Sed0014583 (gene) Chayote v1

Overview
NameSed0014583
Typegene
OrganismSechium edule (Chayote v1)
DescriptionEukaryotic aspartyl protease family protein
LocationLG08: 2979424 .. 2982679 (-)
RNA-Seq ExpressionSed0014583
SyntenySed0014583
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGACGATGCTATCCTTTCCCCTGCAAACTCAACCAATATTATGTGAGTCTTGTCATTAGTATTTCTCTATGATTTGATGCTTATATTTTATACCGTCATAGATCACATTTCATCTATGAATCAGTTTCTATAATCTTCATCTTATATTAATGTGAGGCTTGTCATTTTTCCTTTGCCCATGACCTTGTTATGAACTCATACTTTAATATTTCTGCCCCAATCTTTTACCTGCTCGTCTTTCTTGTTCTACATAGCTTTGACATGATAATTGATATATATTATGCATACAACTGTTCTGAAAAACCTACACAAACATCAGTTTCAACATGGTGATTCTGCATCATCTTCTGCCTGTCCTAACCTTGGCATTTTATTTGTCAACAGCAATAACCTCGCCCACCACGATCACGACAAAGCCGTCACGTTTGGCAACCAAGCTCATCCATCGCAACTCTTATCTACATCCACTCTACGATCCAAACGAGACCGTTGAAGATCGAACTAAGAGAGAGCAGACGAGTTCATTTAAACGGTTTACTTATCTCGAGTCCAAGATTAAAGAACTAAACTCTATTGGCGATGTGGCTCAATCAAATCTCATCCCTTTCAATCAAGGTAGCGGGTTTCTCGTTAATTTGTCGATTGGTTTGCCGCCCGTGGCGCAGCTTCTAGCAGTGGACACTGGTAGCTACCTATTGTGGGTGCAATGTTTGCCTTGTGTGGAGTGTTTTAGGCAAAGGAACTCATGGTTCGATCCTTCGAAATCGACAAGCTTCGAAGTATTGGGGTGTGGCTTTCCTGGGTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATGAAGCCGAGTACAAGTTGAGGTACCTTGGTGGGGATACCTCTGAAGGAATTCTTGCTAAGGAATCACTTACCTTTGACACATCTGATGAAGGTACTTCTCAATATAATGCTTCTTCAATCCCCAACAAATAAGGGAAGATTTAAAAAAAAAACTAATTCTGAAAAAAACAACTTTTCTTCAAAAATATTTATAAAGTAGATTGAAAAGTAAGGAATTTTATAAACGAAAATAGTATTTGTAGGTTAAATTTTTTAAAAGTATTATCAAATGAGGTTATTTTTCGTTCCGTATATAATAAATGTTTGAATTTTAAAAATGTCAAATGGAAATATAACTTTTAAGTTTATGTTTAATCTATTTTAATACATTGTTGAATTAAAAAAAAATCTCAACATATTTATAAACTTGATGAATTATAGAATTTTCTAGCTTGCCTACAAGCTTTGTAAATTTTTTAAAAAATAAATGCCAAATAAATTAAAATTGAAAGTAACAGCTGCCGTGATTTACAAGTAACGGCTAGCACAATTGCGAGCACGGTCACCGATTGGCTCAATGGGCTTTTTGGTAAAAACATGATCAAGTTTTTTCGTTTTTTCTTTATTACTTTTTTTTTTTTTTTTTGAAGAAATTTTTTCTTTATTACTTATTTGCATTTAAAGGTGGAGAATTTTTCGAATTCGTAATTTCTTCATACGTCACATCATAGGATTCTTTTCAGTTTAAACATTATTTTGACTTGATGTACCAAAATAGTTTATTAATTTATTAACCTTTTTTTTTTGCAAGATAGATTTTTTTTTTTTTTTTTTTTTTTTTGATTAGAAGATCTATTTTGTTAAGTTAATTCAAATACTCACAATTTTTTTTTTACTTATATAATTGATCAAATTTAGAAAATAATTATAAATATGATTACTTAAAAAACTTTAACAACCAATCGTTGGTATTACTAAGTATTTATACTTTTTTTTACAATATTGTATTAATAATAAATGAGTATTTATAATACACTTCTAAACTGTATCAGTAAATATAAATATAAATACTTTAAAATTGTACTGCATTATAGTGAGGGAATTTTGCATGTGACAAAATATTTACACTTATAACATAAAATCAAAATTAATTCATTTGTAAAAAAAATTCAAATTGAGTTTTATCCGTCATTAGTGATAGATGTCAATCACTTATTAGTGATAGATGTCTATCACTAATTGAGTTCTATCACTAATTATTAATAGATGTCTAATGTGCTATACATTCAATTATTTTAGAAATTGTGCTACAAAATTAATACAAGATGTAGAATTTGGTGCTGAGCGCAAATTCCCTTATAATGATGTGTAATTTCTTTTTTCAAAAAATAATAAAACATTTTTTCCTAATTTTATAACCATGTCCACGTATATTTATATATATTTTTCTTACAAAAAAACAAAAATAATTTCATTACTTTTATCCCTAATGATAATGATGATGATAATGATAGTAAACAATAATTACAACATGAATGAAACTCCGAACAGGCAGAATCACAAAGGCGAATTTGACATTCGGGTGCGGCCACATGAACAAGAAAACCAACAGAGACGACAGTTACAACGGTATCTTGGGACTTGGCGGATACCCTCACATAACAATGGCCACTCAACTGGGCAACAAATTCTCGTACTGCATAGGCGACATTACCGACCCTTTCTACCCCCACAACACCCTCATCTTAGGCAATGGGGCCTTCACCCAAGGCGACTCGACCCCCATGCACATCCTCTTCGGCCACTACTACGTCACCCTACAGGCCATCAGCCTTGGCTCCAACACCCTCGACATCGACCCACGTGTCTTCCGCTTCACCGGCCACGGCTCGGGCGGGGTCCTCATCGACTCCGGCATGACCTACACCCGCCTTGCCAAGCCCGGGTTCGAGGCTCTTTGCGATGGCATTGTTGATTTGATGATGGGGTTGCTGGAGCGGATCCCGACTAGGAGGGACTTTGAAGGGTTGTGTTATAGAGGGGTCTTGGGTCGGGACCTTGTCGGGTTTCCGGCTGTGACTTTTCATTTTGATGGGGGTGCTGATTTGGTGTTGGAGCCTGGGAGCTTGTTTCGGCAGCATGGTCCGGATCGCTTCTGTTTGGCTGTGTTGCCTAGTGATTCCGAGATGTTGGATTTGTCTGTGATTGGGATTTTGGCTCAGCAGAATTATAATGTGGGGTTTGATCTTGAACAAATGAGGGTGTTCTTCTGTAGGATTGATTGTCAGCTTCTTCTTGACTAACTGCCCATGACAACAATAATAGTTCATAGCTCAATTGGCATAAGTGTGTTTGTTTGTATAAGTTTCAAAATCTCCAACATTTCAAATAAAGGGCATGCACTTTTTTGCAGATTTACAAATTTGGTTCAGAT

mRNA sequence

CGGACGATGCTATCCTTTCCCCTGCAAACTCAACCAATATTATCAATAACCTCGCCCACCACGATCACGACAAAGCCGTCACGTTTGGCAACCAAGCTCATCCATCGCAACTCTTATCTACATCCACTCTACGATCCAAACGAGACCGTTGAAGATCGAACTAAGAGAGAGCAGACGAGTTCATTTAAACGGTTTACTTATCTCGAGTCCAAGATTAAAGAACTAAACTCTATTGGCGATGTGGCTCAATCAAATCTCATCCCTTTCAATCAAGGTAGCGGGTTTCTCGTTAATTTGTCGATTGGTTTGCCGCCCGTGGCGCAGCTTCTAGCAGTGGACACTGGTAGCTACCTATTGTGGGTGCAATGTTTGCCTTGTGTGGAGTGTTTTAGGCAAAGGAACTCATGGTTCGATCCTTCGAAATCGACAAGCTTCGAAGTATTGGGGTGTGGCTTTCCTGGGTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATGAAGCCGAGTACAAGTTGAGGTACCTTGGTGGGGATACCTCTGAAGGAATTCTTGCTAAGGAATCACTTACCTTTGACACATCTGATGAAGGCAGAATCACAAAGGCGAATTTGACATTCGGGTGCGGCCACATGAACAAGAAAACCAACAGAGACGACAGTTACAACGGTATCTTGGGACTTGGCGGATACCCTCACATAACAATGGCCACTCAACTGGGCAACAAATTCTCGTACTGCATAGGCGACATTACCGACCCTTTCTACCCCCACAACACCCTCATCTTAGGCAATGGGGCCTTCACCCAAGGCGACTCGACCCCCATGCACATCCTCTTCGGCCACTACTACGTCACCCTACAGGCCATCAGCCTTGGCTCCAACACCCTCGACATCGACCCACGTGTCTTCCGCTTCACCGGCCACGGCTCGGGCGGGGTCCTCATCGACTCCGGCATGACCTACACCCGCCTTGCCAAGCCCGGGTTCGAGGCTCTTTGCGATGGCATTGTTGATTTGATGATGGGGTTGCTGGAGCGGATCCCGACTAGGAGGGACTTTGAAGGGTTGTGTTATAGAGGGGTCTTGGGTCGGGACCTTGTCGGGTTTCCGGCTGTGACTTTTCATTTTGATGGGGGTGCTGATTTGGTGTTGGAGCCTGGGAGCTTGTTTCGGCAGCATGGTCCGGATCGCTTCTGTTTGGCTGTGTTGCCTAGTGATTCCGAGATGTTGGATTTGTCTGTGATTGGGATTTTGGCTCAGCAGAATTATAATGTGGGGTTTGATCTTGAACAAATGAGGGTGTTCTTCTGTAGGATTGATTGTCAGCTTCTTCTTGACTAACTGCCCATGACAACAATAATAGTTCATAGCTCAATTGGCATAAGTGTGTTTGTTTGTATAAGTTTCAAAATCTCCAACATTTCAAATAAAGGGCATGCACTTTTTTGCAGATTTACAAATTTGGTTCAGAT

Coding sequence (CDS)

ATGCTATCCTTTCCCCTGCAAACTCAACCAATATTATCAATAACCTCGCCCACCACGATCACGACAAAGCCGTCACGTTTGGCAACCAAGCTCATCCATCGCAACTCTTATCTACATCCACTCTACGATCCAAACGAGACCGTTGAAGATCGAACTAAGAGAGAGCAGACGAGTTCATTTAAACGGTTTACTTATCTCGAGTCCAAGATTAAAGAACTAAACTCTATTGGCGATGTGGCTCAATCAAATCTCATCCCTTTCAATCAAGGTAGCGGGTTTCTCGTTAATTTGTCGATTGGTTTGCCGCCCGTGGCGCAGCTTCTAGCAGTGGACACTGGTAGCTACCTATTGTGGGTGCAATGTTTGCCTTGTGTGGAGTGTTTTAGGCAAAGGAACTCATGGTTCGATCCTTCGAAATCGACAAGCTTCGAAGTATTGGGGTGTGGCTTTCCTGGGTATAACTACATTAGTGGTTACAAATGCAATGGTTTTAATGAAGCCGAGTACAAGTTGAGGTACCTTGGTGGGGATACCTCTGAAGGAATTCTTGCTAAGGAATCACTTACCTTTGACACATCTGATGAAGGCAGAATCACAAAGGCGAATTTGACATTCGGGTGCGGCCACATGAACAAGAAAACCAACAGAGACGACAGTTACAACGGTATCTTGGGACTTGGCGGATACCCTCACATAACAATGGCCACTCAACTGGGCAACAAATTCTCGTACTGCATAGGCGACATTACCGACCCTTTCTACCCCCACAACACCCTCATCTTAGGCAATGGGGCCTTCACCCAAGGCGACTCGACCCCCATGCACATCCTCTTCGGCCACTACTACGTCACCCTACAGGCCATCAGCCTTGGCTCCAACACCCTCGACATCGACCCACGTGTCTTCCGCTTCACCGGCCACGGCTCGGGCGGGGTCCTCATCGACTCCGGCATGACCTACACCCGCCTTGCCAAGCCCGGGTTCGAGGCTCTTTGCGATGGCATTGTTGATTTGATGATGGGGTTGCTGGAGCGGATCCCGACTAGGAGGGACTTTGAAGGGTTGTGTTATAGAGGGGTCTTGGGTCGGGACCTTGTCGGGTTTCCGGCTGTGACTTTTCATTTTGATGGGGGTGCTGATTTGGTGTTGGAGCCTGGGAGCTTGTTTCGGCAGCATGGTCCGGATCGCTTCTGTTTGGCTGTGTTGCCTAGTGATTCCGAGATGTTGGATTTGTCTGTGATTGGGATTTTGGCTCAGCAGAATTATAATGTGGGGTTTGATCTTGAACAAATGAGGGTGTTCTTCTGTAGGATTGATTGTCAGCTTCTTCTTGACTAA

Protein sequence

MLSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQMRVFFCRIDCQLLLD
Homology
BLAST of Sed0014583 vs. NCBI nr
Match: XP_031742409.1 (aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_020065 [Cucumis sativus])

HSP 1 Score: 713.0 bits (1839), Expect = 1.6e-201
Identity = 339/442 (76.70%), Postives = 383/442 (86.65%), Query Frame = 0

Query: 2   LSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFK 61
           L+F L T    +I S T ITTKPSRLATKLIHRNSYLHPLYD NETVEDR+KREQTSS +
Sbjct: 17  LAFYLST----AIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSIE 76

Query: 62  RFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQC 121
           RF +LESKIKEL S+G+ A+S+LIPFN+GSGFLVNLSIG PPV QL+ VDTGS LLWVQC
Sbjct: 77  RFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQC 136

Query: 122 LPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEG 181
           LPC+ CF+Q  SWFDP KS SF+ LGCGFPGYNYI+GYKCN FN+AEYKLRYLGGD+S+G
Sbjct: 137 LPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGDSSQG 196

Query: 182 ILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNK 241
           ILAKESL F+T DEG+I K+N+TFGCGHMN KTN DD+YNG+ GLG YPHITMATQLGNK
Sbjct: 197 ILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLGNK 256

Query: 242 FSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRV 301
           FSYCIGDI +P Y HN L+LG G++ +GDSTP+ I FGHYYVTLQ+IS+GS TL IDP  
Sbjct: 257 FSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISVGSKTLKIDPNA 316

Query: 302 FRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVL 361
           F+ +  GSGGVLIDSGMTYT+LA  GFE L D IVDLM GLLERIPT+R FEGLC++GV+
Sbjct: 317 FKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVV 376

Query: 362 GRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQN 421
            RDLVGFPAVTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SE+L+LSVIGILAQQN
Sbjct: 377 SRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQN 436

Query: 422 YNVGFDLEQMRVFFCRIDCQLL 444
           YNVGFDLEQM+VFF RIDCQLL
Sbjct: 437 YNVGFDLEQMKVFFRRIDCQLL 454

BLAST of Sed0014583 vs. NCBI nr
Match: XP_022134792.1 (probable aspartic protease At2g35615 [Momordica charantia])

HSP 1 Score: 713.0 bits (1839), Expect = 1.6e-201
Identity = 337/434 (77.65%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 13  SITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKE 72
           ++ S T +TTKPSRL TKLIHRNSYLHPLYDPNETVEDR+KRE+TSS +RF YLESKIKE
Sbjct: 24  AVISSTDVTTKPSRLVTKLIHRNSYLHPLYDPNETVEDRSKREETSSTERFAYLESKIKE 83

Query: 73  LNSI-GDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQR 132
           LNS+ G+ A++NLIPFNQGSGFLVN SIG PPV QL  VDTGS LLWVQCLPCV CFRQ 
Sbjct: 84  LNSVGGNEARANLIPFNQGSGFLVNFSIGQPPVTQLAVVDTGSSLLWVQCLPCVNCFRQS 143

Query: 133 NSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTFD 192
            SWFDP KS SF++L C FPG+NY+ GYKCN F++AEYKLRYLGGDTSEGILAKESL F+
Sbjct: 144 GSWFDPLKSWSFKILDCDFPGHNYVRGYKCNDFHQAEYKLRYLGGDTSEGILAKESLLFE 203

Query: 193 TSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDITD 252
           T DEG+I KANLTFGCGHMN KTNRDD+YNG+ GLGGYPHITMATQLGNKFSYCIGDITD
Sbjct: 204 TPDEGKIRKANLTFGCGHMNTKTNRDDTYNGVFGLGGYPHITMATQLGNKFSYCIGDITD 263

Query: 253 PFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSGG 312
           P Y HN L LG+GAF +GDSTP+ I+FGHYYV+L+ IS+GS  L IDP  F+ T  G GG
Sbjct: 264 PLYAHNHLFLGHGAFIEGDSTPLQIIFGHYYVSLEGISVGSKRLKIDPNAFQMTSDGRGG 323

Query: 313 VLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAV 372
           VLIDSGMTYT+L   GFE L D I DLM G+LERIPTRR FEGLCY+GV+GRDLVG P V
Sbjct: 324 VLIDSGMTYTKLTNGGFELLFDEIADLMKGVLERIPTRRKFEGLCYKGVVGRDLVGLPPV 383

Query: 373 TFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQM 432
           TFHF GGADLVLE GSLFRQHG DRFCLAVLPS+SEM++LSVIG+LAQQNYNVGFDLEQM
Sbjct: 384 TFHFAGGADLVLESGSLFRQHGGDRFCLAVLPSNSEMMNLSVIGVLAQQNYNVGFDLEQM 443

Query: 433 RVFFCRIDCQLLLD 446
           +VFF RIDCQLL D
Sbjct: 444 KVFFRRIDCQLLGD 457

BLAST of Sed0014583 vs. NCBI nr
Match: XP_023515827.1 (aspartic proteinase CDR1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712.6 bits (1838), Expect = 2.1e-201
Identity = 332/435 (76.32%), Postives = 379/435 (87.13%), Query Frame = 0

Query: 11  ILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKI 70
           I++I S     TKPSRLAT+LIHRNSYLHPLYDPNETVEDR+KRE+TSS +RF YLESKI
Sbjct: 41  IIAIISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKI 100

Query: 71  KELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQ 130
           KEL S+G+VA+SNL PFN+GSGFLVNLSIG PPVAQL+ +DTGS LLWVQCLPC+ CFRQ
Sbjct: 101 KELKSVGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQ 160

Query: 131 RNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTF 190
            +SWFDP KS+SF++LGCGFPGYNY+SGY+CNG+N+AEYKLRYLGGDTS+G+LAKESL F
Sbjct: 161 SSSWFDPLKSSSFKILGCGFPGYNYVSGYRCNGYNQAEYKLRYLGGDTSQGLLAKESLLF 220

Query: 191 DTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDIT 250
           +TSDEG+I KANLTFGCGHMN KTN DDSYNG+ GLG YP+ITMATQLGNKFSYCIGDI 
Sbjct: 221 ETSDEGKIRKANLTFGCGHMNFKTNIDDSYNGVFGLGAYPYITMATQLGNKFSYCIGDIN 280

Query: 251 DPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSG 310
           DP Y HN L+LG GA+ +GDSTP+ I FGHYYV L+ IS+G+  L+IDP+ F+ T  G G
Sbjct: 281 DPLYTHNQLVLGEGAYVEGDSTPLEIHFGHYYVNLEGISVGTKRLNIDPKAFQMTWDGRG 340

Query: 311 GVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPA 370
           GVLIDSGMTYT+LA  GFE L D I+DL  GLLERIPTRR FEGLC++GV+ RDL+G P 
Sbjct: 341 GVLIDSGMTYTKLANGGFELLYDEILDLANGLLERIPTRRQFEGLCFKGVVSRDLIGLPP 400

Query: 371 VTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQ 430
           VTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SEML+LSVIGILAQQNYNV FDLEQ
Sbjct: 401 VTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEMLNLSVIGILAQQNYNVAFDLEQ 460

Query: 431 MRVFFCRIDCQLLLD 446
           M+VFF RIDCQLL D
Sbjct: 461 MKVFFSRIDCQLLDD 475

BLAST of Sed0014583 vs. NCBI nr
Match: XP_023515828.1 (probable aspartic protease At2g35615 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711.1 bits (1834), Expect = 6.1e-201
Identity = 329/424 (77.59%), Postives = 374/424 (88.21%), Query Frame = 0

Query: 22  TKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQ 81
           TKPSRLAT+LIHRNSYLHPLYDPNETVEDR+KRE+TSS +RF YLESKIKEL S+G+VA+
Sbjct: 5   TKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKIKELKSVGNVAR 64

Query: 82  SNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKST 141
           SNL PFN+GSGFLVNLSIG PPVAQL+ +DTGS LLWVQCLPC+ CFRQ +SWFDP KS+
Sbjct: 65  SNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQSSSWFDPLKSS 124

Query: 142 SFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTFDTSDEGRITKA 201
           SF++LGCGFPGYNY+SGY+CNG+N+AEYKLRYLGGDTS+G+LAKESL F+TSDEG+I KA
Sbjct: 125 SFKILGCGFPGYNYVSGYRCNGYNQAEYKLRYLGGDTSQGLLAKESLLFETSDEGKIRKA 184

Query: 202 NLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDITDPFYPHNTLIL 261
           NLTFGCGHMN KTN DDSYNG+ GLG YP+ITMATQLGNKFSYCIGDI DP Y HN L+L
Sbjct: 185 NLTFGCGHMNFKTNIDDSYNGVFGLGAYPYITMATQLGNKFSYCIGDINDPLYTHNQLVL 244

Query: 262 GNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSGGVLIDSGMTYT 321
           G GA+ +GDSTP+ I FGHYYV L+ IS+G+  L+IDP+ F+ T  G GGVLIDSGMTYT
Sbjct: 245 GEGAYVEGDSTPLEIHFGHYYVNLEGISVGTKRLNIDPKAFQMTWDGRGGVLIDSGMTYT 304

Query: 322 RLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAVTFHFDGGADL 381
           +LA  GFE L D I+DL  GLLERIPTRR FEGLC++GV+ RDL+G P VTFHF GGADL
Sbjct: 305 KLANGGFELLYDEILDLANGLLERIPTRRQFEGLCFKGVVSRDLIGLPPVTFHFAGGADL 364

Query: 382 VLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQMRVFFCRIDCQ 441
           VLE GSLFRQHG DRFCLA+LPS+SEML+LSVIGILAQQNYNV FDLEQM+VFF RIDCQ
Sbjct: 365 VLESGSLFRQHGGDRFCLAILPSNSEMLNLSVIGILAQQNYNVAFDLEQMKVFFSRIDCQ 424

Query: 442 LLLD 446
           LL D
Sbjct: 425 LLDD 428

BLAST of Sed0014583 vs. NCBI nr
Match: XP_038878960.1 (aspartic proteinase CDR1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-200
Identity = 335/442 (75.79%), Postives = 378/442 (85.52%), Query Frame = 0

Query: 2   LSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFK 61
           L+F L T    +I S    TTKPSRLATKLIHRNSYLHPLYDP ET+EDR+KRE+TSS +
Sbjct: 17  LAFYLST----AIISSMAFTTKPSRLATKLIHRNSYLHPLYDPTETIEDRSKREETSSIE 76

Query: 62  RFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQC 121
           RF YLESKIKEL S+G+ A+S+LIPFNQGSGFLVNLSIG PPV QL+  DTGS LLWVQC
Sbjct: 77  RFAYLESKIKELKSVGNEARSSLIPFNQGSGFLVNLSIGSPPVTQLVVADTGSSLLWVQC 136

Query: 122 LPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEG 181
           LPC++CFRQ NSWFDP KSTSF++LGCGF GYNYISGY+CNGFN+AEYKLRYLGGDTS+G
Sbjct: 137 LPCIDCFRQSNSWFDPLKSTSFKILGCGFAGYNYISGYRCNGFNQAEYKLRYLGGDTSQG 196

Query: 182 ILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNK 241
           +LAKESL F+T DEG+I K NLTFGCGHMN KTN DD+YNG+ GLG YP+ITMATQLGNK
Sbjct: 197 VLAKESLLFETLDEGKIKKTNLTFGCGHMNSKTNNDDTYNGVFGLGAYPYITMATQLGNK 256

Query: 242 FSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRV 301
           FSYCIGDI +P Y HN L+LG G++ +GDSTP+ I FGHYYVTLQ IS+GS  L IDP+ 
Sbjct: 257 FSYCIGDINNPVYTHNHLVLGEGSYIEGDSTPLQIHFGHYYVTLQGISVGSKRLKIDPKA 316

Query: 302 FRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVL 361
           F+ T  G GGVLIDSGMTYT+LA  GFE L D I+DLM GLLERIPT R FEGLC++GV+
Sbjct: 317 FQMTWDGRGGVLIDSGMTYTKLANGGFELLYDEILDLMTGLLERIPTERKFEGLCFKGVV 376

Query: 362 GRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQN 421
            RDL+GFP VTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SEML+LSVIGILAQQN
Sbjct: 377 SRDLIGFPTVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEMLNLSVIGILAQQN 436

Query: 422 YNVGFDLEQMRVFFCRIDCQLL 444
           YNV FDLEQM+VFF RIDCQLL
Sbjct: 437 YNVAFDLEQMKVFFRRIDCQLL 454

BLAST of Sed0014583 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 127/438 (29.00%), Postives = 214/438 (48.86%), Query Frame = 0

Query: 24  PSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQSN 83
           P   + +LIHR+S L P+Y+P  TV DR       S  R      ++ + +      QS 
Sbjct: 23  PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTD-----LQSG 82

Query: 84  LIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSF 143
           LI       F ++++IG PP+      DTGS L WVQC PC +C+++    FD  KS+++
Sbjct: 83  LI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTY 142

Query: 144 EVLGCGFPGYNYISGYK--CNGFNE-AEYKLRYLGGDTSEGILAKESLTFDTSDEGRITK 203
           +   C       +S  +  C+  N   +Y+  Y     S+G +A E+++ D++    ++ 
Sbjct: 143 KSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSF 202

Query: 204 ANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGN----KFSYCIGDITDPFYPH 263
               FGCG+ N  T  D++ +GI+GLGG  H+++ +QLG+    KFSYC+   +      
Sbjct: 203 PGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLGSSISKKFSYCLSHKSATTNGT 262

Query: 264 NTLILGNGAFTQGDSTPMHIL---------FGHYYVTLQAISLGSNTL-----DIDPRVF 323
           + + LG  +     S    ++           +YY+TL+AIS+G   +       +P   
Sbjct: 263 SVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDD 322

Query: 324 RFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLG 383
                 SG ++IDSG T T L    F+     + + + G  +R+   +     C++   G
Sbjct: 323 GILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTG-AKRVSDPQGLLSHCFKS--G 382

Query: 384 RDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNY 441
              +G P +T HF  GAD+ L P + F +   D  CL+++P+     ++++ G  AQ ++
Sbjct: 383 SAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPT----TEVAIYGNFAQMDF 442

BLAST of Sed0014583 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-44
Identity = 139/428 (32.48%), Postives = 200/428 (46.73%), Query Frame = 0

Query: 31  LIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQSNLIPFNQG 90
           LIHR+S   P Y+P ET   R +     S  R  +   K        D      I     
Sbjct: 35  LIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEK--------DNTPQPQIDLTSN 94

Query: 91  SG-FLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSFEVLGCG 150
           SG +L+N+SIG PP   +   DTGS LLW QC PC +C+ Q +  FDP  S++++ + C 
Sbjct: 95  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCS 154

Query: 151 FPGYNYISGY-KCN-GFNEAEYKLRYLGGDTSEGILAKESLTFDTSDEGRITKANLTFGC 210
                 +     C+   N   Y L Y     ++G +A ++LT  +SD   +   N+  GC
Sbjct: 155 SSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGC 214

Query: 211 GHMNKKT-NRDDSYNGILGLGGYPHITMATQLGN----KFSYCIGDITDPFYPHNTLILG 270
           GH N  T N+  S  GI+GLGG P +++  QLG+    KFSYC+  +T      + +  G
Sbjct: 215 GHNNAGTFNKKGS--GIVGLGGGP-VSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFG 274

Query: 271 NGAFTQGD---STPMHILFGH---YYVTLQAISLGSNTLDIDPRVFRFTGHGS----GGV 330
             A   G    STP+         YY+TL++IS+GS  +       +++G  S    G +
Sbjct: 275 TNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQI-------QYSGSDSESSEGNI 334

Query: 331 LIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAVT 390
           +IDSG T T L    +  L D +   +    ++ P  +    LCY      DL   P +T
Sbjct: 335 IIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDP--QSGLSLCYSAT--GDL-KVPVIT 394

Query: 391 FHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQMR 441
            HFD GAD+ L+  + F Q   D  C A   S S     S+ G +AQ N+ VG+D     
Sbjct: 395 MHFD-GADVKLDSSNAFVQVSEDLVCFAFRGSPS----FSIYGNVAQMNFLVGYDTVSKT 434

BLAST of Sed0014583 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.8e-44
Identity = 116/382 (30.37%), Postives = 185/382 (48.43%), Query Frame = 0

Query: 67  ESKIKELNSIGDVAQSNLIPFNQGSG-FLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCV 126
           E +++ +N++   +     P   G G +L+N++IG P  +    +DTGS L+W QC PC 
Sbjct: 69  ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCT 128

Query: 127 ECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAK 186
           +CF Q    F+P  S+SF  L C       +    CN  NE +Y   Y  G T++G +A 
Sbjct: 129 QCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNN-NECQYTYGYGDGSTTQGYMAT 188

Query: 187 ESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLG-NKFSY 246
           E+ TF+TS     +  N+ FGCG  N+   + +   G++G+G  P +++ +QLG  +FSY
Sbjct: 189 ETFTFETS-----SVPNIAFGCGEDNQGFGQGNG-AGLIGMGWGP-LSLPSQLGVGQFSY 248

Query: 247 CIGDITDPFYPHNTLILGNGAFTQGDSTPMHILF------GHYYVTLQAISLGSNTLDID 306
           C+          +TL LG+ A    + +P   L        +YY+TLQ I++G + L I 
Sbjct: 249 CMTSYGSS--SPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIP 308

Query: 307 PRVFRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYR 366
              F+    G+GG++IDSG T T L +  + A+     D +   L  +         C++
Sbjct: 309 SSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQ 368

Query: 367 GVLGRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILA 426
                  V  P ++  FDGG  L L   ++         CLA+    S  L +S+ G + 
Sbjct: 369 QPSDGSTVQVPEISMQFDGGV-LNLGEQNILISPAEGVICLAM--GSSSQLGISIFGNIQ 428

Query: 427 QQNYNVGFDLEQMRVFFCRIDC 441
           QQ   V +DL+ + V F    C
Sbjct: 429 QQETQVLYDLQNLAVSFVPTQC 435

BLAST of Sed0014583 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-39
Identity = 115/356 (32.30%), Postives = 170/356 (47.75%), Query Frame = 0

Query: 93  FLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSFEVLGCGFPG 152
           +L+NLSIG P       +DTGS L+W QC PC +CF Q    F+P  S+SF  L C    
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154

Query: 153 YNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTFDTSDEGRITKANLTFGCGHMNK 212
              +S   C+  N  +Y   Y  G  ++G +  E+LTF     G ++  N+TFGCG  N+
Sbjct: 155 CQALSSPTCSN-NFCQYTYGYGDGSETQGSMGTETLTF-----GSVSIPNITFGCGENNQ 214

Query: 213 KTNRDDSYNGILGLGGYPHITMATQLG-NKFSYCIGDITDPFYPHNTLILGNGAFTQGDS 272
              + +   G++G+G  P +++ +QL   KFSYC+  I     P N L+LG+ A +    
Sbjct: 215 GFGQGNG-AGLVGMGRGP-LSLPSQLDVTKFSYCMTPIGSS-TPSN-LLLGSLANSVTAG 274

Query: 273 TPMHILF------GHYYVTLQAISLGSNTLDIDPRVFRF-TGHGSGGVLIDSGMTYTRLA 332
           +P   L         YY+TL  +S+GS  L IDP  F   + +G+GG++IDSG T T   
Sbjct: 275 SPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFV 334

Query: 333 KPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAVTFHFDGGADLVLE 392
              ++++    +  +   L  +        LC++       +  P    HFDGG DL L 
Sbjct: 335 NNAYQSVRQEFISQIN--LPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELP 394

Query: 393 PGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQMRVFFCRIDC 441
             + F        CLA+  S   M   S+ G + QQN  V +D     V F    C
Sbjct: 395 SENYFISPSNGLICLAMGSSSQGM---SIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Sed0014583 vs. ExPASy Swiss-Prot
Match: Q9SV77 (Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.4e-38
Identity = 114/361 (31.58%), Postives = 169/361 (46.81%), Query Frame = 0

Query: 89  QGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQR-NSWFDPSKSTSFEVLG 148
           +G  F+  +  G P   Q L +DTGS L W QC PC +C+ Q+    + P+ S ++    
Sbjct: 54  RGLAFMAEIHFGSPQKKQFLHMDTGSSLTWTQCFPCSDCYAQKIYPKYRPAASITYRDAM 113

Query: 149 CGFPGYNYISGYKCNGFNE-AEYKLRYLGGDTSEGILAKESLTFDTSDEGRITKANLTFG 208
           C          +  +       Y+  YL     +G LA+E +T DT D G      + FG
Sbjct: 114 CEDSHPKSNPHFAFDPLTRICTYQQHYLDETNIKGTLAQEMITVDTHDGGFKRVHGVYFG 173

Query: 209 CGHMNKKTNRDDSY---NGILGLGGYPHITMATQLGNKFSYCIGDITDPFYPHNTLILGN 268
           C      T  D SY    GILGL G    ++  + G+KFS+C+G+I++P   HN LILG+
Sbjct: 174 C-----NTLSDGSYFTGTGILGL-GVGKYSIIGEFGSKFSFCLGEISEPKASHN-LILGD 233

Query: 269 GAFTQGDSTPMHILFGHYYVTLQAISLGSN-TLDIDPRVFRFTGHGSGGVLIDSGMTYTR 328
           GA  QG  T ++I  GH    L++I +G   TLD   +VF           +D+G T + 
Sbjct: 234 GANVQGHPTVINITEGHTIFQLESIIVGEEITLDDPVQVF-----------VDTGSTLSH 293

Query: 329 LAKPGFEALCDGIVDLMMGL-LERIPTRRDFEGLCYRGVLGRDLVGFPAVTFHFDGGADL 388
           L+   +    D   DL+    L   PT      LCY+      L     V F FD GA+L
Sbjct: 294 LSTNLYYKFVDAFDDLIGSRPLSYEPT------LCYKADTIERLEKMD-VGFKFDVGAEL 353

Query: 389 VLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQMRVFFCRIDCQ 443
            +   ++F Q GP       + ++ E     +IG++A Q YNVG+DL     +  + DC 
Sbjct: 354 SVNIHNIFIQQGPPEIRCLAIQNNKESFSHVIIGVIAMQGYNVGYDLSAKTAYINKQDCD 389

BLAST of Sed0014583 vs. ExPASy TrEMBL
Match: A0A0A0LUP5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G424350 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.8e-202
Identity = 339/442 (76.70%), Postives = 383/442 (86.65%), Query Frame = 0

Query: 2   LSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFK 61
           L+F L T    +I S T ITTKPSRLATKLIHRNSYLHPLYD NETVEDR+KREQTSS +
Sbjct: 17  LAFYLST----AIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSIE 76

Query: 62  RFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQC 121
           RF +LESKIKEL S+G+ A+S+LIPFN+GSGFLVNLSIG PPV QL+ VDTGS LLWVQC
Sbjct: 77  RFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQC 136

Query: 122 LPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEG 181
           LPC+ CF+Q  SWFDP KS SF+ LGCGFPGYNYI+GYKCN FN+AEYKLRYLGGD+S+G
Sbjct: 137 LPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGDSSQG 196

Query: 182 ILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNK 241
           ILAKESL F+T DEG+I K+N+TFGCGHMN KTN DD+YNG+ GLG YPHITMATQLGNK
Sbjct: 197 ILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLGNK 256

Query: 242 FSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRV 301
           FSYCIGDI +P Y HN L+LG G++ +GDSTP+ I FGHYYVTLQ+IS+GS TL IDP  
Sbjct: 257 FSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISVGSKTLKIDPNA 316

Query: 302 FRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVL 361
           F+ +  GSGGVLIDSGMTYT+LA  GFE L D IVDLM GLLERIPT+R FEGLC++GV+
Sbjct: 317 FKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVV 376

Query: 362 GRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQN 421
            RDLVGFPAVTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SE+L+LSVIGILAQQN
Sbjct: 377 SRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQN 436

Query: 422 YNVGFDLEQMRVFFCRIDCQLL 444
           YNVGFDLEQM+VFF RIDCQLL
Sbjct: 437 YNVGFDLEQMKVFFRRIDCQLL 454

BLAST of Sed0014583 vs. ExPASy TrEMBL
Match: A0A6J1C0L1 (probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006977 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.8e-202
Identity = 337/434 (77.65%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 13  SITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKE 72
           ++ S T +TTKPSRL TKLIHRNSYLHPLYDPNETVEDR+KRE+TSS +RF YLESKIKE
Sbjct: 24  AVISSTDVTTKPSRLVTKLIHRNSYLHPLYDPNETVEDRSKREETSSTERFAYLESKIKE 83

Query: 73  LNSI-GDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQR 132
           LNS+ G+ A++NLIPFNQGSGFLVN SIG PPV QL  VDTGS LLWVQCLPCV CFRQ 
Sbjct: 84  LNSVGGNEARANLIPFNQGSGFLVNFSIGQPPVTQLAVVDTGSSLLWVQCLPCVNCFRQS 143

Query: 133 NSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTFD 192
            SWFDP KS SF++L C FPG+NY+ GYKCN F++AEYKLRYLGGDTSEGILAKESL F+
Sbjct: 144 GSWFDPLKSWSFKILDCDFPGHNYVRGYKCNDFHQAEYKLRYLGGDTSEGILAKESLLFE 203

Query: 193 TSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDITD 252
           T DEG+I KANLTFGCGHMN KTNRDD+YNG+ GLGGYPHITMATQLGNKFSYCIGDITD
Sbjct: 204 TPDEGKIRKANLTFGCGHMNTKTNRDDTYNGVFGLGGYPHITMATQLGNKFSYCIGDITD 263

Query: 253 PFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSGG 312
           P Y HN L LG+GAF +GDSTP+ I+FGHYYV+L+ IS+GS  L IDP  F+ T  G GG
Sbjct: 264 PLYAHNHLFLGHGAFIEGDSTPLQIIFGHYYVSLEGISVGSKRLKIDPNAFQMTSDGRGG 323

Query: 313 VLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPAV 372
           VLIDSGMTYT+L   GFE L D I DLM G+LERIPTRR FEGLCY+GV+GRDLVG P V
Sbjct: 324 VLIDSGMTYTKLTNGGFELLFDEIADLMKGVLERIPTRRKFEGLCYKGVVGRDLVGLPPV 383

Query: 373 TFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQM 432
           TFHF GGADLVLE GSLFRQHG DRFCLAVLPS+SEM++LSVIG+LAQQNYNVGFDLEQM
Sbjct: 384 TFHFAGGADLVLESGSLFRQHGGDRFCLAVLPSNSEMMNLSVIGVLAQQNYNVGFDLEQM 443

Query: 433 RVFFCRIDCQLLLD 446
           +VFF RIDCQLL D
Sbjct: 444 KVFFRRIDCQLLGD 457

BLAST of Sed0014583 vs. ExPASy TrEMBL
Match: A0A5D3DZ20 (Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.7e-201
Identity = 340/442 (76.92%), Postives = 381/442 (86.20%), Query Frame = 0

Query: 2   LSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFK 61
           L+F L T  ILS    T+I TKPSRLATKLIHRNSYLHPLYDPNETVEDR+KREQ SS +
Sbjct: 406 LAFYLSTAIILS----TSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIE 465

Query: 62  RFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQC 121
           RF +LESKIKEL S+G+ A+S+LIPFN+GSGFLVNLSIG PPV QL+ VDTGS LLWVQC
Sbjct: 466 RFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQC 525

Query: 122 LPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEG 181
           LPC+ CF+Q  SWFDP KS SF+ LGCGFPGYNYI+GYKCNG N+AEYKLRYLGGD+S+G
Sbjct: 526 LPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYKLRYLGGDSSQG 585

Query: 182 ILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNK 241
           ILAKESL F+T DEG+I K NLTFGCGHMN KTN DD+YNG+ GLG YPHITMATQLGNK
Sbjct: 586 ILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGNK 645

Query: 242 FSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRV 301
           FSYCIGDI +P Y HN L+LG G++ +GDSTP+ I FGHYYVTLQ IS+GS TL IDP  
Sbjct: 646 FSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISVGSKTLKIDPNA 705

Query: 302 FRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVL 361
           F+ +  GSGGVLIDSGMTYT+LA  GFE L D IVDLM GLLERIPT+R FEGLC++GV+
Sbjct: 706 FKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVV 765

Query: 362 GRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQN 421
            RDLVGFPAVTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SE+L+LSVIGILAQQN
Sbjct: 766 SRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQN 825

Query: 422 YNVGFDLEQMRVFFCRIDCQLL 444
           YNVGFDLEQM+VFF RIDCQLL
Sbjct: 826 YNVGFDLEQMKVFFRRIDCQLL 842

BLAST of Sed0014583 vs. ExPASy TrEMBL
Match: A0A5A7UU11 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005510 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.7e-201
Identity = 340/442 (76.92%), Postives = 382/442 (86.43%), Query Frame = 0

Query: 2   LSFPLQTQPILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFK 61
           L+F L T  ILS    T+ITTKPSRLATKLIHRNSYLHPLYDPNETVEDR+KREQ SS +
Sbjct: 406 LAFYLSTAIILS----TSITTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIE 465

Query: 62  RFTYLESKIKELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQC 121
           RF +LESKIKEL S+G+ A+S+LIPFN+GSGFLVNLSIG PPV QL+ VDTGS LLWVQC
Sbjct: 466 RFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQC 525

Query: 122 LPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEG 181
           LPC+ CF+Q  SWFDP KS SF+ LGCGFPGYNYI+GYKCNG N+AEYKLRYLGGD+S+G
Sbjct: 526 LPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYKLRYLGGDSSQG 585

Query: 182 ILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNK 241
           ILAKESL F+T DEG+I K NLTFGCGHMN KTN DD+YNG+ GLG YP+ITMATQLGNK
Sbjct: 586 ILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPYITMATQLGNK 645

Query: 242 FSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRV 301
           FSYCIGDI +P Y HN L+LG G++ +GDSTP+ I FGHYYVTLQ IS+GS TL IDP  
Sbjct: 646 FSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISVGSKTLKIDPNA 705

Query: 302 FRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVL 361
           F+ +  GSGGVLIDSGMTYT+LA  GFE L D IVDLM GLLERIPT+R FEGLC++GV+
Sbjct: 706 FKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVV 765

Query: 362 GRDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQN 421
            RDLVGFPAVTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SE+L+LSVIGILAQQN
Sbjct: 766 SRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQN 825

Query: 422 YNVGFDLEQMRVFFCRIDCQLL 444
           YNVGFDLEQM+VFF RIDCQLL
Sbjct: 826 YNVGFDLEQMKVFFRRIDCQLL 842

BLAST of Sed0014583 vs. ExPASy TrEMBL
Match: A0A6J1E195 (probable aspartic protease At2g35615 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429882 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.1e-200
Identity = 332/435 (76.32%), Postives = 377/435 (86.67%), Query Frame = 0

Query: 11  ILSITSPTTITTKPSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKI 70
           I++I S     TKPSRLAT+LIHRNSYLHPLYDPNETVEDR+KRE+TSS +RF YLESKI
Sbjct: 41  IITIISSMMTMTKPSRLATRLIHRNSYLHPLYDPNETVEDRSKREETSSIERFAYLESKI 100

Query: 71  KELNSIGDVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQ 130
           KEL SIG+VA+SNL PFN+GSGFLVNLSIG PPVAQL+ +DTGS LLWVQCLPC+ CFRQ
Sbjct: 101 KELKSIGNVARSNLSPFNRGSGFLVNLSIGSPPVAQLVVIDTGSSLLWVQCLPCINCFRQ 160

Query: 131 RNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLGGDTSEGILAKESLTF 190
            +SWFDP KS+SF++LGCGF GYNY+SGYKCNG+N+AEYKLRYLGGDTS+G+LAKESL F
Sbjct: 161 SSSWFDPLKSSSFKILGCGFRGYNYVSGYKCNGYNQAEYKLRYLGGDTSQGLLAKESLLF 220

Query: 191 DTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGNKFSYCIGDIT 250
           +TSDEG+I K NLTFGCGHMN KTN DDSYNG+ GLG YP+ITMATQLGNKFSYCIGDI 
Sbjct: 221 ETSDEGKIRKTNLTFGCGHMNFKTNIDDSYNGVFGLGAYPYITMATQLGNKFSYCIGDIN 280

Query: 251 DPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAISLGSNTLDIDPRVFRFTGHGSG 310
           DP Y HN L+LG GA+ +GDSTP+ I FGHYYV L+ IS+G+  L+IDP+ F+ T  G G
Sbjct: 281 DPLYTHNQLVLGEGAYVEGDSTPLEIHFGHYYVNLEGISVGTKRLNIDPKAFQMTWDGRG 340

Query: 311 GVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLVGFPA 370
           GVLIDSGMTYT+LA  GFE L D I+DL  GLLERIPTRR FEGLC++GV+ RDL+G P 
Sbjct: 341 GVLIDSGMTYTKLANGGFELLYDEILDLANGLLERIPTRRQFEGLCFKGVVSRDLIGLPP 400

Query: 371 VTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGFDLEQ 430
           VTFHF GGADLVLE GSLFRQHG DRFCLA+LPS+SEML+LSVIGILAQQNYNV FDLEQ
Sbjct: 401 VTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEMLNLSVIGILAQQNYNVAFDLEQ 460

Query: 431 MRVFFCRIDCQLLLD 446
           M+VFF RIDCQLL D
Sbjct: 461 MKVFFSRIDCQLLDD 475

BLAST of Sed0014583 vs. TAIR 10
Match: AT4G30030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 315.5 bits (807), Expect = 7.0e-86
Identity = 169/395 (42.78%), Postives = 235/395 (59.49%), Query Frame = 0

Query: 51  RTKREQTSSFKRFTYLESKIKELNSIGDV-AQSNLIPFNQGSGFLVNLSIGLPPVAQLLA 110
           RTK +++S  K   YL SK    + + ++   S++ P    + FL N+SIG PPV QLL 
Sbjct: 36  RTKTQESSKIK-IGYLHSKSTPASRLDNLWTVSHVTPIPNPAAFLANISIGNPPVPQLLL 95

Query: 111 VDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEY 170
           +DTGS L W+ CLPC +C+ Q   +F PS+S+++    C    +     ++       +Y
Sbjct: 96  IDTGSDLTWIHCLPC-KCYPQTIPFFHPSRSSTYRNASCVSAPHAMPQIFRDEKTGNCQY 155

Query: 171 KLRYLGGDTSEGILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGY 230
            LRY     + GILA+E LTF+TSD+G I+K N+ FGCG  N    +   Y+G+LGLG  
Sbjct: 156 HLRYRDFSNTRGILAEEKLTFETSDDGLISKQNIVFGCGQDNSGFTK---YSGVLGLGPG 215

Query: 231 PHITMATQLGNKFSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGHYYVTLQAIS 290
               +    G+KFSYC G +T+P YPHN LILGNGA  +GD TP+ I    YY+ LQAIS
Sbjct: 216 TFSIVTRNFGSKFSYCFGSLTNPTYPHNILILGNGAKIEGDPTPLQIFQDRYYLDLQAIS 275

Query: 291 LGSNTLDIDPRVFRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTR 350
            G   LDI+P  F+      GG +ID+G + T LA+  +E L + I  L+  +L R+   
Sbjct: 276 FGEKLLDIEPGTFQ-RYRSQGGTVIDTGCSPTILAREAYETLSEEIDFLLGEVLRRVKDW 335

Query: 351 RDFEGLCYRGVLGRDLVGFPAVTFHFDGGADLVLEPGSLF-RQHGPDRFCLAVLPSDSEM 410
             +   CY G L  DL GFP VTFHF GGA+L L+  SLF      D FCLA+  +  + 
Sbjct: 336 DQYTTPCYEGNLKLDLYGFPVVTFHFAGGAELALDVESLFVSSESGDSFCLAMTMNTFD- 395

Query: 411 LDLSVIGILAQQNYNVGFDLEQMRVFFCRIDCQLL 444
            D+SVIG +AQQNYNVG++L  M+V+F R DC+++
Sbjct: 396 -DMSVIGAMAQQNYNVGYNLRTMKVYFQRTDCEII 422

BLAST of Sed0014583 vs. TAIR 10
Match: AT2G23945.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.0e-78
Identity = 184/459 (40.09%), Postives = 256/459 (55.77%), Query Frame = 0

Query: 7   QTQPILSITSPTTITT---KPSRLATKLIHRNSY--LHPLYDPNETVEDRTKREQTSSFK 66
           QT  +L IT    + T   KP+R+A KLIHR S   L+P      T ED  K     S  
Sbjct: 6   QTSLLLFITVSYFVVTESIKPNRMAMKLIHRESVARLNPNARVPITPEDHIKHLTDISSA 65

Query: 67  RFTYLESKI-KELNSIG---DVAQSNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLL 126
           RF YL++ I KEL S     DV Q+      + S FLVN S+G PPV QL  +DTGS LL
Sbjct: 66  RFKYLQNSIDKELGSSNFQVDVEQA-----IKTSLFLVNFSVGQPPVPQLTIMDTGSSLL 125

Query: 127 WVQCLPCVECFRQR--NSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLG 186
           W+QC PC  C      +  F+P+ S++F    C      Y     C   N+  Y+  Y+ 
Sbjct: 126 WIQCQPCKHCSSDHMIHPVFNPALSSTFVECSCDDRFCRYAPNGHCGSSNKCVYEQVYIS 185

Query: 187 GDTSEGILAKESLTFDTSDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMA 246
           G  S+G+LAKE LTF T +   +    + FGCG+ N +   +  + GILGLG  P  ++A
Sbjct: 186 GTGSKGVLAKERLTFTTPNGNTVVTQPIAFGCGYENGE-QLESHFTGILGLGAKP-TSLA 245

Query: 247 TQLGNKFSYCIGDITDPFYPHNTLILGNGAFTQGDSTPMHILFGH--YYVTLQAISLGSN 306
            QLG+KFSYCIGD+ +  Y +N L+LG  A   GD TP+     +  YY+ L+ IS+G  
Sbjct: 246 VQLGSKFSYCIGDLANKNYGYNQLVLGEDADILGDPTPIEFETENSIYYMNLEGISVGDT 305

Query: 307 TLDIDPRVFRFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFE 366
            L+I+P VF+  G  + GV++DSG  YT LA   +  L + I  ++   LER    RDF 
Sbjct: 306 QLNIEPVVFKRRGPRT-GVILDSGTLYTWLADIAYRELYNEIKSILDPKLERF-WFRDF- 365

Query: 367 GLCYRGVLGRDLVGFPAVTFHFDGGADLVLEPGSLF----RQHGPDRFCLAVLPS---DS 426
            LCY G +  +L+GFP VTFHF GGA+L +E  S+F      +  + FC++V P+     
Sbjct: 366 -LCYHGRVSEELIGFPVVTFHFAGGAELAMEATSMFYPLSEPNTFNVFCMSVKPTKEHGG 425

Query: 427 EMLDLSVIGILAQQNYNVGFDLEQMRVFFCRIDCQLLLD 446
           E  + + IG++AQQ YN+G+DL++  ++  RIDC  L D
Sbjct: 426 EYKEFTAIGLMAQQYYNIGYDLKEKNIYLQRIDCVQLDD 453

BLAST of Sed0014583 vs. TAIR 10
Match: AT4G30040.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-72
Identity = 164/388 (42.27%), Postives = 218/388 (56.19%), Query Frame = 0

Query: 58  SSFKRFTYLESKIKELNSIGDVAQ--SNLIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSY 117
           +S +R  YL++K     + GD+    S  +P      FLVN+SIG PP+ QLL +DT S 
Sbjct: 54  ASVERLEYLKAK-----TTGDIIAHLSPNVPIIP-QAFLVNISIGSPPITQLLHMDTASD 113

Query: 118 LLWVQCLPCVECFRQRNSWFDPSKSTSFEVLGCGFPGYNYISGYKCNGFNEAEYKLRYLG 177
           LLW+QCLPC+ C+ Q    FDPS+S +     C    Y+  S          EY +RY+ 
Sbjct: 114 LLWIQCLPCINCYAQSLPIFDPSRSYTHRNETCRTSQYSMPSLKFNANTRSCEYSMRYVD 173

Query: 178 GDTSEGILAKESLTFDT--SDEGRITKANLTFGCGHMNKKTNRDDSYNGILGLGGYPHIT 237
              S+GILA+E L F+T   +       ++ FGCGH N          GILGL GY   +
Sbjct: 174 DTGSKGILAREMLLFNTIYDESSSAALHDVVFGCGHDN--YGEPLVGTGILGL-GYGEFS 233

Query: 238 MATQLGNKFSYCIGDITDPFYPHNTLILG-NGAFTQGDSTPMHILFGHYYVTLQAISLGS 297
           +  + G KFSYC G + DP YPHN L+LG +GA   GD+TP+ I  G YYVT++AIS+  
Sbjct: 234 LVHRFGKKFSYCFGSLDDPSYPHNVLVLGDDGANILGDTTPLEIHNGFYYVTIEAISVDG 293

Query: 298 NTLDIDPRVF-RFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRD 357
             L IDPRVF R    G GG +ID+G + T L +  ++ L + I D+  G        +D
Sbjct: 294 IILPIDPRVFNRNHQTGLGGTIIDTGNSLTSLVEEAYKPLKNRIEDIFEGRFTAADVSQD 353

Query: 358 --FEGLCYRGVLGRDLV--GFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSE 417
              +  CY G   RDLV  GFP VTFHF  GA+L L+  SLF +  P+ FCLAV P    
Sbjct: 354 DMIKMECYNGNFERDLVESGFPIVTFHFSEGAELSLDVKSLFMKLSPNVFCLAVTPG--- 413

Query: 418 MLDLSVIGILAQQNYNVGFDLEQMRVFF 436
             +L+ IG  AQQ+YN+G+DLE M V F
Sbjct: 414 --NLNSIGATAQQSYNIGYDLEAMEVSF 427

BLAST of Sed0014583 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 192.6 bits (488), Expect = 6.8e-49
Identity = 131/434 (30.18%), Postives = 216/434 (49.77%), Query Frame = 0

Query: 27  LATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQSNLIP 86
           L  +LIHR+S   PLY+P+ TV DR       S  R     +K           QS LI 
Sbjct: 29  LTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRFTTKTD--------LQSGLI- 88

Query: 87  FNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSFEVL 146
            + G  + +++SIG PP       DTGS L WVQC PC +C++Q +  FD  KS++++  
Sbjct: 89  -SNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQNSPLFDKKKSSTYKTE 148

Query: 147 GCGFPGYNYISGYKCNGFNEAE--YKLRYLGGDTS--EGILAKESLTFDTSDEGRITKAN 206
            C       +S ++  G +E++   K RY  GD S  +G +A E+++ D+S    ++   
Sbjct: 149 SCDSKTCQALSEHE-EGCDESKDICKYRYSYGDNSFTKGDVATETISIDSSSGSSVSFPG 208

Query: 207 LTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGN----KFSYCIGDITDPFYPHNT 266
             FGCG+ N  T  +++ +GI+GLGG P +++ +QLG+    KFSYC+          + 
Sbjct: 209 TVFGCGYNNGGT-FEETGSGIIGLGGGP-LSLVSQLGSSIGKKFSYCLSHTAATTNGTSV 268

Query: 267 LILGNGAFTQGDSTPMHILF---------GHYYVTLQAISLGSNTLDIDPRVFRFTGHGS 326
           + LG  +     S     L           +Y++TL+A+++G   L      +   G  S
Sbjct: 269 INLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGKTKLPYTGGGYGLNGKSS 328

Query: 327 ---GGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLGRDLV 386
              G ++IDSG T T L    ++     + + + G  +R+   +     C++   G   +
Sbjct: 329 KRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTG-AKRVSDPQGLLTHCFKS--GDKEI 388

Query: 387 GFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNYNVGF 441
           G PA+T HF   AD+ L P + F +   D  CL+++P+     ++++ G + Q ++ VG+
Sbjct: 389 GLPAITMHFT-NADVKLSPINAFVKLNEDTVCLSMIPT----TEVAIYGNMVQMDFLVGY 441

BLAST of Sed0014583 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-48
Identity = 127/438 (29.00%), Postives = 214/438 (48.86%), Query Frame = 0

Query: 24  PSRLATKLIHRNSYLHPLYDPNETVEDRTKREQTSSFKRFTYLESKIKELNSIGDVAQSN 83
           P   + +LIHR+S L P+Y+P  TV DR       S  R      ++ + +      QS 
Sbjct: 23  PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTD-----LQSG 82

Query: 84  LIPFNQGSGFLVNLSIGLPPVAQLLAVDTGSYLLWVQCLPCVECFRQRNSWFDPSKSTSF 143
           LI       F ++++IG PP+      DTGS L WVQC PC +C+++    FD  KS+++
Sbjct: 83  LI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTY 142

Query: 144 EVLGCGFPGYNYISGYK--CNGFNE-AEYKLRYLGGDTSEGILAKESLTFDTSDEGRITK 203
           +   C       +S  +  C+  N   +Y+  Y     S+G +A E+++ D++    ++ 
Sbjct: 143 KSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSF 202

Query: 204 ANLTFGCGHMNKKTNRDDSYNGILGLGGYPHITMATQLGN----KFSYCIGDITDPFYPH 263
               FGCG+ N  T  D++ +GI+GLGG  H+++ +QLG+    KFSYC+   +      
Sbjct: 203 PGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLGSSISKKFSYCLSHKSATTNGT 262

Query: 264 NTLILGNGAFTQGDSTPMHIL---------FGHYYVTLQAISLGSNTL-----DIDPRVF 323
           + + LG  +     S    ++           +YY+TL+AIS+G   +       +P   
Sbjct: 263 SVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDD 322

Query: 324 RFTGHGSGGVLIDSGMTYTRLAKPGFEALCDGIVDLMMGLLERIPTRRDFEGLCYRGVLG 383
                 SG ++IDSG T T L    F+     + + + G  +R+   +     C++   G
Sbjct: 323 GILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTG-AKRVSDPQGLLSHCFKS--G 382

Query: 384 RDLVGFPAVTFHFDGGADLVLEPGSLFRQHGPDRFCLAVLPSDSEMLDLSVIGILAQQNY 441
              +G P +T HF  GAD+ L P + F +   D  CL+++P+     ++++ G  AQ ++
Sbjct: 383 SAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPT----TEVAIYGNFAQMDF 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031742409.11.6e-20176.70aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_... [more]
XP_022134792.11.6e-20177.65probable aspartic protease At2g35615 [Momordica charantia][more]
XP_023515827.12.1e-20176.32aspartic proteinase CDR1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023515828.16.1e-20177.59probable aspartic protease At2g35615 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_038878960.11.0e-20075.79aspartic proteinase CDR1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q3EBM51.6e-4729.00Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q6XBF81.0e-4432.48Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C23.8e-4430.37Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C31.3e-3932.30Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9SV772.4e-3831.58Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LUP57.8e-20276.70Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G42435... [more]
A0A6J1C0L17.8e-20277.65probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A5D3DZ208.7e-20176.92Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 ... [more]
A0A5A7UU118.7e-20176.92Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A6J1E1951.1e-20076.32probable aspartic protease At2g35615 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT4G30030.17.0e-8642.78Eukaryotic aspartyl protease family protein [more]
AT2G23945.17.0e-7840.09Eukaryotic aspartyl protease family protein [more]
AT4G30040.11.5e-7242.27Eukaryotic aspartyl protease family protein [more]
AT1G31450.16.8e-4930.18Eukaryotic aspartyl protease family protein [more]
AT2G35615.11.2e-4829.00Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 93..261
e-value: 6.0E-33
score: 114.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 266..442
e-value: 4.5E-39
score: 135.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 79..262
e-value: 8.1E-36
score: 125.8
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 90..441
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 280..435
e-value: 2.4E-27
score: 95.7
NoneNo IPR availablePANTHERPTHR47967:SF14EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 17..442
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 17..442
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 93..436
score: 31.451286
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 92..440
e-value: 1.57254E-71
score: 224.835

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0014583.1Sed0014583.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0016757 glycosyltransferase activity