Homology
BLAST of Sed0015318 vs. NCBI nr
Match:
XP_022953917.1 (putative disease resistance protein RGA4 [Cucurbita moschata])
HSP 1 Score: 902.9 bits (2332), Expect = 2.6e-258
Identity = 532/1000 (53.20%), Postives = 670/1000 (67.00%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEGVL N A +++ +LSS A QQLGM+ GV+D+L LKS+VSAI+AV+ DAEERQ KS
Sbjct: 1 MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
LKDWL+KL EALYDA+DVLDEIS +A+R RG+ AKQVRIFFSR N F+YKM+R
Sbjct: 61 FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120
Query: 126 EIKIIRERLDEISAEKDKFGFIETSSTPRMIQHDGLSH-QMG--THSAFIEEEVVGREDD 185
+IK +RERL I+AE+ F E + QH MG THS EEV+GR+D
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIG--QKTQHGSFDQIHMGAETHSFSNNEEVIGRDDH 180
Query: 186 KMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQ 245
K ++K LL D++ N+SFIAIVGM GIGKTTLAR L++D +VS+ FDLK+WVWVS
Sbjct: 181 KRVIKDLLQDMN--VKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQN 240
Query: 246 FDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKP 305
F+VK VVE++IE A+ S+P V G+E+L +LQ+++ GKKYLLVMDD+W+ESE+KW E K
Sbjct: 241 FEVKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKN 300
Query: 306 LLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK---KIGSPK 365
LLM GARGSKI+ITKRDS + E+ +MT LI+L+GLS+ SWSLF+KVAF + +PK
Sbjct: 301 LLMGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPK 360
Query: 366 MIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALS 425
+IQLGKEIL KCGG+PLVIRHIGRLL+ + SEEEWISFKN++L + + ++D+ +IL LS
Sbjct: 361 LIQLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLS 420
Query: 426 YKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELC 485
Y HLP +LKQCFAYSSL KY + KDELIRQW+A GFVQS G KSME NDYF ELC
Sbjct: 421 YNHLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELC 480
Query: 486 WRFFYE------HHDGYVCMHDKMHDLAGMVTHNENI--------------YHFSLGHNM 545
WRFFYE + + V +HD MHDLA MV NE + H +
Sbjct: 481 WRFFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREI 540
Query: 546 KAWKDFPSELLEAKGLRT--LRKSLL---NFMDKVDLAKLLSNFPRLRVLDLSNANIYMV 605
W+ PS+L +AKGLRT L +L N M++V L +L F RLRVLDL +NI V
Sbjct: 541 GIWEHVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600
Query: 606 PKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRH 665
P ++K+L HLRYLDLS N ++SLPN + LVNLQTL L C L +LPR +L NLRH
Sbjct: 601 PDSIKRLTHLRYLDLSGNY-MKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRH 660
Query: 666 LELDGRSKLTHMPKEIGKLSCLQTLSLFVLD--EKKKGSKLSELKELIDLRGGLYIKGLE 725
L + + LTHMP+ +GKL+CLQTLS FVLD + GSKL EL L DLRG L I GLE
Sbjct: 661 LNISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLE 720
Query: 726 HLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCI 785
+L+ P EA LANLK+KKGLR L L WK S DE + DE IL GLE +P +ES I
Sbjct: 721 NLKFTPSEASLANLKEKKGLRHLTLIWKNLS-GDEHRFQA-DEEILAGLEPNPAIESFRI 780
Query: 786 WRYKGQELPNW--VSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIID 845
Y G +LPNW +S+P+ KLT I + C RLQ LPQ LPALK L+L LTSL+F ID
Sbjct: 781 SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKF-ID 840
Query: 846 NNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGV 905
N +P SS+ +FFPSLE L L +MPNLEAWWE ES V +
Sbjct: 841 NTDPYSSA----LFFPSLESLCLMDMPNLEAWWERESMVEE------------------- 900
Query: 906 LGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKKVELNKVGVWMVTSI----- 963
+ LP F LT LSI +CPKL +P P L + L+ VG+ M++SI
Sbjct: 901 ----------EFLPTFPCLTILSISNCPKLATMPGHPPLTDITLDGVGLQMMSSIRSSSN 954
BLAST of Sed0015318 vs. NCBI nr
Match:
XP_023547878.1 (putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 900.2 bits (2325), Expect = 1.7e-257
Identity = 529/1000 (52.90%), Postives = 671/1000 (67.10%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEGVL N A +++ +LSS A QQLGM+ GV+D+L L+S+VSAI+AV+ DAEERQ KS
Sbjct: 4 MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELRSSVSAIKAVVLDAEERQTKSH 63
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
LKDWL+KL EALYDA+DVLDEIS +A+R RG+ AKQVRIFFSR N F+YK++R
Sbjct: 64 FLKDWLEKLKEALYDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKITR 123
Query: 126 EIKIIRERLDEISAEKDKFGFIETSSTPRMIQHDGLSH-QMG--THSAFIEEEVVGREDD 185
+IK +RERL+ I+AE+ F E + QH MG THS EEV+GR+D
Sbjct: 124 QIKNVRERLEVIAAERKNFHLTEIIG--QKTQHGSFDQIHMGAETHSFSNNEEVIGRDDH 183
Query: 186 KMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQ 245
K ++K LL D++ N+SFIAIVGM GIGKTTLAR L++D +VS+ FDLK+WVWVS
Sbjct: 184 KRVIKDLLQDMN--VKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQN 243
Query: 246 FDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKP 305
F+VK VVE++IE A+ S+P V G+E+L +LQ+++ GKKYLLVMDD+W+ESE+KW E K
Sbjct: 244 FEVKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKN 303
Query: 306 LLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK---KIGSPK 365
LLM GARGSKI+ITKRDS + E+ +MT LI+L+GLS+ SWSLF+KVAF + +PK
Sbjct: 304 LLMGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPK 363
Query: 366 MIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALS 425
+IQLGKEIL KCGG+PLVIRHIGRLL+ + SEEEWISFKN++L + + ++D+ +IL LS
Sbjct: 364 LIQLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLS 423
Query: 426 YKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELC 485
Y HLP +LKQCFAYSSL KY + KDELIRQW+A GFVQS G KSME NDYF ELC
Sbjct: 424 YNHLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELC 483
Query: 486 WRFFYE------HHDGYVCMHDKMHDLAGMVTHNENI--------------YHFSLGHNM 545
WRFFYE + + V +HD MHDLA MV NE + H +
Sbjct: 484 WRFFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREI 543
Query: 546 KAWKDFPSELLEAKGLRT--LRKSLL---NFMDKVDLAKLLSNFPRLRVLDLSNANIYMV 605
W+ PS+L +AKGLRT L +L N M++V L +L F RLRVLDL +NI V
Sbjct: 544 GIWEHVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 603
Query: 606 PKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRH 665
P ++K+L HLRYLDLS N ++SLPN + LVNLQTL L C L +LPR +L NLRH
Sbjct: 604 PDSIKRLTHLRYLDLSGNY-MKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRH 663
Query: 666 LELDGRSKLTHMPKEIGKLSCLQTLSLFVLD--EKKKGSKLSELKELIDLRGGLYIKGLE 725
L + + LTHMP+ +GKL+CLQTLS FVLD + GSKL EL L DLRG L I GLE
Sbjct: 664 LNISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLE 723
Query: 726 HLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCI 785
+L+ P EA LANLK+KKGLR L L WK S DE + DE IL GLE +P +ES I
Sbjct: 724 NLKFTPSEASLANLKEKKGLRHLTLIWKNLS-GDEHRFQA-DEEILAGLEPNPAIESFRI 783
Query: 786 WRYKGQELPNW--VSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIID 845
Y G +LPNW +S+P+ KLT I + C RLQ LPQ LPALK L+L LTSL+F ID
Sbjct: 784 SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKF-ID 843
Query: 846 NNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGV 905
N +P SS+ +FFPSLE L L +MPNLEAWWE ES V +
Sbjct: 844 NTDPYSSA----LFFPSLESLCLMDMPNLEAWWERESMVEE------------------- 903
Query: 906 LGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKKVELNKVGVWMVTSI----- 963
+ LP F LT LSI +CPKL +P P L + L+ VG+ M++SI
Sbjct: 904 ----------EFLPTFPCLTILSISNCPKLATMPGHPPLTDITLDGVGLQMMSSIRSSSN 957
BLAST of Sed0015318 vs. NCBI nr
Match:
XP_038896095.1 (putative disease resistance protein RGA4 [Benincasa hispida])
HSP 1 Score: 883.6 bits (2282), Expect = 1.6e-252
Identity = 507/1004 (50.50%), Postives = 667/1004 (66.43%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEG+L N AG ++ +LSS A QQLGM+ GV DDL +L S+VSAI+AVL DAEERQ S
Sbjct: 1 MAEGILFNLAGEIIKKLSSLAFQQLGMLWGVNDDLTKLTSSVSAIKAVLLDAEERQTGSN 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
L+DWL KL EALYDA+DVLDEIS +AL R+VMT GK AKQVRIFFS+SN FNYKM+R
Sbjct: 61 SLQDWLGKLKEALYDAEDVLDEISYEALHREVMTAGKKAKQVRIFFSKSNPVAFNYKMAR 120
Query: 126 EIKIIRERLDEISAEKDKFGFIETSSTP-RMIQHDGLSHQMGTHSAFIEEEVVGREDDKM 185
+IK +RERL I+AE+ +F F E + D + T S EEV+GR+ K
Sbjct: 121 QIKNVRERLGVIAAERKEFHFAEMFGQKFQYPLFDQIHIGAETRSKSNNEEVIGRDGHKR 180
Query: 186 LVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFD 245
++K LL D+ HN+SFIAIVGMGGIGKTTLAR L++D +VSK+FDLK+WVWVS F+
Sbjct: 181 VIKDLLQDMK--VKHNISFIAIVGMGGIGKTTLARSLFNDAQVSKYFDLKIWVWVSQNFE 240
Query: 246 VKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLL 305
VK V+E++IE + S+P V+G+E+L +LQ++I GKKYLLVMDD+W+ESE+KW + K LL
Sbjct: 241 VKIVMEKIIESITESRPNVQGMESLQNQLQKMIQGKKYLLVMDDIWNESEQKWFDLKNLL 300
Query: 306 MCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK---KIGSPKMI 365
M GARGSKI+ITKRDS + E+ +MT LI+L+GLS+ +SWSL ++VAF + +PK I
Sbjct: 301 MGGARGSKIMITKRDSTLAVEISNMTNLITLKGLSECNSWSLLKRVAFMDGIEPTNPKFI 360
Query: 366 QLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSYK 425
++GKEI+ KCGG+PL+IRHIGRLL++ SEEEW SFKN++LSR+ + + ++ IL LSY
Sbjct: 361 KVGKEIVVKCGGVPLIIRHIGRLLYNNTSEEEWTSFKNNQLSRVIQHDQNMIPILRLSYN 420
Query: 426 HLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELCWR 485
+LPP+LK+CFAYSSL K+ + KDELI+QW+A GF+Q+ KSME I NDYF ELCWR
Sbjct: 421 YLPPNLKRCFAYSSLLLKHWPIHKDELIQQWMAQGFIQASNCGKSMEDIGNDYFMELCWR 480
Query: 486 FFYEH----HDGYVCMHDKMHDLAGMVTHNENI---------------YHFSLGHNMKAW 545
FF+EH D Y+ +HD MHDLA V NE + H S H +K+W
Sbjct: 481 FFFEHSGRVSDSYIFVHDVMHDLAKTVAGNEYVCISSKNNYDNVSEKTRHVSFDHKIKSW 540
Query: 546 KDFPSELLEAKGLRTLRKSLLNF-----MDKVDLAKLLSNFPRLRVLDLSNANIYMVPKA 605
D L + +GLRT S + ++KV L L S+FPRLRVL L ++ I +VPK+
Sbjct: 541 WDDLPTLCKGEGLRTFLPSYYPYKAVVEVNKVSLNALFSSFPRLRVLCLGDSEIKIVPKS 600
Query: 606 LKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRHLEL 665
++KL+ LRYLDLS N + SLPN I +L +L+TL L C L ELPR +L NLRHL L
Sbjct: 601 IEKLRQLRYLDLSGNENMVSLPNSISKLQHLETLNLKRCFALRELPRGTCNLVNLRHLYL 660
Query: 666 DGRSKLTHMPKEIGKLSCLQTLSLFVLD---EKKKGSKLSELKELIDLRGGLYIKGLEHL 725
LT+MP+ +GKL+CLQTLS FVLD E + SKLSEL L DLRG L I GL+ L
Sbjct: 661 P--LCLTYMPEGMGKLTCLQTLSFFVLDNNREDTRSSKLSELNGLKDLRGCLTIDGLDQL 720
Query: 726 RGNPYEAKLANLKDKKGLRDLVLQWK----VRSKADESKDGLNDESILEGLELHPNVESV 785
R NP EA ANLKDKKGL++L L W V+ KADE+ G +D ++EGLE NVE +
Sbjct: 721 RSNPKEASSANLKDKKGLQNLQLTWNLSKDVKYKADEA-GGCSDVKVMEGLEPQQNVEYL 780
Query: 786 CIWRYKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIID 845
I Y G LP+W+S P+ KLT I + +C+RLQ L Q + LP+L+++ L + ++F ID
Sbjct: 781 SIHGYSGARLPDWMSRPLLKLTSIRIQECHRLQHLTQLNYLPSLREVILRSIIPVKF-ID 840
Query: 846 NNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGV 905
N+P SS VFFPSL+ L L+NMPNL+ WWE ES
Sbjct: 841 KNDPCPSS----VFFPSLKTLCLENMPNLKGWWEKESTA--------------------- 900
Query: 906 LGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKKVELNKVGVWMVTSIG---- 963
L P F LT LSI +CPKL +P P L+ ++L+ VG+ M++SIG
Sbjct: 901 ----------GLRPAFPCLTTLSISNCPKLASIPEHPCLENLKLDDVGLQMMSSIGTSLD 960
BLAST of Sed0015318 vs. NCBI nr
Match:
XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 879.0 bits (2270), Expect = 3.9e-251
Identity = 509/1009 (50.45%), Postives = 668/1009 (66.20%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAE +L AG +L +LSSQA Q+LGM+ G++ DL +L + VS I+ VL DAE RQ KS
Sbjct: 1 MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRG-KHAKQVRIFFSRSNQPTFNYKMS 125
LL++WL KL EALYDA+DVLDE+ST+ALRR++MTR K+AKQVRIFFS+SNQ FNY+M+
Sbjct: 61 LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120
Query: 126 REIKIIRERLDEISAEKDKFGFIET-SSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDK 185
R+IK I ERLD I AEK +F E S + D + T S+ +EEV+GR+DD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180
Query: 186 MLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQF 245
VK L D++ HNVSFIAI GMGGIGKTTLA+ LY+D EVS FDLK+WVWVSDQF
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240
Query: 246 DVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPL 305
+V+ V E+MIE A+ + P VKG+EAL KLQ++IG +KYLLVMDD+W+ESE+KW K L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300
Query: 306 LMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAF---KKIGSPKM 365
LM GARGSK+LITKRD V E++SMT L +LEGLS+ +SW LF KVAF K+ P
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360
Query: 366 IQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSY 425
I LGKEIL +CGG+PLVIRH+GR+L+SK S+EEW+SFK++EL + +++ND+T+IL LSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420
Query: 426 KHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELCW 485
HLPP+LK+CFAYSSL+ K ++ +LIRQW+A GF++ G KS+E DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480
Query: 486 RFFYEHH------DGYVCMHDKMHDLAGMVTHN---------------ENIYHFSLGHNM 545
RFFY + + VCMHD M + V N E H S + +
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540
Query: 546 KAWKDFPSELLEAKGLRTL-----RKSLLNFMDKVDLAKLLSNFPRLRVLDLSNANIYMV 605
++W+D S+L +AKGLRT+ +N +DK L +L S+FPRLRVLDL + I +V
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600
Query: 606 PKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRH 665
PK++KKL+HLRYLDLS N ++ +P+ I EL NLQTL L C L ELPRDI +L NLRH
Sbjct: 601 PKSIKKLRHLRYLDLSEN-DMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRH 660
Query: 666 LELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIKGLEHL 725
L + ++T + + KL+CLQT+SLFV D KK +KL EL +L L G L I GLE L
Sbjct: 661 LTFEPCMEVTPTSEGMEKLTCLQTISLFVFD-CKKTNKLWELNDLSYLTGELKIIGLEKL 720
Query: 726 RGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCIWR 785
R +P E L NLKDKKG + L L+WK+ + +G DE+I+EGLE HPNVES+ I
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKL---GKDEYEGEADETIMEGLEPHPNVESLSING 780
Query: 786 YKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIIDNNNP 845
Y G LPNWV N + KLT I + C R+Q LPQF+QL L+ LHL L SL+F ID ++P
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEF-IDKSDP 840
Query: 846 GSSSSSTFVFFPSLEILILKNMPNLEAWWE-GESRVRDGKSKKTGLLPDLKEWMKGVLGD 905
SSS VFFPSL+ L L++MPNLE WWE GES+ V+
Sbjct: 841 YSSS----VFFPSLKFLRLEDMPNLEGWWELGESK---------------------VVAR 900
Query: 906 EESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSL-KKVELNKVGVWMVTSIGPSSNLE 965
E S K + L P F ++ L I+ CPKL +P+ S+ V L+ +GV MV++IGP S+
Sbjct: 901 ETSGKAKWLPPTFPQVNFLRIYGCPKLSSMPKLASIGADVILHDIGVQMVSTIGPVSSFM 960
Query: 966 YVTLKNIEDLKHI----PMDLRRSS--------SIKKVKVSNCPPFTSV 969
++++ + +LK++ DL SS S++ + +S CP S+
Sbjct: 961 FLSMHGMTNLKYLWEEFQQDLVSSSTSTMSSPISLRYLTISGCPYLMSL 978
BLAST of Sed0015318 vs. NCBI nr
Match:
KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])
HSP 1 Score: 879.0 bits (2270), Expect = 3.9e-251
Identity = 509/1009 (50.45%), Postives = 668/1009 (66.20%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAE +L AG +L +LSSQA Q+LGM+ G++ DL +L + VS I+ VL DAE RQ KS
Sbjct: 1 MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRG-KHAKQVRIFFSRSNQPTFNYKMS 125
LL++WL KL EALYDA+DVLDE+ST+ALRR++MTR K+AKQVRIFFS+SNQ FNY+M+
Sbjct: 61 LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120
Query: 126 REIKIIRERLDEISAEKDKFGFIET-SSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDK 185
R+IK I ERLD I AEK +F E S + D + T S+ +EEV+GR+DD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180
Query: 186 MLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQF 245
VK L D++ HNVSFIAI GMGGIGKTTLA+ LY+D EVS FDLK+WVWVSDQF
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240
Query: 246 DVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPL 305
+V+ V E+MIE A+ + P VKG+EAL KLQ++IG +KYLLVMDD+W+ESE+KW K L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300
Query: 306 LMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAF---KKIGSPKM 365
LM GARGSK+LITKRD V E++SMT L +LEGLS+ +SW LF KVAF K+ P
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360
Query: 366 IQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSY 425
I LGKEIL +CGG+PLVIRH+GR+L+SK S+EEW+SFK++EL + +++ND+T+IL LSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420
Query: 426 KHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELCW 485
HLPP+LK+CFAYSSL+ K ++ +LIRQW+A GF++ G KS+E DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480
Query: 486 RFFYEHH------DGYVCMHDKMHDLAGMVTHN---------------ENIYHFSLGHNM 545
RFFY + + VCMHD M + V N E H S + +
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540
Query: 546 KAWKDFPSELLEAKGLRTL-----RKSLLNFMDKVDLAKLLSNFPRLRVLDLSNANIYMV 605
++W+D S+L +AKGLRT+ +N +DK L +L S+FPRLRVLDL + I +V
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600
Query: 606 PKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRH 665
PK++KKL+HLRYLDLS N ++ +P+ I EL NLQTL L C L ELPRDI +L NLRH
Sbjct: 601 PKSIKKLRHLRYLDLSEN-DMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRH 660
Query: 666 LELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIKGLEHL 725
L + ++T + + KL+CLQT+SLFV D KK +KL EL +L L G L I GLE L
Sbjct: 661 LTFEPCMEVTPTSEGMEKLTCLQTISLFVFD-CKKTNKLWELNDLSYLTGELKIIGLEKL 720
Query: 726 RGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCIWR 785
R +P E L NLKDKKG + L L+WK+ + +G DE+I+EGLE HPNVES+ I
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKL---GKDEYEGEADETIMEGLEPHPNVESLSING 780
Query: 786 YKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIIDNNNP 845
Y G LPNWV N + KLT I + C R+Q LPQF+QL L+ LHL L SL+F ID ++P
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEF-IDKSDP 840
Query: 846 GSSSSSTFVFFPSLEILILKNMPNLEAWWE-GESRVRDGKSKKTGLLPDLKEWMKGVLGD 905
SSS VFFPSL+ L L++MPNLE WWE GES+ V+
Sbjct: 841 YSSS----VFFPSLKFLRLEDMPNLEGWWELGESK---------------------VVAR 900
Query: 906 EESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSL-KKVELNKVGVWMVTSIGPSSNLE 965
E S K + L P F ++ L I+ CPKL +P+ S+ V L+ +GV MV++IGP S+
Sbjct: 901 ETSGKAKWLPPTFPQVNFLRIYGCPKLSSMPKLASIGADVILHDIGVQMVSTIGPVSSFM 960
Query: 966 YVTLKNIEDLKHI----PMDLRRSS--------SIKKVKVSNCPPFTSV 969
++++ + +LK++ DL SS S++ + +S CP S+
Sbjct: 961 FLSMHGMTNLKYLWEEFQQDLVSSSTSTMSSPISLRYLTISGCPYLMSL 978
BLAST of Sed0015318 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 459.5 bits (1181), Expect = 9.8e-128
Identity = 326/973 (33.50%), Postives = 514/973 (52.83%), Query Frame = 0
Query: 29 QLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEALYDADDVLDEI 88
+LG+V G E + K+L S S IQAVL DA+E+Q K K +K+WL+KL A Y+ DD+LD+
Sbjct: 20 ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79
Query: 89 STQALR-RQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIRERLDEISAEKDKFGFI 148
T+A R +Q + H + + TF YK+ + +K + E+LD I+ E+ F
Sbjct: 80 KTEAARFKQAVLGRYHPRTI----------TFCYKVGKRMKEMMEKLDAIAEERRNFHLD 139
Query: 149 ETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDKMLVKRLLFDIDDVA-GHNVSFIAI 208
E R+I+ Q G E +V GRE ++ + ++L I++V+ V + I
Sbjct: 140 E-----RIIERQAARRQTG--FVLTEPKVYGREKEEDEIVKIL--INNVSYSEEVPVLPI 199
Query: 209 VGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELASGSQPKVKGI 268
+GMGG+GKTTLA+++++D +++HF+LK+WV VSD FD KR+++ ++E G +
Sbjct: 200 LGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDL 259
Query: 269 EALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILITKRDSIVLAEV 328
L KLQEL+ GK+Y LV+DD+W+E ++KW+ + +L GA G+ ILIT R + +
Sbjct: 260 APLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLE-KIGSI 319
Query: 329 RSMTKLISLEGLSDESSWSLFQKVAF--KKIGSPKMIQLGKEILGKCGGIPLVIRHIGRL 388
+L L LS E W LF++ AF + SPK++++GKEI+ KCGG+PL + +G L
Sbjct: 320 MGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGL 379
Query: 389 LFSKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFAYSSLYSKYEFVD 448
L K E EW ++ E+ + ++ N + L LSY HLP L+QCFAY +++ K ++
Sbjct: 380 LRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIE 439
Query: 449 KDELIRQWIAHGFVQSREGSKSMEIANDYFTELCWRFFYEHHD-----GYVCMHDKMHDL 508
K+ LI W+AH F+ S+ + ++ N+ + EL R F++ + Y MHD +HDL
Sbjct: 440 KEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDL 499
Query: 509 AGMVTHNENIYHFSLGHNMKAWKDFPSELLEAKGLRTLRKSLLNFMDKVD--LAKLLSNF 568
A + N+K +D + K + + + F + V L F
Sbjct: 500 ATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMS-----IGFSEVVSSYSPSLFKRF 559
Query: 569 PRLRVLDLSNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKT 628
LRVL+LSN+ +P ++ L HLRYLDLS N + SLP + +L NLQTL L C++
Sbjct: 560 VSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGN-KICSLPKRLCKLQNLQTLDLYNCQS 619
Query: 629 LTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKE 688
L+ LP+ L +LR+L LD LT MP IG L+CL+TL FV+ E +KG +L EL+
Sbjct: 620 LSCLPKQTSKLCSLRNLVLD-HCPLTSMPPRIGLLTCLKTLGYFVVGE-RKGYQLGELRN 679
Query: 689 LIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESIL 748
L +LRG + I LE ++ N EAK ANL K L L + W R ES+ + +L
Sbjct: 680 L-NLRGAISITHLERVK-NDMEAKEANLSAKANLHSLSMSWD-RPNRYESE----EVKVL 739
Query: 749 EGLELHPNVESVCIWRYKGQELPNWVSNPISK-LTHISLGKCNRLQRLPQFHQLPALKKL 808
E L+ HPN++ + I + G LP+W+++ + K + I + C LP F +LP L+ L
Sbjct: 740 EALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESL 799
Query: 809 HLEE-LTSLQFIIDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKK 868
L++ ++++ D S T FPSL L + NL+
Sbjct: 800 ELQDGSVEVEYVED------SGFLTRRRFPSLRKLHIGGFCNLK---------------- 859
Query: 869 TGLLPDLKEWMKGVLGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKKVEL-- 928
GL + MKG F L ++ I DCP + P S+KK+E+
Sbjct: 860 -GL-----QRMKGA-------------EQFPVLEEMKISDCPMFVF-PTLSSVKKLEIWG 915
Query: 929 --NKVGVWMVTSIGPSS----------------------NLEYVTLKNIEDLKHIPMDLR 963
+ G+ ++++ + NL Y+++ +E+LK +P L
Sbjct: 920 EADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKELPTSLA 915
BLAST of Sed0015318 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 445.3 bits (1144), Expect = 1.9e-123
Identity = 312/972 (32.10%), Postives = 511/972 (52.57%), Query Frame = 0
Query: 18 VLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEA 77
+L L+S +L ++ G E + ++L S S IQAVL DA+E+Q K K +++WL+KL A
Sbjct: 9 LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68
Query: 78 LYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIRERLDEI 137
Y+ DD+L E +A+R +Q R+ F F +K+ R +K I E+LD I
Sbjct: 69 AYEVDDILGECKNEAIR---------FEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAI 128
Query: 138 SAEKDKFGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDKMLVKRLLFDIDDVA 197
S E+ KF F+E ++ + + T E +V GR+ ++ + ++L + +VA
Sbjct: 129 SEERRKFHFLE-----KITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVA 188
Query: 198 GHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELAS 257
+ I+GMGG+GKTTLA+++++D V+KHF+ K+WV VSD FD KR+++ +I
Sbjct: 189 -EELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIE 248
Query: 258 GSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILITK 317
S P V+ + + KLQEL+ GK+YLLV+DD+W++ +KW + + +L GARG+ IL T
Sbjct: 249 RSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATT 308
Query: 318 RDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAF--KKIGSPKMIQLGKEILGKCGGIP 377
R V + + L LS S LF + AF +K +P ++ +GKEI+ KCGG+P
Sbjct: 309 RLEKV-GSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVP 368
Query: 378 LVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFAYSS 437
L + +G LL K E EW +++E+ + ++ + + L LSY HLP L+QCFAY +
Sbjct: 369 LAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCA 428
Query: 438 LYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFTELCWRFFYEHHDG-----YV 497
++ K + K+ LI W+AHGF+ S+ + ++ N+ + EL R F++ + Y
Sbjct: 429 VFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYF 488
Query: 498 CMHDKMHDLAGMVTHNENIYHFSLGHNMKAWKDFPSELLEAKGLRTLRKSLLNFMDKVD- 557
+HD +HDLA + N+K +K S + F V
Sbjct: 489 KIHDLIHDLATSLFSASASCGNIREINVKDYKHTVS---------------IGFAAVVSS 548
Query: 558 -LAKLLSNFPRLRVLDLSNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQ 617
LL F LRVL+LS + + +P ++ L HLRYLDLS N +SLP + +L NLQ
Sbjct: 549 YSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN-NFRSLPERLCKLQNLQ 608
Query: 618 TLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKK 677
TL ++ C +L LP+ L++LRHL +DG LT P IG L+CL+TL F++ KK
Sbjct: 609 TLDVHNCYSLNCLPKQTSKLSSLRHLVVDG-CPLTSTPPRIGLLTCLKTLGFFIVG-SKK 668
Query: 678 GSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESK 737
G +L ELK L +L G + I LE ++ N +A+ ANL K L+ L + W
Sbjct: 669 GYQLGELKNL-NLCGSISITHLERVK-NDTDAE-ANLSAKANLQSLSMSW--------DN 728
Query: 738 DGLN-----DESILEGLELHPNVESVCIWRYKGQELPNWVSNPI-SKLTHISLGKCNRLQ 797
DG N + +LE L+ HPN++ + I + G P+W+++ + K+ + + C
Sbjct: 729 DGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCL 788
Query: 798 RLPQFHQLPALKKLHLEELTSLQFIIDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWW 857
LP F +LP L+ L L+ ++ ++ ++ S ST FPSL+ L + +L+
Sbjct: 789 CLPPFGELPCLENLELQNGSAEVEYVEEDDV-HSRFSTRRSFPSLKKLRIWFFRSLKGLM 848
Query: 858 EGESRVRDGKSKKTGLL-------PDLKEWMKGVLGDEESEKRRDLLPNFARLTDLSIHD 917
+ E + ++ +L P L K + + + + N + LT L I
Sbjct: 849 KEEGEEKFPMLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGA 908
Query: 918 CPKLRWVPRQPSLKKVELNKVGVWMVTSIGPSSNLEYVTLKNIEDLKHIPMDLRRSSSIK 968
+ +P + M TS+ +NLE+++ + ++LK +P L +++K
Sbjct: 909 NYRATSLPEE--------------MFTSL---TNLEFLSFFDFKNLKDLPTSLTSLNALK 917
BLAST of Sed0015318 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 438.3 bits (1126), Expect = 2.3e-121
Identity = 308/973 (31.65%), Postives = 504/973 (51.80%), Query Frame = 0
Query: 18 VLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEA 77
VL L+S +L ++ G +D+ +RL S S IQAVL DA+E+Q K L++WL+KL A
Sbjct: 9 VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68
Query: 78 LYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIRERLDEI 137
Y+ DD+LDE T+A R G++ +V F +K+ + + + ++L+ I
Sbjct: 69 TYEVDDILDEYKTKATRFLQSEYGRYHPKV---------IPFRHKVGKRMDQVMKKLNAI 128
Query: 138 SAEKDKFGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDKMLVKRLLFDIDDVA 197
+ E+ KF E ++I+ + + G S E +V GR+ +K + ++L + A
Sbjct: 129 AEERKKFHLQE-----KIIERQAATRETG--SVLTEPQVYGRDKEKDEIVKILINTASDA 188
Query: 198 GHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELAS 257
+S + I+GMGG+GKTTL++++++D V++ F K+W+ +SD F+ KR+++ ++E
Sbjct: 189 -QKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIE 248
Query: 258 GSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILITK 317
G + L KLQEL+ GK+Y LV+DD+W+E + KW + +L GA G+ +L T
Sbjct: 249 GKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTT 308
Query: 318 RDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAF--KKIGSPKMIQLGKEILGKCGGIP 377
R V + + L LS E W LF + AF ++ +P ++ +GKEI+ KCGG+P
Sbjct: 309 RLEKV-GSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVP 368
Query: 378 LVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFAYSS 437
L + +G +L K E EW ++ + + ++ + + L LSY HLP L+QCF Y +
Sbjct: 369 LAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVYCA 428
Query: 438 LYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFTELCWRFFY-----EHHDGYV 497
++ K + K+ LI W+AHGF+ S+ + ++ N+ + EL R F+ E Y
Sbjct: 429 VFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYF 488
Query: 498 CMHDKMHDLAGMV----THNENIYHFSLGHNMKAWKDFPSELLEAKGLRTLRKSLLNFMD 557
MHD +HDLA + T + NI + ++ +E++ +
Sbjct: 489 KMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYS------------- 548
Query: 558 KVDLAKLLSNFPRLRVLDLSNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVN 617
LL F LRVL+L N+N+ +P ++ L HLRYLDLS N +++LP + +L N
Sbjct: 549 ----PSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQN 608
Query: 618 LQTLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEK 677
LQTL L+ C +L+ LP+ L +LR+L LDG S LT P IG L+CL++LS FV+ K
Sbjct: 609 LQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCS-LTSTPPRIGLLTCLKSLSCFVIG-K 668
Query: 678 KKGSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADE 737
+KG +L ELK L +L G + I L+ ++ + +AK ANL K L L L W + K
Sbjct: 669 RKGHQLGELKNL-NLYGSISITKLDRVKKDT-DAKEANLSAKANLHSLCLSWDLDGK--- 728
Query: 738 SKDGLNDESILEGLELHPNVESVCIWRYKGQELPNWVSNPISK-LTHISLGKCNRLQRLP 797
D +LE L+ H N++ + I + G LP+W++ + K + I + C LP
Sbjct: 729 ---HRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLP 788
Query: 798 QFHQLPALKKLHLEE-LTSLQFIIDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAW--W 857
F +LP L+ L L ++++ DN +PG FPSL L++ + NL+
Sbjct: 789 PFGELPCLESLELHTGSADVEYVEDNVHPGR--------FPSLRKLVIWDFSNLKGLLKM 848
Query: 858 EGESRVRDGKSK-----KTGLLPDLK--EWMKGVLGDEESEKRRDLLPNFARLTDLSIHD 917
EGE + + ++P L + +K ++ D + + N LT L I D
Sbjct: 849 EGEKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRS---ISNLRALTSLDISD 908
Query: 918 CPKLRWVPRQPSLKKVELNKVGVWMVTSIGPSSNLEYVTLKNIEDLKHIPMDLRRSSSIK 969
VE + M S+ +NL+Y+ + +LK +P L +++K
Sbjct: 909 --------------NVEATSLPEEMFKSL---ANLKYLKISFFRNLKELPTSLASLNALK 908
BLAST of Sed0015318 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 431.0 bits (1107), Expect = 3.7e-119
Identity = 304/968 (31.40%), Postives = 500/968 (51.65%), Query Frame = 0
Query: 18 VLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEA 77
+L L+S +L ++ G +D+ +RL S S IQAVL DA+E+Q +K L++WL+KL A
Sbjct: 9 LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68
Query: 78 LYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIRERLDEI 137
Y+ DD+LDE T+A R G++ +V F +K+ + + + ++L I
Sbjct: 69 TYEVDDILDEYKTKATRFSQSEYGRYHPKV---------IPFRHKVGKRMDQVMKKLKAI 128
Query: 138 SAEKDKFGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDK-MLVKRLLFDIDDV 197
+ E+ F E ++++ + + G S E +V GR+ +K +VK L+ ++ D
Sbjct: 129 AEERKNFHLHE-----KIVERQAVRRETG--SVLTEPQVYGRDKEKDEIVKILINNVSD- 188
Query: 198 AGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELA 257
++S + I+GMGG+GKTTLA+++++D V++HF K+W+ VS+ FD KR+++ ++E
Sbjct: 189 -AQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESI 248
Query: 258 SGSQPKV--KGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLLMCGARGSKIL 317
G +P + + L KLQEL+ GK+YLLV+DD+W+E ++KW + +L GA G+ +L
Sbjct: 249 EG-RPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVL 308
Query: 318 ITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAF--KKIGSPKMIQLGKEILGKCG 377
T R V + + L LS E W LF + AF ++ +P ++ +GKEI+ K G
Sbjct: 309 TTTRLEKV-GSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSG 368
Query: 378 GIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFA 437
G+PL + +G +L K E W ++ + + ++ + + L LSY LP LKQCFA
Sbjct: 369 GVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFA 428
Query: 438 YSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFTELCWRFFY---EHHDG- 497
Y +++ K ++K++LI W+AHGF+ S+ + ++ ++ + EL R F+ E DG
Sbjct: 429 YCAVFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGK 488
Query: 498 -YVCMHDKMHDLAGMVTHNENIYHFSLGHNMKAWKDFPSELLEAKGLRTLRKSLLNFMDK 557
Y MHD +HDLA + S + +R + K M
Sbjct: 489 TYFKMHDLIHDLATSLF---------------------SANTSSSNIREINKHSYTHMMS 548
Query: 558 VDLAKL--------LSNFPRLRVLDLSNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPN 617
+ A++ L F LRVL+L ++ +P ++ L HLRYL+L + G++SLP
Sbjct: 549 IGFAEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPK 608
Query: 618 FIGELVNLQTLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLS 677
+ +L NLQTL L C L LP++ L +LR+L LDG LT MP IG L+CL+TL
Sbjct: 609 QLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLG 668
Query: 678 LFVLDEKKKGSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWK 737
FV+ +KKG +L EL L +L G + I LE ++ N +AK ANL K L L + W
Sbjct: 669 QFVVG-RKKGYQLGELGNL-NLYGSIKISHLERVK-NDKDAKEANLSAKGNLHSLSMSWN 728
Query: 738 VRSKADESKDGLNDESILEGLELHPNVESVCIWRYKGQELPNWVSNPISK-LTHISLGKC 797
+ + +LE L+ H N+ S+ I+ ++G LP W+++ + K + I +
Sbjct: 729 NFGPHIYESE---EVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNF 788
Query: 798 NRLQRLPQFHQLPALKKLHLE-ELTSLQFIIDNNNPGSSSSSTFVFFPSLEILILKNMPN 857
LP F LP L+ L L ++++ + + S T + FPSL
Sbjct: 789 RNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLR--------K 848
Query: 858 LEAWWEGESRVRDGKSKKTGLLPDLKEWMKGVLGDEESEKRRDLLPNFARLTDLSIHDCP 917
L+ W G +KG+L E E+ F L ++ IH+CP
Sbjct: 849 LDIWDFGS--------------------LKGLLKKEGEEQ-------FPVLEEMIIHECP 893
Query: 918 KLRWVPRQPSLKKVEL--NKVGVWMVTSIGPS-SNLEYVTLKNIEDLKHIPMDLRRSSSI 963
L +L + + NKV + + +NL+Y+T+ +LK +P L +++
Sbjct: 909 FLTLSSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNAL 893
BLAST of Sed0015318 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 409.5 bits (1051), Expect = 1.2e-112
Identity = 306/980 (31.22%), Postives = 497/980 (50.71%), Query Frame = 0
Query: 37 EDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEALYDADDVLDEISTQALRRQ 96
E+ L+RL + + I AVL DAEE+Q + +++ W+ +L + +Y A+D LD+I+T+ALR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 97 VMTRGKHAKQVRIFFSRSNQPTF----NYKMSREIKIIRERLDEISAEKDKFGFIE-TSS 156
+ + ++R R + F + + ++ + RL+ ++++++ G E T+
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 157 TPRMIQHDGLSHQMGTHSAFIEEEVVGREDDKMLVKRLLFDIDDVAGHNVSFIAIVGMGG 216
P+ ++ T S E EV GR+DDK + R L ++ + ++ +AIVG+GG
Sbjct: 156 IPK--------QRLPTTSLVDESEVFGRDDDKDEIMRFLIP-ENGKDNGITVVAIVGIGG 215
Query: 217 IGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELASGSQPKVKGIEALCV 276
+GKTTL++LLY+D V +F K+W VS++FDV ++ +++ E + + ++ L V
Sbjct: 216 VGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQV 275
Query: 277 KLQELIGGK--KYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILITKRDSIVLAEVRSM 336
KL+E + G +LLV+DD+W+E+ W+ + + A+GS+IL+T R V A +
Sbjct: 276 KLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRV-ASIMCA 335
Query: 337 TKLISLEGLSDESSWSLFQKVAF---KKIGSPKMIQLGKEILGKCGGIPLVIRHIGRLLF 396
+ +L+ LSD WSLF K F + + ++ L + I+ KC G+PL ++ +G +L
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 397 SKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFAYSSLYSKYEFVDKD 456
+ EW + + + + ++L +L +SY +LP HLK+CFAY S++ K +KD
Sbjct: 396 FEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKD 455
Query: 457 ELIRQWIAHGFVQSREGSKSM-EIANDYFTELCWRFFYEHHDGYVCMHDKMHDLAGMVT- 516
+++ W+A GF+Q SK++ E+ N+YF+EL R + MHD +++LA +
Sbjct: 456 KVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQFASG 515
Query: 517 --------------HNENIYHFSLGHNMKAWKDFPSELLEAKGLRT-LRKSLLN-----F 576
Y L N +F + L E K LRT L SL N
Sbjct: 516 EFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA-LREVKFLRTFLPLSLTNSSRSCC 575
Query: 577 MDKVDLAKLLSNFPRLRVLDLSNANI-YMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGE 636
+D++ KLL RLRVL LS+ I + P K + H R+LDLS L+ LP +
Sbjct: 576 LDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCY 635
Query: 637 LVNLQTLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLSLFVL 696
+ NLQTL L+ C +L ELP DI +L NLR+L+L G +KL MP+ G+L LQTL+ F +
Sbjct: 636 MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 695
Query: 697 DEKKKGSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSK 756
GS++SEL L DL G L I L+ + + +A ANL KK LR++ W+ S
Sbjct: 696 -SASDGSRISELGGLHDLHGKLKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSS 755
Query: 757 ADESKDG----LNDESILEGLELHPNVESVCIWRYKGQELPNWVSNP-ISKLTHISLGKC 816
+ E+ N+ + E L H ++E + I RYKG+ P+W+S+P S++ I L +C
Sbjct: 756 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 815
Query: 817 NRLQRLPQFHQLPALKKLHLEELTSLQ-----FIIDNNNPGSSSSSTFVFFPSLEILILK 876
LP QLP LK+LH+ + LQ F + F SLE L
Sbjct: 816 QYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQP---FRSLETLRFD 875
Query: 877 NMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGVLGDEESEKRRDLLPNF-ARLTDLS 936
N+P+ + W D + + L P LK+ L + LP F L L
Sbjct: 876 NLPDWQEW-------LDVRVTRGDLFPSLKK-----LFILRCPELTGTLPTFLPSLISLH 935
Query: 937 IHDCPKLRWVPRQPSLKKVELNKVGVWMVTSIGPSSNLEYVTLKNIEDLKHIPMDLRRSS 973
I+ C L + P NL+ +++K+ D + L +
Sbjct: 936 IYKCGLLDFQPDHHEYS-----------------YRNLQTLSIKSSCDTL-VKFPLNHFA 967
BLAST of Sed0015318 vs. ExPASy TrEMBL
Match:
A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)
HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 532/1000 (53.20%), Postives = 670/1000 (67.00%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEGVL N A +++ +LSS A QQLGM+ GV+D+L LKS+VSAI+AV+ DAEERQ KS
Sbjct: 1 MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
LKDWL+KL EALYDA+DVLDEIS +A+R RG+ AKQVRIFFSR N F+YKM+R
Sbjct: 61 FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120
Query: 126 EIKIIRERLDEISAEKDKFGFIETSSTPRMIQHDGLSH-QMG--THSAFIEEEVVGREDD 185
+IK +RERL I+AE+ F E + QH MG THS EEV+GR+D
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIG--QKTQHGSFDQIHMGAETHSFSNNEEVIGRDDH 180
Query: 186 KMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQ 245
K ++K LL D++ N+SFIAIVGM GIGKTTLAR L++D +VS+ FDLK+WVWVS
Sbjct: 181 KRVIKDLLQDMN--VKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQN 240
Query: 246 FDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKP 305
F+VK VVE++IE A+ S+P V G+E+L +LQ+++ GKKYLLVMDD+W+ESE+KW E K
Sbjct: 241 FEVKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKN 300
Query: 306 LLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK---KIGSPK 365
LLM GARGSKI+ITKRDS + E+ +MT LI+L+GLS+ SWSLF+KVAF + +PK
Sbjct: 301 LLMGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPK 360
Query: 366 MIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALS 425
+IQLGKEIL KCGG+PLVIRHIGRLL+ + SEEEWISFKN++L + + ++D+ +IL LS
Sbjct: 361 LIQLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLS 420
Query: 426 YKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELC 485
Y HLP +LKQCFAYSSL KY + KDELIRQW+A GFVQS G KSME NDYF ELC
Sbjct: 421 YNHLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELC 480
Query: 486 WRFFYE------HHDGYVCMHDKMHDLAGMVTHNENI--------------YHFSLGHNM 545
WRFFYE + + V +HD MHDLA MV NE + H +
Sbjct: 481 WRFFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREI 540
Query: 546 KAWKDFPSELLEAKGLRT--LRKSLL---NFMDKVDLAKLLSNFPRLRVLDLSNANIYMV 605
W+ PS+L +AKGLRT L +L N M++V L +L F RLRVLDL +NI V
Sbjct: 541 GIWEHVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600
Query: 606 PKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRH 665
P ++K+L HLRYLDLS N ++SLPN + LVNLQTL L C L +LPR +L NLRH
Sbjct: 601 PDSIKRLTHLRYLDLSGNY-MKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRH 660
Query: 666 LELDGRSKLTHMPKEIGKLSCLQTLSLFVLD--EKKKGSKLSELKELIDLRGGLYIKGLE 725
L + + LTHMP+ +GKL+CLQTLS FVLD + GSKL EL L DLRG L I GLE
Sbjct: 661 LNISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLE 720
Query: 726 HLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCI 785
+L+ P EA LANLK+KKGLR L L WK S DE + DE IL GLE +P +ES I
Sbjct: 721 NLKFTPSEASLANLKEKKGLRHLTLIWKNLS-GDEHRFQA-DEEILAGLEPNPAIESFRI 780
Query: 786 WRYKGQELPNW--VSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIID 845
Y G +LPNW +S+P+ KLT I + C RLQ LPQ LPALK L+L LTSL+F ID
Sbjct: 781 SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKF-ID 840
Query: 846 NNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGV 905
N +P SS+ +FFPSLE L L +MPNLEAWWE ES V +
Sbjct: 841 NTDPYSSA----LFFPSLESLCLMDMPNLEAWWERESMVEE------------------- 900
Query: 906 LGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKKVELNKVGVWMVTSI----- 963
+ LP F LT LSI +CPKL +P P L + L+ VG+ M++SI
Sbjct: 901 ----------EFLPTFPCLTILSISNCPKLATMPGHPPLTDITLDGVGLQMMSSIRSSSN 954
BLAST of Sed0015318 vs. ExPASy TrEMBL
Match:
A0A6J1JVS7 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111488319 PE=4 SV=1)
HSP 1 Score: 875.2 bits (2260), Expect = 2.8e-250
Identity = 525/987 (53.19%), Postives = 660/987 (66.87%), Query Frame = 0
Query: 19 LAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEAL 78
L +LSS A QQLGM+ GV+D+L LKS+VSAI+AV+ DAEE Q KS LKDWL+KL EAL
Sbjct: 233 LKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEE-QTKSHFLKDWLEKLKEAL 292
Query: 79 YDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIRERLDEIS 138
YDA+DVLDEIS +A+R RG+ AKQVRIFFSR N F+YKM+ +IK +RERL+ I+
Sbjct: 293 YDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTCQIKNVRERLEVIA 352
Query: 139 AEKDKFGFIETSSTPRMIQHDGLSH-QMG--THSAFIEEEVVGREDDKMLVKRLLFDIDD 198
AE+ F E + QH MG THS EEV+GR+D K ++K LL D++
Sbjct: 353 AERKNFHLTEIIG--QKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKRVIKDLLQDMN- 412
Query: 199 VAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIEL 258
N+SFIAIVGM GIGKTTLAR L++D +VS+ FDLK+WVWVS F+VK VVE++IE
Sbjct: 413 -VKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVKIVVEKIIES 472
Query: 259 ASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILI 318
A+ S+P V G+E+L +LQ+++ GKKYLLVMDD+W+ESE+KW E K LLM GARGSKI+I
Sbjct: 473 ATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMGGARGSKIMI 532
Query: 319 TKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK---KIGSPKMIQLGKEILGKCG 378
TKRDS + E+ +MT LI+L+GLS+ SWSLF+KVAF + +PK+IQLGKEIL KCG
Sbjct: 533 TKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQLGKEILVKCG 592
Query: 379 GIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFA 438
G+PLVIRHIGRLL+ + SEEEWISFKN++L + + ++D+ +IL LSY HL +LKQCFA
Sbjct: 593 GVPLVIRHIGRLLYIQKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHLSSNLKQCFA 652
Query: 439 YSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTELCWRFFYE------H 498
YSSL KY + KDELIRQW+A GFVQS G KSME ANDYF ELCWRFFYE +
Sbjct: 653 YSSLLLKYRLIHKDELIRQWVAQGFVQSLSGCKSMEDTANDYFMELCWRFFYENFNDEHN 712
Query: 499 HDGYVCMHDKMHDLAGMVTHNENI--------------YHFSLGHNMKAWKDFPSELLEA 558
+ V +HD MHDLA MV NE + H + W+ PS+LL+A
Sbjct: 713 FEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVAESTRHILFDREIGIWEQVPSKLLKA 772
Query: 559 KGLRT--LRKSLL---NFMDKVDLAKLLSNFPRLRVLDLSNANIYMVPKALKKLKHLRYL 618
KGLRT L +L N M++V L +L F RLRVLDL +NI VP ++K+L HLRYL
Sbjct: 773 KGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSIKRLTHLRYL 832
Query: 619 DLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMP 678
DLS N ++SLPN + LVNLQTL L C L +LPR +L NLRHL + + LTHMP
Sbjct: 833 DLSGNY-MKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNISDSTHLTHMP 892
Query: 679 KEIGKLSCLQTLSLFVLD--EKKKGSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLAN 738
+ +GKL+CLQTLS FVLD + GSKL EL L DLRG L I GLE+L+ P EA LAN
Sbjct: 893 EGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKFTPSEASLAN 952
Query: 739 LKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVESVCIWRYKGQELPNW-- 798
LK+KKGLR L L WK S DE + DE IL GLE +P +ES I Y G +LPNW
Sbjct: 953 LKEKKGLRHLKLIWKNLS-GDEHRFQA-DEEILAGLEPNPAIESFRISGYFGVKLPNWLS 1012
Query: 799 VSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFIIDNNNPGSSSSSTFV 858
+S+P+ KLT I + C RLQ LPQ LPALK L+L LTSL+F IDN +P SS+ +
Sbjct: 1013 LSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKF-IDNTDPYSSA----L 1072
Query: 859 FFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGVLGDEESEKRRDLL 918
FFPSLE L L +M NLEAWWE ES V +EES L
Sbjct: 1073 FFPSLESLCLMDMLNLEAWWERESMV-----------------------EEES------L 1132
Query: 919 PNFARLTDLSIHDCPKLRWVPRQPSLKKVELNKVGVWMVTS------IGPSSNLEYVTLK 963
P F LT LSI +CPKL +P P L + L+ VG+ M++S PSS+L+ + LK
Sbjct: 1133 PTFPCLTILSISNCPKLATMPGHPPLTDITLDGVGLQMMSSRRSSSNTSPSSSLKSLELK 1172
BLAST of Sed0015318 vs. ExPASy TrEMBL
Match:
A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)
HSP 1 Score: 817.0 bits (2109), Expect = 8.9e-233
Identity = 488/985 (49.54%), Postives = 631/985 (64.06%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEER-QNKS 65
MAEGVL + AG +L +L S A Q+LGMV GVE+DL +L S VS I+ VL DAEE Q K+
Sbjct: 1 MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60
Query: 66 -KLLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKM 125
L+DWL+KL EALYDA+DVLDE+S++ L R+V+T+GK+AKQVRIFFS SNQ FNY++
Sbjct: 61 DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120
Query: 126 SREIKIIRERLDEISAEKDKFGFIETSSTPRMIQHDGLSHQMG---THSAFIEEEVVGRE 185
+R IK I ERLD I AE+ +F E + R + MG T S EEV+GR+
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTE-NCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRD 180
Query: 186 DDKMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVS 245
DK +K LL DI+ HNVSFI I GMGGIGKTTLA+ LYHD EVS+ FD +W+W+S
Sbjct: 181 YDKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWIS 240
Query: 246 DQFDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEF 305
DQF+V+ VVE++IE A+ +P V+G+EAL +LQE+IGGKKYLLVMDD+W E+E+KW E
Sbjct: 241 DQFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHEL 300
Query: 306 KPLLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFKKIGSP-- 365
KPLLM GA GSKILITKRDS V E+ SMT L +L+GL + +SWSLF+KVAFK+ P
Sbjct: 301 KPLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPAN 360
Query: 366 -KMIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRI---TRENNDLTN 425
+I+LGKEIL KCGG+PLVIRHIGRLL+SK SEEEW+SFKN+EL ++ T +N+ LT
Sbjct: 361 SNLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTP 420
Query: 426 ILALSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSM--EIAND 485
IL LSY HLPP+LKQCFAY SL+ K ++ +LIRQW+AHGF++S G+ + E+
Sbjct: 421 ILNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKS 480
Query: 486 YFTELCWRFFYE------HHDGYVCMHDKMHDLAGMVTHNENIY--------------HF 545
YF ELCWRFF+E + D V MHD M ++A V + ++ H
Sbjct: 481 YFKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHI 540
Query: 546 SLGHNMKAWKDFPSELLEAKGLRT-LRKSLLNFMDKVD------LAKLLSNFPRLRVLDL 605
+K W+D +L +AKGLRT L S F ++++ L +L S+FPRLRVL L
Sbjct: 541 LFDSKIKLWQDVLLKLQKAKGLRTFLFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGL 600
Query: 606 SNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDI 665
+++ I++VP ++K L+HLRYL+LS N ++SLP I +L NLQ+L L C L ELPRD
Sbjct: 601 NHSKIHVVPNSIKNLRHLRYLNLSRNY-MESLPTSITKLQNLQSLDLKDCYKLNELPRDT 660
Query: 666 IDLANLRHLEL-DGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGG 725
+L NLRHL L D HM + + KLSCL+TL FVLD K+ + SEL G
Sbjct: 661 SNLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLDCKRSNMQ-SELNVPNYFNGR 720
Query: 726 LYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHP 785
L I GLE LR P + L NLKD +GLRDL L WK+ + D+ +G DE ++EGL+
Sbjct: 721 LKIVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDERVMEGLQPQS 780
Query: 786 NVESVCIWRYKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSL 845
NVES+ I Y G LPNWVS +S LT I++ C+R+Q LPQ L LK + LE++ SL
Sbjct: 781 NVESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSL 840
Query: 846 QFIIDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKE 905
+F +D N PG + FPSL L LKNM NLE WWE
Sbjct: 841 KF-VDKNEPG------LLCFPSLVSLRLKNMGNLEGWWESR------------------- 900
Query: 906 WMKGVLGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPR--QPSLKKVELNKVGVWMVT 948
V+G + LP F L L I CPKL +P+ +L L VGV +VT
Sbjct: 901 ----VVG--------ETLPIFPELQYLRIQHCPKLSLMPKIASSTLDSFFLCDVGVQVVT 944
BLAST of Sed0015318 vs. ExPASy TrEMBL
Match:
A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)
HSP 1 Score: 795.0 bits (2052), Expect = 3.6e-226
Identity = 471/990 (47.58%), Postives = 627/990 (63.33%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEG+L AG +L +L S ++ GM+ G++DDL +LKS VS IQ+ L DA+
Sbjct: 255 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
+ WL+KL L+DA+D++DE + R+VMTR KHAK+VRIFFS SNQ FNYKM+R
Sbjct: 315 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374
Query: 126 EIKIIRERLDEISAEKD---KFGFIE--TSSTPRMIQHDGLSHQMGTHSAFIEEEVVGRE 185
+K IRERLD I EK F++ P I+ +S THS+ +E V+GR+
Sbjct: 375 RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRM-RMSLSRETHSSLNKERVIGRD 434
Query: 186 DDKMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVS 245
DDK +K LL D NVS IAIVGMGGIGKTTLA+ LY+D +VSKHF++KMW+WVS
Sbjct: 435 DDKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVS 494
Query: 246 DQFDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEF 305
QF K VV++++E A+ P + ++ + +LQ++IGGKKYLLVMD++ +ESE +W++
Sbjct: 495 KQFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKL 554
Query: 306 KPLLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK----KIG 365
K LL+ G RGSKI+ITKRD V +E+ MT ++L+GLS++ SWSLF++VAF+
Sbjct: 555 KDLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNPI 614
Query: 366 SPKMIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNIL 425
+PK+ +GKEI +CGG+PL IRH+GR L SK EEEW+S +D+L +T++NND+ +IL
Sbjct: 615 NPKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASIL 674
Query: 426 ALSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFT 485
LSY LP HLKQCFAYSSL+ K + +ELIRQW+A GF++S +G KSME I +YF
Sbjct: 675 ELSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 734
Query: 486 ELCWRFFYE------HHDGYVCMHDKMHDLA-----------GMVTHN----ENIYHFSL 545
ELCWRFFYE + D YV MHD M++LA G + +N E HFS
Sbjct: 735 ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFSF 794
Query: 546 GHNMKAWKDFPSELLEAKGLRTLR-----KSLLNFMDKVDLAKLLSNFPRLRVLDLSNAN 605
+ +++W+D S+L +AKGLRT N +++ +L +L S+FPRLRVL L +N
Sbjct: 795 DYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSN 854
Query: 606 IYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLA 665
I+++P ++KKLK LRYLDLS N ++SLPN I EL +LQTL L GC L E+PRDI L
Sbjct: 855 IHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLV 914
Query: 666 NLRHLELDGRSKLTHM-PKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIK 725
NLRHL+ S++THM P+ + KLSCLQT+SLFV D K +KL EL EL +L G L I
Sbjct: 915 NLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFD-CTKTNKLRELNELNNLTGELKIT 974
Query: 726 GLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVES 785
GL+ LR PYE L NLKDKK LR L L+WK+ E + DE +++GLE +PNVES
Sbjct: 975 GLQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGLYKHEDE---ADERVMKGLEPNPNVES 1034
Query: 786 VCIWRYKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFII 845
+ I Y G LP W+ N KLT I + C+RLQ LPQFH L LK L L +L SL+FI
Sbjct: 1035 LGIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFID 1094
Query: 846 DNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKG 905
+NP SSS +FFPSL+ L L NM NLE WWE +
Sbjct: 1095 KFDNPFSSS----MFFPSLKSLCLANMANLEGWWE----------------------LSR 1154
Query: 906 VLGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKK--VELNKVGVWMVTS-IG 955
+ E SE + L P F L L I+ CPKL +P+ PS K V L VGV +V S IG
Sbjct: 1155 AVAGETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIG 1212
BLAST of Sed0015318 vs. ExPASy TrEMBL
Match:
A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)
HSP 1 Score: 783.9 bits (2023), Expect = 8.4e-223
Identity = 470/991 (47.43%), Postives = 625/991 (63.07%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSK 65
MAEG+L AG +L +L S ++ GM+ G++DDL +LKS VS IQ+ L DA+
Sbjct: 1 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60
Query: 66 LLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSR 125
+ WL+KL L+DA+D++DE + R+VMTR KHAK+VRIFFS SNQ FNYKM+R
Sbjct: 61 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120
Query: 126 EIKIIRERLDEISAEKD---KFGFIE--TSSTPRMIQHDGLSHQMGTHSAFIEEEVVGRE 185
+K IRERLD I EK F++ P I+ +S THS+ +E V+GR+
Sbjct: 121 RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRM-RMSSSRETHSSLNKERVIGRD 180
Query: 186 DDKMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVS 245
DDK +K LL D NVS IAIVGMGGIGKTTLA+ LY+D +VS+HF++KMW+WVS
Sbjct: 181 DDKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVS 240
Query: 246 DQFDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEF 305
QF K VV++++E A+ P + ++ + +LQ++IGGKKYLLVMD++ +ESE +W++
Sbjct: 241 KQFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKL 300
Query: 306 KPLLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK----KIG 365
K LL+ G RGSKI+ITKRD V +E+ MT ++L+GLS++SSWSLF++VAF+ K
Sbjct: 301 KDLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPI 360
Query: 366 SPKMIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKN-DELSRITRENNDLTNI 425
+PK+ +GKEI CGG+PL IRH+GR L SK EEEW+S + + + ++NND+ +I
Sbjct: 361 NPKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASI 420
Query: 426 LALSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYF 485
L LSY LP HLKQCFAYSSL+ K + +ELIRQW+A GF++S +G KSME I +YF
Sbjct: 421 LELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYF 480
Query: 486 TELCWRFFYE------HHDGYVCMHDKMHDLA-----------GMVTHN----ENIYHFS 545
ELCWRFFYE + D YV MHD M++LA G + +N E HFS
Sbjct: 481 DELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFS 540
Query: 546 LGHNMKAWKDFPSELLEAKGLRTLR-----KSLLNFMDKVDLAKLLSNFPRLRVLDLSNA 605
L + +++W+D S+L +AKGLRT N +++ +L +L S+FPRLRVL L +
Sbjct: 541 LDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGS 600
Query: 606 NIYMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDL 665
NI+++P ++KKLK LRYLDLS N ++SLPN I EL +LQTL L GC L E+PRDI L
Sbjct: 601 NIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKL 660
Query: 666 ANLRHLELDGRSKLTHM-PKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYI 725
NLRHL+ S++THM P+ + KLSCLQT+SLFV D K +KL EL EL +L G L I
Sbjct: 661 VNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFD-CTKTNKLRELNELNNLTGELKI 720
Query: 726 KGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESILEGLELHPNVE 785
GL+ LR PYE L NLK KK LR L L+WK+ E + DE +++GLE +PNVE
Sbjct: 721 TGLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGLYKHEDE---ADERVMKGLEPNPNVE 780
Query: 786 SVCIWRYKGQELPNWVSNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQFI 845
S+ I Y G LP W+ N KLT I + C+RLQ LPQFH L LK L L +L SL+FI
Sbjct: 781 SLGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFI 840
Query: 846 IDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMK 905
+NP SSS +FFPSL+ L L NM NLE WWE +
Sbjct: 841 DKFDNPFSSS----MFFPSLKSLCLANMANLEGWWE----------------------LS 900
Query: 906 GVLGDEESEKRRDLLPNFARLTDLSIHDCPKLRWVPRQPSLKK--VELNKVGVWMVTS-I 955
+ E SE + L P F L L I+ CPKL +P+ PS K V L VGV +V S I
Sbjct: 901 RAVAGETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTI 959
BLAST of Sed0015318 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 409.5 bits (1051), Expect = 8.3e-114
Identity = 306/980 (31.22%), Postives = 497/980 (50.71%), Query Frame = 0
Query: 37 EDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLGEALYDADDVLDEISTQALRRQ 96
E+ L+RL + + I AVL DAEE+Q + +++ W+ +L + +Y A+D LD+I+T+ALR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 97 VMTRGKHAKQVRIFFSRSNQPTF----NYKMSREIKIIRERLDEISAEKDKFGFIE-TSS 156
+ + ++R R + F + + ++ + RL+ ++++++ G E T+
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 157 TPRMIQHDGLSHQMGTHSAFIEEEVVGREDDKMLVKRLLFDIDDVAGHNVSFIAIVGMGG 216
P+ ++ T S E EV GR+DDK + R L ++ + ++ +AIVG+GG
Sbjct: 156 IPK--------QRLPTTSLVDESEVFGRDDDKDEIMRFLIP-ENGKDNGITVVAIVGIGG 215
Query: 217 IGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVKRVVEEMIELASGSQPKVKGIEALCV 276
+GKTTL++LLY+D V +F K+W VS++FDV ++ +++ E + + ++ L V
Sbjct: 216 VGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQV 275
Query: 277 KLQELIGGK--KYLLVMDDMWDESEKKWEEFKPLLMCGARGSKILITKRDSIVLAEVRSM 336
KL+E + G +LLV+DD+W+E+ W+ + + A+GS+IL+T R V A +
Sbjct: 276 KLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRV-ASIMCA 335
Query: 337 TKLISLEGLSDESSWSLFQKVAF---KKIGSPKMIQLGKEILGKCGGIPLVIRHIGRLLF 396
+ +L+ LSD WSLF K F + + ++ L + I+ KC G+PL ++ +G +L
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 397 SKFSEEEWISFKNDELSRITRENNDLTNILALSYKHLPPHLKQCFAYSSLYSKYEFVDKD 456
+ EW + + + + ++L +L +SY +LP HLK+CFAY S++ K +KD
Sbjct: 396 FEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKD 455
Query: 457 ELIRQWIAHGFVQSREGSKSM-EIANDYFTELCWRFFYEHHDGYVCMHDKMHDLAGMVT- 516
+++ W+A GF+Q SK++ E+ N+YF+EL R + MHD +++LA +
Sbjct: 456 KVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQFASG 515
Query: 517 --------------HNENIYHFSLGHNMKAWKDFPSELLEAKGLRT-LRKSLLN-----F 576
Y L N +F + L E K LRT L SL N
Sbjct: 516 EFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA-LREVKFLRTFLPLSLTNSSRSCC 575
Query: 577 MDKVDLAKLLSNFPRLRVLDLSNANI-YMVPKALKKLKHLRYLDLSNNLGLQSLPNFIGE 636
+D++ KLL RLRVL LS+ I + P K + H R+LDLS L+ LP +
Sbjct: 576 LDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCY 635
Query: 637 LVNLQTLYLNGCKTLTELPRDIIDLANLRHLELDGRSKLTHMPKEIGKLSCLQTLSLFVL 696
+ NLQTL L+ C +L ELP DI +L NLR+L+L G +KL MP+ G+L LQTL+ F +
Sbjct: 636 MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 695
Query: 697 DEKKKGSKLSELKELIDLRGGLYIKGLEHLRGNPYEAKLANLKDKKGLRDLVLQWKVRSK 756
GS++SEL L DL G L I L+ + + +A ANL KK LR++ W+ S
Sbjct: 696 -SASDGSRISELGGLHDLHGKLKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSS 755
Query: 757 ADESKDG----LNDESILEGLELHPNVESVCIWRYKGQELPNWVSNP-ISKLTHISLGKC 816
+ E+ N+ + E L H ++E + I RYKG+ P+W+S+P S++ I L +C
Sbjct: 756 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 815
Query: 817 NRLQRLPQFHQLPALKKLHLEELTSLQ-----FIIDNNNPGSSSSSTFVFFPSLEILILK 876
LP QLP LK+LH+ + LQ F + F SLE L
Sbjct: 816 QYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQP---FRSLETLRFD 875
Query: 877 NMPNLEAWWEGESRVRDGKSKKTGLLPDLKEWMKGVLGDEESEKRRDLLPNF-ARLTDLS 936
N+P+ + W D + + L P LK+ L + LP F L L
Sbjct: 876 NLPDWQEW-------LDVRVTRGDLFPSLKK-----LFILRCPELTGTLPTFLPSLISLH 935
Query: 937 IHDCPKLRWVPRQPSLKKVELNKVGVWMVTSIGPSSNLEYVTLKNIEDLKHIPMDLRRSS 973
I+ C L + P NL+ +++K+ D + L +
Sbjct: 936 IYKCGLLDFQPDHHEYS-----------------YRNLQTLSIKSSCDTL-VKFPLNHFA 967
BLAST of Sed0015318 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 394.0 bits (1011), Expect = 3.6e-109
Identity = 298/936 (31.84%), Postives = 471/936 (50.32%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQL-SSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKS 65
MA L++ A ++ ++ +SQ L +L LKRLK + VLADA++R
Sbjct: 1 MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60
Query: 66 KLLKDWLKKLGEALYDADDVLDEISTQALRRQVMTR--GKHAKQVRIFFSR-SNQPTFNY 125
+ +K WL + +A + A+D+LDE+ T+ALRR+V+ G + R + Q
Sbjct: 61 REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIEP 120
Query: 126 KMSREIKIIRERLDEISAEKDKFGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGRED 185
KM + ++++ + I + G E S T + + +VGR +
Sbjct: 121 KMEKVVRLLEHHVKHI----EVIGLKEYSETR---EPQWRQASRSRPDDLPQGRLVGRVE 180
Query: 186 DKMLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSD 245
DK+ + LL D+++ + I++VGM G+GKTTL ++++D V++HF++KMW+
Sbjct: 181 DKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGI 240
Query: 246 QFDVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFK 305
F+V V + +++ + S + + +L ++L++ + GK++LLV+DD W ES+ +WE F+
Sbjct: 241 NFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQ 300
Query: 306 PLLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFKKIGSPKMI 365
GSKI++T R IV + V K+ ++ +++E W L + AF I +
Sbjct: 301 VAFTDAEEGSKIVLTTRSEIV-STVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSIN 360
Query: 366 Q----LGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILA 425
Q +GK I +C G+PL R I L SK + ++W + + + N + +L
Sbjct: 361 QELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN----FSSYTNSILPVLK 420
Query: 426 LSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSME-IANDYFTE 485
LSY LPP LK+CFA S++ K D++EL+ W+A + S+ +E I NDY +
Sbjct: 421 LSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGNDYLGD 480
Query: 486 LCWRFFYEHHD---GYVCMHDKMHDLAGMVT-------HNENI-------YHFSLGHNMK 545
L + F++ D MHD M+DLA V+ ++NI HFS +
Sbjct: 481 LVAQSFFQRLDITMTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQC 540
Query: 546 AWKDFPSELLEAKGLRTL-------RKSLLNFMDKVDLAKLLSNFPRLRVLDLSNANIYM 605
+ A+ LRT+ L +KV L LL+ LR+L LS+ I
Sbjct: 541 DASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKV-LNPLLNALSGLRILSLSHYQITN 600
Query: 606 VPKALKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLR 665
+PK+LK LK LRYLDLS+ ++ LP F+ L NLQTL L+ C+ LT LP+ I +L NLR
Sbjct: 601 LPKSLKGLKLLRYLDLSST-KIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLR 660
Query: 666 HLELDGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIKGLEH 725
L+L G + L MP I KL LQ LS FV+ + G+ L ELKEL LRG L I L++
Sbjct: 661 LLDLVG-TPLVEMPPGIKKLRSLQKLSNFVIG-RLSGAGLHELKELSHLRGTLRISELQN 720
Query: 726 LRGNPYEAKLANLKDKKGLRDLVLQWKVRSK----ADESKDGLNDESILEGLELHPNVES 785
+ EAK A LK K L L+L+W V+ + + + +L LE HP++++
Sbjct: 721 V-AFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKT 780
Query: 786 VCIWRYKGQELPNWV-SNPISKLTHISLGKCNRLQRLPQFHQLPALKKLHLEELTSLQ-- 845
CI Y+G P W+ + +T ++L CN LP QLP+LK L +E+ LQ
Sbjct: 781 FCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKV 840
Query: 846 ----FIIDNNNPGSSSSSTFVFFPSLEILILKNMPNLEAWWEGESRVRDGKSKKTGLLPD 898
F +NN+ G V F SL+IL MP + W E + G+ P
Sbjct: 841 GLDFFFGENNSRG-------VPFQSLQILKFYGMPRWDEWICPE--------LEDGIFPC 900
BLAST of Sed0015318 vs. TAIR 10
Match:
AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 263.5 bits (672), Expect = 7.3e-70
Identity = 237/811 (29.22%), Postives = 376/811 (46.36%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIG-VEDDLKRLKSNVSAIQAVLADAEERQNKS 65
M + V+T F L L++ G + L+ L+S + +Q+ L DAE ++ +
Sbjct: 1 MVDAVVTVFLEKTL-----NILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTN 60
Query: 66 KLLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMS 125
+ L+ + L E +Y+A+D+L + R +A R+ +R YK S
Sbjct: 61 ETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPAR---VPLQYKKS 120
Query: 126 REIKIIRERLDEISAEKDK-FGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDK 185
+ ++ I ER+ +I ++ + F FI S+ R D S + H+ +VVG E DK
Sbjct: 121 KRLQEINERITKIKSQVEPYFEFITPSNVGRDNGTDRWSSPVYDHT-----QVVGLEGDK 180
Query: 186 MLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQF 245
+K LF +D + +A VGMGG+GKTT+A+ +++D E+ F+ ++WV VS F
Sbjct: 181 RKIKEWLFRSND---SQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTF 240
Query: 246 DVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPL 305
++++ ++ G I L K+Q+ + GK+YL+VMDD+WD++ W++
Sbjct: 241 TEEQIMRSILR-NLGDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQG 300
Query: 306 LMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK----KIGSPK 365
L G GS I+ T+ +S+ K E LS ++SW LF VAF P+
Sbjct: 301 LPRGQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPE 360
Query: 366 MIQLGKEILGKCGGIPLVIRHIGRLLFSK-FSEEEW---ISFKNDELSRITRENNDLTNI 425
+ +GKEI+ KC G+PL I+ +G LL K EW DEL T E +++ +
Sbjct: 361 LEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSS 420
Query: 426 LALSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFT 485
L LSY LP HLK C SLY + + K +L+ WI GFV R G + E D F+
Sbjct: 421 LQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATESGEDCFS 480
Query: 486 ELCWRFFYEHHDG------YVC-MHDKMHDLAGMVTHNE--------NIYHFSLGHNMKA 545
L R E D C +HD + DL + + N H + N
Sbjct: 481 GLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFDE 540
Query: 546 WKDFPSELLEAKGLRTLRKSLLNFMDKVDLAKLLSNFPRLRVLDLS----NANIYMVPKA 605
+ + L + T + +N ++ DLAK ++ LRVLD+S +A + +
Sbjct: 541 KQIKVNHKLRGV-VSTTKTGEVNKLNS-DLAKKFTDCKYLRVLDISKSIFDAPLSEILDE 600
Query: 606 LKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRHLEL 665
+ L+HL L LSN L P + +L NLQ L + C+ L +L I+ L L++
Sbjct: 601 IASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDM 660
Query: 666 DGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIKGLEHLRGN 725
L PK IG L L+ L F G KLSE+K L +LR GL RG+
Sbjct: 661 TNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRK----LGLSLTRGD 720
Query: 726 PYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESI--LEGLELHPNVESVCIWRY 783
E ++ L L+ K+ S + D D+ I ++ L + + + Y
Sbjct: 721 QIE--------EEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFY 780
BLAST of Sed0015318 vs. TAIR 10
Match:
AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 263.5 bits (672), Expect = 7.3e-70
Identity = 237/811 (29.22%), Postives = 376/811 (46.36%), Query Frame = 0
Query: 6 MAEGVLTNFAGNVLAQLSSQALQQLGMVIG-VEDDLKRLKSNVSAIQAVLADAEERQNKS 65
M + V+T F L L++ G + L+ L+S + +Q+ L DAE ++ +
Sbjct: 1 MVDAVVTVFLEKTL-----NILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTN 60
Query: 66 KLLKDWLKKLGEALYDADDVLDEISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMS 125
+ L+ + L E +Y+A+D+L + R +A R+ +R YK S
Sbjct: 61 ETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPAR---VPLQYKKS 120
Query: 126 REIKIIRERLDEISAEKDK-FGFIETSSTPRMIQHDGLSHQMGTHSAFIEEEVVGREDDK 185
+ ++ I ER+ +I ++ + F FI S+ R D S + H+ +VVG E DK
Sbjct: 121 KRLQEINERITKIKSQVEPYFEFITPSNVGRDNGTDRWSSPVYDHT-----QVVGLEGDK 180
Query: 186 MLVKRLLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQF 245
+K LF +D + +A VGMGG+GKTT+A+ +++D E+ F+ ++WV VS F
Sbjct: 181 RKIKEWLFRSND---SQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTF 240
Query: 246 DVKRVVEEMIELASGSQPKVKGIEALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFKPL 305
++++ ++ G I L K+Q+ + GK+YL+VMDD+WD++ W++
Sbjct: 241 TEEQIMRSILR-NLGDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQG 300
Query: 306 LMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFK----KIGSPK 365
L G GS I+ T+ +S+ K E LS ++SW LF VAF P+
Sbjct: 301 LPRGQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPE 360
Query: 366 MIQLGKEILGKCGGIPLVIRHIGRLLFSK-FSEEEW---ISFKNDELSRITRENNDLTNI 425
+ +GKEI+ KC G+PL I+ +G LL K EW DEL T E +++ +
Sbjct: 361 LEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSS 420
Query: 426 LALSYKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFT 485
L LSY LP HLK C SLY + + K +L+ WI GFV R G + E D F+
Sbjct: 421 LQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATESGEDCFS 480
Query: 486 ELCWRFFYEHHDG------YVC-MHDKMHDLAGMVTHNE--------NIYHFSLGHNMKA 545
L R E D C +HD + DL + + N H + N
Sbjct: 481 GLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFDE 540
Query: 546 WKDFPSELLEAKGLRTLRKSLLNFMDKVDLAKLLSNFPRLRVLDLS----NANIYMVPKA 605
+ + L + T + +N ++ DLAK ++ LRVLD+S +A + +
Sbjct: 541 KQIKVNHKLRGV-VSTTKTGEVNKLNS-DLAKKFTDCKYLRVLDISKSIFDAPLSEILDE 600
Query: 606 LKKLKHLRYLDLSNNLGLQSLPNFIGELVNLQTLYLNGCKTLTELPRDIIDLANLRHLEL 665
+ L+HL L LSN L P + +L NLQ L + C+ L +L I+ L L++
Sbjct: 601 IASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDM 660
Query: 666 DGRSKLTHMPKEIGKLSCLQTLSLFVLDEKKKGSKLSELKELIDLRGGLYIKGLEHLRGN 725
L PK IG L L+ L F G KLSE+K L +LR GL RG+
Sbjct: 661 TNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRK----LGLSLTRGD 720
Query: 726 PYEAKLANLKDKKGLRDLVLQWKVRSKADESKDGLNDESI--LEGLELHPNVESVCIWRY 783
E ++ L L+ K+ S + D D+ I ++ L + + + Y
Sbjct: 721 QIE--------EEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFY 780
BLAST of Sed0015318 vs. TAIR 10
Match:
AT3G46730.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 220.3 bits (560), Expect = 7.1e-57
Identity = 201/739 (27.20%), Postives = 342/739 (46.28%), Query Frame = 0
Query: 16 GNVLAQLSSQALQQLGMVIGVEDDLKRLKSNVSAIQAVLADAEERQNKSKLLKDWLKKLG 75
G VL ++ + ++ ++GV+DDL+ LK+ ++ I L D E R+ + ++ K+W K +
Sbjct: 7 GFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKLVL 66
Query: 76 EALYDADDVLD----EISTQALRRQVMTRGKHAKQVRIFFSRSNQPTFNYKMSREIKIIR 135
+ YD +DVLD ++ ++LRR ++ ++ + Y + +I+ ++
Sbjct: 67 DIAYDIEDVLDTYFLKLEERSLRRGLLR----------LTNKIGKKRDAYNIVEDIRTLK 126
Query: 136 ERLDEISAEKDKFGFIETSSTPRMIQHDGLSHQMGTHSAFIEEE--VVGREDD-KMLVKR 195
R+ +I+ +++ FG I + + PR + + + +++E VVG EDD K+L+ +
Sbjct: 127 RRILDITRKRETFG-IGSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVK 186
Query: 196 LLFDIDDVAGHNVSFIAIVGMGGIGKTTLARLLYHDCEVSKHFDLKMWVWVSDQFDVK-- 255
LL D + + I+I GMGG+GKT LAR LY+ +V + FD + W +VS ++ +
Sbjct: 187 LLSDNEKDKSY---IISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDI 246
Query: 256 --RVVEEMIELASGSQPKVKGI---EALCVKLQELIGGKKYLLVMDDMWDESEKKWEEFK 315
R++ + +++ K+K E L V L L+ GK Y++V+DD+WD WE K
Sbjct: 247 LIRIIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDPD--AWESLK 306
Query: 316 PLLMCGARGSKILITKRDSIVLAEVRSMTKLISLEGLSDESSWSLFQKVAFKKIG--SPK 375
L C RGSK++IT R + V L L+ E SW+LF++ AF I
Sbjct: 307 RALPCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEKVDED 366
Query: 376 MIQLGKEILGKCGGIPLVIRHIGRLLFSKFSEEEWISFKNDELSRITRENNDLTNILALS 435
+ + GKE++ KCGG+PL I + LL S+ EW R+ + ++ + LS
Sbjct: 367 LQRTGKEMVKKCGGLPLAIVVLSGLL-SRKRTNEWHEVCASLWRRLKDNSIHISTVFDLS 426
Query: 436 YKHLPPHLKQCFAYSSLYSKYEFVDKDELIRQWIAHGFVQSREGSKSMEIANDYFTELCW 495
+K + LK CF Y S++ + + ++LI +A GF+Q E ++A Y EL
Sbjct: 427 FKEMRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCYIDELVD 486
Query: 496 RFF-----YEHHDGYVC-MHDKMHDLA-------------------------GMVTHNEN 555
R E C +HD + DLA +V H N
Sbjct: 487 RSLVKAERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVVHHLMN 546
Query: 556 IYHFSLGHNMKAWKDF------------PSELLEAKGLRTLRKSLLNFMDK---VDLAKL 615
Y+ K + F + L+ K LR L L F+ K L +
Sbjct: 547 DYYLCDRRVNKRMRSFLFIGERRGFGYVNTTNLKLKLLRVLNMEGLLFVSKNISNTLPDV 606
Query: 616 LSNFPRLRVLDLSNANIYMVPKALKKLKHLRYLDLSNNLGLQSLPN-------------- 674
+ LR L +++ + ++P ++ L+ L+ LD S N Q +
Sbjct: 607 IGELIHLRYLGIADTYVSILPASISNLRFLQTLDASGNDPFQYTTDLSKLTSLRHVIGKF 666
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022953917.1 | 2.6e-258 | 53.20 | putative disease resistance protein RGA4 [Cucurbita moschata] | [more] |
XP_023547878.1 | 1.7e-257 | 52.90 | putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo] | [more] |
XP_038896095.1 | 1.6e-252 | 50.50 | putative disease resistance protein RGA4 [Benincasa hispida] | [more] |
XP_031745107.1 | 3.9e-251 | 50.45 | putative disease resistance protein RGA4 [Cucumis sativus] | [more] |
KAE8646395.1 | 3.9e-251 | 50.45 | hypothetical protein Csa_016171 [Cucumis sativus] | [more] |
Match Name | E-value | Identity | Description | |
Q7XA40 | 9.8e-128 | 33.50 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XA39 | 1.9e-123 | 32.10 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XA42 | 2.3e-121 | 31.65 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XBQ9 | 3.7e-119 | 31.40 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
Q9LRR4 | 1.2e-112 | 31.22 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GPK9 | 1.2e-258 | 53.20 | putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
A0A6J1JVS7 | 2.8e-250 | 53.19 | putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC11148... | [more] |
A0A6J1D168 | 8.9e-233 | 49.54 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A1S3CEH4 | 3.6e-226 | 47.58 | disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=... | [more] |
A0A5A7UWP6 | 8.4e-223 | 47.43 | Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |