Sed0014413 (gene) Chayote v1

Overview
NameSed0014413
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
LocationLG10: 32116086 .. 32121604 (+)
RNA-Seq ExpressionSed0014413
SyntenySed0014413
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAATCGAGTTATGAAACAATGGCCATTTTGCCAATTCCCTAATTTTGGGTCGACGATGGAGGGTCTTAAACTCAACAATGGACGATGAATAAAGCTCATCGACTAACTTCTTCACTCCCAATCCACAGTCTTTTTCTCTCTGAAATGGGTTTTCACGTTCTTAGAGCCCCGCCTTTGTGTTCTTCCACAACCATAATCTTCAAACCCACTTCTTCCGCTTCTCTTCTCTTCAAATTTGGCTATGCTGCTTTCTCCAAATCGTCCCCTTCCTCCACCAGACCCTATTTCCGAGCTTCTTCTCCACCCAAATACCTTCCTTCAATTCCGCTTTATGGGTCTTTTTCTTCCAGAGCTCGTTGTTTTCGTGGTGGGTTTGTTGCAGCCATGGCTGCAGCACCTGGGTCTGTCCACAAATCCGAGGAATTGAGGCCTTCTATCTCGCCCGAGCAATCTTGGGTTCAGAGGCAAAATGGGATTGAGTAAGATTTTATATTGTTTTACTGTGTATTTTGTAATTTGGAGGCCTAGAGGTTTTTGGGTCAATAGTATTATGTTGTTAGAGGATGAAATTTGGAGCTCGACGAGCTACTTGAAAGTACATATATACGCCAGCCGCCAGCAGTGTTTTAAAAAGCTCAAGGCGCACTAAGGGGCATTGGCCCTCATGAGGTGCAAGACGCACCAAAGGGTGCACCAAAGGGTGCACCAAAAGGCGCGGGCTTTTTACTAAAGGCGCATACATGTATACATACACATCTCTTTCAATATAGAGATTTTTACATAGTCATCCCATAGGGTCGACGATTAGGTTTTAATTGTTTTTTTAAAAATAAAACTTTAAAGTAATTGTCGATTGAATTTGAAATTGTAAAAACGAATAGATAAATATGAGTAAAACATTACCGTTTGACTTAGATTTTAAATAAATAAAAAAATTCCTCAAGACGTGCGACTTGCACGCATGAGGGCTTTATGAAAAGGCACGTGCCTGCACAGGCGAGGCGCCAACATTGAGCCTGAGGCGCGCCTTTTCGGGCTTTTTAAAACACTACGGCCAGCTTACCCTCACCTTGACTATTGTCATGACATTTGGATGTCTAAGAAACCAGCAGAAAATTAATTATTAGGCAGGTGGCTAGTGTAGATTGAATTCATTTACCTCTTAGTTACTTTAAGGCTCTTGAACATTAGGGCACTTCATGATGGTTGCTACTTATAATTGAAGTGACTGATCCTTGTTTCGAAGTCTATGCTTTTTTAACTTTTAAGGGTGTTACTTTATAAATTGGTTTGAATTTTGTGTACAAATTTAGACTCCTTAAGGCACTAATTTCTGATATATTGTTGGTTTTGATTGAGAGTCTGAGACTTGAAGTTCTGTTACCTTGTTAGTGTACCCATGTTTTCTCCTGATAGCTGGTAGTTCAGGGTTATGTCTAATTTAATTTGTGGATGCAGATAGTAAACTGAGCAAAACAATAGATCTAACAACCTTGCTTAGAAACATAGATTTCTTCGTTTGGTTGATAAGGTGAAATTACCAAAGAGGTCAAATAATGCCAAATGACACCGGCCTTTCAAAGCATCAAGGATTGAGTTAATGATGCTGATTGCTTTGTAAAATCCTCTTATGTTTAATCTCAAGAAAAAGTAAAGACTTCTCATTGGTGAAGATGTAGGAATAGTGGGGAAACGTTTTGGCTATTTTTTCCTCTCTTCTCATTTCTTATGCTCTAGCAAATTATATATTTTGAGCGATTTAAAATCTCTTGGTGTGCTGCTCATAAGGCTTCAATGACTGTAGATTCTGTTTGACCACGTTTTTGTAGGCAAAGGGTGGACCCTTTGGGAGTAATTGTACAAGGGTTTATGTAACACAATTTTGTTGCAAATGAATGAGTTTCTTTTTGTATTCGTGAGTGTCCGAGCCAGTTTACGTGCACCTTGACTATTCTCATGGAACATACTGGCTGATGCTTCAACATTTGGATATCAAGGAAACCCGTAGGAAATTAATTCCTAAGTAGGTGGCCACCATGGGTTGAACCCATCACCCCTTAGTTATCTAACGCCCTTTGTGACCAGTTGACCACTTCATGATGGTTAAATGAATGAATTAATAACTGAAGGAGTTTAAAGCTTTTATTTATTTTGGAGGTCTTGGAGCATATGCATTGCAGCCTTAAAATTGGGGCTCTCTAGCTAGGGACTTTTGTATCTACTATCCCTTTATAACTATTGACATGCCGCTTTTGAGAGGGAGGAGAAAGATAATATGTTTGATAAATGATAATATGTTTGATAACTATTGTCATGTCCAAACTCTGAACGTTACTTTTATTCTTATCCTTGACCAGATCCGTACCAGATTCTGAAGTGCCTATAAGAGGTCGTATTTATCATGAAACTTATGGGTGTCAAATGAATATTAATGATATGGAGGTTGTTCTATCAATCATGAAAAATGCTGGATATAGTGAAACCGTCGACATTCCCGAGACTGCAGAGATCATTTTTATCAACACTTGTGCAATCAGGGACAATGCCGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGACAGTGGAAAAGTAATGTTGCTACTGGAAGGTCACAGTCTCAGCACCCTCCAAAAGTAGTTGTATTGGGGTGCATGGCTGAGAGGTTGAAAGATAAGATACTAGATGCAGACAAGATGGTGGATGTGGTTTGTGGACCTGATGCTTACAGAGACCTACCACGATTATTGGATGAAGTTGATTATGGTCAAAAGGGAATAAACACACTTCTTTCCCTCGAAGAAACTTATGCTGACATAAGTCCAGTACGAATATCTAAAAACTCAATCACTGCATTTGTATCCGTGATGAGAGGTTGCAACAATATGTGTTCGTTCTGCATTGTTCCTTTCACTCGAGGCAGAGAGCGGTCACGTCCTGTCGAATCAATTGTGGATGAAGTTAGAGAACTTTATAGACAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAATGTTAACAGCTATAATGATATCGCTGAGAACACAGATGTGGAACCGGGGACTAATTGGAAACTCAGTGATGGATTTTCCACCATAGCCAAGGTAAAGAAAATGGGTTTGCGTTTTTCTGATCTATTAGATCGTCTTTCCACAGAATTTCCTGAGATGAGGTTTCGATACACGTCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACCGACACAATATTTGCAAAAGTATCCATTTACCTGCTCAGAGTGGGAATAGCGCAGTTCTTGAAAGAATGCGTAGGGGATATACTCGGGAGGCCTACTTGGATCTTGTGCGTAAGGTTCGATCAATCATTCCGGATGTGGGAATAACTAGTGATTTCATTTGTGGTAAGTGGCCTTTTTAACTATAGTATTTCGATTAGATGTCGGTTATTATATTTATGCATATGATACGTTGTTGTACCGTTAAAGTAGTTGGTCTTAAAAAGATAGTTTCTTAGAAATGCCTAGGCAATGGCAGTGGTGATCGGAATGGTGGATTTTTCTTTTTGAAATCGGAAGATTGGAGCTTGCTCCACTATACCCAGGGACTGGGTAACCATGCCCATCCTGTCCCTAGATCAGGCACCGAGAGACATCGAAGTAATGGTTGGAATGATGGATTTAAAACTGTATTTTTTTAAAAATAATTCTTTTCATCCCTATCCATTCTATCTATGATAAAATGTTAGCATTGGGAATCAGATTGCAAATTGTGTGCGAGAAGTCTAAACTTCATCTAAAATACTAAGGGGAGTGTTCGGGCCCCGCTTATGTAAACGGCCGGGTTGGTTATAATAACCCAACCAATGTTTGCCCCACCGTTTATTATAGACGGTAGTTACCTAAACCTGCGTGTCCGTTTATCTAAACCTAAGTGCCCGTTTATCTAAACCTCACACATATTACTTTTTGTTACATAACCAGGCTCTCAAACACCATTTATCATAATTCCTATTATAATAATTTACCCCTCAAACACCATTTATAATAACCCACTGTTTATCATAACCTTCCGTCTATTATAACAAAACCGGTGCTCCAAACATACCCTAGGAGATTCGTTAGTGTCAGTTAACCAGCTAATGCTTTTTCTTTCATTCAATTTCTCTCCTCCTTTCTATTTCTATGTCATGAATTAGATTCAACTTCGTTTTAGTGCCTATACTGTTCACTTTTTATTTTGATCTTTTGTAACTTCTAAAATGTAATTGTCCCCAAGAGGTGATGGGGTGGCCGAAAGTTTTGGTTTGAAGATGTACTCTCCTCCAGGTCTCAAGTTCAAGACTCACCTGTGATATTAATTTGTAAACACCTCTCGTATCTCCATTATTTTGATGTCTCCCAGTGCTTGGCCTAGAGACGGGCGTGGTTACCCCAGACATAGTGGAGTGAAGCTCCGATTTCTCAGTTTCTTTCCGAAAAAAGAACTAAAATGTAATCCAAATTTATGAAGTTTTGTTACATTATTCTTGTTAAAGAAGAAAAAAATACTCTGTAGTGCTGATATTGCTATTTAGGCTTAAACAAGAAAAGCTTCCGTGGCATTTGGTCTCAAACCTGTAACCTTACTATAGGTCATACGTGATGTCACTGCCCTTTTGGCATTGGTTTAGTAAATAATGCTTCACTTTCTTTCATTTTGGTCTGGCAAAAAGTTATCATGGTTGGGTGCTAGTATAGAATTTTGAGCATCATTCTTTTTCTGCTGAAAAACATTCTTAAACTCTTTGTATAGGTTTCTGCGGTGAGACAGAAGAGGAACATGCCGACACTCTAAGTTTGGTTAAGGAAGTCGGGTATGACATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATATTGACGACGTTCCAGAGGAAGTGAAGCAGAGAAGACTTACCGAACTCATCGAAACTTTTCGAAACAGTACAGGACGATGCTACGATTCGGAAATTGGCAGTGTTCAATTGGTGCTTGTGGAAGGACCAAACAAAAGAGCCCCAGAGACGGAGCTGATTGGCAAGAGTGACAGAGGCCATCGAGTTTCGTTTGTCAATGTAGCAGTACCACATCGAGATGGTGATTACGATGGACAACGGAAAGCAGCGATTGGCGACTTCGTGGAAGTCCATATATCGAAGTCGACACGAGCATCATTGTTCGGAGAGGCACTTGCCATTACAAAACTAAGCTCATTCTACAACGGTATGGATGAATCAATTGCCTGCGTGAGCTGAAGTTCGAATGAATTTAGATCAGTTTGAATTTTGATAAAATGTTGTTGCTTTAGTCTTCAACTACCTCTTTAATGCATGGGGTGCTTTTATTTATTTATTTATTTGTTATCATTGCACCTTTTTTAATGTTCGAAGTGTAAAAATATCATTGTATCTTGGTTATAGTTGTCAAATGTTCTCTCAAACACATGATTTACAAAGATTGTGCCTG

mRNA sequence

AAATAATCGAGTTATGAAACAATGGCCATTTTGCCAATTCCCTAATTTTGGGTCGACGATGGAGGGTCTTAAACTCAACAATGGACGATGAATAAAGCTCATCGACTAACTTCTTCACTCCCAATCCACAGTCTTTTTCTCTCTGAAATGGGTTTTCACGTTCTTAGAGCCCCGCCTTTGTGTTCTTCCACAACCATAATCTTCAAACCCACTTCTTCCGCTTCTCTTCTCTTCAAATTTGGCTATGCTGCTTTCTCCAAATCGTCCCCTTCCTCCACCAGACCCTATTTCCGAGCTTCTTCTCCACCCAAATACCTTCCTTCAATTCCGCTTTATGGGTCTTTTTCTTCCAGAGCTCGTTGTTTTCGTGGTGGGTTTGTTGCAGCCATGGCTGCAGCACCTGGGTCTGTCCACAAATCCGAGGAATTGAGGCCTTCTATCTCGCCCGAGCAATCTTGGGTTCAGAGGCAAAATGGGATTGAATCCGTACCAGATTCTGAAGTGCCTATAAGAGGTCGTATTTATCATGAAACTTATGGGTGTCAAATGAATATTAATGATATGGAGGTTGTTCTATCAATCATGAAAAATGCTGGATATAGTGAAACCGTCGACATTCCCGAGACTGCAGAGATCATTTTTATCAACACTTGTGCAATCAGGGACAATGCCGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGACAGTGGAAAAGTAATGTTGCTACTGGAAGGTCACAGTCTCAGCACCCTCCAAAAGTAGTTGTATTGGGGTGCATGGCTGAGAGGTTGAAAGATAAGATACTAGATGCAGACAAGATGGTGGATGTGGTTTGTGGACCTGATGCTTACAGAGACCTACCACGATTATTGGATGAAGTTGATTATGGTCAAAAGGGAATAAACACACTTCTTTCCCTCGAAGAAACTTATGCTGACATAAGTCCAGTACGAATATCTAAAAACTCAATCACTGCATTTGTATCCGTGATGAGAGGTTGCAACAATATGTGTTCGTTCTGCATTGTTCCTTTCACTCGAGGCAGAGAGCGGTCACGTCCTGTCGAATCAATTGTGGATGAAGTTAGAGAACTTTATAGACAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAATGTTAACAGCTATAATGATATCGCTGAGAACACAGATGTGGAACCGGGGACTAATTGGAAACTCAGTGATGGATTTTCCACCATAGCCAAGGTAAAGAAAATGGGTTTGCGTTTTTCTGATCTATTAGATCGTCTTTCCACAGAATTTCCTGAGATGAGGTTTCGATACACGTCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACCGACACAATATTTGCAAAAGTATCCATTTACCTGCTCAGAGTGGGAATAGCGCAGTTCTTGAAAGAATGCGTAGGGGATATACTCGGGAGGCCTACTTGGATCTTGTGCGTAAGGTTCGATCAATCATTCCGGATGTGGGAATAACTAGTGATTTCATTTGTGGTTTCTGCGGTGAGACAGAAGAGGAACATGCCGACACTCTAAGTTTGGTTAAGGAAGTCGGGTATGACATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATATTGACGACGTTCCAGAGGAAGTGAAGCAGAGAAGACTTACCGAACTCATCGAAACTTTTCGAAACAGTACAGGACGATGCTACGATTCGGAAATTGGCAGTGTTCAATTGGTGCTTGTGGAAGGACCAAACAAAAGAGCCCCAGAGACGGAGCTGATTGGCAAGAGTGACAGAGGCCATCGAGTTTCGTTTGTCAATGTAGCAGTACCACATCGAGATGGTGATTACGATGGACAACGGAAAGCAGCGATTGGCGACTTCGTGGAAGTCCATATATCGAAGTCGACACGAGCATCATTGTTCGGAGAGGCACTTGCCATTACAAAACTAAGCTCATTCTACAACGGTATGGATGAATCAATTGCCTGCGTGAGCTGAAGTTCGAATGAATTTAGATCAGTTTGAATTTTGATAAAATGTTGTTGCTTTAGTCTTCAACTACCTCTTTAATGCATGGGGTGCTTTTATTTATTTATTTATTTGTTATCATTGCACCTTTTTTAATGTTCGAAGTGTAAAAATATCATTGTATCTTGGTTATAGTTGTCAAATGTTCTCTCAAACACATGATTTACAAAGATTGTGCCTG

Coding sequence (CDS)

ATGAATAAAGCTCATCGACTAACTTCTTCACTCCCAATCCACAGTCTTTTTCTCTCTGAAATGGGTTTTCACGTTCTTAGAGCCCCGCCTTTGTGTTCTTCCACAACCATAATCTTCAAACCCACTTCTTCCGCTTCTCTTCTCTTCAAATTTGGCTATGCTGCTTTCTCCAAATCGTCCCCTTCCTCCACCAGACCCTATTTCCGAGCTTCTTCTCCACCCAAATACCTTCCTTCAATTCCGCTTTATGGGTCTTTTTCTTCCAGAGCTCGTTGTTTTCGTGGTGGGTTTGTTGCAGCCATGGCTGCAGCACCTGGGTCTGTCCACAAATCCGAGGAATTGAGGCCTTCTATCTCGCCCGAGCAATCTTGGGTTCAGAGGCAAAATGGGATTGAATCCGTACCAGATTCTGAAGTGCCTATAAGAGGTCGTATTTATCATGAAACTTATGGGTGTCAAATGAATATTAATGATATGGAGGTTGTTCTATCAATCATGAAAAATGCTGGATATAGTGAAACCGTCGACATTCCCGAGACTGCAGAGATCATTTTTATCAACACTTGTGCAATCAGGGACAATGCCGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGACAGTGGAAAAGTAATGTTGCTACTGGAAGGTCACAGTCTCAGCACCCTCCAAAAGTAGTTGTATTGGGGTGCATGGCTGAGAGGTTGAAAGATAAGATACTAGATGCAGACAAGATGGTGGATGTGGTTTGTGGACCTGATGCTTACAGAGACCTACCACGATTATTGGATGAAGTTGATTATGGTCAAAAGGGAATAAACACACTTCTTTCCCTCGAAGAAACTTATGCTGACATAAGTCCAGTACGAATATCTAAAAACTCAATCACTGCATTTGTATCCGTGATGAGAGGTTGCAACAATATGTGTTCGTTCTGCATTGTTCCTTTCACTCGAGGCAGAGAGCGGTCACGTCCTGTCGAATCAATTGTGGATGAAGTTAGAGAACTTTATAGACAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAATGTTAACAGCTATAATGATATCGCTGAGAACACAGATGTGGAACCGGGGACTAATTGGAAACTCAGTGATGGATTTTCCACCATAGCCAAGGTAAAGAAAATGGGTTTGCGTTTTTCTGATCTATTAGATCGTCTTTCCACAGAATTTCCTGAGATGAGGTTTCGATACACGTCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACCGACACAATATTTGCAAAAGTATCCATTTACCTGCTCAGAGTGGGAATAGCGCAGTTCTTGAAAGAATGCGTAGGGGATATACTCGGGAGGCCTACTTGGATCTTGTGCGTAAGGTTCGATCAATCATTCCGGATGTGGGAATAACTAGTGATTTCATTTGTGGTTTCTGCGGTGAGACAGAAGAGGAACATGCCGACACTCTAAGTTTGGTTAAGGAAGTCGGGTATGACATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATATTGACGACGTTCCAGAGGAAGTGAAGCAGAGAAGACTTACCGAACTCATCGAAACTTTTCGAAACAGTACAGGACGATGCTACGATTCGGAAATTGGCAGTGTTCAATTGGTGCTTGTGGAAGGACCAAACAAAAGAGCCCCAGAGACGGAGCTGATTGGCAAGAGTGACAGAGGCCATCGAGTTTCGTTTGTCAATGTAGCAGTACCACATCGAGATGGTGATTACGATGGACAACGGAAAGCAGCGATTGGCGACTTCGTGGAAGTCCATATATCGAAGTCGACACGAGCATCATTGTTCGGAGAGGCACTTGCCATTACAAAACTAAGCTCATTCTACAACGGTATGGATGAATCAATTGCCTGCGTGAGCTGA

Protein sequence

MNKAHRLTSSLPIHSLFLSEMGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRASSPPKYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESVPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVEVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS
Homology
BLAST of Sed0014413 vs. NCBI nr
Match: XP_038887766.1 (CDK5RAP1-like protein isoform X2 [Benincasa hispida])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 577/639 (90.30%), Postives = 599/639 (93.74%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSK-----SSPSSTRPYFRASSPPK 80
           MGF+VLRAPP  SSTTII KPTSSASLLFKFGYA +S      SS SS+R YFRASS  +
Sbjct: 1   MGFYVLRAPPFSSSTTIILKPTSSASLLFKFGYALYSSPFSKFSSSSSSRTYFRASS-AR 60

Query: 81  YLPS----IPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGI 140
           Y+ S    IPL GS   R R FRGGF+AA  AAPGSV+KSEEL+PSISPEQ WVQR+NG 
Sbjct: 61  YVASTATAIPLSGSLPRRTRSFRGGFIAA-TAAPGSVYKSEELKPSISPEQPWVQRKNGT 120

Query: 141 ESVPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAI 200
           ES+P SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAI
Sbjct: 121 ESLPASEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAI 180

Query: 201 RDNAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMV 260
           RDNAEQKVWQRLNYFWFLKRQWKSNVA GRS+SQHPPKVVVLGCMAERLKDKILDADKMV
Sbjct: 181 RDNAEQKVWQRLNYFWFLKRQWKSNVAIGRSKSQHPPKVVVLGCMAERLKDKILDADKMV 240

Query: 261 DVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCN 320
           DVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCN
Sbjct: 241 DVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCN 300

Query: 321 NMCSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPG 380
           NMCSFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYND AEN DVEPG
Sbjct: 301 NMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTAENADVEPG 360

Query: 381 TNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRH 440
           TNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRH
Sbjct: 361 TNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRH 420

Query: 441 NICKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEE 500
           NICKSIHLPAQSGNS VLERMRRGYTREAYLDLV K+RSIIPDVGITSDFICGFCGETEE
Sbjct: 421 NICKSIHLPAQSGNSTVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEE 480

Query: 501 EHADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGR 560
           EHADTLSL+ EVGYDMAY+FAYSMREKTHAHRNY+DDVPEEVKQRRLTELIETFRNSTGR
Sbjct: 481 EHADTLSLISEVGYDMAYLFAYSMREKTHAHRNYVDDVPEEVKQRRLTELIETFRNSTGR 540

Query: 561 CYDSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGD 620
           CYDS+IGSVQLVLVEGPNKRAPETE+IGKSDRGHRVSF+NVAVPHRDGDYDGQRKA +GD
Sbjct: 541 CYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRGHRVSFINVAVPHRDGDYDGQRKAVVGD 600

Query: 621 FVEVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           FVEV ISKSTRASLFGEALAITKLSSFYN MDESIACVS
Sbjct: 601 FVEVRISKSTRASLFGEALAITKLSSFYNSMDESIACVS 637

BLAST of Sed0014413 vs. NCBI nr
Match: XP_022989605.1 (CDK5RAP1-like protein [Cucurbita maxima])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 570/636 (89.62%), Postives = 594/636 (93.40%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRAS------SPP 80
           MG HVLRAPP  SST+IIFKPTSSASLLFKFGYA    +SPSSTR YFR S      SPP
Sbjct: 1   MGLHVLRAPPFSSSTSIIFKPTSSASLLFKFGYAF---NSPSSTRTYFRVSSLKYVASPP 60

Query: 81  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESV 140
               SIPL G FS R R F GGF+AA  AAPGSV KSEELRPS+SP+QSWVQRQNGIE +
Sbjct: 61  ---TSIPLSGYFSRRTRRFHGGFIAA-TAAPGSVKKSEELRPSVSPDQSWVQRQNGIEPL 120

Query: 141 PDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDN 200
             SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVD+PETAE+IFINTCAIRDN
Sbjct: 121 TGSEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDVPETAEVIFINTCAIRDN 180

Query: 201 AEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVV 260
           AEQKVWQRLNYFWFLKR+WKSNVA GRSQS+HPPKVVVLGCMAERLKDKILDADKMVDVV
Sbjct: 181 AEQKVWQRLNYFWFLKREWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDADKMVDVV 240

Query: 261 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMC 320
           CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCNNMC
Sbjct: 241 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNMC 300

Query: 321 SFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNW 380
           SFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYNDI +NTDVEPGTNW
Sbjct: 301 SFCIVPFTRGRERSRPVESIVSEVRELYREGVKEVTLLGQNVNSYNDIIDNTDVEPGTNW 360

Query: 381 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 440
           KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRD+HNIC
Sbjct: 361 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDKHNIC 420

Query: 441 KSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHA 500
           KSIHLPAQSGNS VLERMRRGYTRE YLDLV K+RSIIPDVGITSDFICGFCGETEEEHA
Sbjct: 421 KSIHLPAQSGNSTVLERMRRGYTREVYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 480

Query: 501 DTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYD 560
           DTLSL+KEVGYDMAYMFAYSMREKTHAHRNY+DDVPEEVKQRRLTELIETFRNSTG+CYD
Sbjct: 481 DTLSLIKEVGYDMAYMFAYSMREKTHAHRNYVDDVPEEVKQRRLTELIETFRNSTGQCYD 540

Query: 561 SEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVE 620
            +IGS+QLVLVEGPNKRA ETE+IGKSDRGHRVSFVNVA+PHRDGDYDGQRK AIGDFVE
Sbjct: 541 MQIGSIQLVLVEGPNKRALETEMIGKSDRGHRVSFVNVAIPHRDGDYDGQRKVAIGDFVE 600

Query: 621 VHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           V ISKSTRASLFGEALA+TKLSSFYN MDESIACVS
Sbjct: 601 VRISKSTRASLFGEALAVTKLSSFYNSMDESIACVS 629

BLAST of Sed0014413 vs. NCBI nr
Match: XP_038887765.1 (CDK5RAP1-like protein isoform X1 [Benincasa hispida])

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 577/656 (87.96%), Postives = 599/656 (91.31%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSK-----SSPSSTRPYFRASSPPK 80
           MGF+VLRAPP  SSTTII KPTSSASLLFKFGYA +S      SS SS+R YFRASS  +
Sbjct: 1   MGFYVLRAPPFSSSTTIILKPTSSASLLFKFGYALYSSPFSKFSSSSSSRTYFRASS-AR 60

Query: 81  YLPS----IPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGI 140
           Y+ S    IPL GS   R R FRGGF+AA  AAPGSV+KSEEL+PSISPEQ WVQR+NG 
Sbjct: 61  YVASTATAIPLSGSLPRRTRSFRGGFIAA-TAAPGSVYKSEELKPSISPEQPWVQRKNGT 120

Query: 141 E-----------------SVPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSET 200
           E                 S+P SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSET
Sbjct: 121 ESSACLVEIGSQLRLIGRSLPASEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSET 180

Query: 201 VDIPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLG 260
           VDIPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKSNVA GRS+SQHPPKVVVLG
Sbjct: 181 VDIPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKSNVAIGRSKSQHPPKVVVLG 240

Query: 261 CMAERLKDKILDADKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVR 320
           CMAERLKDKILDADKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVR
Sbjct: 241 CMAERLKDKILDADKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVR 300

Query: 321 ISKNSITAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQ 380
           ISKNS+TAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQ
Sbjct: 301 ISKNSVTAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQ 360

Query: 381 NVNSYNDIAENTDVEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSP 440
           NVNSYND AEN DVEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSP
Sbjct: 361 NVNSYNDTAENADVEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSP 420

Query: 441 HPKDFPDEFLYLMRDRHNICKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPD 500
           HPKDFPDEFLYLMRDRHNICKSIHLPAQSGNS VLERMRRGYTREAYLDLV K+RSIIPD
Sbjct: 421 HPKDFPDEFLYLMRDRHNICKSIHLPAQSGNSTVLERMRRGYTREAYLDLVHKIRSIIPD 480

Query: 501 VGITSDFICGFCGETEEEHADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVK 560
           VGITSDFICGFCGETEEEHADTLSL+ EVGYDMAY+FAYSMREKTHAHRNY+DDVPEEVK
Sbjct: 481 VGITSDFICGFCGETEEEHADTLSLISEVGYDMAYLFAYSMREKTHAHRNYVDDVPEEVK 540

Query: 561 QRRLTELIETFRNSTGRCYDSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAV 620
           QRRLTELIETFRNSTGRCYDS+IGSVQLVLVEGPNKRAPETE+IGKSDRGHRVSF+NVAV
Sbjct: 541 QRRLTELIETFRNSTGRCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRGHRVSFINVAV 600

Query: 621 PHRDGDYDGQRKAAIGDFVEVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           PHRDGDYDGQRKA +GDFVEV ISKSTRASLFGEALAITKLSSFYN MDESIACVS
Sbjct: 601 PHRDGDYDGQRKAVVGDFVEVRISKSTRASLFGEALAITKLSSFYNSMDESIACVS 654

BLAST of Sed0014413 vs. NCBI nr
Match: XP_022927671.1 (CDK5RAP1-like protein [Cucurbita moschata])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 565/636 (88.84%), Postives = 589/636 (92.61%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRAS------SPP 80
           MG HVL +PP  SST+IIFKPTSSASLLFKFGYA     SPSST  Y R S      SPP
Sbjct: 1   MGLHVLISPPFSSSTSIIFKPTSSASLLFKFGYAL--SKSPSSTTTYLRPSSLKYVASPP 60

Query: 81  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESV 140
               SIPL G FS R R F GGF+AA  AAPGSV KSEELRPS+SP+QSWVQRQNGIE +
Sbjct: 61  ---TSIPLSGYFSRRTRRFHGGFIAA-TAAPGSVQKSEELRPSVSPDQSWVQRQNGIEPL 120

Query: 141 PDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDN 200
             SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVD+PETAE+IFINTCAIRDN
Sbjct: 121 TGSEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDVPETAEVIFINTCAIRDN 180

Query: 201 AEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVV 260
           AEQKVWQRLNYFWFLKR+WKSNVA GRSQS+HPPKVVVLGCMAERLKDKILDADKMVDVV
Sbjct: 181 AEQKVWQRLNYFWFLKREWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDADKMVDVV 240

Query: 261 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMC 320
           CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCNNMC
Sbjct: 241 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNMC 300

Query: 321 SFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNW 380
           SFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYND  +NTDVEPGTNW
Sbjct: 301 SFCIVPFTRGRERSRPVESIVSEVRELYREGVKEVTLLGQNVNSYNDTIDNTDVEPGTNW 360

Query: 381 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 440
           KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRD+HNIC
Sbjct: 361 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDKHNIC 420

Query: 441 KSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHA 500
           KSIHLPAQSGNS VLERMRRGYTRE YLDLV K+RSIIPDVGITSDFICGFCGETEEEHA
Sbjct: 421 KSIHLPAQSGNSTVLERMRRGYTREVYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 480

Query: 501 DTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYD 560
           DTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DDVPE+VKQRRLTELIETFRNSTG+CYD
Sbjct: 481 DTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDVPEDVKQRRLTELIETFRNSTGQCYD 540

Query: 561 SEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVE 620
            +IGS+QLVLVEGPNKRAPETE+IGKSDRGHRVSFVNVAVPHRDGDYDGQRK AIGDFVE
Sbjct: 541 MQIGSIQLVLVEGPNKRAPETEMIGKSDRGHRVSFVNVAVPHRDGDYDGQRKVAIGDFVE 600

Query: 621 VHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           V ISKSTRASLFGEALA+TKLSSFYN MDESIACVS
Sbjct: 601 VRISKSTRASLFGEALAVTKLSSFYNSMDESIACVS 630

BLAST of Sed0014413 vs. NCBI nr
Match: XP_023529365.1 (CDK5RAP1-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 563/636 (88.52%), Postives = 587/636 (92.30%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRAS------SPP 80
           MG HVL APP  SST+IIFKPTSSASLLFKFGYA     SPSST  Y R+S      SPP
Sbjct: 1   MGLHVLIAPPFSSSTSIIFKPTSSASLLFKFGYAL--SKSPSSTTTYLRSSSLKYVASPP 60

Query: 81  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESV 140
               S+PL G FS R R F GGF+AA  AAPGSV KSEELRPS+SP+QSWVQRQNGIE +
Sbjct: 61  ---TSLPLSGYFSRRTRRFHGGFIAA-TAAPGSVQKSEELRPSVSPDQSWVQRQNGIEPL 120

Query: 141 PDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDN 200
             SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVD+PETAE+IFINTCAIRDN
Sbjct: 121 TGSEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDVPETAEVIFINTCAIRDN 180

Query: 201 AEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVV 260
           AEQKVWQRLNYFWFLKR+WK NVA GRSQS+HPPKVVVLGCMAERLKDKILDADKMVDVV
Sbjct: 181 AEQKVWQRLNYFWFLKREWKGNVAIGRSQSRHPPKVVVLGCMAERLKDKILDADKMVDVV 240

Query: 261 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMC 320
           CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCNNMC
Sbjct: 241 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNMC 300

Query: 321 SFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNW 380
           SFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYND  +NTDVEPGTNW
Sbjct: 301 SFCIVPFTRGRERSRPVESIVSEVRELYREGVKEVTLLGQNVNSYNDTIDNTDVEPGTNW 360

Query: 381 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 440
           KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRD+HNIC
Sbjct: 361 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDKHNIC 420

Query: 441 KSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHA 500
           KSIHLPAQSGNS VLERMRRGYTRE YLDLV K+RSIIPDVGITSDFICGFCGETEEEHA
Sbjct: 421 KSIHLPAQSGNSTVLERMRRGYTREVYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 480

Query: 501 DTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYD 560
           DTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DDVPEEVKQRRLTELIETFRNSTG+CYD
Sbjct: 481 DTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDVPEEVKQRRLTELIETFRNSTGQCYD 540

Query: 561 SEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVE 620
            +IGS+QLVLVEGPNKRAPETE+IGKSDRGHRVSFVNVAVPHRDGDYDG RK A GDFVE
Sbjct: 541 MQIGSIQLVLVEGPNKRAPETEMIGKSDRGHRVSFVNVAVPHRDGDYDGHRKVAFGDFVE 600

Query: 621 VHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           V ISKSTRASLFGEALA+TKLSSFYN MDESIACVS
Sbjct: 601 VRISKSTRASLFGEALAVTKLSSFYNSMDESIACVS 630

BLAST of Sed0014413 vs. ExPASy Swiss-Prot
Match: Q8H0V1 (CDK5RAP1-like protein OS=Arabidopsis thaliana OX=3702 GN=At4g36390 PE=2 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 9.2e-241
Identity = 433/625 (69.28%), Postives = 495/625 (79.20%), Query Frame = 0

Query: 25  VLRAPPLCSSTTIIFKPTSSASLLFKF-GYAAFSKSSPSSTRPYFRASSPPKYLPSIPL- 84
           +L  P  CS   + FK ++      +F    A   SS SS+    R  S    L   P+ 
Sbjct: 12  ILSNPHGCS---LCFKASTQRCFALRFLSSKATHASSSSSSALLPRCRSSTHRLTQKPIN 71

Query: 85  --YGSFSSRARCFRGGFVAAMAAAPG-SVHKSEELRPSISPEQSWVQRQNGIESVPDSEV 144
              G   + +R F    +A+     G S+H   +   +     S    +   ES  DS++
Sbjct: 72  RKKGFSLNLSRSFSISQIASSGKFDGPSLH---QFVSNAQAHASLTTPETESESTLDSDI 131

Query: 145 PIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKV 204
             +GRIYHETYGCQMNINDME+VL+IMKN+GY E V  PE+AE+IF+NTCAIR+NAEQ+V
Sbjct: 132 ASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNTCAIRENAEQRV 191

Query: 205 WQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDA 264
           WQRLNYFWFLKR+WK N ATGR++S  PPKVVVLGCMAERLKDKILD+DKMVDVVCGPDA
Sbjct: 192 WQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDA 251

Query: 265 YRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIV 324
           YRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRIS+NSITAFVSVMRGCNNMC+FCIV
Sbjct: 252 YRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAFCIV 311

Query: 325 PFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDG 384
           PFTRGRERSRPVESI+ EV EL+  GVKEVTLLGQNVNSYND  ++ D E G NW+ S+G
Sbjct: 312 PFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYND--DSADRESGANWEYSEG 371

Query: 385 FSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHL 444
           FS+  KVK MGLRF+DLLDRLS EFPEMRFR+TSPHPKD+PDE LYLMRDRHNIC  IHL
Sbjct: 372 FSSRCKVKNMGLRFADLLDRLSVEFPEMRFRFTSPHPKDYPDELLYLMRDRHNICNLIHL 431

Query: 445 PAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSL 504
           PAQSGNS +LE+MRRGYTREAYLDLV+K+RSIIPDV ITSDFI GFCGETEEEH +TLSL
Sbjct: 432 PAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITSDFITGFCGETEEEHQETLSL 491

Query: 505 VKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGS 564
           V+ VGYDMAYMFAYSMREKTHAHRNY DDVPEEVKQRRLTELI+ FR +TG CYDS++GS
Sbjct: 492 VRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLTELIDAFRETTGPCYDSQVGS 551

Query: 565 VQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHR----DGDYDGQRKAAIGDFVEV 624
            QLVLVEGPNKRAPETELIGK+D+GHRVSFV   +  +    DGD D +R   IGDFVEV
Sbjct: 552 TQLVLVEGPNKRAPETELIGKTDKGHRVSFVTKPLFDKACLLDGD-DLKRNPGIGDFVEV 611

Query: 625 HISKSTRASLFGEALAITKLSSFYN 641
            I KSTRASLFGEALAI+K+S F++
Sbjct: 612 QIEKSTRASLFGEALAISKMSLFHD 627

BLAST of Sed0014413 vs. ExPASy Swiss-Prot
Match: Q2R1U4 (CDK5RAP1-like protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0592800 PE=2 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 1.3e-239
Identity = 410/566 (72.44%), Postives = 474/566 (83.75%), Query Frame = 0

Query: 75  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRP-SISPEQSWVQRQNGIES 134
           +Y P+ PL  S  +  RC            P  + +S   RP + S   +  + Q  +ES
Sbjct: 28  RYSPA-PLAPSPCAAPRCLSSA-AHPPPPPPRRLARSGPSRPLAASAATAVSEAQTDLES 87

Query: 135 VPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRD 194
            P      +GRIYHETYGCQMN+NDME+VLSIMKN GY E V  PE+AEIIFINTCAIRD
Sbjct: 88  GP--VTASKGRIYHETYGCQMNVNDMEIVLSIMKNEGYDEIVPDPESAEIIFINTCAIRD 147

Query: 195 NAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDV 254
           NAEQKVWQRLNYFWFLKRQWK+NVA GRS+S  PPK+ VLGCMAERLK+KILD+DKMVDV
Sbjct: 148 NAEQKVWQRLNYFWFLKRQWKANVAAGRSRSLRPPKIAVLGCMAERLKEKILDSDKMVDV 207

Query: 255 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNM 314
           VCGPDAYRDLPRLL EVDYGQKGINTLLSLEETYADI+PVRIS NS+TAFVS+MRGCNNM
Sbjct: 208 VCGPDAYRDLPRLLQEVDYGQKGINTLLSLEETYADITPVRISDNSVTAFVSIMRGCNNM 267

Query: 315 CSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTN 374
           CSFCIVPFTRGRERSRPV SIV EV EL++ GVKEV LLGQNVNSYND +E  ++EPG N
Sbjct: 268 CSFCIVPFTRGRERSRPVSSIVREVGELWKAGVKEVMLLGQNVNSYNDTSEVEELEPGKN 327

Query: 375 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 434
           W+LS+GFS++ KVK MGLRF+DLLD+LS E+PEMRFR+TSPHPKD+PDE LYLMRDRHN+
Sbjct: 328 WELSEGFSSMCKVKNMGLRFADLLDQLSLEYPEMRFRFTSPHPKDYPDELLYLMRDRHNV 387

Query: 435 CKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEH 494
           CK IH+PAQSG+SAVLERMRRGYTREAYL+LV+K+RSIIPDVG++SDFI GFCGETEEEH
Sbjct: 388 CKLIHMPAQSGSSAVLERMRRGYTREAYLELVQKIRSIIPDVGLSSDFISGFCGETEEEH 447

Query: 495 ADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCY 554
           A+TL+LV+ VGYDMAYMFAYSMREKTHAHRNY+DDVP++VKQRRL ELI TFR +T + Y
Sbjct: 448 AETLTLVRAVGYDMAYMFAYSMREKTHAHRNYVDDVPDDVKQRRLAELISTFRETTAKIY 507

Query: 555 DSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFV 614
           DS++G+VQLVLVEGPNKRAPETE+IGK+DRGHRVSF +V VPH   + D  RK  +GDF+
Sbjct: 508 DSQVGTVQLVLVEGPNKRAPETEMIGKTDRGHRVSFASVPVPH-TFEGDELRKPVVGDFI 567

Query: 615 EVHISKSTRASLFGEALAITKLSSFY 640
           EV I+KS+ ASL G+ +A T LS FY
Sbjct: 568 EVKITKSSTASLSGDVIARTSLSKFY 588

BLAST of Sed0014413 vs. ExPASy Swiss-Prot
Match: Q9JLH6 (Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Rattus norvegicus OX=10116 GN=Cdk5rap1 PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.5e-145
Identity = 273/507 (53.85%), Postives = 359/507 (70.81%), Query Frame = 0

Query: 144 RIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKVWQRL 203
           ++Y ETYGCQMN+ND E+  SI++ +GY  T ++ E A++I + TC+IR+ AEQ +W RL
Sbjct: 98  KVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQE-ADVILLVTCSIREKAEQTIWNRL 157

Query: 204 NYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDAYRDL 263
           +    LK +        R +S+ P ++ +LGCMAERLK +IL+ +KMVD++ GPDAYRDL
Sbjct: 158 HQLKVLKAK--------RPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL 217

Query: 264 PRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIVPFTR 323
           PRLL  V+ GQ+  N LLSL+ETYADI PV+ S ++ +AFVS+MRGC+NMCS+CIVPFTR
Sbjct: 218 PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTR 277

Query: 324 GRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDGFSTI 383
           GRERSRPV SI+DEVR+L  QG+KEVTLLGQNVNS+ D +E      G+   LS GF+T 
Sbjct: 278 GRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFSSTGSA-NLSRGFTTN 337

Query: 384 AKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQS 443
            K K+ GLRFS LLD++S   PEMR R+TSPHPKDFPDE L L+R+RHNICK IHLPAQS
Sbjct: 338 YKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQS 397

Query: 444 GNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSLVKEV 503
           G+S VLE MRRGY+REAY+ LV  +R  IP VG++SDFI GFCGETE++H  T+SL++EV
Sbjct: 398 GSSRVLEAMRRGYSREAYVALVHHIREAIPGVGLSSDFITGFCGETEDDHLQTVSLLREV 457

Query: 504 GYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGSVQLV 563
            Y+  ++FAYSMR+KT A+    DDVPEEVK RRL ELI  FR    +   + +G  QLV
Sbjct: 458 QYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKVNATSVGCTQLV 517

Query: 564 LVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVEVHISKSTRA 623
           LVEG +KR+  T+L G++D   +V F +  V     D   + +A  GD+V V I  ++  
Sbjct: 518 LVEGFSKRS-TTDLCGRNDANLKVIFPDAEVEDIT-DPGLKVRAQPGDYVLVKIISASSQ 577

Query: 624 SLFGEALAITKLSSFYNGMDESIACVS 651
           +L G  L  T +       D S+ C++
Sbjct: 578 TLKGHILCRTTMK------DSSMNCLT 586

BLAST of Sed0014413 vs. ExPASy Swiss-Prot
Match: Q8BTW8 (Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Mus musculus OX=10090 GN=Cdk5rap1 PE=1 SV=2)

HSP 1 Score: 516.2 bits (1328), Expect = 5.6e-145
Identity = 271/493 (54.97%), Postives = 354/493 (71.81%), Query Frame = 0

Query: 144 RIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKVWQRL 203
           ++Y ETYGCQMN+ND E+  SI++ +GY  T ++ E A++I + TC+IR+ AEQ +W RL
Sbjct: 100 KVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQE-ADVILLVTCSIREKAEQTIWNRL 159

Query: 204 NYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDAYRDL 263
           +    LK        T R +S+ P ++ +LGCMAERLK +IL+ +KMVD++ GPDAYRDL
Sbjct: 160 HQLKVLK--------TKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL 219

Query: 264 PRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIVPFTR 323
           PRLL  V+ GQ+  N LLSL+ETYADI PV+ S ++ +AFVS+MRGC+NMCS+CIVPFTR
Sbjct: 220 PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTR 279

Query: 324 GRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDGFSTI 383
           GRERSRPV SI+DEVR+L  QG+KEVTLLGQNVNS+ D +E      G+   LS GF+T 
Sbjct: 280 GRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSA-NLSRGFTTN 339

Query: 384 AKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQS 443
            K K+ GLRFS LLD++S   PEMR R+TSPHPKDFPDE L L+R+RHNICK IHLPAQS
Sbjct: 340 YKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQS 399

Query: 444 GNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSLVKEV 503
           G+S VL+ MRRGY+REAY+ LV  VR  IP V ++SDFI GFCGETE++H  T+SL++EV
Sbjct: 400 GSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREV 459

Query: 504 GYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGSVQLV 563
            Y+  ++FAYSMR+KT A+    DDVPEEVK RRL ELI  FR    +   + +G  QLV
Sbjct: 460 QYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLV 519

Query: 564 LVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQR-KAAIGDFVEVHISKSTR 623
           LVEG +KR+  T+L G++D   +V F +  V   D    G + +A  GD+V V I+ ++ 
Sbjct: 520 LVEGFSKRS-TTDLCGRNDANLKVIFPDAEV--EDITNPGLKVRAQPGDYVLVKITSASS 579

Query: 624 ASLFGEALAITKL 636
            +L G  L  T +
Sbjct: 580 QTLKGHILCRTTM 579

BLAST of Sed0014413 vs. ExPASy Swiss-Prot
Match: Q9VGZ1 (CDK5RAP1-like protein OS=Drosophila melanogaster OX=7227 GN=CG6345 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 5.7e-142
Identity = 257/500 (51.40%), Postives = 347/500 (69.40%), Query Frame = 0

Query: 144 RIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKVWQRL 203
           +++ E YGCQMN ND EVV SI+K  GY    + PE A++I + TCA+RD AEQ++  RL
Sbjct: 93  KVHFEVYGCQMNTNDTEVVFSILKENGYLRCQE-PEEADVIMLVTCAVRDGAEQRIRNRL 152

Query: 204 NYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDAYRDL 263
            +   +K +        RS  +HP ++ +LGCMAERLK+K+L+ ++ VDV+ GPD+Y+DL
Sbjct: 153 KHLRAMKNK--------RSTRRHPLQLTLLGCMAERLKEKLLEQEQCVDVIAGPDSYKDL 212

Query: 264 PRLLD-EVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIVPFT 323
           PRLL     YG   IN LLSL+ETYAD+ PVR++  S TAFVS+MRGC+NMC++CIVPFT
Sbjct: 213 PRLLAISRHYGNSAINVLLSLDETYADVMPVRLNSESPTAFVSIMRGCDNMCTYCIVPFT 272

Query: 324 RGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLS--DGF 383
           RGRERSRP+ SIV EV+ L  QGVKEVTLLGQNVNSY D    T  E   + K +   GF
Sbjct: 273 RGRERSRPLASIVAEVKALAEQGVKEVTLLGQNVNSYRD---RTAQEEQDSLKATPVPGF 332

Query: 384 STIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLP 443
           ST+ K K  G  F+ LL  ++   P+MR R+TSPHPKDF DE L ++RD  N+CK +HLP
Sbjct: 333 STVYKPKTGGTPFAALLRSVAQAVPDMRIRFTSPHPKDFSDEVLEVIRDHPNVCKQLHLP 392

Query: 444 AQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSLV 503
           AQSGN+ VLERMRRGY+REAYL+LV+ +R  +P+VG++SDFICGFCGETEEE  DT+SL+
Sbjct: 393 AQSGNTQVLERMRRGYSREAYLELVQHIRQFLPNVGLSSDFICGFCGETEEEFQDTVSLI 452

Query: 504 KEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGSV 563
           ++V Y++AY+FAYSMREKT AHR Y DDVP  VK  RL  +++ FR    + +    G  
Sbjct: 453 QQVQYNVAYLFAYSMREKTTAHRRYKDDVPINVKNERLQRMVQVFREGATQLHRKMEGQE 512

Query: 564 QLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVEVHISKS 623
           QL+L+EG +KR+ +    G++D   +V   ++ VP   GD   ++   +GDF+ V I +S
Sbjct: 513 QLILIEGKSKRS-DAHWFGRNDANIKVIVPSIYVP-ISGDSTARKSFGVGDFLAVRIEES 572

Query: 624 TRASLFGEALAITKLSSFYN 641
               L G  L ++ ++ F++
Sbjct: 573 NSQVLKGTPLELSNITDFHS 578

BLAST of Sed0014413 vs. ExPASy TrEMBL
Match: A0A6J1JMU5 (CDK5RAP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486642 PE=3 SV=1)

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 570/636 (89.62%), Postives = 594/636 (93.40%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRAS------SPP 80
           MG HVLRAPP  SST+IIFKPTSSASLLFKFGYA    +SPSSTR YFR S      SPP
Sbjct: 1   MGLHVLRAPPFSSSTSIIFKPTSSASLLFKFGYAF---NSPSSTRTYFRVSSLKYVASPP 60

Query: 81  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESV 140
               SIPL G FS R R F GGF+AA  AAPGSV KSEELRPS+SP+QSWVQRQNGIE +
Sbjct: 61  ---TSIPLSGYFSRRTRRFHGGFIAA-TAAPGSVKKSEELRPSVSPDQSWVQRQNGIEPL 120

Query: 141 PDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDN 200
             SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVD+PETAE+IFINTCAIRDN
Sbjct: 121 TGSEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDVPETAEVIFINTCAIRDN 180

Query: 201 AEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVV 260
           AEQKVWQRLNYFWFLKR+WKSNVA GRSQS+HPPKVVVLGCMAERLKDKILDADKMVDVV
Sbjct: 181 AEQKVWQRLNYFWFLKREWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDADKMVDVV 240

Query: 261 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMC 320
           CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCNNMC
Sbjct: 241 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNMC 300

Query: 321 SFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNW 380
           SFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYNDI +NTDVEPGTNW
Sbjct: 301 SFCIVPFTRGRERSRPVESIVSEVRELYREGVKEVTLLGQNVNSYNDIIDNTDVEPGTNW 360

Query: 381 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 440
           KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRD+HNIC
Sbjct: 361 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDKHNIC 420

Query: 441 KSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHA 500
           KSIHLPAQSGNS VLERMRRGYTRE YLDLV K+RSIIPDVGITSDFICGFCGETEEEHA
Sbjct: 421 KSIHLPAQSGNSTVLERMRRGYTREVYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 480

Query: 501 DTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYD 560
           DTLSL+KEVGYDMAYMFAYSMREKTHAHRNY+DDVPEEVKQRRLTELIETFRNSTG+CYD
Sbjct: 481 DTLSLIKEVGYDMAYMFAYSMREKTHAHRNYVDDVPEEVKQRRLTELIETFRNSTGQCYD 540

Query: 561 SEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVE 620
            +IGS+QLVLVEGPNKRA ETE+IGKSDRGHRVSFVNVA+PHRDGDYDGQRK AIGDFVE
Sbjct: 541 MQIGSIQLVLVEGPNKRALETEMIGKSDRGHRVSFVNVAIPHRDGDYDGQRKVAIGDFVE 600

Query: 621 VHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           V ISKSTRASLFGEALA+TKLSSFYN MDESIACVS
Sbjct: 601 VRISKSTRASLFGEALAVTKLSSFYNSMDESIACVS 629

BLAST of Sed0014413 vs. ExPASy TrEMBL
Match: A0A6J1EIA8 (CDK5RAP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111434491 PE=3 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 565/636 (88.84%), Postives = 589/636 (92.61%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFSKSSPSSTRPYFRAS------SPP 80
           MG HVL +PP  SST+IIFKPTSSASLLFKFGYA     SPSST  Y R S      SPP
Sbjct: 1   MGLHVLISPPFSSSTSIIFKPTSSASLLFKFGYAL--SKSPSSTTTYLRPSSLKYVASPP 60

Query: 81  KYLPSIPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESV 140
               SIPL G FS R R F GGF+AA  AAPGSV KSEELRPS+SP+QSWVQRQNGIE +
Sbjct: 61  ---TSIPLSGYFSRRTRRFHGGFIAA-TAAPGSVQKSEELRPSVSPDQSWVQRQNGIEPL 120

Query: 141 PDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDN 200
             SEVP+RGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVD+PETAE+IFINTCAIRDN
Sbjct: 121 TGSEVPLRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDVPETAEVIFINTCAIRDN 180

Query: 201 AEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVV 260
           AEQKVWQRLNYFWFLKR+WKSNVA GRSQS+HPPKVVVLGCMAERLKDKILDADKMVDVV
Sbjct: 181 AEQKVWQRLNYFWFLKREWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDADKMVDVV 240

Query: 261 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMC 320
           CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNS+TAFVSVMRGCNNMC
Sbjct: 241 CGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNMC 300

Query: 321 SFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNW 380
           SFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYND  +NTDVEPGTNW
Sbjct: 301 SFCIVPFTRGRERSRPVESIVSEVRELYREGVKEVTLLGQNVNSYNDTIDNTDVEPGTNW 360

Query: 381 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 440
           KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRD+HNIC
Sbjct: 361 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDKHNIC 420

Query: 441 KSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHA 500
           KSIHLPAQSGNS VLERMRRGYTRE YLDLV K+RSIIPDVGITSDFICGFCGETEEEHA
Sbjct: 421 KSIHLPAQSGNSTVLERMRRGYTREVYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 480

Query: 501 DTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYD 560
           DTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DDVPE+VKQRRLTELIETFRNSTG+CYD
Sbjct: 481 DTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDVPEDVKQRRLTELIETFRNSTGQCYD 540

Query: 561 SEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVE 620
            +IGS+QLVLVEGPNKRAPETE+IGKSDRGHRVSFVNVAVPHRDGDYDGQRK AIGDFVE
Sbjct: 541 MQIGSIQLVLVEGPNKRAPETEMIGKSDRGHRVSFVNVAVPHRDGDYDGQRKVAIGDFVE 600

Query: 621 VHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           V ISKSTRASLFGEALA+TKLSSFYN MDESIACVS
Sbjct: 601 VRISKSTRASLFGEALAVTKLSSFYNSMDESIACVS 630

BLAST of Sed0014413 vs. ExPASy TrEMBL
Match: A0A0A0KDY2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411190 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 558/637 (87.60%), Postives = 582/637 (91.37%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFS---KSSPSSTRPYFRASSPPKYL 80
           MGFHVLRAPPL SSTTI FKPTSS+S LFKF Y  +S     S SS+  YF  SS P Y+
Sbjct: 1   MGFHVLRAPPLSSSTTIFFKPTSSSSFLFKFRYPLYSPPFSKSSSSSISYFLPSS-PNYV 60

Query: 81  PS----IPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIES 140
            S    I L GSFS   R FRGGF+AA A A GSV +SEELRPSIS EQSWV  QNG ES
Sbjct: 61  ASPTTAILLSGSFSRSTRRFRGGFIAA-ATASGSVQRSEELRPSISSEQSWVLGQNGTES 120

Query: 141 VPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRD 200
           +P S+VP+ GR+YHETYGCQMNINDMEVVLSIMK AGYSETVD PETAEIIFINTCAIRD
Sbjct: 121 LPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRD 180

Query: 201 NAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDV 260
           NAEQKVWQRLNYFWFLKR WKSNVATGRSQS+HPPKVVVLGCMAERLKDKILD+DKMVDV
Sbjct: 181 NAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDV 240

Query: 261 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNM 320
           VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRI+KNS+TAFVSVMRGCNNM
Sbjct: 241 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNM 300

Query: 321 CSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTN 380
           CSFCIVPFTRGRERSRPVESIV EVRELYR+GVKEVTLLGQNVNSYND  EN D+EPGTN
Sbjct: 301 CSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTN 360

Query: 381 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 440
           WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI
Sbjct: 361 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 420

Query: 441 CKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEH 500
           CKSIHLPAQSGNS VLERMRRGYTREAYLDLV K+RSIIPDVGITSDFICGFCGETEEEH
Sbjct: 421 CKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEH 480

Query: 501 ADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCY 560
           ADTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DD+PEEVKQRRLTELIE FRNSTGRCY
Sbjct: 481 ADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCY 540

Query: 561 DSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFV 620
           DS+IGSVQLVLVEGPNKRAPETE+IGKSDR HRVSFVNVAVPHRDGDYDG RKA +GDFV
Sbjct: 541 DSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFV 600

Query: 621 EVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           EV ISKSTRASLFGEALAITKLSSFYNGMDE IAC+S
Sbjct: 601 EVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 635

BLAST of Sed0014413 vs. ExPASy TrEMBL
Match: A0A5D3CNB2 (CDK5RAP1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00300 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 558/637 (87.60%), Postives = 581/637 (91.21%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFS---KSSPSSTRPYFRASSPPKYL 80
           MGFHVLRAPP  SSTTIIFKPTSS   LFKF YA +S     S SS+  YF  SS P Y+
Sbjct: 1   MGFHVLRAPPFSSSTTIIFKPTSS---LFKFRYALYSPPFSKSSSSSISYFLPSS-PNYV 60

Query: 81  PS----IPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIES 140
            S    IPL GSFS   R FRGGF+AA  A+ GSV + EELRPSIS EQSWV RQNG ES
Sbjct: 61  ASPTTAIPLSGSFSRSTRRFRGGFIAATTAS-GSVQRLEELRPSISTEQSWVLRQNGTES 120

Query: 141 VPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRD 200
           +P S+VP RGR+YHETYGCQMNINDMEVVLSIMK AGY ETVD PETAEIIFINTCAIRD
Sbjct: 121 LPASDVPSRGRVYHETYGCQMNINDMEVVLSIMKKAGYGETVDTPETAEIIFINTCAIRD 180

Query: 201 NAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDV 260
           NAEQKVWQRLNYFWFLKRQWKSNVA GRSQS+HPPKVVVLGCMAERLKDKILD+DKMVDV
Sbjct: 181 NAEQKVWQRLNYFWFLKRQWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDV 240

Query: 261 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNM 320
           VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRI+KNS+TAFVSVMRGCNNM
Sbjct: 241 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNM 300

Query: 321 CSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTN 380
           CSFCIVPFTRGRERSRPVESIV EV+ELYR+GVKEVTLLGQNVNSYND  EN D+EPGTN
Sbjct: 301 CSFCIVPFTRGRERSRPVESIVCEVKELYREGVKEVTLLGQNVNSYNDTTENADIEPGTN 360

Query: 381 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 440
           WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI
Sbjct: 361 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 420

Query: 441 CKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEH 500
           CKSIHLPAQSGNS VLERMRRGYTREAYLDLV K+RSIIPDVGITSDFICGFCGETEEEH
Sbjct: 421 CKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEH 480

Query: 501 ADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCY 560
           ADTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DD+PEEVKQRRLTELIE FRNSTGRCY
Sbjct: 481 ADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCY 540

Query: 561 DSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFV 620
           DS+IGSVQLVLVEGPNKRAPETE+IGKSDR HRVSFVNVAVPHRDGDYDG RKA +GDFV
Sbjct: 541 DSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAVVGDFV 600

Query: 621 EVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           EV ISKSTRASLFGEALAITKLSSFYN MDESIACVS
Sbjct: 601 EVRISKSTRASLFGEALAITKLSSFYNRMDESIACVS 632

BLAST of Sed0014413 vs. ExPASy TrEMBL
Match: A0A1S3BLE1 (CDK5RAP1-like protein OS=Cucumis melo OX=3656 GN=LOC103491318 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 558/637 (87.60%), Postives = 581/637 (91.21%), Query Frame = 0

Query: 21  MGFHVLRAPPLCSSTTIIFKPTSSASLLFKFGYAAFS---KSSPSSTRPYFRASSPPKYL 80
           MGFHVLRAPP  SSTTIIFKPTSS   LFKF YA +S     S SS+  YF  SS P Y+
Sbjct: 1   MGFHVLRAPPFSSSTTIIFKPTSS---LFKFRYALYSPPFSKSSSSSISYFLPSS-PNYV 60

Query: 81  PS----IPLYGSFSSRARCFRGGFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIES 140
            S    IPL GSFS   R FRGGF+AA  A+ GSV + EELRPSIS EQSWV RQNG ES
Sbjct: 61  ASPTTAIPLSGSFSRSTRRFRGGFIAATTAS-GSVQRLEELRPSISTEQSWVLRQNGTES 120

Query: 141 VPDSEVPIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRD 200
           +P S+VP RGR+YHETYGCQMNINDMEVVLSIMK AGY ETVD PETAEIIFINTCAIRD
Sbjct: 121 LPASDVPSRGRVYHETYGCQMNINDMEVVLSIMKKAGYGETVDTPETAEIIFINTCAIRD 180

Query: 201 NAEQKVWQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDV 260
           NAEQKVWQRLNYFWFLKRQWKSNVA GRSQS+HPPKVVVLGCMAERLKDKILD+DKMVDV
Sbjct: 181 NAEQKVWQRLNYFWFLKRQWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDV 240

Query: 261 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNM 320
           VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRI+KNS+TAFVSVMRGCNNM
Sbjct: 241 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNM 300

Query: 321 CSFCIVPFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTN 380
           CSFCIVPFTRGRERSRPVESIV EV+ELYR+GVKEVTLLGQNVNSYND  EN D+EPGTN
Sbjct: 301 CSFCIVPFTRGRERSRPVESIVCEVKELYREGVKEVTLLGQNVNSYNDTTENADIEPGTN 360

Query: 381 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 440
           WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI
Sbjct: 361 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNI 420

Query: 441 CKSIHLPAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEH 500
           CKSIHLPAQSGNS VLERMRRGYTREAYLDLV K+RSIIPDVGITSDFICGFCGETEEEH
Sbjct: 421 CKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEH 480

Query: 501 ADTLSLVKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCY 560
           ADTLSL+ EVGYDMAYMFAYSMREKTHAHRNY+DD+PEEVKQRRLTELIE FRNSTGRCY
Sbjct: 481 ADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCY 540

Query: 561 DSEIGSVQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFV 620
           DS+IGSVQLVLVEGPNKRAPETE+IGKSDR HRVSFVNVAVPHRDGDYDG RKA +GDFV
Sbjct: 541 DSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAVVGDFV 600

Query: 621 EVHISKSTRASLFGEALAITKLSSFYNGMDESIACVS 651
           EV ISKSTRASLFGEALAITKLSSFYN MDESIACVS
Sbjct: 601 EVRISKSTRASLFGEALAITKLSSFYNRMDESIACVS 632

BLAST of Sed0014413 vs. TAIR 10
Match: AT4G36390.1 (Methylthiotransferase )

HSP 1 Score: 834.3 bits (2154), Expect = 6.5e-242
Identity = 433/625 (69.28%), Postives = 495/625 (79.20%), Query Frame = 0

Query: 25  VLRAPPLCSSTTIIFKPTSSASLLFKF-GYAAFSKSSPSSTRPYFRASSPPKYLPSIPL- 84
           +L  P  CS   + FK ++      +F    A   SS SS+    R  S    L   P+ 
Sbjct: 12  ILSNPHGCS---LCFKASTQRCFALRFLSSKATHASSSSSSALLPRCRSSTHRLTQKPIN 71

Query: 85  --YGSFSSRARCFRGGFVAAMAAAPG-SVHKSEELRPSISPEQSWVQRQNGIESVPDSEV 144
              G   + +R F    +A+     G S+H   +   +     S    +   ES  DS++
Sbjct: 72  RKKGFSLNLSRSFSISQIASSGKFDGPSLH---QFVSNAQAHASLTTPETESESTLDSDI 131

Query: 145 PIRGRIYHETYGCQMNINDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKV 204
             +GRIYHETYGCQMNINDME+VL+IMKN+GY E V  PE+AE+IF+NTCAIR+NAEQ+V
Sbjct: 132 ASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNTCAIRENAEQRV 191

Query: 205 WQRLNYFWFLKRQWKSNVATGRSQSQHPPKVVVLGCMAERLKDKILDADKMVDVVCGPDA 264
           WQRLNYFWFLKR+WK N ATGR++S  PPKVVVLGCMAERLKDKILD+DKMVDVVCGPDA
Sbjct: 192 WQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDA 251

Query: 265 YRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIV 324
           YRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRIS+NSITAFVSVMRGCNNMC+FCIV
Sbjct: 252 YRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAFCIV 311

Query: 325 PFTRGRERSRPVESIVDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDG 384
           PFTRGRERSRPVESI+ EV EL+  GVKEVTLLGQNVNSYND  ++ D E G NW+ S+G
Sbjct: 312 PFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYND--DSADRESGANWEYSEG 371

Query: 385 FSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHL 444
           FS+  KVK MGLRF+DLLDRLS EFPEMRFR+TSPHPKD+PDE LYLMRDRHNIC  IHL
Sbjct: 372 FSSRCKVKNMGLRFADLLDRLSVEFPEMRFRFTSPHPKDYPDELLYLMRDRHNICNLIHL 431

Query: 445 PAQSGNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSL 504
           PAQSGNS +LE+MRRGYTREAYLDLV+K+RSIIPDV ITSDFI GFCGETEEEH +TLSL
Sbjct: 432 PAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITSDFITGFCGETEEEHQETLSL 491

Query: 505 VKEVGYDMAYMFAYSMREKTHAHRNYIDDVPEEVKQRRLTELIETFRNSTGRCYDSEIGS 564
           V+ VGYDMAYMFAYSMREKTHAHRNY DDVPEEVKQRRLTELI+ FR +TG CYDS++GS
Sbjct: 492 VRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLTELIDAFRETTGPCYDSQVGS 551

Query: 565 VQLVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHR----DGDYDGQRKAAIGDFVEV 624
            QLVLVEGPNKRAPETELIGK+D+GHRVSFV   +  +    DGD D +R   IGDFVEV
Sbjct: 552 TQLVLVEGPNKRAPETELIGKTDKGHRVSFVTKPLFDKACLLDGD-DLKRNPGIGDFVEV 611

Query: 625 HISKSTRASLFGEALAITKLSSFYN 641
            I KSTRASLFGEALAI+K+S F++
Sbjct: 612 QIEKSTRASLFGEALAISKMSLFHD 627

BLAST of Sed0014413 vs. TAIR 10
Match: AT1G72090.1 (Methylthiotransferase )

HSP 1 Score: 143.3 bits (360), Expect = 7.0e-34
Identity = 128/549 (23.32%), Postives = 235/549 (42.81%), Query Frame = 0

Query: 96  GFVAAMAAAPGSVHKSEELRPSISPEQSWVQRQNGIESVPDSEVPIRGRIYHETYGCQMN 155
           GF   + A   +   ++  R S  P+Q     ++ + + P  ++P    IY +T+GC  N
Sbjct: 18  GFRLPLNAVGINPKTNKSKRISSKPDQITASNRDSL-APPSMKIPGTQTIYIKTFGCSHN 77

Query: 156 INDMEVVLSIMKNAGYSETVDIPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKS 215
            +D E +   +   GY+ T ++PE A++  INTC ++  ++  +               +
Sbjct: 78  QSDSEYMAGQLSAFGYALT-EVPEEADLWLINTCTVKSPSQSAM--------------ST 137

Query: 216 NVATGRSQSQHPPKVVVLGCMAERLKD-KILDADKMVDVVCGPDAYRDLPRLLDEVDYGQ 275
            +  GRS  +    +V+ GC+ +  +D K L+   +V V       + + R+++ V+   
Sbjct: 138 LITRGRSGKK---PLVIAGCVPQGSRDLKELEGVSVVGV-------QQIDRVVEIVEETL 197

Query: 276 KGINTLLSLEETYADISPVRISKNSITAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESI 335
           KG    L   +T   +   ++ +N+    + +  GC   C++C     RG   S  V+S+
Sbjct: 198 KGHEVRLLTRKTLPALDLPKVRRNNFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 257

Query: 336 VDEVRELYRQGVKEVTLLGQNVNSYNDIAENTDVEPGTNWKLSDGFSTIAKVKKMGLRFS 395
           V+ VR +  +GVKE+ L  ++  +Y                           + +G+   
Sbjct: 258 VERVRTVISEGVKEIWLSSEDTGAYG--------------------------RDIGVNLP 317

Query: 396 DLLDRLSTEFPE-----MRFRYTSP-----HPKDFPDEFLYLMRDRHN-ICKSIHLPAQS 455
            LL+ +  E P      +R   T+P     H K+           RH  +   +H+P QS
Sbjct: 318 ILLNAIVKELPSDQSTMLRIGMTNPPFILEHLKEIAAVL------RHPCVYTFLHVPVQS 377

Query: 456 GNSAVLERMRRGYTREAYLDLVRKVRSIIPDVGITSDFICGFCGETEEEHADTLSLVKEV 515
           G+ +VL  M R YT   +  +V  +  ++P + I +D ICGF GET+E+ + T+ L+K+ 
Sbjct: 378 GSDSVLTAMNREYTASEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVELIKDY 437

Query: 516 GYDMAYMFAYSMREKTHAHR-NYIDDVPEEVKQRRLTELIETFRNSTG-RCYDSEIGSVQ 575
            +   ++  +  R  T A +   +     + + R LT + E F   TG  C +  I   +
Sbjct: 438 KFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSRELTSVFEAFAPYTGMECREERIWITE 487

Query: 576 LVLVEGPNKRAPETELIGKSDRGHRVSFVNVAVPHRDGDYDGQRKAAIGDFVEVHISKST 631
            V  +G         L+     GH   +V V V   +        + +G      I+   
Sbjct: 498 -VATDG-------IHLV-----GHTKGYVQVLVTGPE--------SMLGTSAMARITSVG 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887766.10.0e+0090.30CDK5RAP1-like protein isoform X2 [Benincasa hispida][more]
XP_022989605.10.0e+0089.62CDK5RAP1-like protein [Cucurbita maxima][more]
XP_038887765.10.0e+0087.96CDK5RAP1-like protein isoform X1 [Benincasa hispida][more]
XP_022927671.10.0e+0088.84CDK5RAP1-like protein [Cucurbita moschata][more]
XP_023529365.10.0e+0088.52CDK5RAP1-like protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8H0V19.2e-24169.28CDK5RAP1-like protein OS=Arabidopsis thaliana OX=3702 GN=At4g36390 PE=2 SV=1[more]
Q2R1U41.3e-23972.44CDK5RAP1-like protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0592800 P... [more]
Q9JLH62.5e-14553.85Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Rattus norvegicus OX=10116 ... [more]
Q8BTW85.6e-14554.97Mitochondrial tRNA methylthiotransferase CDK5RAP1 OS=Mus musculus OX=10090 GN=Cd... [more]
Q9VGZ15.7e-14251.40CDK5RAP1-like protein OS=Drosophila melanogaster OX=7227 GN=CG6345 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JMU50.0e+0089.62CDK5RAP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486642 PE=3 SV=1[more]
A0A6J1EIA80.0e+0088.84CDK5RAP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111434491 PE=3 SV=1[more]
A0A0A0KDY20.0e+0087.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411190 PE=3 SV=1[more]
A0A5D3CNB20.0e+0087.60CDK5RAP1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49... [more]
A0A1S3BLE10.0e+0087.60CDK5RAP1-like protein OS=Cucumis melo OX=3656 GN=LOC103491318 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G36390.16.5e-24269.28Methylthiotransferase [more]
AT1G72090.17.0e-3423.32Methylthiotransferase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 300..540
e-value: 4.7E-55
score: 198.9
IPR002792TRAM domainPFAMPF01938TRAMcoord: 555..630
e-value: 4.6E-14
score: 52.2
IPR002792TRAM domainPROSITEPS50926TRAMcoord: 554..631
score: 14.044373
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 304..500
e-value: 2.1E-36
score: 125.8
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 296..551
score: 36.297146
IPR038135Methylthiotransferase, N-terminal domain superfamilyGENE3D3.40.50.12160coord: 144..285
e-value: 2.2E-38
score: 132.9
NoneNo IPR availableTIGRFAMTIGR01574TIGR01574coord: 145..628
e-value: 4.1E-116
score: 386.5
NoneNo IPR availableSFLDSFLDF00413CDK5RAP1coord: 144..635
e-value: 0.0
score: 679.5
NoneNo IPR availableSFLDSFLDG01082B12-binding_domain_containingcoord: 221..520
e-value: 7.1E-30
score: 98.7
NoneNo IPR availablePIRSRPIRSR000167-1PIRSR000167-1coord: 438..529
e-value: 7.6E-4
score: 16.7
NoneNo IPR availablePANTHERPTHR43020CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1coord: 125..641
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 308..525
e-value: 5.70657E-9
score: 54.6469
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 294..547
IPR013848Methylthiotransferase, N-terminalPFAMPF00919UPF0004coord: 144..256
e-value: 4.5E-30
score: 103.6
IPR013848Methylthiotransferase, N-terminalPROSITEPS51449MTTASE_Ncoord: 143..272
score: 37.14101
IPR023404Radical SAM, alpha/beta horseshoeGENE3D3.80.30.20tm_1862 like domaincoord: 298..554
e-value: 3.4E-82
score: 277.5
IPR005839MethylthiotransferaseTIGRFAMTIGR00089TIGR00089coord: 144..627
e-value: 1.1E-113
score: 378.3
IPR005839MethylthiotransferaseSFLDSFLDG01061methylthiotransferasecoord: 144..635
e-value: 0.0
score: 679.5
IPR006463tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaBSFLDSFLDF00273(dimethylallyl)adenosine_tRNAcoord: 144..631
e-value: 0.0
score: 538.9
IPR020612Methylthiotransferase, conserved sitePROSITEPS01278MTTASE_RADICALcoord: 304..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0014413.1Sed0014413.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity
biological_process GO:0035600 tRNA methylthiolation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0035597 N6-isopentenyladenosine methylthiotransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0035596 methylthiotransferase activity
molecular_function GO:0016740 transferase activity