Homology
BLAST of Sed0014431 vs. NCBI nr
Match:
XP_038891960.1 (alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1641.3 bits (4249), Expect = 0.0e+00
Identity = 779/904 (86.17%), Postives = 829/904 (91.70%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MSTI +P+LYHCGKGK RFH+ PRFNTL PCSFSYCPNKLL HGR F N NSYR P
Sbjct: 1 MSTISLDPLLYHCGKGKHRFHYRPRFNTLKPCSFSYCPNKLLHHGRKSFVNYNSYRLPTF 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
RA +TNA LL+ FQS DV +ETFPL R EKLEGRI VRLA+G +N+WELTVGC+L+GK
Sbjct: 61 RASATNAPLLQTFQSKDVLFQETFPLKRNEKLEGRISVRLAQGNDQNQWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSIPIKDYAIETP+KK SSSGD VHEVKI+L
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSSGDFVHEVKINL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
VPDKTIAAINFVLKDE+TGIWYQHKGRDFKVPLLD+HG+ GNIVGTKKGLGLWPGALGQL
Sbjct: 181 VPDKTIAAINFVLKDEQTGIWYQHKGRDFKVPLLDYHGEDGNIVGTKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K ES+SK + SGE D KEEKK L+GFYEELPIVKEVAVDNSVSVSVRKCPET
Sbjct: 241 SNLLVKAESSSKDKGRSGESGDAKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRKCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
K LLY+ETDLPGDVIVHWGACRDETRKWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KNLLYLETDLPGDVIVHWGACRDETRKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELED--TQGNF 420
CGIFT+EE+F GFLFVLKQNENSWLNYKGDDFYIP+S+SG+LP QQ++SEL+D
Sbjct: 361 CGIFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLSNSGNLPGQQRESELKDAGVSKKS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQESIL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKE+AAELS
Sbjct: 481 SSAPTFTEEVIEMPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKERAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGD VLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFP GKEMQGY YILTHPGTPS F+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPCGKEMQGYAYILTHPGTPSAFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVV IEKAERDVYAAIIDEKVAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVTIEKAERDVYAAIIDEKVAVKIGPGNFEPPSGSSGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. NCBI nr
Match:
KAA0034391.1 (alpha-amylase 3 [Cucumis melo var. makuwa])
HSP 1 Score: 1614.7 bits (4180), Expect = 0.0e+00
Identity = 765/904 (84.62%), Postives = 824/904 (91.15%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MS+I +P+LYHC K K RFHH PRFN L PCSF+YCPNKLL HGR F + NSYR P
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
+A +TNA FQS+DV KETFPL R EKLEGRI V+LA+GK N+WELTVGC+L+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSI IKDYAIETP+KK SSSGDVVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLD+ G+ GNIVG KKGLGLWPGALGQL
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K E+NSK Q SS E DTKE+KK L+GFYEELPIVKEVAVDNS+SVSV+KCPET
Sbjct: 241 SNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEVAVDNSISVSVKKCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+E+DLPGDV+VHWGACRD+TRKWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQGN--F 420
G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP+ SSG+LP QQ++S+L+D+ +
Sbjct: 361 SGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLPDQQRKSKLKDSGASKKS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQE+IL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQENILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGDAVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDAVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y+LTHPGTPSVF+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKI KAERDVYAA IDE VAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPSGSSGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. NCBI nr
Match:
XP_022151132.1 (alpha-amylase 3, chloroplastic [Momordica charantia])
HSP 1 Score: 1610.1 bits (4168), Expect = 0.0e+00
Identity = 772/904 (85.40%), Postives = 818/904 (90.49%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MSTIG +P+LYHCGKGK RFH P+FNTL PCSF Y PNKLL GR FFN NSYR P L
Sbjct: 1 MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
R STNA LLE FQS+DV LKETFPL RTEK+EG IFVRLA+GK +N+WELTVGC+L+GK
Sbjct: 61 RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSS---GDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSIPIKDYAIETP+KKSSS GD VHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
VP+K IAAINFVLKDEETGIWYQHKGRDFKVPL D HGD GNIVGTKKGLGLWPGALGQL
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K ES SKGQESSGE DTKE K L+GFYEELPIVKEVA DNSVSVSVR CPET
Sbjct: 241 SNLLVKAES-SKGQESSGESGDTKE--KYLEGFYEELPIVKEVAFDNSVSVSVRNCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+ETDLPGDVIVHWGA RDETRKWEIPAAPHP ET FKNKALRTLLQ KEGG+GS
Sbjct: 301 KYLLYLETDLPGDVIVHWGASRDETRKWEIPAAPHPPETTVFKNKALRTLLQLKEGGKGS 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELED--TQGNF 420
CGIFT+EEEF GFLFVLKQNEN WLNYKGDDFYIP SSG LP Q++Q+E ED
Sbjct: 361 CGIFTIEEEFDGFLFVLKQNENPWLNYKGDDFYIPFPSSGHLPGQKRQNEFEDAGVSRKA 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
G+G E VS T YTDGIIKEIRN VTDISSQ +KKK+ EAQESIL+EIEKLA+EAYSIFR
Sbjct: 421 GDGYEEVSVTAYTDGIIKEIRNFVTDISSQRTQKKKTIEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
S++PTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SASPTFTEEIIEMPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTV+WLPPPTESVSPEGYMPKDLYNL+SRYG+IDELKDVV+TFHDVGIKVLGD VLN
Sbjct: 541 SLGFTVIWLPPPTESVSPEGYMPKDLYNLSSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWL WLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLRWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
+QDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSD+KGKPPGVVGWWPS
Sbjct: 721 HDQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y LTHPGTPSVFFDHIFSHYKS+IA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYTLTHPGTPSVFFDHIFSHYKSDIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKIEKAERDVYAA IDEKVA+KIGPG YEPPSRS WSL IEG DYKVW
Sbjct: 841 RKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGYYEPPSRSNGWSLVIEGNDYKVW 900
BLAST of Sed0014431 vs. NCBI nr
Match:
XP_008446314.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo])
HSP 1 Score: 1610.1 bits (4168), Expect = 0.0e+00
Identity = 763/904 (84.40%), Postives = 821/904 (90.82%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MS+I +P+LYHC K K RFHH PRFN L PCSF+YCPNKLL HGR F + NSYR P
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
+A +TNA FQS+DV KETFPL R EKLEGRI V+LA+GK N+WELTVGC+L+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSI IKDYAIETP+KK SSSGDVVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLD+ G+ GNIVG KKGLGLWPGALGQL
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K E+NSK Q SS E DTKE+KK L+GFYEELPIVKEVAVDNS+SVSV+KCPET
Sbjct: 241 SNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEVAVDNSISVSVKKCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+E+DLPGDV+VHWGACRD+TRKWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQ--GNF 420
G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP+ SSG+LP QQ++S+ + +
Sbjct: 361 SGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLPDQQRKSKXKTAEHLKKS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQE+IL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQENILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGDAVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDAVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y+LTHPGTPSVF+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKI KAERDVYAA IDE VAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPSGSSGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. NCBI nr
Match:
XP_022967951.1 (alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 768/905 (84.86%), Postives = 821/905 (90.72%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPC-SFSYCPNKLLRHGRTCFFNRNSYRFPV 60
MSTI +P+LYHCGKGK RF P+FN L C SF+YC NKLL GR FFNRNS + PV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60
Query: 61 LRAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSG 120
LRA STNA LLEPF+S+DV L ETF + RTEKLEG+I V LA+GK +N+WELT+GC+LSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSS---GDVVHEVKID 180
KWILHWGVSLIDD+ SEWDQPPKEMIPPGSIPIKDYAIETP+KKSSS GD VHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQ 240
LVPDK IAAINFVLKDEETG WYQHKGRDFKVPLL +HG GNIVGTKKGL LWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPET 300
LSNL++KPE NSKGQESSGE DTK EK L+GFYEELPIVKEVAVDNSVSV+VRKCPET
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTK-EKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPET 300
Query: 301 AKYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEG 360
KY+LY+ETD+PGDV VHWGACRDETRKWEIPAAPHP ET FK KALRT LQPK+ G+G
Sbjct: 301 TKYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKG 360
Query: 361 SCGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQGNF- 420
SCGIFT+EEEF GFLFVLKQNENSWLN +G+DFYIP SSSG++PVQ+KQ +LEDT +
Sbjct: 361 SCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIK 420
Query: 421 -GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIF 480
GEGSEGVS T YT GIIKEIRNLVTDISSQ KKK+KEAQESIL+EIEKLA+EAYSIF
Sbjct: 421 SGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIF 480
Query: 481 RSSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAEL 540
RSS PTFTEE++ETP P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAEL
Sbjct: 481 RSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAEL 540
Query: 541 SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVL 600
SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGD VL
Sbjct: 541 SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVL 600
Query: 601 NHRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV 660
NHRCA F+NQNGIWN+FGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV
Sbjct: 601 NHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV 660
Query: 661 RNDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM 720
RNDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM
Sbjct: 661 RNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM 720
Query: 721 DRNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWP 780
D NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSD+KGKPPGVVGWWP
Sbjct: 721 DHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWP 780
Query: 781 SRAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLIT 840
SRAVTFIENHDTGSTQGHWRFPGGKEMQGY YILTHPGTPSVFFDHI SHYKSEIA+LIT
Sbjct: 781 SRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALIT 840
Query: 841 LRQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKV 900
LRQR KVNCRS VKI+KAERDVYAA ID+KVAVKIGPG++EPPS SG WSL IEG DYKV
Sbjct: 841 LRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKV 900
BLAST of Sed0014431 vs. ExPASy Swiss-Prot
Match:
Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)
HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 606/907 (66.81%), Postives = 719/907 (79.27%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFN----TLSPCSFS----YCPNKLLRH-GRTCFFN 60
MST+ E +L+H H + N + P S + + NKLL G++ +
Sbjct: 1 MSTVPIESLLHHS-----YLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60
Query: 61 RNSYRFPVLRAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWEL 120
+ +RA S++ ++E QS DV KE FP+ R EK EG+I+VRL + K +N WEL
Sbjct: 61 SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120
Query: 121 TVGCDLSGKWILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSSGDVVH 180
+VGC + GKWILHWGVS + DT SEWDQPP++M PPGSI IKDYAIETP+KK S GD
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGDSFF 180
Query: 181 EVKIDLVPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWP 240
EV I+L + ++AA+NFVLKDEETG WYQHKGRDFKVPL+D D GN++G KKG
Sbjct: 181 EVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF---- 240
Query: 241 GALGQLSNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVR 300
GALGQLSN+ +K Q+ S D+ EE+K LQ FYEE+PI K VA DNSVSV+ R
Sbjct: 241 GALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTAR 300
Query: 301 KCPETAKYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPK 360
KCPET+K ++ +ETDLPGDV VHWG C++ T+KWEIP+ P+P ET FKNKALRT LQ K
Sbjct: 301 KCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRK 360
Query: 361 EGGEGSCGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDT 420
+ G GS G+F+++ + G FVLK NEN+WLNY+G+DFY+P +S PV+ + +++
Sbjct: 361 DDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVETEAAQVSKP 420
Query: 421 QGNFGEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAY 480
+ + + VS + +T II EIRNL DISS +K KE QE+IL+EIEKLA+EAY
Sbjct: 421 K---RKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAY 480
Query: 481 SIFRSSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKA 540
SIFRS+ P F+EE + + +KISSGTG+GFEILCQGFNWES+K GRWY+EL+EKA
Sbjct: 481 SIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKA 540
Query: 541 AELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGD 600
EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKD V+ FH VGIKVLGD
Sbjct: 541 DELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGD 600
Query: 601 AVLNHRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQ 660
AVLNHRCAHFKNQNG+WNLFGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQ
Sbjct: 601 AVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQ 660
Query: 661 DFVRNDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTY 720
DFVR DIKEWLCW+ EE+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTY
Sbjct: 661 DFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTY 720
Query: 721 GEMDRNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVG 780
GEMD NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVG
Sbjct: 721 GEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVG 780
Query: 781 WWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIAS 840
WWPSRAVTFIENHDTGSTQGHWRFP GKEMQGY YILTHPGTP+VFFDHIFS Y SEIA+
Sbjct: 781 WWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAA 840
Query: 841 LITLRQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKD 899
L++LR RQK++CRS V I+K+ERDVYAAIIDEKVA+KIGPG+YEPP+ S WS+A+EG+D
Sbjct: 841 LLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRD 887
BLAST of Sed0014431 vs. ExPASy Swiss-Prot
Match:
Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)
HSP 1 Score: 426.0 bits (1094), Expect = 1.0e-117
Identity = 196/395 (49.62%), Postives = 268/395 (67.85%), Query Frame = 0
Query: 505 GFEILCQGFNWESHKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 564
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 565 NSRYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLFGG-RLNWDDRAIV 624
NS YGS LK ++R ++ + D V+NHR + G++N + G L WD+ A+
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 625 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDFVRGFW 684
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 685 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDRNQDAHRQRIVDWINATNGTAGAFDVTT 744
YVK+Y+ A++P F+VGE WDS +Y +D NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 745 KGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 804
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 805 YTYILTHPGTPSVFFDHIF---SHYKSEIASLITLRQRQKVNCRSVVKIEKAERDVYAAI 864
Y YILTHPG P VF+DH + S +I LI +R+RQ ++ RS V++ KAE ++YAAI
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 865 IDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 896
+ EK+ +K+G G++ P R W+LA G Y VW
Sbjct: 384 VGEKICMKLGDGSWCPSGRD--WTLATSGHRYAVW 411
BLAST of Sed0014431 vs. ExPASy Swiss-Prot
Match:
P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)
HSP 1 Score: 399.1 bits (1024), Expect = 1.4e-109
Identity = 201/402 (50.00%), Postives = 263/402 (65.42%), Query Frame = 0
Query: 508 ILCQGFNWESHKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 567
+L QGFNWES K G WY LK +L++ G T +WLPPP++SVSPEGY+P LY+L+ S
Sbjct: 25 LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84
Query: 568 RYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLFGG-----RLNWDDRA 627
+YGS +ELK ++ FH+ GIK L D V+NHR A K+ GI+ +F G R +W
Sbjct: 85 KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144
Query: 628 IVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDFVR 687
I DD + G GN SG+ + AAP+IDH V+ ++ EW+ WL+ EIG+DGWR DFV+
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204
Query: 688 GFWGGYVKDYLDASEPYFAVGEYWDSLSYTY-GEMDRNQDAHRQRIVDWINATNGTAGAF 747
G+ K Y++ ++P FAVGE WDS+SY G+ + NQD+HR +V+W+ + G AF
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAF 264
Query: 748 DVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGK 807
D TTKGIL +A++ E WRL D GKPPG++G P AVTFI+NHDTGSTQ W FP K
Sbjct: 265 DFTTKGILQAAVQG-ELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324
Query: 808 EMQGYTYILTHPGTPSVFFDHIFS-HYKSEIASLITLRQRQKVNCRSVVKIEKAERDVYA 867
MQGY YILTHPGTPS+F+DH F K +IA L ++R R +N +S VKI +E D+Y
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYV 384
Query: 868 AIIDEKVAVKIGP----GNYEPPSRSGRWSLAIEGKDYKVWE 897
A ID K+ VKIGP GN P +A G+DY VWE
Sbjct: 385 AKIDNKIMVKIGPKMDLGNLIP----SNLHVATSGQDYAVWE 421
BLAST of Sed0014431 vs. ExPASy Swiss-Prot
Match:
P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)
HSP 1 Score: 391.3 bits (1004), Expect = 2.9e-107
Identity = 191/400 (47.75%), Postives = 257/400 (64.25%), Query Frame = 0
Query: 507 EILCQGFNWES-HKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 566
++L QGFNWES K G WY L EK E++S G T +WLPPP+ SVSP+GYMP LY+L+
Sbjct: 26 QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85
Query: 567 -SRYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLF-----GGRLNWDD 626
S+YG+ ELK ++ FHD ++ L D V+NHRCA +K+ G++ +F GRL+W
Sbjct: 86 ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145
Query: 627 RAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDF 686
I SDD + GRG++ +G F AAP+IDH V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205
Query: 687 VRGFWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGEMDRNQDAHRQRIVDWINATNGTAG 746
+G+ + Y+D + P F VGE W SL Y G+ NQDA RQ +V+W+ A
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265
Query: 747 AFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 806
AFD TTKGIL +A++ E WRL D GK PG++GW P +AVTF++NHDTGSTQ W FP
Sbjct: 266 AFDFTTKGILQAAVQG-ELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPS 325
Query: 807 GKEMQGYTYILTHPGTPSVFFDHIFS-HYKSEIASLITLRQRQKVNCRSVVKIEKAERDV 866
K MQGY YILTHPG P +F+DH+F + + EIA+L +R R ++ S + I KAE D+
Sbjct: 326 DKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385
Query: 867 YAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVWE 897
Y A+ID KV K+GP + + G DY VWE
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424
BLAST of Sed0014431 vs. ExPASy Swiss-Prot
Match:
P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)
HSP 1 Score: 387.1 bits (993), Expect = 5.4e-106
Identity = 189/406 (46.55%), Postives = 260/406 (64.04%), Query Frame = 0
Query: 507 EILCQGFNWES-HKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 566
+IL QGFNW+S K G WY LK++ +++S G T +WLPPPT SVSP+GYMP LY+LN
Sbjct: 29 QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88
Query: 567 -SRYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLFGGR-----LNWDD 626
S+YG+ ELK ++ FH GIK + D V+NHRCA K+ G++ +F G L+W
Sbjct: 89 ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148
Query: 627 RAIVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDF 686
I DD + G G++ +G +F AAP+IDH V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208
Query: 687 VRGFWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGEMDRNQDAHRQRIVDWINATNGTAG 746
+G+ + Y+ + P F V E W+SLSY G+ NQD RQ +V+W+ G A
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268
Query: 747 AFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 806
AFD TTKGIL SA++ E WR+ D+ GK PG++GW+P +AVTF++NHDTGSTQ W FP
Sbjct: 269 AFDFTTKGILQSAVQG-ELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPS 328
Query: 807 GKEMQGYTYILTHPGTPSVFFDHIFS-HYKSEIASLITLRQRQKVNCRSVVKIEKAERDV 866
K + GY YILTHPG P +F+DH+F + K EI +L R+R +N S +++ AE D+
Sbjct: 329 DKVILGYAYILTHPGVPCIFYDHVFDWNLKQEINALAATRKRNGINAGSKLRVLAAESDM 388
Query: 867 YAAIIDEKVAVKIGP----GNYEPPSRSGRWSLAIEGKDYKVWEVS 899
Y A++DE+V KIGP GN P + + G DY VWE S
Sbjct: 389 YVAMVDERVITKIGPRIDVGNIIPSD----FHIVAHGNDYCVWEKS 429
BLAST of Sed0014431 vs. ExPASy TrEMBL
Match:
A0A5A7SY64 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003970 PE=4 SV=1)
HSP 1 Score: 1614.7 bits (4180), Expect = 0.0e+00
Identity = 765/904 (84.62%), Postives = 824/904 (91.15%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MS+I +P+LYHC K K RFHH PRFN L PCSF+YCPNKLL HGR F + NSYR P
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
+A +TNA FQS+DV KETFPL R EKLEGRI V+LA+GK N+WELTVGC+L+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSI IKDYAIETP+KK SSSGDVVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLD+ G+ GNIVG KKGLGLWPGALGQL
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K E+NSK Q SS E DTKE+KK L+GFYEELPIVKEVAVDNS+SVSV+KCPET
Sbjct: 241 SNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEVAVDNSISVSVKKCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+E+DLPGDV+VHWGACRD+TRKWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQGN--F 420
G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP+ SSG+LP QQ++S+L+D+ +
Sbjct: 361 SGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLPDQQRKSKLKDSGASKKS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQE+IL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQENILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGDAVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDAVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y+LTHPGTPSVF+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKI KAERDVYAA IDE VAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPSGSSGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. ExPASy TrEMBL
Match:
A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)
HSP 1 Score: 1610.1 bits (4168), Expect = 0.0e+00
Identity = 772/904 (85.40%), Postives = 818/904 (90.49%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MSTIG +P+LYHCGKGK RFH P+FNTL PCSF Y PNKLL GR FFN NSYR P L
Sbjct: 1 MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
R STNA LLE FQS+DV LKETFPL RTEK+EG IFVRLA+GK +N+WELTVGC+L+GK
Sbjct: 61 RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSS---GDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSIPIKDYAIETP+KKSSS GD VHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
VP+K IAAINFVLKDEETGIWYQHKGRDFKVPL D HGD GNIVGTKKGLGLWPGALGQL
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K ES SKGQESSGE DTKE K L+GFYEELPIVKEVA DNSVSVSVR CPET
Sbjct: 241 SNLLVKAES-SKGQESSGESGDTKE--KYLEGFYEELPIVKEVAFDNSVSVSVRNCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+ETDLPGDVIVHWGA RDETRKWEIPAAPHP ET FKNKALRTLLQ KEGG+GS
Sbjct: 301 KYLLYLETDLPGDVIVHWGASRDETRKWEIPAAPHPPETTVFKNKALRTLLQLKEGGKGS 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELED--TQGNF 420
CGIFT+EEEF GFLFVLKQNEN WLNYKGDDFYIP SSG LP Q++Q+E ED
Sbjct: 361 CGIFTIEEEFDGFLFVLKQNENPWLNYKGDDFYIPFPSSGHLPGQKRQNEFEDAGVSRKA 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
G+G E VS T YTDGIIKEIRN VTDISSQ +KKK+ EAQESIL+EIEKLA+EAYSIFR
Sbjct: 421 GDGYEEVSVTAYTDGIIKEIRNFVTDISSQRTQKKKTIEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
S++PTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SASPTFTEEIIEMPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTV+WLPPPTESVSPEGYMPKDLYNL+SRYG+IDELKDVV+TFHDVGIKVLGD VLN
Sbjct: 541 SLGFTVIWLPPPTESVSPEGYMPKDLYNLSSRYGNIDELKDVVKTFHDVGIKVLGDVVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWL WLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLRWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
+QDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSD+KGKPPGVVGWWPS
Sbjct: 721 HDQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y LTHPGTPSVFFDHIFSHYKS+IA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYTLTHPGTPSVFFDHIFSHYKSDIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKIEKAERDVYAA IDEKVA+KIGPG YEPPSRS WSL IEG DYKVW
Sbjct: 841 RKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGYYEPPSRSNGWSLVIEGNDYKVW 900
BLAST of Sed0014431 vs. ExPASy TrEMBL
Match:
A0A1S3BES1 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4 SV=1)
HSP 1 Score: 1610.1 bits (4168), Expect = 0.0e+00
Identity = 763/904 (84.40%), Postives = 821/904 (90.82%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MS+I +P+LYHC K K RFHH PRFN L PCSF+YCPNKLL HGR F + NSYR P
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
+A +TNA FQS+DV KETFPL R EKLEGRI V+LA+GK N+WELTVGC+L+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSI IKDYAIETP+KK SSSGDVVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLD+ G+ GNIVG KKGLGLWPGALGQL
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K E+NSK Q SS E DTKE+KK L+GFYEELPIVKEVAVDNS+SVSV+KCPET
Sbjct: 241 SNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEVAVDNSISVSVKKCPETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+E+DLPGDV+VHWGACRD+TRKWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQ--GNF 420
G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP+ SSG+LP QQ++S+ + +
Sbjct: 361 SGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLPDQQRKSKXKTAEHLKKS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQE+IL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQENILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IE P P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGDAVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDAVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y+LTHPGTPSVF+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKI KAERDVYAA IDE VAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPSGSSGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. ExPASy TrEMBL
Match:
A0A6J1HTI2 (1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 PE=3 SV=1)
HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 768/905 (84.86%), Postives = 821/905 (90.72%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPC-SFSYCPNKLLRHGRTCFFNRNSYRFPV 60
MSTI +P+LYHCGKGK RF P+FN L C SF+YC NKLL GR FFNRNS + PV
Sbjct: 1 MSTIVLDPLLYHCGKGKQRFQRRPKFNPLKSCSSFTYCTNKLLYRGRRTFFNRNSCKLPV 60
Query: 61 LRAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSG 120
LRA STNA LLEPF+S+DV L ETF + RTEKLEG+I V LA+GK +N+WELT+GC+LSG
Sbjct: 61 LRASSTNAGLLEPFESTDVLLTETFFIKRTEKLEGKISVGLAQGKNQNQWELTIGCNLSG 120
Query: 121 KWILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSS---GDVVHEVKID 180
KWILHWGVSLIDD+ SEWDQPPKEMIPPGSIPIKDYAIETP+KKSSS GD VHEVKID
Sbjct: 121 KWILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSALGDAVHEVKID 180
Query: 181 LVPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQ 240
LVPDK IAAINFVLKDEETG WYQHKGRDFKVPLL +HG GNIVGTKKGL LWPGALGQ
Sbjct: 181 LVPDKAIAAINFVLKDEETGTWYQHKGRDFKVPLLGYHGGDGNIVGTKKGLNLWPGALGQ 240
Query: 241 LSNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPET 300
LSNL++KPE NSKGQESSGE DTK EK L+GFYEELPIVKEVAVDNSVSV+VRKCPET
Sbjct: 241 LSNLIVKPEPNSKGQESSGESGDTK-EKNYLEGFYEELPIVKEVAVDNSVSVTVRKCPET 300
Query: 301 AKYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEG 360
KY+LY+ETD+PGDV VHWGACRDETRKWEIPAAPHP ET FK KALRT LQPK+ G+G
Sbjct: 301 TKYVLYLETDVPGDVTVHWGACRDETRKWEIPAAPHPPETTVFKKKALRTPLQPKKDGKG 360
Query: 361 SCGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQGNF- 420
SCGIFT+EEEF GFLFVLKQNENSWLN +G+DFYIP SSSG++PVQ+KQ +LEDT +
Sbjct: 361 SCGIFTVEEEFGGFLFVLKQNENSWLNNQGEDFYIPFSSSGNMPVQKKQIKLEDTGVSIK 420
Query: 421 -GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIF 480
GEGSEGVS T YT GIIKEIRNLVTDISSQ KKK+KEAQESIL+EIEKLA+EAYSIF
Sbjct: 421 SGEGSEGVSVTAYTVGIIKEIRNLVTDISSQKTPKKKTKEAQESILQEIEKLAAEAYSIF 480
Query: 481 RSSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAEL 540
RSS PTFTEE++ETP P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAEL
Sbjct: 481 RSSTPTFTEEILETPKPVEPPVQISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAEL 540
Query: 541 SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVL 600
SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGD VL
Sbjct: 541 SSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDVVL 600
Query: 601 NHRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV 660
NHRCA F+NQNGIWN+FGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV
Sbjct: 601 NHRCAQFQNQNGIWNIFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFV 660
Query: 661 RNDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM 720
RNDIKEWLCWLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM
Sbjct: 661 RNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEM 720
Query: 721 DRNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWP 780
D NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSD+KGKPPGVVGWWP
Sbjct: 721 DHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALQRCEYWRLSDQKGKPPGVVGWWP 780
Query: 781 SRAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLIT 840
SRAVTFIENHDTGSTQGHWRFPGGKEMQGY YILTHPGTPSVFFDHI SHYKSEIA+LIT
Sbjct: 781 SRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHILSHYKSEIAALIT 840
Query: 841 LRQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKV 900
LRQR KVNCRS VKI+KAERDVYAA ID+KVAVKIGPG++EPPS SG WSL IEG DYKV
Sbjct: 841 LRQRNKVNCRSAVKIQKAERDVYAATIDDKVAVKIGPGHFEPPSISGGWSLVIEGNDYKV 900
BLAST of Sed0014431 vs. ExPASy TrEMBL
Match:
A0A0A0KQJ3 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 PE=4 SV=1)
HSP 1 Score: 1602.4 bits (4148), Expect = 0.0e+00
Identity = 765/904 (84.62%), Postives = 821/904 (90.82%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFNTLSPCSFSYCPNKLLRHGRTCFFNRNSYRFPVL 60
MS+I +P+LYHC KGK RFHH PRFN L PCSF+YCPNKLL HGR F + NSYR P +
Sbjct: 1 MSSIALDPLLYHCAKGKHRFHHRPRFNMLRPCSFTYCPNKLLCHGRKSFVHYNSYRPPTI 60
Query: 61 RAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWELTVGCDLSGK 120
+A +TNA FQS+DV ETFPL R EKLEGRI VRLA+GK N WELTVGC+L+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFNETFPLKRNEKLEGRISVRLAQGKDHNNWELTVGCNLAGK 120
Query: 121 WILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKK---SSSGDVVHEVKIDL 180
WILHWGVSLIDD+ SEWDQPPKEMIPPGSI IKDYAIETP+KK SSSGD VHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGD-VHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWPGALGQL 240
PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLD+ G+ GN VGTKKGLGLWPGALGQL
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNKVGTKKGLGLWPGALGQL 240
Query: 241 SNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVRKCPETA 300
SNLL+K E+NSK Q SS E DTKEEKK L+GFY+ELPIVKE+AVDNS+SVSVRKC ET
Sbjct: 241 SNLLVKAETNSKDQGSSSESGDTKEEKKSLEGFYKELPIVKEIAVDNSISVSVRKCSETT 300
Query: 301 KYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPKEGGEGS 360
KYLLY+E+DLPGDVIVHWGACRD+T+KWEIPAAPHP ET FKNKALRTLLQPKEGG+G
Sbjct: 301 KYLLYLESDLPGDVIVHWGACRDDTKKWEIPAAPHPPETTVFKNKALRTLLQPKEGGKGC 360
Query: 361 CGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDTQGN--F 420
G+FT+EE+F GFLFVLKQ ENSWLNYKGDDFYIP SSG+L QQ++S+L+DT+ +
Sbjct: 361 SGVFTIEEDFGGFLFVLKQKENSWLNYKGDDFYIPFPSSGNLSNQQRKSKLKDTRASKIS 420
Query: 421 GEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAYSIFR 480
GE SEGVS T YTDGIIKEIRNLVTDISSQ +KKK+KEAQESIL+EIEKLA+EAYSIFR
Sbjct: 421 GEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQESILQEIEKLAAEAYSIFR 480
Query: 481 SSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKAAELS 540
SSAPTFTEE+IETP P E V+ISSGTG+GFEILCQGFNWESHK GRWYMELKEKAAELS
Sbjct: 481 SSAPTFTEEIIETPKPVEPPVRISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELS 540
Query: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGDAVLN 600
SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKDVV+TFHDVGIKVLGDAVLN
Sbjct: 541 SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVVKTFHDVGIKVLGDAVLN 600
Query: 601 HRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
HRCAHFKNQNGIWN+FGGRLNWDDRA+VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR
Sbjct: 601 HRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVR 660
Query: 661 NDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
NDIKEWL WLR+EIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD
Sbjct: 661 NDIKEWLLWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMD 720
Query: 721 RNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPS 780
NQDAHRQRIVDWINATNGTAGAFDVTTKGILHSAL+RCEYWRLSDEKGKPPGVVGWWPS
Sbjct: 721 HNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPS 780
Query: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIASLITL 840
RAVTFIENHDTGSTQGHWRFPGGKEMQGY Y+LTHPGTPSVF+DHIFSHYKSEIA+LI+L
Sbjct: 781 RAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFYDHIFSHYKSEIAALISL 840
Query: 841 RQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 900
R+R KVNCRSVVKI KAERDVYAAIIDE VAVKIGPGN+EPPS S WSL IEGKDYKVW
Sbjct: 841 RKRNKVNCRSVVKIVKAERDVYAAIIDETVAVKIGPGNFEPPSGSNGWSLVIEGKDYKVW 900
BLAST of Sed0014431 vs. TAIR 10
Match:
AT1G69830.1 (alpha-amylase-like 3 )
HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 606/907 (66.81%), Postives = 719/907 (79.27%), Query Frame = 0
Query: 1 MSTIGFEPVLYHCGKGKLRFHHLPRFN----TLSPCSFS----YCPNKLLRH-GRTCFFN 60
MST+ E +L+H H + N + P S + + NKLL G++ +
Sbjct: 1 MSTVPIESLLHHS-----YLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60
Query: 61 RNSYRFPVLRAGSTNAVLLEPFQSSDVFLKETFPLIRTEKLEGRIFVRLAKGKGRNEWEL 120
+ +RA S++ ++E QS DV KE FP+ R EK EG+I+VRL + K +N WEL
Sbjct: 61 SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120
Query: 121 TVGCDLSGKWILHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSSGDVVH 180
+VGC + GKWILHWGVS + DT SEWDQPP++M PPGSI IKDYAIETP+KK S GD
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGDSFF 180
Query: 181 EVKIDLVPDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDHHGDGGNIVGTKKGLGLWP 240
EV I+L + ++AA+NFVLKDEETG WYQHKGRDFKVPL+D D GN++G KKG
Sbjct: 181 EVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF---- 240
Query: 241 GALGQLSNLLIKPESNSKGQESSGEPDDTKEEKKDLQGFYEELPIVKEVAVDNSVSVSVR 300
GALGQLSN+ +K Q+ S D+ EE+K LQ FYEE+PI K VA DNSVSV+ R
Sbjct: 241 GALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISKRVADDNSVSVTAR 300
Query: 301 KCPETAKYLLYMETDLPGDVIVHWGACRDETRKWEIPAAPHPRETKAFKNKALRTLLQPK 360
KCPET+K ++ +ETDLPGDV VHWG C++ T+KWEIP+ P+P ET FKNKALRT LQ K
Sbjct: 301 KCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRK 360
Query: 361 EGGEGSCGIFTMEEEFRGFLFVLKQNENSWLNYKGDDFYIPVSSSGDLPVQQKQSELEDT 420
+ G GS G+F+++ + G FVLK NEN+WLNY+G+DFY+P +S PV+ + +++
Sbjct: 361 DDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSSSPVETEAAQVSKP 420
Query: 421 QGNFGEGSEGVSTTTYTDGIIKEIRNLVTDISSQNARKKKSKEAQESILEEIEKLASEAY 480
+ + + VS + +T II EIRNL DISS +K KE QE+IL+EIEKLA+EAY
Sbjct: 421 K---RKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAY 480
Query: 481 SIFRSSAPTFTEELIETPTPAESAVKISSGTGTGFEILCQGFNWESHKCGRWYMELKEKA 540
SIFRS+ P F+EE + + +KISSGTG+GFEILCQGFNWES+K GRWY+EL+EKA
Sbjct: 481 SIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKA 540
Query: 541 AELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKDVVRTFHDVGIKVLGD 600
EL+SLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYG+IDELKD V+ FH VGIKVLGD
Sbjct: 541 DELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGD 600
Query: 601 AVLNHRCAHFKNQNGIWNLFGGRLNWDDRAIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQ 660
AVLNHRCAHFKNQNG+WNLFGGRLNWDDRA+V+DDPHFQGRGNKSSGDNFHAAPNIDHSQ
Sbjct: 601 AVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQ 660
Query: 661 DFVRNDIKEWLCWLREEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTY 720
DFVR DIKEWLCW+ EE+GYDGWRLDFVRGFWGGYVKDY+DAS+PYFAVGEYWDSLSYTY
Sbjct: 661 DFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTY 720
Query: 721 GEMDRNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDEKGKPPGVVG 780
GEMD NQDAHRQRIVDWINAT+G AGAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVG
Sbjct: 721 GEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVG 780
Query: 781 WWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYTYILTHPGTPSVFFDHIFSHYKSEIAS 840
WWPSRAVTFIENHDTGSTQGHWRFP GKEMQGY YILTHPGTP+VFFDHIFS Y SEIA+
Sbjct: 781 WWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAA 840
Query: 841 LITLRQRQKVNCRSVVKIEKAERDVYAAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKD 899
L++LR RQK++CRS V I+K+ERDVYAAIIDEKVA+KIGPG+YEPP+ S WS+A+EG+D
Sbjct: 841 LLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRD 887
BLAST of Sed0014431 vs. TAIR 10
Match:
AT1G76130.1 (alpha-amylase-like 2 )
HSP 1 Score: 426.0 bits (1094), Expect = 7.4e-119
Identity = 196/395 (49.62%), Postives = 268/395 (67.85%), Query Frame = 0
Query: 505 GFEILCQGFNWESHKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 564
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 565 NSRYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLFGG-RLNWDDRAIV 624
NS YGS LK ++R ++ + D V+NHR + G++N + G L WD+ A+
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 625 SDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDFVRGFW 684
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 685 GGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDRNQDAHRQRIVDWINATNGTAGAFDVTT 744
YVK+Y+ A++P F+VGE WDS +Y +D NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 745 KGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 804
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 805 YTYILTHPGTPSVFFDHIF---SHYKSEIASLITLRQRQKVNCRSVVKIEKAERDVYAAI 864
Y YILTHPG P VF+DH + S +I LI +R+RQ ++ RS V++ KAE ++YAAI
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 865 IDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVW 896
+ EK+ +K+G G++ P R W+LA G Y VW
Sbjct: 384 VGEKICMKLGDGSWCPSGRD--WTLATSGHRYAVW 411
BLAST of Sed0014431 vs. TAIR 10
Match:
AT4G25000.1 (alpha-amylase-like )
HSP 1 Score: 347.8 bits (891), Expect = 2.6e-95
Identity = 179/399 (44.86%), Postives = 243/399 (60.90%), Query Frame = 0
Query: 508 ILCQGFNWES-HKCGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN- 567
+L Q FNWES K G +Y L ++++ G T LWLPPP++SV+PEGY+P LY+LN
Sbjct: 27 LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86
Query: 568 SRYGSIDELKDVVRTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNLFGG-----RLNWDDR 627
S+YGS ELK +++ + GIK L D V+NHR A K+ + F G RL+WD
Sbjct: 87 SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146
Query: 628 AIVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLREEIGYDGWRLDFVR 687
+ +DP F G GN +G +F AP+IDH V+ ++ EW+ WL+ EIG+ GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206
Query: 688 GFWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGEMDRNQDAHRQRIVDWI-NATNGTAGA 747
G+ K Y+ + P FAVGE WD + Y G++D +Q+ HR + WI A G A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266
Query: 748 FDVTTKGILHSALERCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGG 807
FD TTKGIL SA+ + E WRL D +GKPPG++G P AVTFI+NHDT T W FP
Sbjct: 267 FDFTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSD 326
Query: 808 KEMQGYTYILTHPGTPSVFFDHIFS-HYKSEIASLITLRQRQKVNCRSVVKIEKAERDVY 867
K + GY YILTHPGTP +F++H K I+ L+ +R + + S V I+ AE D+Y
Sbjct: 327 KVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLY 386
Query: 868 AAIIDEKVAVKIGPGNYEPPSRSGRWSLAIEGKDYKVWE 897
A+ID+KV +KIGP ++LA G D+ VWE
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421
BLAST of Sed0014431 vs. TAIR 10
Match:
AT1G10760.1 (Pyruvate phosphate dikinase, PEP/pyruvate binding domain )
HSP 1 Score: 46.2 bits (108), Expect = 1.6e-04
Identity = 28/88 (31.82%), Postives = 43/88 (48.86%), Query Frame = 0
Query: 123 LHWGVSLIDDTASEWDQPPKEMIPPGSIPIKDYAIETPMKKSSSGDVVHEVKIDLVPDKT 182
LHWG L D W P + P + K+ A+ TP KS +K++ + D
Sbjct: 120 LHWGAIL--DNKENWVLPSRS--PDRTQNFKNSALRTPFVKSGGNS---HLKLE-IDDPA 179
Query: 183 IAAINFVLKDEETGIWYQHKGRDFKVPL 211
I AI F++ DE WY++ G++F + L
Sbjct: 180 IHAIEFLIFDESRNKWYKNNGQNFHINL 199
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038891960.1 | 0.0e+00 | 86.17 | alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida] | [more] |
KAA0034391.1 | 0.0e+00 | 84.62 | alpha-amylase 3 [Cucumis melo var. makuwa] | [more] |
XP_022151132.1 | 0.0e+00 | 85.40 | alpha-amylase 3, chloroplastic [Momordica charantia] | [more] |
XP_008446314.1 | 0.0e+00 | 84.40 | PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo] | [more] |
XP_022967951.1 | 0.0e+00 | 84.86 | alpha-amylase 3, chloroplastic-like isoform X1 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q94A41 | 0.0e+00 | 66.81 | Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1 | [more] |
Q8LFG1 | 1.0e-117 | 49.62 | Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1 | [more] |
P17859 | 1.4e-109 | 50.00 | Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1 | [more] |
P27934 | 2.9e-107 | 47.75 | Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... | [more] |
P27932 | 5.4e-106 | 46.55 | Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SY64 | 0.0e+00 | 84.62 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... | [more] |
A0A6J1DCP4 | 0.0e+00 | 85.40 | 1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110191... | [more] |
A0A1S3BES1 | 0.0e+00 | 84.40 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4... | [more] |
A0A6J1HTI2 | 0.0e+00 | 84.86 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467335 ... | [more] |
A0A0A0KQJ3 | 0.0e+00 | 84.62 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 P... | [more] |