Sed0015523 (gene) Chayote v1

Overview


Name	Sed0015523
Type	gene
Organism	Sechium edule (Chayote v1)
Description	tRNA pseudouridine synthase family protein
Location	LG01: 18324727 .. 18327299 (+)
RNA-Seq Expression	Sed0015523
Synteny	Sed0015523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAAAACTGTCGGACACACTGTTAAAGCCCTAATTTTCCAGTCCACTTCCTTGCGTGAGTAAAATGGAAGAAACCCCGCCAACGGAAACCGGAAGATCACCGCCACCACCGCCGATCGAAGAACCAGATCCGAAGAAGCTCAAAATTTCATCCACAACCACCACCTCCGGCGAAGAAGTTACCAATTCCAATCCCGCGGCACCGAAGAAAGAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGCGGCGTCGGTTACCAAGGGATGCAGAAGAATCCAGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCCTCTATCTCGCCGGAGCAGTACCGGAGCACGCCCGAGGGACCTCGGAGGGCTACGATTGGTCCCGCTCTGCTCGCACCGATAAGGGCGTGAGCGCCGTCGGCCAGGTTGTTTCCGGCCGCTTCTACATCGATCCACCGGGTTTTGTCGATCGTCTCAATTCCAATCTCTCGCCTCAGATACGAGTTTTCGGCTATAAGCGCGTAACCGCGTCGTTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTACGTATACCTACTTCCTGTATTTTCTCTTGATCCTGATTGCCATCGTGATGGAGTAAGTCTAAAGGCTAGTTTAGGGTCTGATAACAAGCTTGTTGAGTGCTTAGAGTGCTCTGGACGAGGACGAAAGGTTGAAGGCTCGATTAAAACTGCGGTAACTCATTCAGACATTTCGTCGAACACGAGAGATGCTATTGAAGTTTCTGAAATTCAGGAAAATGGGGTCTTGGGGGACACGGCCACAACCTCTGATTCTAACCAAACTAGTGTTTTAGTCGAAAATAGTGAAACTGAGCTCAATGCTGGAAGTGAATGTACTGCAAAAAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGTGTTAATGGCAACGAGAATCCTTCGGGGAGCTTTAAATATGGCGAGGAGGTGAAGGCTAGGTTCAATAGGATTTTGAGGAACTATGAAGGAACTCATAACTTCCATAACTTCACTACCAGAATGAAACCAGAGGACCCTGCCGCTTATCGATTCATCGTTGCTTTCGATGCTGCTACCACCGTCGTGGTTGATGGCATTGAATTTGTGAAATGTGAAGTTGTTGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCAGTGGCAATCATGAGGAACTGTGCTCCTGAATCATTGATCAAAAAGGCTTTGCGAAAGTAAGTAATTGCCATTTTTTTTAAAACAAGGGTAACCACGCCCGTTCCTAGGCAAGACAAAGTAATTGTCATTTGAAACTACCATTTCATTTTATCTTATATTGATAGATATAACTACTATTTATTTGATCAATCAACAAATAAACTGAAAAAACATAACTATACTTAACAATAAAACACTTGAAGAAGATCACACACGTCGAAGCGAATTTGTAGGTTCAATTCCTACTGGATATTTTGGTAGAGTCTAGCTCTATCAAAAGATCCCTCTCTCAATAATGAGTCAATCCAATATTCAAGTTGTATAGATTGCGGAGGTTATCTAGTATCTTTATTATTTTTCTATTATAATATATATTTGGTTCTTATAACATTTTTAAAGAATTAAACTGAACCATGAACCATCATAGATTGGCCCAGCCGCCCGAGTGGTCAAGAGAGCCTCAGAAAACCAATAATTTGTGGGTTCAATTCATTTGTAGCCACCTACCTCATACTTCTACGGGTTTTCTTGATATCCAAGTGTTGTAGGGTTAGATTGGTTGCTCGTGAGAATAGTCAAGGTGTGCATAAGTTAACCAGATACTCACGGTCACGGATATCAAAAAAACAAAAATAAGTGGATTCCATGAACTCTTGGTGATCAAAATTCAAACACAGGGATGTAAATTTCAATGTTCCCACTGCACCTGAGGTTGGGCTGTACTTAGAGGAGTGTCTATTCACATCATATAATCAGAAGTGGAAAGACAGCCATGAAGAGGTATCAATGAAAGCATACGAAGAAGCAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCCTCATGGCTGCATTCTCTAAACCATCGCAATTATTCAGATTTAAATGCTGTCGATGAAGTTGCAGATAATGCTAAGAACACAAGTGTTGACATGGAGATACCAGCTGAAGCTTAAAAGGTCTTCTCTTACCTTTGCTTTAACTTGCCGTTTGGATGCATTGATCGAAAAATATTTATATTTTATGATTTAGTGCTTGTTTCATTTACACTCCAGTCAGATCAAGCAGAACTTTTGATTAGGAAAATGAGGAATATCAATCTATTAGTTAAGTTCCTATTACTGTCTCTAACTCAAGTTTTTTTTTGTACATTCAAAAGACCATGACCTCTTGGTTACGGGCGCACGTATATGTCGGTTGAGCTCTTGTTCTGGATGGTTTGACTCATGTTCTTATATACCCCCTCAAGGATTGAAATATTCATACTGG

mRNA sequence

AAAAAAAAAAAAAAAACTGTCGGACACACTGTTAAAGCCCTAATTTTCCAGTCCACTTCCTTGCGTGAGTAAAATGGAAGAAACCCCGCCAACGGAAACCGGAAGATCACCGCCACCACCGCCGATCGAAGAACCAGATCCGAAGAAGCTCAAAATTTCATCCACAACCACCACCTCCGGCGAAGAAGTTACCAATTCCAATCCCGCGGCACCGAAGAAAGAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGCGGCGTCGGTTACCAAGGGATGCAGAAGAATCCAGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCCTCTATCTCGCCGGAGCAGTACCGGAGCACGCCCGAGGGACCTCGGAGGGCTACGATTGGTCCCGCTCTGCTCGCACCGATAAGGGCGTGAGCGCCGTCGGCCAGGTTGTTTCCGGCCGCTTCTACATCGATCCACCGGGTTTTGTCGATCGTCTCAATTCCAATCTCTCGCCTCAGATACGAGTTTTCGGCTATAAGCGCGTAACCGCGTCGTTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTACGTATACCTACTTCCTGTATTTTCTCTTGATCCTGATTGCCATCGTGATGGAGTAAGTCTAAAGGCTAGTTTAGGGTCTGATAACAAGCTTGTTGAGTGCTTAGAGTGCTCTGGACGAGGACGAAAGGTTGAAGGCTCGATTAAAACTGCGGTAACTCATTCAGACATTTCGTCGAACACGAGAGATGCTATTGAAGTTTCTGAAATTCAGGAAAATGGGGTCTTGGGGGACACGGCCACAACCTCTGATTCTAACCAAACTAGTGTTTTAGTCGAAAATAGTGAAACTGAGCTCAATGCTGGAAGTGAATGTACTGCAAAAAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGTGTTAATGGCAACGAGAATCCTTCGGGGAGCTTTAAATATGGCGAGGAGGTGAAGGCTAGGTTCAATAGGATTTTGAGGAACTATGAAGGAACTCATAACTTCCATAACTTCACTACCAGAATGAAACCAGAGGACCCTGCCGCTTATCGATTCATCGTTGCTTTCGATGCTGCTACCACCGTCGTGGTTGATGGCATTGAATTTGTGAAATGTGAAGTTGTTGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCAGTGGCAATCATGAGGAACTGTGCTCCTGAATCATTGATCAAAAAGGCTTTGCGAAAGGATGTAAATTTCAATGTTCCCACTGCACCTGAGGTTGGGCTGTACTTAGAGGAGTGTCTATTCACATCATATAATCAGAAGTGGAAAGACAGCCATGAAGAGGTATCAATGAAAGCATACGAAGAAGCAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCCTCATGGCTGCATTCTCTAAACCATCGCAATTATTCAGATTTAAATGCTGTCGATGAAGTTGCAGATAATGCTAAGAACACAAGTGTTGACATGGAGATACCAGCTGAAGCTTAAAAGACCATGACCTCTTGGTTACGGGCGCACGTATATGTCGGTTGAGCTCTTGTTCTGGATGGTTTGACTCATGTTCTTATATACCCCCTCAAGGATTGAAATATTCATACTGG

Coding sequence (CDS)

ATGGAAGAAACCCCGCCAACGGAAACCGGAAGATCACCGCCACCACCGCCGATCGAAGAACCAGATCCGAAGAAGCTCAAAATTTCATCCACAACCACCACCTCCGGCGAAGAAGTTACCAATTCCAATCCCGCGGCACCGAAGAAAGAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGCGGCGTCGGTTACCAAGGGATGCAGAAGAATCCAGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCCTCTATCTCGCCGGAGCAGTACCGGAGCACGCCCGAGGGACCTCGGAGGGCTACGATTGGTCCCGCTCTGCTCGCACCGATAAGGGCGTGAGCGCCGTCGGCCAGGTTGTTTCCGGCCGCTTCTACATCGATCCACCGGGTTTTGTCGATCGTCTCAATTCCAATCTCTCGCCTCAGATACGAGTTTTCGGCTATAAGCGCGTAACCGCGTCGTTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTACGTATACCTACTTCCTGTATTTTCTCTTGATCCTGATTGCCATCGTGATGGAGTAAGTCTAAAGGCTAGTTTAGGGTCTGATAACAAGCTTGTTGAGTGCTTAGAGTGCTCTGGACGAGGACGAAAGGTTGAAGGCTCGATTAAAACTGCGGTAACTCATTCAGACATTTCGTCGAACACGAGAGATGCTATTGAAGTTTCTGAAATTCAGGAAAATGGGGTCTTGGGGGACACGGCCACAACCTCTGATTCTAACCAAACTAGTGTTTTAGTCGAAAATAGTGAAACTGAGCTCAATGCTGGAAGTGAATGTACTGCAAAAAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGTGTTAATGGCAACGAGAATCCTTCGGGGAGCTTTAAATATGGCGAGGAGGTGAAGGCTAGGTTCAATAGGATTTTGAGGAACTATGAAGGAACTCATAACTTCCATAACTTCACTACCAGAATGAAACCAGAGGACCCTGCCGCTTATCGATTCATCGTTGCTTTCGATGCTGCTACCACCGTCGTGGTTGATGGCATTGAATTTGTGAAATGTGAAGTTGTTGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCAGTGGCAATCATGAGGAACTGTGCTCCTGAATCATTGATCAAAAAGGCTTTGCGAAAGGATGTAAATTTCAATGTTCCCACTGCACCTGAGGTTGGGCTGTACTTAGAGGAGTGTCTATTCACATCATATAATCAGAAGTGGAAAGACAGCCATGAAGAGGTATCAATGAAAGCATACGAAGAAGCAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCCTCATGGCTGCATTCTCTAAACCATCGCAATTATTCAGATTTAAATGCTGTCGATGAAGTTGCAGATAATGCTAAGAACACAAGTGTTGACATGGAGATACCAGCTGAAGCTTAA

Protein sequence

MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLDPDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHSDISSNTRDAIEVSEIQENGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGAVASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAEA

Homology

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BLAST of Sed0015523 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo] >KAA0064620.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa] >TYK19972.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 833.2 bits (2151), Expect = 1.2e-237
Identity = 432/518 (83.40%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEPDPKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSN  +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TEL   SE  AK   SEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E PSGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+TTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKNTSVDME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of Sed0015523 vs. NCBI nr
Match: XP_004145528.1 (tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_012098 [Cucumis sativus])

HSP 1 Score: 829.3 bits (2141), Expect = 1.8e-236
Identity = 430/518 (83.01%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEP PKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSNT +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TELN GSE TAK   SEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E  SGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+  VVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEEAAEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKN S+DME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAE 518

[Close]

BLAST of Sed0015523 vs. NCBI nr
Match: XP_038899088.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 825.1 bits (2130), Expect = 3.3e-235
Identity = 432/518 (83.40%), Postives = 461/518 (89.00%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET  SPP P IEEPDPKKLK+SSTTTTS +E  NS+ AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRISPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSSSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+  S ISSNT +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAIIESHISSNTGNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++QEN VL D AT SDSN Q+SVLVENS TELN  SE TAK   SEMSGLGNDSEI
Sbjct: 241 IEVSDVQENRVLEDNATISDSNKQSSVLVENSCTELNVESESTAKSVGSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNGNE PSGSFKY EE KARFNRIL+NY GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGNEKPSGSFKYCEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+TTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKDSHEEVSMKAYEE AEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL AVDEVA+ A NTSV ME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRAVDEVAEIAMNTSVAMETQAE 518

[Close]

BLAST of Sed0015523 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 817.0 bits (2109), Expect = 9.1e-233
Identity = 422/518 (81.47%), Postives = 454/518 (87.64%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPT+T RSPP P IEEP+PKKLK+SSTTTTS +E  NSN AAPKK+RYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGS N+LV+CLECS RGRKVEG        +KTA++ SDIS N  +A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSEIQENGVLGDTATTSDS-NQTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NG L D AT SDS  +TSVLVENS TELN GSE  AK   SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSK+SVN NE PSGSFKYGEE KARFNRIL+ Y GTHNFHNFTTR K EDPAA RFI++
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDAA TVVV+G+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL++DVN N
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKDSHEEVSMKAYEE AEDFKMK IYSHI STEHKEGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL   DE ADNAKNTSVD+E  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAE 518

[Close]

BLAST of Sed0015523 vs. NCBI nr
Match: KAG6591243.1 (hypothetical protein SDJN03_13589, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024127.1 PUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 810.4 bits (2092), Expect = 8.5e-231
Identity = 421/519 (81.12%), Postives = 454/519 (87.48%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPT+T RSPP P IEEP+PKKLK+SSTTTTS +E  NSN AAPKK+RYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGS N+LV+CLECS RGRKVEG        +KTA++ SDIS N+ +A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNSGNA 240

Query: 241 IEVSEIQENGVLGDTATTSDS-NQTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NG L D AT SDS  +TSVLVENS TELN G E TAK   SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGGEATAKIEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           V SKESVN NE  SGSF+YG+E KARFNRIL+ Y GTHNFHNFTTR K EDPAA RFI++
Sbjct: 301 VASKESVNRNEKSSGSFEYGKEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDAATTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL++DVN N
Sbjct: 361 FDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKDSHEEVSMKAYEE AEDFKMK IYSHI STEHKEGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVA-DNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL   DE A DNAKNTSVD+E  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADDNAKNTSVDIETQAE 519

[Close]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 5.1e-53
Identity = 143/467 (30.62%), Postives = 223/467 (47.75%), Query Frame = 0


Query: 7   TETGRSPPPPPIEEPDPKKLKISSTTTTSGEE---VTNSNPAAPKKERYKRRKIAIFFAY 66
           T   +S P P   E D K+LK         E    V  +    PK  R  +RK+A+   Y
Sbjct: 98  TRVRKSSPSPSANENDKKRLKKEFQPRQKIEYTPLVDENGQPIPKAPRKPKRKVAVMLGY 157

Query: 67  CGVGYQGM--QKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAVG 126
           CG GY GM  Q +P  KTIE D+ +A+  AGA+        +   + R+ARTDKGV A G
Sbjct: 158 CGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTDKGVHAAG 217

Query: 127 QVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLDP 186
            V+S +  I+ P  ++++N  L  QIR++G +R T  F+ +K C  R Y YLLP FSL P
Sbjct: 218 NVISLKMIIEDPEIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFSLLP 277

Query: 187 DCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHSDISSNTRDAIEVSEIQE 246
              +   S+ + L  + KL                      + D+  + ++ I+  E  +
Sbjct: 278 PKPK---SVLSELVKEKKL---------------------ENPDLFEDDQEGIDWWENVK 337

Query: 247 NGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGNEN 306
           + +L    T    +  +   +  + +     +  A  E   +   S   + K+       
Sbjct: 338 SKILASGITQEQIDSITSSYDEQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQ 397

Query: 307 PSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVVDGI 366
              S++        F  +++ YEGTHNFHNFT     +D +A RF++    +   V++G 
Sbjct: 398 SRRSYRISSSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSANRFMIKTIVSDPFVIEGT 457

Query: 367 EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGLYLE 426
           E++  ++ GQSFMLHQIRKM+ +A  ++R   P  +I     +    N+P AP +GL LE
Sbjct: 458 EWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIINNFF-QSTKINIPKAPALGLLLE 517

Query: 427 ECLFTSYNQK-WKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKE 468
             +F  YN K  K  +E +    +++   +FKMK IY  I + E KE
Sbjct: 518 NPVFDGYNIKLTKSDYEPIDFTKFDKEMNEFKMKYIYDKIYAEESKE 539

[Close]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot
Match: Q9Y606 (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 202.2 bits (513), Expect = 1.4e-50
Identity = 140/441 (31.75%), Postives = 198/441 (44.90%), Query Frame = 0


Query: 48  KKERYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHARGTSEG 107
           ++E+  +RKI +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE+       
Sbjct: 77  RREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRK 136

Query: 108 YDWSRSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKF 167
             + R ARTDKGVSA GQVVS + ++     ++++NS+L   IR+ G KRVT  FN+K  
Sbjct: 137 MSFQRCARTDKGVSAAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVTGGFNSKNR 196

Query: 168 CDRRRYVYLLPVFSLDPDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHS 227
           CD R Y YLLP F+     H+D                                      
Sbjct: 197 CDARTYCYLLPTFAF---AHKD-------------------------------------R 256

Query: 228 DISSNTRDAIEVSEIQENGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLG 287
           D+   T                                                      
Sbjct: 257 DVQDET------------------------------------------------------ 316

Query: 288 NDSEIVVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAY 347
                                ++   E   + NR+L  Y+GTHNFHNFT++  P+DP+A 
Sbjct: 317 ---------------------YRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSAC 376

Query: 348 RFIVAFDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRK 407
           R+I+        V +G+EF    V GQSFM+HQIRKM+GL VAI++  APES+++++   
Sbjct: 377 RYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT 400

Query: 408 DVNFNVPTAPEVGLYLEECLFTSYNQKWKDS--HEEVSMKAYEEAAEDFKMKQIYSHIAS 467
           +   +VP AP +GL LE   F  YNQ++ +   HE +     E     FK + IY  I  
Sbjct: 437 E-KVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIG 400

Query: 468 TEHKEGAVASWLHSLNHRNYS 484
           TE  E ++A WL +L   N+S
Sbjct: 497 TERDERSMAQWLSTLPIHNFS 400

[Close]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 5.3e-50
Identity = 139/425 (32.71%), Postives = 208/425 (48.94%), Query Frame = 0


Query: 44  PAAPKKERYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSE 103
           P    + R  +RK+A    YCG GY GMQ NP  KTIE D+ +A   AGA+ ++     +
Sbjct: 74  PLVADEVRQPKRKVACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPK 133

Query: 104 GYDWSRSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKK 163
              + R+ARTDKGV A G V+S +  I+    V+++NS+L  Q+RV+G  R   +F  +K
Sbjct: 134 KSAFMRAARTDKGVHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRK 193

Query: 164 FCDRRRYVYLLPVFS-LDPDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVT 223
            C  R Y YL+P +S L+P   R G  +   L  D          G     EG       
Sbjct: 194 LCSSRVYEYLMPTYSFLNP---RPGTVMSEKLLKD----------GTSPDEEGKKYWESV 253

Query: 224 HSDISSNTRDAIEVSEIQENGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSG 283
            +D+ S     +   E  +   + +             ++  ET+  A SE    S+   
Sbjct: 254 AADLES---QGVSYDEWMKRACIDE-------------IKGEETKEVAESEVKTDSK--- 313

Query: 284 LGNDSEIVVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPA 343
              D+  +   ++V         F+   E  A+   IL+ YEGTHNFHNFT     +DP+
Sbjct: 314 --TDAATLEKIKAVERRHRE--EFRISGERLAKIREILKIYEGTHNFHNFTLGKAFKDPS 373

Query: 344 AYRFIVAFDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPESLIKKA 403
           A R + +   +   ++DG E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+ LI + 
Sbjct: 374 AMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIPQT 433

Query: 404 LRKDVNFNVPTAPEVGLYLEECLFTSYNQKWKD--SHEEVSMKAYEEAAEDFKMKQIYSH 463
             K    N+P AP +GL LE  ++ SYN+K +     E V    + +  E FK K IY  
Sbjct: 434 FEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIYDK 461

Query: 464 IASTE 465
           I + E
Sbjct: 494 IYAEE 461

[Close]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 199.5 bits (506), Expect = 9.0e-50
Identity = 151/486 (31.07%), Postives = 214/486 (44.03%), Query Frame = 0


Query: 5   PPTETGRSPPPPPIEEPDPKKLK--ISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIFFA 64
           PP    + PP  P  +PD K     +   T    + V       P + +  +RKI +  A
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPR-KLPKRKIVLLMA 88

Query: 65  YCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSA 124
           Y G GY GMQ+N G+   +TIE DL  AL  AG +PE+         + R ARTDKGVSA
Sbjct: 89  YSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSA 148

Query: 125 VGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSL 184
            GQVVS + ++     +D++NS+L   IR+ G KRVT  FN+K  CD R Y Y+LP F  
Sbjct: 149 AGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTF-- 208

Query: 185 DPDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHSDISSNTRDAIEVSEI 244
                                                   A  H D            ++
Sbjct: 209 ----------------------------------------AFAHKD-----------RDV 268

Query: 245 QENGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGN 304
           Q+                                                          
Sbjct: 269 QDE--------------------------------------------------------- 328

Query: 305 ENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVVD 364
                S++   E   + NR+L  Y+GTHNFHNFT++  P +P+A R+I+        V +
Sbjct: 329 -----SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVRE 388

Query: 365 GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGLY 424
           G+EF   +V GQSFM+HQIRKM+GL VAI++  APES+++++  ++   +VP AP +GL 
Sbjct: 389 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLV 396

Query: 425 LEECLFTSYNQKWKDS--HEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGAVASWLHSL 484
           LE   F  YNQ++     HE +     E     FK + IY  I STE  E ++A WL++L
Sbjct: 449 LERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTL 396

[Close]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 7.6e-49
Identity = 150/486 (30.86%), Postives = 213/486 (43.83%), Query Frame = 0


Query: 5   PPTETGRSPPPPPIEEPDPKKLK--ISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIFFA 64
           PP    + PP     +PD K     +   T    + V       P + +  +RKI +  A
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPR-KLPKRKIVLLMA 88

Query: 65  YCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSA 124
           Y G GY GMQ+N G+   +TIE DL  AL  AG +PE+         + R ARTDKGVSA
Sbjct: 89  YSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSA 148

Query: 125 VGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSL 184
            GQVVS + ++     +D++NS+L   IR+ G KRVT  FN+K  CD R Y Y+LP F  
Sbjct: 149 AGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTF-- 208

Query: 185 DPDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHSDISSNTRDAIEVSEI 244
                                                   A  H D            ++
Sbjct: 209 ----------------------------------------AFAHKD-----------RDV 268

Query: 245 QENGVLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGN 304
           Q+                                                          
Sbjct: 269 QDE--------------------------------------------------------- 328

Query: 305 ENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVVD 364
                S++   E   + NR+L  Y+GTHNFHNFT++  P +P+A R+I+        V +
Sbjct: 329 -----SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVRE 388

Query: 365 GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGLY 424
           G+EF   +V GQSFM+HQIRKM+GL VAI++  APES+++++  ++   +VP AP +GL 
Sbjct: 389 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLV 396

Query: 425 LEECLFTSYNQKWKDS--HEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGAVASWLHSL 484
           LE   F  YNQ++     HE +     E     FK + IY  I STE  E ++A WL++L
Sbjct: 449 LERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTL 396

[Close]

BLAST of Sed0015523 vs. ExPASy TrEMBL
Match: A0A1S3BV07 (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.9e-238
Identity = 432/518 (83.40%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEPDPKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSN  +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TEL   SE  AK   SEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E PSGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+TTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKNTSVDME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of Sed0015523 vs. ExPASy TrEMBL
Match: A0A5A7VBT0 (tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00860 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.9e-238
Identity = 432/518 (83.40%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEPDPKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSN  +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TEL   SE  AK   SEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E PSGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+TTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKNTSVDME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of Sed0015523 vs. ExPASy TrEMBL
Match: E5GCD2 (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.9e-238
Identity = 432/518 (83.40%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEPDPKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSN  +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TEL   SE  AK   SEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E PSGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+TTVVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKNTSVDME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of Sed0015523 vs. ExPASy TrEMBL
Match: A0A0A0L0J9 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.6e-237
Identity = 430/518 (83.01%), Postives = 463/518 (89.38%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPTET RSPP P IEEP PKKLK+SSTTTTS +E  NSN AAPKK+RYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGSDN+LV+CLECS RGRKVEG        +KTA+T SDISSNT +A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSEIQENGVLGDTATTSDSN-QTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NGVL D+ATTSDSN QTSVLVEN+ TELN GSE TAK   SEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSKESVNG E  SGSFKYGEE KARFNRIL++Y GTHNFHNFTTR K EDPAA RFIV+
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDA+  VVV+GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL+KDVN N
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKD+HEEVSMKAYEEAAEDFKMKQIYSHIASTEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL  +DEVA  AKN S+DME  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAE 518

[Close]

BLAST of Sed0015523 vs. ExPASy TrEMBL
Match: A0A6J1IJ76 (tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.4e-233
Identity = 422/518 (81.47%), Postives = 454/518 (87.64%), Query Frame = 0


Query: 1   MEETPPTETGRSPPPPPIEEPDPKKLKISSTTTTSGEEVTNSNPAAPKKERYKRRKIAIF 60
           MEE PPT+T RSPP P IEEP+PKKLK+SSTTTTS +E  NSN AAPKK+RYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE  RG S+ YDW+RSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIR+FGYKRVTASFNAKKFCDRRRYVYL+PVF+LD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PDCHRDGVSLKASLGSDNKLVECLECSGRGRKVEG-------SIKTAVTHSDISSNTRDA 240
           P+CHRD  S+KASLGS N+LV+CLECS RGRKVEG        +KTA++ SDIS N  +A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSEIQENGVLGDTATTSDS-NQTSVLVENSETELNAGSECTAK---SEMSGLGNDSEI 300
           IEVS++Q NG L D AT SDS  +TSVLVENS TELN GSE  AK   SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGNENPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVA 360
           VVSK+SVN NE PSGSFKYGEE KARFNRIL+ Y GTHNFHNFTTR K EDPAA RFI++
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFN 420
           FDAA TVVV+G+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI+KAL++DVN N
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGA 480
           VPTAPEVGLYL+ECLFTSYNQKWKDSHEEVSMKAYEE AEDFKMK IYSHI STEHKEGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VASWLHSLNHRNYSDLNAVDEVADNAKNTSVDMEIPAE 508
           VA WLHSLNHRNY DL   DE ADNAKNTSVD+E  AE
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAE 518

[Close]

BLAST of Sed0015523 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 274/489 (56.03%), Postives = 343/489 (70.14%), Query Frame = 0


Query: 17  PIEEPDPKKLKISST---TTTSGEEVTNSNPAAPKKERYKRRKIAIFFAYCGVGYQGMQK 76
           P  EPD KKLKIS+T   T +S      SN A  +  +Y+RRK+AI FA+CGVGYQGMQK
Sbjct: 17  PDREPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQK 76

Query: 77  NPGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAVGQVVSGRFYIDPP 136
           NPGAKTIE +LEEAL+ AGAVP+  R     Y+W+RSARTDKGVSAVGQVVSGRFY+DPP
Sbjct: 77  NPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYVDPP 136

Query: 137 GFVDRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLDPDCHRDGVSLKAS 196
           GFV+RLNS L  QIRVFGYKRV  SF++KKFCDRRRYVYL+PVF+LDP  H +   ++  
Sbjct: 137 GFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTD 196

Query: 197 LGSDNKLVECLECSGRGRKVEGSI----------KTAVTHSDISSNTRDAIEVSEIQENG 256
           LG +   V+C+ECS +G K+   +          K+    SDISS+T DAI      E  
Sbjct: 197 LGYE--YVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCE-- 256

Query: 257 VLGDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGNE--- 316
            L  +  ++++N  S +++ ++      S  ++K+E    G +    ++K  +N  E   
Sbjct: 257 TLSSSVPSAENNLNSEVLDGADV-----SALSSKAE----GMEESNTLAKVEMNNGEGED 316

Query: 317 --NPSGSFKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVV 376
                  F YGE+ K RFNRIL  Y G+HNFHNFTTR K  DPAA R+I++F+A T + +
Sbjct: 317 MTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVINL 376

Query: 377 DGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGL 436
           DG +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLI+ + +KDV  NVP APEVGL
Sbjct: 377 DGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGL 436

Query: 437 YLEECLFTSYNQKWKDSHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKEGAVASWLHSLN 488
           YL+EC FTSYN+++K SHEEVSM+ Y+E AE+FK K +YSHI S E K+GAVA WLHSLN
Sbjct: 437 YLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGSAEEKDGAVAIWLHSLN 492

[Close]

BLAST of Sed0015523 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 476.9 bits (1226), Expect = 2.1e-134
Identity = 260/456 (57.02%), Postives = 320/456 (70.18%), Query Frame = 0


Query: 32  TTTSGEEVTNSNPAAPKKERYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLA 91
           TTT+ EE+  S      +     +K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ A
Sbjct: 3   TTTTDEEIDLSCDGGVTEP----QKVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHA 62

Query: 92  GAVPEHARGTSEGYDWSRSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFG 151
           GAVPE  RG  + YD++RSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIR+FG
Sbjct: 63  GAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIFG 122

Query: 152 YKRVTASFNAKKFCDRRRYVYLLPVFSLDPDCHRDGVSLKASLGSDNKLVECLECSGRGR 211
           YK VT SF++KKFCDRRRYVYLLPVF+LDP  HRD  ++ ASLG   + V+C ECS RGR
Sbjct: 123 YKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGR 182

Query: 212 KVEGSIKTAVTHSDISSNTRDAIEVSEIQENGVLGDTATTSDSNQTSVLVENSETELNAG 271
           K+   +      ++  + + D    S+I  N     +A  SD       ++      N  
Sbjct: 183 KIPPGLVGKWKGTNFGTKSLDF--QSDISSN---NSSALRSD-------IKIEALSSNLA 242

Query: 272 SECTAKSEMSGLGNDSEIVVSKESVNGNENP-SGSFKYGEEVKARFNRILRNYEGTHNFH 331
             C+   E+  +  DS     K + N +E      F YGE+ K RF+RIL  Y G++NFH
Sbjct: 243 GLCSVDVEVGRIQEDS----CKLNTNSSETKVKSKFCYGEKEKERFSRILSCYVGSYNFH 302

Query: 332 NFTTRMKPEDPAAYRFIVAFDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMR 391
           NFTTR K +DP A R I++F A T + +DGI+F+KCEV+G+SFMLHQIRKMMGLAVAIMR
Sbjct: 303 NFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMR 362

Query: 392 NCAPESLIKKALRKDVNFNVPTAPEVGLYLEECLFTSYNQKWKDSHEEVSMKAYEEAAED 451
           NCA ESLI+ A  KDVN  VP APEVGLYL+EC FTSYN+ ++DSHEEVSM+AY+E AE 
Sbjct: 363 NCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDSHEEVSMEAYKEEAEA 422

Query: 452 FKMKQIYSHIASTEHKEGAVASWLHSLNHRNYSDLN 487
           FK+K IYSHI +TE K G +A WLHSLN+RNY DLN
Sbjct: 423 FKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDLN 438

[Close]

BLAST of Sed0015523 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.4e-98
Identity = 202/374 (54.01%), Postives = 252/374 (67.38%), Query Frame = 0


Query: 32  TTTSGEEVTNSNPAAPKKERYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLA 91
           TTT+ EE+  S      +     +K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ A
Sbjct: 3   TTTTDEEIDLSCDGGVTEP----QKVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHA 62

Query: 92  GAVPEHARGTSEGYDWSRSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRVFG 151
           GAVPE  RG  + YD++RSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIR+FG
Sbjct: 63  GAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIFG 122

Query: 152 YKRVTASFNAKKFCDRRRYVYLLPVFSLDPDCHRDGVSLKASLGSDNKLVECLECSGRGR 211
           YK VT SF++KKFCDRRRYVYLLPVF+LDP  HRD  ++ ASLG   + V+C ECS RGR
Sbjct: 123 YKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGR 182

Query: 212 KVEGSIKTAVTHSDISSNTRDAIEVSEIQENGVLGDTATTSDSNQTSVLVENSETELNAG 271
           K+   +      ++  + + D    S+I  N     +A  SD       ++      N  
Sbjct: 183 KIPPGLVGKWKGTNFGTKSLDF--QSDISSN---NSSALRSD-------IKIEALSSNLA 242

Query: 272 SECTAKSEMSGLGNDSEIVVSKESVNGNENP-SGSFKYGEEVKARFNRILRNYEGTHNFH 331
             C+   E+  +  DS     K + N +E      F YGE+ K RF+RIL  Y G++NFH
Sbjct: 243 GLCSVDVEVGRIQEDS----CKLNTNSSETKVKSKFCYGEKEKERFSRILSCYVGSYNFH 302

Query: 332 NFTTRMKPEDPAAYRFIVAFDAATTVVVDGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMR 391
           NFTTR K +DP A R I++F A T + +DGI+F+KCEV+G+SFMLHQIRKMMGLAVAIMR
Sbjct: 303 NFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMR 356

Query: 392 NCAPESLIKKALRK 405
           NCA ESLI+ A  K
Sbjct: 363 NCASESLIQSAFSK 356

[Close]

BLAST of Sed0015523 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-25
Identity = 116/415 (27.95%), Postives = 178/415 (42.89%), Query Frame = 0


Query: 23  PKKLKISST-----TTTSGEEVTNSNPAAPKKERYKRRKIAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS    ++ N  A K E Y+++K+ I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEHARGTSEGYDWSRSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + +   G      W+RS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNSNLSPQIRVFGYKRVTASFNAKKFCDRRRYVYLLPVFSLDPDCHR 202
              DP G V    ++ +L   IRVF        F+ ++ C  R+Y YLLPV         
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPV--------- 205

Query: 203 DGVSLKASLGSDNKLVECLECSGRGRKVEGSIKTAVTHSDISSNTRDAIEVSEIQENGVL 262
           D + +K S  SD       + +   ++ EG  +    +    S  R   E    Q NG  
Sbjct: 206 DVLGIKNSFTSDEIDYHITDFNEILKEFEG--EYPFHNYTQRSRYRRKSEQKIKQRNGRP 265

Query: 263 GDTATTSDSNQTSVLVENSETELNAGSECTAKSEMSGLGNDSEIVVSKESVNGNENPSGS 322
                +  ++++    EN    +  G E   + E    G   E VV+ +S N        
Sbjct: 266 PREPKSIKASESEFREEN---HIEIGEE---EEEKEVDGESDEHVVTPDSDNSQVYSRAK 325

Query: 323 FKYGEEVKARFNRILRNYEGTHNFHNFTTRMKPEDPAAYRFIVAFDAATTVVVDGIEFVK 382
           + Y  +   + +       G H    F  R    + +                 G  FV+
Sbjct: 326 WLYEPDETDKIS-------GAHFRKVFRCRSGKLENSL----------------GFGFVE 385

Query: 383 CEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIKKALRKDVNFNVPTAPEVGLYL 421
             + G+SFMLHQIRKM+G AVA+ R   P  +I+ +L K     +P AP   L L
Sbjct: 386 ISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLIL 400

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BLAST of Sed0015523 vs. TAIR 10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein )

HSP 1 Score: 58.2 bits (139), Expect = 2.3e-08
Identity = 31/54 (57.41%), Postives = 36/54 (66.67%), Query Frame = 0


Query: 435 SHEEVSMKAYEEAAED-FKMKQIYSHIASTEHKEGAVASWLHSLNHRNYSDLNA 488
           S  EVS+   +E   D FK K +YSHI S E K+GAVA WLHSLN RNY DL +
Sbjct: 82  SWREVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLRS 135

The following BLAST results are available for this feature:

BLAST of Sed0015523 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008452911.1	1.2e-237	83.40	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_004145528.1	1.8e-236	83.01	tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein...	[more]
XP_038899088.1	3.3e-235	83.40	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_022975443.1	9.1e-233	81.47	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
KAG6591243.1	8.5e-231	81.12	hypothetical protein SDJN03_13589, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Sed0015523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q59S63	5.1e-53	30.62	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q9Y606	1.4e-50	31.75	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]
Q6CC39	5.3e-50	32.71	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q4KM92	9.0e-50	31.07	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]
Q9WU56	7.6e-49	30.86	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]

BLAST of Sed0015523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BV07	5.9e-238	83.40	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7VBT0	5.9e-238	83.40	tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
E5GCD2	5.9e-238	83.40	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
A0A0A0L0J9	8.6e-237	83.01	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G65...	[more]
A0A6J1IJ76	4.4e-233	81.47	tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 S...	[more]

BLAST of Sed0015523 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20370.1	1.6e-142	56.03	Pseudouridine synthase family protein	[more]
AT1G76120.1	2.1e-134	57.02	Pseudouridine synthase family protein	[more]
AT1G76120.2	2.4e-98	54.01	Pseudouridine synthase family protein	[more]
AT2G30320.1	2.1e-25	27.95	Pseudouridine synthase family protein	[more]
AT1G20290.1	2.3e-08	57.41	SWI-SNF-related chromatin binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 46..165 e-value: 5.2E-29 score: 102.7
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 321..425 e-value: 2.9E-13 score: 50.3
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 287..489 e-value: 5.1E-54 score: 184.9
IPR001406	Pseudouridine synthase I, TruA	TIGRFAM	TIGR00071	TIGR00071	coord: 55..419 e-value: 4.9E-43 score: 145.4
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 32..488
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..42
None	No IPR available	PANTHER	PTHR11142:SF24	TRNA PSEUDOURIDINE SYNTHASE	coord: 32..488
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 59..425 e-value: 7.74103E-101 score: 301.455
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0015523.1	Sed0015523.1	mRNA
Sed0015523.2	Sed0015523.2	mRNA
Sed0015523.3	Sed0015523.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding