Sed0012427 (gene) Chayote v1

BLAST of Sed0012427 vs. NCBI nr
Match: XP_022991210.1 (TMV resistance protein N-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 801/1128 (71.01%), Postives = 918/1128 (81.38%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRG+DTR  FTDHLY+AF++ GI TF+DD+EMEEGT+ISS
Sbjct: 1    MADSSSSSSSRLWKYDVFLSFRGDDTRGGFTDHLYQAFVQNGIHTFKDDDEMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GM+++PI YKVDPSDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMEVMPIFYKVDPSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRK+LFEIA LKAWV + RS ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKSLFEIAGLKAWVSDNRSLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL--------DDNVILVGIHGMGGIGKTTIAR 240
            KP  +LIEK+++VG+TS LNQL SLLNPN         D+NVILVGIHGMGGIGK+TIAR
Sbjct: 181  KPNFTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDDNDNNVILVGIHGMGGIGKSTIAR 240

Query: 241  VCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISK 300
            VCYE IR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISK
Sbjct: 241  VCYELIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISK 300

Query: 301  AIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEE 360
            AIF KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEE
Sbjct: 301  AIFLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEE 360

Query: 361  LKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNV 420
            L+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKLVGGHPLAL+ LGSSLQNKDL VW+ V
Sbjct: 361  LEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLVGGHPLALQVLGSSLQNKDLSVWDYV 420

Query: 421  IERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA- 480
            IERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A 
Sbjct: 421  IERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAK 480

Query: 481  -NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQ 540
             N+ELLIK SL+TLS  +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I K+V  EASIQ
Sbjct: 481  RNIELLIKNSLITLSPHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KNVVTEASIQ 540

Query: 541  SIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLEL 600
            SIF K+T  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L
Sbjct: 541  SIFLKTTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPL 600

Query: 601  DSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLE 660
            +SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE
Sbjct: 601  NSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLE 660

Query: 661  DCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNG 720
             C NL NIHPSIF AK LTFLS+KDCIN++NLP  ITI  LEV ILSGC KLKK+PEF G
Sbjct: 661  GCTNLSNIHPSIFIAKKLTFLSMKDCINISNLPLQITIHNLEVFILSGCSKLKKLPEFIG 720

Query: 721  NTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGC 780
            NT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL GC
Sbjct: 721  NTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCGC 780

Query: 781  SKLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVP 840
            SKLGN T           ++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +P
Sbjct: 781  SKLGNRTRTLDGSAQRVMIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIP 840

Query: 841  EGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKD 900
            EGIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKD
Sbjct: 841  EGIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKD 900

Query: 901  CISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIPG 960
            CISLKT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIPG
Sbjct: 901  CISLKTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIPG 960

Query: 961  SEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVV 1020
            SEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I ASM++
Sbjct: 961  SEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASMII 1020

Query: 1021 KEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMKV 1080
             E   D  + ++D+ CGH  LVSGMET+DHI MLVLPWT+S         EIEFRF ++V
Sbjct: 1021 -EKELDGSAREEDEACGHVFLVSGMETVDHILMLVLPWTESY-------LEIEFRFGLRV 1080

Query: 1081 NRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            NR HS+ PL + LK+CGVRLINMEE+   V WK N++LLK +F LW K
Sbjct: 1081 NRSHSI-PL-LGLKKCGVRLINMEEDGAAVAWKGNFILLKNSFKLWQK 1116

BLAST of Sed0012427 vs. NCBI nr
Match: XP_023548551.1 (TMV resistance protein N-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 797/1129 (70.59%), Postives = 912/1129 (80.78%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRGEDTR  FTDHLY+AF++ GI TF+DD+EMEEGT+I S
Sbjct: 1    MAASSS-SSSRLWKYDVFLSFRGEDTRGGFTDHLYEAFVQNGIHTFKDDDEMEEGTNIYS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GMK++PI YKVD SDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMKVMPIFYKVDLSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRKALFEIA LKAW  + R+ ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKALFEIAGLKAWASDNRTLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL---------DDNVILVGIHGMGGIGKTTIA 240
            KP L+LIEK+++VG+TS LN L SLLNPN          D+NVILVGIHGMGGIGK+TIA
Sbjct: 181  KPNLTLIEKEKVVGVTSKLNHLFSLLNPNSNDDDDDDDEDNNVILVGIHGMGGIGKSTIA 240

Query: 241  RVCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLIS 300
            RVCYEQIR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LIS
Sbjct: 241  RVCYEQIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALIS 300

Query: 301  KAIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKME 360
            KAI  KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKME
Sbjct: 301  KAISLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKME 360

Query: 361  ELKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNN 420
            EL+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKL GGHPLAL+ LGSSLQNKDL VW+ 
Sbjct: 361  ELEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLAGGHPLALQVLGSSLQNKDLSVWDY 420

Query: 421  VIERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA 480
            VIERL+GGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A
Sbjct: 421  VIERLKGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYA 480

Query: 481  --NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASI 540
              NVELLI+KSL+TLSH  RLQMHDLLQEMGRK+V QKPVRDRLW L +I KSV  EASI
Sbjct: 481  KRNVELLIQKSLITLSHPDRLQMHDLLQEMGRKVVHQKPVRDRLWCLNNI-KSVVTEASI 540

Query: 541  QSIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLE 600
            QSIF KST  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L 
Sbjct: 541  QSIFLKSTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLP 600

Query: 601  LDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDL 660
            L+SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDL
Sbjct: 601  LNSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDL 660

Query: 661  EDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFN 720
            E C NL NIHPSIF AK LTFLS+KDCIN+ NLP  ITI  LEV ILSGC KLKK+PEF 
Sbjct: 661  EGCTNLSNIHPSIFIAKKLTFLSMKDCINITNLPLQITIHNLEVFILSGCSKLKKLPEFI 720

Query: 721  GNTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSG 780
            GNT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL G
Sbjct: 721  GNTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCG 780

Query: 781  CSKLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVV 840
            CSKLGN T          T++WLV  L++AP S VFGI S +G+YSLTEL LS+CNI+ +
Sbjct: 781  CSKLGNRTRTLDGGAQRVTIKWLVLSLYRAPRSGVFGIPSMSGIYSLTELNLSNCNIETI 840

Query: 841  PEGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMK 900
            PEGIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMK
Sbjct: 841  PEGIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMK 900

Query: 901  DCISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIP 960
            DCISLKT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIP
Sbjct: 901  DCISLKTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIP 960

Query: 961  GSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMV 1020
            GSEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I ASM+
Sbjct: 961  GSEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASMI 1020

Query: 1021 VKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMK 1080
            + E   D  + ++D+ CGH  L SGMET+DH+WM VLP T+S         EIEFRF ++
Sbjct: 1021 I-EKELDGSAREEDEACGHVFLGSGMETVDHVWMFVLPCTESY-------LEIEFRFGLR 1080

Query: 1081 VNRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            VN  HS+   +M LK+CGVRLINMEE++  V WK N++LLK +F LW K
Sbjct: 1081 VNPSHSIP--SMGLKKCGVRLINMEEDEAAVAWKGNFILLKNSFRLWQK 1116

BLAST of Sed0012427 vs. NCBI nr
Match: XP_022991209.1 (TMV resistance protein N-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 801/1138 (70.39%), Postives = 918/1138 (80.67%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRG+DTR  FTDHLY+AF++ GI TF+DD+EMEEGT+ISS
Sbjct: 1    MADSSSSSSSRLWKYDVFLSFRGDDTRGGFTDHLYQAFVQNGIHTFKDDDEMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GM+++PI YKVDPSDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMEVMPIFYKVDPSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRK+LFEIA LKAWV + RS ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKSLFEIAGLKAWVSDNRSLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL--------DDNVILVGIHGMGGIGKTTIAR 240
            KP  +LIEK+++VG+TS LNQL SLLNPN         D+NVILVGIHGMGGIGK+TIAR
Sbjct: 181  KPNFTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDDNDNNVILVGIHGMGGIGKSTIAR 240

Query: 241  VCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISK 300
            VCYE IR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISK
Sbjct: 241  VCYELIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISK 300

Query: 301  AIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEE 360
            AIF KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEE
Sbjct: 301  AIFLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEE 360

Query: 361  LKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNV 420
            L+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKLVGGHPLAL+ LGSSLQNKDL VW+ V
Sbjct: 361  LEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLVGGHPLALQVLGSSLQNKDLSVWDYV 420

Query: 421  IERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA- 480
            IERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A 
Sbjct: 421  IERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAK 480

Query: 481  -NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQ 540
             N+ELLIK SL+TLS  +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I K+V  EASIQ
Sbjct: 481  RNIELLIKNSLITLSPHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KNVVTEASIQ 540

Query: 541  SIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLEL 600
            SIF K+T  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L
Sbjct: 541  SIFLKTTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPL 600

Query: 601  DSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLE 660
            +SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE
Sbjct: 601  NSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLE 660

Query: 661  DCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNG 720
             C NL NIHPSIF AK LTFLS+KDCIN++NLP  ITI  LEV ILSGC KLKK+PEF G
Sbjct: 661  GCTNLSNIHPSIFIAKKLTFLSMKDCINISNLPLQITIHNLEVFILSGCSKLKKLPEFIG 720

Query: 721  NTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGC 780
            NT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL GC
Sbjct: 721  NTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCGC 780

Query: 781  SKLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVP 840
            SKLGN T           ++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +P
Sbjct: 781  SKLGNRTRTLDGSAQRVMIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIP 840

Query: 841  EGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKD 900
            EGIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKD
Sbjct: 841  EGIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKD 900

Query: 901  CISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSW----------MQKMFFH 960
            CISLKT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSW          MQK  F 
Sbjct: 901  CISLKTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQWMMSGFCDMQKTAFQ 960

Query: 961  KEKFSIMIPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIP 1020
             EK SIMIPGSEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ 
Sbjct: 961  TEKLSIMIPGSEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVV 1020

Query: 1021 SLGIFASMVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCT 1080
            S  I ASM++ E   D  + ++D+ CGH  LVSGMET+DHI MLVLPWT+S         
Sbjct: 1021 SFAISASMII-EKELDGSAREEDEACGHVFLVSGMETVDHILMLVLPWTESY-------L 1080

Query: 1081 EIEFRFHMKVNRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            EIEFRF ++VNR HS+ PL + LK+CGVRLINMEE+   V WK N++LLK +F LW K
Sbjct: 1081 EIEFRFGLRVNRSHSI-PL-LGLKKCGVRLINMEEDGAAVAWKGNFILLKNSFKLWQK 1126

BLAST of Sed0012427 vs. NCBI nr
Match: KAG6575987.1 (TMV resistance protein N, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1521.9 bits (3939), Expect = 0.0e+00
Identity = 794/1124 (70.64%), Postives = 912/1124 (81.14%), Query Frame = 0

Query: 4    SSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELL 63
            S S SS+RLWKYDVFLSFRGEDTR  FT HLY+AF+R GI TF+DD+EMEEGT+ISSEL 
Sbjct: 8    SLSSSSSRLWKYDVFLSFRGEDTRGGFTGHLYEAFVRNGIHTFKDDDEMEEGTNISSELF 67

Query: 64   AAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSF 123
            AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GMK++PI YKVD SDVRNQR SF
Sbjct: 68   AAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMKVMPIFYKVDLSDVRNQRGSF 127

Query: 124  KKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRIKPT 183
             KAF+KHE RFG++   VL WRKALFEIA LKAWV + R+ ES+  EDICEAILRRIKP 
Sbjct: 128  GKAFLKHEARFGEDDSKVLQWRKALFEIAGLKAWVSDNRTLESKLKEDICEAILRRIKPN 187

Query: 184  LSLIEKDQLVGITSNLNQLSSLLNPNL-------DDNVILVGIHGMGGIGKTTIARVCYE 243
            L+LIEK+++VG+TS LNQL SLLNPN        D+NVILVGIHGMGGIGK+TIARVCYE
Sbjct: 188  LTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDEDNNVILVGIHGMGGIGKSTIARVCYE 247

Query: 244  QIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISKAIFQ 303
            QIR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G VLISKAI  
Sbjct: 248  QIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRVLISKAISL 307

Query: 304  KKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYK 363
            KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEEL+Y+
Sbjct: 308  KKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEELEYE 367

Query: 364  EALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERL 423
            E+LQLLSLNAFKERCP+EGYLG S+ IVKL GGHPLAL+ LGSSLQNKDL VW+ VIERL
Sbjct: 368  ESLQLLSLNAFKERCPREGYLGQSDRIVKLAGGHPLALQVLGSSLQNKDLSVWDYVIERL 427

Query: 424  QGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA--NVE 483
            QGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A  N+E
Sbjct: 428  QGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAKRNIE 487

Query: 484  LLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQSIFF 543
            LLI+KSL+TLSH +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I KSV  EASIQSIF 
Sbjct: 488  LLIQKSLVTLSHHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KSVVTEASIQSIFL 547

Query: 544  KSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELDSEL 603
            KST  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L+SE+
Sbjct: 548  KSTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPLNSEV 607

Query: 604  EYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLEDCPN 663
            +Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE C N
Sbjct: 608  DYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLEGCTN 667

Query: 664  LINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNGNTQR 723
            L NIHPSIF AK LTFLS+K+CIN+ NLP  ITI  LEV ILSGC KLKK+PEF GNT  
Sbjct: 668  LSNIHPSIFIAKKLTFLSMKNCINITNLPLQITIHNLEVFILSGCSKLKKLPEFIGNTDS 727

Query: 724  LQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCSKLG 783
            L +L LDGT I ELPSSVASL+ L VL+L NCK LVS++ + N L SL+SLNL GCSKLG
Sbjct: 728  LLELRLDGTYIAELPSSVASLNRLTVLNLMNCKKLVSVSFSFNRLSSLESLNLCGCSKLG 787

Query: 784  NPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVPEGIE 843
            N T          T++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +PEGIE
Sbjct: 788  NRTRTSDSGAQRATIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIPEGIE 847

Query: 844  CLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISL 903
             LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKDCISL
Sbjct: 848  GLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKDCISL 907

Query: 904  KTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIPGSEIP 963
            KT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIPGSEIP
Sbjct: 908  KTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIPGSEIP 967

Query: 964  SWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVVKEPN 1023
             WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I AS+++ E  
Sbjct: 968  DWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASIII-EKE 1027

Query: 1024 HDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMKVNRYH 1083
             D  + ++D+ CGH  L SGMET+DH+WM VLP T+S         EIEFRF ++VN   
Sbjct: 1028 LDGSAREEDEACGHVFLGSGMETVDHVWMFVLPCTESY-------LEIEFRFGLRVNPSR 1087

Query: 1084 SVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            S+   +M+LK+CGVRLINMEE++  V WK N++LLK +F LW K
Sbjct: 1088 SIP--SMDLKKCGVRLINMEEDEAAVAWKGNFILLKNSFRLWQK 1119

BLAST of Sed0012427 vs. NCBI nr
Match: XP_022953344.1 (TMV resistance protein N-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 794/1127 (70.45%), Postives = 911/1127 (80.83%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS  S+RLWKYDVFLSFRGEDTR  FT HLY+AF+R GI TF+DD++MEEGT+ISS
Sbjct: 1    MAASSSSLSSRLWKYDVFLSFRGEDTRGGFTGHLYEAFVRNGIHTFKDDDDMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GMK++PI YKVD SDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMKVMPIFYKVDLSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRKALFEIA LKAWV + R+ ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKALFEIAGLKAWVSDNRTLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL-------DDNVILVGIHGMGGIGKTTIARV 240
            KP L+LIEK+++VG+TS LNQL SLLNPN        D+NVILVGIHGMGGIGK+TIARV
Sbjct: 181  KPNLTLIEKEKVVGVTSKLNQLFSLLNPNTNDDDDDEDNNVILVGIHGMGGIGKSTIARV 240

Query: 241  CYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISKA 300
            CYEQIR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISKA
Sbjct: 241  CYEQIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISKA 300

Query: 301  IFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEEL 360
            I  KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEEL
Sbjct: 301  ISLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEEL 360

Query: 361  KYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVI 420
            +Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKL GGHPLAL+ LGSSLQNKDL VW+ VI
Sbjct: 361  EYEESLQLLSLNAFKERCPREGYLGQSDRIVKLAGGHPLALQVLGSSLQNKDLSVWDYVI 420

Query: 421  ERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA-- 480
            ERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A  
Sbjct: 421  ERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAKR 480

Query: 481  NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQS 540
            N+ELLI+KSL+TLSH +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I KSV  EASIQS
Sbjct: 481  NIELLIQKSLVTLSHHNRLQMHDLLQEMGRKVVPQKPVRDRLWCLKNI-KSVVTEASIQS 540

Query: 541  IFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELD 600
            IF KST  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L+
Sbjct: 541  IFLKSTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPLN 600

Query: 601  SELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLED 660
            SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE 
Sbjct: 601  SEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLEG 660

Query: 661  CPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNGN 720
            C NL NIHPSIF AK LTFLS+K+CIN+ NLP  ITI  LEV ILSGC KLKK+PEF GN
Sbjct: 661  CTNLSNIHPSIFIAKKLTFLSMKNCINITNLPLQITIHNLEVFILSGCSKLKKLPEFIGN 720

Query: 721  TQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCS 780
            T  L +L LDGT I ELPSSVASL+ L VL+L NCK LVS++ + N + SL+SLNL GCS
Sbjct: 721  TDSLLELRLDGTYIAELPSSVASLNRLTVLNLMNCKKLVSVSFSFNRISSLESLNLCGCS 780

Query: 781  KLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVPE 840
            KLGN T          T++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +PE
Sbjct: 781  KLGNRTRTSDSGAQHATIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIPE 840

Query: 841  GIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDC 900
            GIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKDC
Sbjct: 841  GIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKDC 900

Query: 901  ISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIPGS 960
            ISLKT   ISKMD LY+M  +NL NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIPGS
Sbjct: 901  ISLKTSTAISKMDRLYFMTGVNLQNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIPGS 960

Query: 961  EIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVVK 1020
            EIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I ASM++ 
Sbjct: 961  EIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASMII- 1020

Query: 1021 EPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMKVN 1080
            E   D  + ++D+ CGH  L SGMET+DH+WM VLP T+S         EIEFRF ++VN
Sbjct: 1021 EKELDGSAREEDEACGHVFLSSGMETVDHVWMFVLPCTESY-------LEIEFRFGLRVN 1080

Query: 1081 RYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
              HS+   +M LK+CGVRLINMEE++  V WK N++LLK +F LW K
Sbjct: 1081 PSHSIP--SMGLKKCGVRLINMEEDEAAVAWKGNFILLKNSFRLWQK 1115

BLAST of Sed0012427 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 9.3e-148
Identity = 367/946 (38.79%), Postives = 515/946 (54.44%), Query Frame = 0

Query: 4   SSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELL 63
           SS+ S  R   YDVFLSFRGEDTR NFTDHLY A  R+GI TFRDD  +  G  I+ ELL
Sbjct: 13  SSTPSIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRDD-RLRRGEAIAPELL 72

Query: 64  AAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSF 123
            AIE SR++V+V S++YA SRWCL ELVKI EC++  G  + PI Y VDPS VR Q  SF
Sbjct: 73  KAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGSF 132

Query: 124 KKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRIKPT 183
            +AF  +E+ +      +  WR AL E A+L  W L +  +ES  I++I  +I R++K  
Sbjct: 133 GEAFAGYEENWKD---KIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLK-C 192

Query: 184 LSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREEFE 243
             L     LVGI S++ ++   L+    D V +VGI+G+GGIGKTTIA+V Y ++  EFE
Sbjct: 193 KRLDVGANLVGIDSHVKEMILRLHLESSD-VRMVGIYGVGGIGKTTIAKVIYNELSCEFE 252

Query: 244 VHCFLSNVREQVETSKITHIQSKLLSRI---TFSKNIDIWDEEEGMVLISKAIFQKKTLL 303
              FL N+RE      + H+Q++LL  I     S+NI        M  I   +  ++  +
Sbjct: 253 YMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASM--IKDILLSRRVFI 312

Query: 304 VLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQL 363
           VLDDVD   Q+  L    E + G GSR+IITTRN+ +L+   V  ++++E L ++EA +L
Sbjct: 313 VLDDVDDLSQLEYLLGHRE-WLGEGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEEACEL 372

Query: 364 LSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWS 423
            SL AFK+  PK  Y   +  +V    G PLALK LGS L  K +  W   +++L     
Sbjct: 373 FSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPK 432

Query: 424 IDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA--NVELLIKK 483
            D  I K LK SYDGLD  +K IFLD+ACFFKG+ RD V  IL GCDF A   +  L   
Sbjct: 433 AD--IHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDL 492

Query: 484 SLLTLSHDSRLQMHDLLQEMGRKIVCQK-PVR----DRLWGLKDINKSVGMEASIQSIFF 543
            L+TL + +++ MHDL+Q+MG +IV +  PV      RLW   D  +++  +  I+S+  
Sbjct: 493 CLITLPY-NQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVET 552

Query: 544 KS-----TRNVVPIPIMFSRMHQLRLINFRN--------------------------VKL 603
            S      + V     +F++M +LRL+   +                           K+
Sbjct: 553 MSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKM 612

Query: 604 QNQSEYCIPS-ELRYLKWKRYPMEVLELDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQ 663
           Q    +  PS ELRYL+W  YP++ L L+ +   KL+ELH+  SN+KQ W G K LE+L+
Sbjct: 613 QLGQSFKFPSYELRYLRWDGYPLDSLPLNFD-GGKLVELHLKCSNIKQLWQGHKDLERLK 672

Query: 664 YIKVSHSQNLYQTPNFTQLPNLQILDLEDCPNLINIHPSIFTAKSLTFLSLKDCINLNNL 723
            I +S+S+ L Q   F+ +PNL+ L L  C +LI+IHPS+   K LT LSL+ C  L NL
Sbjct: 673 VIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNL 732

Query: 724 PGHI-TIEILEVLILSGCLKLKKIPEFNGNTQRLQQLYLDGTSITELPSSVASLDCLRVL 783
           P  I  +E LE L LS C K +K PE  GN + L +L L  T+I +LP S+  L+ L  L
Sbjct: 733 PDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLESL 792

Query: 784 SLKNCKMLVSITSATNWLPSLKSLNLSGCSKLGNPTSETTLEWLVSRLFKAPTSRVFGIA 843
            L NC            + SL  L+L   +    P S   LE                  
Sbjct: 793 YLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLE------------------ 852

Query: 844 SFAGLYSLTELILSDC-NIDVVPEGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNI 902
                 SL  L LSDC   +  PE    +  L+ L+L N +  +LP  I  L +L+ L++
Sbjct: 853 ------SLEILNLSDCAKFEKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSL 912

BLAST of Sed0012427 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.7e-146
Identity = 370/944 (39.19%), Postives = 518/944 (54.87%), Query Frame = 0

Query: 4   SSSLSST----RLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDIS 63
           SSS SST    R   YDVFLSFRGEDTR NFTDHLY A  R+GI TFRDD ++  G  I+
Sbjct: 12  SSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRDD-KLRRGEAIA 71

Query: 64  SELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFEC---KEQKGMKIIPIIYKVDPSDV 123
            ELL AIE SR++V+V S++YA SRWCL ELVKI EC   K+  G  + PI Y VDPS V
Sbjct: 72  PELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHV 131

Query: 124 RNQRQSFKKAFVKHEKRFGKNSLN-VLSWRKALFEIASLKAWVLNERSHESEFIEDICEA 183
           R Q  SF +AF      +G+N  + +  WR AL E A+L  W L +  +ES  I++I ++
Sbjct: 132 RKQEGSFGEAFA----GYGENLKDKIPRWRTALTEAANLSGWPLQD-GYESNQIKEITDS 191

Query: 184 ILRRIKPTLSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCY 243
           I RR+K    L     LVGI S++ ++   L+    D V +VG++G+GGIGKTTIA+V Y
Sbjct: 192 IFRRLK-CKRLDAGANLVGIDSHVKEMIWRLHMESSD-VRMVGMYGVGGIGKTTIAKVIY 251

Query: 244 EQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRI---TFSKNIDIWDEEEGMVLISKA 303
            ++  EFE   FL N+RE+  T  ++ +Q++LL  I     S+NI+      G  +I   
Sbjct: 252 NELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNIN--SVAHGASMIKDI 311

Query: 304 IFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEEL 363
           +  K   +VLDDVD   Q+  L    E + G GSR+IITTRN+ +L    V  +++++ L
Sbjct: 312 LSSKIVFIVLDDVDDQSQLEYLLRHRE-WLGEGSRVIITTRNKHVLDVQKVDDLYEVKGL 371

Query: 364 KYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVI 423
            ++EA +L SL AF++  PK  Y   S+ +V    G PLALK LG  L  K +  W + +
Sbjct: 372 NFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESEL 431

Query: 424 ERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHANV 483
            +L      + +I   LK SYDGL   EK IFLDVACFFKG+ RD V +IL  CDFHA +
Sbjct: 432 RKLDR--EPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEI 491

Query: 484 EL--LIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQK----PVR-DRLWGLKDINKSVGME 543
            +  L  K L+TL + +R++MHDL+Q+MG +IV +K    P +  RLW   D  +++   
Sbjct: 492 GIKNLNDKCLITLQY-NRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAY 551

Query: 544 ASIQ-----SIFFKSTRNVVPIPIMFSRMHQLRLINFR---------------------N 603
             I+     S+     + V      F++M +LRL+  +                     +
Sbjct: 552 KGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYD 611

Query: 604 VKLQNQSE------YCIPS-ELRYLKWKRYPMEVLELDSELEYKLIELHMCSSNLKQFWH 663
           V ++N S+      +  PS ELRYL+W  YP++ L  + +   KL+ELH+  SN+KQ   
Sbjct: 612 VVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFD-GGKLVELHLKCSNIKQLRL 671

Query: 664 GEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLEDCPNLINIHPSIFTAKSLTFLSL 723
           G K LE L+ I +S+S+ L Q   F+ +PNL+ L L  C +LI+IHPS+   K LT LSL
Sbjct: 672 GNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSL 731

Query: 724 KDCINLNNLPGHI-TIEILEVLILSGCLKLKKIPEFNGNTQRLQQLYLDGTSITELPSSV 783
           K C  L NLP  I  +E LE+L L+ C K +K PE  GN + L +L L  T+I +LP S+
Sbjct: 732 KSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPDSI 791

Query: 784 ASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCSKLGNPTSETTLEWLVSRLFKA 843
             L+ L+ L L +C            + SL+ L+L   +    P S   LE L  RL+ +
Sbjct: 792 GDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESL-ERLYLS 851

Query: 844 PTSRVFGIASFAG-LYSLTELILSDCNIDVVPEGIECLVWLRVLNLSNNSFCE------- 871
             S+        G + SL EL L +  I  +P+ I  L  L+ L+LSN S  E       
Sbjct: 852 YCSKFEKFPEKGGNMKSLMELDLQNTAIKDLPDSIGDLESLKYLDLSNCSKFEKFPEKGG 911

BLAST of Sed0012427 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.2e-140
Identity = 331/882 (37.53%), Postives = 486/882 (55.10%), Query Frame = 0

Query: 15  YDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELLAAIEVSRAAVV 74
           YDVFLSFRGEDTR  F  HL+ A I KGI TF DD E++ G  ISSEL+ AI  SR AVV
Sbjct: 12  YDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVV 71

Query: 75  VVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSFKKAFVKHEKRF 134
           V SK+YASS WCL ELVKI E  E+  + ++P+ Y VDPS VR Q   +   F K E   
Sbjct: 72  VFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANL 131

Query: 135 GKNSLNVLSWRKALFEIASLKAWVLNE--RSHESEFIEDICEAILRRIKPTLSLIEKDQL 194
             +   VL WR+AL ++A++    L       ES+ I+ I + I  +   ++S+  +D L
Sbjct: 132 VDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRD-L 191

Query: 195 VGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREEFEVHCFLSNVR 254
           VGI S + +LSSLL  +L   V LVGI GMGG+GKTT AR  + +  + FE  CFL +V+
Sbjct: 192 VGIESQIKKLSSLLRMDL-KGVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVK 251

Query: 255 EQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLVLDDVDHFDQMT 314
           E ++   + ++Q  LLS++   + +D  D EE  V++ + +  KK L+VLDDV+H DQ+ 
Sbjct: 252 EYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLD 311

Query: 315 KLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLLSLNAFKERCPK 374
           KL    E +FG GSRI+ITTR+  LL    V   ++++ L+  EA++L +L+AFK   P+
Sbjct: 312 KLVGA-EDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPE 371

Query: 375 EGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWSIDEKIFKCLKVS 434
           + +    N +V   GG PLALK LGS L  +DL VW + I+RL+   + + +I   LK+S
Sbjct: 372 KEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKD--NPEGEIMATLKIS 431

Query: 435 YDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFH--ANVELLIKKSLLTLSHDSRLQ 494
           +DGL D EK IFLD+ACFF+G  +  +  +     FH    V+ L++KSL+ +  D ++Q
Sbjct: 432 FDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILED-KIQ 491

Query: 495 MHDLLQEMGRKIVCQKPVRDRLWGLKDINKS-VG--MEASIQSIFFKSTRNVV--PIPIM 554
           MHDL+QEMGR+I  Q+    R++  +D+  + +G   + +I+ +            +  M
Sbjct: 492 MHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEYM 551

Query: 555 FS-----RMHQLRLI--NFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELDSELEYKLI 614
           +S     +  +LR++   + N        Y +P+ L +L+W+ Y       + E   KL+
Sbjct: 552 YSAEALKKTRRLRILVKEYYNRGFDEPVAY-LPNSLLWLEWRNYSSNSFPSNFE-PSKLV 611

Query: 615 ELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLEDCPNLINIH 674
            L M  S++ + W+G K L  L  + +S+   L QTP+F  + NL+ L L  C  L+ +H
Sbjct: 612 YLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVH 671

Query: 675 PSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNGNTQRLQQLY 734
           PS+   K+L  L++  CI+L  LP  I  E LEVL L+ C  LK  PE   N   L++L 
Sbjct: 672 PSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVERNMTHLKKLD 731

Query: 735 LDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCSKLGNPTSE 794
           L  T I ELP+S+  L  L  L + +C  LVS+ S+  W    ++L +S C KLG+    
Sbjct: 732 LTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSI-W--RFRNLKISECEKLGS---- 791

Query: 795 TTLEWLVSRLFKAPTSRVFGIASFAGLYSLT-ELILSDCNIDVVPEGIECLVWLRVLNLS 854
                               +    G  + T ELIL   +I  +P  I  L  L  L + 
Sbjct: 792 --------------------LPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEIC 851

Query: 855 N-NSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKL 879
           N  +   L   I  L +L  L +  C+KL  +P +P  I  L
Sbjct: 852 NCKTISSLSSSIWGLTSLTTLKLLDCRKLKNLPGIPNAINHL 858

BLAST of Sed0012427 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.4e-140
Identity = 359/1014 (35.40%), Postives = 550/1014 (54.24%), Query Frame = 0

Query: 5    SSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELLA 64
            +S SS+  W YDVFLSFRGEDTR  FT HLY+    KGI TF+DD  +E G  I  EL  
Sbjct: 2    ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61

Query: 65   AIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSFK 124
            AIE S+ A+VV S++YA+SRWCL ELVKI ECK +    +IPI Y VDPS VRNQ++SF 
Sbjct: 62   AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121

Query: 125  KAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI-KPT 184
            KAF +HE ++  +   +  WR AL E A+LK    N    +++ I  I + I  ++ K +
Sbjct: 122  KAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKIS 181

Query: 185  LSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQI----- 244
            LS ++   +VGI ++L ++ SLL   + + V ++GI GMGG+GKTTIAR  ++ +     
Sbjct: 182  LSYLQ--NIVGIDTHLEKIESLLEIGI-NGVRIMGIWGMGGVGKTTIARAIFDTLLGRMD 241

Query: 245  -REEFEVHCFLSNVREQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKK 304
               +F+  CFL +++E      +  +Q+ LLS +   K  +  +EE+G   ++  +  KK
Sbjct: 242  SSYQFDGACFLKDIKE--NKRGMHSLQNALLSELLREK-ANYNNEEDGKHQMASRLRSKK 301

Query: 305  TLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEA 364
             L+VLDD+D+ D   +    +  +FG GSRIIITTR++ L+    +  ++++  L   E+
Sbjct: 302  VLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDI--IYEVTALPDHES 361

Query: 365  LQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQG 424
            +QL   +AF +  P E +   S  +V    G PLALK  GS L N  L  W + IE ++ 
Sbjct: 362  IQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKN 421

Query: 425  GWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA--NVELL 484
              +    I   LK+SYDGL+ +++E+FLD+ACF +G+++D + +IL  C   A   + +L
Sbjct: 422  --NSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRIL 481

Query: 485  IKKSLLTLSHDSRLQMHDLLQEMGRKIV-CQKP--VRDRLWGLKDINKSVGMEA---SIQ 544
            I KSL+ +S  +++QMHDL+Q+MG+ IV  QK    R RLW  K++ + +       +++
Sbjct: 482  IDKSLVFISEYNQVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGTMAME 541

Query: 545  SIFFKSTRNVVPIPIM-FSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLE 604
            +I+  S  + +         M +LR+ N          +Y +P+ LR      YP E   
Sbjct: 542  AIWVSSYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDY-LPNNLRCFVCTNYPWESFP 601

Query: 605  LDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDL 664
               EL+  L+ L +  ++L+  W   K+L  L+ I +S S+ L +TP+FT +PNL+ ++L
Sbjct: 602  STFELK-MLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNL 661

Query: 665  EDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFN 724
              C NL  +H S+     +  L L DC +L   P  + +E LE L L  C  L+K+PE  
Sbjct: 662  YQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFP-CVNVESLEYLGLRSCDSLEKLPEIY 721

Query: 725  GNTQRLQQLYLDGTSITELPSSVASLDC-LRVLSLKNCKMLVSITSATNWLPSLKSLNLS 784
            G  +   Q+++ G+ I ELPSS+      +  L L N K LV++ S+   L SL SL++S
Sbjct: 722  GRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVS 781

Query: 785  GCSKLGN------------------------PTSETTLEWLVSRLFKAPTSRV-FGIASF 844
            GCSKL +                        P+S   L  L+  +F+     V F     
Sbjct: 782  GCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFRGFKDGVHFEFPPV 841

Query: 845  A-GLYSLTELILSDCNI--DVVPEGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNI 904
            A GL+SL  L LS CN+    +PE I  L  L+ L+LS N+F  LP  IA+L  L+ L++
Sbjct: 842  AEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSSIAQLGALQSLDL 901

Query: 905  NGCKKLVQVPELPERIMKLKMKDCISLKTFPDI---SKMDHLYYMGEMNLINCYQLADNK 964
              C++L Q+PELP  + +L +   ++LK    +    K  H   + + +    Y L    
Sbjct: 902  KDCQRLTQLPELPPELNELHVDCHMALKFIHYLVTKRKKLHRVKLDDAHNDTMYNLFAYT 961

Query: 965  QIQSLLTSWMQKMFFHKEKFSIMI----PGSE-IPSWFTTTKTGSSISIKWDPN 966
              Q++  S M+      +  S+ +    P  E IPSWF      SS+S+    N
Sbjct: 962  MFQNI--SSMRHDISASDSLSLTVFTGQPYPEKIPSWFHHQGWDSSVSVNLPEN 1000

BLAST of Sed0012427 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.8e-136
Identity = 385/1192 (32.30%), Postives = 598/1192 (50.17%), Query Frame = 0

Query: 4    SSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELL 63
            SSS SS    ++DVFLSFRG DTR NFT HL KA   +GI +F DD  +  G ++++ L 
Sbjct: 3    SSSPSSA---EFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLTA-LF 62

Query: 64   AAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSF 123
              IE S+ A++V S +YA+S WCL ELVKI EC+      ++PI YKVD SDV  QR SF
Sbjct: 63   DRIEKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSF 122

Query: 124  KKAFVKHEKRF-GKNSLNVLSWRKALFEIASLKAWVLNERS-HESEFIEDICEAILRRIK 183
               F   E  F G     + SW+ AL   +++  +V+ E S  E++ +++I     +++ 
Sbjct: 123  AVPFKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN 182

Query: 184  PTLSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREE 243
              L+    + LVGI S L  L  LL+    D V ++GI GM GIGKTT+A   Y ++R +
Sbjct: 183  -DLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQ 242

Query: 244  FEVHCFLSNVREQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLV 303
            F+  CFL+N+RE    S +  +  KL S +   ++++I           + +  K+ L+V
Sbjct: 243  FDGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIV 302

Query: 304  LDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLL 363
            LDDV+   Q+  L    + Y G GSRIIITTR+  L+ T+   R + + +L  +EAL+L 
Sbjct: 303  LDDVNDEKQIRYLMGHCKWYQG-GSRIIITTRDSKLIETI-KGRKYVLPKLNDREALKLF 362

Query: 364  SLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWSI 423
            SLNAF    P + + G +N ++    GHPLALK LGS L  +D   W   ++RL+     
Sbjct: 363  SLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHG 422

Query: 424  DEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEIL--VGCDFHANVELLIKKS 483
            D  I++ L+ SY+ L  E+K +FLD+ACFF+ +  D V  +L   G D    V+ L+ K 
Sbjct: 423  D--IYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKC 482

Query: 484  LLTLSHDSRLQMHDLLQEMGRKIVCQKP---VRD---------------RLWGLKDI--- 543
            L+TLS D+R++MHD+LQ M ++I  +     +RD               RLW  +DI   
Sbjct: 483  LITLS-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDL 542

Query: 544  -NKSVGMEASIQSIFFKST--RNVVPIPIMFSRMHQLRLINFRNVKLQNQSE-------- 603
              + +G +  I+ IF  ++  R +      F  M+ L+ +   +       E        
Sbjct: 543  LTEGLGTD-KIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLR 602

Query: 604  ---YCIPSELRYLKWKRYPMEVLELDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIK 663
                 +P+EL YL W  YP++ + LD + +  L++L +  S L++ W  EK +  L+++ 
Sbjct: 603  RGLSFLPNELTYLHWHGYPLQSIPLDFDPK-NLVDLKLPHSQLEEIWDDEKDVGMLKWVD 662

Query: 664  VSHSQNLYQTPNFTQLPNLQILDLEDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGH 723
            +SHS NL Q        NL+ L+LE C +L  +  +I   + L +L+L+DC +L +LP  
Sbjct: 663  LSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKG 722

Query: 724  ITIEILEVLILSGCLKLKKIPEFNGNTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKN 783
            I  + L+ LILSGC  LKK P  + N   ++ L LDGT I  LP S+ +   L +L+LKN
Sbjct: 723  IKTQSLQTLILSGCSSLKKFPLISEN---VEVLLLDGTVIKSLPESIQTFRRLALLNLKN 782

Query: 784  CKMLVSITSATNWLPSLKSLNLSGCSKLG-----------------NPTSETTLEWLVSR 843
            CK L  ++S    L  L+ L LSGCS+L                  + TS T +  ++  
Sbjct: 783  CKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMM-H 842

Query: 844  LFKAPTSRVFGIASFA-----------GLYSLTELILSDCNIDVVPEGIECLVWLRVLNL 903
            L    T  + G +S             G   LT+L LS C++  +P+ I  L  L+ L L
Sbjct: 843  LSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCL 902

Query: 904  SNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLKTFPD----- 963
            S N+   LP    +L+NL+  ++  CK L  +P LP+ +  L   +C SL+T  +     
Sbjct: 903  SGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPL 962

Query: 964  -ISKMDHLYYMGEMNLINCYQLADNKQI---------QSLLTSWMQKMFFH----KEKFS 1023
             + +  H  ++      NCY+L  + Q            L+ +   K ++     +    
Sbjct: 963  TVGERIHSMFI----FSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVG 1022

Query: 1024 IMIPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNG---TIPSL 1083
            I  P +EIPSWF   + G S+ I   P+  + N +  AL VV    D + +    ++   
Sbjct: 1023 ICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCC 1082

Query: 1084 GIFASMVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLP--WTKSMWDEINKC- 1101
            G F +        D      ++ CG     S   T DH++M        K++  E N C 
Sbjct: 1083 GNFENKDSSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCC 1142

BLAST of Sed0012427 vs. ExPASy TrEMBL
Match: A0A6J1JS91 (TMV resistance protein N-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487933 PE=4 SV=1)

HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 801/1128 (71.01%), Postives = 918/1128 (81.38%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRG+DTR  FTDHLY+AF++ GI TF+DD+EMEEGT+ISS
Sbjct: 1    MADSSSSSSSRLWKYDVFLSFRGDDTRGGFTDHLYQAFVQNGIHTFKDDDEMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GM+++PI YKVDPSDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMEVMPIFYKVDPSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRK+LFEIA LKAWV + RS ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKSLFEIAGLKAWVSDNRSLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL--------DDNVILVGIHGMGGIGKTTIAR 240
            KP  +LIEK+++VG+TS LNQL SLLNPN         D+NVILVGIHGMGGIGK+TIAR
Sbjct: 181  KPNFTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDDNDNNVILVGIHGMGGIGKSTIAR 240

Query: 241  VCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISK 300
            VCYE IR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISK
Sbjct: 241  VCYELIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISK 300

Query: 301  AIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEE 360
            AIF KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEE
Sbjct: 301  AIFLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEE 360

Query: 361  LKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNV 420
            L+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKLVGGHPLAL+ LGSSLQNKDL VW+ V
Sbjct: 361  LEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLVGGHPLALQVLGSSLQNKDLSVWDYV 420

Query: 421  IERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA- 480
            IERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A 
Sbjct: 421  IERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAK 480

Query: 481  -NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQ 540
             N+ELLIK SL+TLS  +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I K+V  EASIQ
Sbjct: 481  RNIELLIKNSLITLSPHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KNVVTEASIQ 540

Query: 541  SIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLEL 600
            SIF K+T  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L
Sbjct: 541  SIFLKTTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPL 600

Query: 601  DSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLE 660
            +SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE
Sbjct: 601  NSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLE 660

Query: 661  DCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNG 720
             C NL NIHPSIF AK LTFLS+KDCIN++NLP  ITI  LEV ILSGC KLKK+PEF G
Sbjct: 661  GCTNLSNIHPSIFIAKKLTFLSMKDCINISNLPLQITIHNLEVFILSGCSKLKKLPEFIG 720

Query: 721  NTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGC 780
            NT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL GC
Sbjct: 721  NTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCGC 780

Query: 781  SKLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVP 840
            SKLGN T           ++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +P
Sbjct: 781  SKLGNRTRTLDGSAQRVMIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIP 840

Query: 841  EGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKD 900
            EGIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKD
Sbjct: 841  EGIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKD 900

Query: 901  CISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIPG 960
            CISLKT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIPG
Sbjct: 901  CISLKTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIPG 960

Query: 961  SEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVV 1020
            SEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I ASM++
Sbjct: 961  SEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASMII 1020

Query: 1021 KEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMKV 1080
             E   D  + ++D+ CGH  LVSGMET+DHI MLVLPWT+S         EIEFRF ++V
Sbjct: 1021 -EKELDGSAREEDEACGHVFLVSGMETVDHILMLVLPWTESY-------LEIEFRFGLRV 1080

Query: 1081 NRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            NR HS+ PL + LK+CGVRLINMEE+   V WK N++LLK +F LW K
Sbjct: 1081 NRSHSI-PL-LGLKKCGVRLINMEEDGAAVAWKGNFILLKNSFKLWQK 1116

BLAST of Sed0012427 vs. ExPASy TrEMBL
Match: A0A6J1JU68 (TMV resistance protein N-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487933 PE=4 SV=1)

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 801/1138 (70.39%), Postives = 918/1138 (80.67%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRG+DTR  FTDHLY+AF++ GI TF+DD+EMEEGT+ISS
Sbjct: 1    MADSSSSSSSRLWKYDVFLSFRGDDTRGGFTDHLYQAFVQNGIHTFKDDDEMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GM+++PI YKVDPSDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMEVMPIFYKVDPSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRK+LFEIA LKAWV + RS ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKSLFEIAGLKAWVSDNRSLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL--------DDNVILVGIHGMGGIGKTTIAR 240
            KP  +LIEK+++VG+TS LNQL SLLNPN         D+NVILVGIHGMGGIGK+TIAR
Sbjct: 181  KPNFTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDDNDNNVILVGIHGMGGIGKSTIAR 240

Query: 241  VCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISK 300
            VCYE IR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISK
Sbjct: 241  VCYELIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISK 300

Query: 301  AIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEE 360
            AIF KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEE
Sbjct: 301  AIFLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEE 360

Query: 361  LKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNV 420
            L+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKLVGGHPLAL+ LGSSLQNKDL VW+ V
Sbjct: 361  LEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLVGGHPLALQVLGSSLQNKDLSVWDYV 420

Query: 421  IERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA- 480
            IERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A 
Sbjct: 421  IERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAK 480

Query: 481  -NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQ 540
             N+ELLIK SL+TLS  +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I K+V  EASIQ
Sbjct: 481  RNIELLIKNSLITLSPHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KNVVTEASIQ 540

Query: 541  SIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLEL 600
            SIF K+T  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L
Sbjct: 541  SIFLKTTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPL 600

Query: 601  DSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLE 660
            +SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE
Sbjct: 601  NSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLE 660

Query: 661  DCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNG 720
             C NL NIHPSIF AK LTFLS+KDCIN++NLP  ITI  LEV ILSGC KLKK+PEF G
Sbjct: 661  GCTNLSNIHPSIFIAKKLTFLSMKDCINISNLPLQITIHNLEVFILSGCSKLKKLPEFIG 720

Query: 721  NTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGC 780
            NT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL GC
Sbjct: 721  NTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCGC 780

Query: 781  SKLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVP 840
            SKLGN T           ++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +P
Sbjct: 781  SKLGNRTRTLDGSAQRVMIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIP 840

Query: 841  EGIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKD 900
            EGIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKD
Sbjct: 841  EGIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKD 900

Query: 901  CISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSW----------MQKMFFH 960
            CISLKT   ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSW          MQK  F 
Sbjct: 901  CISLKTSTAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQWMMSGFCDMQKTAFQ 960

Query: 961  KEKFSIMIPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIP 1020
             EK SIMIPGSEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ 
Sbjct: 961  TEKLSIMIPGSEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVV 1020

Query: 1021 SLGIFASMVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCT 1080
            S  I ASM++ E   D  + ++D+ CGH  LVSGMET+DHI MLVLPWT+S         
Sbjct: 1021 SFAISASMII-EKELDGSAREEDEACGHVFLVSGMETVDHILMLVLPWTESY-------L 1080

Query: 1081 EIEFRFHMKVNRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            EIEFRF ++VNR HS+ PL + LK+CGVRLINMEE+   V WK N++LLK +F LW K
Sbjct: 1081 EIEFRFGLRVNRSHSI-PL-LGLKKCGVRLINMEEDGAAVAWKGNFILLKNSFKLWQK 1126

BLAST of Sed0012427 vs. ExPASy TrEMBL
Match: A0A6J1GPD6 (TMV resistance protein N-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455924 PE=4 SV=1)

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 794/1127 (70.45%), Postives = 911/1127 (80.83%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS  S+RLWKYDVFLSFRGEDTR  FT HLY+AF+R GI TF+DD++MEEGT+ISS
Sbjct: 1    MAASSSSLSSRLWKYDVFLSFRGEDTRGGFTGHLYEAFVRNGIHTFKDDDDMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GMK++PI YKVD SDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMKVMPIFYKVDLSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRKALFEIA LKAWV + R+ ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKALFEIAGLKAWVSDNRTLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL-------DDNVILVGIHGMGGIGKTTIARV 240
            KP L+LIEK+++VG+TS LNQL SLLNPN        D+NVILVGIHGMGGIGK+TIARV
Sbjct: 181  KPNLTLIEKEKVVGVTSKLNQLFSLLNPNTNDDDDDEDNNVILVGIHGMGGIGKSTIARV 240

Query: 241  CYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISKA 300
            CYEQIR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISKA
Sbjct: 241  CYEQIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISKA 300

Query: 301  IFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEEL 360
            I  KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEEL
Sbjct: 301  ISLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEEL 360

Query: 361  KYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVI 420
            +Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKL GGHPLAL+ LGSSLQNKDL VW+ VI
Sbjct: 361  EYEESLQLLSLNAFKERCPREGYLGQSDRIVKLAGGHPLALQVLGSSLQNKDLSVWDYVI 420

Query: 421  ERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA-- 480
            ERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A  
Sbjct: 421  ERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAKR 480

Query: 481  NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQS 540
            N+ELLI+KSL+TLSH +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I KSV  EASIQS
Sbjct: 481  NIELLIQKSLVTLSHHNRLQMHDLLQEMGRKVVPQKPVRDRLWCLKNI-KSVVTEASIQS 540

Query: 541  IFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELD 600
            IF KST  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L+
Sbjct: 541  IFLKSTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPLN 600

Query: 601  SELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLED 660
            SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE 
Sbjct: 601  SEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLEG 660

Query: 661  CPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNGN 720
            C NL NIHPSIF AK LTFLS+K+CIN+ NLP  ITI  LEV ILSGC KLKK+PEF GN
Sbjct: 661  CTNLSNIHPSIFIAKKLTFLSMKNCINITNLPLQITIHNLEVFILSGCSKLKKLPEFIGN 720

Query: 721  TQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCS 780
            T  L +L LDGT I ELPSSVASL+ L VL+L NCK LVS++ + N + SL+SLNL GCS
Sbjct: 721  TDSLLELRLDGTYIAELPSSVASLNRLTVLNLMNCKKLVSVSFSFNRISSLESLNLCGCS 780

Query: 781  KLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVPE 840
            KLGN T          T++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +PE
Sbjct: 781  KLGNRTRTSDSGAQHATIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIPE 840

Query: 841  GIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDC 900
            GIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKDC
Sbjct: 841  GIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKDC 900

Query: 901  ISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWMQKMFFHKEKFSIMIPGS 960
            ISLKT   ISKMD LY+M  +NL NCYQLAD+K IQ+LLTSWMQK  F  EK SIMIPGS
Sbjct: 901  ISLKTSTAISKMDRLYFMTGVNLQNCYQLADDKHIQTLLTSWMQKTAFQTEKLSIMIPGS 960

Query: 961  EIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVVK 1020
            EIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I ASM++ 
Sbjct: 961  EIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISASMII- 1020

Query: 1021 EPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFHMKVN 1080
            E   D  + ++D+ CGH  L SGMET+DH+WM VLP T+S         EIEFRF ++VN
Sbjct: 1021 EKELDGSAREEDEACGHVFLSSGMETVDHVWMFVLPCTESY-------LEIEFRFGLRVN 1080

Query: 1081 RYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
              HS+   +M LK+CGVRLINMEE++  V WK N++LLK +F LW K
Sbjct: 1081 PSHSIP--SMGLKKCGVRLINMEEDEAAVAWKGNFILLKNSFRLWQK 1115

BLAST of Sed0012427 vs. ExPASy TrEMBL
Match: A0A6J1GN42 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455924 PE=4 SV=1)

HSP 1 Score: 1512.7 bits (3915), Expect = 0.0e+00
Identity = 794/1137 (69.83%), Postives = 911/1137 (80.12%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS  S+RLWKYDVFLSFRGEDTR  FT HLY+AF+R GI TF+DD++MEEGT+ISS
Sbjct: 1    MAASSSSLSSRLWKYDVFLSFRGEDTRGGFTGHLYEAFVRNGIHTFKDDDDMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GMK++PI YKVD SDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMKVMPIFYKVDLSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRKALFEIA LKAWV + R+ ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKALFEIAGLKAWVSDNRTLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL-------DDNVILVGIHGMGGIGKTTIARV 240
            KP L+LIEK+++VG+TS LNQL SLLNPN        D+NVILVGIHGMGGIGK+TIARV
Sbjct: 181  KPNLTLIEKEKVVGVTSKLNQLFSLLNPNTNDDDDDEDNNVILVGIHGMGGIGKSTIARV 240

Query: 241  CYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISKA 300
            CYEQIR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISKA
Sbjct: 241  CYEQIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISKA 300

Query: 301  IFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEEL 360
            I  KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEEL
Sbjct: 301  ISLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEEL 360

Query: 361  KYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVI 420
            +Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKL GGHPLAL+ LGSSLQNKDL VW+ VI
Sbjct: 361  EYEESLQLLSLNAFKERCPREGYLGQSDRIVKLAGGHPLALQVLGSSLQNKDLSVWDYVI 420

Query: 421  ERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA-- 480
            ERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A  
Sbjct: 421  ERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAKR 480

Query: 481  NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQS 540
            N+ELLI+KSL+TLSH +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I KSV  EASIQS
Sbjct: 481  NIELLIQKSLVTLSHHNRLQMHDLLQEMGRKVVPQKPVRDRLWCLKNI-KSVVTEASIQS 540

Query: 541  IFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELD 600
            IF KST  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L+
Sbjct: 541  IFLKSTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPLN 600

Query: 601  SELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLED 660
            SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE 
Sbjct: 601  SEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLEG 660

Query: 661  CPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNGN 720
            C NL NIHPSIF AK LTFLS+K+CIN+ NLP  ITI  LEV ILSGC KLKK+PEF GN
Sbjct: 661  CTNLSNIHPSIFIAKKLTFLSMKNCINITNLPLQITIHNLEVFILSGCSKLKKLPEFIGN 720

Query: 721  TQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCS 780
            T  L +L LDGT I ELPSSVASL+ L VL+L NCK LVS++ + N + SL+SLNL GCS
Sbjct: 721  TDSLLELRLDGTYIAELPSSVASLNRLTVLNLMNCKKLVSVSFSFNRISSLESLNLCGCS 780

Query: 781  KLGNPT-------SETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVPE 840
            KLGN T          T++ LV  L++AP S VFGI S +GLYSLTEL LS+CNI+ +PE
Sbjct: 781  KLGNRTRTSDSGAQHATIKRLVLSLYRAPRSGVFGIPSMSGLYSLTELNLSNCNIETIPE 840

Query: 841  GIECLVWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDC 900
            GIE LVWLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKDC
Sbjct: 841  GIEGLVWLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKDC 900

Query: 901  ISLKTFPDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSWM----------QKMFFHK 960
            ISLKT   ISKMD LY+M  +NL NCYQLAD+K IQ+LLTSWM          QK  F  
Sbjct: 901  ISLKTSTAISKMDRLYFMTGVNLQNCYQLADDKHIQTLLTSWMQWMMSGFYDVQKTAFQT 960

Query: 961  EKFSIMIPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPS 1020
            EK SIMIPGSEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S
Sbjct: 961  EKLSIMIPGSEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVS 1020

Query: 1021 LGIFASMVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTE 1080
              I ASM++ E   D  + ++D+ CGH  L SGMET+DH+WM VLP T+S         E
Sbjct: 1021 FAISASMII-EKELDGSAREEDEACGHVFLSSGMETVDHVWMFVLPCTESY-------LE 1080

Query: 1081 IEFRFHMKVNRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            IEFRF ++VN  HS+   +M LK+CGVRLINMEE++  V WK N++LLK +F LW K
Sbjct: 1081 IEFRFGLRVNPSHSIP--SMGLKKCGVRLINMEEDEAAVAWKGNFILLKNSFRLWQK 1125

BLAST of Sed0012427 vs. ExPASy TrEMBL
Match: A0A6J1JQ43 (TMV resistance protein N-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487933 PE=4 SV=1)

HSP 1 Score: 1484.2 bits (3841), Expect = 0.0e+00
Identity = 784/1131 (69.32%), Postives = 899/1131 (79.49%), Query Frame = 0

Query: 1    MACSSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISS 60
            MA SSS SS+RLWKYDVFLSFRG+DTR  FTDHLY+AF++ GI TF+DD+EMEEGT+ISS
Sbjct: 1    MADSSSSSSSRLWKYDVFLSFRGDDTRGGFTDHLYQAFVQNGIHTFKDDDEMEEGTNISS 60

Query: 61   ELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQR 120
            EL AAIE SR AVVV+S+ YASSRWCLAELVKI EC+E  GM+++PI YKVDPSDVRNQR
Sbjct: 61   ELFAAIEASRMAVVVISEHYASSRWCLAELVKILECREHMGMEVMPIFYKVDPSDVRNQR 120

Query: 121  QSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRI 180
             SF KAF+KHE RFG++   VL WRK+LFEIA LKAWV + RS ES+  EDICEAILRRI
Sbjct: 121  GSFGKAFLKHEARFGEDDSKVLQWRKSLFEIAGLKAWVSDNRSLESKLKEDICEAILRRI 180

Query: 181  KPTLSLIEKDQLVGITSNLNQLSSLLNPNL--------DDNVILVGIHGMGGIGKTTIAR 240
            KP  +LIEK+++VG+TS LNQL SLLNPN         D+NVILVGIHGMGGIGK+TIAR
Sbjct: 181  KPNFTLIEKEKVVGVTSKLNQLFSLLNPNSNDDDDDDNDNNVILVGIHGMGGIGKSTIAR 240

Query: 241  VCYEQIREEFEVHCFLSNVREQVETSKITHIQSKLLSRITFS-KNIDIWDEEEGMVLISK 300
            VCYE IR EF+ HCF+SNVREQ ET+ ITH+QSKLLS ITFS K ID  D+ +G  LISK
Sbjct: 241  VCYELIRGEFKAHCFISNVREQFETAGITHLQSKLLSGITFSMKKIDKGDDLDGRALISK 300

Query: 301  AIFQKKTLLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEE 360
            AIF KKTLLVLDDVDH DQ+TKL P ++  F  GSR+IITTRN DLLST GVKRMFKMEE
Sbjct: 301  AIFLKKTLLVLDDVDHLDQITKLIP-DKDCFVDGSRVIITTRNADLLSTHGVKRMFKMEE 360

Query: 361  LKYKEALQLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNV 420
            L+Y+E+LQLLSLNAFKERCP+EGYLG S+ IVKLVGGHPLAL+ LGSSLQNKDL VW+ V
Sbjct: 361  LEYEESLQLLSLNAFKERCPREGYLGQSDRIVKLVGGHPLALQVLGSSLQNKDLSVWDYV 420

Query: 421  IERLQGGWSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA- 480
            IERLQGGWSID+KIFKCLKVSYDGLDDE+KEIFLD+ACFFKGK+RD+VEEIL GCD +A 
Sbjct: 421  IERLQGGWSIDDKIFKCLKVSYDGLDDEQKEIFLDIACFFKGKRRDLVEEILDGCDLYAK 480

Query: 481  -NVELLIKKSLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRDRLWGLKDINKSVGMEASIQ 540
             N+ELLIK SL+TLS  +RLQMHDLLQEMGRK+V QKPVRDRLW LK+I K+V  EASIQ
Sbjct: 481  RNIELLIKNSLITLSPHNRLQMHDLLQEMGRKVVHQKPVRDRLWCLKNI-KNVVTEASIQ 540

Query: 541  SIFFKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLEL 600
            SIF K+T  +V IP+  SRMHQLRL+NF NV+LQ+QSE CIPSELRYLKWKRYP E L L
Sbjct: 541  SIFLKTTGKIVLIPLSLSRMHQLRLLNFHNVRLQDQSECCIPSELRYLKWKRYPFEFLPL 600

Query: 601  DSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLE 660
            +SE++Y LI+LHMC SNLKQ WHGEK LEKL+YIKV HSQ L++TPNFTQLPNL+ LDLE
Sbjct: 601  NSEVDYNLIQLHMCYSNLKQLWHGEKNLEKLKYIKVGHSQKLFKTPNFTQLPNLERLDLE 660

Query: 661  DCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLKLKKIPEFNG 720
             C NL NIHPSIF AK LTFLS+KDCIN++NLP  ITI  LEV ILSGC KLKK+PEF G
Sbjct: 661  GCTNLSNIHPSIFIAKKLTFLSMKDCINISNLPLQITIHNLEVFILSGCSKLKKLPEFIG 720

Query: 721  NTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGC 780
            NT  L +L LDGTSI ELPSSVASL+ L VL+L NCK LVS+T + N L SL+SLNL GC
Sbjct: 721  NTDSLLELRLDGTSIAELPSSVASLNRLTVLNLMNCKKLVSVTFSFNRLSSLESLNLCGC 780

Query: 781  SKLGNPTSETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNIDVVPEGIECLV 840
            SKLGN T   TL+    R+                      +I  +CNI+ +PEGIE LV
Sbjct: 781  SKLGNRT--RTLDGSAQRV----------------------MIKRNCNIETIPEGIEGLV 840

Query: 841  WLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLKTF 900
            WLR+LNLSNNSF ELP  +A+LHNLR LN+NGCKKLVQVPELP RIMKLKMKDCISLKT 
Sbjct: 841  WLRMLNLSNNSFTELPASLAQLHNLRSLNMNGCKKLVQVPELPARIMKLKMKDCISLKTS 900

Query: 901  PDISKMDHLYYMGEMNLINCYQLADNKQIQSLLTSW----------MQKMFFHKEKFSIM 960
              ISKMD LY+M E+NL+NCYQLAD+K IQ+LLTSW          MQK  F  EK SIM
Sbjct: 901  TAISKMDRLYFMTEVNLLNCYQLADDKHIQTLLTSWMQWMMSGFCDMQKTAFQTEKLSIM 960

Query: 961  IPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFAS 1020
            IPGSEIP WFTTTK GSSIS+KWDPN  N N+ICFALCVV GPT  Q   T+ S  I AS
Sbjct: 961  IPGSEIPDWFTTTKMGSSISMKWDPNARNGNKICFALCVVFGPTHPQNITTVVSFAISAS 1020

Query: 1021 MVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLPWTKSMWDEINKCTEIEFRFH 1080
            M++ E   D  + ++D+ CGH  LVSGMET+DHI MLVLPWT+S         EIEFRF 
Sbjct: 1021 MII-EKELDGSAREEDEACGHVFLVSGMETVDHILMLVLPWTESY-------LEIEFRFG 1080

Query: 1081 MKVNRYHSVNPLNMELKRCGVRLINMEEEKQNVEWKENYMLLKTAFNLWAK 1109
            ++VNR HS+ PL + LK+CGVRLINMEE+   V WK N++LLK +F LW K
Sbjct: 1081 LRVNRSHSI-PL-LGLKKCGVRLINMEEDGAAVAWKGNFILLKNSFKLWQK 1095

BLAST of Sed0012427 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 494.6 bits (1272), Expect = 2.1e-139
Identity = 336/916 (36.68%), Postives = 518/916 (56.55%), Query Frame = 0

Query: 4   SSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELL 63
           SSS SS+ +WK DVF+SFRGED R  F  HL+  F R GI  FRDD +++ G  IS EL+
Sbjct: 7   SSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELI 66

Query: 64  AAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSF 123
            AI+ SR A+VVVS++YA+S WCL EL+KI EC +     I+PI Y+VDPSDVR QR SF
Sbjct: 67  DAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKD---TIVPIFYEVDPSDVRRQRGSF 126

Query: 124 KKAFVKH--EKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRIK 183
            +    H  +++ GK       W++AL ++A++     +    +S+ I+ I + I  ++ 
Sbjct: 127 GEDVESHSDKEKVGK-------WKEALKKLAAISG-EDSRNWDDSKLIKKIVKDISDKLV 186

Query: 184 PTLSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREE 243
            T S  +   L+G++S+++ L S+++  +D +V ++GI GMGG+GKTTIA+  Y Q+  +
Sbjct: 187 ST-SWDDSKGLIGMSSHMDFLQSMIS-IVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQ 246

Query: 244 FEVHCFLSNVREQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLV 303
           F+VHCF+ NV+E      +  +Q + L R+   ++ + W       +I +    K   +V
Sbjct: 247 FQVHCFMENVKEVCNRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIV 306

Query: 304 LDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLL 363
           LDDVD  +Q+ +L  K  G+FG GSRII+TTR+  LL + G+  ++K++ L  KEALQL 
Sbjct: 307 LDDVDRSEQLNELV-KETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLF 366

Query: 364 SLNAFKER-CPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWS 423
              AF+E      G+   S   V    G PLAL+ LGS L  +    W + + RL+    
Sbjct: 367 CNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPH 426

Query: 424 IDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHA--NVELLIKK 483
            D  I + L+VSYDGLD++EK IFL ++CF+  K+ D V ++L  C + A   + +L +K
Sbjct: 427 SD--IMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEK 486

Query: 484 SLLTLSHDSRLQMHDLLQEMGRKIVCQKPV-----RDRLWGLKDINKSVGMEASIQ---- 543
           SL+  S+   +++HDLL++MGR++V Q+ V     R  LW  +DI   +   +  Q    
Sbjct: 487 SLIVESNGC-VKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEG 546

Query: 544 -SIFFKSTRNVVPIPIMFSRMHQLRLINF--------RNVKLQNQSEYCIPSELRYLKWK 603
            S+       V      F  +  L+L+NF          V L N   Y +P +LRYL+W 
Sbjct: 547 ISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSY-LPRKLRYLRWD 606

Query: 604 RYPMEVLELDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQTPNFTQL 663
            YP++ +      E+ L+EL M +SNL++ W G + L  L+ + +S  + L + P+ ++ 
Sbjct: 607 GYPLKTMPSRFFPEF-LVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKA 666

Query: 664 PNLQILDLEDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVLILSGCLK 723
            NL+ L+L  C +L+ + PSI   K L+   L +CI L ++P  I ++ LE + +SGC  
Sbjct: 667 TNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSS 726

Query: 724 LKKIPEFNGNTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPS 783
           LK  PE + NT+R   LYL  T I ELPSS++ L CL  L + +C+ L ++ S    L S
Sbjct: 727 LKHFPEISWNTRR---LYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVS 786

Query: 784 LKSLNLSGCSKLGNPTSETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELI-LSDCNID 843
           LKSLNL GC +L N     TL+ L S L     S    +  F  + +  E++ +S+ +I+
Sbjct: 787 LKSLNLDGCRRLEN--LPDTLQNLTS-LETLEVSGCLNVNEFPRVSTSIEVLRISETSIE 846

Query: 844 VVPEGIECLVWLRVLNLSNNS-FCELPPCIAKLHNLRGLNINGCKKLVQVP-ELPERIMK 892
            +P  I  L  LR L++S N     LP  I++L +L  L ++GC  L   P E+ + +  
Sbjct: 847 EIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSC 897

BLAST of Sed0012427 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 488.0 bits (1255), Expect = 2.0e-137
Identity = 385/1192 (32.30%), Postives = 598/1192 (50.17%), Query Frame = 0

Query: 4    SSSLSSTRLWKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELL 63
            SSS SS    ++DVFLSFRG DTR NFT HL KA   +GI +F DD  +  G ++++ L 
Sbjct: 3    SSSPSSA---EFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLTA-LF 62

Query: 64   AAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRNQRQSF 123
              IE S+ A++V S +YA+S WCL ELVKI EC+      ++PI YKVD SDV  QR SF
Sbjct: 63   DRIEKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSF 122

Query: 124  KKAFVKHEKRF-GKNSLNVLSWRKALFEIASLKAWVLNERS-HESEFIEDICEAILRRIK 183
               F   E  F G     + SW+ AL   +++  +V+ E S  E++ +++I     +++ 
Sbjct: 123  AVPFKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN 182

Query: 184  PTLSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREE 243
              L+    + LVGI S L  L  LL+    D V ++GI GM GIGKTT+A   Y ++R +
Sbjct: 183  -DLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQ 242

Query: 244  FEVHCFLSNVREQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLV 303
            F+  CFL+N+RE    S +  +  KL S +   ++++I           + +  K+ L+V
Sbjct: 243  FDGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIV 302

Query: 304  LDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLL 363
            LDDV+   Q+  L    + Y G GSRIIITTR+  L+ T+   R + + +L  +EAL+L 
Sbjct: 303  LDDVNDEKQIRYLMGHCKWYQG-GSRIIITTRDSKLIETI-KGRKYVLPKLNDREALKLF 362

Query: 364  SLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWSI 423
            SLNAF    P + + G +N ++    GHPLALK LGS L  +D   W   ++RL+     
Sbjct: 363  SLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHG 422

Query: 424  DEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEIL--VGCDFHANVELLIKKS 483
            D  I++ L+ SY+ L  E+K +FLD+ACFF+ +  D V  +L   G D    V+ L+ K 
Sbjct: 423  D--IYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKC 482

Query: 484  LLTLSHDSRLQMHDLLQEMGRKIVCQKP---VRD---------------RLWGLKDI--- 543
            L+TLS D+R++MHD+LQ M ++I  +     +RD               RLW  +DI   
Sbjct: 483  LITLS-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDL 542

Query: 544  -NKSVGMEASIQSIFFKST--RNVVPIPIMFSRMHQLRLINFRNVKLQNQSE-------- 603
              + +G +  I+ IF  ++  R +      F  M+ L+ +   +       E        
Sbjct: 543  LTEGLGTD-KIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLR 602

Query: 604  ---YCIPSELRYLKWKRYPMEVLELDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIK 663
                 +P+EL YL W  YP++ + LD + +  L++L +  S L++ W  EK +  L+++ 
Sbjct: 603  RGLSFLPNELTYLHWHGYPLQSIPLDFDPK-NLVDLKLPHSQLEEIWDDEKDVGMLKWVD 662

Query: 664  VSHSQNLYQTPNFTQLPNLQILDLEDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGH 723
            +SHS NL Q        NL+ L+LE C +L  +  +I   + L +L+L+DC +L +LP  
Sbjct: 663  LSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKG 722

Query: 724  ITIEILEVLILSGCLKLKKIPEFNGNTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKN 783
            I  + L+ LILSGC  LKK P  + N   ++ L LDGT I  LP S+ +   L +L+LKN
Sbjct: 723  IKTQSLQTLILSGCSSLKKFPLISEN---VEVLLLDGTVIKSLPESIQTFRRLALLNLKN 782

Query: 784  CKMLVSITSATNWLPSLKSLNLSGCSKLG-----------------NPTSETTLEWLVSR 843
            CK L  ++S    L  L+ L LSGCS+L                  + TS T +  ++  
Sbjct: 783  CKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMM-H 842

Query: 844  LFKAPTSRVFGIASFA-----------GLYSLTELILSDCNIDVVPEGIECLVWLRVLNL 903
            L    T  + G +S             G   LT+L LS C++  +P+ I  L  L+ L L
Sbjct: 843  LSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCL 902

Query: 904  SNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLKTFPD----- 963
            S N+   LP    +L+NL+  ++  CK L  +P LP+ +  L   +C SL+T  +     
Sbjct: 903  SGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPL 962

Query: 964  -ISKMDHLYYMGEMNLINCYQLADNKQI---------QSLLTSWMQKMFFH----KEKFS 1023
             + +  H  ++      NCY+L  + Q            L+ +   K ++     +    
Sbjct: 963  TVGERIHSMFI----FSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVG 1022

Query: 1024 IMIPGSEIPSWFTTTKTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNG---TIPSL 1083
            I  P +EIPSWF   + G S+ I   P+  + N +  AL VV    D + +    ++   
Sbjct: 1023 ICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCC 1082

Query: 1084 GIFASMVVKEPNHDLDSEDDDDVCGHGILVSGMETLDHIWMLVLP--WTKSMWDEINKC- 1101
            G F +        D      ++ CG     S   T DH++M        K++  E N C 
Sbjct: 1083 GNFENKDSSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCC 1142

BLAST of Sed0012427 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 488.0 bits (1255), Expect = 2.0e-137
Identity = 352/1008 (34.92%), Postives = 533/1008 (52.88%), Query Frame = 0

Query: 13  WKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELLAAIEVSRAA 72
           W YDVF+SFRG D R NF  HLY +  R GI TF DD E++ G  IS ELL AIE S+  
Sbjct: 12  WTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKIL 71

Query: 73  VVVVSKDYASSRWCLAELVKIFEC-KEQKGMKIIPIIYKVDPSDVRNQRQSFKKAFVKHE 132
           +VV++KDYASS WCL ELV I +  K      + PI   VDPSD+R Q+ S+ K+F KH+
Sbjct: 72  IVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHK 131

Query: 133 KRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRIKPTLSLIEKDQ 192
                N L    WR+AL ++A++  W +  R +E+E I DI   IL+R+ P   L     
Sbjct: 132 NSHPLNKLK--DWREALTKVANISGWDIKNR-NEAECIADITREILKRL-PCQYLHVPSY 191

Query: 193 LVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREEFEVHCFLSNV 252
            VG+ S L  +SSLL+    D V ++ I+GMGGIGKTT+A+V + +    FE   FL N 
Sbjct: 192 AVGLRSRLQHISSLLSIG-SDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENF 251

Query: 253 REQVETSK-ITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLVLDDVDHFDQ 312
           RE  +  +  TH+Q +LLS I    +I+    +     + +    K+ LLV+DDVD   Q
Sbjct: 252 REYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQ 311

Query: 313 MTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLLSLNAFKERC 372
           +       +  FG+GSRIIITTRN  LL  L  +  +  +EL   E+L+L S +AF+   
Sbjct: 312 LNSAAIDRD-CFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSE 371

Query: 373 PKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWSIDEKIFKCLK 432
           P + +L HS  +V    G PLA++ LG+ L  + +  W + ++ L+     ++ I   L+
Sbjct: 372 PPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKR--IPNDNIQAKLQ 431

Query: 433 VSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHANV--ELLIKKSLLTLSHDSR 492
           +S++ L  E+K++FLD+ACFF G     V  IL GC+ + ++   LL+++ L+T+S  + 
Sbjct: 432 ISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS-GNN 491

Query: 493 LQMHDLLQEMGRKIVCQ-KP----VRDRLWGLKDI----NKSVGMEASIQSIFFKSTRNV 552
           + MHDLL++MGR+IV +  P     R RLW   D+     K  G  A I+ +  K+  +V
Sbjct: 492 IMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNA-IEGLSLKA--DV 551

Query: 553 VPIPIM----FSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELDSELEY 612
           +         F++M +LRL+  R V L    E+  P +LR+L W  + +E   ++  LE 
Sbjct: 552 MDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH-FPKDLRWLCWHGFSLECFPINLSLE- 611

Query: 613 KLIELHMCSSNLKQFWHGE---KYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLEDCP 672
            L  L +  SNLK+FW  +   +    ++Y+ +SHS  L +TP+F+  PN++ L L +C 
Sbjct: 612 SLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCK 671

Query: 673 NLINIHPSI-FTAKSLTFLSLKDCINLNNLPGHI-TIEILEVLILSGCLKLKKIPEFNGN 732
           +L+ +H SI    K L  L+L  CI L+ LP  I  ++ LE L LS C KL+++ +  G 
Sbjct: 672 SLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGE 731

Query: 733 TQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCS 792
            + L  L  D T++ E+PS++  L  L+ LSL  CK L+S                    
Sbjct: 732 LESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLS-------------------D 791

Query: 793 KLGNPTSETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNI--DVVPEGIECL 852
            + N  SE           K+ +  +    S +GL  +  L L  CN+  +++PE I  L
Sbjct: 792 DIDNLYSE-----------KSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSL 851

Query: 853 VWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLKT 912
            +LR L+L  NSFC LP   A L NL  L ++ C KL  +  LP  ++ L +  CI LK 
Sbjct: 852 SFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLLFLDVGKCIMLKR 911

Query: 913 FPDISKMDHLY--------------------YMGEMNLINCYQLADNKQIQSLLTSWMQK 972
            PDISK   L+                    Y+  + L  C   + +  I ++L +W+++
Sbjct: 912 TPDISKCSALFKLQLNDCISLFEIPGIHNHEYLSFIVLDGCKLASTDTTINTMLENWLKR 970

Query: 973 MFFHKEKFSIMIPGSEIPSW--FTTTKTGSSISIKWDPNNANQICFAL 975
              H+  +  +   + IP+W  F   K   SI++    N+   + F L
Sbjct: 972 N--HECIYIPVDRPNVIPNWVYFEEEKRSFSITVPETDNSDTVVGFTL 970

BLAST of Sed0012427 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 488.0 bits (1255), Expect = 2.0e-137
Identity = 352/1008 (34.92%), Postives = 533/1008 (52.88%), Query Frame = 0

Query: 13  WKYDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDISSELLAAIEVSRAA 72
           W YDVF+SFRG D R NF  HLY +  R GI TF DD E++ G  IS ELL AIE S+  
Sbjct: 15  WTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKIL 74

Query: 73  VVVVSKDYASSRWCLAELVKIFEC-KEQKGMKIIPIIYKVDPSDVRNQRQSFKKAFVKHE 132
           +VV++KDYASS WCL ELV I +  K      + PI   VDPSD+R Q+ S+ K+F KH+
Sbjct: 75  IVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHK 134

Query: 133 KRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILRRIKPTLSLIEKDQ 192
                N L    WR+AL ++A++  W +  R +E+E I DI   IL+R+ P   L     
Sbjct: 135 NSHPLNKLK--DWREALTKVANISGWDIKNR-NEAECIADITREILKRL-PCQYLHVPSY 194

Query: 193 LVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQIREEFEVHCFLSNV 252
            VG+ S L  +SSLL+    D V ++ I+GMGGIGKTT+A+V + +    FE   FL N 
Sbjct: 195 AVGLRSRLQHISSLLSIG-SDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENF 254

Query: 253 REQVETSK-ITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKTLLVLDDVDHFDQ 312
           RE  +  +  TH+Q +LLS I    +I+    +     + +    K+ LLV+DDVD   Q
Sbjct: 255 REYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQ 314

Query: 313 MTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEALQLLSLNAFKERC 372
           +       +  FG+GSRIIITTRN  LL  L  +  +  +EL   E+L+L S +AF+   
Sbjct: 315 LNSAAIDRD-CFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSE 374

Query: 373 PKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGGWSIDEKIFKCLK 432
           P + +L HS  +V    G PLA++ LG+ L  + +  W + ++ L+     ++ I   L+
Sbjct: 375 PPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKR--IPNDNIQAKLQ 434

Query: 433 VSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHANV--ELLIKKSLLTLSHDSR 492
           +S++ L  E+K++FLD+ACFF G     V  IL GC+ + ++   LL+++ L+T+S  + 
Sbjct: 435 ISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS-GNN 494

Query: 493 LQMHDLLQEMGRKIVCQ-KP----VRDRLWGLKDI----NKSVGMEASIQSIFFKSTRNV 552
           + MHDLL++MGR+IV +  P     R RLW   D+     K  G  A I+ +  K+  +V
Sbjct: 495 IMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNA-IEGLSLKA--DV 554

Query: 553 VPIPIM----FSRMHQLRLINFRNVKLQNQSEYCIPSELRYLKWKRYPMEVLELDSELEY 612
           +         F++M +LRL+  R V L    E+  P +LR+L W  + +E   ++  LE 
Sbjct: 555 MDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH-FPKDLRWLCWHGFSLECFPINLSLE- 614

Query: 613 KLIELHMCSSNLKQFWHGE---KYLEKLQYIKVSHSQNLYQTPNFTQLPNLQILDLEDCP 672
            L  L +  SNLK+FW  +   +    ++Y+ +SHS  L +TP+F+  PN++ L L +C 
Sbjct: 615 SLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCK 674

Query: 673 NLINIHPSI-FTAKSLTFLSLKDCINLNNLPGHI-TIEILEVLILSGCLKLKKIPEFNGN 732
           +L+ +H SI    K L  L+L  CI L+ LP  I  ++ LE L LS C KL+++ +  G 
Sbjct: 675 SLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGE 734

Query: 733 TQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITSATNWLPSLKSLNLSGCS 792
            + L  L  D T++ E+PS++  L  L+ LSL  CK L+S                    
Sbjct: 735 LESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLS-------------------D 794

Query: 793 KLGNPTSETTLEWLVSRLFKAPTSRVFGIASFAGLYSLTELILSDCNI--DVVPEGIECL 852
            + N  SE           K+ +  +    S +GL  +  L L  CN+  +++PE I  L
Sbjct: 795 DIDNLYSE-----------KSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSL 854

Query: 853 VWLRVLNLSNNSFCELPPCIAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLKT 912
            +LR L+L  NSFC LP   A L NL  L ++ C KL  +  LP  ++ L +  CI LK 
Sbjct: 855 SFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLLFLDVGKCIMLKR 914

Query: 913 FPDISKMDHLY--------------------YMGEMNLINCYQLADNKQIQSLLTSWMQK 972
            PDISK   L+                    Y+  + L  C   + +  I ++L +W+++
Sbjct: 915 TPDISKCSALFKLQLNDCISLFEIPGIHNHEYLSFIVLDGCKLASTDTTINTMLENWLKR 973

Query: 973 MFFHKEKFSIMIPGSEIPSW--FTTTKTGSSISIKWDPNNANQICFAL 975
              H+  +  +   + IP+W  F   K   SI++    N+   + F L
Sbjct: 975 N--HECIYIPVDRPNVIPNWVYFEEEKRSFSITVPETDNSDTVVGFTL 973

BLAST of Sed0012427 vs. TAIR 10
Match: AT4G19510.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 439.9 bits (1130), Expect = 6.2e-123
Identity = 357/1170 (30.51%), Postives = 561/1170 (47.95%), Query Frame = 0

Query: 1    MACSSSLSSTRLWK--YDVFLSFRGEDTRVNFTDHLYKAFIRKGIVTFRDDNEMEEGTDI 60
            MA +SS SS  L K  +DVF+SFRG DTR +FT HL K    KGI  F D      G + 
Sbjct: 41   MAFASSSSSIVLSKCEFDVFVSFRGADTRHDFTSHLVKYLRGKGIDVFSD--AKLRGGEY 100

Query: 61   SSELLAAIEVSRAAVVVVSKDYASSRWCLAELVKIFECKEQKGMKIIPIIYKVDPSDVRN 120
             S L   IE S+ ++VV S+DYA+S WCL E+ KI + +++    ++PI YKV  SDV N
Sbjct: 101  ISLLFDRIEQSKMSIVVFSEDYANSWWCLEEVGKIMQRRKEFNHGVLPIFYKVSKSDVSN 160

Query: 121  QRQSFKKAFVKHEKRFGKNSLNVLSWRKALFEIASLKAWVLNERSHESEFIEDICEAILR 180
            Q  SF+  F    K F  +   +   + AL   ++++ +V  E S E +F+++I +   R
Sbjct: 161  QTGSFEAVFQSPTKIFNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTFR 220

Query: 181  RIKPTLSLIEKDQLVGITSNLNQLSSLLNPNLDDNVILVGIHGMGGIGKTTIARVCYEQI 240
             +      +  D L GI S   +L  LL  + D+ V +VG+ GM GIGKTT+A + Y+Q 
Sbjct: 221  MLNELSPCVIPDDLPGIESRSKELEKLLMFDNDECVRVVGVLGMTGIGKTTVADIVYKQN 280

Query: 241  REEFEVHCFLSNVREQVETSKITHIQSKLLSRITFSKNIDIWDEEEGMVLISKAIFQKKT 300
             + F+ + FL ++ +  +   + ++  KLL ++   +N+D+    +G       +  KK 
Sbjct: 281  FQRFDGYEFLEDIEDNSKRYGLPYLYQKLLHKLLDGENVDV--RAQGRP--ENFLRNKKL 340

Query: 301  LLVLDDVDHFDQMTKLTPKNEGYFGYGSRIIITTRNEDLLSTLGVKRMFKMEELKYKEAL 360
             +VLD+V    Q+  L  K   Y   GSRI+I TR++ LL        + +  L  +EA+
Sbjct: 341  FIVLDNVTEEKQIEYLIGKKNVY-RQGSRIVIITRDKKLLQK-NADATYVVPRLNDREAM 400

Query: 361  QLLSLNAFKERCPKEGYLGHSNTIVKLVGGHPLALKTLGSSLQNKDLYVWNNVIERLQGG 420
            +L  L  F    P E ++  SN  V    G PLALK LG  L   D+  W   +E LQ  
Sbjct: 401  ELFCLQVFGNHYPTEEFVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQ-- 460

Query: 421  WSIDEKIFKCLKVSYDGLDDEEKEIFLDVACFFKGKKRDVVEEILVGCDFHANVELLIKK 480
             + D+++ K LK SY  LDD++K +FLD+ACFF                           
Sbjct: 461  VNPDKELQKELKSSYKALDDDQKSVFLDIACFF--------------------------- 520

Query: 481  SLLTLSHDSRLQMHDLLQEMGRKIVCQKPVRD-----RLWGLKDI----NKSVGMEASIQ 540
                     R++MHDLL  MG++I  +K +R      RLW  KDI      + G E  ++
Sbjct: 521  ---------RIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTEC-VR 580

Query: 541  SIF--FKSTRNVVPIPIMFSRMHQLRLINFRNVKLQNQSEYC--------------IPSE 600
             IF      R +   P  F+ + +L+ + F +    + S++C               P E
Sbjct: 581  GIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHS---SHCSQWCDNDHIFQCSKVPDHFPDE 640

Query: 601  LRYLKWKRYPMEVLELDSELEYKLIELHMCSSNLKQFWHGEKYLEKLQYIKVSHSQNLYQ 660
            L YL W+ YP + L  D + + +L++L +  S++KQ W  EK  E L+++ +  S++L  
Sbjct: 641  LVYLHWQGYPYDCLPSDFDPK-ELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 700

Query: 661  TPNFTQLPNLQILDLEDCPNLINIHPSIFTAKSLTFLSLKDCINLNNLPGHITIEILEVL 720
                ++  NL+ LDLE C +L ++  S+     L +L+L+DC +L +LP    I+ L+ L
Sbjct: 701  LSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTL 760

Query: 721  ILSGCLKLKKIPEFNGNTQRLQQLYLDGTSITELPSSVASLDCLRVLSLKNCKMLVSITS 780
            ILSGCLKLK   +F+  ++ ++ L+L+GT+I  +   + SL  L +L+LKNC+ L  + +
Sbjct: 761  ILSGCLKLK---DFHIISESIESLHLEGTAIERVVEHIESLHSLILLNLKNCEKLKYLPN 820

Query: 781  ATNWLPSLKSLNLSGCSKL-------------------GNPTSETTLEWLVSRL----FK 840
                L SL+ L LSGCS L                   G    +T     +S L    F 
Sbjct: 821  DLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMDGTSIKQTPEMSCLSNLKICSFC 880

Query: 841  APT---SRVFGIASFAGLYSLTELILSDCNIDVVPEGIECLVWLRVLNLSNNSFCELPPC 900
             P    S    +  F+G   L++L L++CNID +P+    L  LR L LS N+   LP  
Sbjct: 881  RPVIDDSTGLVVLPFSGNSFLSDLYLTNCNIDKLPDKFSSLRSLRCLCLSRNNIETLPES 940

Query: 901  IAKLHNLRGLNINGCKKLVQVPELPERIMKLKMKDCISLK------TFPDISKMDHLYYM 960
            I KL++L  L++  C +L  +P LP  +  L    C SL+      T P +++  H  ++
Sbjct: 941  IEKLYSLLLLDLKHCCRLKSLPLLPSNLQYLDAHGCGSLENVSKPLTIPLVTERMHTTFI 1000

Query: 961  ----GEMNLINCYQLADNKQIQSLLTSWMQKMFFHK-----EKFSIMIPGSEIPSWFTTT 1020
                 ++N      +    Q++S L +   +   HK        ++  PG +IPSWF+  
Sbjct: 1001 FTDCFKLNQAEKEDIVAQAQLKSQLLARTSRHHNHKGLLLDPLVAVCFPGHDIPSWFSHQ 1060

Query: 1021 KTGSSISIKWDPN--NANQICFALCVVCGPTDQQQNGTIPSLGIFASMVVKEPNHDLDSE 1080
            K GS I     P+  N+  I  +LCVV    D + +     L +      K  N    S 
Sbjct: 1061 KMGSLIETDLLPHWCNSKFIGASLCVVVTFKDHEGHHA-NRLSVRCKSKFKSQNGQFISF 1120

Query: 1081 DD-----DDVCGHGILVSGMETLDHIWML-------VLPWTKSMWDEINKC--TEIEFRF 1087
                   ++ CG           DH+++        V  W++   +E N+C  T   F F
Sbjct: 1121 SFCLGGWNESCGSSCHEPRKLGSDHVFISYNNCNVPVFKWSEET-NEGNRCHPTSASFEF 1151