Homology
BLAST of Sed0013473 vs. NCBI nr
Match:
XP_004136572.1 (delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical protein Csa_001023 [Cucumis sativus])
HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 347/382 (90.84%), Postives = 362/382 (94.76%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+S Y+NNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLLTVTY LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEGE KGV+WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382
BLAST of Sed0013473 vs. NCBI nr
Match:
XP_038903906.1 (delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida])
HSP 1 Score: 741.5 bits (1913), Expect = 3.6e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLPSLLFYPAWA+YWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPSLLFYPAWAVYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPLGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
L + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL + Y LY
Sbjct: 181 VLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAIAYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEG+ +GVFWY+NKL
Sbjct: 361 ECIYVEPDEGDKRGVFWYKNKL 382
BLAST of Sed0013473 vs. NCBI nr
Match:
XP_022934859.1 (delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata])
HSP 1 Score: 741.1 bits (1912), Expect = 4.7e-210
Identity = 349/382 (91.36%), Postives = 363/382 (95.03%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360
Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382
BLAST of Sed0013473 vs. NCBI nr
Match:
XP_008443062.2 (PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa] >TYK25404.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa])
HSP 1 Score: 740.7 bits (1911), Expect = 6.2e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382
BLAST of Sed0013473 vs. NCBI nr
Match:
KAG6580810.1 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-209
Identity = 348/382 (91.10%), Postives = 362/382 (94.76%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360
Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382
BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match:
Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)
HSP 1 Score: 662.1 bits (1707), Expect = 3.7e-189
Identity = 307/382 (80.37%), Postives = 340/382 (89.01%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMS KK +S+ LKRVP +KPPFTLG+LKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
+A IFYYIAT YF LP L Y AW +YWA QGCVLTGVWV+AHECGHHAFSDYQ LDD
Sbjct: 61 TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
VGL+LHS L+VPYFSWK+SHRRHHSNT SLERDEVFVPK+KS++ WFSKYLNNPPGR+
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL +TLTLGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRER +I+ISD G+L VT+ LY
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RLA AKG+AWV+CVYGVPLLIVN FLV+IT+LQHTHP++PHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG+YY FDGTPF KAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360
Query: 361 ECIYVAPDEG-ESKGVFWYRNK 381
ECIYV D+G ESKGV+WY K
Sbjct: 361 ECIYVEADDGDESKGVYWYNKK 382
BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match:
P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)
HSP 1 Score: 659.1 bits (1699), Expect = 3.1e-188
Identity = 309/383 (80.68%), Postives = 338/383 (88.25%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGR +++KS+ D LKRVP KP F+L ++KKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
+A YY+AT YF LP L + A+YWA QGC+LTGVWV+AHECGHHAFSDYQ LDD
Sbjct: 61 TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
VGLILHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+SKYLNNPPGRVL
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TLAVTLTLGWPLYLA N+SGRPY+RFACH+DPYGPIYSDRERLQI+ISD G+L V Y L+
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLF 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RLA+AKG+AWVVCVYGVPLL+VNGFLVLITFLQHTHPALPHY SSEWDW+RGALATVDRD
Sbjct: 241 RLAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG+YY FD TPFVKAMWREA+
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAR 360
Query: 361 ECIYVAPDEG-ESKGVFWYRNKL 382
ECIYV PD+ ESKGVFWY NKL
Sbjct: 361 ECIYVEPDQSTESKGVFWYNNKL 383
BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match:
Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)
HSP 1 Score: 651.7 bits (1680), Expect = 5.0e-186
Identity = 300/388 (77.32%), Postives = 335/388 (86.34%), Query Frame = 0
Query: 1 MGGGGRMS------SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSY 60
MG GGRM+ D+KS ++RVPS KPPFTL E+KKAIPPHCF+RS+L+SFSY
Sbjct: 1 MGAGGRMTVPNKWEGEGDEKSQKP-VQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSY 60
Query: 61 VVYDLILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDY 120
V+YDL L +IFYY+ATTY LP L Y AW +YWA QGCVLTGVWV+AHECGHHAFSDY
Sbjct: 61 VLYDLTLVAIFYYVATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDY 120
Query: 121 QWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNP 180
QW+DD VGL+LHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPK KS + WFSKYLNNP
Sbjct: 121 QWVDDCVGLVLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNP 180
Query: 181 PGRVLTLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTV 240
PGRV+TL VTLTLGWPLYLA N+SG PY+RFACHFDPYGPIY+DRERLQI+ISDVG++
Sbjct: 181 PGRVMTLIVTLTLGWPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAA 240
Query: 241 TYVLYRLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALA 300
TY LY++A A+G+AW+VCVYGVPLLIVN FLV IT+LQHTHPALPHYDSSEWDW+RGALA
Sbjct: 241 TYTLYKIAAARGLAWLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALA 300
Query: 301 TVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAM 360
T DRDYGILNKVFHNITDTHVAHHL STMPHYHAMEAT AIKPILG YY FDGTP KAM
Sbjct: 301 TADRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAM 360
Query: 361 WREAKECIYVAPDEG-ESKGVFWYRNKL 382
WREA+EC+YV PD+G SKGVFWY+ L
Sbjct: 361 WREARECLYVEPDDGANSKGVFWYKKNL 387
BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match:
Q39287 (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 PE=2 SV=1)
HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-181
Identity = 299/384 (77.86%), Postives = 330/384 (85.94%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRM S S KKS++D LKRVP PPFT+GELKKAIPPHCF+RS+ RSFSY+++D+
Sbjct: 1 MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
I+AS FYY+ATTYF LP L Y AW LYWA QG VLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS + W+ KYLNNP GR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPY-NRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVL 240
L V TLGWPLY AFN+SGRPY FACHF P PIY+DRERLQI++SD G+L V Y L
Sbjct: 181 MLTVQFTLGWPLYWAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240
Query: 241 YRLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDR 300
YR A A+G+A +VC+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300
Query: 301 DYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREA 360
DYGILNKVFHNITDTHVAHHL STMPHYHAME T AIKPILG YY FDGTP+VKAMWREA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREA 360
Query: 361 KECIYVAPD-EGESKGVFWYRNKL 382
KECIYV PD +GE KGVFWY NKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384
BLAST of Sed0013473 vs. ExPASy Swiss-Prot
Match:
P46313 (Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=1)
HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-179
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRM S KKS++D KRVP KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
I+AS FYY+AT YF LP L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
L V LGWPLYLAFN+SGRPY+ FACHF P PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+ AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360
Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383
BLAST of Sed0013473 vs. ExPASy TrEMBL
Match:
A0A0A0LF25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1)
HSP 1 Score: 742.7 bits (1916), Expect = 7.9e-211
Identity = 347/382 (90.84%), Postives = 362/382 (94.76%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS + W+S Y+NNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLLTVTY LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEGE KGV+WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382
BLAST of Sed0013473 vs. ExPASy TrEMBL
Match:
A0A6J1F3S1 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC111441899 PE=3 SV=1)
HSP 1 Score: 741.1 bits (1912), Expect = 2.3e-210
Identity = 349/382 (91.36%), Postives = 363/382 (95.03%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360
Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
ECIYV PDEGE +KGVFWY NK
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNK 382
BLAST of Sed0013473 vs. ExPASy TrEMBL
Match:
A0A5A7TLK3 (Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005460 PE=3 SV=1)
HSP 1 Score: 740.7 bits (1911), Expect = 3.0e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382
BLAST of Sed0013473 vs. ExPASy TrEMBL
Match:
A0A1S3B762 (delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=3 SV=1)
HSP 1 Score: 740.7 bits (1911), Expect = 3.0e-210
Identity = 346/382 (90.58%), Postives = 360/382 (94.24%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL + L LGWPLYLAFN+SGRPY+RFACH+DPYGPIYSDRERLQIFISD GLL V Y LY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG AWVVCVYGVPLLIVNGFLVLIT+LQHTH ALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILGQYYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
Query: 361 ECIYVAPDEGESKGVFWYRNKL 382
ECIYV PDEGE KG++WY+NKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382
BLAST of Sed0013473 vs. ExPASy TrEMBL
Match:
A0A6J1J0W0 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111481665 PE=3 SV=1)
HSP 1 Score: 738.0 bits (1904), Expect = 1.9e-209
Identity = 348/382 (91.10%), Postives = 361/382 (94.50%), Query Frame = 0
Query: 1 MGGGGRMSSRSD-KKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRMSS S KKSDSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1 MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
LASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD
Sbjct: 61 TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSAL W+S YLNNP GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
TL +TL LGWPLYLAFN+SGRPY+RFACH+DPYGPIY+DRERLQIFISD GLL V+Y LY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
RL LAKG+AWVVCVYGVPLLIVNGFLVLIT+LQHTHPALPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHLVSTMPHYHAMEAT AIKPILG+YYHFDGTPFVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360
Query: 361 ECIYVAPDEGE-SKGVFWYRNK 381
ECIYV PD GE +KGVFWY NK
Sbjct: 361 ECIYVEPDAGEKTKGVFWYNNK 382
BLAST of Sed0013473 vs. TAIR 10
Match:
AT3G12120.1 (fatty acid desaturase 2 )
HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-181
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRM S KKS++D KRVP KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
I+AS FYY+AT YF LP L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
L V LGWPLYLAFN+SGRPY+ FACHF P PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+ AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360
Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383
BLAST of Sed0013473 vs. TAIR 10
Match:
AT3G12120.2 (fatty acid desaturase 2 )
HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-181
Identity = 291/383 (75.98%), Postives = 330/383 (86.16%), Query Frame = 0
Query: 1 MGGGGRMS-SRSDKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
MG GGRM S KKS++D KRVP KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 61 ILASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120
I+AS FYY+AT YF LP L Y AW LYWA QGCVLTG+WV+AHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVL 180
TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSA+ W+ KYLNNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180
Query: 181 TLAVTLTLGWPLYLAFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLY 240
L V LGWPLYLAFN+SGRPY+ FACHF P PIY+DRERLQI++SD G+L V + LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240
Query: 241 RLALAKGIAWVVCVYGVPLLIVNGFLVLITFLQHTHPALPHYDSSEWDWMRGALATVDRD 300
R A A+G+A ++C+YGVPLLIVN FLVLIT+LQHTHP+LPHYDSSEWDW+RGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300
Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATNAIKPILGQYYHFDGTPFVKAMWREAK 360
YGILNKVFHNITDTHVAHHL STMPHY+AMEAT AIKPILG YY FDGTP+ AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360
Query: 361 ECIYVAPD-EGESKGVFWYRNKL 382
ECIYV PD EG+ KGV+WY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383
BLAST of Sed0013473 vs. TAIR 10
Match:
AT2G29980.1 (fatty acid desaturase 3 )
HSP 1 Score: 237.7 bits (605), Expect = 1.6e-62
Identity = 135/324 (41.67%), Postives = 183/324 (56.48%), Query Frame = 0
Query: 24 PSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQNLPSLLFYP 83
PS +PPF +G+++ AIP HC+ +S LRS SYVV D+I + IA Y + +
Sbjct: 28 PSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAAL-AIAAVYVDS------WF 87
Query: 84 AWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 143
W LYWAAQG + ++VL H+CGH +FSD L+ VG ILHSF++VPY W+ SHR H
Sbjct: 88 LWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTH 147
Query: 144 HSNTGSLERDEVFVPKQKSALSWFSKYLNNPPG-RVLTLAVTL-TLGWPLYLAFNISGRP 203
H N G +E DE +VP + Y P R+L V L L +PLYL + G+
Sbjct: 148 HQNHGHVENDESWVPLPERV------YKKLPHSTRMLRYTVPLPMLAYPLYLCYRSPGKE 207
Query: 204 YNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCVYGVPLLIV 263
HF+PY +++ ER I S + L L+ G V+ VYGVP +I
Sbjct: 208 ----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVPYIIF 267
Query: 264 NGFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNITDTHVAHHL 323
+L +T+L H LP Y EW ++RG L T+DRDYGI N + H+I THV HHL
Sbjct: 268 VMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDI-GTHVIHHL 327
Query: 324 VSTMPHYHAMEATNAIKPILGQYY 344
+PHYH ++AT A K +LG+YY
Sbjct: 328 FPQIPHYHLVDATKAAKHVLGRYY 333
BLAST of Sed0013473 vs. TAIR 10
Match:
AT3G11170.1 (fatty acid desaturase 7 )
HSP 1 Score: 212.6 bits (540), Expect = 5.5e-55
Identity = 126/356 (35.39%), Postives = 185/356 (51.97%), Query Frame = 0
Query: 24 PSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQNLPSLLFYP 83
P PPF L +++ AIP HC+ ++ +S SYVV D +A +F A + N +
Sbjct: 90 PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRD--VAIVFALAAGAAYLN-----NWI 149
Query: 84 AWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRH 143
W LYW AQG + ++VL H+CGH +FS+ L+ VG +LHS ++VPY W+ SHR H
Sbjct: 150 VWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTH 209
Query: 144 HSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVLTLAVTLT-LGWPLYLAFNISGRPY 203
H N G +E DE + P + + K P R + L L +P YL G+
Sbjct: 210 HQNHGHVENDESWHPMSEKIYNTLDK-----PTRFFRFTLPLVMLAYPFYLWARSPGKK- 269
Query: 204 NRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCVYGVPLLIVN 263
H+ P ++ +ER + S + +L L G ++ +YG+P I
Sbjct: 270 ---GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINV 329
Query: 264 GFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNITDTHVAHHLV 323
+L +T+L H LP Y EW ++RG L T+DRDYG++N + H+I THV HHL
Sbjct: 330 MWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHHLF 389
Query: 324 STMPHYHAMEATNAIKPILGQYYHFDGTP------FVKAMWREAKECIYVAPDEGE 371
+PHYH +EAT A KP+LG+YY ++ + + KE YV+ DEGE
Sbjct: 390 PQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS-DEGE 427
BLAST of Sed0013473 vs. TAIR 10
Match:
AT5G05580.1 (fatty acid desaturase 8 )
HSP 1 Score: 204.1 bits (518), Expect = 1.9e-52
Identity = 118/332 (35.54%), Postives = 172/332 (51.81%), Query Frame = 0
Query: 15 SDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLILASIFYYIATTYFQ 74
S+ D + P PPF L +++ AIP HC+ ++ S SYVV D+ + +A YF
Sbjct: 74 SEEDTERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVA-AYFN 133
Query: 75 NLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDDTVGLILHSFLMVPYF 134
N + W LYW AQG + ++VL H+CGH +FS+ L+ G +LHS ++VPY
Sbjct: 134 N------WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYH 193
Query: 135 SWKYSHRRHHSNTGSLERDEVFVPKQKSALSWFSKYLNNPPGRVLTLAVTLTLGWP-LYL 194
W+ SHR HH N G +E DE + P +S K T TL +P L
Sbjct: 194 GWRISHRTHHQNHGHVENDESWHPLPESIYKNLEK---------TTQMFRFTLPFPMLAY 253
Query: 195 AFNISGRPYNRFACHFDPYGPIYSDRERLQIFISDVGLLTVTYVLYRLALAKGIAWVVCV 254
F + R + H+ P ++ +E+ + S + +L L G ++ +
Sbjct: 254 PFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKL 313
Query: 255 YGVPLLIVNGFLVLITFLQH--THPALPHYDSSEWDWMRGALATVDRDYGILNKVFHNIT 314
YG+P I +L +T+L H LP Y EW ++RG L T+DRDYG +N + H+I
Sbjct: 314 YGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI- 373
Query: 315 DTHVAHHLVSTMPHYHAMEATNAIKPILGQYY 344
THV HHL +PHYH +EAT A KP+LG+YY
Sbjct: 374 GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYY 388
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004136572.1 | 1.6e-210 | 90.84 | delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical ... | [more] |
XP_038903906.1 | 3.6e-210 | 90.58 | delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida] | [more] |
XP_022934859.1 | 4.7e-210 | 91.36 | delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata] | [more] |
XP_008443062.2 | 6.2e-210 | 90.58 | PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 del... | [more] |
KAG6580810.1 | 1.8e-209 | 91.10 | Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbi... | [more] |
Match Name | E-value | Identity | Description | |
Q8GZC3 | 3.7e-189 | 80.37 | Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV... | [more] |
P48631 | 3.1e-188 | 80.68 | Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX... | [more] |
Q84VT2 | 5.0e-186 | 77.32 | Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2 | [more] |
Q39287 | 1.3e-181 | 77.86 | Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 ... | [more] |
P46313 | 1.2e-179 | 75.98 | Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LF25 | 7.9e-211 | 90.84 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1 | [more] |
A0A6J1F3S1 | 2.3e-210 | 91.36 | delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
A0A5A7TLK3 | 3.0e-210 | 90.58 | Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... | [more] |
A0A1S3B762 | 3.0e-210 | 90.58 | delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=... | [more] |
A0A6J1J0W0 | 1.9e-209 | 91.10 | delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1114... | [more] |