Sed0012033 (gene) Chayote v1

Overview


Name	Sed0012033
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Phosphomethylpyrimidine synthase
Location	LG13: 19789292 .. 19793341 (+)
RNA-Seq Expression	Sed0012033
Synteny	Sed0012033

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCGTTCATGCTACCATAACACCGGCTGTTGGTAAGAGTGGGAACCGTTCCTATGCAACAAAATCACTTAGTACTGTCTTTTTGCCTGGGTTTGACGTGGTTGGCCGTGTTTCTGGTGCAAGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCTACTTTAACCTTTGAACCCTCAGAAACCAGTACAGAGAAGGCCAAAAACAAGAAACATACTGTTGATCCTTCATCTCCTGATTTTCTTCCGCTTCCTTCATTTGAACAATGTTTCCCAAGAAGCACAAAAGAACACAAGTGAGTGAATTCATTGGATTATCATATATCATAAATGAAATCATATACATTGCAGCTTTTAATTTAACCACATTTTAGGTGTGGATTTGAATTCACGACATCTTGGTCACTAAGTCATATATCATGCTACAGAACTACGCTCTTGTTTGCTATGCATTTGCAATTGTTGATTTTTTCATTTTTATTTTTTGCTTTACAGGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGGCGTGTTCATTTATCTGGCGATGAGCCAAACTTTGACAACTATGATACTAGTGGTCCTCAAAACATCAGCCCTCGCATTGGTAAGATGATACACTATTAACTGTTTCTTGATAAAGACTCATTTTTGCTATATTTGTATTGCAGCATTTATTGGTTTTGTTTGTTGTTTTTTCAGTGTCTTGTGAATGTATATTTTTACTCGAAATATAATTTTTATGAAAACTATTTGGAGTCTTTGTTGCTCATATACATATACATTTGTTATTTTAAGTCTTCTGCATAAGAGGGATATTTTTTCGTAAACATTCAAACCAACTAGTGCCGAGCTGAAAAAACTTTACCATTTTGAAGTGAGCTTATCTGACTCTATAGGGAAAAAAGGCGGAAAGTATGATTGCTTTCTATTATGCAAAGTCAATTTGGAATTTCGTTAAATTTTCATAATACAATTTCTAGACATGATTCTTCCTGATTCAACTTCCATGACGCATCAATGCAAATCAGTAATGGTTGTTTTTTTCTTCTTTTGTATCCTATGCTGTATACTGGTTACTTCTGGCTTTCAATTTATATTGTTACTAACTATCAATAATGTCATATAGGATTGCCTAAGCTTCGAAAGGACTGGGTTGATAGGAGGGAAAAATTAGGAACACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGCTGTTTTGTGCCAATCGTGAGAAGCTCGAACCAGAGTTTGTGAGGTCAGAGGTCGCTCGTGGGCGAGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCGATTGAAGAAGAAGTTTATAAAGTTCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTTTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATTTTGCGTAACTCAGCAGTACCAGTTGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTAAATGGCATAGCTGAAAATCTCACGTGGGAGATATTAAGGGAAACGCTGATTGAACAAGCAGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTACGATACATCCCTCTAACAGCAAAGCGAATGACGGGAATTGTATCACGTGGAGGATCTATCCATGCAAAGTGGTGTTTAGCTCATCATAAGGAAAACTTTGCTTATGAACACTGGGACGATATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTCGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAGTGAATAAAAAGTTTTTCTTGCTTACATACATGCACATGCTCAAAATCAGACTTATGCTATAATCATTTGATAGCAAACCTAAGTTATATGAATTTGAAAGAATATGTCATAGTTGTAACTTTTGTAAGAACCACTCAAGCCTAGTATATGAATTTGAAAGGTCATAGTTGCAACTTTTGTAAGAACCACTCAAGCCTTACCTATTAGAGTGTTTTACAAGGCTCACTCAGGTGCTCGGCTCAAGGCACAGGTCTAGCACATCGCCATGCGCCTCGTAATAAAGCATGCCCTCCTTATGATTAATTATAGAAAAATAAGTTTTACTAAACCTAAATGCCTAAGCTTCAGTAGACTATTATTGGGACCTAAGTGTGGGTTGAGCTTTTAAAAACACTGCCTATTAAAAGCTTAAGTGCTAGAAATTTTAGGACCCTCTCAGAGCAGTATCTTAAGATTTCATCACCCTTTAAATTTCACCATGCGAGTTTGGTTGAGAGCTCCAATTTATAATATAGGGCACTTCTGTTTAGTAGTGGATTCTTTTTATTTCTTTTGCACAATTAGTTTAATGGTAATGTACATTAAAAGTATAGAACAAAGATTTCCATTATTTCAATCACTAATAAATGCTATTTTTTGGTTTAGGAACTATGTATGTTATTTTTTTCGCATTATTAACTAGTGGCCAAATATTGAGTTTTCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAATGGTGTAATGAAGCACCCTTTTACACTCTTGGCCCTTTGACTACGGATGTTGCTCCTGGATATGACCATATTACATCTGCCATTGGTGCTGCCAATATTGGAGCTCTTGGCACGGCACTTCTCTGTTACGTTACACCGAAAGAGCACCTTGGATTGCCAAATCGTGATGATGTGAAAGCTGGAGTAATAGCATACAAGATAGCTGCTCATGCAGCTGATCTAGCCAAATGTCACCTGCATGCGCAATCATGGGACGATGCGTTGAGCAAAGCGAGGTTTGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAGGATGTGCGAAAGTATGCCGAGGAACATGGGTATGGGAATGTAGAGGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGGGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCCTTGAAGAGATGAAGGTTAGTAATTATCTAAACTTTGGGCCTTCTGATTTTAATTCCTGGTTTTTTAAGCTAGAACAAGAATTTGGATAACCATATATGCTGAATCAACTGAGACTTCTCAGTTGAGCATCTTTGCATCATATATGACAAATCACATTGGCGACCTGCTTGACATAATAATCAATTATTATTTTATTAGATTAAACCATCATGGATTGGTGTAGTAGTCAAGAAACTTTTTGAAAACAAAGAGAATTGATCAATCTATGGTAATCAACCTACCTAGAAATTAATTTCATATTAAGTTTCTTGATGTCTAAATGTTATTGGGTCAAACAGTATGTCTTGTGAAAATAGTTAACATACGCATAAGGTAAATATATATATAAAAAAAAGCGTTTGTCTATATTAATCATCTTTGTCTGTCAGAGGCGGGCCAATGATTTATATTTTTTTGTGTCTTGTGTATATTGTCAAATGGTAATAATTAGTTAGCCACAATATCGGGATTTTATGACTTCATTTTGAAAAGGGGAATGCAATCAATATCAGAATCGTTCCCTTTACGATAAGTTCGACTGAGAGCAAGGGGAACCATTGCTCTCGGCTTATAGCAATTACCCACTAATTCCTTGGTTAGCTCTTACAGTTACAATATATCAATTGCAGGATCACTAGGGATGGGGAAAA

mRNA sequence

ATGGCGTCCGTTCATGCTACCATAACACCGGCTGTTGGTAAGAGTGGGAACCGTTCCTATGCAACAAAATCACTTAGTACTGTCTTTTTGCCTGGGTTTGACGTGGTTGGCCGTGTTTCTGGTGCAAGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCTACTTTAACCTTTGAACCCTCAGAAACCAGTACAGAGAAGGCCAAAAACAAGAAACATACTGTTGATCCTTCATCTCCTGATTTTCTTCCGCTTCCTTCATTTGAACAATGTTTCCCAAGAAGCACAAAAGAACACAAGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGGCGTGTTCATTTATCTGGCGATGAGCCAAACTTTGACAACTATGATACTAGTGGTCCTCAAAACATCAGCCCTCGCATTGGATTGCCTAAGCTTCGAAAGGACTGGGTTGATAGGAGGGAAAAATTAGGAACACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGCTGTTTTGTGCCAATCGTGAGAAGCTCGAACCAGAGTTTGTGAGGTCAGAGGTCGCTCGTGGGCGAGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCGATTGAAGAAGAAGTTTATAAAGTTCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTTTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATTTTGCGTAACTCAGCAGTACCAGTTGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTAAATGGCATAGCTGAAAATCTCACGTGGGAGATATTAAGGGAAACGCTGATTGAACAAGCAGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTACGATACATCCCTCTAACAGCAAAGCGAATGACGGGAATTGTATCACGTGGAGGATCTATCCATGCAAAGTGGTGTTTAGCTCATCATAAGGAAAACTTTGCTTATGAACACTGGGACGATATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTCGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAATGGTGTAATGAAGCACCCTTTTACACTCTTGGCCCTTTGACTACGGATGTTGCTCCTGGATATGACCATATTACATCTGCCATTGGTGCTGCCAATATTGGAGCTCTTGGCACGGCACTTCTCTGTTACGTTACACCGAAAGAGCACCTTGGATTGCCAAATCGTGATGATGTGAAAGCTGGAGTAATAGCATACAAGATAGCTGCTCATGCAGCTGATCTAGCCAAATGTCACCTGCATGCGCAATCATGGGACGATGCGTTGAGCAAAGCGAGGTTTGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAGGATGTGCGAAAGTATGCCGAGGAACATGGGTATGGGAATGTAGAGGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGGGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCCTTGAAGAGATGAAGGTTAGTAATTATCTAAACTTTGGGCCTTCTGATTTTAATTCCTGGTTTTTTAAGCTAGAACAAGAATTTGGATAACCATATATGCTGAATCAACTGAGACTTCTCAGTTGAGCATCTTTGCATCATATATGACAAATCACATTGGCGACCTGCTTGACATAATAATCAATTATTATTTTATTAGATTAAACCATCATGGATTGGTGTAGTAGTCAAGAAACTTTTTGAAAACAAAGAGAATTGATCAATCTATGGTAATCAACCTACCTAGAAATTAATTTCATATTAAGTTTCTTGATGTCTAAATGTTATTGGGTCAAACAGTATGTCTTGTGAAAATAGTTAACATACGCATAAGGTAAATATATATATAAAAAAAAGCGTTTGTCTATATTAATCATCTTTGTCTGTCAGAGGCGGGCCAATGATTTATATTTTTTTGTGTCTTGTGTATATTGTCAAATGGTAATAATTAGTTAGCCACAATATCGGGATTTTATGACTTCATTTTGAAAAGGGGAATGCAATCAATATCAGAATCGTTCCCTTTACGATAAGTTCGACTGAGAGCAAGGGGAACCATTGCTCTCGGCTTATAGCAATTACCCACTAATTCCTTGGTTAGCTCTTACAGTTACAATATATCAATTGCAGGATCACTAGGGATGGGGAAAA

Coding sequence (CDS)

ATGGCGTCCGTTCATGCTACCATAACACCGGCTGTTGGTAAGAGTGGGAACCGTTCCTATGCAACAAAATCACTTAGTACTGTCTTTTTGCCTGGGTTTGACGTGGTTGGCCGTGTTTCTGGTGCAAGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCTACTTTAACCTTTGAACCCTCAGAAACCAGTACAGAGAAGGCCAAAAACAAGAAACATACTGTTGATCCTTCATCTCCTGATTTTCTTCCGCTTCCTTCATTTGAACAATGTTTCCCAAGAAGCACAAAAGAACACAAGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGGCGTGTTCATTTATCTGGCGATGAGCCAAACTTTGACAACTATGATACTAGTGGTCCTCAAAACATCAGCCCTCGCATTGGATTGCCTAAGCTTCGAAAGGACTGGGTTGATAGGAGGGAAAAATTAGGAACACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGCTGTTTTGTGCCAATCGTGAGAAGCTCGAACCAGAGTTTGTGAGGTCAGAGGTCGCTCGTGGGCGAGCAATCATCCCTTCCAACAAGAAGCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCAAGTTCGATTGAAGAAGAAGTTTATAAAGTTCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTTTCTACTGGTCGCCACATACACGAGACTCGTGAGTGGATTTTGCGTAACTCAGCAGTACCAGTTGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTAAATGGCATAGCTGAAAATCTCACGTGGGAGATATTAAGGGAAACGCTGATTGAACAAGCAGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTACGATACATCCCTCTAACAGCAAAGCGAATGACGGGAATTGTATCACGTGGAGGATCTATCCATGCAAAGTGGTGTTTAGCTCATCATAAGGAAAACTTTGCTTATGAACACTGGGACGATATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAATGACACTGCTCAATTCGCAGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAATGGTGTAATGAAGCACCCTTTTACACTCTTGGCCCTTTGACTACGGATGTTGCTCCTGGATATGACCATATTACATCTGCCATTGGTGCTGCCAATATTGGAGCTCTTGGCACGGCACTTCTCTGTTACGTTACACCGAAAGAGCACCTTGGATTGCCAAATCGTGATGATGTGAAAGCTGGAGTAATAGCATACAAGATAGCTGCTCATGCAGCTGATCTAGCCAAATGTCACCTGCATGCGCAATCATGGGACGATGCGTTGAGCAAAGCGAGGTTTGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCCCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAGGATGTGCGAAAGTATGCCGAGGAACATGGGTATGGGAATGTAGAGGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAGTTTTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGGGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACTTGGACTCCTTGAAGAGATGA

Protein sequence

MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLTFEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRRVHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEEMLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAENLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNVEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR

Homology

[Close]

BLAST of Sed0012033 vs. NCBI nr
Match: XP_038894338.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida] >XP_038894339.1 phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1286.2 bits (3327), Expect = 0.0e+00
Identity = 622/647 (96.14%), Postives = 633/647 (97.84%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           MASVHATITPAVGKSGNRS+ATK L+T FLPGFDVVGRVSGA KDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSGNRSHATKPLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P  TSTEKAK+KKHTVDPSSPDFLPLPSFEQCFP+STKEHKE+VH+ETGHVLKVPFRR
Sbjct: 61  FDPPGTSTEKAKSKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAK GIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKHGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNK HLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEI RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICN YDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNLYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASY+DS KR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 647

[Close]

BLAST of Sed0012033 vs. NCBI nr
Match: XP_038894337.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1286.2 bits (3327), Expect = 0.0e+00
Identity = 622/647 (96.14%), Postives = 633/647 (97.84%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           MASVHATITPAVGKSGNRS+ATK L+T FLPGFDVVGRVSGA KDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSGNRSHATKPLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P  TSTEKAK+KKHTVDPSSPDFLPLPSFEQCFP+STKEHKE+VH+ETGHVLKVPFRR
Sbjct: 61  FDPPGTSTEKAKSKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAK GIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKHGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNK HLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEI RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICN YDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNLYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASY+DS KR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 647

[Close]

BLAST of Sed0012033 vs. NCBI nr
Match: XP_023519726.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 618/647 (95.52%), Postives = 629/647 (97.22%), Query Frame = 0


Query: 1    MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
            MASVHATITPAVGKSGN SY TKSL+T FLPGFDVVGRVSGA KD +PSSITL PRATLT
Sbjct: 591  MASVHATITPAVGKSGNHSYPTKSLNTAFLPGFDVVGRVSGACKDSYPSSITLTPRATLT 650

Query: 61   FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
             EP ETST KAKN KHTVDPSSPDFLPLPSFEQCFP+STKEHKE+VHEETGHVLKVPFRR
Sbjct: 651  SEPMETSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 710

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRR+KLG PRYTQMYYAKQGIITEE
Sbjct: 711  VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 770

Query: 181  MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 771  MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 830

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 831  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 890

Query: 301  NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEI RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 891  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 950

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 951  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 1010

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 1011 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 1070

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 1071 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 1130

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 1131 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 1190

Query: 601  VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
             EEAL+QGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 1191 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 1237

[Close]

BLAST of Sed0012033 vs. NCBI nr
Match: KAG6583379.1 (Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 617/647 (95.36%), Postives = 629/647 (97.22%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           MASVHATITPAVGK+GN SY TKSL+T FLPGFDVVGRVSGA KD +PSSITL PRATLT
Sbjct: 1   MASVHATITPAVGKNGNHSYPTKSLNTAFLPGFDVVGRVSGACKDSYPSSITLTPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
            EP ETST KAKN KHTVDPSSPDFLPLPSFEQCFP+STKEHKE+VHEETGHVLKVPFRR
Sbjct: 61  SEPMETSTVKAKNNKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR GLPKLRKDWVDRR+KLG PRYTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRTGLPKLRKDWVDRRDKLGAPRYTQMYYAKQGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEI RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            EEAL+QGMDAMSAEFLAAKKT+SGEQHGETGGEIYLPASY+DSLKR
Sbjct: 601 AEEALKQGMDAMSAEFLAAKKTVSGEQHGETGGEIYLPASYMDSLKR 647

[Close]

BLAST of Sed0012033 vs. NCBI nr
Match: XP_022998542.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 614/647 (94.90%), Postives = 631/647 (97.53%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           M+S+ ATITPAVGKSGNRSY+TK L + F PGFDVVGRVSGA K+LHPSSITLAPRATLT
Sbjct: 33  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 92

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKNKKHTVDPSSPDFLPLPSFE CFP+STKEHKEIVHEETGHVLKVPFRR
Sbjct: 93  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 152

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR+GLPKLRKDWVDRREKLG PR TQMYYAKQGIITEE
Sbjct: 153 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 212

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 213 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 272

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS VP+GTVPIYQALEKVNGIAE
Sbjct: 273 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 332

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTW+I RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 333 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 392

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 393 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 452

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 453 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 512

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 513 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 572

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALS+DPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 573 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 632

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           VEEALQQGMDAMSAEFL+AKKTISGEQHGETGGEIYLPASY+DS+KR
Sbjct: 633 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 679

[Close]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot
Match: O82392 (Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THIC PE=1 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 550/646 (85.14%), Postives = 594/646 (91.95%), Query Frame = 0


Query: 2   ASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  ++  LPGFDVV  V  A+      + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  EPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRRV 121
           +P  T++E+AK +KHT+DPSSPDF P+PSFE+CFP+STKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRREKLGTPRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+ RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot
Match: Q9KBJ4 (Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=thiC PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 7.3e-206
Identity = 368/583 (63.12%), Postives = 427/583 (73.24%), Query Frame = 0


Query: 90  SFEQCFPRSTKEHKEIVHEETGHVLKVPFRRVHL------SGDEPN--FDNYDTSGP--- 149
           S +  FP S K +      +    ++VPFR +        SG+  N     YD+SGP   
Sbjct: 12  SIQSSFPNSQKRYVTGSRSD----IRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTD 71

Query: 150 --QNISPRIGLPKLRKDWVDRR------------------EKLGTPR------------- 209
               ++   GLP +RK W+  R                   K   PR             
Sbjct: 72  ESYEVNIEKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHK 131

Query: 210 ---------YTQMYYAKQGIITEEMLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELE 269
                     TQM+YAK+GI+T EM F A RE + PEFVR E+ARGRAI+PSN  H E E
Sbjct: 132 PLRAKKGANVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPESE 191

Query: 270 PMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWIL 329
           PMI+GRNF VK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+
Sbjct: 192 PMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWII 251

Query: 330 RNSAVPVGTVPIYQALEKVNGIAENLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLT 389
           RNS VPVGTVPIYQALEKVNG+AE+LTWE+ R+TLIEQAEQGVDYFTIHAGVLLRYIPLT
Sbjct: 252 RNSPVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLT 311

Query: 390 AKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDA 449
           AKR+TGIVSRGGSI A+WCL HHKENF Y H+++I +I   YDI+ S+GDGLRPGSI DA
Sbjct: 312 AKRVTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADA 371

Query: 450 NDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGP 509
           ND AQFAEL T GELT+ AWE DVQVM EGPGH+PMH+I ENM KQL+ C EAPFYTLGP
Sbjct: 372 NDEAQFAELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLGP 431

Query: 510 LTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAA 569
           LTTD+APGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAA
Sbjct: 432 LTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAA 491

Query: 570 DLAKCHLHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMC 620
           DLAK H  AQ  DDALSKARFEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMC
Sbjct: 492 DLAKGHPGAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMC 551

[Close]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot
Match: A7GUZ5 (Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=thiC PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.4e-204
Identity = 363/563 (64.48%), Postives = 419/563 (74.42%), Query Frame = 0


Query: 103 KEIVHEETGHVLKVPFRRVHLSGDE--PN--FDNYDTSGP-----QNISPRIGLPKLRKD 162
           K++  E +   +KVP R + LS     PN     YDTSGP       +    G+P  R++
Sbjct: 20  KKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRN 79

Query: 163 WV----------------------------------DR---REKLGTPRYTQMYYAKQGI 222
           W+                                  DR   R K G    TQM+YA++GI
Sbjct: 80  WIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGA-NVTQMHYARKGI 139

Query: 223 ITEEMLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSA 282
           IT EM + A RE +EPEFVR E+A GRAI+P+N  H E EPMI+GRNF VKVNANIGNSA
Sbjct: 140 ITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSA 199

Query: 283 VASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVN 342
           V+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+RN+ VPVGTVPIYQALEKV 
Sbjct: 200 VSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNAPVPVGTVPIYQALEKVQ 259

Query: 343 GIAENLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL 402
           GIAENLTWE+ R+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSI A+WCL
Sbjct: 260 GIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCL 319

Query: 403 AHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAW 462
            HHKENF Y H+++I +I  QYD+S S+GDGLRPGSI DAND AQFAEL T GELT+ AW
Sbjct: 320 YHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAW 379

Query: 463 EKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAAN 522
           + DVQVM EGPGH+PMH I ENM+K+++ C  APFYTLGPLTTD+APGYDHITSAIGAA 
Sbjct: 380 KHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAM 439

Query: 523 IGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKAR 582
           IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAADLAK H  AQ  DDALSKAR
Sbjct: 440 IGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGHKTAQQRDDALSKAR 499

Query: 583 FEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEH 620
           FEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMCGPKFCSMKI+ D+R+YA+E+
Sbjct: 500 FEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKEN 559

[Close]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot
Match: Q5YNS3 (Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=thiC PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.5e-203
Identity = 354/530 (66.79%), Postives = 414/530 (78.11%), Query Frame = 0


Query: 114 LKVPFRRVHLSGDEPNFDNYDTSGP-----QNISPRIGLPKLRKDWVDRREKLGTPRYTQ 173
           L++P RR++L+  E  FD YDTSGP       I    GLPKLR  W   +  +  PR TQ
Sbjct: 32  LRIPVRRINLTNGE-TFDVYDTSGPYTDENATIDLEAGLPKLRDSW--DKPTVAGPR-TQ 91

Query: 174 MYYAKQGIITEEMLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKV 233
           + +A+ GI+T EM F A RE +EPE VR+EVA GRA+IP+N  H ELEP I+G+ FLVK+
Sbjct: 92  LAWARAGIVTPEMRFIAAREGVEPELVRAEVAAGRAVIPANHNHPELEPTIIGKKFLVKI 151

Query: 234 NANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPI 293
           NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IHETREWILRNS VPVGTVPI
Sbjct: 152 NANIGNSAVSSSIAEEVEKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPI 211

Query: 294 YQALEKVNGIAENLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGG 353
           YQALEKVNG    LTWEI R+T+IEQAEQGVDY T+HAGVLLRY+PLTAKR+TGIVSRGG
Sbjct: 212 YQALEKVNGDPAALTWEIYRDTVIEQAEQGVDYMTVHAGVLLRYVPLTAKRVTGIVSRGG 271

Query: 354 SIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQ 413
           SI A WCLAHH+E+F Y H++++ +I  +YD++ S+GDGLRPGS+ DAND AQFAEL T 
Sbjct: 272 SIMAAWCLAHHRESFLYTHFEELCEILARYDVTFSLGDGLRPGSVADANDEAQFAELRTL 331

Query: 414 GELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHI 473
           GELT+ A    VQVM EGPGH+PMHKI EN++ + E C EAPFYTLGPL TD+AP YDHI
Sbjct: 332 GELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHI 391

Query: 474 TSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSW 533
           TSAIGAA I   GTA+LCYVTPKEHLGLPNRDDVK GVI YKIAAHAADLAK H HAQ  
Sbjct: 392 TSAIGAAIIAQAGTAMLCYVTPKEHLGLPNRDDVKTGVITYKIAAHAADLAKGHPHAQQR 451

Query: 534 DDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITED 593
           DDALSKARFEFRW DQFALSLDP TA  +HDET+P+E AK AHFCSMCGPKFCSM+I+ D
Sbjct: 452 DDALSKARFEFRWRDQFALSLDPDTAREYHDETMPAEPAKTAHFCSMCGPKFCSMRISAD 511

Query: 594 VRKYAEEHGYGNVEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLP 639
           VR+YAE +   +V  A++ GM A SAEF             E+GG++YLP
Sbjct: 512 VREYAEANNLTDV-AAIEAGMAAKSAEF------------AESGGKVYLP 544

[Close]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot
Match: A4VR38 (Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=thiC PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 7.6e-203
Identity = 367/600 (61.17%), Postives = 422/600 (70.33%), Query Frame = 0


Query: 93  QCFPRSTKEHKEIVHEETGHVLKVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 152
           Q FPRS K    I  + +   ++VP R + L     +F          YDTSGP      
Sbjct: 21  QPFPRSQK----IYVQGSRPDIRVPMREISLDVTPTDFGGEINAPVTVYDTSGPYTDPNV 80

Query: 153 NISPRIGLPKLRKDWVDRR---EKL-------------------------GTPR------ 212
            I  R GL  +R  W++ R   EKL                           PR      
Sbjct: 81  TIDVRKGLADVRSAWIEDRGDTEKLPGLTSEFGQRRLNDAELSAMRFAHVRNPRRAKAEH 140

Query: 213 -YTQMYYAKQGIITEEMLFCANREKLE----------------------------PEFVR 272
             +QM+YAK+GIIT EM + A RE ++                            PEFVR
Sbjct: 141 NVSQMHYAKKGIITPEMEYVAIRENMKLAEAREAGLLVEQHAGQSFGAAIPKEITPEFVR 200

Query: 273 SEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGA 332
           SEVARGRAIIP+N  H ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WG+
Sbjct: 201 SEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGS 260

Query: 333 DTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAENLTWEILRETLIEQAE 392
           DTVMDLSTG+HIHETREWI+RNS VP+GTVPIYQALEKVNG+AE+LTWE+ R+TLIEQAE
Sbjct: 261 DTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAE 320

Query: 393 QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICN 452
           QGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I  
Sbjct: 321 QGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMK 380

Query: 453 QYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIP 512
            YD+S S+GDGLRPGSI DAND AQF EL T GELT+ AW+ DVQ M EGPGH+PMH I 
Sbjct: 381 AYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMHMIK 440

Query: 513 ENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGL 572
           ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGL
Sbjct: 441 ENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGL 500

Query: 573 PNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRWMDQFALSLDPMTAMS 617
           PN+DDVK G+I YKIAAHAADLAK H  AQ  D+ALSKARFEFRW DQF L LDP TA +
Sbjct: 501 PNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARA 560

[Close]

BLAST of Sed0012033 vs. ExPASy TrEMBL
Match: A0A6J1KCT8 (phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111493145 PE=3 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 614/647 (94.90%), Postives = 631/647 (97.53%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           M+S+ ATITPAVGKSGNRSY+TK L + F PGFDVVGRVSGA K+LHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKNKKHTVDPSSPDFLPLPSFE CFP+STKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR+GLPKLRKDWVDRREKLG PR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS VP+GTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTW+I RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALS+DPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           VEEALQQGMDAMSAEFL+AKKTISGEQHGETGGEIYLPASY+DS+KR
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 647

[Close]

BLAST of Sed0012033 vs. ExPASy TrEMBL
Match: A0A6J1KAG1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493145 PE=3 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 614/647 (94.90%), Postives = 631/647 (97.53%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           M+S+ ATITPAVGKSGNRSY+TK L + F PGFDVVGRVSGA K+LHPSSITLAPRATLT
Sbjct: 33  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 92

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKNKKHTVDPSSPDFLPLPSFE CFP+STKEHKEIVHEETGHVLKVPFRR
Sbjct: 93  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 152

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR+GLPKLRKDWVDRREKLG PR TQMYYAKQGIITEE
Sbjct: 153 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 212

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 213 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 272

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS VP+GTVPIYQALEKVNGIAE
Sbjct: 273 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 332

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTW+I RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 333 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 392

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 393 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 452

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 453 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 512

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 513 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 572

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALS+DPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 573 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 632

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           VEEALQQGMDAMSAEFL+AKKTISGEQHGETGGEIYLPASY+DS+KR
Sbjct: 633 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 679

[Close]

BLAST of Sed0012033 vs. ExPASy TrEMBL
Match: A0A6J1K8A0 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493145 PE=3 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 614/647 (94.90%), Postives = 631/647 (97.53%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           M+S+ ATITPAVGKSGNRSY+TK L + F PGFDVVGRVSGA K+LHPSSITLAPRATLT
Sbjct: 33  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 92

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKNKKHTVDPSSPDFLPLPSFE CFP+STKEHKEIVHEETGHVLKVPFRR
Sbjct: 93  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 152

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPR+GLPKLRKDWVDRREKLG PR TQMYYAKQGIITEE
Sbjct: 153 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 212

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 213 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 272

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS VP+GTVPIYQALEKVNGIAE
Sbjct: 273 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 332

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTW+I RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 333 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 392

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 393 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 452

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 453 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 512

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 513 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 572

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALS+DPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 573 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 632

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           VEEALQQGMDAMSAEFL+AKKTISGEQHGETGGEIYLPASY+DS+KR
Sbjct: 633 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 679

[Close]

BLAST of Sed0012033 vs. ExPASy TrEMBL
Match: A0A6J1GCE0 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452695 PE=3 SV=1)

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 613/647 (94.74%), Postives = 631/647 (97.53%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           M+S+ ATITPAVGKSGNRSY+TK L + F PGFDVVGRVSGA K+LHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKNKKHTVDPSSPDFLPLPSFE CFP+STKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEP+FDNYDTSGPQNISPR+GLPKLRKDWVDRREKLG PR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS VP+GTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTW+I RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALS+DPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           VEEALQQGMDAMSAEFL+AKKTISGEQHGETGGEIYLPASY+DS+KR
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 647

[Close]

BLAST of Sed0012033 vs. ExPASy TrEMBL
Match: A0A5A7SPA0 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00490 PE=3 SV=1)

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 617/647 (95.36%), Postives = 629/647 (97.22%), Query Frame = 0


Query: 1   MASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLT 60
           MASVHATITPAVGKS NRS  TKSL+T FLPGFDVVGRV+ A KDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATLT 60

Query: 61  FEPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRR 120
           F+P ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFP+STKEHKE+VHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDWVDRR+KLG PR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCA REKL+PEFVRSEVARGRAIIPSNK HLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEI RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG 
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 VEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
            EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASY++S KR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of Sed0012033 vs. TAIR 10
Match: AT2G29630.2 (thiaminC )

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 550/646 (85.14%), Postives = 594/646 (91.95%), Query Frame = 0


Query: 2   ASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  ++  LPGFDVV  V  A+      + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  EPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRRV 121
           +P  T++E+AK +KHT+DPSSPDF P+PSFE+CFP+STKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRREKLGTPRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+ RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Sed0012033 vs. TAIR 10
Match: AT2G29630.1 (thiaminC )

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 550/646 (85.14%), Postives = 594/646 (91.95%), Query Frame = 0


Query: 2   ASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  ++  LPGFDVV  V  A+      + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  EPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRRV 121
           +P  T++E+AK +KHT+DPSSPDF P+PSFE+CFP+STKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRREKLGTPRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+ RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of Sed0012033 vs. TAIR 10
Match: AT2G29630.3 (thiaminC )

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 550/646 (85.14%), Postives = 594/646 (91.95%), Query Frame = 0


Query: 2   ASVHATITPAVGKSGNRSYATKSLSTVFLPGFDVVGRVSGASKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  ++  LPGFDVV  V  A+      + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  EPSETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPRSTKEHKEIVHEETGHVLKVPFRRV 121
           +P  T++E+AK +KHT+DPSSPDF P+PSFE+CFP+STKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+W+DRREKLGTPRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCANREKLEPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CA REKL+PEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEILRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+ RETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKCHLHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK H HAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYLDSLKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SY+ + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

The following BLAST results are available for this feature:

BLAST of Sed0012033 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894338.1	0.0e+00	96.14	phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida] >...	[more]
XP_038894337.1	0.0e+00	96.14	phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_023519726.1	0.0e+00	95.52	phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 [Cucurbita pepo ...	[more]
KAG6583379.1	0.0e+00	95.36	Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_022998542.1	0.0e+00	94.90	phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucurbita maxima]	[more]

BLAST of Sed0012033 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82392	0.0e+00	85.14	Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9KBJ4	7.3e-206	63.12	Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / D...	[more]
A7GUZ5	2.4e-204	64.48	Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP...	[more]
Q5YNS3	1.5e-203	66.79	Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247...	[more]
A4VR38	7.6e-203	61.17	Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=37973...	[more]

BLAST of Sed0012033 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KCT8	0.0e+00	94.90	phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucurbita maxima O...	[more]
A0A6J1KAG1	0.0e+00	94.90	phosphomethylpyrimidine synthase, chloroplastic isoform X1 OS=Cucurbita maxima O...	[more]
A0A6J1K8A0	0.0e+00	94.90	phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucurbita maxima O...	[more]
A0A6J1GCE0	0.0e+00	94.74	phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita mos...	[more]
A0A5A7SPA0	0.0e+00	95.36	Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of Sed0012033 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29630.2	0.0e+00	85.14	thiaminC	[more]
AT2G29630.1	0.0e+00	85.14	thiaminC	[more]
AT2G29630.3	0.0e+00	85.14	thiaminC	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038521	ThiC/Bza, core domain	GENE3D	3.20.20.540	Radical SAM ThiC family, central domain	coord: 209..523 e-value: 3.9E-167 score: 556.8
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	TIGRFAM	TIGR00190	TIGR00190	coord: 167..590 e-value: 3.2E-202 score: 669.8
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PFAM	PF01964	ThiC_Rad_SAM	coord: 167..587 e-value: 6.3E-196 score: 651.1
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	SFLD	SFLDF00407	phosphomethylpyrimidine_synth	coord: 167..587 e-value: 0.0 score: 652.1
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PANTHER	PTHR30557	THIAMINE BIOSYNTHESIS PROTEIN THIC	coord: 1..647
None	No IPR available	GENE3D	6.10.250.620		coord: 527..559 e-value: 3.9E-18 score: 67.0
None	No IPR available	SFLD	SFLDS00113	Radical_SAM_Phosphomethylpyri	coord: 164..623 e-value: 0.0 score: 849.6
None	No IPR available	SFLD	SFLDG01114	phosphomethylpyrimidine_synth	coord: 165..621 e-value: 0.0 score: 830.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..83
None	No IPR available	PANTHER	PTHR30557:SF2	SUBFAMILY NOT NAMED	coord: 1..647
IPR037509	Phosphomethylpyrimidine synthase	HAMAP	MF_00089	ThiC	coord: 166..592 score: 93.919029

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0012033.1	Sed0012033.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016830	carbon-carbon lyase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding