Sed0013324 (gene) Chayote v1

BLAST of Sed0013324 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 364.4 bits (934), Expect = 6.3e-97
Identity = 173/198 (87.37%), Postives = 182/198 (91.92%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA+SS AQS  PPVLD EGRPL+  VEYFIKPA+TDVAGNLTLLTR
Sbjct: 3   LCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 62

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL G+DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 63  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 122

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           ET+PTTGRRFVETGDDDTATGYFRID+D FGVYNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 123 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 182

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 183 LRLLALDGSAFPFEFVRA 198

BLAST of Sed0013324 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.4 bits (934), Expect = 6.3e-97
Identity = 173/198 (87.37%), Postives = 182/198 (91.92%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA+SS AQS  PPVLD EGRPL+  VEYFIKPA+TDVAGNLTLLTR
Sbjct: 2   LCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 61

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL G+DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 62  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 121

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           ET+PTTGRRFVETGDDDTATGYFRID+D FGVYNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 122 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 181

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 182 LRLLALDGSAFPFEFVRA 197

BLAST of Sed0013324 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.5 bits (929), Expect = 2.4e-96
Identity = 172/198 (86.87%), Postives = 182/198 (91.92%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA+SS AQS  PPVLD EGRPL+  VEYFIKPAITDVAGNLTLLTR
Sbjct: 3   LCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTR 62

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL G+DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 63  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 122

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           ET+PTTGRRFVETGD+DTATGYFRID+D FG+YNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 123 ETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKG 182

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 183 LRLLALDGSAFPFEFVRA 198

BLAST of Sed0013324 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 355.5 bits (911), Expect = 2.9e-94
Identity = 171/198 (86.36%), Postives = 178/198 (89.90%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA SS AQS  PPVLD EGRPL+  VEYFIKPAITDVAGNLTLLTR
Sbjct: 3   LCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTR 62

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL  +D IREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 63  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVG 122

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           E +PTTGRRFVETGDDDTATGYFRID+D FGVYNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 123 EADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 182

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 183 LRLLALDGSAFPFEFVRA 198

BLAST of Sed0013324 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 354.4 bits (908), Expect = 6.5e-94
Identity = 166/195 (85.13%), Postives = 177/195 (90.77%), Query Frame = 0

Query: 3   KPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKG 62
           K  AIFSSLC FMAISS AQ DGPPVLD EGRPL+R VEYFIKPAITDVAGNLTL+TRKG
Sbjct: 6   KSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKG 65

Query: 63  DQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVGET 122
           DQCPF+VGQVPL SQDIGF VTFTPYL G+DTIREGRDLKVVFQAF+ICITST W+VGE 
Sbjct: 66  DQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQWKVGEA 125

Query: 123 EPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKGLR 182
           +P TGRRFV+ G+DDTATGYFRIDK + GVYNIGWCPSDVPI+GRPRCG AGILIEKGLR
Sbjct: 126 DPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLR 185

Query: 183 LLALDASAFPFEFVR 198
           LLALD SAFPFEFVR
Sbjct: 186 LLALDGSAFPFEFVR 200

BLAST of Sed0013324 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.7e-36
Identity = 86/196 (43.88%), Postives = 119/196 (60.71%), Query Frame = 0

Query: 7   IFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQCP 66
           I +++ L +  +S AQ     V+D  G P++   +YFI+PAIT   G+LTL+TR  + CP
Sbjct: 10  ILANVWLLVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCP 69

Query: 67  FHVGQVPLRSQDIGFPVTFTPYLVGK--DTIREGRDLKVVFQAFSICITSTAWRVGETEP 126
           F+VG  P   Q  GF V  +P++  +  D +R GRDL+V+FQA + C  ST WR+GE + 
Sbjct: 70  FNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDA 129

Query: 127 TTGRRFVETGDDDTATG----YFRI-DKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEK 186
           TTGRRF+ TG DD+  G    +FRI      G++NI WCP++V    +  CG  GI+ E 
Sbjct: 130 TTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVREN 189

Query: 187 GLRLLALDASAFPFEF 196
           G  LLALD SA P  F
Sbjct: 190 GKILLALDGSALPVAF 197

BLAST of Sed0013324 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.3e-32
Identity = 78/196 (39.80%), Postives = 108/196 (55.10%), Query Frame = 0

Query: 7   IFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQCP 66
           I + +CLF+  ++ AQ     VLD  G P++   EYFI+P IT+  G  T+++R  + CP
Sbjct: 12  ILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NESCP 71

Query: 67  FHVGQVPLRSQDIGFPVTFTPYLVGK--DTIREGRDLKVVFQAFSICITSTAWRVGETEP 126
            HVG + L     G  V FTP+      D +R  RDL++ FQA S C+ ST WR+GE + 
Sbjct: 72  LHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKDT 131

Query: 127 TTGRRFVETGDDDTATG----YFRIDKDEF-GVYNIGWCPSDVPIKGRPRCGRAGILIEK 186
            +GRR + TG D    G    +FRI +    G+YNI WCP++V    +  CG   +L E 
Sbjct: 132 KSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNEN 191

Query: 187 GLRLLALDASAFPFEF 196
           G  LLALD    P  F
Sbjct: 192 GKILLALDGGPLPLVF 201

BLAST of Sed0013324 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 8.1e-31
Identity = 79/195 (40.51%), Postives = 107/195 (54.87%), Query Frame = 0

Query: 7   IFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQCP 66
           I + + L MA +S AQ     V+D  G P++   EYFI+PAIT   G  TL+T  G  CP
Sbjct: 10  ILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-PCP 69

Query: 67  FHVGQVPLRSQDIGFPVTFTPYLV--GKDTIREGRDLKVVFQAFSICITSTAWRVGETEP 126
            HVG +      +G  V FTP+      D +R  RDL+V F   + C  ST WR+GE + 
Sbjct: 70  LHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDA 129

Query: 127 TTGRRFVETGDDDTA---TGYFRIDKDEF-GVYNIGWCPSDVPIKGRPRCGRAGILIEKG 186
           T+GRR + TG D+ A     +FRI + +  G YNI WCP++     + +CG  G++ E G
Sbjct: 130 TSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENG 189

Query: 187 LRLLALDASAFPFEF 196
             LLALD  A P  F
Sbjct: 190 KNLLALDGDALPVVF 198

BLAST of Sed0013324 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 95.5 bits (236), Expect = 7.1e-19
Identity = 69/192 (35.94%), Postives = 96/192 (50.00%), Query Frame = 0

Query: 19  STAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTL--LTRKGD-QCPFHVGQVPLR 78
           S A S   PVLD +G  L+ G  Y+I P + D  G LT+   T  G   CP  V Q   +
Sbjct: 27  SAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQT-RK 86

Query: 79  SQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSIC--ITSTAWRVGETEPTTGRRFVET 138
             D   P+ F P    +D +R   DL + F AF  C   +ST WR+ + + +TG+ FV  
Sbjct: 87  EVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTI 146

Query: 139 G------DDDTATGYFRIDKDEF---GVYNIGWCPSDVPIKGRPRCGRAGILI-EKGLRL 196
           G        +T + +F+I  +EF   G Y + +CP+ V    + +CG  GI I +KG R 
Sbjct: 147 GGVKGNPGPETISSWFKI--EEFCGSGFYKLVFCPT-VCGSCKVKCGDVGIYIDQKGRRR 206

BLAST of Sed0013324 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.3e-16
Identity = 57/164 (34.76%), Postives = 80/164 (48.78%), Query Frame = 0

Query: 9   SSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQCPFH 68
           SS  LF     +  +D PPV D +G  L+    Y++  A     G LT+    G  CP  
Sbjct: 9   SSSPLFWPAPPSRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLF 68

Query: 69  VGQVPLRSQDIGFPVTFTPYLVGKD--TIREGRDLKVVFQAFSICITSTAWRVGETEPTT 128
           V Q P   Q  GFPV  TPY V      IR   D+++ F+A++ C+ ST W + ++E   
Sbjct: 69  VSQDP-NGQHDGFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAA 128

Query: 129 GRRFVETG--DDDTATG---YFRIDK------DEFGVYNIG-WC 159
           GRR V TG   D + +G    FRI+K       E+ + + G WC
Sbjct: 129 GRRHVITGPVKDPSPSGRENAFRIEKYSGAEVHEYKLMSCGDWC 170

BLAST of Sed0013324 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.0e-97
Identity = 173/198 (87.37%), Postives = 182/198 (91.92%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA+SS AQS  PPVLD EGRPL+  VEYFIKPA+TDVAGNLTLLTR
Sbjct: 3   LCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 62

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL G+DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 63  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 122

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           ET+PTTGRRFVETGDDDTATGYFRID+D FGVYNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 123 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 182

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 183 LRLLALDGSAFPFEFVRA 198

BLAST of Sed0013324 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.4e-94
Identity = 171/198 (86.36%), Postives = 178/198 (89.90%), Query Frame = 0

Query: 1   MCKPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTR 60
           +CK    FS LCL MA SS AQS  PPVLD EGRPL+  VEYFIKPAITDVAGNLTLLTR
Sbjct: 3   LCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTR 62

Query: 61  KGDQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVG 120
           KGDQCPFHVGQVPLRSQ+IGF VTFTPYL  +D IREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 63  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVG 122

Query: 121 ETEPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKG 180
           E +PTTGRRFVETGDDDTATGYFRID+D FGVYNIGWCPSDVPI+GRPRCGRAGILIEKG
Sbjct: 123 EADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 182

Query: 181 LRLLALDASAFPFEFVRA 199
           LRLLALD SAFPFEFVRA
Sbjct: 183 LRLLALDGSAFPFEFVRA 198

BLAST of Sed0013324 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.8e-90
Identity = 162/196 (82.65%), Postives = 176/196 (89.80%), Query Frame = 0

Query: 3   KPFAIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKG 62
           KP AIFS L LFMAI STAQS  PPVLD +GRPL+RGVEYFIKPA+TDVAGNLTLLTRKG
Sbjct: 7   KPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKG 66

Query: 63  DQCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVGET 122
           D CPFHVGQVPLRS DIG+ +    +L G+DTIREGRDLKVVFQAFSICITSTAWRVGET
Sbjct: 67  DPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWRVGET 126

Query: 123 EPTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKGLR 182
           EPTTGRR V TG+DDTATG+FRIDK + GVYN+GWCPSDVPI+GRPRCGRAGIL+EKG+R
Sbjct: 127 EPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGVR 186

Query: 183 LLALDASAFPFEFVRA 199
           LLALD SAFPFEFVRA
Sbjct: 187 LLALDGSAFPFEFVRA 200

BLAST of Sed0013324 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.7e-87
Identity = 154/192 (80.21%), Postives = 167/192 (86.98%), Query Frame = 0

Query: 6   AIFSSLCLFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQC 65
           A+FS LC FMAI STAQ DGPPVLD EGRPL+R VEYFIKPAITDVAGNLTL+TRKGDQC
Sbjct: 10  ALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQC 69

Query: 66  PFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVGETEPT 125
           PF+VGQVPL SQ+IGF VT TPYL G DTIREGRDLK VFQAF+ICIT T W+VGE +P 
Sbjct: 70  PFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPK 129

Query: 126 TGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKGLRLLA 185
           +GRRFV+ G+D+TATGYFRIDK   GVYNIGWCPSDVP KGRPRCG AGILIEKG+R LA
Sbjct: 130 SGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLA 189

Query: 186 LDASAFPFEFVR 198
           LD  AFPFEFVR
Sbjct: 190 LDGPAFPFEFVR 201

BLAST of Sed0013324 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.7e-84
Identity = 152/194 (78.35%), Postives = 165/194 (85.05%), Query Frame = 0

Query: 5   FAIFSSLCLFMAISSTA-QSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGD 64
           FAIFS LC FMAI STA Q DGPPVLD EGRPL+R  EYFIKPAITDVAGNLTL+TRKGD
Sbjct: 9   FAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGD 68

Query: 65  QCPFHVGQVPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQAFSICITSTAWRVGETE 124
           QCPF+VGQVPL SQ+ GF V+  PY  G+DTIREGRDLK VFQ F+ICIT T W+VGE +
Sbjct: 69  QCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEAD 128

Query: 125 PTTGRRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILIEKGLRL 184
           P TGRRFV+ G D+TATGYFRIDK + GVYNIGWCPSDVPIKGRPRCG AGILIEKG+R 
Sbjct: 129 PKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRF 188

Query: 185 LALDASAFPFEFVR 198
           LALD  AFPFEFVR
Sbjct: 189 LALDGPAFPFEFVR 202

BLAST of Sed0013324 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 85.1 bits (209), Expect = 6.8e-17
Identity = 60/195 (30.77%), Postives = 90/195 (46.15%), Query Frame = 0

Query: 13  LFMAISSTAQSDG-PPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQ 72
           L +A+++   S+    V+D +G  +     Y++ P I    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 73  VPLRSQDIGFPVTFTPYLVGKDTIREGRDLKVVFQ-AFSICITSTAWRVGETEPTTGRRF 132
                 D G PV F+ + +    + E ++L +      +ICI ST WRVGE +    + F
Sbjct: 74  -ESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 133 VETG------DDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILI-EKGLRL 192
           V  G        D+   +F+I+K     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 193 LALDASAFPFEFVRA 199
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

BLAST of Sed0013324 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 51.6 bits (122), Expect = 8.4e-07
Identity = 49/193 (25.39%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 13  LFMAISSTAQSDGPPVLDNEGRPLKRGVEYFIKPAITDVAGNLTLLTRK---GDQCPFHV 72
           +F A S+   +    VLD  G P++  V+Y+I PA     G L    R     D C  ++
Sbjct: 17  IFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLC-LNL 76

Query: 73  GQVPLRSQDI-GFPVTFTPYLVGKDTIREGRDLKVVFQA-FSICITSTAWRVGETEPTTG 132
             V   S  + G PVTF+P       ++    L + F +   +C  S  WR+  +     
Sbjct: 77  DIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQLRK 136

Query: 133 RRFVETGDDDTATGYFRIDKDEFGVYNIGWCPSDVPIKGRPRCGRAGILI-EKGLRLLAL 192
                 G       +F+I +D    Y + +C    PI     C    + I + G+R L L
Sbjct: 137 SFVSIGGQKGKGNSWFQIQEDG-DAYKLMYC----PISSIVACINVSLEIDDHGVRRLVL 196

Query: 193 DA-SAFPFEFVRA 199
               +F  +F +A
Sbjct: 197 STDQSFVVKFQKA 203