Sed0012357 (gene) Chayote v1

BLAST of Sed0012357 vs. NCBI nr
Match: XP_022931496.1 (subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata])

HSP 1 Score: 981.1 bits (2535), Expect = 5.4e-282
Identity = 502/765 (65.62%), Postives = 612/765 (80.00%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+ +SF+   +  +A+FS      +A++D+QN KVYI YLGER Y+D KLTT SHH+LL 
Sbjct: 4   NRASSFMHAVVTIYAVFS-----AMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +V+GSKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRSWD
Sbjct: 64  SVMGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++G+
Sbjct: 124 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG +P
Sbjct: 244 YFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L+
Sbjct: 304 LPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLT 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D+ 
Sbjct: 364 SITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFIDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++ K
Sbjct: 424 MKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSDEK 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  D 
Sbjct: 484 ALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+PPK
Sbjct: 544 GFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FNS 
Sbjct: 664 ASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. NCBI nr
Match: XP_022931497.1 (subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata])

HSP 1 Score: 979.9 bits (2532), Expect = 1.2e-281
Identity = 503/765 (65.75%), Postives = 612/765 (80.00%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+ +SF+   +  +A+FS      +A++D+QN KV+IVYLGER YDD KLTT SHH+LL 
Sbjct: 4   NRASSFMHAVVTIYAVFS-----AMAEADNQNSKVHIVYLGERPYDDVKLTTHSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +VL SKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRSWD
Sbjct: 64  SVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++G+
Sbjct: 124 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG +P
Sbjct: 244 YFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L+
Sbjct: 304 LPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLT 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D+ 
Sbjct: 364 SITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFIDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++ K
Sbjct: 424 MKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSDEK 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  D 
Sbjct: 484 ALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+PPK
Sbjct: 544 GFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FNS 
Sbjct: 664 ASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. NCBI nr
Match: XP_022931495.1 (subtilisin-like protease SBT3.7 isoform X3 [Cucurbita moschata])

HSP 1 Score: 978.8 bits (2529), Expect = 2.7e-281
Identity = 503/767 (65.58%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 1   MVNKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDL 60
           M N QA   +  +    ++++F+   +A+ DDQN KV+IVYLGER YDD KLTT SHH+L
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFS--AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSHHEL 60

Query: 61  LTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRS 120
           L +VL SKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRS
Sbjct: 61  LESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRS 120

Query: 121 WDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEV 180
           WD+LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++
Sbjct: 121 WDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQL 180

Query: 181 GQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTK 240
           G+ FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  
Sbjct: 181 GEDFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRN 240

Query: 241 VSFYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGF 300
           VS++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG 
Sbjct: 241 VSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGS 300

Query: 301 LPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRAL 360
           +PL P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA 
Sbjct: 301 IPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAF 360

Query: 361 LSSI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLD 420
           L+SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D
Sbjct: 361 LTSITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFID 420

Query: 421 VVDMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLAN 480
           +   +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++
Sbjct: 421 LAMKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSD 480

Query: 481 SKPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFS 540
            K ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  
Sbjct: 481 EKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGG 540

Query: 541 DNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNP 600
           D GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+P
Sbjct: 541 DKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSP 600

Query: 601 PKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPS 660
           PK+ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS
Sbjct: 601 PKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPS 660

Query: 661 LKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFN 720
            + S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FN
Sbjct: 661 KRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFN 720

Query: 721 SKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           S +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 721 SNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Sed0012357 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 978.0 bits (2527), Expect = 4.6e-281
Identity = 500/765 (65.36%), Postives = 605/765 (79.08%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+  SFIL  +  +A+FS      +A++DDQN KV+IVYLGER YDD KLTT SHH+LL 
Sbjct: 4   NRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +VLGSKEKSLESMVYSY+HGFSGFAAKLT S AQKLA MP V +VF SS+YK +TTRSWD
Sbjct: 64  SVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNL HRAKMGDNVIIG+ID+GFWPES+SF D+G+GPIPSRWKG C+ G+
Sbjct: 124 FLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS++CNKKVIGARWF+   IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G+ ADILK IDEAIHDGVDVLSMSIG  +P
Sbjct: 244 YFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L+P+ N+ N +A+GSFHA+A+GI VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L 
Sbjct: 304 LFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLV 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISM-GRCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ F   +KD+V  L++M GRC   LG +   I      ++LCF D+ 
Sbjct: 364 SITTLGDNATYLGQMFL--RKDIVGMLVAMDGRCAGILGGD---IPISGNVVLLCFTDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +    A +   +    G+I AGQQ + L P CD    CI VDT VGTKL  Y L +  
Sbjct: 424 KTAPASNAVMPGKQAKVVGVIYAGQQTDNLVP-CD--IPCIHVDTHVGTKLFTYLLNDDD 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R++  +TIIG PIS+RIAYFSSRGPNS SP ILKPDIAAPG+NI+AAVPP+ +  D 
Sbjct: 484 ALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+ L+K +HPTWSPAAIKSALITTARV+DPSG+PIF++G+PPK
Sbjct: 544 GFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +Y+C MGY   DIS L+QQKT CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPAL NSTTVTRTVTNVGNLT++Y+AV++AP G KV VKPRVL FNS 
Sbjct: 664 ASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 975.7 bits (2521), Expect = 2.3e-280
Identity = 508/770 (65.97%), Postives = 601/770 (78.05%), Query Frame = 0

Query: 2   VNKQ-ASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDL 61
           +N Q A  I+ FI F+ L        LA+S+DQNPKV+IVYLGE+ + D+K TT SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRS 121
           L  +LGSKEKSLE+MVYSYKHGFSGFAAKLTKS AQKL+EM  V++V  SS+YK  TTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEV 181
           WD+LGLSSSP  SSNLLHRAK GD+VIIG+IDSG WPES+SF+D+GLGPIPSRWKG CE 
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTK 241
           G+QFNSTNCNKK+IGARWF K F+ADYG+E L  EYLS RD++ HGTHTASTAAGSFV  
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 VSFYGQATGTVRGGAPLARLAIYKVQW--NGVGTSADILKAIDEAIHDGVDVLSMSIGGF 301
           ++++  A GT RGGAPLARLAIYK  W   GVG+SADILKAIDEAIHDGVDVLS+SIGG 
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 LPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRAL 361
            P YPE  E + IA GSFHA+ +GI VVCA GN GP  Q V N APWI TVA +T+DRA 
Sbjct: 303 PPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAF 362

Query: 362 LSSI-SLGDNTTYFGQSFFNSKKDLVTELISMGRCDKNLGNESSIIQFIRGKLVLCFLDV 421
           LSSI +L DNTT+ GQS   SKKDLV EL+S  RCD+  GNE+    FI GK+VLCF  +
Sbjct: 363 LSSITTLRDNTTFMGQSLLESKKDLVAELVSWERCDQLSGNEA----FINGKVVLCFPKL 422

Query: 422 VDMSIIGLAGLAVVKQYGSGIIVAGQQ--NNILEPRCDDS-FACIIVDTDVGTKLHFYTL 481
            D S I  A   VV+  G+GIIVAGQQ  NN+L   C  S   CI+VDT VG+KL FY L
Sbjct: 423 ADYSTISKAAEVVVRANGTGIIVAGQQVDNNLL--ACISSPIPCILVDTIVGSKLFFYFL 482

Query: 482 ANS-KPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHA 541
            NS  P+V ++ A+TIIG PI+  IA+FSSRGPNS SP ILKPDI+APGSNIL+AV PH 
Sbjct: 483 QNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHY 542

Query: 542 EFSDNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSD 601
            F++ GF + SGTSM+TPHV+ I+ALLK VHPTWSPAAIKSAL+TTAR +   GLPIF+D
Sbjct: 543 FFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAD 602

Query: 602 GNPPKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTK 661
           G PPK+ADPFDYG GVVD NAA+DPGLIY++   DYI +Y+CGMGY+  DIS LT +KT+
Sbjct: 603 GTPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTE 662

Query: 662 CPSLKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVL 721
           CP  + S+L+LNLP IT+P+L NST VTRTVTNVGNL+ +Y+A +EAP G KVSV P+VL
Sbjct: 663 CPLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVL 722

Query: 722 AFNSKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
            FNS++KKISFKV   T   +N+GYSFG LTWSDGVH+VK PLS++  FF
Sbjct: 723 VFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGFF 761

BLAST of Sed0012357 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 742.3 bits (1915), Expect = 5.6e-213
Identity = 385/743 (51.82%), Postives = 513/743 (69.04%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD +  T+SHH +L ++LGSKE++  SMV+S++HGFSGFAAKLT+S A
Sbjct: 33  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQA 92

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +K+A++P V+ V     YK  TTR+WDYLGL  SP    NLL++  MG+ +IIGIIDSG 
Sbjct: 93  KKIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGV 152

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADY----GKEK 215
           WPES+ F D  +GP+PS WKG CE G+ FNS++CNKK+IGA++F   F+A +      E 
Sbjct: 153 WPESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSES 212

Query: 216 LVNEYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQWN--- 275
           L  +++S R  N HGTH A+ A GS+V   S+ G A GTVRGGAP AR+A+YK  W    
Sbjct: 213 L--DFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDL 272

Query: 276 --GVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVV 335
                +SADILKA+DEAIHDGVDVLS+S+ GF PLYPET+  + IA G+FHAV +GI VV
Sbjct: 273 DIAACSSADILKAMDEAIHDGVDVLSLSL-GFEPLYPETDVRDGIATGAFHAVLKGITVV 332

Query: 336 CAGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTEL 395
           CA GN GP  Q+VGNTAPWILTVAA+T+DR+ ++ ++LG+N    GQ+ +   +   T L
Sbjct: 333 CAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSL 392

Query: 396 I-----------SMGRCDKNLGNESSIIQFIRGKLVLCFLDVVDMSIIGLAGLAVVKQYG 455
           +             G C++ L N +   + + GK+VLCF +      +  A   V +  G
Sbjct: 393 VYPENPGNSNESFSGTCERLLINSN---RTMAGKVVLCFTESPYSISVTRAAHYVKRAGG 452

Query: 456 SGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPIS 515
            G+I+AGQ  N+L P C D F C+ VD ++GT + FY  +N  P+V+I+ ++T+IG P+ 
Sbjct: 453 LGVIIAGQPGNVLRP-CLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVG 512

Query: 516 TRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAG 575
           T++A FSSRGPN  S AILKPDIAAPG +ILAA   +  F+D GF   SGTSM+TP ++G
Sbjct: 513 TKVASFSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISG 572

Query: 576 IIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAA 635
           I+ALLK +HP WSPAAI+SA++TTA   DP G  IF++G+P K ADPFDYGGG+V+P  A
Sbjct: 573 IVALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKA 632

Query: 636 MDPGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTN 695
             PGL+Y+L   DY+ YMC +GY    IS L  + T C   KPS+L+ NLP+IT+P L  
Sbjct: 633 TKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKE 692

Query: 696 STTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNH 755
             T+ RT+TNVG L S+YR  VE P G +V+V P  L FNS  K++SFKV++STT   N 
Sbjct: 693 EVTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINT 752

Query: 756 GYSFGTLTWSDGVHLVKSPLSIK 759
           GY FG+LTWSD +H V  PLS++
Sbjct: 753 GYYFGSLTWSDSLHNVTIPLSVR 766

BLAST of Sed0012357 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 3.6e-212
Identity = 383/779 (49.17%), Postives = 526/779 (67.52%), Query Frame = 0

Query: 1   MVNKQASF--ILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHH 60
           M+N + S   +L+ + F  +   F  +  A+      KV+IVYLGE+Q+DD +  T+SHH
Sbjct: 1   MMNYRTSIYVVLSLVIFLNVQRSFVAESSAKR-----KVHIVYLGEKQHDDPEFVTESHH 60

Query: 61  DLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTT 120
            +L ++LGSKE + +SMVYSY+HGFSGFAAKLT+S A+K+A++P V+ V   S YK  TT
Sbjct: 61  RMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATT 120

Query: 121 RSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRC 180
           R+WDYLGLS++     +LLH   MG+ +IIG+ID+G WPES+ F D G GP+PS WKG C
Sbjct: 121 RTWDYLGLSAA--NPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGC 180

Query: 181 EVGQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVN--EYLSARDMNSHGTHTASTAAGS 240
           E G+ FNS+NCNKK+IGA++F  GF+A+       N  +++S RD++ HGTH ++ A GS
Sbjct: 181 ETGENFNSSNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGS 240

Query: 241 FVTKVSFYGQATGTVRGGAPLARLAIYKVQW------NGVGTSADILKAIDEAIHDGVDV 300
           FV  +S+ G A GTVRGGAP A +A+YK  W          +SADILKA+DEA+HDGVDV
Sbjct: 241 FVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDV 300

Query: 301 LSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVA 360
           LS+S+G  +PLY ET+  + I  G+FHAV +GI VVC+GGN GP   +V NTAPWI+TVA
Sbjct: 301 LSISLGSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVA 360

Query: 361 ASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELI-----------SMGRCDKNLGNE 420
           A+T+DR+  + ++LG+N    GQ+ +       T L+             G C++ L N 
Sbjct: 361 ATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNS 420

Query: 421 SSIIQFIRGKLVLCFLDVVDMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACI 480
           +  ++   GK+VLCF        +  A   V +  G G+I+A      ++P C D F C+
Sbjct: 421 NRTME---GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQP-CLDDFPCV 480

Query: 481 IVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIA 540
            VD ++GT +  YT ++  P+V+I+ +KT++G P+ T++A FSSRGPNS +PAILKPDIA
Sbjct: 481 AVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIA 540

Query: 541 APGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITT 600
           APG +ILAA   +  FSD GF M SGTSM+ P ++G+ ALLK +H  WSPAAI+SA++TT
Sbjct: 541 APGVSILAAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTT 600

Query: 601 ARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYIHYMCGMGYK 660
           A   DP G  IF++G+PPKLADPFDYGGG+V+P  + +PGL+Y++   DY+ YMC +GY 
Sbjct: 601 AWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYN 660

Query: 661 IPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEA 720
              IS L  + T C + KPS+L+ NLP+IT+P L +  T+TRTVTNVG L S+YR  VE 
Sbjct: 661 ETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEP 720

Query: 721 PQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIK 759
           P G +V+V P  L FNS  KK+ FKV +STT   N GY FG+LTWSD +H V  PLS++
Sbjct: 721 PLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVR 767

BLAST of Sed0012357 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 2.0e-210
Identity = 379/741 (51.15%), Postives = 510/741 (68.83%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD +  T+SHH +L ++LGSKE +  SMV+SY+HGFSGFAAKLTKS A
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +KLA++P V+ V   S Y+  TTR+WDYLGLS +     NLL+   MG+ VIIGI+DSG 
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA--NPKNLLNDTNMGEEVIIGIVDSGV 148

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADY----GKEK 215
           WPES+ F D G+GP+PS WKG C  G+ F S+ CNKK+IGA++F  GF+A +      E 
Sbjct: 149 WPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTES 208

Query: 216 LVNEYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQWN--- 275
           L  +++S RD + HGTH A+ A GS+V  +S+ G A GTVRGGAP AR+A+YK  W    
Sbjct: 209 L--DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDR 268

Query: 276 ---GVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHV 335
                 +SADILKA+DEA+HDGVDVLS+SIG   P +PET+    IA G+FHAV +GI V
Sbjct: 269 FDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITV 328

Query: 336 VCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTE 395
           VC+GGN GP  Q+VGNTAPWILTVAA+T+DR+  + I+LG+N    GQ+ +   +   T 
Sbjct: 329 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTS 388

Query: 396 LI---SMGRCDKNLGNESSIIQF-----IRGKLVLCFLDVVDMSIIGLAGLAVVKQYGSG 455
           L+   + G  +++   +  ++ F     + GK+VLCF        +  A   V +  G G
Sbjct: 389 LVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGGLG 448

Query: 456 IIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTR 515
           +IVA    + L P C+D F C+ VD ++GT +  Y  +   P+V+I+ +KT++G P+ T+
Sbjct: 449 VIVARNPGDNLSP-CEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTK 508

Query: 516 IAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGII 575
           +A FSSRGPNS  PAILKPDIAAPG +ILAA   +  F+D GF   SGTSM+ P ++G++
Sbjct: 509 VADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVV 568

Query: 576 ALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMD 635
           ALLK +H  WSPAAI+SA++TTA   DP G  IF++G+P KLADPFDYGGG+V+P  A  
Sbjct: 569 ALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAK 628

Query: 636 PGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNST 695
           PGL+Y+L   DY+ YMC +GY    IS L  + T C + KPS+L+ NLP+IT+P L +  
Sbjct: 629 PGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKDEV 688

Query: 696 TVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGY 755
           T+TRT+TNVG L S+Y+ V+E P GI+V+V P  L FNS  K++SFKV +STT   N GY
Sbjct: 689 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 748

Query: 756 SFGTLTWSDGVHLVKSPLSIK 759
            FG+LTWSD +H V  PLS++
Sbjct: 749 FFGSLTWSDSLHNVTIPLSVR 764

BLAST of Sed0012357 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 1.3e-209
Identity = 377/738 (51.08%), Postives = 506/738 (68.56%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD K  T+SHH +L+++LGSK+ + ESMVYSY+HGFSGFAAKLTKS A
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +K+A+ P VI V   S Y+  TTR WDYLG S+    S NL+    MGD  IIG+ID+G 
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLGPSAD--NSKNLVSDTNMGDQTIIGVIDTGV 147

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVN- 215
           WPES+SF D G+GP+PS WKG CE G+ F STNCN+K+IGA++F  GF+A+       + 
Sbjct: 148 WPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNATESP 207

Query: 216 EYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQW-----NG 275
           +Y+SARD + HGTH AS A GSFV  VS+ G   GT+RGGAP AR+A+YK  W     +G
Sbjct: 208 DYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINELDG 267

Query: 276 VGTS-ADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVCA 335
           V  S +DI+KAIDEAIHDGVDVLS+S+GG +PL  ET+  + IA G+FHAVA+GI VVCA
Sbjct: 268 VTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVVVCA 327

Query: 336 GGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELIS 395
           GGN GP  Q+V NTAPWILTVAA+T+DR+  + I LG+N    GQ+ +   +   T L+ 
Sbjct: 328 GGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGNNQVILGQAMYIGPELGFTSLVY 387

Query: 396 MGRCDKNLGNESSIIQFIR--------GKLVLCFLDVVDMSIIGLAGLAVVKQYGSGIIV 455
                 ++   S + + +         GK+VLCF    D +++  A   V    G G+I+
Sbjct: 388 PEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTVVSTAASIVKAAGGLGLII 447

Query: 456 AGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTRIAY 515
           A      L P C D F C+ +D ++GT + FY      P+V+I+ ++T++G P+ T++A 
Sbjct: 448 ARNPGYNLAP-CSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTKVAT 507

Query: 516 FSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGIIALL 575
           FSSRGPNS SPAILKPDIAAPG +ILAA  P+   +  GF M SGTSM+ P ++G+IALL
Sbjct: 508 FSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIALL 567

Query: 576 KCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMDPGL 635
           K +HP WSPAA +SA++TTA   DP G  I ++ +  K+ DPFDYGGG+V+P  A +PGL
Sbjct: 568 KSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEPGL 627

Query: 636 IYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNSTTVT 695
           I ++D+ DY+ Y+C  GY    IS L  + T C + KPS+L++NLP+IT+P L +  T+T
Sbjct: 628 ILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVCSNPKPSVLDINLPSITIPNLKDEVTLT 687

Query: 696 RTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGYSFG 755
           RTVTNVG + S+Y+ +VE P GI+V V P  L FNSK K +SF V +STT   N G+ FG
Sbjct: 688 RTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTGFYFG 747

Query: 756 TLTWSDGVHLVKSPLSIK 759
           +LTW+D +H V  P+S++
Sbjct: 748 SLTWTDSIHNVVIPVSVR 762

BLAST of Sed0012357 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 1.3e-209
Identity = 378/754 (50.13%), Postives = 516/754 (68.44%), Query Frame = 0

Query: 13  IPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSL 72
           I F ALF    L           KVY+VYLGE+++D+ +  T+SHH +L ++LGSKE  L
Sbjct: 5   ILFLALFLSIVLNVQISFVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVL 64

Query: 73  ESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYP 132
           +S+VYSY+HGFSGFAAKLT+S AQ+++E+P V++V  +++Y+  TTR+WDYLG+  SP  
Sbjct: 65  DSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGN 124

Query: 133 SSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQQFN-STNCNK 192
           S +LL +A MG NVI+G+IDSG WPES+ F D+G GPIPSRWKG CE G+ FN S +CN+
Sbjct: 125 SDSLLQKANMGYNVIVGVIDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNR 184

Query: 193 KVIGARWFSKGFIADYGKEKLVN--EYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATG 252
           K+IGA++F  G +A++G        EYLS RD   HGTH AST  GSF+  VS+ G   G
Sbjct: 185 KLIGAKYFVDGLVAEFGVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRG 244

Query: 253 TVRGGAPLARLAIYKVQWNGVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEEN 312
           T RGGAP   +A+YK  W+G  + AD+LKA+DEAIHDGVD+LS+S+G  +PL+PET    
Sbjct: 245 TARGGAPGVHIAVYKACWSGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEH-- 304

Query: 313 SIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTT 372
             ++G+FHAVA+GI VV A GN GP  Q++ N APW+LTVAA+T DR+  ++I+LG+N T
Sbjct: 305 -TSVGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNIT 364

Query: 373 YFGQSFFNSKK-----DLVTELISMGRCDKNLGNESSIIQFIRGKLVLCFLDVVDMSIIG 432
             GQ+ +   +         E    G C+K   N +S ++   GK+VLCF      +   
Sbjct: 365 ILGQAIYGGPELGFVGLTYPESPLSGDCEKLSANPNSTME---GKVVLCFAASTPSN--- 424

Query: 433 LAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIK 492
            A  AV+   G G+I+A    + L P     F  + +D ++GT + FY  +   P+V+I+
Sbjct: 425 AAIAAVINAGGLGLIMAKNPTHSLTP--TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQ 484

Query: 493 RAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMES 552
            +KT+ G  +ST++A FSSRGPNS SPAILKPDIAAPG NILAA+ P++  +D GF M S
Sbjct: 485 ASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMS 544

Query: 553 GTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFD 612
           GTSM+TP V+G++ LLK +HP WSP+AIKSA++TTA   DPSG PIF+DG+  KLADPFD
Sbjct: 545 GTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFD 604

Query: 613 YGGGVVDPNAAMDPGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLN 672
           YGGG+++P  A+ PGLIY++ T DY+ YMC + Y    IS +  + T CP+ KPS+L+LN
Sbjct: 605 YGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLN 664

Query: 673 LPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFK 732
           LP+IT+P L    T+TRTVTNVG + S+Y+ V++ P GI V+V P  L F+    K SF 
Sbjct: 665 LPSITIPNLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFT 724

Query: 733 VTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIK 759
           V +STT   N GY FG+LTW+D +H V  P+S++
Sbjct: 725 VRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVR 745

BLAST of Sed0012357 vs. ExPASy TrEMBL
Match: A0A6J1ETT0 (subtilisin-like protease SBT3.7 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 2.6e-282
Identity = 502/765 (65.62%), Postives = 612/765 (80.00%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+ +SF+   +  +A+FS      +A++D+QN KVYI YLGER Y+D KLTT SHH+LL 
Sbjct: 4   NRASSFMHAVVTIYAVFS-----AMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +V+GSKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRSWD
Sbjct: 64  SVMGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++G+
Sbjct: 124 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG +P
Sbjct: 244 YFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L+
Sbjct: 304 LPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLT 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D+ 
Sbjct: 364 SITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFIDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++ K
Sbjct: 424 MKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSDEK 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  D 
Sbjct: 484 ALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+PPK
Sbjct: 544 GFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FNS 
Sbjct: 664 ASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. ExPASy TrEMBL
Match: A0A6J1ETU3 (subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 5.9e-282
Identity = 503/765 (65.75%), Postives = 612/765 (80.00%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+ +SF+   +  +A+FS      +A++D+QN KV+IVYLGER YDD KLTT SHH+LL 
Sbjct: 4   NRASSFMHAVVTIYAVFS-----AMAEADNQNSKVHIVYLGERPYDDVKLTTHSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +VL SKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRSWD
Sbjct: 64  SVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++G+
Sbjct: 124 FLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG +P
Sbjct: 244 YFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L+
Sbjct: 304 LPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLT 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D+ 
Sbjct: 364 SITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFIDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++ K
Sbjct: 424 MKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSDEK 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  D 
Sbjct: 484 ALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+PPK
Sbjct: 544 GFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FNS 
Sbjct: 664 ASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.3e-281
Identity = 503/767 (65.58%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 1   MVNKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDL 60
           M N QA   +  +    ++++F+   +A+ DDQN KV+IVYLGER YDD KLTT SHH+L
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFS--AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSHHEL 60

Query: 61  LTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRS 120
           L +VL SKEKSLESMVYSYKHGFSGFAAKLT S AQKLA MP V +VF SS+YK  TTRS
Sbjct: 61  LESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRS 120

Query: 121 WDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEV 180
           WD+LGLSSSP  SSNLLHRAKMGDNVIIG+IDSGFWPES+SF D+G+GPIPSRWKG C++
Sbjct: 121 WDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQL 180

Query: 181 GQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTK 240
           G+ FNS +CNKKVIGARWF++  IAD+G+E +  +YLSARD   HGTHTASTA G+FV  
Sbjct: 181 GEDFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRN 240

Query: 241 VSFYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGF 300
           VS++G   GT+RGGAPLARLAIYKV W+   +G++ADILK IDEAIHDGVDVLSMSIGG 
Sbjct: 241 VSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGS 300

Query: 301 LPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRAL 360
           +PL P+ +E N++AIGSFHA+ARG+ VVCAGGNEG  QQ+V N APW+ TVAAST+DRA 
Sbjct: 301 IPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAF 360

Query: 361 LSSI-SLGDNTTYFGQSFFNSKKDLVTELISMG-RCDKNLGNESSIIQFIRGKLVLCFLD 420
           L+SI +LGDN TY GQ+F   KKD+V +L+ M  RC  +LG++   I      ++LCF+D
Sbjct: 361 LTSITTLGDNATYLGQTFL--KKDVVGKLVVMDRRCAGHLGSD---IPTGGNVVLLCFID 420

Query: 421 VVDMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLAN 480
           +   +      + V +    G+I AGQ  +IL P CD    CI VDT VGTKL  Y L++
Sbjct: 421 LAMKAAASNPVMPVKQAKVVGVIYAGQHTDILGP-CD--VPCIHVDTHVGTKLLTYILSD 480

Query: 481 SKPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFS 540
            K ++R+K  +TI+G PIS+RIAYFSSRGPNS SP ILKPDIAAPGSNI+AAVPP+ +  
Sbjct: 481 EKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGG 540

Query: 541 DNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNP 600
           D GF   SGTSM+TPH++GI+AL+K + PTWSPAAIKSALITTARV+DPSGLPIF++G+P
Sbjct: 541 DKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSP 600

Query: 601 PKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPS 660
           PK+ADPFDYGGGVVD NAA+DPGLIY+L   DYI +YMC MGY   +IS L+QQK  CPS
Sbjct: 601 PKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPS 660

Query: 661 LKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFN 720
            + S+L+LNLPTITVPALTNSTTVTRTVTNVGNLT++Y+AV++AP G KV V PRVL FN
Sbjct: 661 KRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFN 720

Query: 721 SKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           S +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 721 SNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Sed0012357 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 2.2e-281
Identity = 500/765 (65.36%), Postives = 605/765 (79.08%), Query Frame = 0

Query: 3   NKQASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDLLT 62
           N+  SFIL  +  +A+FS      +A++DDQN KV+IVYLGER YDD KLTT SHH+LL 
Sbjct: 4   NRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRSWD 122
           +VLGSKEKSLESMVYSY+HGFSGFAAKLT S AQKLA MP V +VF SS+YK +TTRSWD
Sbjct: 64  SVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWD 123

Query: 123 YLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEVGQ 182
           +LGLSSSP  SSNL HRAKMGDNVIIG+ID+GFWPES+SF D+G+GPIPSRWKG C+ G+
Sbjct: 124 FLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGE 183

Query: 183 QFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTKVS 242
            FNS++CNKKVIGARWF+   IAD+G+E +  +YLSARD   HGTHTASTA G+FV  VS
Sbjct: 184 DFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVS 243

Query: 243 FYGQATGTVRGGAPLARLAIYKVQWNG--VGTSADILKAIDEAIHDGVDVLSMSIGGFLP 302
           ++G   GT+RGGAPLARLAIYKV W+   +G+ ADILK IDEAIHDGVDVLSMSIG  +P
Sbjct: 244 YFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIP 303

Query: 303 LYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLS 362
           L+P+ N+ N +A+GSFHA+A+GI VVCAGGNEG  QQ+V N APW+ TVAAST+DRA L 
Sbjct: 304 LFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLV 363

Query: 363 SI-SLGDNTTYFGQSFFNSKKDLVTELISM-GRCDKNLGNESSIIQFIRGKLVLCFLDVV 422
           SI +LGDN TY GQ F   +KD+V  L++M GRC   LG +   I      ++LCF D+ 
Sbjct: 364 SITTLGDNATYLGQMFL--RKDIVGMLVAMDGRCAGILGGD---IPISGNVVLLCFTDLA 423

Query: 423 DMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSK 482
             +    A +   +    G+I AGQQ + L P CD    CI VDT VGTKL  Y L +  
Sbjct: 424 KTAPASNAVMPGKQAKVVGVIYAGQQTDNLVP-CD--IPCIHVDTHVGTKLFTYLLNDDD 483

Query: 483 PMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDN 542
            ++R++  +TIIG PIS+RIAYFSSRGPNS SP ILKPDIAAPG+NI+AAVPP+ +  D 
Sbjct: 484 ALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDK 543

Query: 543 GFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPK 602
           GF   SGTSM+TPH++GI+ L+K +HPTWSPAAIKSALITTARV+DPSG+PIF++G+PPK
Sbjct: 544 GFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPK 603

Query: 603 LADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTKCPSLK 662
           +ADPFDYGGGVVD NAA+DPGLIY+L   DYI +Y+C MGY   DIS L+QQKT CPS +
Sbjct: 604 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKR 663

Query: 663 PSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSK 722
            S+L+LNLPTITVPAL NSTTVTRTVTNVGNLT++Y+AV++AP G KV VKPRVL FNS 
Sbjct: 664 ASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSN 723

Query: 723 MKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
           +KKISFKV  S+T  +N+GYSFG+LTW+DGVHLVKSPLS++ DFF
Sbjct: 724 VKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Sed0012357 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 1.1e-280
Identity = 508/770 (65.97%), Postives = 601/770 (78.05%), Query Frame = 0

Query: 2   VNKQ-ASFILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHHDL 61
           +N Q A  I+ FI F+ L        LA+S+DQNPKV+IVYLGE+ + D+K TT SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTTRS 121
           L  +LGSKEKSLE+MVYSYKHGFSGFAAKLTKS AQKL+EM  V++V  SS+YK  TTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRCEV 181
           WD+LGLSSSP  SSNLLHRAK GD+VIIG+IDSG WPES+SF+D+GLGPIPSRWKG CE 
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVNEYLSARDMNSHGTHTASTAAGSFVTK 241
           G+QFNSTNCNKK+IGARWF K F+ADYG+E L  EYLS RD++ HGTHTASTAAGSFV  
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 VSFYGQATGTVRGGAPLARLAIYKVQW--NGVGTSADILKAIDEAIHDGVDVLSMSIGGF 301
           ++++  A GT RGGAPLARLAIYK  W   GVG+SADILKAIDEAIHDGVDVLS+SIGG 
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 LPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVAASTMDRAL 361
            P YPE  E + IA GSFHA+ +GI VVCA GN GP  Q V N APWI TVA +T+DRA 
Sbjct: 303 PPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAF 362

Query: 362 LSSI-SLGDNTTYFGQSFFNSKKDLVTELISMGRCDKNLGNESSIIQFIRGKLVLCFLDV 421
           LSSI +L DNTT+ GQS   SKKDLV EL+S  RCD+  GNE+    FI GK+VLCF  +
Sbjct: 363 LSSITTLRDNTTFMGQSLLESKKDLVAELVSWERCDQLSGNEA----FINGKVVLCFPKL 422

Query: 422 VDMSIIGLAGLAVVKQYGSGIIVAGQQ--NNILEPRCDDS-FACIIVDTDVGTKLHFYTL 481
            D S I  A   VV+  G+GIIVAGQQ  NN+L   C  S   CI+VDT VG+KL FY L
Sbjct: 423 ADYSTISKAAEVVVRANGTGIIVAGQQVDNNLL--ACISSPIPCILVDTIVGSKLFFYFL 482

Query: 482 ANS-KPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHA 541
            NS  P+V ++ A+TIIG PI+  IA+FSSRGPNS SP ILKPDI+APGSNIL+AV PH 
Sbjct: 483 QNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHY 542

Query: 542 EFSDNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSD 601
            F++ GF + SGTSM+TPHV+ I+ALLK VHPTWSPAAIKSAL+TTAR +   GLPIF+D
Sbjct: 543 FFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAD 602

Query: 602 GNPPKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYI-HYMCGMGYKIPDISLLTQQKTK 661
           G PPK+ADPFDYG GVVD NAA+DPGLIY++   DYI +Y+CGMGY+  DIS LT +KT+
Sbjct: 603 GTPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTE 662

Query: 662 CPSLKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVL 721
           CP  + S+L+LNLP IT+P+L NST VTRTVTNVGNL+ +Y+A +EAP G KVSV P+VL
Sbjct: 663 CPLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVL 722

Query: 722 AFNSKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIKVDFF 763
            FNS++KKISFKV   T   +N+GYSFG LTWSDGVH+VK PLS++  FF
Sbjct: 723 VFNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGFF 761

BLAST of Sed0012357 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.5e-213
Identity = 384/742 (51.75%), Postives = 512/742 (69.00%), Query Frame = 0

Query: 37  VYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQ 96
           V+IVYLGE+Q+DD +  T+SHH +L ++LGSKE++  SMV+S++HGFSGFAAKLT+S A+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 97  KLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFW 156
           K+A++P V+ V     YK  TTR+WDYLGL  SP    NLL++  MG+ +IIGIIDSG W
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVW 141

Query: 157 PESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADY----GKEKL 216
           PES+ F D  +GP+PS WKG CE G+ FNS++CNKK+IGA++F   F+A +      E L
Sbjct: 142 PESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL 201

Query: 217 VNEYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQWN---- 276
             +++S R  N HGTH A+ A GS+V   S+ G A GTVRGGAP AR+A+YK  W     
Sbjct: 202 --DFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLD 261

Query: 277 -GVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVC 336
               +SADILKA+DEAIHDGVDVLS+S+ GF PLYPET+  + IA G+FHAV +GI VVC
Sbjct: 262 IAACSSADILKAMDEAIHDGVDVLSLSL-GFEPLYPETDVRDGIATGAFHAVLKGITVVC 321

Query: 337 AGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELI 396
           A GN GP  Q+VGNTAPWILTVAA+T+DR+ ++ ++LG+N    GQ+ +   +   T L+
Sbjct: 322 AAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLV 381

Query: 397 -----------SMGRCDKNLGNESSIIQFIRGKLVLCFLDVVDMSIIGLAGLAVVKQYGS 456
                        G C++ L N +   + + GK+VLCF +      +  A   V +  G 
Sbjct: 382 YPENPGNSNESFSGTCERLLINSN---RTMAGKVVLCFTESPYSISVTRAAHYVKRAGGL 441

Query: 457 GIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPIST 516
           G+I+AGQ  N+L P C D F C+ VD ++GT + FY  +N  P+V+I+ ++T+IG P+ T
Sbjct: 442 GVIIAGQPGNVLRP-CLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGT 501

Query: 517 RIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGI 576
           ++A FSSRGPN  S AILKPDIAAPG +ILAA   +  F+D GF   SGTSM+TP ++GI
Sbjct: 502 KVASFSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGI 561

Query: 577 IALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAM 636
           +ALLK +HP WSPAAI+SA++TTA   DP G  IF++G+P K ADPFDYGGG+V+P  A 
Sbjct: 562 VALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKAT 621

Query: 637 DPGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNS 696
            PGL+Y+L   DY+ YMC +GY    IS L  + T C   KPS+L+ NLP+IT+P L   
Sbjct: 622 KPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEE 681

Query: 697 TTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHG 756
            T+ RT+TNVG L S+YR  VE P G +V+V P  L FNS  K++SFKV++STT   N G
Sbjct: 682 VTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTG 741

Query: 757 YSFGTLTWSDGVHLVKSPLSIK 759
           Y FG+LTWSD +H V  PLS++
Sbjct: 742 YYFGSLTWSDSLHNVTIPLSVR 754

BLAST of Sed0012357 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.5e-213
Identity = 376/742 (50.67%), Postives = 511/742 (68.87%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD +  T+SHH +L ++LGSKE + +SMVYSY+HGFSGFAAKLT+S A
Sbjct: 49  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQA 108

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +K+A++P V+ V   S YK  TTR+WDYLGLS++     +LLH   MG+ +IIG+ID+G 
Sbjct: 109 KKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAA--NPKSLLHETNMGEQIIIGVIDTGV 168

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVN- 215
           WPES+ F D G GP+PS WKG CE G+ FNS+NCNKK+IGA++F  GF+A+       N 
Sbjct: 169 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNSTNS 228

Query: 216 -EYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQW------ 275
            +++S RD++ HGTH ++ A GSFV  +S+ G A GTVRGGAP A +A+YK  W      
Sbjct: 229 LDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDDDD 288

Query: 276 NGVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVC 335
               +SADILKA+DEA+HDGVDVLS+S+G  +PLY ET+  + I  G+FHAV +GI VVC
Sbjct: 289 TTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITVVC 348

Query: 336 AGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELI 395
           +GGN GP   +V NTAPWI+TVAA+T+DR+  + ++LG+N    GQ+ +       T L+
Sbjct: 349 SGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTSLV 408

Query: 396 -----------SMGRCDKNLGNESSIIQFIRGKLVLCFLDVVDMSIIGLAGLAVVKQYGS 455
                        G C++ L N +  ++   GK+VLCF        +  A   V +  G 
Sbjct: 409 YPENPGNSNESFSGTCEELLFNSNRTME---GKVVLCFTTSPYGGAVLSAARYVKRAGGL 468

Query: 456 GIIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPIST 515
           G+I+A      ++P C D F C+ VD ++GT +  YT ++  P+V+I+ +KT++G P+ T
Sbjct: 469 GVIIARHPGYAIQP-CLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGT 528

Query: 516 RIAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGI 575
           ++A FSSRGPNS +PAILKPDIAAPG +ILAA   +  FSD GF M SGTSM+ P ++G+
Sbjct: 529 KVATFSSRGPNSIAPAILKPDIAAPGVSILAAT-TNTTFSDQGFIMLSGTSMAAPAISGV 588

Query: 576 IALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAM 635
            ALLK +H  WSPAAI+SA++TTA   DP G  IF++G+PPKLADPFDYGGG+V+P  + 
Sbjct: 589 AALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSA 648

Query: 636 DPGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNS 695
           +PGL+Y++   DY+ YMC +GY    IS L  + T C + KPS+L+ NLP+IT+P L + 
Sbjct: 649 NPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDE 708

Query: 696 TTVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHG 755
            T+TRTVTNVG L S+YR  VE P G +V+V P  L FNS  KK+ FKV +STT   N G
Sbjct: 709 VTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 768

Query: 756 YSFGTLTWSDGVHLVKSPLSIK 759
           Y FG+LTWSD +H V  PLS++
Sbjct: 769 YYFGSLTWSDSLHNVTIPLSVR 783

BLAST of Sed0012357 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 739.6 bits (1908), Expect = 2.6e-213
Identity = 383/779 (49.17%), Postives = 526/779 (67.52%), Query Frame = 0

Query: 1   MVNKQASF--ILTFIPFFALFSMFALKPLAQSDDQNPKVYIVYLGERQYDDSKLTTQSHH 60
           M+N + S   +L+ + F  +   F  +  A+      KV+IVYLGE+Q+DD +  T+SHH
Sbjct: 1   MMNYRTSIYVVLSLVIFLNVQRSFVAESSAKR-----KVHIVYLGEKQHDDPEFVTESHH 60

Query: 61  DLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHAQKLAEMPGVIKVFRSSIYKTQTT 120
            +L ++LGSKE + +SMVYSY+HGFSGFAAKLT+S A+K+A++P V+ V   S YK  TT
Sbjct: 61  RMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATT 120

Query: 121 RSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGFWPESKSFRDEGLGPIPSRWKGRC 180
           R+WDYLGLS++     +LLH   MG+ +IIG+ID+G WPES+ F D G GP+PS WKG C
Sbjct: 121 RTWDYLGLSAA--NPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGC 180

Query: 181 EVGQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVN--EYLSARDMNSHGTHTASTAAGS 240
           E G+ FNS+NCNKK+IGA++F  GF+A+       N  +++S RD++ HGTH ++ A GS
Sbjct: 181 ETGENFNSSNCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGS 240

Query: 241 FVTKVSFYGQATGTVRGGAPLARLAIYKVQW------NGVGTSADILKAIDEAIHDGVDV 300
           FV  +S+ G A GTVRGGAP A +A+YK  W          +SADILKA+DEA+HDGVDV
Sbjct: 241 FVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDV 300

Query: 301 LSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVCAGGNEGPYQQSVGNTAPWILTVA 360
           LS+S+G  +PLY ET+  + I  G+FHAV +GI VVC+GGN GP   +V NTAPWI+TVA
Sbjct: 301 LSISLGSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVA 360

Query: 361 ASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELI-----------SMGRCDKNLGNE 420
           A+T+DR+  + ++LG+N    GQ+ +       T L+             G C++ L N 
Sbjct: 361 ATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNS 420

Query: 421 SSIIQFIRGKLVLCFLDVVDMSIIGLAGLAVVKQYGSGIIVAGQQNNILEPRCDDSFACI 480
           +  ++   GK+VLCF        +  A   V +  G G+I+A      ++P C D F C+
Sbjct: 421 NRTME---GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQP-CLDDFPCV 480

Query: 481 IVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTRIAYFSSRGPNSASPAILKPDIA 540
            VD ++GT +  YT ++  P+V+I+ +KT++G P+ T++A FSSRGPNS +PAILKPDIA
Sbjct: 481 AVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIA 540

Query: 541 APGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGIIALLKCVHPTWSPAAIKSALITT 600
           APG +ILAA   +  FSD GF M SGTSM+ P ++G+ ALLK +H  WSPAAI+SA++TT
Sbjct: 541 APGVSILAAT-TNTTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTT 600

Query: 601 ARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMDPGLIYELDTIDYIHYMCGMGYK 660
           A   DP G  IF++G+PPKLADPFDYGGG+V+P  + +PGL+Y++   DY+ YMC +GY 
Sbjct: 601 AWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYN 660

Query: 661 IPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNSTTVTRTVTNVGNLTSIYRAVVEA 720
              IS L  + T C + KPS+L+ NLP+IT+P L +  T+TRTVTNVG L S+YR  VE 
Sbjct: 661 ETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEP 720

Query: 721 PQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGYSFGTLTWSDGVHLVKSPLSIK 759
           P G +V+V P  L FNS  KK+ FKV +STT   N GY FG+LTWSD +H V  PLS++
Sbjct: 721 PLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVR 767

BLAST of Sed0012357 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 733.8 bits (1893), Expect = 1.4e-211
Identity = 379/741 (51.15%), Postives = 510/741 (68.83%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD +  T+SHH +L ++LGSKE +  SMV+SY+HGFSGFAAKLTKS A
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +KLA++P V+ V   S Y+  TTR+WDYLGLS +     NLL+   MG+ VIIGI+DSG 
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA--NPKNLLNDTNMGEEVIIGIVDSGV 148

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADY----GKEK 215
           WPES+ F D G+GP+PS WKG C  G+ F S+ CNKK+IGA++F  GF+A +      E 
Sbjct: 149 WPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTES 208

Query: 216 LVNEYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQWN--- 275
           L  +++S RD + HGTH A+ A GS+V  +S+ G A GTVRGGAP AR+A+YK  W    
Sbjct: 209 L--DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDR 268

Query: 276 ---GVGTSADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHV 335
                 +SADILKA+DEA+HDGVDVLS+SIG   P +PET+    IA G+FHAV +GI V
Sbjct: 269 FDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITV 328

Query: 336 VCAGGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTE 395
           VC+GGN GP  Q+VGNTAPWILTVAA+T+DR+  + I+LG+N    GQ+ +   +   T 
Sbjct: 329 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTS 388

Query: 396 LI---SMGRCDKNLGNESSIIQF-----IRGKLVLCFLDVVDMSIIGLAGLAVVKQYGSG 455
           L+   + G  +++   +  ++ F     + GK+VLCF        +  A   V +  G G
Sbjct: 389 LVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGGLG 448

Query: 456 IIVAGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTR 515
           +IVA    + L P C+D F C+ VD ++GT +  Y  +   P+V+I+ +KT++G P+ T+
Sbjct: 449 VIVARNPGDNLSP-CEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTK 508

Query: 516 IAYFSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGII 575
           +A FSSRGPNS  PAILKPDIAAPG +ILAA   +  F+D GF   SGTSM+ P ++G++
Sbjct: 509 VADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVV 568

Query: 576 ALLKCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMD 635
           ALLK +H  WSPAAI+SA++TTA   DP G  IF++G+P KLADPFDYGGG+V+P  A  
Sbjct: 569 ALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAK 628

Query: 636 PGLIYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNST 695
           PGL+Y+L   DY+ YMC +GY    IS L  + T C + KPS+L+ NLP+IT+P L +  
Sbjct: 629 PGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKDEV 688

Query: 696 TVTRTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGY 755
           T+TRT+TNVG L S+Y+ V+E P GI+V+V P  L FNS  K++SFKV +STT   N GY
Sbjct: 689 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 748

Query: 756 SFGTLTWSDGVHLVKSPLSIK 759
            FG+LTWSD +H V  PLS++
Sbjct: 749 FFGSLTWSDSLHNVTIPLSVR 764

BLAST of Sed0012357 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 731.1 bits (1886), Expect = 9.1e-211
Identity = 377/738 (51.08%), Postives = 506/738 (68.56%), Query Frame = 0

Query: 36  KVYIVYLGERQYDDSKLTTQSHHDLLTTVLGSKEKSLESMVYSYKHGFSGFAAKLTKSHA 95
           KV+IVYLGE+Q+DD K  T+SHH +L+++LGSK+ + ESMVYSY+HGFSGFAAKLTKS A
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 96  QKLAEMPGVIKVFRSSIYKTQTTRSWDYLGLSSSPYPSSNLLHRAKMGDNVIIGIIDSGF 155
           +K+A+ P VI V   S Y+  TTR WDYLG S+    S NL+    MGD  IIG+ID+G 
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLGPSAD--NSKNLVSDTNMGDQTIIGVIDTGV 147

Query: 156 WPESKSFRDEGLGPIPSRWKGRCEVGQQFNSTNCNKKVIGARWFSKGFIADYGKEKLVN- 215
           WPES+SF D G+GP+PS WKG CE G+ F STNCN+K+IGA++F  GF+A+       + 
Sbjct: 148 WPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNATESP 207

Query: 216 EYLSARDMNSHGTHTASTAAGSFVTKVSFYGQATGTVRGGAPLARLAIYKVQW-----NG 275
           +Y+SARD + HGTH AS A GSFV  VS+ G   GT+RGGAP AR+A+YK  W     +G
Sbjct: 208 DYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINELDG 267

Query: 276 VGTS-ADILKAIDEAIHDGVDVLSMSIGGFLPLYPETNEENSIAIGSFHAVARGIHVVCA 335
           V  S +DI+KAIDEAIHDGVDVLS+S+GG +PL  ET+  + IA G+FHAVA+GI VVCA
Sbjct: 268 VTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVVVCA 327

Query: 336 GGNEGPYQQSVGNTAPWILTVAASTMDRALLSSISLGDNTTYFGQSFFNSKKDLVTELIS 395
           GGN GP  Q+V NTAPWILTVAA+T+DR+  + I LG+N    GQ+ +   +   T L+ 
Sbjct: 328 GGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGNNQVILGQAMYIGPELGFTSLVY 387

Query: 396 MGRCDKNLGNESSIIQFIR--------GKLVLCFLDVVDMSIIGLAGLAVVKQYGSGIIV 455
                 ++   S + + +         GK+VLCF    D +++  A   V    G G+I+
Sbjct: 388 PEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTVVSTAASIVKAAGGLGLII 447

Query: 456 AGQQNNILEPRCDDSFACIIVDTDVGTKLHFYTLANSKPMVRIKRAKTIIGNPISTRIAY 515
           A      L P C D F C+ +D ++GT + FY      P+V+I+ ++T++G P+ T++A 
Sbjct: 448 ARNPGYNLAP-CSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTKVAT 507

Query: 516 FSSRGPNSASPAILKPDIAAPGSNILAAVPPHAEFSDNGFKMESGTSMSTPHVAGIIALL 575
           FSSRGPNS SPAILKPDIAAPG +ILAA  P+   +  GF M SGTSM+ P ++G+IALL
Sbjct: 508 FSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIALL 567

Query: 576 KCVHPTWSPAAIKSALITTARVKDPSGLPIFSDGNPPKLADPFDYGGGVVDPNAAMDPGL 635
           K +HP WSPAA +SA++TTA   DP G  I ++ +  K+ DPFDYGGG+V+P  A +PGL
Sbjct: 568 KSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEPGL 627

Query: 636 IYELDTIDYIHYMCGMGYKIPDISLLTQQKTKCPSLKPSMLNLNLPTITVPALTNSTTVT 695
           I ++D+ DY+ Y+C  GY    IS L  + T C + KPS+L++NLP+IT+P L +  T+T
Sbjct: 628 ILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVCSNPKPSVLDINLPSITIPNLKDEVTLT 687

Query: 696 RTVTNVGNLTSIYRAVVEAPQGIKVSVKPRVLAFNSKMKKISFKVTISTTSHKNHGYSFG 755
           RTVTNVG + S+Y+ +VE P GI+V V P  L FNSK K +SF V +STT   N G+ FG
Sbjct: 688 RTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTGFYFG 747

Query: 756 TLTWSDGVHLVKSPLSIK 759
           +LTW+D +H V  P+S++
Sbjct: 748 SLTWTDSIHNVVIPVSVR 762