Sed0013495 (gene) Chayote v1

Overview
NameSed0013495
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRHOMBOID-like protein 12
LocationLG08: 35251344 .. 35256422 (-)
RNA-Seq ExpressionSed0013495
SyntenySed0013495
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAGAGAAAAAAGTGGAATTAAATCCCATAATTGATCCATTGAAGCCACAATCACTCCAAGTATAAACACCTGTGTTCATCGACTCATTGAAGAAATGAAATAGTACCTCCCACCAACCACAGCTATCTCATAGACCGAGCCATAATCAAGATCAACAGAAACATGAATAAACTTTTTGTTCGTCAACTTGCATCACGGCTTTCAAATTCCTTCGCTTCTTCTCATCCTTGCAAGGATTGCGCTTCTTTTGCCACATTAGTGCGAACCCACAACCAAAACCGCCTGTTTGGCTCATTCCTCACTCGCCCACTGCAGGATCGCTCGCGTTCATGGAGTTCACATCGAAGTTTCGCGGAGAAATTTCATGGGTTTTTCTCACGGCCGGTGATCGGAAATGGGTTCTTGTTGGATCCTTCAAATACCCTTTTCAAGAGGTCGATGAAGAGTCTAGTTGAAAACAGGTTTCTCTTTACGGGAGGTCAATTTTCGAAAATGAGGTTTCAGTTGAAACCGGGCTCCGATTATCAATGGCGTGGCAGGTAATGTGTTTGTTTTGTTTGGAATTTTGGTGGTTTTATTCGAAGAATGTGAAGGCCTTGTTTGATTGAGTAATTTCATTCAGCACTAATAATTTCATTTTCGATGGTAAATGCGAACATTCCCAATTGCATTTTCCTGGTTATATTTGGGGAAGTCCTTATTTCATTGTTGCTATTCACAGAACCCGGTTTCAAAGGTGGTTTCAAGGGCTAACGACTAATGATGTGGTGTTGGGCTTGATTATAGCTAATGTTGGTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTGTAAGTACACAACTGCAATTACATGCCTCAGAATTGTAGGCACAGTGAGACATTGTTTCTTCTCGACTCCCACTTTAGGAGTGGTACAGTGGTTGAAGACTTGGGCTTTGAAAGTATGCTCTCTTCAAGGTTCGAGACTCATCTGTGCATTAATCCTTCGAAGTCTCTCAGTGCCTAGTCTAGGGACGGGCGTGGTTACCCTTGTTTCAAAAAAAAAAACTTTGCTTGGGATCCACTTTCTCTTGGATTGAGAGACTACTGATGTTCTTTTTTTTGTATCCGTGACTATTTGGGTCAGCTTGCGCACACCCGACTAATGTCACAGGACATATCATCTGACCCTACAACATTTGGATGTCATGGAAACATGTAGGAAATTAATTCCTAGGTAGGTGGTCACCATGGATTGAACTCATCACCTTTAGCCTGAGAATCAGAATTTATGGCAATCAATAATAATGCCTAGAAATCTAGATATATGGGTAATTGCTAGAAATCAAGAACAATCAGCTATACTCGTGACAATCTTGTGAAGATGGTTGAAAGTATTAGATAACTATATTTTTCTGTATACTCTTATATTCTTTCATTTCTTACCTTGAAATCTTGGCTTATTATAAAAATAAACAAATAAAATCATCTTTGTTCTACTAATGCCTCTATAATATGAATTGAGACGATTTAGATTAACAATTGGATGGTTGAAAACTCACCCCGTATTCTATGTGAGTCGGTAACAATCCTTATTAAGTAATGGGTTCTTAAGAAGGCTTGCTTACATGTCAAATTTCATCTTGACTTGATAACTAGAATCGTCTCCCAACCTAGCTCTACCTGGCCAATTATATGTTTAAAACCCATCTCATTTCATGAGTATTCAAATCACCCCTTCATTGGCATCTAGGTTGTCCAAACAAACCTGCCGCTTAGTAAACTACATTTCTTGTAAGAGAAACATTGGAGTTGAGATCACCTCGCGGACATTCAGGATTTCTCAAGATGTATGGCCTCTAAAAGATGTGATCAACTTAACATAGTTTTCGTTTTCATTTTCATTTTTTTTTTCAAATTGATTAACATGTTTTGAGAAATATCTGTGATAAAGTCTTACATGCCATTTTGGAAGTTTTAAGTTTCCAAGCTTGGAACTTCAATTCAATTGGTCTCTCTGTTTGTGACTATATTTTCGATGTTTCACTGCTGTATTCACAGATCTCTGTAGAAAATGTTAGAAGTGGCCGCATACATACAATGATTACCTCTGCCTTCAGTCACGTAGACATTGGCCATATAGTATCCAACATGATTGGACTATATTTCTTTGGGATGAACGTATGGCTATCTTTCTTCGAACTTCTATTGGACCCTGATTTTTATATATTTTATTCTCTCATCTGTTTATATGTGGGTGGCTTATGTAAAATTTCTTGTTTACTTTCCTCTTTTTAGCTTATATTCTAATCTTGTAATTTTTGTCTTTGGACACACAATCTTGTACTTGTAATTATTTATATTCAGCCTTTCCCTTAGTTATTATCTCATTTTGAGGCTGCTATGGTAGTATTATATATTTTAAAAACCAAATTTGACTATATTTTTTAAAGTTAAGCCTTTTGAGTGAAAATAATTTTGAGGAAACTTGCTTCAATAAGCATTGATTAATTAATATTATGATGAGTACCTCATACGCAAATTTTCGAGTTGATACTGGATTTGGAGTAATATACTTTGATTCAAGTAATTATAGGGTGAGTTGGGGTGTCTTTTGGATATGTAAAAATTGTTTAAGCCAATTAAAAAAAAATCAAAATATGCATACAGTATGGTTGAATTGTCGAAAATAATTTTAGATGTTCTGATAGTTCTTCCAGAAGTTGTATGCATCTAGTCTACTTGTGAACTATAAAGTTTACCTTTTCTGTTTTGCCGTGGTTTTTCCAGAACATAATAATGACCTGTTAGTGTTAAAACTTACCTCATTCTCAGCCCTGATAGAATTGACACTTGATCTGCGTTGTTTGAGCTCATATATTTTTCCTTTAACAGATCGGCAGAGTATTTGGTCCTCAGTTTTTGTTGAAATTGTATCTGGCTGGGGCAATTGGTGGGTCAGCGTTTTACTTGGCGCATCATCTGTTCCAAGCATCATCCTCAAAGGTAGGCAAATTTTTTAGACACTCGTTTTGCTTTTTGTAATCATGAATGACAATCTATTTGATTTATGATGTTTCGTTAAGTTTTCATTCATTTATGAAAGTAGTTTCAGTTTCCTGTCAAAAAAAGAAAATAATAATAATAATAATAATAAGATATAAAGTTCTCCTAGGGATGACACAGTTGTTGAAGACTTGGGCTTTGAGGGTATGCTCCTCTCAAGGTCTTGGATTCAAAACTCAATTGTGACAATATTGATATCTCCAGTGCTTGGCTTAGGGACGGGCGTGGTTACCTTGTTTCAAAAAAAATAAAAATAATAAGATATAAAGGCAGTATCAGAATTTTATTTAGATGTTAATTATGCTTTATTTGCTCAACTAGAATCAAAGCTCGATTTGGTAACTTTTTGGTGGTGCTTTTGGTTAGGTATCCAAATACCTAATGGAACTTGTACCAAACTGCAAGAACTCCTAGCACAATATTGATGGAAAATAATGATAGTTCTTCGCTAACATTAACAGGACAGAAATGAACTACAAACCTGCTGTGAATTCAAGAGCATTGGAACCTCTCCTCCAAGTCCTAAAACTCTACCAAAACACACTATCCATGCCTCCCTCTCCCCTACCCTCAACACTTATTTATATCCATGCTTTCTCTCTTTCAACACAAAATAACCCCCACAACACACTATCCACTTGTCCCATCTCCAATACCTGTTTTAACCTCTAGAACATATAGGAGTATTTGGGGGTCAAACACTTTCATAATGGTAGAAGAGAAGTTCAGCTGTCAATGGAATAAAACATCTCGGCATAGAACTTTTCTTGTTATATTAATATGGGATATTTGTTGGACAGTATAAACTGAATTTTTAAACAGTTTTTGTTTACTCATTTTGTTCTTTCAACAGGGGCGGTCATTGTGGGGCAAGGACCCGGCACGGGCACAAGGATTGGTATGCTTTTGAAATTCCATATCTACTTAGTTTGTTACCTTTTCAAATGTAGAAGTCAAAATTTGAACCATATGCTCTGTGTGAATTGTTTTCTTAGTGGTTTGATCTGAGGCTTTGTTCCTTTCATCTTACATTGTACCAATCATCTTTGACTTACAAGAATCAGGATACTCGTTAGAACTTCAATCATCAACTCTATTCATTTTGTTTGATCGATTAGGGGGCGAGCGGAGCCGTGAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCGACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGTAAGCTCAAATCTAATTAGCACATAGATTCACAGTTCATAATGAAAAATCCTGATATAGTCCCATTGTGGTTTCAATACAGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGGTATAATGTTTAATCTAATAACTTTAAGTCCTATTTGATAATCAGGTAGTTTTTTTTTTGTTTTTGAAAACTAAGTTCATAAATGCTACTTCCACTTGCGAGTTTCTTTGTTTTATAAAACTTGTTTTCATTTTGGAATTTGGCTAATAATTCAAATTTTTTCGTAAGAATGATGTAAATCATGGTAAAGAATTTGTGATACAAAGGAAAATTTTGAAAAAACAAATAATTAGCAAACGAGTATAAACTTCTCTATACAACTTCATCTTCAGTTTTTTTTTCTCTGTTGCATATGTCTGGCATTCTACTACTGATTGACAAATTCATGAAACATGTTTGATCTCTGCAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGTGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGCTTCTGATTGTCGGTTGAACTTTTGTCCTTGCAATCCAGCTTCTAGTTCAAGACAACTCCTATTCTGATCCCCTCTCACTCTATGTTAGAGTATTAGACTATAGTTTAGTTAATATTAGTATTTAGTATATAGAAAAACTTTGAATAGTTGTGGAGTTGTTGAAGACATCTAAATTATTGTTGTGTACAAAATTTGGAGCTGTTGAAGACATCTAAATTATTGTTG

mRNA sequence

AAAAAAAAGAGAAAAAAGTGGAATTAAATCCCATAATTGATCCATTGAAGCCACAATCACTCCAAGTATAAACACCTGTGTTCATCGACTCATTGAAGAAATGAAATAGTACCTCCCACCAACCACAGCTATCTCATAGACCGAGCCATAATCAAGATCAACAGAAACATGAATAAACTTTTTGTTCGTCAACTTGCATCACGGCTTTCAAATTCCTTCGCTTCTTCTCATCCTTGCAAGGATTGCGCTTCTTTTGCCACATTAGTGCGAACCCACAACCAAAACCGCCTGTTTGGCTCATTCCTCACTCGCCCACTGCAGGATCGCTCGCGTTCATGGAGTTCACATCGAAGTTTCGCGGAGAAATTTCATGGGTTTTTCTCACGGCCGGTGATCGGAAATGGGTTCTTGTTGGATCCTTCAAATACCCTTTTCAAGAGGTCGATGAAGAGTCTAGTTGAAAACAGGTTTCTCTTTACGGGAGGTCAATTTTCGAAAATGAGGTTTCAGTTGAAACCGGGCTCCGATTATCAATGGCGTGGCAGAACCCGGTTTCAAAGGTGGTTTCAAGGGCTAACGACTAATGATGTGGTGTTGGGCTTGATTATAGCTAATGTTGGTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCTCTGTAGAAAATGTTAGAAGTGGCCGCATACATACAATGATTACCTCTGCCTTCAGTCACGTAGACATTGGCCATATAGTATCCAACATGATTGGACTATATTTCTTTGGGATGAACATCGGCAGAGTATTTGGTCCTCAGTTTTTGTTGAAATTGTATCTGGCTGGGGCAATTGGTGGGTCAGCGTTTTACTTGGCGCATCATCTGTTCCAAGCATCATCCTCAAAGGGGCGGTCATTGTGGGGCAAGGACCCGGCACGGGCACAAGGATTGGGGGCGAGCGGAGCCGTGAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCGACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGTGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGCTTCTGATTGTCGGTTGAACTTTTGTCCTTGCAATCCAGCTTCTAGTTCAAGACAACTCCTATTCTGATCCCCTCTCACTCTATGTTAGAGTATTAGACTATAGTTTAGTTAATATTAGTATTTAGTATATAGAAAAACTTTGAATAGTTGTGGAGTTGTTGAAGACATCTAAATTATTGTTGTGTACAAAATTTGGAGCTGTTGAAGACATCTAAATTATTGTTG

Coding sequence (CDS)

ATGAATAAACTTTTTGTTCGTCAACTTGCATCACGGCTTTCAAATTCCTTCGCTTCTTCTCATCCTTGCAAGGATTGCGCTTCTTTTGCCACATTAGTGCGAACCCACAACCAAAACCGCCTGTTTGGCTCATTCCTCACTCGCCCACTGCAGGATCGCTCGCGTTCATGGAGTTCACATCGAAGTTTCGCGGAGAAATTTCATGGGTTTTTCTCACGGCCGGTGATCGGAAATGGGTTCTTGTTGGATCCTTCAAATACCCTTTTCAAGAGGTCGATGAAGAGTCTAGTTGAAAACAGGTTTCTCTTTACGGGAGGTCAATTTTCGAAAATGAGGTTTCAGTTGAAACCGGGCTCCGATTATCAATGGCGTGGCAGAACCCGGTTTCAAAGGTGGTTTCAAGGGCTAACGACTAATGATGTGGTGTTGGGCTTGATTATAGCTAATGTTGGTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCTCTGTAGAAAATGTTAGAAGTGGCCGCATACATACAATGATTACCTCTGCCTTCAGTCACGTAGACATTGGCCATATAGTATCCAACATGATTGGACTATATTTCTTTGGGATGAACATCGGCAGAGTATTTGGTCCTCAGTTTTTGTTGAAATTGTATCTGGCTGGGGCAATTGGTGGGTCAGCGTTTTACTTGGCGCATCATCTGTTCCAAGCATCATCCTCAAAGGGGCGGTCATTGTGGGGCAAGGACCCGGCACGGGCACAAGGATTGGGGGCGAGCGGAGCCGTGAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCGACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGTGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGCTTCTGA

Protein sequence

MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSHRSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDYQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRIHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLFQASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Homology
BLAST of Sed0013495 vs. NCBI nr
Match: XP_038893963.1 (RHOMBOID-like protein 12, mitochondrial [Benincasa hispida])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 293/339 (86.43%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNKLF R+LASRLSNSFA+S  CKDCASFATLVRTHNQN LF S LTRPLQDRSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSFANSQHCKDCASFATLVRTHNQNHLFASSLTRPLQDRSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSFAEK HGF S  V+G  F+L+PSNTLFK S+KSLVE+  LFTG  FSK+RFQLKPG D
Sbjct: 61  RSFAEKIHGFSSVAVVGKRFILNPSNTLFKGSIKSLVESSSLFTGTPFSKLRFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
            QW GR   Q WFQ LT NDVVLGLIIANV VFLLW+IADPIFMSKNFTIS+ENVRSGRI
Sbjct: 121 RQWGGR---QSWFQRLTPNDVVLGLIIANVAVFLLWQIADPIFMSKNFTISIENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSH +IGHIVSNMIGLYFFGMNIGRVFGP+FLLKLYLAGAIGGSAFYLAH+LF
Sbjct: 181 HTLITSAFSHKEIGHIVSNMIGLYFFGMNIGRVFGPEFLLKLYLAGAIGGSAFYLAHNLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK R  WGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sed0013495 vs. NCBI nr
Match: XP_022137034.1 (RHOMBOID-like protein 12, mitochondrial [Momordica charantia])

HSP 1 Score: 562.0 bits (1447), Expect = 3.5e-156
Identity = 282/339 (83.19%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNKLF  +LASR+SNS A+S PCK+C SFATLVRTHNQN LFGSF++RPLQD S SWSS 
Sbjct: 1   MNKLFSLKLASRISNSTATSRPCKNCTSFATLVRTHNQNHLFGSFISRPLQDHSHSWSSQ 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSFAEK HG FS  V+G  FLL+ SNTLFK S++SL+E RFLFTG QFSK+RFQLKPG D
Sbjct: 61  RSFAEKIHGLFSVSVVGKRFLLNSSNTLFKGSIRSLIERRFLFTGAQFSKLRFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           +QWRGR   + WFQ LT+NDVVLGLIIANV VFLLWRIADP+FMS+NFTISVEN+RSGR+
Sbjct: 121 FQWRGR---RSWFQRLTSNDVVLGLIIANVAVFLLWRIADPVFMSRNFTISVENIRSGRV 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV++GHIVSNMIGLYFFGMNIGRVFG +FLL+LYLAGAIGGSAFYL HHLF
Sbjct: 181 HTLITSAFSHVEVGHIVSNMIGLYFFGMNIGRVFGSEFLLRLYLAGAIGGSAFYLVHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK R  WGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSKDRPFWGKDPIRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 336

BLAST of Sed0013495 vs. NCBI nr
Match: XP_022923619.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita moschata])

HSP 1 Score: 558.1 bits (1437), Expect = 5.1e-155
Identity = 285/339 (84.07%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNK+F R+LASRLSNS+ +SH C+DCASFAT VRT NQN  +GSF+TRP++D SRSWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSF EK HGFFS PV+G  F+L+PSNTLFK S+KSLV  R LF G  FSK++FQLKPG D
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           ++W GR   + WFQ LTTNDVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HRWSGR---RAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV+IGHIVSNMIGLYFFGMNIGRVFGP+FLL LYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSS+ R  WGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sed0013495 vs. NCBI nr
Match: KAG7019987.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557.0 bits (1434), Expect = 1.1e-154
Identity = 285/339 (84.07%), Postives = 306/339 (90.27%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNK F R+LASRLSNS+ +SH C+DCASFAT VRT NQN  +GSF+TRP++D SRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSF EK HGFFS PV+G  F+L+PSNTLFK S+KSLV  R LF G  FSK++FQLKPG D
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           ++W GR   + WFQ LTTNDVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HRWPGR---RAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV+IGHIVSNMIGLYFFGMNIGRVFGP+FLL LYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSS+ R  WGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sed0013495 vs. NCBI nr
Match: XP_023001193.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita maxima])

HSP 1 Score: 555.8 bits (1431), Expect = 2.5e-154
Identity = 285/339 (84.07%), Postives = 306/339 (90.27%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNK F R+LASRLSNS+ +SH C+DCASFAT VRT NQN  +GSF+TRP++D SRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RS  EK HGFFS PV+G  F+L+PS+TLFK S+KSLVE R LF G  FSK++FQLKPG D
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVE-RSLFMGSPFSKLKFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           ++W GR   + WFQ LTTNDVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HRWPGR---RAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV+IGHIVSNMIGLYFFGMNIGRVFGP+FLL LYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK R  WGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSKSRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sed0013495 vs. ExPASy Swiss-Prot
Match: Q9FZ81 (RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-89
Identity = 192/349 (55.01%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 2   NKLFVRQLASRLSNSFA--SSHPCKDCASFATLVRTHNQNRLF------GSFLTRPLQ-- 61
           N+  V   +SRL+   A  ++H   +  +F +L +  NQ+R F       S  + P+   
Sbjct: 6   NRRVVVDSSSRLTKLLANPTTHSHLNRQTFTSLYKP-NQSRHFRTHYLPSSPSSPPVSRF 65

Query: 62  DRSRSWSSHRSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKM 121
           D S+ W S     EK  GFF+   +GN  +           + +LVE+R  F G QF K 
Sbjct: 66  DPSQLWRS-----EKIRGFFA-SALGNKAV----------KLGNLVESRVGFIGSQFPKK 125

Query: 122 RFQLKPGSDYQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTIS 181
            F+ +  S +Q RG   ++ W QGL+  DVVLGL+IAN GVF++WR+ +  FM  NF IS
Sbjct: 126 GFEFQRFSGFQRRG---WKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMIS 185

Query: 182 VENVRSGRIHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGS 241
           ++N +SGR+HT+ITSAFSH+DIGHIVSNMIGLYFFG +I R FGPQFLLKLYLAGA+GGS
Sbjct: 186 LDNFKSGRLHTLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGS 245

Query: 242 AFYLAHHLFQASSS-KGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIP 301
            FYL HH + A++S KG+  + +DP+R  GLGASGAVNAIMLLDIFL P++TLYLEFFIP
Sbjct: 246 VFYLIHHAYMAATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIP 305

Query: 302 VPAILLGIFLIGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           VPA+LLGIFLIGKD+LRI E +S ISGSAHLGGAAVAA+AWAR+R+GRF
Sbjct: 306 VPAMLLGIFLIGKDILRITEGNSNISGSAHLGGAAVAAIAWARIRKGRF 334

BLAST of Sed0013495 vs. ExPASy Swiss-Prot
Match: Q84WG3 (Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.6e-47
Identity = 115/284 (40.49%), Postives = 168/284 (59.15%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + + HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRI--ADPIFMSKNFTISVENVRSGR 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR+   D ++M KNF +S  +  +GR
Sbjct: 112 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGR 171

Query: 182 IHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHL 241
           IHT+ITS FSHV   HI+ NM+GL +FG  I R FGP++LLKLY AGA+GGS F+L+ H 
Sbjct: 172 IHTLITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHA 231

Query: 242 FQASSSKGRSLWGKDPARAQ--GLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
               S KG+ +  KD  +     LGA+G V AI LLD+ L+PK T Y    + VP +  G
Sbjct: 232 LSVISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAG 291

Query: 302 IFLIGKDMLRILE--NDSQISGSAHLGGAAVAALAWARVRRGRF 340
           I+ +G +++++LE  N++ ++    LGG  VA +AWAR+R+GRF
Sbjct: 292 IYSLGLNIIKMLEGKNNNTLTSLDQLGGVVVAVIAWARIRKGRF 320

BLAST of Sed0013495 vs. ExPASy Swiss-Prot
Match: B3H707 (Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.3e-39
Identity = 108/284 (38.03%), Postives = 158/284 (55.63%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + K HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 58  SRSAKVHGFFASKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 117

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRIH 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR++D     +++ +S  +  SG IH
Sbjct: 118 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIH 177

Query: 182 TMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLFQ 241
           T+ITS FSH+    I+ NMIG+ +FG  I R  GP +LLKLY AGA+GGS  +L++H   
Sbjct: 178 TLITSGFSHIGTSQIILNMIGISYFGSRIARTLGPLYLLKLYFAGALGGSVCFLSYHALL 237

Query: 242 ASSSKGRSLWGKDPAR----AQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
           A + KG  +  KD       +Q LGA G++ AI LLD+F++PK T Y    + V  ++  
Sbjct: 238 A-TLKGEGVVIKDHQSTAPISQLLGADGSMFAIALLDMFIYPKVTTYFALMLRV-HVMFR 297

Query: 302 IFLIGKDMLRILENDSQ--ISGSAHLGGAAVAALAWARVRRGRF 340
           I  +G ++L I E       S S  LGG  VAA+AWAR+++GRF
Sbjct: 298 IINLGVEILNIPEGGPNHIASSSGQLGGVVVAAMAWARIKKGRF 320

BLAST of Sed0013495 vs. ExPASy Swiss-Prot
Match: P53259 (Rhomboid protein 1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PCP1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.7e-08
Identity = 56/196 (28.57%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 140 DVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRIHTMITSAFSHVDIGHIVSN 199
           ++V  L+  NV VF LW++       + + +  ++  + +I ++I SAFSH +  H+  N
Sbjct: 144 NLVYALLGINVAVFGLWQLPKCWRFLQKYMLLQKDYVTSKI-SIIGSAFSHQEFWHLGMN 203

Query: 200 MIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLFQASSSKGRSLWGKDPAR-- 259
           M+ L+ FG ++  + G      LY+  AI GS F               SLW    AR  
Sbjct: 204 MLALWSFGTSLATMLGASNFFSLYMNSAIAGSLF---------------SLWYPKLARLA 263

Query: 260 --AQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL--IGKDMLRILENDS 319
                LGASGA+  ++    +LFP + + L F  PVP      FL  +  +         
Sbjct: 264 IVGPSLGASGALFGVLGCFSYLFPHAKILL-FVFPVPGGAWVAFLASVAWNAAGCALRWG 322

Query: 320 QISGSAHLGGAAVAAL 330
               +AHLGG+ +  L
Sbjct: 324 SFDYAAHLGGSMMGVL 322

BLAST of Sed0013495 vs. ExPASy Swiss-Prot
Match: Q2KHV4 (Presenilins-associated rhomboid-like protein, mitochondrial OS=Bos taurus OX=9913 GN=PARL PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-05
Identity = 58/205 (28.29%), Postives = 86/205 (41.95%), Query Frame = 0

Query: 126 RTRFQRWFQGLTTND-VVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI-HTM 185
           R    +W+  L+     V G+I ANV VF LWR+  P            N  S  +   M
Sbjct: 150 RKEINKWWNNLSDGQRTVTGIIAANVFVFCLWRV--PSLQRTMIRYFTSNPASKVLCSPM 209

Query: 186 ITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYL-AGAIGGSAFYLAHHLFQA 245
           + S FSH  + H+ +NM  L+ F  +I  + G +  + +YL AG I     Y+      A
Sbjct: 210 LLSTFSHFSLFHMAANMYVLWSFSSSIVNILGQEQFMAVYLSAGVISTFVSYVCK---VA 269

Query: 246 SSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL-- 305
           +   G S           LGASGA+  ++       P+  L +  F+P+     G  L  
Sbjct: 270 TGRYGPS-----------LGASGAIMTVLAAVCTKIPEGRLAI-IFLPMFTFTAGNALKA 329

Query: 306 -IGKDMLRILENDSQISGSAHLGGA 325
            I  D   ++        +AHLGGA
Sbjct: 330 IIAMDTAGMILGWKFFDHAAHLGGA 337

BLAST of Sed0013495 vs. ExPASy TrEMBL
Match: A0A6J1C609 (RHOMBOID-like protein 12, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111008595 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.7e-156
Identity = 282/339 (83.19%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNKLF  +LASR+SNS A+S PCK+C SFATLVRTHNQN LFGSF++RPLQD S SWSS 
Sbjct: 1   MNKLFSLKLASRISNSTATSRPCKNCTSFATLVRTHNQNHLFGSFISRPLQDHSHSWSSQ 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSFAEK HG FS  V+G  FLL+ SNTLFK S++SL+E RFLFTG QFSK+RFQLKPG D
Sbjct: 61  RSFAEKIHGLFSVSVVGKRFLLNSSNTLFKGSIRSLIERRFLFTGAQFSKLRFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           +QWRGR   + WFQ LT+NDVVLGLIIANV VFLLWRIADP+FMS+NFTISVEN+RSGR+
Sbjct: 121 FQWRGR---RSWFQRLTSNDVVLGLIIANVAVFLLWRIADPVFMSRNFTISVENIRSGRV 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV++GHIVSNMIGLYFFGMNIGRVFG +FLL+LYLAGAIGGSAFYL HHLF
Sbjct: 181 HTLITSAFSHVEVGHIVSNMIGLYFFGMNIGRVFGSEFLLRLYLAGAIGGSAFYLVHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK R  WGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSKDRPFWGKDPIRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 336

BLAST of Sed0013495 vs. ExPASy TrEMBL
Match: A0A6J1E6X7 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111431274 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.5e-155
Identity = 285/339 (84.07%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNK+F R+LASRLSNS+ +SH C+DCASFAT VRT NQN  +GSF+TRP++D SRSWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSF EK HGFFS PV+G  F+L+PSNTLFK S+KSLV  R LF G  FSK++FQLKPG D
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           ++W GR   + WFQ LTTNDVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HRWSGR---RAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV+IGHIVSNMIGLYFFGMNIGRVFGP+FLL LYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSS+ R  WGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sed0013495 vs. ExPASy TrEMBL
Match: A0A6J1KPT4 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495401 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 285/339 (84.07%), Postives = 306/339 (90.27%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNK F R+LASRLSNS+ +SH C+DCASFAT VRT NQN  +GSF+TRP++D SRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RS  EK HGFFS PV+G  F+L+PS+TLFK S+KSLVE R LF G  FSK++FQLKPG D
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVE-RSLFMGSPFSKLKFQLKPGFD 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           ++W GR   + WFQ LTTNDVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HRWPGR---RAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSHV+IGHIVSNMIGLYFFGMNIGRVFGP+FLL LYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK R  WGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL
Sbjct: 241 QASSSKSRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sed0013495 vs. ExPASy TrEMBL
Match: A0A0A0LTI7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 279/339 (82.30%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNKLF R+L SRLSNS+A+S  CKDCASF+TLVRTHNQN LFGSF+TRP+QD SRSWSSH
Sbjct: 1   MNKLFSRKLVSRLSNSYANSLDCKDCASFSTLVRTHNQNHLFGSFVTRPIQDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSFA+K HGFFS PV+G  F+++PSNT FK SMKS+VE+R LFTG  FSK++FQLKPG  
Sbjct: 61  RSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           +QW GR   + WFQ LT N+VVLGLIIANV VFLLWRIADPIFMSKNFTIS+ENVRSGRI
Sbjct: 121 HQWGGR---RSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSH+D GHIVSNM+GLYFFGMNIG VFG +FLLKLYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHIDTGHIVSNMVGLYFFGMNIGNVFGSEFLLKLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QA SSK RS WG+DP RA+GLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFL
Sbjct: 241 QALSSKSRSFWGEDPVRAKGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLR+LENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRMLENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sed0013495 vs. ExPASy TrEMBL
Match: A0A1S3BSX2 (RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 282/339 (83.19%), Postives = 305/339 (89.97%), Query Frame = 0

Query: 1   MNKLFVRQLASRLSNSFASSHPCKDCASFATLVRTHNQNRLFGSFLTRPLQDRSRSWSSH 60
           MNKLF R+LASRLSNS+A+S  CKDCASF+TL RTHNQN LFGSF+TRPLQD SRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  RSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSD 120
           RSF +K HGFFS PV+G   +++PSNT FK SMKS+VE+R LFTG  FSK++FQLKPG  
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 YQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRI 180
           +QW GR   + WFQ LT  DVVLGLIIANV VFLLWRIADPIFMSKNFTISVENVRSGRI
Sbjct: 121 HQWGGR---RSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRI 180

Query: 181 HTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLF 240
           HT+ITSAFSH+D GH++SNMIGLYFFGMNIG VFG +FLLKLYLAGAIGGSAFYLAHHLF
Sbjct: 181 HTLITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLAHHLF 240

Query: 241 QASSSKGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 300
           QASSSK RS WGKDP RAQGLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFL
Sbjct: 241 QASSSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFL 300

Query: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Sbjct: 301 IGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sed0013495 vs. TAIR 10
Match: AT1G18600.1 (RHOMBOID-like protein 12 )

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-90
Identity = 192/349 (55.01%), Postives = 245/349 (70.20%), Query Frame = 0

Query: 2   NKLFVRQLASRLSNSFA--SSHPCKDCASFATLVRTHNQNRLF------GSFLTRPLQ-- 61
           N+  V   +SRL+   A  ++H   +  +F +L +  NQ+R F       S  + P+   
Sbjct: 6   NRRVVVDSSSRLTKLLANPTTHSHLNRQTFTSLYKP-NQSRHFRTHYLPSSPSSPPVSRF 65

Query: 62  DRSRSWSSHRSFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKM 121
           D S+ W S     EK  GFF+   +GN  +           + +LVE+R  F G QF K 
Sbjct: 66  DPSQLWRS-----EKIRGFFA-SALGNKAV----------KLGNLVESRVGFIGSQFPKK 125

Query: 122 RFQLKPGSDYQWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTIS 181
            F+ +  S +Q RG   ++ W QGL+  DVVLGL+IAN GVF++WR+ +  FM  NF IS
Sbjct: 126 GFEFQRFSGFQRRG---WKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMIS 185

Query: 182 VENVRSGRIHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGS 241
           ++N +SGR+HT+ITSAFSH+DIGHIVSNMIGLYFFG +I R FGPQFLLKLYLAGA+GGS
Sbjct: 186 LDNFKSGRLHTLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGS 245

Query: 242 AFYLAHHLFQASSS-KGRSLWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIP 301
            FYL HH + A++S KG+  + +DP+R  GLGASGAVNAIMLLDIFL P++TLYLEFFIP
Sbjct: 246 VFYLIHHAYMAATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIP 305

Query: 302 VPAILLGIFLIGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 340
           VPA+LLGIFLIGKD+LRI E +S ISGSAHLGGAAVAA+AWAR+R+GRF
Sbjct: 306 VPAMLLGIFLIGKDILRITEGNSNISGSAHLGGAAVAAIAWARIRKGRF 334

BLAST of Sed0013495 vs. TAIR 10
Match: AT1G74130.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.6e-48
Identity = 115/284 (40.49%), Postives = 168/284 (59.15%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + + HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRI--ADPIFMSKNFTISVENVRSGR 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR+   D ++M KNF +S  +  +GR
Sbjct: 112 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGR 171

Query: 182 IHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHL 241
           IHT+ITS FSHV   HI+ NM+GL +FG  I R FGP++LLKLY AGA+GGS F+L+ H 
Sbjct: 172 IHTLITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHA 231

Query: 242 FQASSSKGRSLWGKDPARAQ--GLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
               S KG+ +  KD  +     LGA+G V AI LLD+ L+PK T Y    + VP +  G
Sbjct: 232 LSVISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAG 291

Query: 302 IFLIGKDMLRILE--NDSQISGSAHLGGAAVAALAWARVRRGRF 340
           I+ +G +++++LE  N++ ++    LGG  VA +AWAR+R+GRF
Sbjct: 292 IYSLGLNIIKMLEGKNNNTLTSLDQLGGVVVAVIAWARIRKGRF 320

BLAST of Sed0013495 vs. TAIR 10
Match: AT1G74140.5 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 165.2 bits (417), Expect = 8.9e-41
Identity = 108/284 (38.03%), Postives = 158/284 (55.63%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + K HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 58  SRSAKVHGFFASKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 117

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRIH 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR++D     +++ +S  +  SG IH
Sbjct: 118 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIH 177

Query: 182 TMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLFQ 241
           T+ITS FSH+    I+ NMIG+ +FG  I R  GP +LLKLY AGA+GGS  +L++H   
Sbjct: 178 TLITSGFSHIGTSQIILNMIGISYFGSRIARTLGPLYLLKLYFAGALGGSVCFLSYHALL 237

Query: 242 ASSSKGRSLWGKDPAR----AQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
           A + KG  +  KD       +Q LGA G++ AI LLD+F++PK T Y    + V  ++  
Sbjct: 238 A-TLKGEGVVIKDHQSTAPISQLLGADGSMFAIALLDMFIYPKVTTYFALMLRV-HVMFR 297

Query: 302 IFLIGKDMLRILENDSQ--ISGSAHLGGAAVAALAWARVRRGRF 340
           I  +G ++L I E       S S  LGG  VAA+AWAR+++GRF
Sbjct: 298 IINLGVEILNIPEGGPNHIASSSGQLGGVVVAAMAWARIKKGRF 320

BLAST of Sed0013495 vs. TAIR 10
Match: AT1G74130.2 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-40
Identity = 101/253 (39.92%), Postives = 147/253 (58.10%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + + HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRI--ADPIFMSKNFTISVENVRSGR 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR+   D ++M KNF +S  +  +GR
Sbjct: 112 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGR 171

Query: 182 IHTMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHL 241
           IHT+ITS FSHV   HI+ NM+GL +FG  I R FGP++LLKLY AGA+GGS F+L+ H 
Sbjct: 172 IHTLITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHA 231

Query: 242 FQASSSKGRSLWGKDPARAQ--GLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
               S KG+ +  KD  +     LGA+G V AI LLD+ L+PK T Y    + VP +  G
Sbjct: 232 LSVISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAG 289

Query: 302 IFLIGKDMLRILE 311
           I+ +G +++++LE
Sbjct: 292 IYSLGLNIIKMLE 289

BLAST of Sed0013495 vs. TAIR 10
Match: AT1G74140.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-40
Identity = 108/284 (38.03%), Postives = 157/284 (55.28%), Query Frame = 0

Query: 62  SFAEKFHGFFSRPVIGNGFLLDPSNTLFKRSMKSLVENRFLFTGGQFSKMRFQLKPGSDY 121
           S + K HGFF+  +          NT  K    +++E+R  F   +     F+    + +
Sbjct: 58  SRSAKVHGFFASKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 117

Query: 122 QWRGRTRFQRWFQGLTTNDVVLGLIIANVGVFLLWRIADPIFMSKNFTISVENVRSGRIH 181
           Q RG   ++ W  G   N VV GL+IAN  VF +WR++D     +++ +S  +  SG IH
Sbjct: 118 QKRG---WKSWING--ANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIH 177

Query: 182 TMITSAFSHVDIGHIVSNMIGLYFFGMNIGRVFGPQFLLKLYLAGAIGGSAFYLAHHLFQ 241
           T+ITS FSH+    I+ NMIG+ +FG  I R  GP +LLKLY AGA+GGS  +L++H   
Sbjct: 178 TLITSGFSHIGTSQIILNMIGISYFGSRIARTLGPLYLLKLYFAGALGGSVCFLSYHALL 237

Query: 242 ASSSKGRSLWGKDPAR----AQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLG 301
           A + KG  +  KD       +Q LGA G++ AI LLD+F++PK T Y    + V  I+  
Sbjct: 238 A-TLKGEGVVIKDHQSTAPISQLLGADGSMFAIALLDMFIYPKVTTYFALMLRVHRII-- 297

Query: 302 IFLIGKDMLRILENDSQ--ISGSAHLGGAAVAALAWARVRRGRF 340
              +G ++L I E       S S  LGG  VAA+AWAR+++GRF
Sbjct: 298 --NLGVEILNIPEGGPNHIASSSGQLGGVVVAAMAWARIKKGRF 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893963.11.4e-15786.43RHOMBOID-like protein 12, mitochondrial [Benincasa hispida][more]
XP_022137034.13.5e-15683.19RHOMBOID-like protein 12, mitochondrial [Momordica charantia][more]
XP_022923619.15.1e-15584.07RHOMBOID-like protein 12, mitochondrial [Cucurbita moschata][more]
KAG7019987.11.1e-15484.07RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_023001193.12.5e-15484.07RHOMBOID-like protein 12, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FZ811.7e-8955.01RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12... [more]
Q84WG33.6e-4740.49Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16... [more]
B3H7071.3e-3938.03Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17... [more]
P532595.7e-0828.57Rhomboid protein 1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
Q2KHV41.2e-0528.29Presenilins-associated rhomboid-like protein, mitochondrial OS=Bos taurus OX=991... [more]
Match NameE-valueIdentityDescription
A0A6J1C6091.7e-15683.19RHOMBOID-like protein 12, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1E6X72.5e-15584.07RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KPT41.2e-15484.07RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A0A0LTI72.7e-15482.30Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE... [more]
A0A1S3BSX22.7e-15483.19RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 ... [more]
Match NameE-valueIdentityDescription
AT1G18600.11.2e-9055.01RHOMBOID-like protein 12 [more]
AT1G74130.12.6e-4840.49Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.58.9e-4138.03Rhomboid-related intramembrane serine protease family protein [more]
AT1G74130.21.5e-4039.92Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.12.0e-4038.03Rhomboid-related intramembrane serine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 176..334
e-value: 6.2E-27
score: 94.4
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 136..337
e-value: 4.5E-34
score: 119.7
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 141..335
NoneNo IPR availablePANTHERPTHR43731RHOMBOID PROTEASEcoord: 8..336
NoneNo IPR availablePANTHERPTHR43731:SF22RHOMBOID-LIKE PROTEIN 12, MITOCHONDRIALcoord: 8..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0013495.1Sed0013495.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity