Sed0012948 (gene) Chayote v1

BLAST of Sed0012948 vs. NCBI nr
Match: XP_022957996.1 (GDSL esterase/lipase At4g10955 isoform X1 [Cucurbita moschata])

HSP 1 Score: 677.2 bits (1746), Expect = 9.4e-191
Identity = 324/362 (89.50%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 66  VRQELGLQEERRGLLSVMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILER 125
           ++++LG Q E+RG+LSVM SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILER
Sbjct: 5   LQEQLGFQVEKRGILSVMASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILER 64

Query: 126 DRQERRQGSEALAPRWWEFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRY 185
           DRQE+RQGS+ALAPRWWEFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRY
Sbjct: 65  DRQEKRQGSQALAPRWWEFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRY 124

Query: 186 VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHS 245
           VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHS
Sbjct: 125 VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHS 184

Query: 246 LGSAMAMLAGKTMARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALA 305
           LGSAMAML GKTMARTGIFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA A
Sbjct: 185 LGSAMAMLTGKTMARTGIFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFA 244

Query: 306 LKAGSNSQRNRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLA 365
           LKA SN+QRN  EEPF AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLA
Sbjct: 245 LKASSNNQRNPPEEPFFAISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLA 304

Query: 366 TQNSIGGLLMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFK 425
           T+NSIGGLLMNAIGRD+EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++
Sbjct: 305 TRNSIGGLLMNAIGRDSEPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYR 364

Query: 426 YS 428
           YS
Sbjct: 365 YS 366

BLAST of Sed0012948 vs. NCBI nr
Match: XP_022996235.1 (GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996236.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996237.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996238.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_022996239.1 GDSL esterase/lipase At4g10955 [Cucurbita maxima] >XP_023534452.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534453.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534455.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534456.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo] >XP_023534457.1 GDSL esterase/lipase At4g10955-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.3 bits (1697), Expect = 4.5e-185
Identity = 315/345 (91.30%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHSLGSAMAML GKTMARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA ALKA SN+QRN  EEPF 
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLAT+NSIGGLLMNAIGRD+
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKYS 428
           EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Sed0012948 vs. NCBI nr
Match: XP_022957997.1 (GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022957998.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022957999.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata] >XP_022958000.1 GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata])

HSP 1 Score: 657.5 bits (1695), Expect = 7.7e-185
Identity = 315/345 (91.30%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHSLGSAMAML GKTMARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA ALKA SN+QRN  EEPF 
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLAT+NSIGGLLMNAIGRD+
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKYS 428
           EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Sed0012948 vs. NCBI nr
Match: KAG6605591.1 (hypothetical protein SDJN03_02908, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.1 bits (1686), Expect = 8.6e-184
Identity = 313/343 (91.25%), Postives = 327/343 (95.34%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHSLGSAMAML GKTMARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA ALKA SN+QRN  EEPF 
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLAT+NSIGGLLMNAIGRD+
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFK 426
           EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYR 343

BLAST of Sed0012948 vs. NCBI nr
Match: KAA0042666.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK06069.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 641.7 bits (1654), Expect = 4.4e-180
Identity = 308/361 (85.32%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 68  QELGLQEERRGLLSVMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDR 127
           ++LG +EERRG+L+VM SEREVFELSGPLHLA V+WENA+HRRSVAACLVQGVYILERDR
Sbjct: 18  EQLGSKEERRGILNVMASEREVFELSGPLHLASVDWENANHRRSVAACLVQGVYILERDR 77

Query: 128 QERRQGSEALAPRWWEFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVI 187
           QE+RQG+ A APRWWEFCHFRLLR L+DD DSSIFGAVYEFKP LLQG HK DGSPR+VI
Sbjct: 78  QEKRQGTAAFAPRWWEFCHFRLLRQLVDDVDSSIFGAVYEFKPLLLQGHHKVDGSPRFVI 137

Query: 188 AFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLG 247
           AFRGTLTKPDSVSRDIELDLH IQNGLHRTSRFEI MQVVRNMVATVGDSN+WLAGHSLG
Sbjct: 138 AFRGTLTKPDSVSRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNVWLAGHSLG 197

Query: 248 SAMAMLAGKTMARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALK 307
           SAMAMLAG+TMARTGIFLKSY FNPPFF+ PIERIKDKKLKHG+RIAGSVITAGLALALK
Sbjct: 198 SAMAMLAGRTMARTGIFLKSYLFNPPFFAAPIERIKDKKLKHGLRIAGSVITAGLALALK 257

Query: 308 AGS-NSQRNRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLAT 367
           AGS N+QRN  EEPF AI+AWVPSLFVNPSDH+GSEYIGYF+HRK ME++GAGSIERLAT
Sbjct: 258 AGSNNNQRNGIEEPFFAIAAWVPSLFVNPSDHVGSEYIGYFEHRKKMEDIGAGSIERLAT 317

Query: 368 QNSIGGLLMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           QNSIGGLL+NA+GR++EP+HLIPSA LV NLS AQDFKQAHGIHQWWQPH+QL S+I++Y
Sbjct: 318 QNSIGGLLLNALGRESEPIHLIPSAELVINLSPAQDFKQAHGIHQWWQPHLQLHSKIYEY 377

BLAST of Sed0012948 vs. ExPASy Swiss-Prot
Match: Q680C0 (GDSL esterase/lipase At4g10955 OS=Arabidopsis thaliana OX=3702 GN=At4g10955 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.1e-120
Identity = 210/353 (59.49%), Postives = 275/353 (77.90%), Query Frame = 0

Query: 82  VMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSE-ALAPR 141
           +MISER+ F L+GPLHL  ++W N  HRRSVA  LVQG+Y+ ERDRQ +R+G E AL+P 
Sbjct: 1   MMISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPI 60

Query: 142 WWEFCHFRLLRPLIDDADSSIFGAVYEFK--PRLLQGEHKNDGSPRYVIAFRGTLTKPDS 201
           W EF HFRL+R  +DDAD+SIFG +YE+K   +L Q     + SPR+VIAFRGT+TK DS
Sbjct: 61  WSEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDS 120

Query: 202 VSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTM 261
           +SRDIE D+H I+NGLH T+RFEI +Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+
Sbjct: 121 ISRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTI 180

Query: 262 ARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSN-SQRNRS 321
           ARTG F + + FNPPF S PIE+IKDK++KHGIRIAGSVITAGLALA KA  + SQ +R+
Sbjct: 181 ARTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRA 240

Query: 322 ----EEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGL 381
                +PF A+S W P L+VNP DH+ SEY+GYF+HR  MEE+G G +ER+ATQ+S+GG+
Sbjct: 241 LPAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGM 300

Query: 382 LMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           L+       EP+HLIPS+ L  NLS ++DFKQAHGIHQWW+   + ++++++Y
Sbjct: 301 LLGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Sed0012948 vs. ExPASy TrEMBL
Match: A0A6J1H0S6 (GDSL esterase/lipase At4g10955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459360 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 4.6e-191
Identity = 324/362 (89.50%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 66  VRQELGLQEERRGLLSVMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILER 125
           ++++LG Q E+RG+LSVM SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILER
Sbjct: 5   LQEQLGFQVEKRGILSVMASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILER 64

Query: 126 DRQERRQGSEALAPRWWEFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRY 185
           DRQE+RQGS+ALAPRWWEFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRY
Sbjct: 65  DRQEKRQGSQALAPRWWEFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRY 124

Query: 186 VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHS 245
           VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHS
Sbjct: 125 VIAFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHS 184

Query: 246 LGSAMAMLAGKTMARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALA 305
           LGSAMAML GKTMARTGIFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA A
Sbjct: 185 LGSAMAMLTGKTMARTGIFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFA 244

Query: 306 LKAGSNSQRNRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLA 365
           LKA SN+QRN  EEPF AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLA
Sbjct: 245 LKASSNNQRNPPEEPFFAISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLA 304

Query: 366 TQNSIGGLLMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFK 425
           T+NSIGGLLMNAIGRD+EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++
Sbjct: 305 TRNSIGGLLMNAIGRDSEPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYR 364

Query: 426 YS 428
           YS
Sbjct: 365 YS 366

BLAST of Sed0012948 vs. ExPASy TrEMBL
Match: A0A6J1KA98 (GDSL esterase/lipase At4g10955 OS=Cucurbita maxima OX=3661 GN=LOC111491521 PE=4 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 2.2e-185
Identity = 315/345 (91.30%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPMLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHSLGSAMAML GKTMARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA ALKA SN+QRN  EEPF 
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLAT+NSIGGLLMNAIGRD+
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKYS 428
           EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Sed0012948 vs. ExPASy TrEMBL
Match: A0A6J1H0Q1 (GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459360 PE=4 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.7e-185
Identity = 315/345 (91.30%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PVNWENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVNWENADHRRSVAACLVQGVYILERDRQEKRQGSQALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRLLRPL+DD DSSIFGAVYEFKP LLQG+HKNDGSPRYVIAFRGTLTKPDSVSRD
Sbjct: 61  EFCHFRLLRPLVDDVDSSIFGAVYEFKPVLLQGDHKNDGSPRYVIAFRGTLTKPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHFIQNGLHRTSRFEI MQVVRNMVATVGDSNLWLAGHSLGSAMAML GKTMARTG
Sbjct: 121 IELDLHFIQNGLHRTSRFEIAMQVVRNMVATVGDSNLWLAGHSLGSAMAMLTGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFLKSY FNPPFFS PIERIKDKKLKHGIRIAGSVITAGLA ALKA SN+QRN  EEPF 
Sbjct: 181 IFLKSYLFNPPFFSAPIERIKDKKLKHGIRIAGSVITAGLAFALKASSNNQRNPPEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AISAWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLAT+NSIGGLLMNAIGRD+
Sbjct: 241 AISAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATRNSIGGLLMNAIGRDS 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKYS 428
           EPLHLIPSANL+TNLS  QDFKQAHGIHQWWQPH++LQS I++YS
Sbjct: 301 EPLHLIPSANLITNLSPVQDFKQAHGIHQWWQPHLRLQSNIYRYS 345

BLAST of Sed0012948 vs. ExPASy TrEMBL
Match: A0A5A7TL26 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001700 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 2.1e-180
Identity = 308/361 (85.32%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 68  QELGLQEERRGLLSVMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDR 127
           ++LG +EERRG+L+VM SEREVFELSGPLHLA V+WENA+HRRSVAACLVQGVYILERDR
Sbjct: 18  EQLGSKEERRGILNVMASEREVFELSGPLHLASVDWENANHRRSVAACLVQGVYILERDR 77

Query: 128 QERRQGSEALAPRWWEFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVI 187
           QE+RQG+ A APRWWEFCHFRLLR L+DD DSSIFGAVYEFKP LLQG HK DGSPR+VI
Sbjct: 78  QEKRQGTAAFAPRWWEFCHFRLLRQLVDDVDSSIFGAVYEFKPLLLQGHHKVDGSPRFVI 137

Query: 188 AFRGTLTKPDSVSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLG 247
           AFRGTLTKPDSVSRDIELDLH IQNGLHRTSRFEI MQVVRNMVATVGDSN+WLAGHSLG
Sbjct: 138 AFRGTLTKPDSVSRDIELDLHLIQNGLHRTSRFEIAMQVVRNMVATVGDSNVWLAGHSLG 197

Query: 248 SAMAMLAGKTMARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALK 307
           SAMAMLAG+TMARTGIFLKSY FNPPFF+ PIERIKDKKLKHG+RIAGSVITAGLALALK
Sbjct: 198 SAMAMLAGRTMARTGIFLKSYLFNPPFFAAPIERIKDKKLKHGLRIAGSVITAGLALALK 257

Query: 308 AGS-NSQRNRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLAT 367
           AGS N+QRN  EEPF AI+AWVPSLFVNPSDH+GSEYIGYF+HRK ME++GAGSIERLAT
Sbjct: 258 AGSNNNQRNGIEEPFFAIAAWVPSLFVNPSDHVGSEYIGYFEHRKKMEDIGAGSIERLAT 317

Query: 368 QNSIGGLLMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           QNSIGGLL+NA+GR++EP+HLIPSA LV NLS AQDFKQAHGIHQWWQPH+QL S+I++Y
Sbjct: 318 QNSIGGLLLNALGRESEPIHLIPSAELVINLSPAQDFKQAHGIHQWWQPHLQLHSKIYEY 377

BLAST of Sed0012948 vs. ExPASy TrEMBL
Match: A0A6J1DX98 (GDSL esterase/lipase At4g10955 OS=Momordica charantia OX=3673 GN=LOC111025331 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 3.1e-179
Identity = 302/345 (87.54%), Postives = 328/345 (95.07%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M SEREVFELSGPLHL PV+WENADHRRSVAACLVQGVYILERDRQE+RQGS+ALAPRWW
Sbjct: 1   MASEREVFELSGPLHLTPVDWENADHRRSVAACLVQGVYILERDRQEKRQGSDALAPRWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           EFCHFRL R L+DD DSSIFGA+YEFKP LLQG+HK DGSPRYVIAFRGTLT+PDSVSRD
Sbjct: 61  EFCHFRLFRQLVDDVDSSIFGAIYEFKPVLLQGDHKIDGSPRYVIAFRGTLTRPDSVSRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELDLHF+QNGLHRTSRFEI MQVVRNMVATVGDSN+WLAGHSLGSAMAMLAGKTMARTG
Sbjct: 121 IELDLHFMQNGLHRTSRFEIAMQVVRNMVATVGDSNIWLAGHSLGSAMAMLAGKTMARTG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRSEEPFS 322
           IFL+SY FNPPFF+ PIERIKDKKLKHGIRIAGSVITAGLA ALKA +N+QRNR+EEPF 
Sbjct: 181 IFLESYLFNPPFFAAPIERIKDKKLKHGIRIAGSVITAGLAFALKASNNNQRNRTEEPFF 240

Query: 323 AISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNAIGRDT 382
           AI+AWVPSLFVNPSDHIGSEYIGYF+HRKNMEE+GAGSIERLATQNSIGGLL+NAIGR++
Sbjct: 241 AIAAWVPSLFVNPSDHIGSEYIGYFEHRKNMEEIGAGSIERLATQNSIGGLLLNAIGRES 300

Query: 383 EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKYS 428
           EPLHLIPSA+L  NL+ AQ+FKQAHGIHQWWQPH+QL S+I+KYS
Sbjct: 301 EPLHLIPSASLTVNLAPAQEFKQAHGIHQWWQPHLQLHSKIYKYS 345

BLAST of Sed0012948 vs. TAIR 10
Match: AT4G10955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 7.6e-122
Identity = 210/353 (59.49%), Postives = 275/353 (77.90%), Query Frame = 0

Query: 82  VMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSE-ALAPR 141
           +MISER+ F L+GPLHL  ++W N  HRRSVA  LVQG+Y+ ERDRQ +R+G E AL+P 
Sbjct: 1   MMISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPI 60

Query: 142 WWEFCHFRLLRPLIDDADSSIFGAVYEFK--PRLLQGEHKNDGSPRYVIAFRGTLTKPDS 201
           W EF HFRL+R  +DDAD+SIFG +YE+K   +L Q     + SPR+VIAFRGT+TK DS
Sbjct: 61  WSEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDS 120

Query: 202 VSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTM 261
           +SRDIE D+H I+NGLH T+RFEI +Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+
Sbjct: 121 ISRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTI 180

Query: 262 ARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSN-SQRNRS 321
           ARTG F + + FNPPF S PIE+IKDK++KHGIRIAGSVITAGLALA KA  + SQ +R+
Sbjct: 181 ARTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRA 240

Query: 322 ----EEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGL 381
                +PF A+S W P L+VNP DH+ SEY+GYF+HR  MEE+G G +ER+ATQ+S+GG+
Sbjct: 241 LPAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGM 300

Query: 382 LMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           L+       EP+HLIPS+ L  NLS ++DFKQAHGIHQWW+   + ++++++Y
Sbjct: 301 LLGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Sed0012948 vs. TAIR 10
Match: AT4G10955.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 7.6e-122
Identity = 210/353 (59.49%), Postives = 275/353 (77.90%), Query Frame = 0

Query: 82  VMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSE-ALAPR 141
           +MISER+ F L+GPLHL  ++W N  HRRSVA  LVQG+Y+ ERDRQ +R+G E AL+P 
Sbjct: 1   MMISERDDFSLTGPLHLTSIDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPI 60

Query: 142 WWEFCHFRLLRPLIDDADSSIFGAVYEFK--PRLLQGEHKNDGSPRYVIAFRGTLTKPDS 201
           W EF HFRL+R  +DDAD+SIFG +YE+K   +L Q     + SPR+VIAFRGT+TK DS
Sbjct: 61  WSEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVKSMEFSPRFVIAFRGTVTKVDS 120

Query: 202 VSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTM 261
           +SRDIE D+H I+NGLH T+RFEI +Q VRN+VA+VG S++WLAGHSLG++MA+L GKT+
Sbjct: 121 ISRDIEHDIHVIRNGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGKTI 180

Query: 262 ARTGIFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALALKAGSN-SQRNRS 321
           ARTG F + + FNPPF S PIE+IKDK++KHGIRIAGSVITAGLALA KA  + SQ +R+
Sbjct: 181 ARTGFFPECFAFNPPFLSAPIEKIKDKRIKHGIRIAGSVITAGLALAKKATQHYSQNDRA 240

Query: 322 ----EEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGL 381
                +PF A+S W P L+VNP DH+ SEY+GYF+HR  MEE+G G +ER+ATQ+S+GG+
Sbjct: 241 LPAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGIGFVERVATQHSLGGM 300

Query: 382 LMNAIGRDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           L+       EP+HLIPS+ L  NLS ++DFKQAHGIHQWW+   + ++++++Y
Sbjct: 301 LLGG----QEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETKVYQY 349

BLAST of Sed0012948 vs. TAIR 10
Match: AT2G05260.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 429.9 bits (1104), Expect = 2.4e-120
Identity = 207/359 (57.66%), Postives = 274/359 (76.32%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M S+RE F L GP HL  V+W N DH+R VAACLVQG+YI+E+DRQ +R+G+EALA  WW
Sbjct: 1   MASDREEFNLCGPTHLTTVDWGNEDHQRCVAACLVQGIYIVEQDRQLKREGTEALASPWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           E  +F+L+R L DDAD SIFG ++E+K   LQ +  + G PRYVIAFRGTLTK DS++RD
Sbjct: 61  ESFNFKLIRHLKDDADFSIFGGIFEYKS--LQPDVVDSGVPRYVIAFRGTLTKADSITRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELD+H I+NGLHRTSRFEI MQ VR+M  +VG S+ WL GHSLG+AMA+LAGKTM ++G
Sbjct: 121 IELDIHIIRNGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGKTMGKSG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALA--LKAGSNSQ------R 322
           +++KS  FNPP+ SPPIERI +++++HGIR AGS+ITAGLAL+  LK     Q      +
Sbjct: 181 VYIKSLLFNPPYVSPPIERIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQQQQLQLQ 240

Query: 323 NRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEM--GAGSIERLATQNSIGG 382
           N +E+P  A+S+W+P++ VNP DH+ SEYIG+F+HR NME++  GAG +ER+A Q+S+GG
Sbjct: 241 NLTEDPLEALSSWLPNIHVNPGDHLCSEYIGFFEHRGNMEQIGYGAGIVERMAMQHSLGG 300

Query: 383 LLMNAIGRDT-----EPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQPHMQLQSQIFKY 427
           LLM+A+G        EP+H+IPSANL+ N + ++D+K AHGIHQWW+    L S I+ Y
Sbjct: 301 LLMDAMGVSNAVEVEEPVHVIPSANLIVNKTISEDYKDAHGIHQWWRDDQDLVSHIYMY 357

BLAST of Sed0012948 vs. TAIR 10
Match: AT5G24230.1 (Lipase class 3-related protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.3e-102
Identity = 178/344 (51.74%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 82  VMISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRW 141
           +M S+RE+F +SGP+HL  ++W N+ HR SVA+CLVQ VY LERDRQ+ R G ++ A  W
Sbjct: 14  LMASQREIFSISGPIHLTSIDWNNSYHRTSVASCLVQAVYTLERDRQQNRIGLKSQANHW 73

Query: 142 WEFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGS---PRYVIAFRGTLTKPDS 201
           WEF +F L   LIDD+D SI+GAV+E+K       H    S   PR+VIAFRGT+ KP S
Sbjct: 74  WEFFNFTLAETLIDDSDGSIYGAVFEYKHFFSYNYHHTPHSKPPPRHVIAFRGTILKPHS 133

Query: 202 VSRDIELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTM 261
            SRD++LDL  I++ LH ++RF   +QV+++ VA  G++ +WLAGHSLG+A+A+LAGK M
Sbjct: 134 RSRDLKLDLRCIRDSLHDSTRFVHAIQVIQSAVAKTGNAAVWLAGHSLGAAVALLAGKIM 193

Query: 262 ARTGIFLKSYFFNPPFFSPPIER-IKDKKLKHGIRIAGSVITAGLALALKAGSNSQRNRS 321
            R+G  L+SY FNPPF S PIE+ +K +KLKHG+R AGS++ AG+A+A+K G +  + + 
Sbjct: 194 TRSGFPLESYLFNPPFSSIPIEKLVKSEKLKHGVRFAGSLVKAGVAIAVK-GRHHNKGQE 253

Query: 322 EEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGSIERLATQNSIGGLLMNA 381
           ++ F  +++W+P L++NP D I SEYIGYF+HR  M E+GAG IER+AT+NS+  LL   
Sbjct: 254 DDSFMKLASWIPYLYLNPLDTICSEYIGYFKHRNKMFEIGAGKIERIATRNSLRSLLSGG 313

Query: 382 IG------RDTEPLHLIPSANLVTNLSRAQDFKQAHGIHQWWQP 416
            G        +EPLHL+PSA +  N S++ +FK+AHGIHQWW P
Sbjct: 314 GGGGSSSDSSSEPLHLLPSAYMTINASKSPNFKRAHGIHQWWDP 356

BLAST of Sed0012948 vs. TAIR 10
Match: AT2G05260.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 4.1e-99
Identity = 171/286 (59.79%), Postives = 223/286 (77.97%), Query Frame = 0

Query: 83  MISEREVFELSGPLHLAPVNWENADHRRSVAACLVQGVYILERDRQERRQGSEALAPRWW 142
           M S+RE F L GP HL  V+W N DH+R VAACLVQG+YI+E+DRQ +R+G+EALA  WW
Sbjct: 1   MASDREEFNLCGPTHLTTVDWGNEDHQRCVAACLVQGIYIVEQDRQLKREGTEALASPWW 60

Query: 143 EFCHFRLLRPLIDDADSSIFGAVYEFKPRLLQGEHKNDGSPRYVIAFRGTLTKPDSVSRD 202
           E  +F+L+R L DDAD SIFG ++E+K   LQ +  + G PRYVIAFRGTLTK DS++RD
Sbjct: 61  ESFNFKLIRHLKDDADFSIFGGIFEYKS--LQPDVVDSGVPRYVIAFRGTLTKADSITRD 120

Query: 203 IELDLHFIQNGLHRTSRFEITMQVVRNMVATVGDSNLWLAGHSLGSAMAMLAGKTMARTG 262
           IELD+H I+NGLHRTSRFEI MQ VR+M  +VG S+ WL GHSLG+AMA+LAGKTM ++G
Sbjct: 121 IELDIHIIRNGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGKTMGKSG 180

Query: 263 IFLKSYFFNPPFFSPPIERIKDKKLKHGIRIAGSVITAGLALA--LKAGSNSQ------R 322
           +++KS  FNPP+ SPPIERI +++++HGIR AGS+ITAGLAL+  LK     Q      +
Sbjct: 181 VYIKSLLFNPPYVSPPIERIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQQQQLQLQ 240

Query: 323 NRSEEPFSAISAWVPSLFVNPSDHIGSEYIGYFQHRKNMEEMGAGS 361
           N +E+P  A+S+W+P++ VNP DH+ SEYIG+F+HR NME++G GS
Sbjct: 241 NLTEDPLEALSSWLPNIHVNPGDHLCSEYIGFFEHRGNMEQIGYGS 284