Sed0012020 (gene) Chayote v1

Overview
NameSed0012020
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG02: 51255232 .. 51256625 (+)
RNA-Seq ExpressionSed0012020
SyntenySed0012020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGATCATAAGATATAAAAACTTGTGGTTCTTCCATGGATTCCGATTTTCCTCCTCTTTTCCAATGCCCTATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTTCACCGGCGTCACCTACGAGCGAAAAAGCATCGAAAAATGGCTCTTTACGTACAACAACAAAACCTGCCCGGCGACCATGCAGACCCTCGCCGCCGGCGACGAAGACTTCGTCATCACCCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATCGATCATTCTTCGGCTCCGGCGATGGAGGAAGTCCGGTCGCTTCTTGCGACGGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGGTCGGTGATTGGAATGAGCGATGAGATGAAACTGGAATTTGTTGGATTGAATGGGGTTGGGATTGTTGTGAATGTTGTGGTTCAGATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCCTGTGAGGAGGCTTTGGGAGTGTTGTCTGAGTTTCCGATTTCGGATGAAGATAAAGTGTTCGAAAAAATGTCGAACAGAGAAGAGTTGATGAAATCCATCAGTATTGTTCTTCAAAGGGGAAGTGCAGAAGGGCGTTTTTATTCTGTGAAAATCCTTAGAAATTTTGCTAAGGACGCTGAATTCAATTGGGGCTCTGTCTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTCTCCACAAAGGCTAGCTCGTGTGCGTTGGATGTAATGATGGAGATTCTAGCCTCGTCCAAGAGGAGTCGAGTGAAGGCGATCGAAGCCGGTGCCGTGTGCGTTCTCATCGAGCTTTTGCCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCATAGTTTGTGTGAATGTGCAGAAGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCTAATGTGGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTTTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCACATGGACGGCGTCGGTGTTGGCGGCGGTGGCCGGAGTTCGACAAAGGAGAAGGTGATTAAGATTTTGAAGATGAATGGGAGTTCATGGAGGCGTAGTCCTTGTTTTCCTAGAGAGTTGAAGAATTATTTGAAGTTTGTAAATTATGAATGACATATTGTTCTTCCTACAGAAATCAATATAGAAAAAAAAAGAAAATAAAGTGTTGTCACATTTTTCCTTCATGTTTGATTTGGTTTTGGAAATATATAGTAATTGGTTAGGGTGATTTCT

mRNA sequence

GCAGATCATAAGATATAAAAACTTGTGGTTCTTCCATGGATTCCGATTTTCCTCCTCTTTTCCAATGCCCTATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTTCACCGGCGTCACCTACGAGCGAAAAAGCATCGAAAAATGGCTCTTTACGTACAACAACAAAACCTGCCCGGCGACCATGCAGACCCTCGCCGCCGGCGACGAAGACTTCGTCATCACCCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATCGATCATTCTTCGGCTCCGGCGATGGAGGAAGTCCGGTCGCTTCTTGCGACGGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGGTCGGTGATTGGAATGAGCGATGAGATGAAACTGGAATTTGTTGGATTGAATGGGGTTGGGATTGTTGTGAATGTTGTGGTTCAGATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCCTGTGAGGAGGCTTTGGGAGTGTTGTCTGAGTTTCCGATTTCGGATGAAGATAAAGTGTTCGAAAAAATGTCGAACAGAGAAGAGTTGATGAAATCCATCAGTATTGTTCTTCAAAGGGGAAGTGCAGAAGGGCGTTTTTATTCTGTGAAAATCCTTAGAAATTTTGCTAAGGACGCTGAATTCAATTGGGGCTCTGTCTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTCTCCACAAAGGCTAGCTCGTGTGCGTTGGATGTAATGATGGAGATTCTAGCCTCGTCCAAGAGGAGTCGAGTGAAGGCGATCGAAGCCGGTGCCGTGTGCGTTCTCATCGAGCTTTTGCCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCATAGTTTGTGTGAATGTGCAGAAGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCTAATGTGGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTTTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCACATGGACGGCGTCGGTGTTGGCGGCGGTGGCCGGAGTTCGACAAAGGAGAAGGTGATTAAGATTTTGAAGATGAATGGGAGTTCATGGAGGCGTAGTCCTTGTTTTCCTAGAGAGTTGAAGAATTATTTGAAGTTTGTAAATTATGAATGACATATTGTTCTTCCTACAGAAATCAATATAGAAAAAAAAAGAAAATAAAGTGTTGTCACATTTTTCCTTCATGTTTGATTTGGTTTTGGAAATATATAGTAATTGGTTAGGGTGATTTCT

Coding sequence (CDS)

ATGGATTCCGATTTTCCTCCTCTTTTCCAATGCCCTATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTTCACCGGCGTCACCTACGAGCGAAAAAGCATCGAAAAATGGCTCTTTACGTACAACAACAAAACCTGCCCGGCGACCATGCAGACCCTCGCCGCCGGCGACGAAGACTTCGTCATCACCCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATCGATCATTCTTCGGCTCCGGCGATGGAGGAAGTCCGGTCGCTTCTTGCGACGGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGGTCGGTGATTGGAATGAGCGATGAGATGAAACTGGAATTTGTTGGATTGAATGGGGTTGGGATTGTTGTGAATGTTGTGGTTCAGATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCCTGTGAGGAGGCTTTGGGAGTGTTGTCTGAGTTTCCGATTTCGGATGAAGATAAAGTGTTCGAAAAAATGTCGAACAGAGAAGAGTTGATGAAATCCATCAGTATTGTTCTTCAAAGGGGAAGTGCAGAAGGGCGTTTTTATTCTGTGAAAATCCTTAGAAATTTTGCTAAGGACGCTGAATTCAATTGGGGCTCTGTCTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTCTCCACAAAGGCTAGCTCGTGTGCGTTGGATGTAATGATGGAGATTCTAGCCTCGTCCAAGAGGAGTCGAGTGAAGGCGATCGAAGCCGGTGCCGTGTGCGTTCTCATCGAGCTTTTGCCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCATAGTTTGTGTGAATGTGCAGAAGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCTAATGTGGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTTTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCACATGGACGGCGTCGGTGTTGGCGGCGGTGGCCGGAGTTCGACAAAGGAGAAGGTGATTAAGATTTTGAAGATGAATGGGAGTTCATGGAGGCGTAGTCCTTGTTTTCCTAGAGAGTTGAAGAATTATTTGAAGTTTGTAAATTATGAATGA

Protein sequence

MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVITPNLTLKRLILAWKIDHSSAPAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSDEMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNREELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE
Homology
BLAST of Sed0012020 vs. NCBI nr
Match: XP_038902365.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 708.0 bits (1826), Expect = 4.8e-200
Identity = 360/413 (87.17%), Postives = 390/413 (94.43%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           M+SDFPPLF+CPISMELMEDPVT+ TGVTY+RKSIEKWLFTYN KTCPATMQTL+AGDED
Sbjct: 1   MESDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLSAGDED 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAP--AMEEVRSLLATVESSPFKVSSLRKLRSVIGMSD 120
           FVITPNLTLKRLILAWKI D SS+P  A+EEVRSLLATVESSPFKVSSLRKLRS+I MSD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSSSPATALEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120

Query: 121 EMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNR 180
            MK EF+  NG+GIVVNVV+QIL+DCSDFST E CEEALGVL +FPISDEDK+F++MS R
Sbjct: 121 GMKSEFIRSNGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISDEDKLFDEMSKR 180

Query: 181 EELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKS++IVLQRGSAEGRFY+VKILRN AK+  +NWGSV+EEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQGIDFFKPLLELVSDEF 240

Query: 241 STKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCEC 300
           +TKASSCALDVM+EILASSKRSRVKAIEAGAV +LIELLPESGRSKCERMLQIL SLCEC
Sbjct: 241 ATKASSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP+ERV+EEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPRERVVEEMMACGAVKK 360

Query: 361 LLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVN 411
           LLMILHMD VGVG GGRSSTKEKVIKILKM+G+SWRRSPCFP ELKNYLKFVN
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNSWRRSPCFPSELKNYLKFVN 413

BLAST of Sed0012020 vs. NCBI nr
Match: KAG7028702.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 701.4 bits (1809), Expect = 4.5e-198
Identity = 367/415 (88.43%), Postives = 387/415 (93.25%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNLTLKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK EFV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSCALDVMMEILA SKRSRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMD-GVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMD G G GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Sed0012020 vs. NCBI nr
Match: XP_022959873.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 366/415 (88.19%), Postives = 386/415 (93.01%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNLTLKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK EFV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSC LDVMMEILA SKRSRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMD-GVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMD G G GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Sed0012020 vs. NCBI nr
Match: KAG6597231.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 699.5 bits (1804), Expect = 1.7e-197
Identity = 366/415 (88.19%), Postives = 386/415 (93.01%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNLTLKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK EFV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSCALDVMMEILA SKRSRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRL LVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMD-GVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMD G G GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Sed0012020 vs. NCBI nr
Match: XP_022974967.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 693.7 bits (1789), Expect = 9.4e-196
Identity = 363/414 (87.68%), Postives = 383/414 (92.51%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNL LKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK  FV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSCALDVMMEILA SK SRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCALDVMMEILALSKSSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMDG   GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 409

BLAST of Sed0012020 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-51
Identity = 137/418 (32.78%), Postives = 218/418 (52.15%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + PP F CPIS+E+M+DPV + TG+TY+R SIEKWLF     +CP T Q +   D    +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD----L 70

Query: 64  TPNLTLKRLILAW----------KIDHSSAP-AMEEVRSLLA-TVESSPFKVSSLRKLRS 123
           TPN TL+RLI +W          +I     P    E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 VIGMSDEMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVF 183
           ++  +   K           + N+V       +D       +EAL +L  + +   + V 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLL--YHLETSETVL 190

Query: 184 EKMSNREE---LMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKP 243
           + + N ++   ++KS++ ++QRG  E R Y+  +L+N  + A+      L+    + F  
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTE 250

Query: 244 LLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPE----SGRSKC 303
           +++++ D  S KA+  A+ +++ I     R+R KA+EAG + V+IELL +    S R   
Sbjct: 251 VVQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGP 310

Query: 304 ERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKER 363
           E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     
Sbjct: 311 EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPA 370

Query: 364 VLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPREL 403
           +L EM+  G V KL ++L    V  GG     TKEK  ++LK++   W+ SPC P+ +
Sbjct: 371 LLHEMLQLGVVAKLCLVLQ---VSCGG----KTKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of Sed0012020 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.8e-48
Identity = 138/440 (31.36%), Postives = 224/440 (50.91%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + P  F CPIS+++M+DPV + TG+TY+R+SIEKWLF+    +CP T Q +   D    +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD----L 65

Query: 64  TPNLTLKRLILAW----------KIDHSSAP-AMEEVRSLLATVESSPF-KVSSLRKLRS 123
           TPN TL+RLI +W          +I     P    E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 VIGMSDEMKLEFVGLNGVGIVVNVVVQIL-------VDCSDFSTFEAC------------ 183
           ++  +   K           + N+V   +          S  +  + C            
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ----EEALGVLSEFPISDEDKVFEKMSNRE--ELMKSISIVLQRGSAEGRFYSVKILRNF 243
               +EAL VL     S E  +   ++N++   L+K+++ ++QRG  E R Y+  +L+  
Sbjct: 186 RSLMDEALSVLYHLDTS-ETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 245

Query: 244 AKDAEFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEA 303
            + A+     +LE    + F  +++++ D+ S KA+  A+ +++ I     R+R KA+E 
Sbjct: 246 LEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEG 305

Query: 304 GAVCVLIELLPE----SGRSKCERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVS 363
           G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS
Sbjct: 306 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 365

Query: 364 NVATKLGVKILLWISSFHPKERVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVI 403
            + ++  V++LL +  F     +L+EM+  G V KL ++L    V  G    + TKEK  
Sbjct: 366 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ---VSCG----NKTKEKAK 425

BLAST of Sed0012020 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.9e-34
Identity = 127/451 (28.16%), Postives = 213/451 (47.23%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L     D  +
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 68

Query: 64  TPNLTLKRLILAWKIDHSSAPAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE----- 123
           TPN  L+RLI  W +++ +   +      ++T    P K++ + +++++     E     
Sbjct: 69  TPNHMLRRLIQHWCVENETRGVVR-----ISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 128

Query: 124 ---MKLEFVGLNG------------VGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFP 183
               KLE + ++G              +++ VV     D       +  +E+L +L    
Sbjct: 129 ETLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 188

Query: 184 ISDEDKVFEKMSNREELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGI 243
           I   D     M N + +M+S++ VL +     + Y++ +LRN     E+    ++E    
Sbjct: 189 IPSNDAKTILMEN-DRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNP 248

Query: 244 DFFKPLLELVSDEFST-----------------------------------KASSCALDV 303
           + FK ++  + D  ++                                   +A + AL +
Sbjct: 249 EIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMI 308

Query: 304 MMEILASSKRSRVKAIEAGAVCVLIEL-LPESGRSK-CERMLQILHSLCECAEGRLALVE 363
           ++E  +S  R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ 
Sbjct: 309 LLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILA 368

Query: 364 HGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKLLMILHMDG 398
           H  GIA VTK++L VS  A    + IL  +S F P+  V+EEM+  G V+KL  +L MD 
Sbjct: 369 HRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD- 428

BLAST of Sed0012020 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.0e-32
Identity = 121/409 (29.58%), Postives = 200/409 (48.90%), Query Frame = 0

Query: 6   PPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVITP 65
           P  F+CPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLTLKRLILAW----------KIDHSSAPA--------MEEVRSLLATVESSPFKVSSLR 125
           N TL+RLI  W          +I     PA        + +  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSVIGMSDEMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDE 185
           +LR     SD+ ++     N   I++ ++       S+ ++ E   E+L +L   PI++ 
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPITEP 194

Query: 186 DKVFEKMSNREELMKSISIVLQRGSAEGRFYS---VKILRNFAKDAEFNWGSVLEEQGID 245
           ++ F  +S+    ++ ++ +L   S E R  +   ++I+    K A+   GS+   + + 
Sbjct: 195 NQ-FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLK-GSISNSESV- 254

Query: 246 FFKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIE-LLPESGRSK 305
            F+ +L+L+ +  S++ +       +  L S K +R  AI AGA  +LI+ L  +  R  
Sbjct: 255 -FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 314

Query: 306 CERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKE 365
            ER L  +  LC   EG  A  EH + +  + K IL VS+ AT+     LL + +   +E
Sbjct: 315 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEE 374

Query: 366 RVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSW 393
           R  EE    G V +LL+++  +            K+K  K+LK+   SW
Sbjct: 375 RWREEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

BLAST of Sed0012020 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.1e-28
Identity = 113/404 (27.97%), Postives = 195/404 (48.27%), Query Frame = 0

Query: 6   PPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVITP 65
           PP FQCPIS++LM+DPV I TG+TY+R SIE W+    NKTCP T   L   D+     P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQ----IP 91

Query: 66  NLTLKRLILAWKIDHSSAPAMEEVRSLLATVESSPFKVSSL-RKLRSVIGMSDEMKLEFV 125
           N T++++I  W ++  S P ++ + +    V   P +V  + RKL S     D  K   +
Sbjct: 92  NHTIRKMIQGWCVEKGS-PLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEKCGVI 151

Query: 126 -----------GLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSE--------FPIS 185
                        N   +  N V  +L DC  F  F   E+   +L+E        FPI 
Sbjct: 152 IEKIKKLGDESEKNRKCVNENSVGWVLCDC--FDKFSGDEKLTFMLNEILSLLTWMFPIG 211

Query: 186 DEDKVFEKMSNREELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDF 245
            E     K+++     + ++ +L+      R  +  I++      E    S   E G+  
Sbjct: 212 LEG--ISKLASATS-FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV-- 271

Query: 246 FKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCE 305
            + L++L+ D  S+ ++  +L  + +++        + +E G V + +E++ ++  S CE
Sbjct: 272 AEALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCE 331

Query: 306 RMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKIL--LWISSFHPKE 365
           + L +L ++CE   GR  + ++ + +  + KKI  VS +AT+  + ++  LW +      
Sbjct: 332 KALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---NT 391

Query: 366 RVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKM 388
             +E+ +  GA +K+L++L    VG G      TKEK  ++LKM
Sbjct: 392 VAVEDAVRLGAFQKVLLVLQ---VGYG----EETKEKATELLKM 410

BLAST of Sed0012020 vs. ExPASy TrEMBL
Match: A0A6J1H765 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 6.3e-198
Identity = 366/415 (88.19%), Postives = 386/415 (93.01%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNLTLKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK EFV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSC LDVMMEILA SKRSRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMD-GVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMD G G GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Sed0012020 vs. ExPASy TrEMBL
Match: A0A6J1IKT9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 PE=4 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.5e-196
Identity = 363/414 (87.68%), Postives = 383/414 (92.51%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF CPISMELMEDPVTI TGVTYERKSIEKWLFTYNNKTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60

Query: 61  FVITPNLTLKRLILAWKI-DHSSAPA-MEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE 120
           FVITPNL LKRLILAWKI D S++PA +EEVRSLLATVESSPFKVSSLRKLRS+I  SDE
Sbjct: 61  FVITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDE 120

Query: 121 MKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNRE 180
           MK  FV LNG+GIVVNVVVQIL+DCSDF TFEACEEA+GVLS+ PIS+EDK+F++MS RE
Sbjct: 121 MKSGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKRE 180

Query: 181 ELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEFS 240
           ELMKSI+IVLQRGSAEGRFY+VKILRN AK+  FNWGSVLEEQGIDFFKPLLELVSDEFS
Sbjct: 181 ELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFS 240

Query: 241 TKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCECA 300
           TKASSCALDVMMEILA SK SRVKAIEAGAVC+LIELLPESGRSKCERMLQIL SLCECA
Sbjct: 241 TKASSCALDVMMEILALSKSSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECA 300

Query: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKL 360
           EGRLALVEHGMGIAAVTKKILNVSNVATK+GVKILLWISSFHP ERV+EEMMACG VKKL
Sbjct: 301 EGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKL 360

Query: 361 LMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           +MILHMDG   GGGGRSSTKEK IKILKM+GSSWRRSPCFP ELKNYL FVN E
Sbjct: 361 MMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 409

BLAST of Sed0012020 vs. ExPASy TrEMBL
Match: A0A1S3AWJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.3e-194
Identity = 350/415 (84.34%), Postives = 383/415 (92.29%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF+CPISMELMEDPVT+ TGVTY+RKSIEKWLFTYN KTCPATMQTLAAG +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKIDHSS---APAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSD 120
           FVITPNLTLKRLILAWKI+ S+   A A+EEV SLLATVESSPFKVSSLRKLRS+I M+D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 121 EMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNR 180
            MK EFV L+G+GIVVNVV+QIL+DCSDFST E CEEALGVL +FPIS+EDK+F++M  R
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKSI++VLQRGSAEGRFY+VKILRN AK+  +NWGSV+EEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 STKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCEC 300
            TKASSCALDVM+ IL+SSKRSRVKAIEAGAV +LIELLPESGRSKCERMLQIL SLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWISSFHP+ERV++EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           LLMILHMD VGVG GGRSSTKEKVIKILKM+G+ WRRSPCFP ELKNYLKFVN E
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of Sed0012020 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.6e-194
Identity = 350/415 (84.34%), Postives = 383/415 (92.29%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF+CPISMELMEDPVT+ TGVTY+RKSIEKWLFTYN KTCPATMQTLAAG +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKIDHSS---APAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSD 120
           FVITPNLTLKRLILAWKI+ S+   A A+EEV SLLATVESSPFKVSSLRKLRS+I M+D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 EMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNR 180
            MK EFV L+G+GIVVNVV+QIL+DCSDFST E CEEALGVL +FPIS+EDK+F++M  R
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKSI++VLQRGSAEGRFY+VKILRN AK+  +NWGSV+EEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 STKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCEC 300
            TKASSCALDVM+ IL+SSKRSRVKAIEAGAV +LIELLPESGRSKCERMLQIL SLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWISSFHP+ERV++EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVNYE 413
           LLMILHMD VGVG GGRSSTKEKVIKILKM+G+ WRRSPCFP ELKNYLKFVN E
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of Sed0012020 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 9.5e-194
Identity = 349/413 (84.50%), Postives = 383/413 (92.74%), Query Frame = 0

Query: 1   MDSDFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDED 60
           MDSDFPPLF+CPISMELMEDPVTI TGVTYERKSIEKWLFTYN +TCPATMQT+A G+ D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLTLKRLILAWKIDHSS---APAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSD 120
           FV+TPNLTLKR+ILAWKI+ SS   A A+++V+SLLATVESSPFKVSSLRKLRS+I M D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 EMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVFEKMSNR 180
           E+K EF+ LNG+GIVVNVVVQIL+DCSDFST EACEEALGVLS+FPIS+ED++F++MS R
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192

Query: 181 EELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKS++IVLQRGSAEGRFY+VKILRN AK+  +NWG V+EEQGID FKPLLELVSDEF
Sbjct: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 STKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCERMLQILHSLCEC 300
            TK+SSCALDVM+EILASSKRSRVKAIEAGAV +LIELLPES RSKCERMLQIL SLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSNVATK+GVKIL WISSFHPKERV+EEMMACGAVKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPRELKNYLKFVN 411
           LLMILHM   GVGGGGRSSTKEKVIKILKM+GSSWRRSPCFP ELKNYLK VN
Sbjct: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVN 425

BLAST of Sed0012020 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 205.3 bits (521), Expect = 9.4e-53
Identity = 137/418 (32.78%), Postives = 218/418 (52.15%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + PP F CPIS+E+M+DPV + TG+TY+R SIEKWLF     +CP T Q +   D    +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD----L 70

Query: 64  TPNLTLKRLILAW----------KIDHSSAP-AMEEVRSLLA-TVESSPFKVSSLRKLRS 123
           TPN TL+RLI +W          +I     P    E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 VIGMSDEMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDEDKVF 183
           ++  +   K           + N+V       +D       +EAL +L  + +   + V 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLL--YHLETSETVL 190

Query: 184 EKMSNREE---LMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDFFKP 243
           + + N ++   ++KS++ ++QRG  E R Y+  +L+N  + A+      L+    + F  
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTE 250

Query: 244 LLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPE----SGRSKC 303
           +++++ D  S KA+  A+ +++ I     R+R KA+EAG + V+IELL +    S R   
Sbjct: 251 VVQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGP 310

Query: 304 ERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKER 363
           E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     
Sbjct: 311 EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPA 370

Query: 364 VLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSWRRSPCFPREL 403
           +L EM+  G V KL ++L    V  GG     TKEK  ++LK++   W+ SPC P+ +
Sbjct: 371 LLHEMLQLGVVAKLCLVLQ---VSCGG----KTKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of Sed0012020 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 193.0 bits (489), Expect = 4.9e-49
Identity = 138/440 (31.36%), Postives = 224/440 (50.91%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + P  F CPIS+++M+DPV + TG+TY+R+SIEKWLF+    +CP T Q +   D    +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD----L 65

Query: 64  TPNLTLKRLILAW----------KIDHSSAP-AMEEVRSLLATVESSPF-KVSSLRKLRS 123
           TPN TL+RLI +W          +I     P    E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 VIGMSDEMKLEFVGLNGVGIVVNVVVQIL-------VDCSDFSTFEAC------------ 183
           ++  +   K           + N+V   +          S  +  + C            
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ----EEALGVLSEFPISDEDKVFEKMSNRE--ELMKSISIVLQRGSAEGRFYSVKILRNF 243
               +EAL VL     S E  +   ++N++   L+K+++ ++QRG  E R Y+  +L+  
Sbjct: 186 RSLMDEALSVLYHLDTS-ETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 245

Query: 244 AKDAEFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEA 303
            + A+     +LE    + F  +++++ D+ S KA+  A+ +++ I     R+R KA+E 
Sbjct: 246 LEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEG 305

Query: 304 GAVCVLIELLPE----SGRSKCERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVS 363
           G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS
Sbjct: 306 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 365

Query: 364 NVATKLGVKILLWISSFHPKERVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVI 403
            + ++  V++LL +  F     +L+EM+  G V KL ++L    V  G    + TKEK  
Sbjct: 366 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ---VSCG----NKTKEKAK 425

BLAST of Sed0012020 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-35
Identity = 127/451 (28.16%), Postives = 213/451 (47.23%), Query Frame = 0

Query: 4   DFPPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L     D  +
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 82

Query: 64  TPNLTLKRLILAWKIDHSSAPAMEEVRSLLATVESSPFKVSSLRKLRSVIGMSDE----- 123
           TPN  L+RLI  W +++ +   +      ++T    P K++ + +++++     E     
Sbjct: 83  TPNHMLRRLIQHWCVENETRGVVR-----ISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 142

Query: 124 ---MKLEFVGLNG------------VGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFP 183
               KLE + ++G              +++ VV     D       +  +E+L +L    
Sbjct: 143 ETLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 202

Query: 184 ISDEDKVFEKMSNREELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGI 243
           I   D     M N + +M+S++ VL +     + Y++ +LRN     E+    ++E    
Sbjct: 203 IPSNDAKTILMEN-DRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNP 262

Query: 244 DFFKPLLELVSDEFST-----------------------------------KASSCALDV 303
           + FK ++  + D  ++                                   +A + AL +
Sbjct: 263 EIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMI 322

Query: 304 MMEILASSKRSRVKAIEAGAVCVLIEL-LPESGRSK-CERMLQILHSLCECAEGRLALVE 363
           ++E  +S  R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ 
Sbjct: 323 LLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILA 382

Query: 364 HGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKERVLEEMMACGAVKKLLMILHMDG 398
           H  GIA VTK++L VS  A    + IL  +S F P+  V+EEM+  G V+KL  +L MD 
Sbjct: 383 HRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD- 442

BLAST of Sed0012020 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 140.6 bits (353), Expect = 2.9e-33
Identity = 121/409 (29.58%), Postives = 200/409 (48.90%), Query Frame = 0

Query: 6   PPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVITP 65
           P  F+CPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLTLKRLILAW----------KIDHSSAPA--------MEEVRSLLATVESSPFKVSSLR 125
           N TL+RLI  W          +I     PA        + +  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSVIGMSDEMKLEFVGLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSEFPISDE 185
           +LR     SD+ ++     N   I++ ++       S+ ++ E   E+L +L   PI++ 
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPITEP 194

Query: 186 DKVFEKMSNREELMKSISIVLQRGSAEGRFYS---VKILRNFAKDAEFNWGSVLEEQGID 245
           ++ F  +S+    ++ ++ +L   S E R  +   ++I+    K A+   GS+   + + 
Sbjct: 195 NQ-FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLK-GSISNSESV- 254

Query: 246 FFKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIE-LLPESGRSK 305
            F+ +L+L+ +  S++ +       +  L S K +R  AI AGA  +LI+ L  +  R  
Sbjct: 255 -FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 314

Query: 306 CERMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKILLWISSFHPKE 365
            ER L  +  LC   EG  A  EH + +  + K IL VS+ AT+     LL + +   +E
Sbjct: 315 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEE 374

Query: 366 RVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKMNGSSW 393
           R  EE    G V +LL+++  +            K+K  K+LK+   SW
Sbjct: 375 RWREEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

BLAST of Sed0012020 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-29
Identity = 113/404 (27.97%), Postives = 195/404 (48.27%), Query Frame = 0

Query: 6   PPLFQCPISMELMEDPVTIFTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDEDFVITP 65
           PP FQCPIS++LM+DPV I TG+TY+R SIE W+    NKTCP T   L   D+     P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQ----IP 91

Query: 66  NLTLKRLILAWKIDHSSAPAMEEVRSLLATVESSPFKVSSL-RKLRSVIGMSDEMKLEFV 125
           N T++++I  W ++  S P ++ + +    V   P +V  + RKL S     D  K   +
Sbjct: 92  NHTIRKMIQGWCVEKGS-PLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEKCGVI 151

Query: 126 -----------GLNGVGIVVNVVVQILVDCSDFSTFEACEEALGVLSE--------FPIS 185
                        N   +  N V  +L DC  F  F   E+   +L+E        FPI 
Sbjct: 152 IEKIKKLGDESEKNRKCVNENSVGWVLCDC--FDKFSGDEKLTFMLNEILSLLTWMFPIG 211

Query: 186 DEDKVFEKMSNREELMKSISIVLQRGSAEGRFYSVKILRNFAKDAEFNWGSVLEEQGIDF 245
            E     K+++     + ++ +L+      R  +  I++      E    S   E G+  
Sbjct: 212 LEG--ISKLASATS-FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV-- 271

Query: 246 FKPLLELVSDEFSTKASSCALDVMMEILASSKRSRVKAIEAGAVCVLIELLPESGRSKCE 305
            + L++L+ D  S+ ++  +L  + +++        + +E G V + +E++ ++  S CE
Sbjct: 272 AEALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCE 331

Query: 306 RMLQILHSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKLGVKIL--LWISSFHPKE 365
           + L +L ++CE   GR  + ++ + +  + KKI  VS +AT+  + ++  LW +      
Sbjct: 332 KALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---NT 391

Query: 366 RVLEEMMACGAVKKLLMILHMDGVGVGGGGRSSTKEKVIKILKM 388
             +E+ +  GA +K+L++L    VG G      TKEK  ++LKM
Sbjct: 392 VAVEDAVRLGAFQKVLLVLQ---VGYG----EETKEKATELLKM 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902365.14.8e-20087.17E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
KAG7028702.14.5e-19888.43E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022959873.11.3e-19788.19E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata][more]
KAG6597231.11.7e-19788.19E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022974967.19.4e-19687.68E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubi... [more]
Match NameE-valueIdentityDescription
Q84TG31.3e-5132.78E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC66.8e-4831.36E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF151.9e-3428.16E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT794.0e-3229.58U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q5PNY62.1e-2827.97U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1H7656.3e-19888.19RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728... [more]
A0A6J1IKT94.5e-19687.68RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 P... [more]
A0A1S3AWJ23.3e-19484.34RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 ... [more]
A0A5D3D2G25.6e-19484.34RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1IYW39.5e-19484.50RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P... [more]
Match NameE-valueIdentityDescription
AT2G35930.19.4e-5332.78plant U-box 23 [more]
AT3G52450.14.9e-4931.36plant U-box 22 [more]
AT3G11840.11.4e-3528.16plant U-box 24 [more]
AT3G19380.12.9e-3329.58plant U-box 25 [more]
AT5G37490.11.5e-2927.97ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 8..76
e-value: 1.1E-18
score: 78.1
IPR003613U box domainPFAMPF04564U-boxcoord: 6..81
e-value: 9.1E-14
score: 51.4
IPR003613U box domainPROSITEPS51698U_BOXcoord: 4..83
score: 21.390669
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..91
e-value: 2.5E-21
score: 77.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 129..387
e-value: 1.4E-13
score: 52.1
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 5..410
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 5..410
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 9..52
e-value: 3.15108E-20
score: 81.1259
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..81
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 85..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012020.1Sed0012020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity