Sed0013473 (gene) Chayote v1

Overview
NameSed0013473
Typegene
OrganismSechium edule (Chayote v1)
DescriptionDelta(12)-fatty-acid desaturase
LocationLG12: 9003807 .. 9008048 (-)
RNA-Seq ExpressionSed0013473
SyntenySed0013473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAAATTCCAGAGAAAAAGGCTTCGTTGCCGGCGATCTGAAGGCGGCGAAAGCTTTCAGGGCGGAGATTGCGAGGTCACTCAAACTTCGATCCTCTTCTTATCTTTCAGGTATCATTTCCTTCACCATTCTGTTCATGTTTTTCTGTGGATTATTGATTCTGTTTCTCTTGATTTGTTATCGTTCAATCTGATGGACTTTTATTTAGTTTTTTTTTGTTGTTGTTTGGATTGTTGCTGATGATGTTTGTGGTAATGTATAGATCTGTGTTGAGATTTAGATTACTGTTCGCTAAAATGCTCTGTAACGGAGATTGCAGTGTCTCCCGTGCATGTAGTTCGATTTTAGGTTTTCGCTGAGATTTAGGACTGTGACGATTCAGGCTGTAGGTTTTAGCTTAATTTTTCGCTTTAGAAGCTCTGAAATCGCGTGGTTTCGATCTGGATATCTAGATTTGGCTCTTCCGTAATGGCTACGCTTTTGCAAATGCTGAGGATTTTTTTATTTGTGAGCCAATCGGAATCTCGTTTCCGTTTTCTTCTTTGTACAGATTTTCTCACCGGTTAATCGGTCTGTGCTATCTCATTTTTCAATTTCCGGGATTATTTTTTACATTAAAAGAATTTGTGGAAATATTTCTGGATTTTTTTTGTAATCGGGAAATCAAAGCGTGGGCAAGAATGGAGAGACAAATTACACTTGGATATTTTACAATTAAGTTCAGTTCTAGGTTTCAAAATCTGAGATCTTATTAAAGTAATGCCTAGAAAGTCACCAAAGAGGCTGGACCTTAGGGACTGAAATATTTTCCGGCTTTAGAGAGGTCCTCCTTGAGACCACACTGGAAGAAGTATATTTTTTTACCTAATTGTTTTTTTTTTCGTCTCCTGTAGTATTAAAGTATTAATGGCTCTTACACTTAATTAAGTTCTGATATTTTGTTGATTATTATTATTATTAATTATTGTTGTTATTTATGGTTCCGATTAGCAGTCTCGAGTCAATTAGGGTAGGCGCTATTCAGCATGTGTATTTTGTGTTCTAAGAAAGAAAAGAGAAAAATCCACCGTCCTACCTTTGCATTTGACCGTCTTTTGAACCGTGATTTGGTGAACTTATATTGTTGTGTTTTTTAATAAAAGCAATCTTCTTATAAAAGAAACATGGAAAATGTTGAATTCAAATAAAGAAAAATAACATAGAAAACAATCAATCAAACATACTTTCAGTAAAATTTTTATTTGATTAAAACATCCACACCGTTCCTTTTATGTACCAAACGTCTTGATCAACAAATTTGGGAGAATACGTTTCCCAAAAATTTAAATCGTTATTACACATATTTTGAACTCACGCCCTCTTGCCCTCTTGCCCTCCTACCCTCGTATATGTCTTATAAACTATCAGAACTCAATTAGCATGTCAAAGAGGGATTGAATATTCAAGATTTTAATTGATTATCAATCTATTACAGTCATGTACTTAAAGAAATAGTTTTCTATGTATTTCTTTACATCTTAATGTTGTGGGGTCAAAGAGCAAAACCGACATGATGCATAAGCTATAATTTTTCTTTGTATAGAATTTTATGTTTACCTGATAAAAATATGAAACAAATGCTTATATATAAACCAGAATGTGGGTTTCTTGTTAAAATTCAACTATGGGTGAATTACTGTTCATACGTTGAATGAATAGAAATAGAACCACCCATTGGCTGAACCTGAATATCATTTTAATTAAGTTTCATGATAAGCAGTTTTTCTTTTTTAAATGTTTGGTAGGGTCTTTTTTTTTTATGAAATATGTTTGGTAGGGTCTAGTAGTATGCAAAATATTCTAATTTACAGCCATTCGCCTGACATAATTGCGAAGTTGTGACAGCAACGTTATCGCTCTAGGCGTTCTTCGATTTAATTGAGGATTCCACTTGCACTTTTTAATTACATACTACATAGGACTTTGCCTGTTTCTATCTTATCTTTTCTCATACTGCAACAAGTAGGCGGCGAAGTTTTTTGCAAGTTTGTTTTCTTTGTACTGTGCCACCCCGTTCTTCTGGGCCTTTTTTTCAATTACGTTGAATTATTAGGCTACCATTGAATTCATGTATTTACTAATCTTTTTAGTAATGCTTGTGGGCCAGATTTTTTTTACTCTATTTGATGAATGCATCTTGCTAATACCTCTACTAGATATAAGTGGAAATCACTTTTCTTTTTTTTCTTTTTTTTTTTCTGAAATCAGAAATTACTTTTCTTAATTTAGAGAATTTATTAGGCGTTGTGGGGTGATATAGTGGTTAAAGAGAGACTTGGGATACAAAAAGTTATAGTTGTTTGCTTGAAAATTAATTTTCTGTGAATTTATTCTTATTCAAATGTTGTAAGATTAGATGGGTGAGAAAAGTAGTAATGCCCAATTTGACATTCACTGTGTTCGAAAAAAAAATCAGAGATTTGAATGCATGAATCTCTCGTCTCATTAATGCTGACTAGTGAAATTTTATGAAAATGATTGTTGGGCTCTTGTGACAAACAGTATTATGCTTGGTTGTTAGCTGTTAAATGAATCAATTTTATAATCTCATTTGTTTTGCTTGTTCCAGGGCAGTATCCTTGAAAAATGGGTGGTGGAGGTCGAATGTCATCTCGTTCGGACAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACTAAACCCCCATTCACCCTTGGTGAGCTCAAAAAGGCCATTCCTCCACATTGCTTTCAACGTTCGGTTCTTCGTTCATTTTCCTATGTCGTTTATGACCTTATTCTAGCCTCCATCTTCTATTACATTGCAACCACTTACTTCCAAAACCTTCCGAGCCTCTTGTTTTATCCTGCTTGGGCTTTATACTGGGCTGCTCAAGGTTGCGTCCTTACTGGAGTATGGGTCCTTGCTCACGAGTGTGGCCACCATGCCTTCAGTGATTACCAGTGGCTCGATGACACGGTCGGCCTCATCCTCCACTCTTTCCTCATGGTCCCTTACTTCTCTTGGAAATACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAAAAATCCGCTCTCAGCTGGTTCTCAAAATACCTCAACAACCCACCTGGCCGAGTCCTCACCCTCGCTGTCACCCTCACCTTAGGGTGGCCTCTCTACTTAGCCTTCAACATCTCTGGCCGACCTTACAATCGTTTTGCCTGCCACTTTGACCCATATGGCCCCATCTACTCTGACCGTGAGCGGTTGCAGATATTCATCTCCGATGTAGGGTTGCTGACTGTCACATATGTACTCTACCGCCTTGCTCTAGCCAAAGGAATCGCCTGGGTGGTCTGTGTTTACGGAGTGCCATTACTGATTGTGAACGGGTTCCTTGTCCTAATCACATTCCTTCAGCACACTCACCCAGCATTGCCTCATTACGATTCCTCCGAATGGGACTGGATGAGAGGCGCATTGGCGACAGTGGACCGAGACTATGGGATATTGAACAAGGTGTTCCACAACATCACAGACACTCACGTGGCCCATCATTTGGTATCGACAATGCCGCATTACCATGCCATGGAGGCAACCAATGCAATTAAGCCAATTTTGGGACAGTACTACCACTTCGACGGGACTCCATTTGTGAAGGCAATGTGGAGAGAGGCAAAAGAGTGCATATATGTGGCGCCTGATGAAGGCGAGTCTAAAGGCGTTTTCTGGTATAGGAACAAGTTGTAATCTGTTTGTGGAGGATGGGCAGGTGATTTCTTTTCAGCCATTTTCTCTATCCAGGTTCTGCTACTCTACTCTCTAGATAGAACTGTTTGCTTTTTACCTTTATTATGGACCTTAGTTTCTATGATGGGTTAGTTTACTACTGCTGTATCAAAATGCATAAACGGTGTGTTAGGTCGGTCTAGTTTTCTTCGTTGTTTTGCTTCCTAATATGGCATCCATTCTAGATTACGAGCAACTATTCATTATGTGGAATTGGATGCGTGACGATGGATGTTCCCTTCAAATATGAATGCATGCGGTTAAACTGTTATGTAACAATTTGGATAAGTTAGGGGAAGATGACCTCTGAGATGCTATGATGAACAATGTGGAATAAATTTTGTAAGATTCGAAAAATGTCGTTTGTTAGGTTATATGTTTGATTTTTTCATTTTTGAAATTTGAACTTATATTTACTTTAACTCTCTT

mRNA sequence

AAAGAAAAATTCCAGAGAAAAAGGCTTCGTTGCCGGCGATCTGAAGGCGGCGAAAGCTTTCAGGGCGGAGATTGCGAGGTCACTCAAACTTCGATCCTCTTCTTATCTTTCAGGGCAGTATCCTTGAAAAATGGGTGGTGGAGGTCGAATGTCATCTCGTTCGGACAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACTAAACCCCCATTCACCCTTGGTGAGCTCAAAAAGGCCATTCCTCCACATTGCTTTCAACGTTCGGTTCTTCGTTCATTTTCCTATGTCGTTTATGACCTTATTCTAGCCTCCATCTTCTATTACATTGCAACCACTTACTTCCAAAACCTTCCGAGCCTCTTGTTTTATCCTGCTTGGGCTTTATACTGGGCTGCTCAAGGTTGCGTCCTTACTGGAGTATGGGTCCTTGCTCACGAGTGTGGCCACCATGCCTTCAGTGATTACCAGTGGCTCGATGACACGGTCGGCCTCATCCTCCACTCTTTCCTCATGGTCCCTTACTTCTCTTGGAAATACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAAAAATCCGCTCTCAGCTGGTTCTCAAAATACCTCAACAACCCACCTGGCCGAGTCCTCACCCTCGCTGTCACCCTCACCTTAGGGTGGCCTCTCTACTTAGCCTTCAACATCTCTGGCCGACCTTACAATCGTTTTGCCTGCCACTTTGACCCATATGGCCCCATCTACTCTGACCGTGAGCGGTTGCAGATATTCATCTCCGATGTAGGGTTGCTGACTGTCACATATGTACTCTACCGCCTTGCTCTAGCCAAAGGAATCGCCTGGGTGGTCTGTGTTTACGGAGTGCCATTACTGATTGTGAACGGGTTCCTTGTCCTAATCACATTCCTTCAGCACACTCACCCAGCATTGCCTCATTACGATTCCTCCGAATGGGACTGGATGAGAGGCGCATTGGCGACAGTGGACCGAGACTATGGGATATTGAACAAGGTGTTCCACAACATCACAGACACTCACGTGGCCCATCATTTGGTATCGACAATGCCGCATTACCATGCCATGGAGGCAACCAATGCAATTAAGCCAATTTTGGGACAGTACTACCACTTCGACGGGACTCCATTTGTGAAGGCAATGTGGAGAGAGGCAAAAGAGTGCATATATGTGGCGCCTGATGAAGGCGAGTCTAAAGGCGTTTTCTGGTATAGGAACAAGTTGTAATCTGTTTGTGGAGGATGGGCAGGTGATTTCTTTTCAGCCATTTTCTCTATCCAGGTTCTGCTACTCTACTCTCTAGATAGAACTGTTTGCTTTTTACCTTTATTATGGACCTTAGTTTCTATGATGGGTTAGTTTACTACTGCTGTATCAAAATGCATAAACGGTGTGTTAGGTCGGTCTAGTTTTCTTCGTTGTTTTGCTTCCTAATATGGCATCCATTCTAGATTACGAGCAACTATTCATTATGTGGAATTGGATGCGTGACGATGGATGTTCCCTTCAAATATGAATGCATGCGGTTAAACTGTTATGTAACAATTTGGATAAGTTAGGGGAAGATGACCTCTGAGATGCTATGATGAACAATGTGGAATAAATTTTGTAAGATTCGAAAAATGTCGTTTGTTAGGTTATATGTTTGATTTTTTCATTTTTGAAATTTGAACTTATATTTACTTTAACTCTCTT

Coding sequence (CDS)

ATGGGTGGTGGAGGTCGAATGTCATCTCGTTCGGACAAGAAGTCAGATTCTGACCACTTGAAGCGAGTTCCATCCACTAAACCCCCATTCACCCTTGGTGAGCTCAAAAAGGCCATTCCTCCACATTGCTTTCAACGTTCGGTTCTTCGTTCATTTTCCTATGTCGTTTATGACCTTATTCTAGCCTCCATCTTCTATTACATTGCAACCACTTACTTCCAAAACCTTCCGAGCCTCTTGTTTTATCCTGCTTGGGCTTTATACTGGGCTGCTCAAGGTTGCGTCCTTACTGGAGTATGGGTCCTTGCTCACGAGTGTGGCCACCATGCCTTCAGTGATTACCAGTGGCTCGATGACACGGTCGGCCTCATCCTCCACTCTTTCCTCATGGTCCCTTACTTCTCTTGGAAATACAGCCACCGCAGACACCATTCCAACACCGGTTCTCTCGAGCGAGATGAAGTCTTTGTCCCTAAACAAAAATCCGCTCTCAGCTGGTTCTCAAAATACCTCAACAACCCACCTGGCCGAGTCCTCACCCTCGCTGTCACCCTCACCTTAGGGTGGCCTCTCTACTTAGCCTTCAACATCTCTGGCCGACCTTACAATCGTTTTGCCTGCCACTTTGACCCATATGGCCCCATCTACTCTGACCGTGAGCGGTTGCAGATATTCATCTCCGATGTAGGGTTGCTGACTGTCACATATGTACTCTACCGCCTTGCTCTAGCCAAAGGAATCGCCTGGGTGGTCTGTGTTTACGGAGTGCCATTACTGATTGTGAACGGGTTCCTTGTCCTAATCACATTCCTTCAGCACACTCACCCAGCATTGCCTCATTACGATTCCTCCGAATGGGACTGGATGAGAGGCGCATTGGCGACAGTGGACCGAGACTATGGGATATTGAACAAGGTGTTCCACAACATCACAGACACTCACGTGGCCCATCATTTGGTATCGACAATGCCGCATTACCATGCCATGGAGGCAACCAATGCAATTAAGCCAATTTTGGGACAGTACTACCACTTCGACGGGACTCCATTTGTGAAGGCAATGTGGAGAGAGGCAAAAGAGTGCATATATGTGGCGCCTGATGAAGGCGAGTCTAAAGGCGTTTTCTGGTATAGGAACAAGTTGTAA

Protein sequence

MGGGGRMSSRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVLTLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAKECIYVAPDEGESKGVFWYRNKL
Homology
BLAST of Sed0013473 vs. NCBI nr
Match: XP_004136572.1 (delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical protein Csa_001023 [Cucumis sativus])

HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 347/382 (90.84%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+S Y+NNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLLTVTY LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEGE KGV+WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382

BLAST of Sed0013473 vs. NCBI nr
Match: XP_038903906.1 (delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida])

HSP 1 Score: 741.5 bits (1913), Expect = 3.6e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLPSLLFYPAWA+YWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWAVYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPLGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
            L + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL + Y LY
Sbjct: 181 VLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAIAYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEG+ +GVFWY+NKL
Sbjct: 361 ECIYVEPDEGDKRGVFWYKNKL 382

BLAST of Sed0013473 vs. NCBI nr
Match: XP_022934859.1 (delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata])

HSP 1 Score: 741.1 bits (1912), Expect = 4.7e-210
Identity = 349/382 (91.36%), Postives = 363/382 (95.03%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
           ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382

BLAST of Sed0013473 vs. NCBI nr
Match: XP_008443062.2 (PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa] >TYK25404.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 740.7 bits (1911), Expect = 6.2e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Sed0013473 vs. NCBI nr
Match: KAG6580810.1 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-209
Identity = 348/382 (91.10%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
           ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382

BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 3.7e-189
Identity = 307/382 (80.37%), Postives = 340/382 (89.01%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMS     KK +S+ LKRVP +KPPFTLG+LKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            +A IFYYIAT YF  LP  L Y AW +YWA QGCVLTGVWV+AHECGHHAFSDYQ LDD
Sbjct: 61  TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
            VGL+LHS L+VPYFSWK+SHRRHHSNT SLERDEVFVPK+KS++ WFSKYLNNPPGR+ 
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL +TLTLGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRER +I+ISD G+L VT+ LY
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RLA AKG+AWV+CVYGVPLLIVN FLV+IT+LQHTHP++PHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG+YY FDGTPF KAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360

Query: 361 ECIYVAPDEG-ESKGVFWYRNK 381
           ECIYV  D+G ESKGV+WY  K
Sbjct: 361 ECIYVEADDGDESKGVYWYNKK 382

BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.1e-188
Identity = 309/383 (80.68%), Postives = 338/383 (88.25%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGR     +++KS+ D LKRVP  KP F+L ++KKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            +A   YY+AT YF  LP  L +   A+YWA QGC+LTGVWV+AHECGHHAFSDYQ LDD
Sbjct: 61  TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
            VGLILHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+SKYLNNPPGRVL
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TLAVTLTLGWPLYLA N+SGRPY+RFACH+DPYGPIYSDRERLQI+ISD G+L V Y L+
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLF 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RLA+AKG+AWVVCVYGVPLL+VNGFLVLITFLQHTHPALPHY SSEWDW+RGALATVDRD
Sbjct: 241 RLAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG+YY FD TPFVKAMWREA+
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAR 360

Query: 361 ECIYVAPDEG-ESKGVFWYRNKL 382
           ECIYV PD+  ESKGVFWY NKL
Sbjct: 361 ECIYVEPDQSTESKGVFWYNNKL 383

BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 651.7 bits (1680), Expect = 5.0e-186
Identity = 300/388 (77.32%), Postives = 335/388 (86.34%), Query Frame = 0

Query: 1   MGGGGRMS------SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSY 60
           MG GGRM+         D+KS    ++RVPS KPPFTL E+KKAIPPHCF+RS+L+SFSY
Sbjct: 1   MGAGGRMTVPNKWEGEGDEKSQKP-VQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSY 60

Query: 61  VVYDLILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDY 120
           V+YDL L +IFYY+ATTY   LP  L Y AW +YWA QGCVLTGVWV+AHECGHHAFSDY
Sbjct: 61  VLYDLTLVAIFYYVATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDY 120

Query: 121 QWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNP 180
           QW+DD VGL+LHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPK KS + WFSKYLNNP
Sbjct: 121 QWVDDCVGLVLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNP 180

Query: 181 PGRVLTLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTV 240
           PGRV+TL VTLTLGWPLYLA N+SG PY+RFACHFDPYGPIY+DRERLQI+ISDVG++  
Sbjct: 181 PGRVMTLIVTLTLGWPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAA 240

Query: 241 TYVLYRLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALA 300
           TY LY++A A+G+AW+VCVYGVPLLIVN FLV IT+LQHTHPALPHYDSSEWDW+RGALA
Sbjct: 241 TYTLYKIAAARGLAWLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALA 300

Query: 301 TVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAM 360
           T DRDYGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG YY FDGTP  KAM
Sbjct: 301 TADRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAM 360

Query: 361 WREAKECIYVAPDEG-ESKGVFWYRNKL 382
           WREA+EC+YV PD+G  SKGVFWY+  L
Sbjct: 361 WREARECLYVEPDDGANSKGVFWYKKNL 387

BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match: Q39287 (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-181
Identity = 299/384 (77.86%), Postives = 330/384 (85.94%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRM  S S KKS++D LKRVP   PPFT+GELKKAIPPHCF+RS+ RSFSY+++D+
Sbjct: 1   MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
           I+AS FYY+ATTYF  LP  L Y AW LYWA QG VLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS + W+ KYLNNP GR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPY-NRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVL 240
            L V  TLGWPLY AFN+SGRPY   FACHF P  PIY+DRERLQI++SD G+L V Y L
Sbjct: 181 MLTVQFTLGWPLYWAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240

Query: 241 YRLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDR 300
           YR A A+G+A +VC+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREA 360
           DYGILNKVFHNITDTHVAHHL STMPHYHAME T AIKPILG YY FDGTP+VKAMWREA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREA 360

Query: 361 KECIYVAPD-EGESKGVFWYRNKL 382
           KECIYV PD +GE KGVFWY NKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384

BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match: P46313 (Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-179
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRM    S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
           I+AS FYY+AT YF  LP  L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
            L V   LGWPLYLAFN+SGRPY+ FACHF P  PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+  AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
           ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

BLAST of Sed0013473 vs. ExPASy TrEMBL
Match: A0A0A0LF25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 7.9e-211
Identity = 347/382 (90.84%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+S Y+NNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLLTVTY LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEGE KGV+WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382

BLAST of Sed0013473 vs. ExPASy TrEMBL
Match: A0A6J1F3S1 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC111441899 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.3e-210
Identity = 349/382 (91.36%), Postives = 363/382 (95.03%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
           ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382

BLAST of Sed0013473 vs. ExPASy TrEMBL
Match: A0A5A7TLK3 (Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005460 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.0e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Sed0013473 vs. ExPASy TrEMBL
Match: A0A1S3B762 (delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.0e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
           ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Sed0013473 vs. ExPASy TrEMBL
Match: A0A6J1J0W0 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111481665 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 1.9e-209
Identity = 348/382 (91.10%), Postives = 361/382 (94.50%), Query Frame = 0

Query: 1   MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRMSS S  KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
            LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
           TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
           ECIYV PD GE +KGVFWY NK
Sbjct: 361 ECIYVEPDAGEKTKGVFWYNNK 382

BLAST of Sed0013473 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-181
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRM    S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
           I+AS FYY+AT YF  LP  L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
            L V   LGWPLYLAFN+SGRPY+ FACHF P  PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+  AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
           ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

BLAST of Sed0013473 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-181
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0

Query: 1   MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MG GGRM    S KKS++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
           I+AS FYY+AT YF  LP  L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
            L V   LGWPLYLAFN+SGRPY+ FACHF P  PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
           R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+  AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
           ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

BLAST of Sed0013473 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 237.7 bits (605), Expect = 1.6e-62
Identity = 135/324 (41.67%), Postives = 183/324 (56.48%), Query Frame = 0

Query: 24  PSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQNLPSLLFYP 83
           PS +PPF +G+++ AIP HC+ +S LRS SYVV D+I  +    IA  Y  +      + 
Sbjct: 28  PSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAAL-AIAAVYVDS------WF 87

Query: 84  AWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 143
            W LYWAAQG +   ++VL H+CGH +FSD   L+  VG ILHSF++VPY  W+ SHR H
Sbjct: 88  LWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTH 147

Query: 144 HSNTGSLERDEVFVPKQKSALSWFSKYLNNPPG-RVLTLAVTL-TLGWPLYLAFNISGRP 203
           H N G +E DE +VP  +        Y   P   R+L   V L  L +PLYL +   G+ 
Sbjct: 148 HQNHGHVENDESWVPLPERV------YKKLPHSTRMLRYTVPLPMLAYPLYLCYRSPGKE 207

Query: 204 YNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCVYGVPLLIV 263
                 HF+PY  +++  ER  I  S      +   L  L+   G   V+ VYGVP +I 
Sbjct: 208 ----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVPYIIF 267

Query: 264 NGFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNITDTHVAHHL 323
             +L  +T+L H      LP Y   EW ++RG L T+DRDYGI N + H+I  THV HHL
Sbjct: 268 VMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDI-GTHVIHHL 327

Query: 324 VSTMPHYHAMEATNAIKPILGQYY 344
              +PHYH ++AT A K +LG+YY
Sbjct: 328 FPQIPHYHLVDATKAAKHVLGRYY 333

BLAST of Sed0013473 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 212.6 bits (540), Expect = 5.5e-55
Identity = 126/356 (35.39%), Postives = 185/356 (51.97%), Query Frame = 0

Query: 24  PSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQNLPSLLFYP 83
           P   PPF L +++ AIP HC+ ++  +S SYVV D  +A +F   A   + N      + 
Sbjct: 90  PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRD--VAIVFALAAGAAYLN-----NWI 149

Query: 84  AWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 143
            W LYW AQG +   ++VL H+CGH +FS+   L+  VG +LHS ++VPY  W+ SHR H
Sbjct: 150 VWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTH 209

Query: 144 HSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVLTLAVTLT-LGWPLYLAFNISGRPY 203
           H N G +E DE + P  +   +   K     P R     + L  L +P YL     G+  
Sbjct: 210 HQNHGHVENDESWHPMSEKIYNTLDK-----PTRFFRFTLPLVMLAYPFYLWARSPGKK- 269

Query: 204 NRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCVYGVPLLIVN 263
                H+ P   ++  +ER  +  S      +  +L  L    G   ++ +YG+P  I  
Sbjct: 270 ---GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINV 329

Query: 264 GFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNITDTHVAHHLV 323
            +L  +T+L H      LP Y   EW ++RG L T+DRDYG++N + H+I  THV HHL 
Sbjct: 330 MWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHHLF 389

Query: 324 STMPHYHAMEATNAIKPILGQYYHFDGTP------FVKAMWREAKECIYVAPDEGE 371
             +PHYH +EAT A KP+LG+YY             ++ + +  KE  YV+ DEGE
Sbjct: 390 PQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS-DEGE 427

BLAST of Sed0013473 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 204.1 bits (518), Expect = 1.9e-52
Identity = 118/332 (35.54%), Postives = 172/332 (51.81%), Query Frame = 0

Query: 15  SDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQ 74
           S+ D  +  P   PPF L +++ AIP HC+ ++   S SYVV D+ +      +A  YF 
Sbjct: 74  SEEDTERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVA-AYFN 133

Query: 75  NLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYF 134
           N      +  W LYW AQG +   ++VL H+CGH +FS+   L+   G +LHS ++VPY 
Sbjct: 134 N------WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYH 193

Query: 135 SWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVLTLAVTLTLGWP-LYL 194
            W+ SHR HH N G +E DE + P  +S      K          T     TL +P L  
Sbjct: 194 GWRISHRTHHQNHGHVENDESWHPLPESIYKNLEK---------TTQMFRFTLPFPMLAY 253

Query: 195 AFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCV 254
            F +  R   +   H+ P   ++  +E+  +  S      +  +L  L    G   ++ +
Sbjct: 254 PFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKL 313

Query: 255 YGVPLLIVNGFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNIT 314
           YG+P  I   +L  +T+L H      LP Y   EW ++RG L T+DRDYG +N + H+I 
Sbjct: 314 YGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI- 373

Query: 315 DTHVAHHLVSTMPHYHAMEATNAIKPILGQYY 344
            THV HHL   +PHYH +EAT A KP+LG+YY
Sbjct: 374 GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYY 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004136572.11.6e-21090.84delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical ... [more]
XP_038903906.13.6e-21090.58delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida][more]
XP_022934859.14.7e-21091.36delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata][more]
XP_008443062.26.2e-21090.58PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 del... [more]
KAG6580810.11.8e-20991.10Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbi... [more]
Match NameE-valueIdentityDescription
Q8GZC33.7e-18980.37Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV... [more]
P486313.1e-18880.68Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX... [more]
Q84VT25.0e-18677.32Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2[more]
Q392871.3e-18177.86Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 ... [more]
P463131.2e-17975.98Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LF257.9e-21190.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1[more]
A0A6J1F3S12.3e-21091.36delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5A7TLK33.0e-21090.58Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B7623.0e-21090.58delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=... [more]
A0A6J1J0W01.9e-20991.10delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G12120.18.4e-18175.98fatty acid desaturase 2 [more]
AT3G12120.28.4e-18175.98fatty acid desaturase 2 [more]
AT2G29980.11.6e-6241.67fatty acid desaturase 3 [more]
AT3G11170.15.5e-5535.39fatty acid desaturase 7 [more]
AT5G05580.11.9e-5235.54fatty acid desaturase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 83..344
e-value: 6.9E-30
score: 104.7
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 21..61
e-value: 2.4E-8
score: 34.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 1..381
NoneNo IPR availablePANTHERPTHR32100:SF40OLEATE HYDROXYLASE FAH12coord: 1..381
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 46..328
e-value: 2.49976E-77
score: 235.968

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0013473.1Sed0013473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045485 omega-6 fatty acid desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water