Sed0011294 (gene) Chayote v1

Overview
NameSed0011294
Typegene
OrganismSechium edule (Chayote v1)
DescriptionL-ascorbate oxidase homolog
LocationLG04: 46649986 .. 46654455 (-)
RNA-Seq ExpressionSed0011294
SyntenySed0011294
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCACTTTAAATACACCATTTGGTTCTTGAGCAGTTACCCACTCACTTTTTAAAACCTGAAAGAAACTCCATTTATAACTCAAACCCCTTTCTCTTTATATCTCTCTCTTCTTAACACATTTTTTCAGAACCCACAAAATGGTGGTCAAAATGGCAAAAAGTTCAGCTAAGTTATGCTGTTTTCATGTCGCTCTGCTTTTCGGAGTCATCGGCATTGCCTTCGCCGATAGCCCGTATCGATTCTTCGATTGGAACGTCACCTACGGCGATATCTATCCCCTCGGTGTTCGCCAACAGGTGAGATTCAATTTACTATAATCTATATCGATTACGTTCGGGTGAAAGATTTGAACTTTTAACTTAATGGAATCCTATTTTTTTTATGTTTGTAGGGAATTTTGATAAATGGGCAATTTCCGGGACCGGATATTTATGCTGTGACAAATGATAATATTTATATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTCTCTTGGTTCGTATTTTCTCTCTCTTTCTTTATAATGTGGAATAGTTTTGTGATGAAGAAAAGAATTTAGAATCAAGATTGATTGAATTTTCAGTTATCAGAGTGTTTTTTTAATACTTTATAAGAGTTCATATTTTCTTTCTTTTTTCTTGTTTTTAAAGATGTTTTAAATATTTTAGTGAATATTTCTTATAGAAAGTATTAGCTTCGGGAAAATATTTTATTTTGCAAACGCGTGGGAATAATTTGAAAGTATTCTTTTGGGAATGTGTGAATGAATAAAAGAAATTAGGGGAGAATTAGCTTAATCTTTAAAAATAGAAATGTTGCATTTTATAAAATAAGGAAGCATAAATTCAAACCTATAGATTCTAAAGAAAATAATAATAATAAATTCTAAAGAAAAATGAAACATTAACCTTTTTTAGGAGATAATGTCAACCTTTTTAATTCAACTGAAAACATGAATCTTGTGATGTTTTAGTCCATTTTATGAATGTTTAACTCAAAACTTAAATTTGACAATCGTTAAGTTACTTGGTCTTTGTCGGATAGTTATTTATAATTTATTTTTTAAAATTATAAATATAAATATAAATAATGCTTATAAACTAAATGAAAAAGAATAGAATTGAAATATATATATATATATTTTAAAGTTCATATTAGGGAAGGAGGACAATAATTTTCAGAATTGATCCTCTAACCAGTAAAATAAGTATCGTTGAATATGAAAGAAATGACCACAATAGTTCAAATAATACAAAGGTAAGTTTATAATAAAAATGTATGAAGTTTGAAATCCCTACCTCAATCACTATTGAACTCTAAATTTTTTTTTTTTAAAAAGTTAGAATTAAAATTTTGACTTTATTTTATTTCAATATTGAGTTGGTGACTCAAATGATATGATGTTTTGGTGATTAAAAGATCACTAGTTCTAAATATCTTGTTATAAGTTGAATCTAGAGAAATCTTACTCCATAAATACTTCCAACAAAATAATTATAAATGCAGATAGAATCTAAATTATTTAATTATATTATTAATACGTTTTAATACTATATTATAAATACTTCTTTTTTTGAAATAGAGTATTGTAAATATAGTATGTGATGGCATATTTGCTGATTTACTTAAAAAGAAAAAGAAAAAGTAGTTTTTGGTAGTTGAATCTTTCTTCATTGGACCTTAGGCCCATTAACTTTGGGCCTACCTGTTGGGCCCATAAATACTAACCGTTTTTTGTTTTTGTTAATTAAATTCAGGAACGGAATTCAACAGAGGAAAAACTCGTACCAGGACGGAGTGTACGGAACGACATGTCCAATTCCGGCGGGGAAGAACTTTACCTACAGATTTCAGGTGAAGGATCAGATCGGAAGTTTCTTCTACTTCCCTTCTCTTCAATTCCACAAAGCCGCCGGCGGATTCGGAAGCATCAGAGTTCTCAGCCGCCCCCTCATTCCGGTCCCCTTCCCGGAACCCGCCGCCGATTACACCCTCCTCGTCGGCGACTGGTACACCGCCAATCACACGGTAACCCAACCATTCCACTTTCCGCACTCTTAAATTATGCACAAGTTTGAAATCGATTTGATTGAGAATCGGAGATTTAGGGTTTTGGCGTTTGTTTCGTTGTTTTTTGGATTTAGACCTTGAAGAGTATCTTGGATCGTGGTCATAGGCTTCCTCTCCCTAATGGAATTGTTATCAATGGCCGTGGTCCCAATGCTACCACTTTCACCGTTGAACAGGGTAATAATTTCAGATTTGTTAATGTTTTAGGATGTGTTTTGTGGAATGGGATTATTTGAAGTGAGATTTTATGGGATTGTTGTTGTTGTTGCAGGTAAGACTTATAGGTTTAGAATATCGAATGTGGGGCTTGAGCATTCTGTTAATTTTAGGGTTCAAGATCATGAGATGATGCTCGTTGAAGTCGAGGGAACTCACACAATGCAGAATACCTATTCTTCGCTTGATGTTCATGTGGGTCAGTCTTATTCAGTGCTCATCACTGCGAATCAGCCGTCTCGTGATTTCTATATCGCTGTGTCGACTCGATTTGCCTCCAAGATCCTCACCTCCACTGCTATTCTTCACTATAGCGACTCCACCACCTCTGTGGCTGGTCCGATTCCAGGTGGACCGACGAATGAAATCGATTGGTCTCTTAACCAGGCTCGCTCCATTAGGTACTGACCATTGAATCTCTTCTCTCTTTGCTTGTTTTTTGAGAAAGAATTGATCATTGCTCAAGAATTAGAGGTTCTTACGCTTCTAATGTGCATTTATGTCATAACTTCACTTCCTTGGCCCTCTCATTAGTAGAGGCTTAAGGTCTCTAAGGTCTGCATCCTTGAAAGTCTTGAATTTGAAATCCACGAACGAGGTTAATATTAAATTTTTTTATTGTGAATGCCTCTGATTATATAGTGGAGCGAAGCTCTAACTTCCAATTATCTAAAAAGAAAAATCATATCATCACTTCAAAATTGTATGCATACTGGTCTGGAAGATTGATCATCTGTATACTGATGCTTCCATCGTTGTGTTCTCTGGCTTTAGGACGAATCTCACTGCGAGTGGACCAAGACCAAATCCACAGGGCTCGTATCACTATGGTTTGATAAATATTTCGAGGACCATTACGCTGGAGAGTTCTGCAGCTCTTGTTGATGGCAAGCAAAGATATGCTGTTAACAGTGTGTCGTTTATTCCTGCCGATACTCCTCTTAAGCTTGCAGATTACTTCAACATTGATGGTGTTTTTCACATTGGAAGTATCCCGGATTCTCCTTCTGGCAAGAACATGTACCTTGATACCTCCGTCTTGGGTACCGACTATAGAGCCTTTATTGAGATTGTTTTCCAGAACCGCGAGAACATTGTACAGAGTTGGCACATTGATGGATACGCCTTCTGGGTTGTCGGGTAAGAATCGCTTTGCTATTCAATCAACCCAACATCAATTGTATATTGTTATCAAGTTCATATTCATTGTTGTGTTGTTGCTAAATTAATCATTGGACACTAATTTATGTTGCAGAATGGATGGAGGAGTTTGGACTCCTGCTAGCAGAGAACAATACAATCTCAGAGATGCAGTTTCACGCTATACCACTCAGGTAAACCATCCCCCTCTTTCTAGATGTCAAAGTTCTAAATGTATGGAATCACATCCGTTGAGTTCGATATAAAATCTGAATCGAACCGTAAACTATGTATTGTATGTAGGTGTACCCGAAGTCATGGACTGCCATTTATGTTTCACTTGACAATGTTGGAATGTGGAACATAAGGTCAGAGTTTTGGGCAAGACAGTACCTTGGGCAACAGTTTTATCTCAGAGTTTACACACCAGTTAAGTCATTCAGGGATGAGTACAATATTCCAGATAATGCTCTACTTTGTGGTAGGGTTGCCAACCAAAGTACCATATCTCCTCCATAAATAACTGCTCTCAAGATTATACTTCACATAATTCTTTCAATTGAGCTTGCAAAAATCCCATTTGCACCAAATCTCCAGTATTTTTCTTAGAACATCTCATCAGCAATTTCTTCATCTTCTTGTACTTGCTTTCTTCCTCTGGGTTGGTGTTCATTCAAATTAACAAGCAATTATGTTGTAGAAAGAGAAAGGAGAGGAATAACTTGTTTACAAAGTTATACAATAAATTGATCTTGGAAAAATTTCAGGCATTTGTGATCCTCTGTTTCTTCTTCGTTTGTAATTTTATTAGAATGTGAAAGAGAAAGAAAGAGGAAAAAGGAAATGTAATAGAGAGGAAGATTGCACGTTTAGTAGTTTATTTTTCTTGAATTTCTGTGAATTATTGTACCCAATAAGAACAGTGCTCGATATTCTTTAATGATTATTTATTTGTTATGACGGCCGGTTTCTGTTTTGCTACCAGTCCACAAAAAAAGAGGGAACTAGAATAATATTGTTTACATTTCAATACCCCA

mRNA sequence

TCCACTTTAAATACACCATTTGGTTCTTGAGCAGTTACCCACTCACTTTTTAAAACCTGAAAGAAACTCCATTTATAACTCAAACCCCTTTCTCTTTATATCTCTCTCTTCTTAACACATTTTTTCAGAACCCACAAAATGGTGGTCAAAATGGCAAAAAGTTCAGCTAAGTTATGCTGTTTTCATGTCGCTCTGCTTTTCGGAGTCATCGGCATTGCCTTCGCCGATAGCCCGTATCGATTCTTCGATTGGAACGTCACCTACGGCGATATCTATCCCCTCGGTGTTCGCCAACAGGGAATTTTGATAAATGGGCAATTTCCGGGACCGGATATTTATGCTGTGACAAATGATAATATTTATATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTCTCTTGGAACGGAATTCAACAGAGGAAAAACTCGTACCAGGACGGAGTGTACGGAACGACATGTCCAATTCCGGCGGGGAAGAACTTTACCTACAGATTTCAGGTGAAGGATCAGATCGGAAGTTTCTTCTACTTCCCTTCTCTTCAATTCCACAAAGCCGCCGGCGGATTCGGAAGCATCAGAGTTCTCAGCCGCCCCCTCATTCCGGTCCCCTTCCCGGAACCCGCCGCCGATTACACCCTCCTCGTCGGCGACTGGTACACCGCCAATCACACGACCTTGAAGAGTATCTTGGATCGTGGTCATAGGCTTCCTCTCCCTAATGGAATTGTTATCAATGGCCGTGGTCCCAATGCTACCACTTTCACCGTTGAACAGGGTAAGACTTATAGGTTTAGAATATCGAATGTGGGGCTTGAGCATTCTGTTAATTTTAGGGTTCAAGATCATGAGATGATGCTCGTTGAAGTCGAGGGAACTCACACAATGCAGAATACCTATTCTTCGCTTGATGTTCATGTGGGTCAGTCTTATTCAGTGCTCATCACTGCGAATCAGCCGTCTCGTGATTTCTATATCGCTGTGTCGACTCGATTTGCCTCCAAGATCCTCACCTCCACTGCTATTCTTCACTATAGCGACTCCACCACCTCTGTGGCTGGTCCGATTCCAGGTGGACCGACGAATGAAATCGATTGGTCTCTTAACCAGGCTCGCTCCATTAGGACGAATCTCACTGCGAGTGGACCAAGACCAAATCCACAGGGCTCGTATCACTATGGTTTGATAAATATTTCGAGGACCATTACGCTGGAGAGTTCTGCAGCTCTTGTTGATGGCAAGCAAAGATATGCTGTTAACAGTGTGTCGTTTATTCCTGCCGATACTCCTCTTAAGCTTGCAGATTACTTCAACATTGATGGTGTTTTTCACATTGGAAGTATCCCGGATTCTCCTTCTGGCAAGAACATGTACCTTGATACCTCCGTCTTGGGTACCGACTATAGAGCCTTTATTGAGATTGTTTTCCAGAACCGCGAGAACATTGTACAGAGTTGGCACATTGATGGATACGCCTTCTGGGTTGTCGGAATGGATGGAGGAGTTTGGACTCCTGCTAGCAGAGAACAATACAATCTCAGAGATGCAGTTTCACGCTATACCACTCAGGTGTACCCGAAGTCATGGACTGCCATTTATGTTTCACTTGACAATGTTGGAATGTGGAACATAAGGTCAGAGTTTTGGGCAAGACAGTACCTTGGGCAACAGTTTTATCTCAGAGTTTACACACCAGTTAAGTCATTCAGGGATGAGTACAATATTCCAGATAATGCTCTACTTTGTGGTAGGGTTGCCAACCAAAGTACCATATCTCCTCCATAAATAACTGCTCTCAAGATTATACTTCACATAATTCTTTCAATTGAGCTTGCAAAAATCCCATTTGCACCAAATCTCCAGTATTTTTCTTAGAACATCTCATCAGCAATTTCTTCATCTTCTTGTACTTGCTTTCTTCCTCTGGGTTGGTGTTCATTCAAATTAACAAGCAATTATGTTGTAGAAAGAGAAAGGAGAGGAATAACTTGTTTACAAAGTTATACAATAAATTGATCTTGGAAAAATTTCAGGCATTTGTGATCCTCTGTTTCTTCTTCGTTTGTAATTTTATTAGAATGTGAAAGAGAAAGAAAGAGGAAAAAGGAAATGTAATAGAGAGGAAGATTGCACGTTTAGTAGTTTATTTTTCTTGAATTTCTGTGAATTATTGTACCCAATAAGAACAGTGCTCGATATTCTTTAATGATTATTTATTTGTTATGACGGCCGGTTTCTGTTTTGCTACCAGTCCACAAAAAAAGAGGGAACTAGAATAATATTGTTTACATTTCAATACCCCA

Coding sequence (CDS)

ATGGTGGTCAAAATGGCAAAAAGTTCAGCTAAGTTATGCTGTTTTCATGTCGCTCTGCTTTTCGGAGTCATCGGCATTGCCTTCGCCGATAGCCCGTATCGATTCTTCGATTGGAACGTCACCTACGGCGATATCTATCCCCTCGGTGTTCGCCAACAGGGAATTTTGATAAATGGGCAATTTCCGGGACCGGATATTTATGCTGTGACAAATGATAATATTTATATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTCTCTTGGAACGGAATTCAACAGAGGAAAAACTCGTACCAGGACGGAGTGTACGGAACGACATGTCCAATTCCGGCGGGGAAGAACTTTACCTACAGATTTCAGGTGAAGGATCAGATCGGAAGTTTCTTCTACTTCCCTTCTCTTCAATTCCACAAAGCCGCCGGCGGATTCGGAAGCATCAGAGTTCTCAGCCGCCCCCTCATTCCGGTCCCCTTCCCGGAACCCGCCGCCGATTACACCCTCCTCGTCGGCGACTGGTACACCGCCAATCACACGACCTTGAAGAGTATCTTGGATCGTGGTCATAGGCTTCCTCTCCCTAATGGAATTGTTATCAATGGCCGTGGTCCCAATGCTACCACTTTCACCGTTGAACAGGGTAAGACTTATAGGTTTAGAATATCGAATGTGGGGCTTGAGCATTCTGTTAATTTTAGGGTTCAAGATCATGAGATGATGCTCGTTGAAGTCGAGGGAACTCACACAATGCAGAATACCTATTCTTCGCTTGATGTTCATGTGGGTCAGTCTTATTCAGTGCTCATCACTGCGAATCAGCCGTCTCGTGATTTCTATATCGCTGTGTCGACTCGATTTGCCTCCAAGATCCTCACCTCCACTGCTATTCTTCACTATAGCGACTCCACCACCTCTGTGGCTGGTCCGATTCCAGGTGGACCGACGAATGAAATCGATTGGTCTCTTAACCAGGCTCGCTCCATTAGGACGAATCTCACTGCGAGTGGACCAAGACCAAATCCACAGGGCTCGTATCACTATGGTTTGATAAATATTTCGAGGACCATTACGCTGGAGAGTTCTGCAGCTCTTGTTGATGGCAAGCAAAGATATGCTGTTAACAGTGTGTCGTTTATTCCTGCCGATACTCCTCTTAAGCTTGCAGATTACTTCAACATTGATGGTGTTTTTCACATTGGAAGTATCCCGGATTCTCCTTCTGGCAAGAACATGTACCTTGATACCTCCGTCTTGGGTACCGACTATAGAGCCTTTATTGAGATTGTTTTCCAGAACCGCGAGAACATTGTACAGAGTTGGCACATTGATGGATACGCCTTCTGGGTTGTCGGAATGGATGGAGGAGTTTGGACTCCTGCTAGCAGAGAACAATACAATCTCAGAGATGCAGTTTCACGCTATACCACTCAGGTGTACCCGAAGTCATGGACTGCCATTTATGTTTCACTTGACAATGTTGGAATGTGGAACATAAGGTCAGAGTTTTGGGCAAGACAGTACCTTGGGCAACAGTTTTATCTCAGAGTTTACACACCAGTTAAGTCATTCAGGGATGAGTACAATATTCCAGATAATGCTCTACTTTGTGGTAGGGTTGCCAACCAAAGTACCATATCTCCTCCATAA

Protein sequence

MVVKMAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQSTISPP
Homology
BLAST of Sed0011294 vs. NCBI nr
Match: XP_022969054.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 492/544 (90.44%), Postives = 515/544 (94.67%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 64
           MA   A+L CF+VAL  G +GIA ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP
Sbjct: 1   MAPKPAELWCFYVALALGFMGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 60

Query: 65  DIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVK 124
           +IYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP GKNFTYR QVK
Sbjct: 61  EIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVK 120

Query: 125 DQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKS 184
           DQIG++FYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANH+ LK+
Sbjct: 121 DQIGTYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKT 180

Query: 185 ILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVE 244
           ILD GHRLP PNGIVINGRGP ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM LVE
Sbjct: 181 ILDGGHRLPNPNGIVINGRGPQATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVE 240

Query: 245 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDST 304
           VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKIL STAILHYSDST
Sbjct: 241 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILNSTAILHYSDST 300

Query: 305 TSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAA 364
           TSV+GPIPGGPTNEIDWSLNQARS+RTNLTASGPRPNPQGSYHYGLINISRTI LESSAA
Sbjct: 301 TSVSGPIPGGPTNEIDWSLNQARSVRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAA 360

Query: 365 LVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYR 424
           LVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IGSIPD PSGK MYLDTSV+G DYR
Sbjct: 361 LVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGSIPDFPSGKKMYLDTSVMGADYR 420

Query: 425 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 484
           AFIEIVFQN ENIVQSWH+DGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYPKSW
Sbjct: 421 AFIEIVFQNHENIVQSWHLDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPKSW 480

Query: 485 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQS 544
           TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKS RDEYN+PDNALLCG+ A +S
Sbjct: 481 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSLRDEYNVPDNALLCGKAAGRS 540

Query: 545 TISP 549
           T SP
Sbjct: 541 TASP 544

BLAST of Sed0011294 vs. NCBI nr
Match: XP_022952372.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6572144.1 hypothetical protein SDJN03_28872, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011793.1 hypothetical protein SDJN02_26699 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1033.5 bits (2671), Expect = 6.7e-298
Identity = 489/544 (89.89%), Postives = 514/544 (94.49%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 64
           MA   A+L CF+VA+  G IGIA ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP
Sbjct: 1   MAPKPAQLWCFYVAMALGFIGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 60

Query: 65  DIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVK 124
           +IYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP GKNFTYR QVK
Sbjct: 61  EIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVK 120

Query: 125 DQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKS 184
           DQIG++FYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANH+ LK 
Sbjct: 121 DQIGTYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKI 180

Query: 185 ILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVE 244
           ILD GHRLP PNGIVINGRGP ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM LVE
Sbjct: 181 ILDGGHRLPNPNGIVINGRGPLATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVE 240

Query: 245 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDST 304
           VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKIL STAILHYSDST
Sbjct: 241 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILNSTAILHYSDST 300

Query: 305 TSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAA 364
           TSV+GPIPGGPTNEIDWSLNQARS+RTNLTASGPRPNPQGSYHYGLINISRTI LESSAA
Sbjct: 301 TSVSGPIPGGPTNEIDWSLNQARSVRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAA 360

Query: 365 LVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYR 424
           LVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IG+IPD PSGK MYLDTSV+G DYR
Sbjct: 361 LVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGTIPDFPSGKKMYLDTSVMGADYR 420

Query: 425 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 484
           AFIEIVFQN ENIVQSWH+DGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYP+SW
Sbjct: 421 AFIEIVFQNHENIVQSWHLDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPRSW 480

Query: 485 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQS 544
           TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKS RDEYN+PDNALLCG+ A +S
Sbjct: 481 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSLRDEYNVPDNALLCGKAAGRS 540

Query: 545 TISP 549
           T +P
Sbjct: 541 TATP 544

BLAST of Sed0011294 vs. NCBI nr
Match: XP_023554680.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.1e-296
Identity = 488/544 (89.71%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 64
           MA   A+L CF+VA+  G IGIA ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP
Sbjct: 1   MAPKPAQLWCFYVAMALGFIGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 60

Query: 65  DIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVK 124
           +IYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPI  GKNFTYR QVK
Sbjct: 61  EIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPILPGKNFTYRIQVK 120

Query: 125 DQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKS 184
           DQIG++FYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANH+ LK 
Sbjct: 121 DQIGTYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKI 180

Query: 185 ILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVE 244
           ILD GHRLP PNGIVINGRGP ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM LVE
Sbjct: 181 ILDGGHRLPNPNGIVINGRGPLATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVE 240

Query: 245 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDST 304
           VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKIL STAILHYSDST
Sbjct: 241 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILNSTAILHYSDST 300

Query: 305 TSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAA 364
           TSV+GPIPGGPTNEIDWSLNQARS+RTNLTASGPRPNPQGSYHYGLINISRTI LESSAA
Sbjct: 301 TSVSGPIPGGPTNEIDWSLNQARSVRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAA 360

Query: 365 LVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYR 424
           LVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IGSIPD PSGK  YLDTSV+G DYR
Sbjct: 361 LVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGSIPDFPSGKKTYLDTSVMGADYR 420

Query: 425 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 484
           AFIEIVFQN ENIVQSWH+DGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYP+SW
Sbjct: 421 AFIEIVFQNHENIVQSWHLDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPRSW 480

Query: 485 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQS 544
           TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKS RDEYN+PDNALLCG+ A +S
Sbjct: 481 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSLRDEYNVPDNALLCGKAAGRS 540

Query: 545 TISP 549
           T +P
Sbjct: 541 TATP 544

BLAST of Sed0011294 vs. NCBI nr
Match: XP_038888634.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.7e-296
Identity = 489/546 (89.56%), Postives = 520/546 (95.24%), Query Frame = 0

Query: 5   MAKSSAKLC-CFHVALLFGV-IGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFP 64
           MA+ S  L   F+VAL+ G+ + +AFADSPYRFFDWNVTYGDIYPLG+RQ+GILINGQFP
Sbjct: 1   MAQRSPNLWGLFNVALVLGLFVNLAFADSPYRFFDWNVTYGDIYPLGIRQRGILINGQFP 60

Query: 65  GPDIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQ 124
           GPDIYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP G+NFTYR Q
Sbjct: 61  GPDIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRIQ 120

Query: 125 VKDQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTL 184
           VKDQIGSFFYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANHTTL
Sbjct: 121 VKDQIGSFFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTTL 180

Query: 185 KSILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMML 244
           K+ILDRGHRLPLPNGIVINGRGPNATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM L
Sbjct: 181 KTILDRGHRLPLPNGIVINGRGPNATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMKL 240

Query: 245 VEVEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSD 304
           VEVEGTHTMQNTYSSLDVHVGQSYSVLITA+QPSRDFYIAV+TRFA ++LTSTA+LHYSD
Sbjct: 241 VEVEGTHTMQNTYSSLDVHVGQSYSVLITADQPSRDFYIAVTTRFAPRVLTSTAVLHYSD 300

Query: 305 STTSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESS 364
           STTSVAGPIPGGPT EI+WS+NQARSIRTNLTASGPRPNPQGSYHYGLIN+SRTI LESS
Sbjct: 301 STTSVAGPIPGGPT-EIEWSINQARSIRTNLTASGPRPNPQGSYHYGLINVSRTIKLESS 360

Query: 365 AALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTD 424
           AALVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IGSIPD PSGK MYLDTSV+G D
Sbjct: 361 AALVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGSIPDFPSGKKMYLDTSVMGAD 420

Query: 425 YRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPK 484
           YRAFIEIVFQN ENIVQSWHIDGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYPK
Sbjct: 421 YRAFIEIVFQNHENIVQSWHIDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPK 480

Query: 485 SWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVAN 544
           SWTAIY+SLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYN+PDNALLCG+ A 
Sbjct: 481 SWTAIYMSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNVPDNALLCGKAAG 540

Query: 545 QSTISP 549
           +ST SP
Sbjct: 541 RSTASP 545

BLAST of Sed0011294 vs. NCBI nr
Match: XP_022135823.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1023.1 bits (2644), Expect = 9.0e-295
Identity = 489/548 (89.23%), Postives = 512/548 (93.43%), Query Frame = 0

Query: 1   MVVKMAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQ 60
           MV  +A+ + KL CF V  +  +I IAF+DSPYRFFDWNVTYGDIYPLGVRQQGILINGQ
Sbjct: 1   MVEIVAQRTPKLWCFIVFWMSVMINIAFSDSPYRFFDWNVTYGDIYPLGVRQQGILINGQ 60

Query: 61  FPGPDIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYR 120
           FPGPDIYAVTNDN+YINVHNSLPEPFLLSW+G+QQRKNSYQDGVYGTTCPIP GKNFTYR
Sbjct: 61  FPGPDIYAVTNDNLYINVHNSLPEPFLLSWSGVQQRKNSYQDGVYGTTCPIPPGKNFTYR 120

Query: 121 FQVKDQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHT 180
            QVKDQIGSFFYFPSL  HKAAGGFGSIRVLSRPLIPVPFP+PA D+TLL+GDWYT NH 
Sbjct: 121 IQVKDQIGSFFYFPSLALHKAAGGFGSIRVLSRPLIPVPFPDPAGDFTLLIGDWYTTNHK 180

Query: 181 TLKSILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEM 240
           TL++ILDRGHRLP PNGIVINGRGPNATTFTVE+GKTYRFRISNVGLEHS+NFRVQDHEM
Sbjct: 181 TLQTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLEHSLNFRVQDHEM 240

Query: 241 MLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHY 300
            LVEVEGTHTMQNTYSSLDVHVGQSYSVLITA+QPSRDFYIAVSTRFASKILTSTAILHY
Sbjct: 241 KLVEVEGTHTMQNTYSSLDVHVGQSYSVLITADQPSRDFYIAVSTRFASKILTSTAILHY 300

Query: 301 SDSTTSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLE 360
           SDSTTSVAGPIPGGPTN+IDWSLNQARSIRTNLTASGPRPNPQGSYHYGLIN+SRTI LE
Sbjct: 301 SDSTTSVAGPIPGGPTNQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINVSRTIRLE 360

Query: 361 SSAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLG 420
           SSAALVD KQRYAVNSVSFIPADTPLKLADYFNI+GVF IG IPD PS K MYLDTSVLG
Sbjct: 361 SSAALVDRKQRYAVNSVSFIPADTPLKLADYFNINGVFRIGIIPDFPSRKKMYLDTSVLG 420

Query: 421 TDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVY 480
            DYRAFIEIVFQN ENIVQSWH+DGY FWVVGMDGGVWTPASREQYNLRDAVSR TTQVY
Sbjct: 421 ADYRAFIEIVFQNHENIVQSWHLDGYNFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVY 480

Query: 481 PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRV 540
           PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQ YLRVYTPVKS RDEYNIPDNALLCG+ 
Sbjct: 481 PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQLYLRVYTPVKSLRDEYNIPDNALLCGKA 540

Query: 541 ANQSTISP 549
             +STISP
Sbjct: 541 VGRSTISP 548

BLAST of Sed0011294 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 7.4e-175
Identity = 294/538 (54.65%), Postives = 378/538 (70.26%), Query Frame = 0

Query: 14  CFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN 73
           C ++A    V  +  A+ PY    WNVTYG   PLGV QQ ILINGQFPGP+I + +N+N
Sbjct: 10  CLYLAAAATV--VVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNN 69

Query: 74  IYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYF 133
           + INV N+L EPFLL+WNGIQ RKN +QDG  GT CPI  G N+TY FQ KDQIGS+FY+
Sbjct: 70  VIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYY 129

Query: 134 PSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLP 193
           P+   H+AAGG+G +RV SR LIPVP+ +P  DYT+L+GDWYT +HT LK  LD G  + 
Sbjct: 130 PTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIG 189

Query: 194 LPNGIVINGR-----GPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGT 253
            P+GIVING+     G +A  FT++ GKTYR RI NVG++ S+NFR+Q+H+M LVE+EG+
Sbjct: 190 RPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGS 249

Query: 254 HTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVA 313
           H +QN Y SLDVHVGQ +  ++TANQ  +D+Y+  S+RF   ++T+T +L Y       +
Sbjct: 250 HVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPAS 309

Query: 314 GPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDG 373
             +P GP     WSLNQ RS R NLTAS  RPNPQGSYHYG INI+RTI L ++   VDG
Sbjct: 310 SQLPAGPVGWA-WSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 369

Query: 374 KQRYAVNSVSFIPADTPLKLADYFNI-DGVFHIGSIPDSPSG---KNMYLDTSVLGTDYR 433
           K R+A+N VS    +TPLKLA+YF I D VF   +I D P+    KN+ ++ +VL   +R
Sbjct: 370 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 429

Query: 434 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 493
            F+E+VF+N E  VQSWH+DGY+F+ V ++ G WTP  R+ YNL DAVSR+T QVYPK W
Sbjct: 430 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 489

Query: 494 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVAN 543
            AI ++ DN GMWN+RSE   R+YLGQQ Y  V +P KS RDEYN+P+ +L CG V N
Sbjct: 490 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKN 544

BLAST of Sed0011294 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-167
Identity = 284/543 (52.30%), Postives = 366/543 (67.40%), Query Frame = 0

Query: 17  VALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYI 76
           VALL  +     A+ PY +F+WNVTYG I PLGV QQGILINGQFPGP I   +N+NI +
Sbjct: 9   VALLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVV 68

Query: 77  NVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSL 136
           NV N+L EPFL +WNG+Q RKNS+QDG  GT CPI  G+NFTYRFQVKDQIGS+ YFP+ 
Sbjct: 69  NVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTT 128

Query: 137 QFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPN 196
             H+AAGG+G++ V SR LIPVPF  PA +Y + VGDWY   H TLK ILD G  +  P+
Sbjct: 129 ALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPD 188

Query: 197 GIVINGRGPNA-----TTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTM 256
           GI+ING+           FT+E GKTYR+R  N+G+  SVN R Q H M LVE+EG+HT+
Sbjct: 189 GIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTV 248

Query: 257 QNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPI 316
           QN Y SLD+HVGQ  SVL+TA+Q  +D+Y+ VS+RF  + L+S AI+ Y++     +  +
Sbjct: 249 QNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPEL 308

Query: 317 PGGP---TNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDG 376
           P  P   T  I WS+NQ RS R NLTAS  RPNPQGSYHYG INI+RTI + +S + V G
Sbjct: 309 PTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGG 368

Query: 377 KQRYAVNSVSFIPADTPLKLADYFN-IDGVFHIGSIPDSPSG--KNMYLDTSVLGTDYRA 436
           K RY +N +S    +TPLKL +YF   +  F    + D        + + T+V    YR 
Sbjct: 369 KLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRN 428

Query: 437 FIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWT 496
           F+EI+F+N E  ++++H+DGY+F+ V ++ G W+P  R+ YNL D +SR   QVYP SW 
Sbjct: 429 FVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWA 488

Query: 497 AIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQST 549
           AI ++ DN GMWN+RSE W + YLG+Q Y  V +P +S RDEYNIPDN  LCG V   S 
Sbjct: 489 AIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKGLSM 548

BLAST of Sed0011294 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.2e-151
Identity = 265/558 (47.49%), Postives = 369/558 (66.13%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVI-GIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPG 64
           MA + + L  F   L F ++  ++FA  P+  +D+ V+Y    PLGV QQ I +NGQFPG
Sbjct: 1   MAATCSLLASF--LLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPG 60

Query: 65  PDIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQV 124
           P + A TN N+ +NV N L EP LL+W GIQ R+NS+QDGV GT CPIP   NFTY+FQV
Sbjct: 61  PLLNATTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQV 120

Query: 125 KDQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLK 184
           KDQIGSFFY PSL F +A+GGFG I + +R +IP+PFP+P  +   ++GDWYT +H  L+
Sbjct: 121 KDQIGSFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALR 180

Query: 185 SILDRGHRLPLPNGIVINGRGP-----------NATTFTVEQGKTYRFRISNVGLEHSVN 244
             LD G  L +P+G++ING+GP           +  TF VE GKTYR R+ NVG+  S+N
Sbjct: 181 RALDSGKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLN 240

Query: 245 FRVQDHEMMLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQ-PSRDFYIAVSTRFASKI 304
           FR+Q+H ++LVE EG +T Q  ++  DVHVGQSYS L+T +Q  + D+YI  S RF ++ 
Sbjct: 241 FRIQNHSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNET 300

Query: 305 ----LTSTAILHYSDSTTSVAGPIPGGPTN-EIDWS-LNQARSIRTNLTASGPRPNPQGS 364
               +T  AILHYS+S   V+GP+P   T+    WS ++Q ++IR N +ASG RPNPQGS
Sbjct: 301 VWQRVTGVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGS 360

Query: 365 YHYGLINISRTITLES-SAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIP 424
           +HYG INI+ T  L S    +++G  R  +N +SF+   TP++LAD   + G + +   P
Sbjct: 361 FHYGQINITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFP 420

Query: 425 DSPSGKNMYLDTSVLGTDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASRE 484
           D P  + + LD S++   Y+ FI++VFQN +  +QS+H+DGY+F+VVGMD G+W+   + 
Sbjct: 421 DRPFNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKG 480

Query: 485 QYNLRDAVSRYTTQVYPKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSF 543
            YN  DA+SR T +VYP  WTA+ +SLDNVG+WNIR E   R YLG++ Y+R+  P +  
Sbjct: 481 SYNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDG 540

BLAST of Sed0011294 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.4e-149
Identity = 258/544 (47.43%), Postives = 361/544 (66.36%), Query Frame = 0

Query: 15  FHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNI 74
           F  AL+F   G +FA  PY  +D+ ++Y    PLGV QQ I +NG+FPGP I A TN N+
Sbjct: 12  FSFALIF---GFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNV 71

Query: 75  YINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFP 134
           ++NV N L EP LL+W G+Q R+NS+QDGV GT CPIP   NFTY FQ+KDQIGS+FY P
Sbjct: 72  HVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSP 131

Query: 135 SLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPL 194
           SL F +A+GGFG++ + +R L+P+PF EP  +   ++GDWYT NHT L+ ILD G  L +
Sbjct: 132 SLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGM 191

Query: 195 PNGIVINGRGP-----------NATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLV 254
           P+G++ING+GP              T  V+ GKTYR R+ NVG+  S+NFR+Q+H+++L+
Sbjct: 192 PDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLI 251

Query: 255 EVEGTHTMQNTYSSLDVHVGQSYSVLITANQ-PSRDFYIAVSTRFASKI----LTSTAIL 314
           E EG +T Q  ++  DVHVGQSYS L+T +Q  + D+YI  S RF ++     +T   IL
Sbjct: 252 ETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGIL 311

Query: 315 HYSDSTTSVAGPIPGGPTN-EIDWS-LNQARSIRTNLTASGPRPNPQGSYHYGLINISRT 374
           HYS+S    +GP+P   T+    WS +NQ R+I+ N +ASG RPNPQGS+HYG INI+RT
Sbjct: 312 HYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRT 371

Query: 375 ITLES-SAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNM-YL 434
             L S     ++GK R  +N +SF+   TP++LAD   + G + +   PD P  + +  L
Sbjct: 372 YILRSLPPTKINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-DFPDRPLDEKLPRL 431

Query: 435 DTSVLGTDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSR 494
            +S++   Y+ FI+++FQN +  +QS+HIDGYAF+VV MD G+W+      YN  DAV+R
Sbjct: 432 SSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVAR 491

Query: 495 YTTQVYPKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNA 539
            T +VYP +WTA+ +SLDNVG+WNIR E   R YLGQ+ Y+R+  P ++   E + P+N 
Sbjct: 492 STVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENV 551

BLAST of Sed0011294 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 266/549 (48.45%), Postives = 354/549 (64.48%), Query Frame = 0

Query: 15  FHVALL--FGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTND 74
           F + LL  F  I   FA  PY F+++ V+Y    PLGV QQ I ING+FPGP I   TN+
Sbjct: 4   FKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNE 63

Query: 75  NIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFY 134
           N+ +NV N L E  LL WNGIQQR+ S+QDGV GT CPIP   N+TY FQVKDQIGSFFY
Sbjct: 64  NLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFY 123

Query: 135 FPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRL 194
           FPSL F +A+GGFGS  V  R +IPVPF  P  D T+ +GDWY  NHT L+  LD G  L
Sbjct: 124 FPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDL 183

Query: 195 PLPNGIVINGRGP------------NATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEM 254
            +P+G++ING+GP            +  T TV  GKTYR R+SNVG+  S+NFR+Q H +
Sbjct: 184 GMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNL 243

Query: 255 MLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQ-PSRDFYIAVSTRFASKIL----TST 314
           +L E EG++T+Q  Y+SLD+HVGQSYS L+T +Q  S D+YI  S R  ++ +    T  
Sbjct: 244 VLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGV 303

Query: 315 AILHYSDSTTSVAGPIPGGPTNEID--WSLNQARSIRTNLTASGPRPNPQGSYHYGLINI 374
            IL Y++S     G +P GP +E D  +S+NQARSIR N++ASG RPNPQGS+ YG IN+
Sbjct: 304 GILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINV 363

Query: 375 SRTITLESSAAL-VDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNM 434
           +    L +   + + GK+R  +N +SF    TP++LAD   +  V+ +   P  P     
Sbjct: 364 TDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGPA 423

Query: 435 YLDTSVLGTDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAV 494
            + TS++   YR F+E+V QN +  +QS+H+ GYAF+VVGMD G WT  SR  YN  D +
Sbjct: 424 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 483

Query: 495 SRYTTQVYPKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPD 542
           +R T QVYP +W+AI +SLDN G WN+R+E     YLGQ+ Y+RV  P ++ + E+  PD
Sbjct: 484 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 543

BLAST of Sed0011294 vs. ExPASy TrEMBL
Match: A0A6J1I1G1 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111468166 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 7.7e-300
Identity = 492/544 (90.44%), Postives = 515/544 (94.67%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 64
           MA   A+L CF+VAL  G +GIA ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP
Sbjct: 1   MAPKPAELWCFYVALALGFMGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 60

Query: 65  DIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVK 124
           +IYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP GKNFTYR QVK
Sbjct: 61  EIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVK 120

Query: 125 DQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKS 184
           DQIG++FYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANH+ LK+
Sbjct: 121 DQIGTYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKT 180

Query: 185 ILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVE 244
           ILD GHRLP PNGIVINGRGP ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM LVE
Sbjct: 181 ILDGGHRLPNPNGIVINGRGPQATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVE 240

Query: 245 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDST 304
           VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKIL STAILHYSDST
Sbjct: 241 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILNSTAILHYSDST 300

Query: 305 TSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAA 364
           TSV+GPIPGGPTNEIDWSLNQARS+RTNLTASGPRPNPQGSYHYGLINISRTI LESSAA
Sbjct: 301 TSVSGPIPGGPTNEIDWSLNQARSVRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAA 360

Query: 365 LVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYR 424
           LVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IGSIPD PSGK MYLDTSV+G DYR
Sbjct: 361 LVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGSIPDFPSGKKMYLDTSVMGADYR 420

Query: 425 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 484
           AFIEIVFQN ENIVQSWH+DGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYPKSW
Sbjct: 421 AFIEIVFQNHENIVQSWHLDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPKSW 480

Query: 485 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQS 544
           TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKS RDEYN+PDNALLCG+ A +S
Sbjct: 481 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSLRDEYNVPDNALLCGKAAGRS 540

Query: 545 TISP 549
           T SP
Sbjct: 541 TASP 544

BLAST of Sed0011294 vs. ExPASy TrEMBL
Match: A0A6J1GK88 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455076 PE=3 SV=1)

HSP 1 Score: 1033.5 bits (2671), Expect = 3.2e-298
Identity = 489/544 (89.89%), Postives = 514/544 (94.49%), Query Frame = 0

Query: 5   MAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 64
           MA   A+L CF+VA+  G IGIA ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP
Sbjct: 1   MAPKPAQLWCFYVAMALGFIGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGP 60

Query: 65  DIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVK 124
           +IYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP GKNFTYR QVK
Sbjct: 61  EIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVK 120

Query: 125 DQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKS 184
           DQIG++FYFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANH+ LK 
Sbjct: 121 DQIGTYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKI 180

Query: 185 ILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVE 244
           ILD GHRLP PNGIVINGRGP ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHEM LVE
Sbjct: 181 ILDGGHRLPNPNGIVINGRGPLATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVE 240

Query: 245 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDST 304
           VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKIL STAILHYSDST
Sbjct: 241 VEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILNSTAILHYSDST 300

Query: 305 TSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAA 364
           TSV+GPIPGGPTNEIDWSLNQARS+RTNLTASGPRPNPQGSYHYGLINISRTI LESSAA
Sbjct: 301 TSVSGPIPGGPTNEIDWSLNQARSVRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAA 360

Query: 365 LVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYR 424
           LVD KQRYAVNSVSFIPADTPLKLADYFNIDGVF IG+IPD PSGK MYLDTSV+G DYR
Sbjct: 361 LVDRKQRYAVNSVSFIPADTPLKLADYFNIDGVFRIGTIPDFPSGKKMYLDTSVMGADYR 420

Query: 425 AFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSW 484
           AFIEIVFQN ENIVQSWH+DGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYP+SW
Sbjct: 421 AFIEIVFQNHENIVQSWHLDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPRSW 480

Query: 485 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQS 544
           TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKS RDEYN+PDNALLCG+ A +S
Sbjct: 481 TAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSLRDEYNVPDNALLCGKAAGRS 540

Query: 545 TISP 549
           T +P
Sbjct: 541 TATP 544

BLAST of Sed0011294 vs. ExPASy TrEMBL
Match: A0A6J1C5Y9 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111007682 PE=3 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 4.4e-295
Identity = 489/548 (89.23%), Postives = 512/548 (93.43%), Query Frame = 0

Query: 1   MVVKMAKSSAKLCCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQ 60
           MV  +A+ + KL CF V  +  +I IAF+DSPYRFFDWNVTYGDIYPLGVRQQGILINGQ
Sbjct: 1   MVEIVAQRTPKLWCFIVFWMSVMINIAFSDSPYRFFDWNVTYGDIYPLGVRQQGILINGQ 60

Query: 61  FPGPDIYAVTNDNIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYR 120
           FPGPDIYAVTNDN+YINVHNSLPEPFLLSW+G+QQRKNSYQDGVYGTTCPIP GKNFTYR
Sbjct: 61  FPGPDIYAVTNDNLYINVHNSLPEPFLLSWSGVQQRKNSYQDGVYGTTCPIPPGKNFTYR 120

Query: 121 FQVKDQIGSFFYFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHT 180
            QVKDQIGSFFYFPSL  HKAAGGFGSIRVLSRPLIPVPFP+PA D+TLL+GDWYT NH 
Sbjct: 121 IQVKDQIGSFFYFPSLALHKAAGGFGSIRVLSRPLIPVPFPDPAGDFTLLIGDWYTTNHK 180

Query: 181 TLKSILDRGHRLPLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEM 240
           TL++ILDRGHRLP PNGIVINGRGPNATTFTVE+GKTYRFRISNVGLEHS+NFRVQDHEM
Sbjct: 181 TLQTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLEHSLNFRVQDHEM 240

Query: 241 MLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHY 300
            LVEVEGTHTMQNTYSSLDVHVGQSYSVLITA+QPSRDFYIAVSTRFASKILTSTAILHY
Sbjct: 241 KLVEVEGTHTMQNTYSSLDVHVGQSYSVLITADQPSRDFYIAVSTRFASKILTSTAILHY 300

Query: 301 SDSTTSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLE 360
           SDSTTSVAGPIPGGPTN+IDWSLNQARSIRTNLTASGPRPNPQGSYHYGLIN+SRTI LE
Sbjct: 301 SDSTTSVAGPIPGGPTNQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINVSRTIRLE 360

Query: 361 SSAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLG 420
           SSAALVD KQRYAVNSVSFIPADTPLKLADYFNI+GVF IG IPD PS K MYLDTSVLG
Sbjct: 361 SSAALVDRKQRYAVNSVSFIPADTPLKLADYFNINGVFRIGIIPDFPSRKKMYLDTSVLG 420

Query: 421 TDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVY 480
            DYRAFIEIVFQN ENIVQSWH+DGY FWVVGMDGGVWTPASREQYNLRDAVSR TTQVY
Sbjct: 421 ADYRAFIEIVFQNHENIVQSWHLDGYNFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVY 480

Query: 481 PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRV 540
           PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQ YLRVYTPVKS RDEYNIPDNALLCG+ 
Sbjct: 481 PKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQLYLRVYTPVKSLRDEYNIPDNALLCGKA 540

Query: 541 ANQSTISP 549
             +STISP
Sbjct: 541 VGRSTISP 548

BLAST of Sed0011294 vs. ExPASy TrEMBL
Match: A0A1S3BIH0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103490248 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.3e-294
Identity = 484/535 (90.47%), Postives = 512/535 (95.70%), Query Frame = 0

Query: 15  FHVALLFGV-IGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN 74
           F+VAL+ G+ I IAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN
Sbjct: 13  FNVALVLGLFINIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN 72

Query: 75  IYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYF 134
           IYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIP G+NFTYR QVKDQIGS+FYF
Sbjct: 73  IYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRIQVKDQIGSYFYF 132

Query: 135 PSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLP 194
           PSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANHTTLK+ILDRGHRLP
Sbjct: 133 PSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTTLKTILDRGHRLP 192

Query: 195 LPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQN 254
            PNGIVINGRGP+ATTFTVE+GKTYRFRI+NVGL+HS+NFRVQDHE+ LVEVEGTHTMQN
Sbjct: 193 FPNGIVINGRGPSATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHELKLVEVEGTHTMQN 252

Query: 255 TYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIPG 314
           TYSSLDVHVGQSYSVLITA+QPSRDFYIAVSTRFA K+LTSTAILHYSDSTTSV GPIPG
Sbjct: 253 TYSSLDVHVGQSYSVLITADQPSRDFYIAVSTRFAPKVLTSTAILHYSDSTTSVGGPIPG 312

Query: 315 GPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYA 374
           GPT E++WS+NQARSIRTNLTASGPRPNPQGSYHYGLINISRTI LESSAALV+ KQRYA
Sbjct: 313 GPT-EVEWSINQARSIRTNLTASGPRPNPQGSYHYGLINISRTIKLESSAALVNRKQRYA 372

Query: 375 VNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYRAFIEIVFQN 434
           VNSVSFIPADTPLKLADYF+IDGVF IGSIPD PSGK M+LDTSV+G DYRAFIEIVFQN
Sbjct: 373 VNSVSFIPADTPLKLADYFSIDGVFRIGSIPDFPSGKTMFLDTSVMGADYRAFIEIVFQN 432

Query: 435 RENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLDN 494
            ENIVQSWHIDGY+FWVVGMDGGVWTPASREQYNLRDAVSR TTQVYPKSWTAIY+SLDN
Sbjct: 433 HENIVQSWHIDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTTQVYPKSWTAIYMSLDN 492

Query: 495 VGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQSTISP 549
           VGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYN+PDNALLCG+   +ST SP
Sbjct: 493 VGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNVPDNALLCGKADGKSTASP 546

BLAST of Sed0011294 vs. ExPASy TrEMBL
Match: A0A5A7TA42 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold460G00620 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.3e-278
Identity = 474/551 (86.03%), Postives = 501/551 (90.93%), Query Frame = 0

Query: 15  FHVALLFGV-IGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN 74
           F+VAL+ G+ I IAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN
Sbjct: 13  FNVALVLGLFINIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDN 72

Query: 75  IYINVHNSLPEPFLLS--WNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFF 134
           IYINVHN+L   F  S   NG+QQRKNSYQDGVYGTTCPIP G+NFTYR QVKDQIGS+F
Sbjct: 73  IYINVHNTL---FCESKRRNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRIQVKDQIGSYF 132

Query: 135 YFPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHR 194
           YFPSL FHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLL+GDWYTANHTTLK+ILDRGHR
Sbjct: 133 YFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTTLKTILDRGHR 192

Query: 195 LPLPNGIVINGRGP------NATT--------FTVEQGKTYRFRISNVGLEHSVNFRVQD 254
           LP PNGIVINGRGP      N T         F V  GKTYRFRI+NVGL+HS+NFRVQD
Sbjct: 193 LPFPNGIVINGRGPRLIFDWNGTIRSDFFGFFFVVVVGKTYRFRITNVGLQHSLNFRVQD 252

Query: 255 HEMMLVEVEGTHTMQNTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAI 314
           HE+ LVEVEGTHTMQNTYSSLDVHVGQSYSVLITA+QPSRDFYIAVSTRFA K+LTSTAI
Sbjct: 253 HELKLVEVEGTHTMQNTYSSLDVHVGQSYSVLITADQPSRDFYIAVSTRFAPKVLTSTAI 312

Query: 315 LHYSDSTTSVAGPIPGGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTI 374
           LHYSDSTTSV GPIPGGPT E++WS+NQARSIRTNLTASGPRPNPQGSYHYGLINISRTI
Sbjct: 313 LHYSDSTTSVGGPIPGGPT-EVEWSINQARSIRTNLTASGPRPNPQGSYHYGLINISRTI 372

Query: 375 TLESSAALVDGKQRYAVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTS 434
            LESSAALV+ KQRYAVNSVSFIPADTPLKLADYF+IDGVF IGSIPD PSGK M+LDTS
Sbjct: 373 KLESSAALVNRKQRYAVNSVSFIPADTPLKLADYFSIDGVFRIGSIPDFPSGKTMFLDTS 432

Query: 435 VLGTDYRAFIEIVFQNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTT 494
           V+G DYRAFIEIVFQN ENIVQSWHIDGY+FWVVGMDGGVWTPASREQYNLRDAVSR TT
Sbjct: 433 VMGADYRAFIEIVFQNHENIVQSWHIDGYSFWVVGMDGGVWTPASREQYNLRDAVSRCTT 492

Query: 495 QVYPKSWTAIYVSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLC 549
           QVYPKSWTAIY+SLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYN+PDNALLC
Sbjct: 493 QVYPKSWTAIYMSLDNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNVPDNALLC 552

BLAST of Sed0011294 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 836.3 bits (2159), Expect = 1.5e-242
Identity = 388/529 (73.35%), Postives = 451/529 (85.26%), Query Frame = 0

Query: 17  VALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYI 76
           ++LLF V     A+ PYRFF+WN+TYGDIYPLGVRQQGILING FPGPDI++VTNDN+ I
Sbjct: 15  LSLLFAVT----AEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLII 74

Query: 77  NVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSL 136
           NV+NSL EPFLLSWNGIQQR+NS+ DGVYGTTCPIP GKN+TY  Q+KDQIGSF+YFPSL
Sbjct: 75  NVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSL 134

Query: 137 QFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPN 196
            FHKAAGGFG IR+LSRP IPVPFP+PA D T+L+GDWY ANHT L++ LD G +LPLP+
Sbjct: 135 GFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPD 194

Query: 197 GIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQNTYS 256
           GI+INGR   A T  VEQGKTYRFRISNVGL+ S+NFR+QDH+M +VEVEGTHT+Q T+S
Sbjct: 195 GILINGRSSGA-TLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFS 254

Query: 257 SLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIPGGPT 316
           SLDVHVGQSYSVL+TA+Q  RD+Y+ VS+RF S +LT+T I  YS+S   V+GPIPGGPT
Sbjct: 255 SLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPT 314

Query: 317 NEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYAVNS 376
            +IDWSLNQAR+IRTNL+ASGPRPNPQGSYHYG+IN +RTI L SSA  VDGKQRYAVNS
Sbjct: 315 IQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNS 374

Query: 377 VSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYRAFIEIVFQNREN 436
           VSF PADTPLK+ADYF IDGV+  GSI   P+G  +YLDTSV+  DYR F+EI+F+N E+
Sbjct: 375 VSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSED 434

Query: 437 IVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLDNVGM 496
           IVQSWH+DGY+FWVVGMDGG W+P SR +YNLRDAV+R T QVYP SWTAI ++LDNVGM
Sbjct: 435 IVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGM 494

Query: 497 WNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQST 546
           WN+RSEFWARQYLGQQ YLRVYTP  S RDEY IP NALLCGR + +ST
Sbjct: 495 WNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRST 538

BLAST of Sed0011294 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 814.7 bits (2103), Expect = 4.5e-236
Identity = 378/517 (73.11%), Postives = 434/517 (83.95%), Query Frame = 0

Query: 29  ADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYINVHNSLPEPFLL 88
           A+SPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDI++VTNDN+ INVHNSL EPFL+
Sbjct: 24  AESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVHNSLDEPFLI 83

Query: 89  SWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSLQFHKAAGGFGSI 148
           SWNG+Q R+NSY DG+YGTTCPIP   N+TY  QVKDQIGSF+YFPSL FHKAAGGFG I
Sbjct: 84  SWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPSLAFHKAAGGFGGI 143

Query: 149 RVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPNGIVINGRGPNAT 208
           R+LSRP IPVPF +PA DYT+L+GDWY  NHT LKS LDRG +LP P+GI+INGR  N  
Sbjct: 144 RILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSPDGILINGRS-NGA 203

Query: 209 TFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQNTYSSLDVHVGQSYSV 268
           T  VEQGKTYR RISNVGL+ S+NFR+Q+H M LVEVEGTHT+Q  +SSLDVHVGQSYSV
Sbjct: 204 TLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTMFSSLDVHVGQSYSV 263

Query: 269 LITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIPGGPTNEIDWSLNQARS 328
           LITA+Q  RD+Y+ VS+RF  KI+T+T +L YS S+T  +GPIPGGPT ++DWSLNQAR+
Sbjct: 264 LITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGGPTIQVDWSLNQARA 323

Query: 329 IRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYAVNSVSFIPADTPLKL 388
           IRTNLTASGPRPNPQGSYHYGLI + RTI   SSA  ++GKQRY VNSVSF+PADTPLKL
Sbjct: 324 IRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGVNSVSFVPADTPLKL 383

Query: 389 ADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYRAFIEIVFQNRENIVQSWHIDGYAF 448
           AD+F I GV+ I SI D P+   +YLDTSVL  DYR FIEIVF+N+E+IVQS+H++GY+F
Sbjct: 384 ADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQEDIVQSYHLNGYSF 443

Query: 449 WVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLDNVGMWNIRSEFWARQY 508
           WVVGMDGG W   SR  YNLRDAVSR T QVYPKSWTAIY++LDNVGMWN+RSEFWARQY
Sbjct: 444 WVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNVGMWNLRSEFWARQY 503

Query: 509 LGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQST 546
           LGQQ YLRV+T   S RDEY IP N+ LCGR   + T
Sbjct: 504 LGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHT 539

BLAST of Sed0011294 vs. TAIR 10
Match: AT4G38420.1 (SKU5 similar 9 )

HSP 1 Score: 793.9 bits (2049), Expect = 8.3e-230
Identity = 366/533 (68.67%), Postives = 440/533 (82.55%), Query Frame = 0

Query: 19  LLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYINV 78
           +   +I    AD PYRFFDW VTYG+I PLG+ Q+GILINGQ+PGPDIY+VTNDN+ INV
Sbjct: 15  MTISIISFVQADDPYRFFDWRVTYGNISPLGIPQRGILINGQYPGPDIYSVTNDNLIINV 74

Query: 79  HNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSLQF 138
           HN L EPFLLSWNG+Q RKNSYQDGVYGTTCPIP GKN+TY  QVKDQIGSFFYFPSL  
Sbjct: 75  HNDLDEPFLLSWNGVQLRKNSYQDGVYGTTCPIPPGKNYTYAIQVKDQIGSFFYFPSLAV 134

Query: 139 HKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPNGI 198
           HKAAGGFG  R+LSRP IPVPFPEPA D+T L+GDW+  +H  LK+ILDRGH+LPLP G+
Sbjct: 135 HKAAGGFGGFRILSRPRIPVPFPEPAGDFTFLIGDWFKHDHKVLKAILDRGHKLPLPQGV 194

Query: 199 VINGRGPN-ATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQNTYSS 258
           +ING+G +  ++ TV +GKTYRFRISNVGL+H++NFR+Q H+M LVEVEGTHT+Q+ Y+S
Sbjct: 195 LINGQGVSYMSSITVHKGKTYRFRISNVGLQHTLNFRIQGHQMKLVEVEGTHTVQSMYTS 254

Query: 259 LDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDS----TTSVAGPIPG 318
           LD+HVGQSYSVL+T +QP +D+ I VST+F +K L  ++ +HYS+S    ++S       
Sbjct: 255 LDIHVGQSYSVLVTMDQPDQDYDIVVSTKFVAKKLLVSSTIHYSNSRHSHSSSANSVHVQ 314

Query: 319 GPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYA 378
            P +E+DWS+ QARSIRTNLTASGPRPNPQGSYHYG I ISRT+ LESSAALV  KQRYA
Sbjct: 315 QPADELDWSIKQARSIRTNLTASGPRPNPQGSYHYGRIKISRTLILESSAALVKRKQRYA 374

Query: 379 VNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSP-SGKNMYLDTSVLGTDYRAFIEIVFQ 438
           +N VSF+P DTPLKLADYF I GVF +GSIPD P  G+ M ++TSV+G  +R F+EI+FQ
Sbjct: 375 INGVSFVPGDTPLKLADYFKIKGVFKMGSIPDKPRRGRGMRMETSVMGAHHRDFLEIIFQ 434

Query: 439 NRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLD 498
           NRE IVQS+H+DGY+FWVVG D G W+ ASR +YNLRDA+SR TTQVYP+SWTA+YV+LD
Sbjct: 435 NREKIVQSYHLDGYSFWVVGTDRGTWSKASRREYNLRDAISRSTTQVYPESWTAVYVALD 494

Query: 499 NVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQST 546
           NVGMWN+RSE+WARQYLGQQFYLRVY+P  S RDEY +P NALLCGR +N+ T
Sbjct: 495 NVGMWNLRSEYWARQYLGQQFYLRVYSPTHSLRDEYLLPKNALLCGRASNKHT 547

BLAST of Sed0011294 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 775.0 bits (2000), Expect = 4.0e-224
Identity = 360/526 (68.44%), Postives = 433/526 (82.32%), Query Frame = 0

Query: 17  VALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTNDNIYI 76
           +  L  +I +AFA+ PYRFF+W+VTYG+I PLGV QQGILING+FPGPDI ++TNDN+ I
Sbjct: 12  ITTLLFLISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNLII 71

Query: 77  NVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFYFPSL 136
           NV N L EPFLLSWNGI+  KNS+QDGVYGT CPIP GKN+TY  QVKDQIGSF+YFPSL
Sbjct: 72  NVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFPSL 131

Query: 137 QFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRLPLPN 196
            FHKAAGGFG IR+ SR LIPVPFP PA DYTLLVGDWY  NH  LK+ LD G +LPLP+
Sbjct: 132 GFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLPD 191

Query: 197 GIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQNTYS 256
           GI+INGR   A T  +E GKTYR RISNVGL++S+NFR+Q+H M LVEVEG +T+QN +S
Sbjct: 192 GILINGRSSGA-TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLFS 251

Query: 257 SLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIPGGPT 316
           SLDVHVGQSYSVLITA+QP++D+Y+ VS+RF SKILT+T +LHYS+S   V+GPIP GP 
Sbjct: 252 SLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGPI 311

Query: 317 NEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRYAVNS 376
            ++ WS NQAR+IRTNLTASGPRPNPQGSY YG+INI+RTI L ++   ++GKQRYAVNS
Sbjct: 312 -KLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVNS 371

Query: 377 VSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKNMYLDTSVLGTDYRAFIEIVFQNREN 436
            SF PADTPLKL DYF IDGV+  GSI D P+   ++  TSV+  D+RAF+E++F+N E+
Sbjct: 372 ASFYPADTPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFRAFVEVIFENSED 431

Query: 437 IVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSLDNVGM 496
           IVQSWH+DGY+F+VVGM+ G W+PASR+ YNL DA+ R T QVYP+SWTAIY++LDNVGM
Sbjct: 432 IVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVGM 491

Query: 497 WNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVAN 543
           WN+RSE W RQYLGQQFY+RVYT   S RDEY IP NALLCGR ++
Sbjct: 492 WNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRASS 535

BLAST of Sed0011294 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 774.6 bits (1999), Expect = 5.2e-224
Identity = 364/534 (68.16%), Postives = 427/534 (79.96%), Query Frame = 0

Query: 13  CCFHVALLFGVIGIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYAVTND 72
           C   + LL  +I     D+PYRFF W +TYGDIYPLGV+QQGILINGQFPGP I A+TND
Sbjct: 5   CKVSIVLLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITND 64

Query: 73  NIYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPAGKNFTYRFQVKDQIGSFFY 132
           NI I+V N L EPFL+SWNG+QQRKNS+QDGV GTTCPIP GKNFTY  QVKDQIGSF+Y
Sbjct: 65  NIIISVFNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYY 124

Query: 133 FPSLQFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLVGDWYTANHTTLKSILDRGHRL 192
           FPSL FHKAAG FG+IRV SRP IPVPF  P  D+ LL GDWY  NH  L+ +L+ G  L
Sbjct: 125 FPSLAFHKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNL 184

Query: 193 PLPNGIVINGRGPNATTFTVEQGKTYRFRISNVGLEHSVNFRVQDHEMMLVEVEGTHTMQ 252
           P P+G++INGRG    TFTV+ GKTYRFRISNVG+  S+NFR+Q H M LVEVEG+HT+Q
Sbjct: 185 PNPDGVLINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQ 244

Query: 253 NTYSSLDVHVGQSYSVLITANQPSRDFYIAVSTRFASKILTSTAILHYSDSTTSVAGPIP 312
           N Y+SLD+H+GQSYSVL+TANQ  +D+YI +S+RF  K+LT+T+ILHYS+S   V+GP+P
Sbjct: 245 NIYTSLDIHLGQSYSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVP 304

Query: 313 GGPTNEIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINISRTITLESSAALVDGKQRY 372
            GPT +I  SL QAR+IR NLTASGPRPNPQGSYHYGLI   RTI L +SA  ++GKQRY
Sbjct: 305 NGPTLDIASSLYQARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRY 364

Query: 373 AVNSVSFIPADTPLKLADYFNIDGVFHIGSIPDSPSGKN-MYLDTSVLGTDYRAFIEIVF 432
           AVN  SF+  DTPLKLADYF I GVF++GSIP SPSG N  YL +SV+  ++R FIE+VF
Sbjct: 365 AVNGASFVAPDTPLKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVF 424

Query: 433 QNRENIVQSWHIDGYAFWVVGMDGGVWTPASREQYNLRDAVSRYTTQVYPKSWTAIYVSL 492
           QN EN VQSWH+ GY+F+VVGMDGG WTP SR +YNLRDAVSR T QVYP++WTAIY++L
Sbjct: 425 QNWENSVQSWHVSGYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIAL 484

Query: 493 DNVGMWNIRSEFWARQYLGQQFYLRVYTPVKSFRDEYNIPDNALLCGRVANQST 546
           DNVGMWNIRSE WARQYLGQQFYLRVYT   S+RDEY  P NAL+CGR   + T
Sbjct: 485 DNVGMWNIRSENWARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRHT 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969054.11.6e-29990.44L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_022952372.16.7e-29889.89L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6572144.1 hypothetical prot... [more]
XP_023554680.12.1e-29689.71L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
XP_038888634.13.7e-29689.56L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_022135823.19.0e-29589.23L-ascorbate oxidase homolog [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q006247.4e-17554.65L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291621.5e-16752.30L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q8VXX51.2e-15147.49Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN61.4e-14947.43Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q9SU402.0e-14848.45Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1I1G17.7e-30090.44L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111468166 PE=3 SV=... [more]
A0A6J1GK883.2e-29889.89L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455076 PE=3 S... [more]
A0A6J1C5Y94.4e-29589.23L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111007682 PE=3 ... [more]
A0A1S3BIH06.3e-29490.47L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103490248 PE=3 SV=1[more]
A0A5A7TA421.3e-27886.03L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT1G76160.11.5e-24273.35SKU5 similar 5 [more]
AT1G41830.14.5e-23673.11SKU5-similar 6 [more]
AT4G38420.18.3e-23068.67SKU5 similar 9 [more]
AT1G21860.14.0e-22468.44SKU5 similar 7 [more]
AT4G22010.15.2e-22468.16SKU5 similar 4 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 32..154
e-value: 3.3E-32
score: 113.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 349..546
e-value: 2.1E-34
score: 121.0
IPR008972CupredoxinGENE3D2.60.40.420coord: 164..339
e-value: 1.2E-36
score: 128.7
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 350..539
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 160..340
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 28..180
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 166..302
e-value: 4.7E-39
score: 134.1
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 384..520
e-value: 1.6E-26
score: 92.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 40..153
e-value: 4.3E-38
score: 130.0
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 20..541
NoneNo IPR availablePANTHERPTHR11709:SF267MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN-RELATEDcoord: 20..541
IPR034275Ascorbate oxidase homologue, third cupredoxin domainCDDcd13894CuRO_3_AAO_like_1coord: 381..502
e-value: 6.92082E-69
score: 215.757
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 35..150
e-value: 5.16344E-73
score: 226.52

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0011294.1Sed0011294.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity