Sed0012026 (gene) Chayote v1

BLAST of Sed0012026 vs. NCBI nr
Match: XP_004139639.1 (vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >XP_011659140.1 vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >KGN44805.2 hypothetical protein Csa_015985 [Cucumis sativus])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 718/781 (91.93%), Postives = 740/781 (94.75%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME EDVPMDEKAKRMRDLLSSFYSPD SMSGSPT SSNRYASPLEAIN+TSFNPDQYM+I
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+  L   SS +GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTTALVNASSKDGNSSELVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EF EAVRAYRVIFADSD+QL KLA DLVTKHFDSTEQFIKKQICAAD L VFGIIWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LL GEVLNDA L DYSLKAAQV+VKQ+V  TFSRLLQ+ISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLFGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           L LEASKKAVLQGSMDVLL+FRQLLEDQSGLIINQRDSIVDWVQEGFQ+FF ALVD FML
Sbjct: 421 LELEASKKAVLQGSMDVLLEFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQ LTEATQA+KV+AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVK+KEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKYKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNSA 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN  
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 T 782
           T
Sbjct: 781 T 781

BLAST of Sed0012026 vs. NCBI nr
Match: XP_038887792.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887793.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887794.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887795.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887796.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 715/779 (91.78%), Postives = 739/779 (94.87%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME +DVPMDEKAKRMRDLLSSFY+PD SMS S   SSNRY SPLEAIN+TSFNPDQYMSI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYAPDASMSSSSMGSSNRYTSPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEE IA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEVIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQ+NAEDLS  L  GSS +G+ SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQVNAEDLSSALVNGSSKDGSTSELVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEAVRAYRVIFADSD+QL KLA DLVTKHFD+TEQFIKKQICAAD L VFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA LPDYSLKAAQV+VKQ+VK  FSRLLQ+ISDALTQVHTRKKEG QEYSLQ
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVKCKFSRLLQDISDALTQVHTRKKEGAQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           LALEA+KKAVLQGSMDVLLDFRQLLED+SGLIINQRDSIVDWVQEGFQ+FF ALVD FML
Sbjct: 421 LALEATKKAVLQGSMDVLLDFRQLLEDKSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQVLTEATQA+KV+AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYSQSQVLTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRIS+L  KRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISILSTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVR QTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTLVKLSLKTLQEFVRFQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNS 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQNS
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNS 779

BLAST of Sed0012026 vs. NCBI nr
Match: XP_008461504.1 (PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo] >XP_008461505.1 PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo])

HSP 1 Score: 1346.6 bits (3484), Expect = 0.0e+00
Identity = 717/781 (91.81%), Postives = 738/781 (94.49%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME +DVPMDEKAKRMRDLLSSFYSPD SMS SPT SSNRYASPLEAIN+TSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+  L   SS +GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EF EAVRAYRVIFADSD+QL KLA DLVTKHFDSTEQFIKKQICAAD L VFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQV+VKQ+V  TFSRLLQ+ISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           L LEASKKAVLQGSMD+LLDFRQLLEDQSGLIINQRDSIVDWVQEGFQ+FF ALVD FML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQ LTEATQA+KV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNSA 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN  
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 T 782
           T
Sbjct: 781 T 781

BLAST of Sed0012026 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1337.8 bits (3461), Expect = 0.0e+00
Identity = 711/779 (91.27%), Postives = 738/779 (94.74%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME ++ PMDEK KRMRDLLSSFYSPD S SGS   SSNRYASPLEAIN+TSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFK YGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLS  L IGSS +GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEA+RAYRVIFADSD+QL KLA DLVTKHFD+ EQFI+KQICAAD L VFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQV+VKQ+V  TFSRLLQ+ISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGL I+QRDSIVDWVQEGFQ+FF ALVD FM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSG N+S+ QSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           RGYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQN 779
           MQFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQN 779

BLAST of Sed0012026 vs. NCBI nr
Match: XP_022929381.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929382.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929383.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929384.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 712/779 (91.40%), Postives = 738/779 (94.74%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME ++ PMDEK KRMRDLLSSFYSPD S SGS   SSNRYASPLEAIN+TSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLL KVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFK YGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLS  L IGSS +GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEA+RAYRVIFADSD+QL KLA DLVTKHFD+TEQFI+KQICAAD L VFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQV+VKQ+V  TFSRLLQ+ISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGL I+QRDSIVDWVQEGFQ+FF ALVD FM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSG N+S+ QSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           RGYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQN 779
           MQFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQN 779

BLAST of Sed0012026 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 1.2e-282
Identity = 527/785 (67.13%), Postives = 631/785 (80.38%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           M TE  PMDEKAKRMRDLLSSFY+PD S+S   TS S+  AS  + INSTSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSIS---TSGSSINAS-FDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAE+KNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           +QLL+KI+SVQS+SDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
            DAVR+YTGAMPI K YGD+SFQDC+RASEEAI  ++K LQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGAS-HEAS 300
           LKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S ++    S     S   + A  HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VQEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTD 360
           V+ F+EA+RAYR IF DS+++L KLA  L   HF+  E +IKK++ AADFL +F I+W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV++KQFV   FS L Q+ISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           ++ LEAS+KAVLQG+ ++  DFRQLL++++G+ I  +D I  W+Q+G Q+FF +L   F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSG  +S        E   +DK+ AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL+KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGT-RKHRRTDSNGS--TTSSRSNPLREEKLSRSNTQR 660
           M+VD+FL ELE VG EVKQ+LPQGT RKH+RTDSNGS  TTSSRSN L  +K++RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL E+HLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKD 780
           QLD+QFL+ PLKE  +DEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAK+K+
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 777

Query: 781 QNSAT 782
            N+ T
Sbjct: 781 HNNNT 777

BLAST of Sed0012026 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.6e-69
Identity = 223/803 (27.77%), Postives = 399/803 (49.69%), Query Frame = 0

Query: 11  KAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSILVQKSNLEGL 70
           ++KR+R+LL ++Y P +   GS +SS N    PL  I+  SFN + Y   +V+ S L  L
Sbjct: 147 ESKRVRNLLKTYYGPGIG-EGSDSSSMN---DPLN-IDGPSFNLNSYFDSIVKSSTLNQL 206

Query: 71  LQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSV 130
           +Q+  +M +E++ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + +  +
Sbjct: 207 IQKDNQMVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLI 266

Query: 131 QSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAYADAVRYYTGA 190
            + S+ +N++L  +R+ I++    +   +K+QF+  LP+ L  C+  +AY  AVRYY   
Sbjct: 267 TNCSEKINSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSN 326

Query: 191 MPIFKAYGD-SSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 250
             I K Y    SFQ+ +   +  + T+  KL E+L S S S     E+A +L  L  PV+
Sbjct: 327 SGILKQYSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVE 386

Query: 251 SLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASVQEFAEAVRA 310
            +++K LE  +  T+ L  N E  S         E N +           + E++  + +
Sbjct: 387 LVRSKYLESRKHHTITLLENLEKKSVEHITDYIKELNAN----------FLSEYSYNITS 446

Query: 311 YRVIF------ADSDK-------QLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGII 370
           Y+ +F      +DS K       QL   + DL  K+ +  +  +       + +    II
Sbjct: 447 YKSLFINRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALEII 506

Query: 371 WTDVLLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHT----RKKE 430
            +DV  LG  L+     +        +V   +   F  L + I + + Q+++    R+ E
Sbjct: 507 NSDVSRLGSELSS---HEKITNIINSTVHDQIDFYFDNLQKTIKEHIHQLNSTLNERRDE 566

Query: 431 GVQEYSLQLALEASKKAVLQGSMDVLLDFRQL----LEDQSGLIINQRDSIVDWVQEGFQ 490
            ++ ++LQ   +A+ KA++    D++L F  L    L  ++  + + +D+I   +Q   Q
Sbjct: 567 VLEGHNLQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVKLQ 626

Query: 491 EFFSALVD-HFMLLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRIT 550
           +FF  LV+ HF+             ++   +  ++     +LVL+ I ++ E   I  + 
Sbjct: 627 QFFLFLVNIHFL---------EYLDIIATTSNREQFSGRFLLVLSSICLYFENKGITLVV 686

Query: 551 EEIA--ASFSGGGIRGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFR 610
           + ++   + +  G +      +F   ++C+  R  G + LN++  + SQ++  +LKK   
Sbjct: 687 QLMSEFITVAKQGAKKELNVFSFNAPDLCKRVRETGLQILNVFTRLSSQKLDKILKKGLE 746

Query: 611 T---PNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILP------QGTRKHRRTDSNGST 670
           +    NW+  KEPR+V    D++L+E+    +E  ++LP         + H RT S G++
Sbjct: 747 SIPNNNWLVLKEPRDVRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNS 806

Query: 671 TSSRSNPLREEKLSRSNTQRARSQLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPL 730
            SS ++        R N   + S    +    LF++K++    V+F   SV+   +KL L
Sbjct: 807 VSSNNSSSNTPDGHRRNPSASSSSSSNNTNTTLFEKKVD---HVDFNTLSVLIAIIKLSL 866

Query: 731 KTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPI 780
           K+  E +RL+TF  +G  QIQ+D+ +L+  L ++    +  D LL E     +ERC+DP+
Sbjct: 867 KSFNESLRLKTFGTNGCHQIQIDLHYLKLSLYDLFGSNS-FDNLLQECENTVTERCVDPL 915

BLAST of Sed0012026 vs. ExPASy Swiss-Prot
Match: Q155U0 (Vacuolar protein sorting-associated protein 51 homolog OS=Danio rerio OX=7955 GN=vps51 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.1e-58
Identity = 218/843 (25.86%), Postives = 388/843 (46.03%), Query Frame = 0

Query: 1   METEDVPMDE---KAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQY 60
           M +   P D    + +R+  +L  +Y   ++  G  T  +     P + IN   F+P+ Y
Sbjct: 1   MSSATTPPDSDPAQRRRVHSMLKLYYG--LNEEGKATEQAES-LDPCD-INGPHFDPEIY 60

Query: 61  MSILVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGME 120
           ++ L ++ +L  L+     M  ++++LD+D+Q LVYENYNKFISATDTI++M N+   ME
Sbjct: 61  LNKLRKECSLTELMDHESCMVKQIRSLDSDMQTLVYENYNKFISATDTIRKMKNDFKKME 120

Query: 121 TNMEQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKA 180
             M+ L   + ++   S  ++ +L ++   I K      LLRK+QF+++LPARL KC++ 
Sbjct: 121 DEMDCLSANMAAITEFSARISGTLQDQHAQITKLSGVHTLLRKLQFLFELPARLNKCLEL 180

Query: 181 EAYADAVRYYTGAMPIFKAYGD-SSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAE 240
           +AYA AV  +  A  + + Y    SF+  +      +  + ++L++K      S +  +E
Sbjct: 181 QAYAQAVSSHRRARCVLQQYSHMPSFRGIQDDCHVIMEQLAQQLRQKFRDGGSSAKDLSE 240

Query: 241 AAVLLKQLDFPVDSLKAKLL----EKLEQSTLDLQLNAEDLSRVLAIGS----------- 300
              LL QLD P + L  K L     +LE     L+   +D S V   G+           
Sbjct: 241 CVELLLQLDEPAEELCDKFLSHAQSRLEADLQGLEAELKD-SAVTDTGAGSVQKTSPGSN 300

Query: 301 --SMEGNVSESVYGASHEASVQEFAE------------AVRAYRVIFAD-------SDKQ 360
             S   +VS      +    + EF +             + +Y+ +F +       + K 
Sbjct: 301 PVSPSSSVSNPFLSPAAGTDILEFIDRGCNEFVSNLCLVIASYQELFINRPQESELASKN 360

Query: 361 LSKLAL--------DLVTKHFDSTEQFIKKQICAAD---FLHVFGIIWTDVLLLGEVLND 420
           + ++A          L  ++F   E+ I+++    D    +         +  + ++L  
Sbjct: 361 IPEMANGKLHVFVDTLAARYFSLVERRIQEEKGVGDNSLLVRALDRFHRRLQAISKLLPG 420

Query: 421 ACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQLALEASKKA 480
           + +P    +    + ++ +K   S L     D+LT V  R+       S+  A  +   A
Sbjct: 421 SAVPSQGTEIVVRAARERIKQYLSALQTFYHDSLTDV--RQALAAPRLSVGGASASGGGA 480

Query: 481 VLQG--SMDVLLDFRQLLEDQSGLIINQRDSIV---------------------DWVQEG 540
           ++ G  S D      +LL   S  I+NQ  S++                     ++  +G
Sbjct: 481 LVGGASSKDAPPSLPELLTSLSNFILNQLKSVLASVHLFTAKDITFSNKPYFKGEFCSQG 540

Query: 541 FQEFFSALVDHFMLLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRI 600
            +E    +V     +  ++  Y +S       +       L+L+L+++ +  E + I  I
Sbjct: 541 VRE--GLVVSFIKFICQSSRQYCES----AGDRGGSTPPALLLLLSRLCLDYETSTISYI 600

Query: 601 TEEIAASFSGGGIRGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRT 660
                  F         + P      +C   R A +K LN Y+ ++   IS +L+K   T
Sbjct: 601 LTLTDEQF-----LVQHHTPVTPVTALCAEAREAAQKLLNHYVKVQGLIISQMLRKSVET 660

Query: 661 PNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLRE 720
            +WV   EPR V   +   +++  ++  +V  +  +G RK   +DS+  T S  S+  ++
Sbjct: 661 RDWVNTIEPRNVRAVMKRVVEDTTSIDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRQQ 720

Query: 721 EKLSRSNTQRARSQL-LESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRL 769
            + + S T  A     L S++ KLF ++I+IF+ VEF + SV+T  +K+ LKT  E VRL
Sbjct: 721 IRYAPSYTPSAPMDTNLLSNIHKLFSERIDIFSPVEFNKVSVLTGIIKISLKTFLECVRL 780

BLAST of Sed0012026 vs. ExPASy Swiss-Prot
Match: Q4V9Y0 (Vacuolar protein sorting-associated protein 51 homolog OS=Xenopus tropicalis OX=8364 GN=vps51 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.2e-56
Identity = 202/749 (26.97%), Postives = 361/749 (48.20%), Query Frame = 0

Query: 39  RYASPLEAINSTSFNPDQYMSILVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNK 98
           R+  P + ++   FNP+ Y++ L ++S+L  L+    +M  ++++LD+++Q LVYENYNK
Sbjct: 37  RHLEPTD-VDGVHFNPELYLTKLRKESSLSQLMDVEADMVRQIRSLDSEMQTLVYENYNK 96

Query: 99  FISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLL 158
           FISATDTI++M N+   ME  M+ L   +  +   S  ++++L E+ + I K      LL
Sbjct: 97  FISATDTIRKMKNDFKKMEDEMDGLATNMAVITEFSARISSTLQERHQQITKLSGVHTLL 156

Query: 159 RKVQFIYDLPARLGKCIKAEAYADAVRYYTGAMPIFKAYGD-SSFQDCKRASEEAIATVL 218
           RK+QF+++LPARL KCI+  AYA AV Y++ A  +   Y    SF   +   +  +A + 
Sbjct: 157 RKLQFLFELPARLKKCIELGAYAQAVSYHSKARSVLHQYQHMPSFHGIQTDCQAIMAGLA 216

Query: 219 KKLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLE----KLEQSTLDLQLNAEDL 278
             L+++    + S Q  +E   +L  L+ P   L  + L     +L     DLQ + + L
Sbjct: 217 DTLRQRFRDPASSPQDLSECVEMLLNLEEPAHLLCDEFLAHGRGRLASHLSDLQESGDIL 276

Query: 279 SRVLAIGSSMEGNVSES-VYGASHEASVQEFAEAVRAYRVIFADSDKQLSKLALDLVTKH 338
                +     G +S++ +  AS+++   + A +          ++ +L+    +L T +
Sbjct: 277 E---FVDRGCGGFISDACLLAASYQSLFSKEAGSTAQM------AEAKLTSFLEELSTGY 336

Query: 339 FDSTEQFIKKQICAADFLHVFGII--WTDVLLLGEVLNDAC-----LPDYSLKAAQVSVK 398
           F+  E+ ++++    D   +   +  +   L     L   C       +  ++AAQ  + 
Sbjct: 337 FELVEKRLRQEKSLGDNSLLVRALDRFHRRLQAPSKLVPGCGFNRRGTEIVVRAAQERLA 396

Query: 399 QF---VKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQLALEASKKAVLQGSMDVLLDFR 458
           Q+   +K  F   L ++  AL       KE      L   L AS   VL     VL    
Sbjct: 397 QYLQALKDFFQGCLTDVRQALAAPRLPGKEMPALGDLLAGLSAS---VLNQIKTVLAAVH 456

Query: 459 QLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFMLLSGNNNSYGQSQVLTE--ATQA 518
                    +   +D           EF S  V   ++++   +    ++   E    + 
Sbjct: 457 ---------LFTAKDVAFSDKPYFKGEFCSQGVREGLIVAFIKSVCQTARQFCEIPGEKG 516

Query: 519 DKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFLPAEICRMFRAA 578
                 L+L+L+++ +  E + I  I       F      G ++ P    + +C + R+ 
Sbjct: 517 TSTPPALLLLLSRLCLDYETSTISYILTLTDEQF-----LGQDHSPVTPVSSLCSLARST 576

Query: 579 GEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQIL 638
            +  LN Y+  +   +S +L+K   T +WV   EPR V   +   ++++  V  +V  + 
Sbjct: 577 AQTLLNQYVKSQGLVVSQMLRKSVETRDWVTTIEPRNVRAVMKRVVEDITGVDVQVGLLY 636

Query: 639 PQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQL-LESHLAKLFKQKIEIFTR 698
            +G RK   +DS+  T S  S+   + + ++S T  A     L S++ KLF ++I+IF+ 
Sbjct: 637 EEGVRKAHSSDSSKRTFSVYSSSRLQGRYAQSYTPSAPMDTNLLSNIQKLFSERIDIFST 696

Query: 699 VEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAVIDF 758
           V+F + S++T  +K+ LKT  E VRL+TF R G QQIQ+D  +L+  L     DE ++  
Sbjct: 697 VQFNKVSILTGIIKISLKTFLECVRLRTFGRYGLQQIQVDCHYLQLYLWRFVSDENLVHC 756

Query: 759 LLDEVIVAASERCLDPIPLEPPILDKLIQ 769
           LLDEV+ +A+ RCLDP P+E  +++ + +
Sbjct: 757 LLDEVVGSAAHRCLDPAPMEQSVIEVICE 758

BLAST of Sed0012026 vs. ExPASy Swiss-Prot
Match: Q9UID3 (Vacuolar protein sorting-associated protein 51 homolog OS=Homo sapiens OX=9606 GN=VPS51 PE=1 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 4.9e-55
Identity = 201/777 (25.87%), Postives = 361/777 (46.46%), Query Frame = 0

Query: 10  EKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEA--INSTSFNPDQYMSILVQKSNL 69
           E+ ++   +L  +Y     +S    +       PL+   +N   F+P+ Y+  L ++  L
Sbjct: 28  ERRRKAHGMLKLYY----GLSEGEAAGRPAGPDPLDPTDLNGAHFDPEVYLDKLRRECPL 87

Query: 70  EGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKI 129
             L+    +M  +++ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   +
Sbjct: 88  AQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNM 147

Query: 130 LSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAYADAVRYY 189
             +   S  ++ +L ++ E I K      LLRK+QF+++LP+RL KC++  AY  AVRY 
Sbjct: 148 AVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQ 207

Query: 190 TGAMPIFKAYGD-SSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVLLKQLDF 249
             A  + + Y    SF+  +   +   A + ++L+++          +AE   LL  L  
Sbjct: 208 GRAQAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELLLALGE 267

Query: 250 PVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASVQEFAEA 309
           P + L     E L  +   L+    +L   L         +  + +G S    V    + 
Sbjct: 268 PAEEL---CEEFLAHARGRLEKELRNLEAELGPSPPAPDVLEFTDHGGS--GFVGGLCQV 327

Query: 310 VRAYRVIFADSD----KQLSKLALDLVTKHFDSTEQFIKKQICAAD---FLHVFGIIWTD 369
             AY+ +FA       ++L+  A  L +++F   E+ + ++    D    +         
Sbjct: 328 AAAYQELFAAQGPAGAEKLAAFARQLGSRYFALVERRLAQEQGGGDNSLLVRALDRFHRR 387

Query: 370 VLLLGEVLNDACLPDYSLKAAQVSVK-------QFVKSTFSRLLQEISDALTQVHTRKKE 429
           +   G +L  A L D + +  +   +       Q +++ F   L ++  AL       KE
Sbjct: 388 LRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLGCLTDVRQALAAPRVAGKE 447

Query: 430 GVQEYSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFS 489
           G     L   + +S  + ++ S+  +     L   +     N+     ++  +G +E   
Sbjct: 448 GPGLAELLANVASSILSHIKASLAAV----HLFTAKEVSFSNKPYFRGEFCSQGVREGLI 507

Query: 490 ALVDHFMLLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAA 549
               H M  +       QS   +   +       L+L+L+++ +  E   I  I      
Sbjct: 508 VGFVHSMCQT------AQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDE 567

Query: 550 SFSGGGIRGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKH 609
            F        +  P    + +C   R    + L  Y+ ++   IS +L+K   T +W+  
Sbjct: 568 QF-----LVQDQFPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLST 627

Query: 610 KEPREVHMFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRS 669
            EPR V   +   +++  A+  +V  +  +G RK + +DS+  T S  S+  ++ + + S
Sbjct: 628 LEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPS 687

Query: 670 NTQRARSQL-LESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRS 729
            T  A     L S++ KLF ++I++F+ VEF + SV+T  +K+ LKTL E VRL+TF R 
Sbjct: 688 YTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRTFGRF 747

Query: 730 GFQQIQLDMQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQ 769
           G QQ+Q+D  FL+  L     DE ++  LLDEV+ +A+ RC DP+P+EP +++ + +
Sbjct: 748 GLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVICE 780

BLAST of Sed0012026 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1346.6 bits (3484), Expect = 0.0e+00
Identity = 717/781 (91.81%), Postives = 738/781 (94.49%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME +DVPMDEKAKRMRDLLSSFYSPD SMS SPT SSNRYASPLEAIN+TSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+  L   SS +GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EF EAVRAYRVIFADSD+QL KLA DLVTKHFDSTEQFIKKQICAAD L VFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQV+VKQ+V  TFSRLLQ+ISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           L LEASKKAVLQGSMD+LLDFRQLLEDQSGLIINQRDSIVDWVQEGFQ+FF ALVD FML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQ LTEATQA+KV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNSA 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN  
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 T 782
           T
Sbjct: 781 T 781

BLAST of Sed0012026 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 712/779 (91.40%), Postives = 738/779 (94.74%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME ++ PMDEK KRMRDLLSSFYSPD S SGS   SSNRYASPLEAIN+TSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLL KVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFK YGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLS  L IGSS +GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEA+RAYRVIFADSD+QL KLA DLVTKHFD+TEQFI+KQICAAD L VFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQV+VKQ+V  TFSRLLQ+ISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGL I+QRDSIVDWVQEGFQ+FF ALVD FM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSG N+S+ QSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           RGYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQN 779
           MQFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAKAKDQN
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQN 779

BLAST of Sed0012026 vs. ExPASy TrEMBL
Match: A0A6J1GBC0 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111452610 PE=3 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 711/779 (91.27%), Postives = 735/779 (94.35%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME EDVPMDEKAKRMRDLLSSFYSPD SMSGS T SSNRYASPLE IN+TSFNPDQYMSI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEEAIA VLKKLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNA+DLS  L I S  E N SES YGA+HEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGAAHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEAVRAYRVIFADSD QL KLA DLVTKHFD+ EQFIKKQI AAD L VFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDMQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDACL DYSLKAAQV+VKQ+V  TFSRLLQ ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           LALEA KKAVLQGSMDVLLDFR LLE+QSGLIINQRDSIVDWVQEGFQ+FF ALVD FML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQVLTEATQADKVVAGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+QIL QGTRKHRRT+SNGSTTSSRS PLREEKL+++N QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNKTNAQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNS 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLDPIPLEPPILDKLIQ KLAKA+DQNS
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 779

BLAST of Sed0012026 vs. ExPASy TrEMBL
Match: A0A6J1K9H7 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492889 PE=3 SV=1)

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 711/779 (91.27%), Postives = 734/779 (94.22%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME EDVPMDEKAKRMRDLLSSFYSPD SMSGS T SSNRYASPLE IN+TSFNPDQYMSI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NLEGLLQRHVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFKAYGDSSFQDCKRASEEAIA VLKKLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNA+DLS  L I S  E N SES YGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGASHEASV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEAVRAYRVIFADSD+QL KLA DLVTKHFD+ EQFIKKQI AAD L VFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDACL DYSLKAAQV+VKQ+V  TFSRLLQ ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFML 480
           LALEA KKAVLQGSMDVLLDFR LLE+QSGLIINQRDSIVDWVQEGFQ+FF  LVD FML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRELVDRFML 480

Query: 481 LSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSG NNSY QSQVLTEA QADKVVAGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEANQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVHM 600
           GYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+QIL QGTRKHRRT+SNGSTTSSRS PLREEKL+RS+ QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNRSSAQRARSQ 660

Query: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQNS 780
           QFLRTPLKEIADDEA IDFLLDEVIVAASERCLDPIPLEPPILDKLIQ KLAKA+DQNS
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 779

BLAST of Sed0012026 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1330.1 bits (3441), Expect = 0.0e+00
Identity = 708/779 (90.89%), Postives = 735/779 (94.35%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           ME ++  MDEK KRMRDLLSSFYSPD S SGS   SSNRYASPLEAIN+TSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAE+KNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           EQLLEKILSVQSRSDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARLGKCIK EAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
           ADAVR+YTGAMPIFK YGDSSFQDCKRASEEAIA VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLS  L IGSS +GNVSESVYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 QEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTDV 360
           +EFAEA+RAYRVIFADSD+QL KLA DLVTKHF++ EQFIKKQICAAD L VFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQV+VKQ+V   FSRLLQ+ISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGL I+QRDSIVDWVQEGFQ+FF ALVD FM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSG N+SY QSQVL EATQA+KVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           RGYEYGPAF+PAEICRMFRAAGEKFL+LYINMRSQRISVLL KRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLSRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL+RSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EF+RLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKDQN 779
           MQFLRTPLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLI+ KLAKAKDQN
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQN 779

BLAST of Sed0012026 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 973.8 bits (2516), Expect = 8.3e-284
Identity = 527/785 (67.13%), Postives = 631/785 (80.38%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           M TE  PMDEKAKRMRDLLSSFY+PD S+S   TS S+  AS  + INSTSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSIS---TSGSSINAS-FDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAE+KNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTRNLLRKVQFIYDLPARLGKCIKAEAY 180
           +QLL+KI+SVQS+SDGVNTSL EKREHIEK HRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIATVLKKLQEKLFSDSESIQTRAEAAVL 240
            DAVR+YTGAMPI K YGD+SFQDC+RASEEAI  ++K LQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSRVLAIGSSMEGNVSESVYGAS-HEAS 300
           LKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S ++    S     S   + A  HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VQEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFDSTEQFIKKQICAADFLHVFGIIWTD 360
           V+ F+EA+RAYR IF DS+++L KLA  L   HF+  E +IKK++ AADFL +F I+W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSRLLQEISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV++KQFV   FS L Q+ISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIINQRDSIVDWVQEGFQEFFSALVDHFM 480
           ++ LEAS+KAVLQG+ ++  DFRQLL++++G+ I  +D I  W+Q+G Q+FF +L   F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSG  +S        E   +DK+ AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQRISVLLKKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL+KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPQGT-RKHRRTDSNGS--TTSSRSNPLREEKLSRSNTQR 660
           M+VD+FL ELE VG EVKQ+LPQGT RKH+RTDSNGS  TTSSRSN L  +K++RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLPLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL E+HLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRTPLKEIADDEAVIDFLLDEVIVAASERCLDPIPLEPPILDKLIQTKLAKAKD 780
           QLD+QFL+ PLKE  +DEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQ KLAK+K+
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 777

Query: 781 QNSAT 782
            N+ T
Sbjct: 781 HNNNT 777

BLAST of Sed0012026 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 959.9 bits (2480), Expect = 1.2e-279
Identity = 527/810 (65.06%), Postives = 631/810 (77.90%), Query Frame = 0

Query: 1   METEDVPMDEKAKRMRDLLSSFYSPDVSMSGSPTSSSNRYASPLEAINSTSFNPDQYMSI 60
           M TE  PMDEKAKRMRDLLSSFY+PD S+S   TS S+  AS  + INSTSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSIS---TSGSSINAS-FDNINSTSFDADQYMDL 60

Query: 61  L-------------------------VQKSNLEGLLQRHVEMAAEMKNLDTDLQMLVYEN 120
           +                         ++KSNLE LLQRHV+MAAE+KNLDTDLQMLVYEN
Sbjct: 61  MIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYEN 120

Query: 121 YNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLLEKREHIEKSHRTR 180
           YNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDGVNTSL EKREHIEK HRTR
Sbjct: 121 YNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTR 180

Query: 181 NLLRKVQFIYDLPARLGKCIKAEAYADAVRYYTGAMPIFKAYGDSSFQDCKRASEEAIAT 240
           NLLRKVQFIYDLPARL KCIK+EAY DAVR+YTGAMPI K YGD+SFQDC+RASEEAI  
Sbjct: 181 NLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEI 240

Query: 241 VLKKLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSR 300
           ++K LQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S 
Sbjct: 241 IIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEAST 300

Query: 301 VLAIGSSMEGNVSESVYGAS-HEASVQEFAEAVRAYRVIFADSDKQLSKLALDLVTKHFD 360
           ++    S     S   + A  HE +V+ F+EA+RAYR IF DS+++L KLA  L   HF+
Sbjct: 301 LVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFE 360

Query: 361 STEQFIKKQICAADFLHVFGIIWTDVLLLGEVLNDACLPDYSLKAAQVSVKQFVKSTFSR 420
             E +IKK++ AADFL +F I+W DV+L+ EVL +A L D S +AAQV++KQFV   FS 
Sbjct: 361 YMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSH 420

Query: 421 LLQEISDALTQVHTRKKEGVQEYSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLIIN 480
           L Q+ISD L +    +KE V+   L++ LEAS+KAVLQG+ ++  DFRQLL++++G+ I 
Sbjct: 421 LQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIK 480

Query: 481 QRDSIVDWVQEGFQEFFSALVDHFMLLSGNNNSYGQSQVLTEATQADKVVAGLVLVLAQI 540
            +D I  W+Q+G Q+FF +L   F++LSG  +S        E   +DK+ AGL+LVLAQ+
Sbjct: 481 MKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----IEGKSSDKIHAGLILVLAQL 540

Query: 541 SVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFLPAEICRMFRAAGEKFLNLYINMRSQ 600
           SVFIEQ  IPR+TEEIAASFSGG  + +E GPAF+P E+CR+F AA EK L  YI+ R+Q
Sbjct: 541 SVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQ 600

Query: 601 RISVLLKKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILPQGT-RKHRRTDSN 660
           ++SVLL+KRF+TPNWVKHKEPREVHM+VD+FL ELE VG EVKQ+LPQGT RKH+RTDSN
Sbjct: 601 KVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSN 660

Query: 661 GS--TTSSRSNPLREEKLSRSNTQRARSQLLESHLAKLFKQKIEIFTRVEFTQGSVVTTA 720
           GS  TTSSRSN L  +K++RSN+QRARSQL E+HLAKLFKQK+EIFT+VEFTQ SVVTT 
Sbjct: 661 GSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTT 720

Query: 721 VKLPLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAVIDFLLDEVIVAASER 780
           VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +DEA IDFLLDEVIVAASER
Sbjct: 721 VKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASER 780

Query: 781 CLDPIPLEPPILDKLIQTKLAKAKDQNSAT 782
           CLD IPLEPPILDKLIQ KLAK+K+ N+ T
Sbjct: 781 CLDVIPLEPPILDKLIQAKLAKSKEHNNNT 802