Sed0013090 (gene) Chayote v1

Overview
NameSed0013090
Typegene
OrganismSechium edule (Chayote v1)
Descriptionlow-temperature-induced cysteine proteinase-like
LocationLG01: 21522886 .. 21527626 (+)
RNA-Seq ExpressionSed0013090
SyntenySed0013090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACCTACAAATACAAATCCTCGCCGATTATTGCCTTCCTCCTCTTCCTCACAATAACTACTACATCATCGGCGTTGGAATCTCCGATCATCCCGGCGAGGACGGACGATGAAGTGACGGCGCTGTACGACGACTGGCGCGCCAAGCACGGCAAGTTGCAAAACGGCTTGGATGATCGATTCCAGATATTTAAAGACAATCTCAAATACATCGACGAAATCAACGCCCTAGATTTGCCCTACCGGTTGGGGTTGAATGTTTTCGCAGATCTGACGAACGAGGAGTACCGGTCCAGGAATTTGGGAACCAAGTTCGCGGCCGGTTCGAGGCGGAACAAGACCAGCAGCCGGTATCTGCCTCGGCTTGGGGATGAGCTGCCCGGCTCGGTCGATTGGCGGGAGAAAGGCGCCGTCGCTCCCGTTAAAAACCAGCAGAGTTGTGGTAACTTCCTCTTCTCTCGGCTTCCACTCATTTATTTTCTATTTTGTTTTTTTTCTTTATTCTATTTTCACTATTTTTCTTTTACTTTTTAATCCAATAAAGAAAAAATTGAGATTCGATTCAAGATTGTTGAGGCAAAAAAAGACACCGGAGCAGGGTATGTTAGAATCTCATATTTAAAAATGGAAGAATCCTCCTACAAGATTTTAAAAGAGATATGAGTTGGTTTTGAAGTGGAACCTCCAATTTTCTAATAGATTATTATATATTTGCAATGGATTATAATTGTTATTAGTGATACTATTGTCACTAATTCATTGTTTTTTTTTTTTTTAAGATACTAATCCATTGCTATTATGGTTCTTAAAATGTTCAAAGGTGAATCGGTGATTAAATCTGCCTCCTTTTTCTCCCATTGCTATTTGTTAGATAGCATGAGCATCCATCTAAACTCTCCAAATAGAACAATAATAGAATCTAATAGGGTTTCTCTTAAAAAAGAAAATATGGGTAAAGTAATTTCATGAAATATCATCCAACTTTTGTGAAAATCGAATTTATCGTATAAGATAATAATTATATAATAAATTTGGAGAATTGGGGATTATTTATTTGGTATGAGGTTGTCTCATCTTAGTGGTCCCCATTTCATGTTGGGAACGGTGTTATTGTTAACAATAGTAATCAAAATTGCATCTATATTTTAAAAAAATAATTATATCTCATATTTGTTTTTTTTAAAAAATGATTTACTAGTCGAGTCCTACGTTATGTTGAGTTCTGCTCCTATTTATGTATTTTTTATTAGGTAAAAATTTATGTTGATCATGTAGTTTCAGGCTTTATCTGTTTTAGTTATTTTTTTTTTAGCATTAATAAATGTGATATCTAAAATATATCTTTTGGCTCTTGTGTTTATAGAAATTACTATTTTGTTTCTATTTATAAATTTCTATCAGATGCATGCACAAAAAAAAAAAAAGACTCTAATGTAAAGGTGTGACCATCTTTCAAGAAATTGATGGAGTAAAAGTTGCATTAAATTATGATTGGCAAAATAAACCAGCCAGTTTTTAAAGGATTTTGACAAAAAAGAACTTAAAACTTGGTAGTTTCTTTACTTACCATAATTACGTAATACTTGATCTAAATCATGAAGTCACATATTGAATCATTTCAATATCAGCATCTTTTTTCATCTTTTCTGGTGTTGATTAAACTCATCATCATACTTATTTTTATTCAATTAAAACCATCATATTAAGGGGATGGTTTCAGATCCTCTTATATAGAACTTTATTATAAGTAAGCTGAGAAAATATTAAGTGCTTTAATTTTTTTTAGTATCATAAATCAATTTCGACACTCACCCATGTAAAAGGTATATTTGAAAAGAGTAAACTAAAATATTTATAACTATAGAGCCTAAATTTTGAAATCACTAAACCATCTTGTGTAGTGAAGAACCTATAAAGTTATGAGTTTAATTCAGGCCATCTACTTTAAAATTAACTGATTTTTTTGACATCTAAATATTGTCGGATTCGAAGTGCGTACGTAAGCTAGTCATTGGTCAGTTGAAGTATGCTAATTGTTGAAATTGATATGAAGGGAGTTGCTGGGCGTTCTCAACTATAGCAGCTGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTCATAGCCTTATCAGAACAGGAGCTGGTGGATTGTGACACTACTTATAACGAAGGTTGCAATGGAGGGTTAATGGACTATGCTTTTGAATTCATCATCAACAATGGCGGCATTGACACTGAAGATGATTATCCTTACAAAGGTTTTGATAGCACCTGCATCCAATATAAGGTCTCTTTATTGTCACTTTTCCTTTTGGATATCAGAAGCATATAATTTTGATATCTAATTGTGTTTTTGTTCTGTTTTGTTTCAGAAAAATGCTAGGGTCGTCTCAATTGATGATTATGAAGACCTCCCTGTTAACAATGAGAAAGCACTTCAAAAAGCAACCAGCTAAACAGGTTGTGGCTGTTGCCATTCAAGGAGGTGGTAGAAGTTTCCAGCTATATCATTCGGTATAGTATAACCGCCATTTTTTATGAAATTTTGTTTTTGATTTTAATTTATTTAGAATGTGTTATTTCATCCCAAAATCTATAGTTAGGCTACTTCCTTTTTAATGTGGAATTGTTAACATGTCTGTTCAAGGCAGCTTATGCACATTACGATTATTCTCACGTGATAAACTGTCTGATCCTACAACATTCGATTGTCAAACAAATTCATATAAGAAATTAATTTTTAGATAAATGGTTACAAAGGATTGAACTCCTAACCTTTTAAATTTCCAAAATTCTTTTTAATCACTAGATCAACTTATAATAGTTGATAGTGCATGTTTGGTTTATCAATCTTAGATTGAATCCCAATTTATTTAGGTCATGTTGGATAACCATTTGATTTTTCCTTTTTTGTTTCTAAAATTTAAGTTTGATTTTATTTAAATTCTCTATAAATTGCTTCATATTTTCTTAAGAAAGTAGGTGAATTCTAATCAAATTCCAAAAACCAAAATAGTATTTGAGAACTTTTTTTTTAGTTTTTAAATTTTGGTTTGGTTTTAAAAAACATTGATAAAATATAGATATTAAAGTAAGAAAAATCATATGTTAGAATAGTAGTTTTAGGTTTAAATTTTAAAAATAATAACAAAAAATCAAATGGTTATCAAACAGAGTCTTATTTATTTGTTTTTTTCACCGATTACCCATTTAAACTTGTGAACTCTGGTATCATATTAGACAATATGAGGATTTATCTCAAAATCAATTGATAATGAAAAGACAATCACGTTTGTTGTATGTGCATGTGTTTAGGGTATATTCACAAGAATATGTGGAACAGAATTAGACCATGGTGTTAACGTGGTTGGCTACGGGTCAGAAGGGGGCATTGATTACTGGATTGTAAGAAACTCATGGGGAGCAAGTTGGGGCAAACATGGCTACATCAGGATGCAACGCAACGTGGGTTCATCAAAAGGTCTATGTGGGATTGCAATGCAGCCTTCCTACCCCATTAAAAACGGACCAAACCCTCCAAATCCCGGCCCAACTCCTCCGAGTCCGGTGAAGCCGCCTTCGGTATGCGATGAATATTATACCTGTCCTGCATCTAAAACTTGCTGCTGCATTTTTGAGTTCTCAAAGTTGTGCTTTCAATGGGGATGCTGCCCTCTTGAAAGCGCCACATGTTGTGATGATCATGAGAGCTGCTGCCCCCATGAGTACCCTATCTGTAACGTCTGTGCTGGAACTTGCTTGAAGGTACCTGCCTTCAATTCACATTTTTACATGCATCTAATTGTTTGAACCAGTATCGTAAACAAAAAGAAAAGGGTAAAATAAAATTTTGACTTAACTTCGACATTTGTTAATCAATTTTAATTCTTGTATTTTAAAATTTGTTTCATTTTAGTTCATATACATTCAAAAGTTTAATTTAAGTTCCTAAACTTTTGTTCAGTGTTTGTTCCTAAACTTTTAAGTTTTTTTTAGAGTAAGAGGATTAAAATTGTACTTTTGATAGTTCAAGGACTTAAATTGAACAAACCTCAAAATTCAAGGATTAAGGGTGTATTTGTTTTTGGTAGAAAAGAGTGGAGAGAAAGGGAATGAAGTGGTTAATAGTAGTTTGGAGTGGTCAACTACTCGTTTGGAGATTGAAGTTAATAGTAGTGGAGTAATTAACTACTTGTTTGGGGGGATTAGGGTTAATAGTGGTTGAGTGATAAATTTGATATGAAGTTTAATAAAATTTCATGTGAGATTTGATAGTAGGGTTGGAGAGTGGTTAAAAGTTGTTAAGAGTGGTTATTTACTATAAAAAAACAAAGTGGTTATTAACCACTTTTCATGTCCAAAAAACAAATATACCCTAACATTGGATTTTTTCGAAGTTGAAGGACCAAAATAGAATAAAAGTCAAAGTTGAGTGAACAAAATGTTATTTCATCCTAGAAAATGCATACGTTTTAGGATCTGAACTGTTCGATTCAACTCGAAATGGAGTGCTAACAATTCAAATTGTAAGTTTGAATCGTGAATCACTTGAATAGTACGATTCAAGAGGCCGAATCATTCTATTCTTTCTAAATCGTAACGTTACGACTCTATTATATTTTGTTGTGCAGGGAAAGAACGATGGTTTTGGAGTGAAGGCGATGAGGCGCAGTGTAGCTGCAGCTAAACCATCGTGGGCATTTGGAGATACGACTATCGGAAAGAGTAGTGCTTAA

mRNA sequence

ATGGAAACCTACAAATACAAATCCTCGCCGATTATTGCCTTCCTCCTCTTCCTCACAATAACTACTACATCATCGGCGTTGGAATCTCCGATCATCCCGGCGAGGACGGACGATGAAGTGACGGCGCTGTACGACGACTGGCGCGCCAAGCACGGCAAGTTGCAAAACGGCTTGGATGATCGATTCCAGATATTTAAAGACAATCTCAAATACATCGACGAAATCAACGCCCTAGATTTGCCCTACCGGTTGGGGTTGAATGTTTTCGCAGATCTGACGAACGAGGAGTACCGGTCCAGGAATTTGGGAACCAAGTTCGCGGCCGGTTCGAGGCGGAACAAGACCAGCAGCCGGTATCTGCCTCGGCTTGGGGATGAGCTGCCCGGCTCGGTCGATTGGCGGGAGAAAGGCGCCGTCGCTCCCGTTAAAAACCAGCAGAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCAGCTGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTCATAGCCTTATCAGAACAGGAGCTGGTGGATTGTGACACTACTTATAACGAAGGTTGCAATGGAGGGTTAATGGACTATGCTTTTGAATTCATCATCAACAATGGCGGCATTGACACTGAAGATGATTATCCTTACAAAGGTTTTGATAGCACCTGCATCCAATATAAGACCTCCCTGTTAACAATGAGAAAGCACTTCAAAAAGCAACCAGCTAAACAGGTTGTGGCTGTTGCCATTCAAGGAGGTGGTAGAAGTTTCCAGCTATATCATTCGGGTATATTCACAAGAATATGTGGAACAGAATTAGACCATGGTGTTAACGTGGTTGGCTACGGGTCAGAAGGGGGCATTGATTACTGGATTGTAAGAAACTCATGGGGAGCAAGTTGGGGCAAACATGGCTACATCAGGATGCAACGCAACGTGGGTTCATCAAAAGGTCTATGTGGGATTGCAATGCAGCCTTCCTACCCCATTAAAAACGGACCAAACCCTCCAAATCCCGGCCCAACTCCTCCGAGTCCGGTGAAGCCGCCTTCGGTATGCGATGAATATTATACCTGTCCTGCATCTAAAACTTGCTGCTGCATTTTTGAGTTCTCAAAGTTGTGCTTTCAATGGGGATGCTGCCCTCTTGAAAGCGCCACATGTTGTGATGATCATGAGAGCTGCTGCCCCCATGAGTACCCTATCTGTAACGTCTGTGCTGGAACTTGCTTGAAGGGAAAGAACGATGGTTTTGGAGTGAAGGCGATGAGGCGCAGTGTAGCTGCAGCTAAACCATCGTGGGCATTTGGAGATACGACTATCGGAAAGAGTAGTGCTTAA

Coding sequence (CDS)

ATGGAAACCTACAAATACAAATCCTCGCCGATTATTGCCTTCCTCCTCTTCCTCACAATAACTACTACATCATCGGCGTTGGAATCTCCGATCATCCCGGCGAGGACGGACGATGAAGTGACGGCGCTGTACGACGACTGGCGCGCCAAGCACGGCAAGTTGCAAAACGGCTTGGATGATCGATTCCAGATATTTAAAGACAATCTCAAATACATCGACGAAATCAACGCCCTAGATTTGCCCTACCGGTTGGGGTTGAATGTTTTCGCAGATCTGACGAACGAGGAGTACCGGTCCAGGAATTTGGGAACCAAGTTCGCGGCCGGTTCGAGGCGGAACAAGACCAGCAGCCGGTATCTGCCTCGGCTTGGGGATGAGCTGCCCGGCTCGGTCGATTGGCGGGAGAAAGGCGCCGTCGCTCCCGTTAAAAACCAGCAGAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCAGCTGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTCATAGCCTTATCAGAACAGGAGCTGGTGGATTGTGACACTACTTATAACGAAGGTTGCAATGGAGGGTTAATGGACTATGCTTTTGAATTCATCATCAACAATGGCGGCATTGACACTGAAGATGATTATCCTTACAAAGGTTTTGATAGCACCTGCATCCAATATAAGACCTCCCTGTTAACAATGAGAAAGCACTTCAAAAAGCAACCAGCTAAACAGGTTGTGGCTGTTGCCATTCAAGGAGGTGGTAGAAGTTTCCAGCTATATCATTCGGGTATATTCACAAGAATATGTGGAACAGAATTAGACCATGGTGTTAACGTGGTTGGCTACGGGTCAGAAGGGGGCATTGATTACTGGATTGTAAGAAACTCATGGGGAGCAAGTTGGGGCAAACATGGCTACATCAGGATGCAACGCAACGTGGGTTCATCAAAAGGTCTATGTGGGATTGCAATGCAGCCTTCCTACCCCATTAAAAACGGACCAAACCCTCCAAATCCCGGCCCAACTCCTCCGAGTCCGGTGAAGCCGCCTTCGGTATGCGATGAATATTATACCTGTCCTGCATCTAAAACTTGCTGCTGCATTTTTGAGTTCTCAAAGTTGTGCTTTCAATGGGGATGCTGCCCTCTTGAAAGCGCCACATGTTGTGATGATCATGAGAGCTGCTGCCCCCATGAGTACCCTATCTGTAACGTCTGTGCTGGAACTTGCTTGAAGGGAAAGAACGATGGTTTTGGAGTGAAGGCGATGAGGCGCAGTGTAGCTGCAGCTAAACCATCGTGGGCATTTGGAGATACGACTATCGGAAAGAGTAGTGCTTAA

Protein sequence

METYKYKSSPIIAFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGLDDRFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTMRKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGTCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA
Homology
BLAST of Sed0013090 vs. NCBI nr
Match: XP_022982248.1 (low-temperature-induced cysteine proteinase-like [Cucurbita maxima])

HSP 1 Score: 750.7 bits (1937), Expect = 7.0e-213
Identity = 361/458 (78.82%), Postives = 395/458 (86.24%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ AL S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPESVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ SS GLCGIAM+PSYPIK GPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIASSTGLCGIAMEPSYPIKKGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD ++GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVSVGKSSA 461

BLAST of Sed0013090 vs. NCBI nr
Match: XP_022940939.1 (low-temperature-induced cysteine proteinase-like [Cucurbita moschata])

HSP 1 Score: 749.2 bits (1933), Expect = 2.0e-212
Identity = 360/458 (78.60%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ AL S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ S  GLCGIAM+PSYP KNGPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIDSKTGLCGIAMEPSYPTKNGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD  +GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Sed0013090 vs. NCBI nr
Match: XP_038897503.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 747.7 bits (1929), Expect = 5.9e-212
Identity = 361/457 (78.99%), Postives = 394/457 (86.21%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DDR 65
           Y SSPI+A L FL I   SSA  S IIP RTD+EV ALYD WRAKHGKL+N L    ++R
Sbjct: 4   YLSSPIMALLFFLFI-ALSSASPSSIIPERTDEEVMALYDQWRAKHGKLRNNLGAEPENR 63

Query: 66  FQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLP 125
           F IFKDNLKYIDEINA +LPYRLGLNVFADLTNEEYRSR LG KFAAGSRRN+TSSRYLP
Sbjct: 64  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFAAGSRRNRTSSRYLP 123

Query: 126 RLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVD 185
           RLGDE+P S+DWREKGAVAPVK+Q SCGSCWAFSTIAAVEGINQIVTGD IALSEQELVD
Sbjct: 124 RLGDEIPDSIDWREKGAVAPVKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQELVD 183

Query: 186 CDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSL----------- 245
           CD +YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +            
Sbjct: 184 CDKSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYEDV 243

Query: 246 -LTMRKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGID 305
            +   K  +K  +KQVVAVAI+GGGRSFQLY SGIFT  CGT+LDHGVNVVGYGSEGG+D
Sbjct: 244 PINNEKALQKAASKQVVAVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVD 303

Query: 306 YWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKP 365
           YWIVRNSWGASWG+ GYIRMQRN+ SS GLCGIAM+PSYP K+GPNPPNPGPTPPSPVKP
Sbjct: 304 YWIVRNSWGASWGESGYIRMQRNIASSTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKP 363

Query: 366 PSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGT 425
           PSVCDEYYTCPA++TCCC+FEFS +C +WGCCPLESATCCDDH SCCPH+YPICNV AGT
Sbjct: 364 PSVCDEYYTCPAAETCCCVFEFSTMCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGT 423

Query: 426 CLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           CLKGKND FGVKAMRR+ AAA+PSWA GD T+GKSSA
Sbjct: 424 CLKGKNDVFGVKAMRRTTAAARPSWAHGDVTVGKSSA 459

BLAST of Sed0013090 vs. NCBI nr
Match: KAG6608453.1 (Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037789.1 Cysteine proteinase RD21A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 747.7 bits (1929), Expect = 5.9e-212
Identity = 359/458 (78.38%), Postives = 392/458 (85.59%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ A  S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVVITLSSALAFASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ S  GLCGIAM+PSYP KNGPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIDSPTGLCGIAMEPSYPTKNGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD  +GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Sed0013090 vs. NCBI nr
Match: XP_023524911.1 (low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.3 bits (1928), Expect = 7.8e-212
Identity = 359/458 (78.38%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ AL S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ S  GLCGIAM+PSYP KNGPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIDSPTGLCGIAMEPSYPTKNGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH++PICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDFPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD  +GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Sed0013090 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.6e-159
Identity = 276/455 (60.66%), Postives = 328/455 (72.09%), Query Frame = 0

Query: 15  LLFLTITTTSSALESPIIP-----------ARTDDEVTALYDDWRAKHGKL--QNGL--- 74
           +LFL +   SSA++  II             R++ EV ++Y+ W  KHGK   QN L   
Sbjct: 10  ILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEK 69

Query: 75  DDRFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSR 134
           D RF+IFKDNL+++DE N  +L YRLGL  FADLTN+EYRS+ LG K      R +TS R
Sbjct: 70  DRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-RTSLR 129

Query: 135 YLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQE 194
           Y  R+GDELP S+DWR+KGAVA VK+Q  CGSCWAFSTI AVEGINQIVTGD I LSEQE
Sbjct: 130 YEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQE 189

Query: 195 LVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM----- 254
           LVDCDT+YNEGCNGGLMDYAFEFII NGGIDT+ DYPYKG D TC Q + +   +     
Sbjct: 190 LVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDSY 249

Query: 255 -------RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEG 314
                   +  KK  A Q +++AI+ GGR+FQLY SGIF   CGT+LDHGV  VGYG+E 
Sbjct: 250 EDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGYGTEN 309

Query: 315 GIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSP 374
           G DYWIVRNSWG SWG+ GY+RM RN+ SS G CGIA++PSYPIKNG NPPNPGP+PPSP
Sbjct: 310 GKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPGPSPPSP 369

Query: 375 VKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVC 434
           +KPP+ CD YYTCP S TCCC+FE+ K CF WGCCPLE+ATCCDD+ SCCPHEYP+C++ 
Sbjct: 370 IKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYPVCDLD 429

Query: 435 AGTCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTI 442
            GTCL  KN  F VKA++R    A P W+ G   I
Sbjct: 430 QGTCLLSKNSPFSVKALKRK--PATPFWSQGRKNI 461

BLAST of Sed0013090 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-152
Identity = 272/459 (59.26%), Postives = 328/459 (71.46%), Query Frame = 0

Query: 7   KSSPIIAFLLFLTITTTSSALESPII-----------PARTDDEVTALYDDWRAKHGKL- 66
           K SP+I   L L +   S A++  II            +R+D EV  +Y+ W  +HGK  
Sbjct: 5   KLSPMI---LLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKK 64

Query: 67  --QNGL----DDRFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAA 126
             QNGL    D RF+IFKDNL++IDE N  +L Y+LGL  FADLTNEEYRS  LG K   
Sbjct: 65  MNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK--P 124

Query: 127 GSRRNKTSSRYLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVT 186
             R  KTS RY  R+GD LP SVDWR++GAVA VK+Q SCGSCWAFSTI AVEGIN+IVT
Sbjct: 125 TKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVT 184

Query: 187 GDFIALSEQELVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYK- 246
           GD I+LSEQELVDCDT+YN+GCNGGLMDYAFEFII NGGIDTE DYPYK  D  C Q + 
Sbjct: 185 GDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRK 244

Query: 247 -TSLLTMRKH----------FKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHG 306
              ++T+  +           KK  A Q ++VAI+ GGR+FQLY SG+F  +CGTELDHG
Sbjct: 245 NAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHG 304

Query: 307 VNVVGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNP 366
           V  VGYG+E G DYWIVRNSWG  WG+ GYI+M RN+ +  G CGIAM+ SYPIK G NP
Sbjct: 305 VVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKGQNP 364

Query: 367 PNPGPTPPSPVKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCC 426
           PNPGP+PPSP+KPP+ CD+Y++CP S TCCC++++ K CF WGCCPLE+ATCCDD+ SCC
Sbjct: 365 PNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNSSCC 424

Query: 427 PHEYPICNVCAGTCLKGKNDGFGVKAMRRSVAAAKPSWA 436
           PHEYP+C+V  GTCL  KN  F VKA++R+ A   P WA
Sbjct: 425 PHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Sed0013090 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 538.9 bits (1387), Expect = 5.5e-152
Identity = 269/453 (59.38%), Postives = 318/453 (70.20%), Query Frame = 0

Query: 13  AFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGLDD---RFQIFKDNL 72
           A LL L++     ++ S     R+++E   LY +W+A+HGK  N + +   R+  F+DNL
Sbjct: 11  ALLLLLSLAAADMSIVS--YGERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNL 70

Query: 73  KYIDEINALD----LPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLPRLGD 132
           +YIDE NA        +RLGLN FADLTNEEYR   LG +     R  K S RYL    +
Sbjct: 71  RYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLR-NKPRRERKVSDRYLAADNE 130

Query: 133 ELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVDCDTT 192
            LP SVDWR KGAVA +K+Q  CGSCWAFS IAAVEGINQIVTGD I+LSEQELVDCDT+
Sbjct: 131 ALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTS 190

Query: 193 YNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTC------------IQYKTSLLTM 252
           YNEGCNGGLMDYAF+FIINNGGIDTEDDYPYKG D  C              Y+      
Sbjct: 191 YNEGCNGGLMDYAFDFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNS 250

Query: 253 RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGIDYWIV 312
               +K  A Q V+VAI+ GGR+FQLY SGIFT  CGT LDHGV  VGYG+E G DYWIV
Sbjct: 251 ETSLQKAVANQPVSVAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIV 310

Query: 313 RNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKPPSVC 372
           RNSWG SWG+ GY+RM+RN+ +S G CGIA++PSYP+K G NPPNPGPTPPSP  PP+VC
Sbjct: 311 RNSWGKSWGESGYVRMERNIKASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVC 370

Query: 373 DEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGTCLKG 432
           D YYTCP S TCCCI+E+ K C+ WGCCPLE ATCCDDH SCCPHEYPICNV  GTCL  
Sbjct: 371 DNYYTCPDSTTCCCIYEYGKYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMA 430

Query: 433 KNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           K+    VKA++R++A    S+ FG+   GK S+
Sbjct: 431 KDSPLAVKALKRTLAKPNLSFLFGN---GKKSS 457

BLAST of Sed0013090 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 458.0 bits (1177), Expect = 1.2e-127
Identity = 239/455 (52.53%), Postives = 288/455 (63.30%), Query Frame = 0

Query: 13  AFLLFLTITTTSSALESPII------------PARTDDEVTALYDDWRAKHG-----KLQ 72
           AFLL L +   ++A +  II               T+ E  A YD W A++G      L 
Sbjct: 9   AFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGPTEAEARAAYDLWLAENGGGSPNALG 68

Query: 73  NGLDDRFQIFKDNLKYIDEINALDLP---YRLGLNVFADLTNEEYRSRNLGTKFAAGSRR 132
              + RF +F DNLK++D  NA       +RLG+N FADLTNEE+R+  LG K A  SR 
Sbjct: 69  GEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATFLGAKVAERSR- 128

Query: 133 NKTSSRYLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFI 192
                RY     +ELP SVDWREKGAVAPVKNQ  CGSCWAFS ++ VE INQ+VTG+ I
Sbjct: 129 -AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESINQLVTGEMI 188

Query: 193 ALSEQELVDCDTT-YNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLL 252
            LSEQELV+C T   N GCNGGLMD AF+FII NGGIDTEDDYPYK  D  C   + +  
Sbjct: 189 TLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGKCDINRENAK 248

Query: 253 TM------------RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNV 312
            +             K  +K  A Q V+VAI+ GGR FQLYHSG+F+  CGT LDHGV  
Sbjct: 249 VVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCGTSLDHGVVA 308

Query: 313 VGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNP 372
           VGYG++ G DYWIVRNSWG  WG+ GY+RM+RN+  + G CGIAM  SYP K+G NPP P
Sbjct: 309 VGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCGIAMMASYPTKSGANPPKP 368

Query: 373 GPTPPSPVKPP------SVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHE 429
            PTPP+P  PP       VCD+ ++CPA  TCCC F F  LC  WGCCP+E ATCC DH 
Sbjct: 369 SPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPVEGATCCKDHA 428

BLAST of Sed0013090 vs. ExPASy Swiss-Prot
Match: P20721 (Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.7e-124
Identity = 210/347 (60.52%), Postives = 259/347 (74.64%), Query Frame = 0

Query: 112 RNKTSSRYLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDF 171
           +NK S RYLP++GD LP S+DWREKG +  VK+Q SCGSCWAFS +AA+E IN IVTG+ 
Sbjct: 4   KNK-SDRYLPKVGDSLPESIDWREKGVLVGVKDQGSCGSCWAFSAVAAMESINAIVTGNL 63

Query: 172 IALSEQELVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLL 231
           I+LSEQELVDCD +YNEGC+GGLMDYAFEF+I NGGIDTE+DYPYK  +  C QY+ +  
Sbjct: 64  ISLSEQELVDCDRSYNEGCDGGLMDYAFEFVIKNGGIDTEEDYPYKERNGVCDQYRKNAK 123

Query: 232 TMR------------KHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNV 291
            ++            K  +K  A Q V++A++ GGR FQ Y SGIFT  CGT +DHGV +
Sbjct: 124 VVKIDSYEDVPVNNEKALQKAVAHQPVSIALEAGGRDFQHYKSGIFTGKCGTAVDHGVVI 183

Query: 292 VGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNP 351
            GYG+E G+DYWIVRNSWGA+  ++GY+R+QRNV SS GLCG+A++PSYP+K GPNPP P
Sbjct: 184 AGYGTENGMDYWIVRNSWGANCRENGYLRVQRNVSSSSGLCGLAIEPSYPVKTGPNPPKP 243

Query: 352 GPTPPSPVKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHE 411
            P+PPSPVKPP+ CDEY  C    TCCCI +F + CF WGCCPLE ATCC+DH SCCPH+
Sbjct: 244 APSPPSPVKPPTECDEYSQCAVGTTCCCILQFRRSCFSWGCCPLEGATCCEDHYSCCPHD 303

Query: 412 YPICNVCAGTCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           YPICNV  GTC   K +  GVKAM+R +  A+P  AFG+   GK S+
Sbjct: 304 YPICNVRQGTCSMSKGNPLGVKAMKRIL--AQPIGAFGNG--GKKSS 345

BLAST of Sed0013090 vs. ExPASy TrEMBL
Match: A0A6J1J4D3 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111481130 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.4e-213
Identity = 361/458 (78.82%), Postives = 395/458 (86.24%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ AL S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPESVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ SS GLCGIAM+PSYPIK GPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIASSTGLCGIAMEPSYPIKKGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD ++GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVSVGKSSA 461

BLAST of Sed0013090 vs. ExPASy TrEMBL
Match: A0A6J1FLR7 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111446375 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 9.9e-213
Identity = 360/458 (78.60%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSS-ALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DD 65
           Y+SS I+  L F+ IT +S+ AL S II  RTD+EV ALYD WRAKHGKL+NGL    DD
Sbjct: 4   YRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRDD 63

Query: 66  RFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYL 125
           RF+IFKDNLKYIDEINA +LPYRLGLNVFADLTN EYRS+ LGT+FAAGSRRNKTSSRY 
Sbjct: 64  RFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRYQ 123

Query: 126 PRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELV 185
           PRLGD+LP SVDWREKGAV PVK+Q SCGSCWAFSTIAAVEGINQ+VTGD ++LSEQELV
Sbjct: 124 PRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQELV 183

Query: 186 DCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM------- 245
           DCDT+YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +   +       
Sbjct: 184 DCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYED 243

Query: 246 -----RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGI 305
                 K  +K  A QVVAVAI+GGGRSFQLY SGIFT  CGT LDHGVNVVGYGSEGG+
Sbjct: 244 VPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGGV 303

Query: 306 DYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVK 365
           DYWIVRNSWG SWG+HGYIRMQRN+ S  GLCGIAM+PSYP KNGPNPPNPGPTPPSPVK
Sbjct: 304 DYWIVRNSWGGSWGEHGYIRMQRNIDSKTGLCGIAMEPSYPTKNGPNPPNPGPTPPSPVK 363

Query: 366 PPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAG 425
           PPSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AG
Sbjct: 364 PPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAG 423

Query: 426 TCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           TCLKGKND FGVKAMRR+ AAAKPSWAFGD  +GKSSA
Sbjct: 424 TCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Sed0013090 vs. ExPASy TrEMBL
Match: A0A6J1C3C7 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111008097 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.2e-205
Identity = 351/457 (76.81%), Postives = 387/457 (84.68%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DDR 65
           Y+SSPI+  L F+ I T SSA++  II  RTD+EV ALY +W AKHGK  NGL    ++R
Sbjct: 4   YRSSPIMLLLFFIAI-TLSSAMDYSIISGRTDEEVMALYREWCAKHGKQHNGLGAERENR 63

Query: 66  FQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLP 125
           F+IFKDNLKYIDEIN+ +LPYRLGLNVFADLTN+EYRS+ LG K AAGSRRNKTSSRY P
Sbjct: 64  FEIFKDNLKYIDEINSQNLPYRLGLNVFADLTNDEYRSKYLGPKRAAGSRRNKTSSRYHP 123

Query: 126 RLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVD 185
           RLGD LP S+DWREKGAV  VK+Q SCGSCWAFSTIAAVEGINQIVTGD I+LSEQELVD
Sbjct: 124 RLGDNLPDSIDWREKGAVVGVKDQGSCGSCWAFSTIAAVEGINQIVTGDLISLSEQELVD 183

Query: 186 CDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSL----------- 245
           CD +YNEGCNGGLMDYAFEFIINNGGIDTE+DYPY GFDS+CIQYK +            
Sbjct: 184 CDNSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNAKVVSIDDYEDV 243

Query: 246 -LTMRKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGID 305
            +   K  +K  A QVVAVAI+GGGRS+QLY SGIFT  CGT LDHGVNVVGYGSEGG+D
Sbjct: 244 PVNNEKALQKAVANQVVAVAIEGGGRSYQLYESGIFTGRCGTALDHGVNVVGYGSEGGVD 303

Query: 306 YWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKP 365
           YWIVRNSWG SWG+ GYIRMQRNV S  GLCGIAM+PSYPIKNGPNPPNPGPTPPSPVKP
Sbjct: 304 YWIVRNSWGKSWGEKGYIRMQRNVPSKNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPVKP 363

Query: 366 PSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGT 425
           PSVCDEYY+CPAS+TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AGT
Sbjct: 364 PSVCDEYYSCPASETCCCIFEFSNLCVEWGCCPLESATCCDDHYSCCPHDYPICNVRAGT 423

Query: 426 CLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           CL+GKND FGVKAMRR+ AAAKPSWAFGD ++GKSSA
Sbjct: 424 CLRGKNDPFGVKAMRRTAAAAKPSWAFGDASVGKSSA 459

BLAST of Sed0013090 vs. ExPASy TrEMBL
Match: A0A1S3BV04 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103493570 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.4e-204
Identity = 346/457 (75.71%), Postives = 386/457 (84.46%), Query Frame = 0

Query: 6   YKSSPIIAFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DDR 65
           ++SS  +A L FL I   S+A  S IIP RTD+EV ALYD WRA HGKL N L    ++R
Sbjct: 4   FQSSQTMALLFFLFI-ALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENR 63

Query: 66  FQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLP 125
           F IFKDNLKYIDEINA +LPYRLGLNVFADLTNEEYRSR LG +FAAGSRRN+TS+RYLP
Sbjct: 64  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLP 123

Query: 126 RLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVD 185
           RLGDELP S+DWR KGAVAPVK+Q SCGSCWAFST+A+VEGINQIVTGD IALSEQELVD
Sbjct: 124 RLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVD 183

Query: 186 CDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSL----------- 245
           CD +YNEGCNGGLMDYAFEFIINNGG+DTE+DYPY GFDS+CIQYK +            
Sbjct: 184 CDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDV 243

Query: 246 -LTMRKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGID 305
            +   K  +K  +KQVV+VAI+GGGRSFQLY SGIFT  CGT+LDHGVNVVGYGSEGG+D
Sbjct: 244 PVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVD 303

Query: 306 YWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKP 365
           YWIVRNSWGA+WG+ GY+RMQRN+ S  GLCGIAM+PSYP K+GPNPPNPGPTPPSPVKP
Sbjct: 304 YWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKP 363

Query: 366 PSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGT 425
           PSVCDEYYTCPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AGT
Sbjct: 364 PSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGT 423

Query: 426 CLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           C K KND FGVKAMRR+ AAA+PSWA  D T+GKSSA
Sbjct: 424 CSKSKNDIFGVKAMRRTAAAARPSWAHRDVTVGKSSA 459

BLAST of Sed0013090 vs. ExPASy TrEMBL
Match: A0A5A7VFD6 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003080 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.4e-203
Identity = 342/448 (76.34%), Postives = 379/448 (84.60%), Query Frame = 0

Query: 15  LLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGL----DDRFQIFKDNLK 74
           LLF      S+A  S IIP RTD+EV ALYD WRA HGKL N L    ++RF IFKDNLK
Sbjct: 3   LLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENRFHIFKDNLK 62

Query: 75  YIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLPRLGDELPGS 134
           YIDEINA +LPYRLGLNVFADLTNEEYRSR LG +FAAGSRRN+TS+RYLPRLGDELP S
Sbjct: 63  YIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLPRLGDELPDS 122

Query: 135 VDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVDCDTTYNEGC 194
           +DWR KGAVAPVK+Q SCGSCWAFST+A+VEGINQIVTGD IALSEQELVDCD +YNEGC
Sbjct: 123 IDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVDCDKSYNEGC 182

Query: 195 NGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSL------------LTMRKHFK 254
           NGGLMDYAFEFIINNGG+DTE+DYPY GFDS+CIQYK +             +   K  +
Sbjct: 183 NGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDVPVNNEKALQ 242

Query: 255 KQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGIDYWIVRNSWG 314
           K  +KQVV+VAI+GGGRSFQLY SGIFT  CGT+LDHGVNVVGYGSEGG+DYWIVRNSWG
Sbjct: 243 KAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNSWG 302

Query: 315 ASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKPPSVCDEYYT 374
           A+WG+ GY+RMQRN+ S  GLCGIAM+PSYP K+GPNPPNPGPTPPSPVKPPSVCDEYYT
Sbjct: 303 ANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKPPSVCDEYYT 362

Query: 375 CPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGTCLKGKNDGF 434
           CPA++TCCCIFEFS LC +WGCCPLESATCCDDH SCCPH+YPICNV AGTC K KND F
Sbjct: 363 CPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKNDIF 422

Query: 435 GVKAMRRSVAAAKPSWAFGDTTIGKSSA 447
           GVKAMRR+ AAA+PSWA  D T+GKSSA
Sbjct: 423 GVKAMRRTAAAARPSWAHRDVTVGKSSA 450

BLAST of Sed0013090 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 563.9 bits (1452), Expect = 1.1e-160
Identity = 276/455 (60.66%), Postives = 328/455 (72.09%), Query Frame = 0

Query: 15  LLFLTITTTSSALESPIIP-----------ARTDDEVTALYDDWRAKHGKL--QNGL--- 74
           +LFL +   SSA++  II             R++ EV ++Y+ W  KHGK   QN L   
Sbjct: 10  ILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEK 69

Query: 75  DDRFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSR 134
           D RF+IFKDNL+++DE N  +L YRLGL  FADLTN+EYRS+ LG K      R +TS R
Sbjct: 70  DRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-RTSLR 129

Query: 135 YLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQE 194
           Y  R+GDELP S+DWR+KGAVA VK+Q  CGSCWAFSTI AVEGINQIVTGD I LSEQE
Sbjct: 130 YEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQE 189

Query: 195 LVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYKTSLLTM----- 254
           LVDCDT+YNEGCNGGLMDYAFEFII NGGIDT+ DYPYKG D TC Q + +   +     
Sbjct: 190 LVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDSY 249

Query: 255 -------RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEG 314
                   +  KK  A Q +++AI+ GGR+FQLY SGIF   CGT+LDHGV  VGYG+E 
Sbjct: 250 EDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGYGTEN 309

Query: 315 GIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSP 374
           G DYWIVRNSWG SWG+ GY+RM RN+ SS G CGIA++PSYPIKNG NPPNPGP+PPSP
Sbjct: 310 GKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPGPSPPSP 369

Query: 375 VKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVC 434
           +KPP+ CD YYTCP S TCCC+FE+ K CF WGCCPLE+ATCCDD+ SCCPHEYP+C++ 
Sbjct: 370 IKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYPVCDLD 429

Query: 435 AGTCLKGKNDGFGVKAMRRSVAAAKPSWAFGDTTI 442
            GTCL  KN  F VKA++R    A P W+ G   I
Sbjct: 430 QGTCLLSKNSPFSVKALKRK--PATPFWSQGRKNI 461

BLAST of Sed0013090 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.0e-153
Identity = 272/459 (59.26%), Postives = 328/459 (71.46%), Query Frame = 0

Query: 7   KSSPIIAFLLFLTITTTSSALESPII-----------PARTDDEVTALYDDWRAKHGKL- 66
           K SP+I   L L +   S A++  II            +R+D EV  +Y+ W  +HGK  
Sbjct: 5   KLSPMI---LLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKK 64

Query: 67  --QNGL----DDRFQIFKDNLKYIDEINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAA 126
             QNGL    D RF+IFKDNL++IDE N  +L Y+LGL  FADLTNEEYRS  LG K   
Sbjct: 65  MNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK--P 124

Query: 127 GSRRNKTSSRYLPRLGDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVT 186
             R  KTS RY  R+GD LP SVDWR++GAVA VK+Q SCGSCWAFSTI AVEGIN+IVT
Sbjct: 125 TKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVT 184

Query: 187 GDFIALSEQELVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQYK- 246
           GD I+LSEQELVDCDT+YN+GCNGGLMDYAFEFII NGGIDTE DYPYK  D  C Q + 
Sbjct: 185 GDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRK 244

Query: 247 -TSLLTMRKH----------FKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHG 306
              ++T+  +           KK  A Q ++VAI+ GGR+FQLY SG+F  +CGTELDHG
Sbjct: 245 NAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHG 304

Query: 307 VNVVGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNP 366
           V  VGYG+E G DYWIVRNSWG  WG+ GYI+M RN+ +  G CGIAM+ SYPIK G NP
Sbjct: 305 VVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKGQNP 364

Query: 367 PNPGPTPPSPVKPPSVCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCC 426
           PNPGP+PPSP+KPP+ CD+Y++CP S TCCC++++ K CF WGCCPLE+ATCCDD+ SCC
Sbjct: 365 PNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNSSCC 424

Query: 427 PHEYPICNVCAGTCLKGKNDGFGVKAMRRSVAAAKPSWA 436
           PHEYP+C+V  GTCL  KN  F VKA++R+ A   P WA
Sbjct: 425 PHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Sed0013090 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 445.3 bits (1144), Expect = 5.9e-125
Identity = 228/436 (52.29%), Postives = 292/436 (66.97%), Query Frame = 0

Query: 15  LLFLTITTTSSALESPIIPARTDDEVTA--LYDDWRAKHGKLQNGLDD---RFQIFKDNL 74
           LL  ++   S +L S      T +E  A  +Y+ W  ++ K  NGL +   RF+IFKDNL
Sbjct: 12  LLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNL 71

Query: 75  KYIDEINAL-DLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLPRLGDELP 134
           K+++E +++ +  Y +GL  FADLTN+E+R+  L +K    +R      +YL ++GD LP
Sbjct: 72  KFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER-TRVPVKGEKYLYKVGDSLP 131

Query: 135 GSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVDCDTTYNE 194
            ++DWR KGAV PVK+Q SCGSCWAFS I AVEGINQI TG+ I+LSEQELVDCDT+YN+
Sbjct: 132 DAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYND 191

Query: 195 GCNGGLMDYAFEFIINNGGIDTEDDYPYKGFD-STCIQYK--TSLLTM----------RK 254
           GC GGLMDYAF+FII NGGIDTE+DYPY   D + C   K  T ++T+           K
Sbjct: 192 GCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEK 251

Query: 255 HFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGIDYWIVRN 314
             KK  A Q ++VAI+ GGR+FQLY SG+FT  CGT LDHGV  VGYGSEGG DYWIVRN
Sbjct: 252 SLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRN 311

Query: 315 SWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIK-NGPNPPNPGPTPPSPVKPPSVCD 374
           SWG++WG+ GY +++RN+  S G CG+AM  SYP K +G NPP P      P   P VCD
Sbjct: 312 SWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPPKP------PAPSPVVCD 371

Query: 375 EYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGTCLKGK 431
           +  TCPA  TCCC++E++  C+ WGCCP ESATCCDD  SCCP  YP+C++ A TC    
Sbjct: 372 KSNTCPAKSTCCCLYEYNGKCYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKG 431

BLAST of Sed0013090 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 393.3 bits (1009), Expect = 2.6e-109
Identity = 210/443 (47.40%), Postives = 270/443 (60.95%), Query Frame = 0

Query: 8   SSPIIAFLLFLTITTTSSALESPIIPARTDDEVTALYDDWRAKHGKLQNGLDD---RFQI 67
           SS  I+   F  +  +SS+         + D+++ L+DDW  KHGK     ++   R QI
Sbjct: 5   SSSFISLTFFFLLLVSSSS---------SSDDISELFDDWCQKHGKTYGSEEERQQRIQI 64

Query: 68  FKDNLKYIDEINAL-DLPYRLGLNVFADLTNEEYRSRNLGTKFAAGSRRNKTSSRYLPRL 127
           FKDN  ++ + N + +  Y L LN FADLT+ E+++  LG   +A S    +  + L   
Sbjct: 65  FKDNHDFVTQHNLITNATYSLSLNAFADLTHHEFKASRLGLSVSAPSVIMASKGQSLGG- 124

Query: 128 GDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQIVTGDFIALSEQELVDCD 187
             ++P SVDWR+KGAV  VK+Q SCG+CW+FS   A+EGINQIVTGD I+LSEQEL+DCD
Sbjct: 125 SVKVPDSVDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGDLISLSEQELIDCD 184

Query: 188 TTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTC--IQYKTSLLTM-------- 247
            +YN GCNGGLMDYAFEF+I N GIDTE DYPY+  D TC   + K  ++T+        
Sbjct: 185 KSYNAGCNGGLMDYAFEFVIKNHGIDTEKDYPYQERDGTCKKDKLKQKVVTIDSYAGVKS 244

Query: 248 --RKHFKKQPAKQVVAVAIQGGGRSFQLYHSGIFTRICGTELDHGVNVVGYGSEGGIDYW 307
              K   +  A Q V+V I G  R+FQLY SGIF+  C T LDH V +VGYGS+ G+DYW
Sbjct: 245 NDEKALMEAVAAQPVSVGICGSERAFQLYSSGIFSGPCSTSLDHAVLIVGYGSQNGVDYW 304

Query: 308 IVRNSWGASWGKHGYIRMQRNVGSSKGLCGIAMQPSYPIKNGPNPPNPGPTPPSPVKPPS 367
           IV+NSWG SWG  G++ MQRN  +S G+CGI M  SYPIK  PNP  P P+PP P K   
Sbjct: 305 IVKNSWGKSWGMDGFMHMQRNTENSDGVCGINMLASYPIKTHPNP--PPPSPPGPTK--- 364

Query: 368 VCDEYYTCPASKTCCCIFEFSKLCFQWGCCPLESATCCDDHESCCPHEYPICNVCAGTCL 427
            C+ +  C + +TCCC  E   LCF W CC +ESA CC D   CCPH+YP+C+     CL
Sbjct: 365 -CNLFTYCSSGETCCCARELFGLCFSWKCCEIESAVCCKDGRHCCPHDYPVCDTTRSLCL 421

Query: 428 KGKNDGFGVKAMRRSVAAAKPSW 435
           K K   F          A KP W
Sbjct: 425 K-KTGNF---------TAIKPFW 421

BLAST of Sed0013090 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 351.7 bits (901), Expect = 8.8e-97
Identity = 188/364 (51.65%), Postives = 240/364 (65.93%), Query Frame = 0

Query: 8   SSPIIAFLLFLTITTTSSALESPI-----IPA----RTDDEVTALYDDWRAKHGKLQNG- 67
           S+ +++ LL   + + +S  ES I     +P+    RTD+EV ++Y  W A+HGK  N  
Sbjct: 4   STKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNN 63

Query: 68  ------LDDRFQIFKDNLKYID--EINALDLPYRLGLNVFADLTNEEYRSRNLGTKFAAG 127
                  D RF IFKDNL++ID    N  +  Y+LGL  F DLTN+EYR   LG +    
Sbjct: 64  NGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPA 123

Query: 128 SRRNK---TSSRYLPRL-GDELPGSVDWREKGAVAPVKNQQSCGSCWAFSTIAAVEGINQ 187
            R  K    + +Y   + G E+P +VDWR+KGAV P+K+Q +CGSCWAFST AAVEGIN+
Sbjct: 124 RRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINK 183

Query: 188 IVTGDFIALSEQELVDCDTTYNEGCNGGLMDYAFEFIINNGGIDTEDDYPYKGFDSTCIQ 247
           IVTG+ I+LSEQELVDCD +YN+GCNGGLMDYAF+FI+ NGG++TE DYPY+GF   C  
Sbjct: 184 IVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNS 243

Query: 248 Y-KTSLLTMRKHFKKQPAK-----------QVVAVAIQGGGRSFQLYHSGIFTRICGTEL 307
           + K S +     ++  P K           Q V+VAI+ GGR FQ Y SGIFT  CGT L
Sbjct: 244 FLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNL 303

Query: 308 DHGVNVVGYGSEGGIDYWIVRNSWGASWGKHGYIRMQRNVGSSK-GLCGIAMQPSYPIKN 337
           DH V  VGYGSE G+DYWIVRNSWG  WG+ GYIRM+RN+ +SK G CGIA++ SYP+K 
Sbjct: 304 DHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVKY 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022982248.17.0e-21378.82low-temperature-induced cysteine proteinase-like [Cucurbita maxima][more]
XP_022940939.12.0e-21278.60low-temperature-induced cysteine proteinase-like [Cucurbita moschata][more]
XP_038897503.15.9e-21278.99low-temperature-induced cysteine proteinase-like [Benincasa hispida][more]
KAG6608453.15.9e-21278.38Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_023524911.17.8e-21278.38low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P432971.6e-15960.66Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1[more]
Q9FMH81.4e-15259.26Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S... [more]
P257765.5e-15259.38Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=... [more]
P257771.2e-12752.53Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1... [more]
P207211.7e-12460.52Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum O... [more]
Match NameE-valueIdentityDescription
A0A6J1J4D33.4e-21378.82low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1FLR79.9e-21378.60low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1C3C71.2e-20576.81low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ... [more]
A0A1S3BV046.4e-20475.71low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5A7VFD62.4e-20376.34Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT1G47128.11.1e-16060.66Granulin repeat cysteine protease family protein [more]
AT5G43060.11.0e-15359.26Granulin repeat cysteine protease family protein [more]
AT3G19390.15.9e-12552.29Granulin repeat cysteine protease family protein [more]
AT1G09850.12.6e-10947.40xylem bark cysteine peptidase 3 [more]
AT4G36880.18.8e-9751.65cysteine proteinase1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 275..285
score: 56.8
coord: 145..160
score: 61.39
coord: 290..296
score: 73.06
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 127..330
e-value: 3.6E-102
score: 355.4
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 127..330
e-value: 3.2E-69
score: 233.4
IPR000118GranulinSMARTSM00277GRAN_2coord: 353..410
e-value: 8.5E-25
score: 98.4
IPR000118GranulinPFAMPF00396Granulincoord: 364..411
e-value: 1.7E-9
score: 37.9
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 44..97
e-value: 1.4E-16
score: 71.1
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 44..97
e-value: 8.2E-9
score: 35.8
IPR037277Granulin superfamilyGENE3D2.10.25.160Granulincoord: 350..413
e-value: 8.1E-12
score: 47.3
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 21..332
e-value: 3.3E-98
score: 331.4
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 27..360
NoneNo IPR availablePANTHERPTHR12411:SF749CYSTEINE PROTEASEcoord: 27..360
NoneNo IPR availableSUPERFAMILY57277Granulin repeatcoord: 350..384
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 273..283
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 290..309
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 145..156
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 128..329
e-value: 2.56974E-93
score: 278.738
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 36..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0013090.1Sed0013090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity