL 151
           PIG + S FS + GTPRQ  +   +RA L +   I   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTNLEKSWKDPRSKFKAKVKVPRLKGAKLGVFATRSPHRPCPIGLTIAKVEAIQGHQIVL 211
           N +L          +KAKV+ PRL GAK GVF+TRSPHRP  IGLT+AK+E ++G  + L
Sbjct: 92  NGHL---------NYKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVYL 151

Query: 212 SGADLVDGTPVLDVKPYLPYCDSLQD 235
           SG D++ GTPVLD+KPY+   DS Q+
Sbjct: 152 SGIDMIHGTPVLDIKPYIADYDSPQN 168

BLAST of Sed0015960 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 65/155 (41.94%), Postives = 97/155 (62.58%), Query Frame = 0

Query: 87  SYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRIPP----ASIEGLGEYSHCWI 146
           ++ ++PIG I S F  +   PRQ L+ P AR  L L   +PP     ++EGL + SH W+
Sbjct: 2   THSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTLEL---LPPFDQVEALEGLEQVSHVWL 61

Query: 147 IYVFHLNTNLEKSWKDPRSKFKAKVKVPRLKGAK-LGVFATRSPHRPCPIGLTIAKVEAI 206
           +++FH         +    K + KV+ PRL G + LGVFATR+ HRP  IG ++ ++E  
Sbjct: 62  LFLFH---------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEGF 121

Query: 207 QGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAK 237
           +  ++ LSG DL+DGTPVLD+KPY+PY D++ DA+
Sbjct: 122 EAGRLWLSGIDLLDGTPVLDIKPYVPYADAVADAR 144

BLAST of Sed0015960 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.9e-24
Identity = 62/154 (40.26%), Postives = 93/154 (60.39%), Query Frame = 0

Query: 87  SYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARLVLSSRIPP----ASIEGLGEYSHCWI 146
           S+ ++PIG + S F  +   PRQ  + P A   L L   +PP     ++EGL + SH W+
Sbjct: 2   SHSISPIGHVRSCFKEKFAIPRQPQLAPAATGVLEL---LPPFDTGDAVEGLEQVSHVWL 61

Query: 147 IYVFHLNTNLEKSWKDPRSKFKAKVKVPRLKG-AKLGVFATRSPHRPCPIGLTIAKVEAI 206
           I++FH         +    K + KV+ PRL G   +GVFATR+ HRP  +G ++ K+E +
Sbjct: 62  IFLFH---------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEKV 121

Query: 207 QGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDA 236
           +  ++ LSG DL+DGTPV+D+KPY+PY D + DA
Sbjct: 122 EPGRLWLSGIDLLDGTPVIDIKPYVPYADIVPDA 143

BLAST of Sed0015960 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 5.1e-173
Identity = 312/380 (82.11%), Postives = 345/380 (90.79%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA GAES W  AAM + LA ISASAI+SLY+ TR+SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+ALA+PKSD+VECTSYPMTPIGVI S+FSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGLGEYSHCWIIYVFHLNT+L+K WK+P +S+FKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VPE
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W+MG+NLLSVASIC+SEEFTSTL +CWPLMEK+S Y SADE K FVKQVLSWDIRSLSQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           +RPH+SHI TTTGHELSDGSSD D HL D+ S GTQ+SSKNIIYHLLVDGL+VSYTI CH
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           GDVVV +  L+SPSSRTRS+
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

BLAST of Sed0015960 vs. ExPASy TrEMBL
Match: A0A6J1HPV8 (uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC111464972 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.7e-165
Identity = 301/375 (80.27%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  A S    AAM VTLA +SASAI+SLY  T++SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A++EPKSDSVECT YPM PIG I SSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V SSRIPPASIEGLGEYSHCW+IYVFHLNTNL+K WKDP +SKFKAKVKVPRLKG K+G+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIG+TIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           WI GEN+LSVASIC+SEEFT +L+ECWPL+EK+SLY SADELK FVKQVLSWDIRSLSQR
Sbjct: 241 WITGENVLSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGT-QVSSKNIIYHLLVDGLDVSYTIDC 360
           S+PH+S++TTT GHELSDGSSDSDGHLD+ + SGT Q SSK ++YHLLVDGL+VSYTI C
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 HGDVVVGRVALSSPS 374
            GDVVV +V L+SPS
Sbjct: 361 QGDVVVRKVELASPS 375

BLAST of Sed0015960 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.6e-164
Identity = 296/380 (77.89%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  AES W  AAM V L  ISASAIISLY+ T++SR L +KV++LEASL AS K TAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A+AEPKSDSVECTSYPMTPIG I SSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGL EYSHCW+IYVFHLNT+L+K WKDP +SKFKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+I+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W++G+N+L+VASIC+SEEFTS L +CWPL+EK+SLY S +ELK FVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           SRPH SHI T TG EL D SSDSDGH+DD+VSS +Q SSK+ IYHLLVDGLDVSYTI  H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           GDVVV +V L+SPSSRTRS+
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

BLAST of Sed0015960 vs. ExPASy TrEMBL
Match: A0A6J1JCZ9 (uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.4e-164
Identity = 297/375 (79.20%), Postives = 334/375 (89.07%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA    S    AAM +TLA +SASAI+SLY  T++SR L SKVRELEASL AS K T AE
Sbjct: 1   MAYAVVSRSSTAAMSITLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A++EPKSDSVECT YPM PIG I SSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V SSRIPPASIEGLGEYSHCW+IYVFHLNTNL+K WKDP +SKFKAKVKVPRLKG K+G+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIG+TIAKVEAIQGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W+ GEN+LSVASIC+SEEFT +L ECWPL+EK+SLY SADELK FVKQVLSWDIRSLSQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGT-QVSSKNIIYHLLVDGLDVSYTIDC 360
           S+PH+S++TTT GHELSDGSSDSDGHLD+ + SGT Q SSK+++YHLLVDGL+VSYTI C
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 HGDVVVGRVALSSPS 374
            GDVVV +V ++SPS
Sbjct: 361 QGDVVVRKVEVASPS 375

BLAST of Sed0015960 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.1e-162
Identity = 294/380 (77.37%), Postives = 338/380 (88.95%), Query Frame = 0

Query: 1   MADGAESTWPPAAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAE 60
           MA  AES W  AAM V L  ISASAIISLY+ T++SR L +KV++LEASL AS K TAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RKGRIRAQQALRQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARL 120
           R+GRIRAQQALR+A+AEPKSDSVECTSYPMTPIG I SSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VLSSRIPPASIEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGV 180
           V S+RIPPASIEGL EYSHCW+IYVFHLNT+L+K WKDP +SKFKAKV+VPRLKG ++GV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+I+LSGADLVDGTPVLDVKPYLPYCDS+QDA VP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WIMGENLLSVASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQR 300
           W++G+++L+VASIC+SEEFTS L +CWPL+EK+SLY S +ELK FVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 SRPHDSHITTTTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCH 360
           SRPH SHI T TG EL D SSDSDGH+DD+VSS +Q  SK+ IYHLLVDGLDVSYTI  H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVGRVALSSPSSRTRSD 380
           GDVVV +V L+SPSSRTRS+
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

BLAST of Sed0015960 vs. TAIR 10
Match: AT4G28020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0066 (InterPro:IPR001378); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 379.4 bits (973), Expect = 3.4e-105
Identity = 195/360 (54.17%), Postives = 259/360 (71.94%), Query Frame = 0

Query: 12  AAMGVTLAIISASAIISLYVATRRSRALDSKVRELEASLTASSKNTAAERKGRIRAQQAL 71
           AA+  TLA++S SA  SLY   RR   L+++++E E SL ++ + +A+ERKGR++AQQAL
Sbjct: 13  AALAFTLAVVSVSAAFSLYRWRRREEDLENRIKEFEKSLGSTLEKSASERKGRVKAQQAL 72

Query: 72  RQALAEPKSDSVECTSYPMTPIGVIHSSFSTRNGTPRQSLVVPLARARLVLS-SRIPPAS 131
           R+ALA+ +S  +  T YPM PIG I S FSTRNGTPRQ L+V LARA L+   + +PPAS
Sbjct: 73  REALAQTESHDLHSTIYPMRPIGTIQSCFSTRNGTPRQPLLVSLARACLIFDPALVPPAS 132

Query: 132 IEGLGEYSHCWIIYVFHLNTNLEKSWKDP-RSKFKAKVKVPRLKGAKLGVFATRSPHRPC 191
           +EGL EYSHCWI+YVFHLNT++EK W+ P +SK KAKV+VPRL G + GVFATRSPHRPC
Sbjct: 133 LEGLEEYSHCWILYVFHLNTDIEKLWRKPSQSKLKAKVRVPRLNGERKGVFATRSPHRPC 192

Query: 192 PIGLTIAKVEAIQGHQIVLSGADLVDGTPVLDVKPYLPYCDSLQDAKVPEWIMGENLLSV 251
           PIGLT+AKVE IQ  +++LSG DLVDGTPVLD+KPYLPY DS+Q A VP W+  +  L+V
Sbjct: 193 PIGLTVAKVEEIQKDKVLLSGVDLVDGTPVLDIKPYLPYSDSIQGASVPNWVKEDCSLAV 252

Query: 252 ASICYSEEFTSTLMECWPLMEKESLYESADELKKFVKQVLSWDIRSLSQRSRPHDSHITT 311
           AS+ +S+ F+S++  CW L+EK+SLY SADE +  + QVLSWDIRS+SQR++P D+    
Sbjct: 253 ASVTFSDTFSSSITSCWKLIEKKSLYSSADEFRSLITQVLSWDIRSMSQRNKPQDT---- 312

Query: 312 TTGHELSDGSSDSDGHLDDQVSSGTQVSSKNIIYHLLVDGLDVSYTIDCHGDVVVGRVAL 370
                           LD++           I+YHL+++GLDVSY ID   +++V  V+L
Sbjct: 313 ----------------LDEE-----------IVYHLVLEGLDVSYMIDNESNILVQDVSL 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134721.11.0e-17282.11uncharacterized protein LOC111006924 [Momordica charantia][more]
XP_038879626.15.8e-17180.26tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida][more]
KAG7023410.11.1e-16480.27tRNA (adenine(37)-N6)-methyltransferase [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022965024.11.4e-16480.27uncharacterized protein LOC111464972 [Cucurbita moschata][more]
TYK09463.11.2e-16377.89tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9BU701.2e-3350.00tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=... [more]
Q562D65.8e-3349.66tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV... [more]
Q4V7E08.4e-3249.32tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE... [more]
Q9RPT02.4e-2641.94Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae... [more]
O686384.9e-2440.26Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae... [more]
Match NameE-valueIdentityDescription
A0A6J1BYL55.1e-17382.11uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1HPV86.7e-16580.27uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC1114649... [more]
A0A5D3CC845.6e-16477.89tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1JCZ97.4e-16479.20uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678... [more]
A0A1S3BW921.1e-16277.37tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G28020.13.4e-10554.17unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0... [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036414YaeB, N-terminal domain superfamilyGENE3D2.40.30.70coord: 7..153
e-value: 7.2E-47
score: 160.8
IPR023370TrmO-like, N-terminal domainPFAMPF01980TrmOcoord: 15..140
e-value: 3.3E-42
score: 143.5
IPR023370TrmO-like, N-terminal domainPROSITEPS51668TSAA_2coord: 1..144
score: 56.233471
IPR023370TrmO-like, N-terminal domainCDDcd09281UPF0066coord: 3..135
e-value: 7.13632E-43
score: 141.475
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..242
NoneNo IPR availablePANTHERPTHR12818:SF0TRNA (ADENINE(37)-N6)-METHYLTRANSFERASEcoord: 12..279
IPR040372YaeB-likePANTHERPTHR12818UNCHARACTERIZEDcoord: 12..279
IPR036413YaeB-like superfamilySUPERFAMILY118196YaeB-likecoord: 12..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0015960.1Sed0015960.1mRNA
Sed0015960.2Sed0015960.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016430 tRNA (adenine-N6-)-methyltransferase activity