Sed0013113 (gene) Chayote v1

Overview
NameSed0013113
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPeptide chain release factor
LocationLG06: 2809161 .. 2816168 (-)
RNA-Seq ExpressionSed0013113
SyntenySed0013113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTACCGTTTAAACGGGGTCTTATCCCCAAATTGTTCAGTCATTTACAGTAAAGAAGTTTTCGTAGGAACGGAAACGAAGAAGAAGAAGAAGCCATGGACGTAGTTCAGAAGCCATTGCAGCTCTGTTGCAGCTTTCCCGTTTTTCCCCTTCCAAGATTTCAATCCACTGCAGCTTCCAGATCCTTACGATTTCCACTTTCAAGCTCTTGCGATTCTTTCTCGGTTCACTCTCGTTTCCCTCTCGGTAGGTTCGATCGATTCCAAGATTTCTTCCTCTTTTTGTCTGTTTGATTGATTTCTCTGCGTTGTTCTGAATCGCATTTGTGTGGTTCTAGGACTCGTTTATTGAGTTTTGGTGTAATGGCGATGGATTTTTTTCAGTTTCTGTAACTTATTTGTTTCAGTAGTTTCATTCGCAAGGCCTGAGAGCCGGGTTGGTGAAGAGGTGGCCTCGGATACGCAAGAATGGGCGATGCAAGGTATGTTCTGTTCTAATGATTGAATTTTAGTAATTACTGTTACAAATTAACGTTTCGATTTGTTGAATATTCTCTGAAGATGAAAGTTTGTTATTCGAATTTTGAACAATGGTTTAGTTTAGAGAATGCTCATTTGTTATCAGGTATCTGGCATTTCTATTTCTTTCTTCTTCTTTTTTTTTTTGTCCTGCTAGGATTATGGAAGATGATAATGTCTTTATTAATAGGATTTAATGGAGTTAAGTAAACATTAAAAGAAATTATATGATTTTTTTTTTAAGTTCAACAAAAGATGAGTATCTTGAATTAATGATAGGTCGCCACCTACCTAAGAATTAATTTCTTACGGATTTTTGGCATTCAAATATGGTAGGATTAGACGGGTTTTCCTGAGAGAATAGTCGAGGTGCGCATAAGGTAGCTCGGATAGTCAAAGATATTTAATAAGAAAAAAGGCAATGTTATAATGTATTTGTTTATAGTTCAGTTCCTAAATAATCAAATAAATATTGCCGTCACATGGTTGAATATTCTCTGAAGATGAAAGTTTGTTATTCGATTTCTGCACAAAGGTTTAGTTTAGAAAATGTTCATTTGTTATCAGGTATCTGGCATTTCCATTTCTTTTTTTGCCCTGCTAGGATTATGGAAGATGATGATGTCTTTGTAAATGTGATATAATGTGTTTTTCTTTCGTTCCATGAATCGAGCTTTCATAAAATGTTATTATATTTTTTAGCAAAACAAATGTTGAGTATCTTGAACCTACAACCTCAAAAGAATTGAAAGAATGCATGTCACAGACGATAAGACGTAATATGGATTTATGTTCGAGAATAATTGAGGTGTGCATATAGGTAACCCGGACAGTCACAGATATTAAAAAAAATACTATAACGTATTTGTTTCGGTTCCTGAATGATAAAATAAATTTTTCGAGTAACTACTGACTACTGACTAGTTGTATCCCATTAATGTGGCTAATGTTATAATATTTCTGTTAGACGATGCTCAATTTCATGGGCTACAGTTCAAAATTCACTTGTGACATTAATTTGTAGATACCTCCCGTGTCTTCGTTCTTTCGATGTCTCCGAGTGTCTGGCTTAGAGACGGGCGTGGTTACCCTGGGTATAATGGAGTGAGCTCCGGTTTCCCAATTTGAAAACAAAAAAAAAAAAATCAAATGGGCTACATGAATCTTCACTTGAATGCTGTTTTTTCTTCTAAATCGATAAGTGTATGGGCTATTTACGCGTAACTCAACTATTCTCACCAGGCATACCTTATGACTCTATCATTTGGATGGGAACAAAACTCGTAGGAAATTATTTTCTAGGGAGGTGGTCACTATATATTGAACTAATAACCTCTTGAATCTCCAATGTCCTCTTTGACCACTAGGCCAACCCATGATGCTTGAATGCCAAATTTCTTAAGCCTTTTTCTTATTTTGTTATGATAAGTCGGAAACTACTAAATAACATCATTAAAATATATATTACTGCTATTTAAAGACACAACAAGCTCTTTATTGTCTTAGATTTTGATTCTACATTTATATTTAGATGCATTTACTTCCCATTTATCCATTTTGGATTCAGTGGTGTTTGCAACTCAATGGAAATGGTATAAGCTGTGCTTGTTGTTGCTGACAGATTTCTACTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCGGGTTTGTCACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCGGCTGACACCTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCATGGCCCTGAATGACGTGAAAGATAAAAGAACGATGCTTACTGACTTCAAAACACAGGTAATTTTTTCAAAATACTGGTAATGTATATGAAGTTTCTAGTATGTTTCATTCATCATCAACTATACAACAGTATAAATACAAATACAATCACTCCAAAAGGGGAAAGAATGTTGTATCCTTTACCATTTCCGCTAACACATCTTAAATAAAATAAATAATTCTAAAAAAAACAAGAATGCCTTAACTTTAGCCTTAATTTATAACAATATATAGCGTGAAATCTATTTGTTAGATTATGCTGTATTTCGTTTGCTGAAGTCAAATATATAGACATACATGGGAGAGTCCTAAACTGAGTGAAATTACAGTGAAGGACAAATGACTAATAAACTCTTGGATCTAACATATCTATATCAATAAACATAATAATATACTTCGATGTCTCCCAGTGTCTGGCCTAGGGCAAGCGTAGTTACTCCGGGCATAGTGGGGTGAAACTTTGATTTGTCAGTTTCCAACAAAAAAAAATAGGCATATTAATATACACATTGTATCACTCCCCTCAAGTTAGTCATGCATAACTTGCTCTAGATGCAATCTCTTCATATCATACCCCATTTAAAGATTTTGTGGAGATATCTTCTATTTGTTCTCCAATCTTGGTTCACCCAAGTATACCATGGCTATGACTGGCTTACTAGCGTATCAGTTGGAATTGTAATATTCGATTTTAAGGCCTAAACTTGCTTCATATGGTGATGTAGAGAATTCATTTTTTCTCTTTTCATTTCTTGTGTTACTATACAATTAAGCCTTAACATTTGGGTGAATAATCTGACCAAAGCTTTTGAAAAAAGGAACGAAATCAACACCTCAGGTGTGGGCTATGTTTTATGATTGATTCAGGTTGAAGAAGCAGAAACGATAGTTAACCTGACTGAGGAGATGGATTCGGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTAAACAAGGCACTGGACCAATTTGAATTGACACAACTTCTTTCCGGTCAGTATGATAAAGAAGGTGCTGTCATCACGATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGTAAATAGTTAAGAGCTTATTTTGAAAACTGTTTCCGAAGTGTTGTTTTTGGGATTTTGTTTTCAAAACTGCTTCTAAGAATTATGGTCGTATATGCACTCGGTGTTTTTAAAAGCACAAGGCCCAATAACGTACTCTAAGGTTTAGGCACAAGGTGCAAAAAAAGCATGGATTTTTCTTAGGTGCACTAGAGAAAAGATAGCATAGTTGGAGTGAGATTATTAAAAAAATATTACTTTAAGGGTTGGTAAATGTTATTTTTTTGTTATTAAAGAACAAAAAACCATAAACTTAAAGTTTTTTGCGTTCAGGATTAGTAAAACTTATTTTCTTATAATTAATTTAGGATAGTGGGCGCCTTTTTACCTACGGTGCTCAAGGAACAAGCAGTGTTTTCAAGGCCACCGCGTTTCCTCGCCTATGGCCATCGCCTTGCACATAGGCAGGTATTGCGCCTCTAACGTAGCTGAGGCGATCTCCTTCAATTAGGCCCGCGCCTTTCCTCGCCATTTTGCAGCCTGCATCGCCTTCGCCTCAGGCCAAGGCGTGCCCCTTGTTAAAGTTTTATTTATTTATCAATATCCTAACTTAATATCCACAAAAAGAACCTAGAATTAGTTTATGAAGAAGTCCAACAACTATGCAAAGTCTTTATCAAGGATACTGAATTTTTTTAATTGCTATTTTAAATATATGTGTGTCTGTTTATTCAAGTTATTTGATATAGAATCTTTGTTTTTTTATACATTTAGTCAACTTCAATTTTTTTATACAAATAATAATTTAATATTATGGTATATCTTACAATAACATAATTTTTGTGATTTATTATTCTTTTATTTTTTACTTTTTAGTATTTTTCCATATGAATTTGAAATTTTATGGGAATGCACCTATGTTTTATTATCAATTTACAAATGCGCATCCCTTCACTCGAGCCTCGCCTTTTTAATGCCTCTTGCTTTGAGGCGATCAAGAAGAGTATCGCCTTGCGTTGCACCTTGCGCTTTGAAAACATTGGGCACAAGGCAAGACGTTAGACCTACACTTTGCGCCTAGGCTGGAGTTTGACCGATGCCGGGAATTAGTTGATTTTAAACTTCAGTGTTTTCGCTAAACTTATTGCTTCTCCGGATTGAATTTTTGATAATCTTCAGTGCAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGGTACAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACACACAGGATTGTGCGACAGTCACCTTTTAACTCTAAAGGACTTCGACAGGTAATGACTAATTGTAACACTTTCCCGAAGATCACTGGTGCTTGGGATATTAAGAAAATGGGAAAAGTATTTATTTAGTCCCTAAGTTTGGGATGTAGTTTTTATTTTGTATCTATGTTTCAAAATTACACTTTTACCCCTCACATTTCAATAATGTTCAATTTAGTCTTTATATGTTTTTGAGTTTACTTTCATATTTATTTTCTTCTAAAAATTAAATTCAGTTCAAGACAAAAATAATCACTTCAACCTTCATTAAAATTTAAAATTAGTCAAGTCACTATATTAGTCAACATTTCGTTTTATAACTTACGTTTTTGTTATCTTAAGCTCTAAATTTGAGCATTAAAATGTGAGGGGTAAAAATGTTTGATTTTGAAACTAGGAATCAAACATAAACTATACTCAAAACCTACCTAGGGACTAAAAATGTTATTTTTCCTCAGAAAAAATAGACTATGAAGGGGCCTAACGCCATTATGTCTTAAAATTTTCATGTACTGAAAATTAGAATCCAGAATCATATATCATTGACTTTTAATGATTTTATGTGAAACTAAGTGACCATTTAGTCACGTTTCCTCAAGATAGATTGTTTGATGCGTGAGCCTATCTGTTTTAATACAAACTCTATGCAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGACTCCACGAATGTCGAACTGCCAGAAGAGGACTTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGGATTACTCATATCCCGACAGGCGTCACACTTCGCTGCACAGGTCTTCTATCCTACTTTAACTACCCTTATTTATGCTACCATATAAACATGATGTCTGTTCTGTTCGTAAGAGATAATATTAGGACAATGTCCTTGACTTTTATCAAAATTGTTCAAAATGATTTATCACTTATAGCAGAATCATTTCACTAGGAAGCTGTGATAAAATCAATTGATTGAAATGTGATGTTATCATGCTAAGTATTAATTTTGTTTGAAATAAATTTAATTTAAATAGTAGTAGGTTTTGTTTGATAATCATTAGTTTTCAAAAATTTGGTTACCATCATAGTGTGACCTAGTGGTTAAGAGAGTCATGGAAACTAAGAGGTGATGGGTTCCATCTATGGTGGCCACACCTACCTAGGAATTAATTTTCTTCAGGTTTTCTTGACATTCTAATGTTGTAAGGTCAAACAGTAGGTGCATGCAATCTGGCCTGGATAGTCACGAATATAAAAAAAAGGCTTGTTTTCTTTCATTTTCTATACAATAATTTTCATCCTCCTTGAGGAAACATTTGAATTCTTAACCTAATTTTAAAAAACAAAATTTCCCAAAACTACCTTTTCAAGTTTACAAATCTTGGCTAAATTATTGAAAACGTTAGCATAAAGTAGATCAAAGAAACTCAAAATTTTTGCTTATTTATGAGTTTAATTTTTAAACATAGAAAAAAAAACCAAATAGACCAGATATCTTTTCATTTGACTAACAAACCTTTCAGACTTCTCCATGTTGCATGGTTTTAATAATTCAACCTGGCTGGTGCAAGTTTTGATTGAATCAAATATTTCTTCACAAATTCCATTGTATGATCTCTGAACTAATGGCATGGCATTTCATCTTCTTTTTTTTCTCAACGATAATGCAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGGTTGAAAGCCAAGCTGTTAGTGATAGCCGAGGAGCAACGCGCATCTGAAATCAAGCAAATACGAGGAGACACCGTAAAGGCGGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGAACAGGATATGAAACATCAGACATTGTGTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAACACAAATACAATATGGGAGTATCTACTGGTGAGGTTAACTAAAACTGCCATAAACTAATAATAGTTAGCTTTGATTGATTTGGTGTATTGTGTATTATGTATTTCTCTTTTGTAATATGGTGAAAGGGGCAAAGGGCATTAGCAAAGTTCTTGAACCTTGCAAGCTCTGTGAATTTTGTTTTGTTATGAATATAAATGCTTTCCATTATTTACATATATCTCCATTCTTTTGTTAGTTTCATCTGTAGTGAGCCGA

mRNA sequence

GTTTACCGTTTAAACGGGGTCTTATCCCCAAATTGTTCAGTCATTTACAGTAAAGAAGTTTTCGTAGGAACGGAAACGAAGAAGAAGAAGAAGCCATGGACGTAGTTCAGAAGCCATTGCAGCTCTGTTGCAGCTTTCCCGTTTTTCCCCTTCCAAGATTTCAATCCACTGCAGCTTCCAGATCCTTACGATTTCCACTTTCAAGCTCTTGCGATTCTTTCTCGGTTCACTCTCGTTTCCCTCTCGGTAGTAGTTTCATTCGCAAGGCCTGAGAGCCGGGTTGGTGAAGAGGTGGCCTCGGATACGCAAGAATGGGCGATGCAAGATTTCTACTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCGGGTTTGTCACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCGGCTGACACCTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCATGGCCCTGAATGACGTGAAAGATAAAAGAACGATGCTTACTGACTTCAAAACACAGGTTGAAGAAGCAGAAACGATAGTTAACCTGACTGAGGAGATGGATTCGGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTAAACAAGGCACTGGACCAATTTGAATTGACACAACTTCTTTCCGGTCAGTATGATAAAGAAGGTGCTGTCATCACGATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGGTACAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACACACAGGATTGTGCGACAGTCACCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGACTCCACGAATGTCGAACTGCCAGAAGAGGACTTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGGATTACTCATATCCCGACAGGCGTCACACTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGGTTGAAAGCCAAGCTGTTAGTGATAGCCGAGGAGCAACGCGCATCTGAAATCAAGCAAATACGAGGAGACACCGTAAAGGCGGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGAACAGGATATGAAACATCAGACATTGTGTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAACACAAATACAATATGGGAGTATCTACTGGTGAGGTTAACTAAAACTGCCATAAACTAATAATAGTTAGCTTTGATTGATTTGGTGTATTGTGTATTATGTATTTCTCTTTTGTAATATGGTGAAAGGGGCAAAGGGCATTAGCAAAGTTCTTGAACCTTGCAAGCTCTGTGAATTTTGTTTTGTTATGAATATAAATGCTTTCCATTATTTACATATATCTCCATTCTTTTGTTAGTTTCATCTGTAGTGAGCCGA

Coding sequence (CDS)

ATGCAAGATTTCTACTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCGGGTTTGTCACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCGGCTGACACCTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCATGGCCCTGAATGACGTGAAAGATAAAAGAACGATGCTTACTGACTTCAAAACACAGGTTGAAGAAGCAGAAACGATAGTTAACCTGACTGAGGAGATGGATTCGGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTAAACAAGGCACTGGACCAATTTGAATTGACACAACTTCTTTCCGGTCAGTATGATAAAGAAGGTGCTGTCATCACGATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGGTACAAAACAAGAGTAGTTGAGAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTATGGATATATATCTGGGGAGAAAGGAACACACAGGATTGTGCGACAGTCACCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTCATGCCTCTCCTTCCCGACGACTCCACGAATGTCGAACTGCCAGAAGAGGACTTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGGATTACTCATATCCCGACAGGCGTCACACTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGGTTGAAAGCCAAGCTGTTAGTGATAGCCGAGGAGCAACGCGCATCTGAAATCAAGCAAATACGAGGAGACACCGTAAAGGCGGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGAACAGGATATGAAACATCAGACATTGTGTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAACACAAATACAATATGGGAGTATCTACTGGTGAGGTTAACTAA

Protein sequence

MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKHKYNMGVSTGEVN
Homology
BLAST of Sed0013113 vs. NCBI nr
Match: KAG7036800.1 (Peptide chain release factor PrfB1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 690.3 bits (1780), Expect = 9.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 73  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 132

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 133 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 192

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 193 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 252

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 253 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 312

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 313 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 372

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 373 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 432

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 433 IKAYLKYKYSMALATGEVN 451

BLAST of Sed0013113 vs. NCBI nr
Match: XP_022998733.1 (peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 690.3 bits (1780), Expect = 9.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 73  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 132

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 133 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 192

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 193 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 252

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 253 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 312

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 313 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 372

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 373 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 432

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 433 IKAYLKYKYSMALATGEVN 451

BLAST of Sed0013113 vs. NCBI nr
Match: XP_023525877.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.3 bits (1780), Expect = 9.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 72  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 131

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 132 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 191

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 192 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 251

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 252 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 311

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 312 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 371

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 372 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 431

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 432 IKAYLKYKYSMALATGEVN 450

BLAST of Sed0013113 vs. NCBI nr
Match: XP_023525876.1 (peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.3 bits (1780), Expect = 9.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 73  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 132

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 133 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 192

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 193 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 252

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 253 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 312

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 313 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 372

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 373 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 432

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 433 IKAYLKYKYSMALATGEVN 451

BLAST of Sed0013113 vs. NCBI nr
Match: XP_022949524.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 690.3 bits (1780), Expect = 9.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 72  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 131

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 132 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 191

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 192 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 251

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 252 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 311

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 312 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 371

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 372 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 431

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 432 IKAYLKYKYSMALATGEVN 450

BLAST of Sed0013113 vs. ExPASy Swiss-Prot
Match: Q9LVY0 (Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB1 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.2e-173
Identity = 303/379 (79.95%), Postives = 344/379 (90.77%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA DTSFWDDR+KAQ+ L +L
Sbjct: 78  MQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSL 137

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
           ND+KD+  +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQL
Sbjct: 138 NDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQL 197

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKS
Sbjct: 198 LSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKS 257

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           AT+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+++  +E+PEE
Sbjct: 258 ATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEE 317

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DL+ISF+RAGGKGGQNVNKVETAVRITHIPTGV +RCTEERSQLANK +AL RLKAKL+V
Sbjct: 318 DLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMV 377

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRA+EIK+IRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PF
Sbjct: 378 IAEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPF 437

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLKHKY + +++   N
Sbjct: 438 IKAYLKHKYTLAMASAVTN 456

BLAST of Sed0013113 vs. ExPASy Swiss-Prot
Match: P74476 (Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prfB PE=3 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 1.9e-116
Identity = 213/361 (59.00%), Postives = 274/361 (75.90%), Query Frame = 0

Query: 7   LRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVKDK 66
           L++ +E  S R+ + ++   L  L  ++ DLE  AA   FWDD  +AQQ+L  LN+ K +
Sbjct: 8   LKRNLELISSRLGQTQDYLDLPGLKAKVQDLEQCAAQPDFWDDTDQAQQILQTLNETKSQ 67

Query: 67  RTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQYD 126
                 ++ Q ++++ IV L E  D  D  LL EA + ++ L K LD++EL QLLSG YD
Sbjct: 68  LEQWGIWQQQWQDSQAIVELLELED--DQALLTEAETTLEQLQKELDRWELQQLLSGPYD 127

Query: 127 KEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 186
            +GA +TI+AGAGGTDAQDWA+MLLRMY RW EKQ YK  + E S G+EAG+KS T+EIE
Sbjct: 128 AKGATLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVHLAEISEGDEAGLKSVTLEIE 187

Query: 187 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDLEISF 246
           GRYAYGY+  EKGTHR+VR SPFN+ G RQTSF+GVEVMPLL +++ ++++P++DL+IS 
Sbjct: 188 GRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAISLDIPDKDLDIST 247

Query: 247 SRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQR 306
           SRAGGKGGQNVNKVETAVRI H+PTG+ +RCT+ERSQL NK KAL+ LKAKLL++ EEQR
Sbjct: 248 SRAGGKGGQNVNKVETAVRIVHLPTGLAVRCTQERSQLQNKEKALAILKAKLLIVLEEQR 307

Query: 307 ASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 366
           A  I +IRGD V+A WG QIRNYVFHPY+LVKD+RT  ET+D+  VMDGEL  FI+AYL+
Sbjct: 308 AQAIAEIRGDMVEAAWGTQIRNYVFHPYQLVKDLRTNVETTDVGGVMDGELSDFIEAYLR 366

Query: 367 H 368
           H
Sbjct: 368 H 366

BLAST of Sed0013113 vs. ExPASy Swiss-Prot
Match: B7K0A6 (Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=prfB PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.0e-114
Identity = 209/360 (58.06%), Postives = 273/360 (75.83%), Query Frame = 0

Query: 7   LRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVKDK 66
           L++E++  + R+ + ++   L  L+  + DLE  AA   FWD++  AQ+ L  LND+K  
Sbjct: 6   LKREIQQIAARLGKTQDYLDLPALNANIQDLEQIAAQPDFWDNQETAQKTLQQLNDLKSS 65

Query: 67  RTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQYD 126
                 +  Q+E+ + I  L E  +  D  L EEA + +  LN  LD++EL +LLSG YD
Sbjct: 66  VEEYHQWMGQLEDLKAIAELLELEE--DATLNEEAEANLTQLNHELDRWELQRLLSGIYD 125

Query: 127 KEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 186
            +GAV+TI+AGAGGTDAQDWA+MLLRMY RWGE+Q YK  + E S G+EAGIKSAT+EIE
Sbjct: 126 SKGAVLTINAGAGGTDAQDWAEMLLRMYTRWGEQQGYKVHLTEISEGDEAGIKSATLEIE 185

Query: 187 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDLEISF 246
           GRYAYGY+ GEKGTHR+VR SPFN+ G RQTSF+G+EVMP L ++   VE+PE+DLEI+ 
Sbjct: 186 GRYAYGYLKGEKGTHRLVRISPFNANGKRQTSFAGIEVMPALEEEDLKVEIPEKDLEITT 245

Query: 247 SRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQR 306
           +R+GGKGGQNVNKVETAVR+ H+PTG+ +RCT+ERSQL NK KAL+ LKAKLL+IA+EQR
Sbjct: 246 TRSGGKGGQNVNKVETAVRVVHLPTGIAVRCTQERSQLQNKEKALALLKAKLLIIAQEQR 305

Query: 307 ASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 366
           A  I +IRGD V+A WG QIRNYVFHPY++VKDVRT  ET+D+  VMDG+L+ FI++YL+
Sbjct: 306 AQAIAEIRGDMVEAAWGNQIRNYVFHPYQMVKDVRTNTETTDVNGVMDGKLDLFIESYLR 363

BLAST of Sed0013113 vs. ExPASy Swiss-Prot
Match: Q7U3W6 (Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=prfB PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 7.1e-108
Identity = 203/364 (55.77%), Postives = 264/364 (72.53%), Query Frame = 0

Query: 3   DFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALND 62
           D    ++++   ++R+   ++   +  L     DLE  AA   FWDD+  AQ+ +  L++
Sbjct: 2   DLTDFKRDLSELTDRLGHAQDCLDVPALKARQQDLEQLAAQPDFWDDQQAAQKQMRRLDE 61

Query: 63  VKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLS 122
           VK +   L D+   V++A+  + L E     D  +L EA   +  L + LD++EL +LLS
Sbjct: 62  VKAQLQQLADWGGAVDDAKATLELYEL--EPDEEMLTEAQEGLNQLRQGLDRWELERLLS 121

Query: 123 GQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSAT 182
           G YDKEGAV+TI+AGAGGTDAQDWA MLLRMY RW E    K  V E S GEEAGIKS T
Sbjct: 122 GDYDKEGAVLTINAGAGGTDAQDWAQMLLRMYTRWAEDHGMKVTVDELSEGEEAGIKSCT 181

Query: 183 IEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDL 242
           IE+EGRYAYGY+  EKGTHR+VR SPFN+   RQTSF+G+EVMP + D+  ++++PE+DL
Sbjct: 182 IEVEGRYAYGYLRNEKGTHRLVRISPFNANDKRQTSFAGIEVMPKI-DEEVDIDIPEKDL 241

Query: 243 EISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIA 302
           E++ SR+GG GGQNVNKVETAVRI HIPTG+ +RCT+ERSQL NK KA++ LKAKLLVIA
Sbjct: 242 EVTTSRSGGAGGQNVNKVETAVRILHIPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIA 301

Query: 303 EEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIK 362
           +EQRA+EI  IRGD V+A WG QIRNYVFHPY++VKD+RT  ET+D+ +VMDG L+PFI 
Sbjct: 302 QEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTSEETNDVQAVMDGALDPFID 361

Query: 363 AYLK 367
           A L+
Sbjct: 362 ASLR 362

BLAST of Sed0013113 vs. ExPASy Swiss-Prot
Match: O67695 (Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prfB PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 9.6e-89
Identity = 166/362 (45.86%), Postives = 244/362 (67.40%), Query Frame = 0

Query: 7   LRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVKDK 66
           L+ +VE   +R+E+++      +L+ EL +L+ K ++ +FW+D+ KA+QV+     V++ 
Sbjct: 6   LKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEET 65

Query: 67  RTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQYD 126
              L + +  V++ E +V +T E D+    +++E    IK++ + L + EL   LSG+ D
Sbjct: 66  LNKLKNLEKSVKDLEELVEITSEEDTETWAMMDEE---IKEVERTLRELELKTYLSGEMD 125

Query: 127 KEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 186
            + A +TI AGAGGT+A DWADML RMY RW EK+ Y+  +++ +  + AGIKS T+ ++
Sbjct: 126 AKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVLVK 185

Query: 187 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDLEISF 246
           G YAYGY+ GE+G HR+VR SPF++   R TSF+ V VMP + D+   +E+  EDL+I  
Sbjct: 186 GPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQI-DEDIKIEIKPEDLKIET 245

Query: 247 SRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQR 306
            RA G GGQ VNK +TAVRITHIPTG+T+ C +ERSQ  NK KAL  LKAKL  +  ++ 
Sbjct: 246 FRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKL 305

Query: 307 ASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 366
             + KQ  G+     WG QIR+YVFHPYKL+KD+RTGYET ++ +VMDGE++ FI++YLK
Sbjct: 306 EEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLK 363

Query: 367 HK 369
            K
Sbjct: 366 WK 363

BLAST of Sed0013113 vs. ExPASy TrEMBL
Match: A0A6J1KF53 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 72  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 131

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 132 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 191

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 192 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 251

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 252 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 311

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 312 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 371

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 372 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 431

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 432 IKAYLKYKYSMALATGEVN 450

BLAST of Sed0013113 vs. ExPASy TrEMBL
Match: A0A6J1KHL0 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 73  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 132

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 133 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 192

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 193 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 252

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 253 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 312

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 313 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 372

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 373 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 432

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 433 IKAYLKYKYSMALATGEVN 451

BLAST of Sed0013113 vs. ExPASy TrEMBL
Match: A0A6J1GD28 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 72  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 131

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 132 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 191

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 192 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 251

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 252 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 311

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 312 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 371

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 372 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 431

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 432 IKAYLKYKYSMALATGEVN 450

BLAST of Sed0013113 vs. ExPASy TrEMBL
Match: A0A6J1GCA2 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.6e-195
Identity = 357/379 (94.20%), Postives = 368/379 (97.10%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+ SFWDDRSKAQQVLMAL
Sbjct: 73  MQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMAL 132

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 133 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 192

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 193 LSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 252

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VE+PEE
Sbjct: 253 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEE 312

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 313 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 372

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDIVSVMDGELEPF
Sbjct: 373 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPF 432

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M ++TGEVN
Sbjct: 433 IKAYLKYKYSMALATGEVN 451

BLAST of Sed0013113 vs. ExPASy TrEMBL
Match: A0A6J1D3Q2 (peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016981 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.9e-195
Identity = 358/379 (94.46%), Postives = 367/379 (96.83%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFYSLRKEVETTSERVEEIRNSAGL QLDQELADLESKAAD + WDDRSKAQQVL+AL
Sbjct: 80  MQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVAL 139

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
            DVKDK  MLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL
Sbjct: 140 TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 199

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS
Sbjct: 200 LSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 259

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPD+S +VELPEE
Sbjct: 260 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEE 319

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLV
Sbjct: 320 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLV 379

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF
Sbjct: 380 IAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 439

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLK+KY+M +STG VN
Sbjct: 440 IKAYLKYKYSMALSTGGVN 458

BLAST of Sed0013113 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-174
Identity = 303/379 (79.95%), Postives = 344/379 (90.77%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA DTSFWDDR+KAQ+ L +L
Sbjct: 78  MQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSL 137

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
           ND+KD+  +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQL
Sbjct: 138 NDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQL 197

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKS
Sbjct: 198 LSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKS 257

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           AT+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+++  +E+PEE
Sbjct: 258 ATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEE 317

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DL+ISF+RAGGKGGQNVNKVETAVRITHIPTGV +RCTEERSQLANK +AL RLKAKL+V
Sbjct: 318 DLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMV 377

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRA+EIK+IRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PF
Sbjct: 378 IAEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPF 437

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLKHKY + +++   N
Sbjct: 438 IKAYLKHKYTLAMASAVTN 456

BLAST of Sed0013113 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-174
Identity = 303/379 (79.95%), Postives = 344/379 (90.77%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA DTSFWDDR+KAQ+ L +L
Sbjct: 77  MQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSL 136

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
           ND+KD+  +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQL
Sbjct: 137 NDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQL 196

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKS
Sbjct: 197 LSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKS 256

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           AT+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+++  +E+PEE
Sbjct: 257 ATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEE 316

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLV 300
           DL+ISF+RAGGKGGQNVNKVETAVRITHIPTGV +RCTEERSQLANK +AL RLKAKL+V
Sbjct: 317 DLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMV 376

Query: 301 IAEEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPF 360
           IAEEQRA+EIK+IRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PF
Sbjct: 377 IAEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPF 436

Query: 361 IKAYLKHKYNMGVSTGEVN 380
           IKAYLKHKY + +++   N
Sbjct: 437 IKAYLKHKYTLAMASAVTN 455

BLAST of Sed0013113 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 446.0 bits (1146), Expect = 2.9e-125
Identity = 222/279 (79.57%), Postives = 253/279 (90.68%), Query Frame = 0

Query: 1   MQDFYSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMAL 60
           MQDFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA DTSFWDDR+KAQ+ L +L
Sbjct: 78  MQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSL 137

Query: 61  NDVKDKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQL 120
           ND+KD+  +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQL
Sbjct: 138 NDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQL 197

Query: 121 LSGQYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKS 180
           LSG YDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKS
Sbjct: 198 LSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKS 257

Query: 181 ATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEE 240
           AT+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+++  +E+PEE
Sbjct: 258 ATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEE 317

Query: 241 DLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTE 280
           DL+ISF+RAGGKGGQNVNKVETAVRITHIPTGV +RCT+
Sbjct: 318 DLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTD 356

BLAST of Sed0013113 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 246.1 bits (627), Expect = 4.4e-65
Identity = 134/351 (38.18%), Postives = 207/351 (58.97%), Query Frame = 0

Query: 15  SERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVKDKRTMLTDFK 74
           ++ ++ I+      +L   L  L ++   +  WDD + A ++      +  K   +  F+
Sbjct: 123 AQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFE 182

Query: 75  TQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQYDKEGAVITI 134
            ++ E   ++ L +E +  D+ L  E    + D+ +   + EL  LLS   D     I +
Sbjct: 183 RELLEHIDMLKLAKEEN--DSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEV 242

Query: 135 SAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYI 194
            AGAGGT++ DWA M++ MY  W +++++   VV+++ GE AGIK ATI++ G YAYGY 
Sbjct: 243 QAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYA 302

Query: 195 SGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDLEISFSRAGGKGG 254
             E G HR+VR SPF+S   R TSF+ V V+P+L D ST VE+ + DL I   R+GG GG
Sbjct: 303 KAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGG 362

Query: 255 QNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIR 314
           Q+ N  ++AVRI HIPTG+T  C  ERSQ +NK  A++ L+++L  +   ++ +   Q  
Sbjct: 363 QHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHT 422

Query: 315 GDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYL 366
               +  WG QIR YV HPY++VKD+RT YE SD  SV++G+L+ FI ++L
Sbjct: 423 QSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFL 471

BLAST of Sed0013113 vs. TAIR 10
Match: AT3G57190.1 (peptide chain release factor, putative )

HSP 1 Score: 211.8 bits (538), Expect = 9.3e-55
Identity = 129/363 (35.54%), Postives = 212/363 (58.40%), Query Frame = 0

Query: 5   YSLRKEVETTSERVEEIRNSAGLSQLDQELADLESKAADTSFWDDRSKAQQVLMALNDVK 64
           +SL+K+++    + E     A L   +++    E        WDD +K+ ++L+ L D  
Sbjct: 48  FSLKKKIKDVVLKAEMFAPDA-LELEEEQWIKQEETMRYFDLWDDPAKSDEILLKLADRA 107

Query: 65  DKRTMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGQ 124
                L D K + EEA+ I+ L  EMD++D  L E+A     D++++L  +E+++LL  Q
Sbjct: 108 KAVDSLKDLKYKAEEAKLIIQL-GEMDAIDYSLFEQAYDSSLDVSRSLHHYEMSKLLRDQ 167

Query: 125 YDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSA--GEEAGIKSAT 184
           YD EGA + I +G+ G  +Q W + ++ MY++W E+     RV EK +    ++G+ SAT
Sbjct: 168 YDAEGACMIIKSGSPGAKSQIWTEQVVSMYIKWAERLGQNARVAEKCSLLSNKSGVSSAT 227

Query: 185 IEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDDSTNVELPEEDL 244
           IE E  +AYGY+ GE+G HR++  S  N     +   + V+++PL    S + E+ E DL
Sbjct: 228 IEFEFEFAYGYLLGERGVHRLIISSTSN-----EECSATVDIIPLFLRASPDFEVKEGDL 287

Query: 245 EISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIA 304
            +S+        ++    E  V I HIP+GVTL+ + ER++ AN+IKAL+RLKAKLLVIA
Sbjct: 288 IVSY-----PAKEDHKIAENMVCIHHIPSGVTLQSSGERNRFANRIKALNRLKAKLLVIA 347

Query: 305 EEQRASEIKQIRGDTVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIK 364
           +EQ+ S++ +I    +  E  ++ R+YV   +K+V D +TG E  D+ SV+DG + P + 
Sbjct: 348 KEQKVSDVNKIDSKNI-LEPREETRSYVSKGHKMVVDRKTGLEILDLKSVLDGNIGPLLG 397

Query: 365 AYL 366
           A++
Sbjct: 408 AHI 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036800.19.6e-19594.20Peptide chain release factor PrfB1, chloroplastic [Cucurbita argyrosperma subsp.... [more]
XP_022998733.19.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita maxima][more]
XP_023525877.19.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo sub... [more]
XP_023525876.19.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo sub... [more]
XP_022949524.19.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
Q9LVY02.2e-17379.95Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
P744761.9e-11659.00Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O... [more]
B7K0A63.0e-11458.06Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431... [more]
Q7U3W67.1e-10855.77Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=... [more]
O676959.6e-8945.86Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prf... [more]
Match NameE-valueIdentityDescription
A0A6J1KF534.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima... [more]
A0A6J1KHL04.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima... [more]
A0A6J1GD284.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moscha... [more]
A0A6J1GCA24.6e-19594.20peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moscha... [more]
A0A6J1D3Q27.9e-19594.46peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT5G36170.11.5e-17479.95high chlorophyll fluorescent 109 [more]
AT5G36170.21.5e-17479.95high chlorophyll fluorescent 109 [more]
AT5G36170.32.9e-12579.57high chlorophyll fluorescent 109 [more]
AT1G56350.14.4e-6538.18Peptide chain release factor 2 [more]
AT3G57190.19.3e-5535.54peptide chain release factor, putative [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 98..118
NoneNo IPR availableGENE3D3.30.160.20coord: 228..324
e-value: 2.3E-87
score: 293.7
NoneNo IPR availableGENE3D3.30.70.1660coord: 132..362
e-value: 2.3E-87
score: 293.7
NoneNo IPR availableGENE3D1.20.58.410Release factorcoord: 9..125
e-value: 1.3E-11
score: 46.5
NoneNo IPR availablePANTHERPTHR43116:SF3PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATEDcoord: 1..372
NoneNo IPR availablePANTHERPTHR43116PEPTIDE CHAIN RELEASE FACTOR 2coord: 1..372
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 30..369
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 85..197
e-value: 4.6E-41
score: 152.4
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 31..222
e-value: 2.4E-51
score: 174.3
IPR004374Peptide chain release factor 2TIGRFAMTIGR00020TIGR00020coord: 5..368
e-value: 4.5E-124
score: 412.0
IPR004374Peptide chain release factor 2HAMAPMF_00094Rel_fac_2coord: 3..369
score: 36.456596
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 233..341
e-value: 2.0E-34
score: 118.0
IPR000352Peptide chain release factor class IPROSITEPS00745RF_PROK_Icoord: 248..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0013113.1Sed0013113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006415 translational termination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity