Sed0011127 (gene) Chayote v1

Overview
NameSed0011127
Typegene
OrganismSechium edule (Chayote v1)
DescriptionEF-hand calcium-binding domain-containing protein 4A
LocationLG12: 5535555 .. 5536330 (-)
RNA-Seq ExpressionSed0011127
SyntenySed0011127
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAGATTCAACTTTCCACAAAAAAAAGTGTTAAAAAACAGTAAAAAATAGTAGTTTCAGCTCATTTATATAGTTCAACCCCCAAGGGAGACTGATGGTTTTATGTGACAAAAACAGAACCCACATTTCAAATGCCGGGGTTCGGTGTTTTCCCTCTCGGTAGCTGTTTCGATGGCTGCCGAGACCACGCTCAGGGGTCTGGATTCGGCACGCGAATATGGAACTCGAGCGACAGGCCGGTCGAGCTGCAGATAAGGGTGGGATCAATATTGAAAAAAGTGCATAGTTTGAAGCCAGGATGCTCAAAGAGGTTGAAATGTAAGAGCATTTACAAAGCATACAAGCCAGGAAGGAGTATGAATAGCTTGCTTTATTACTATGATGAAGCTTGCCAACCTTATGTTTGGATACATGAAAGTGGAGGTGATTCCATGAGAATGGTGAAGCAACAGTTCATCAGTCTTGAAGATTTAAGGGAGTTTTCTGAGATCAGAATCTTTAGAGATCATCAGCGCGGTTGCGTGTCGGTTCGGAAGAAACCGAGGCCTGATTTTTGCTGATTCTTAAATACTAAACCATGTTGCGTGTCTAAACTTTTGTAGCTTTTATATGAATGTTGTTCTTGTTTTTTTTGAGTCTGTAATGCTGTTGCCAAACTGTCTTTCCCCTGTTTCCTTGTTTTGGTTTGGAATTGAAGAAAATCAGATGGCATTATTGGTGGTGTGCTTTTAAACACCCAGAATTGTTTGTTTCTTTGATTTTGATTTCTAGTCGAT

mRNA sequence

ATCAGATTCAACTTTCCACAAAAAAAAGTGTTAAAAAACAGTAAAAAATAGTAGTTTCAGCTCATTTATATAGTTCAACCCCCAAGGGAGACTGATGGTTTTATGTGACAAAAACAGAACCCACATTTCAAATGCCGGGGTTCGGTGTTTTCCCTCTCGGTAGCTGTTTCGATGGCTGCCGAGACCACGCTCAGGGGTCTGGATTCGGCACGCGAATATGGAACTCGAGCGACAGGCCGGTCGAGCTGCAGATAAGGGTGGGATCAATATTGAAAAAAGTGCATAGTTTGAAGCCAGGATGCTCAAAGAGGTTGAAATGTAAGAGCATTTACAAAGCATACAAGCCAGGAAGGAGTATGAATAGCTTGCTTTATTACTATGATGAAGCTTGCCAACCTTATGTTTGGATACATGAAAGTGGAGGTGATTCCATGAGAATGGTGAAGCAACAGTTCATCAGTCTTGAAGATTTAAGGGAGTTTTCTGAGATCAGAATCTTTAGAGATCATCAGCGCGGTTGCGTGTCGGTTCGGAAGAAACCGAGGCCTGATTTTTGCTGATTCTTAAATACTAAACCATGTTGCGTGTCTAAACTTTTGTAGCTTTTATATGAATGTTGTTCTTGTTTTTTTTGAGTCTGTAATGCTGTTGCCAAACTGTCTTTCCCCTGTTTCCTTGTTTTGGTTTGGAATTGAAGAAAATCAGATGGCATTATTGGTGGTGTGCTTTTAAACACCCAGAATTGTTTGTTTCTTTGATTTTGATTTCTAGTCGAT

Coding sequence (CDS)

ATGCCGGGGTTCGGTGTTTTCCCTCTCGGTAGCTGTTTCGATGGCTGCCGAGACCACGCTCAGGGGTCTGGATTCGGCACGCGAATATGGAACTCGAGCGACAGGCCGGTCGAGCTGCAGATAAGGGTGGGATCAATATTGAAAAAAGTGCATAGTTTGAAGCCAGGATGCTCAAAGAGGTTGAAATGTAAGAGCATTTACAAAGCATACAAGCCAGGAAGGAGTATGAATAGCTTGCTTTATTACTATGATGAAGCTTGCCAACCTTATGTTTGGATACATGAAAGTGGAGGTGATTCCATGAGAATGGTGAAGCAACAGTTCATCAGTCTTGAAGATTTAAGGGAGTTTTCTGAGATCAGAATCTTTAGAGATCATCAGCGCGGTTGCGTGTCGGTTCGGAAGAAACCGAGGCCTGATTTTTGCTGA

Protein sequence

MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKRLKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEIRIFRDHQRGCVSVRKKPRPDFC
Homology
[Close]
BLAST of Sed0011127 vs. NCBI nr
Match: XP_008438723.1 (PREDICTED: uncharacterized protein LOC103483746 [Cucumis melo] >KAA0049398.1 uncharacterized protein E6C27_scaffold171G006430 [Cucumis melo var. makuwa] >TYK17161.1 uncharacterized protein E5676_scaffold434G00050 [Cucumis melo var. makuwa])

HSP 1 Score: 294.7 bits (753), Expect = 4.4e-76
Identity = 134/142 (94.37%), Postives = 138/142 (97.18%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTR+WN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRVWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ++SLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYVSLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGC+SVRKKPRPDFC
Sbjct: 121 RIFRDHQRGCISVRKKPRPDFC 142

[Close]
BLAST of Sed0011127 vs. NCBI nr
Match: XP_011650993.1 (uncharacterized protein LOC105434839 [Cucumis sativus] >KGN57027.1 hypothetical protein Csa_009946 [Cucumis sativus])

HSP 1 Score: 292.0 bits (746), Expect = 2.8e-75
Identity = 134/142 (94.37%), Postives = 137/142 (96.48%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ++SLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYVSLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGC+SVRKK RPDFC
Sbjct: 121 RIFRDHQRGCISVRKKQRPDFC 142

[Close]
BLAST of Sed0011127 vs. NCBI nr
Match: XP_038875149.1 (uncharacterized protein LOC120067683 [Benincasa hispida])

HSP 1 Score: 291.6 bits (745), Expect = 3.7e-75
Identity = 134/142 (94.37%), Postives = 137/142 (96.48%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRPVELQIRVGSILK+VH+LKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPVELQIRVGSILKRVHNLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ+ISLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYISLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGCVSV KKPRPDFC
Sbjct: 121 RIFRDHQRGCVSVHKKPRPDFC 142

[Close]
BLAST of Sed0011127 vs. NCBI nr
Match: XP_022936946.1 (uncharacterized protein LOC111443378 [Cucurbita moschata] >KAG6597467.1 hypothetical protein SDJN03_10647, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028926.1 hypothetical protein SDJN02_10109, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 290.4 bits (742), Expect = 8.3e-75
Identity = 136/142 (95.77%), Postives = 136/142 (95.77%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGCVSVRKKPR  FC
Sbjct: 121 RIFRDHQRGCVSVRKKPRLGFC 142

[Close]
BLAST of Sed0011127 vs. NCBI nr
Match: XP_023522910.1 (uncharacterized protein LOC111786960 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.0 bits (741), Expect = 1.1e-74
Identity = 135/142 (95.07%), Postives = 136/142 (95.77%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRP+ELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPIELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGCVSVRKKPR  FC
Sbjct: 121 RIFRDHQRGCVSVRKKPRLGFC 142

[Close]
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match: A0A5A7U2C9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00050 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.1e-76
Identity = 134/142 (94.37%), Postives = 138/142 (97.18%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTR+WN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRVWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ++SLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYVSLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGC+SVRKKPRPDFC
Sbjct: 121 RIFRDHQRGCISVRKKPRPDFC 142

[Close]
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match: A0A1S3AWR3 (uncharacterized protein LOC103483746 OS=Cucumis melo OX=3656 GN=LOC103483746 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.1e-76
Identity = 134/142 (94.37%), Postives = 138/142 (97.18%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTR+WN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRVWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ++SLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYVSLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGC+SVRKKPRPDFC
Sbjct: 121 RIFRDHQRGCISVRKKPRPDFC 142

[Close]
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match: A0A0A0L8V0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150060 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.4e-75
Identity = 134/142 (94.37%), Postives = 137/142 (96.48%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQ++SLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQYVSLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGC+SVRKK RPDFC
Sbjct: 121 RIFRDHQRGCISVRKKQRPDFC 142

[Close]
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match: A0A6J1F8W2 (uncharacterized protein LOC111443378 OS=Cucurbita moschata OX=3662 GN=LOC111443378 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.0e-75
Identity = 136/142 (95.77%), Postives = 136/142 (95.77%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDHAQ SGFGTRIWN SDRPVELQIRVGSILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHAQASGFGTRIWNLSDRPVELQIRVGSILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGCVSVRKKPR  FC
Sbjct: 121 RIFRDHQRGCVSVRKKPRLGFC 142

[Close]
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match: A0A6J1I9A2 (LOW QUALITY PROTEIN: uncharacterized protein LOC111472255 OS=Cucurbita maxima OX=3661 GN=LOC111472255 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.4e-74
Identity = 134/142 (94.37%), Postives = 134/142 (94.37%), Query Frame = 0 

Query: 1   MPGFGVFPLGSCFDGCRDHAQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKR 60
           MPGFGVFPLGSCFDGCRDH Q SGFGTRIWN SDRPVELQIRVG ILKKVHSLKPGCSKR
Sbjct: 1   MPGFGVFPLGSCFDGCRDHTQASGFGTRIWNLSDRPVELQIRVGXILKKVHSLKPGCSKR 60

Query: 61  LKCKSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120
           LKCKSIYKAY PGRSM SLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI
Sbjct: 61  LKCKSIYKAYMPGRSMKSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEI 120

Query: 121 RIFRDHQRGCVSVRKKPRPDFC 143
           RIFRDHQRGCVSVRKKPR  FC
Sbjct: 121 RIFRDHQRGCVSVRKKPRLGFC 142

[Close]
BLAST of Sed0011127 vs. TAIR 10
Match: AT3G06145.1 (unknown protein; Has 15 Blast hits to 15 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 15; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 156.4 bits (394), Expect = 1.7e-38
Identity = 76/135 (56.30%), Postives = 98/135 (72.59%), Query Frame = 0 

Query: 9   LGSCFDGCRDH-----AQGSGFGTRIWNSSDRPVELQIRVGSILKKVHSLKPGCSKRLKC 68
           L  C+ GC D         +   TR WN SDRPVELQIRVGSILK+VH+LKPG SK+L+ 
Sbjct: 28  LRQCYGGCGDRTHLLPCSIATTSTRFWNLSDRPVELQIRVGSILKRVHTLKPGRSKKLRH 87

Query: 69  KSIYKAYKPGRSMNSLLYYYDEACQPYVWIHESGGDSMRMVKQQFISLEDLREFSEIRIF 128
           +SI++AY   +     L YYD+ C PYVW+HE+G D  +MVKQQ++SLEDLR++SEIR+F
Sbjct: 88  RSIHRAYVDDQEGRRWL-YYDDTCLPYVWVHETGTDLSKMVKQQYVSLEDLRDYSEIRVF 147

Query: 129 RDHQRGCVSVRKKPR 139
           +D QRGCV+V K+ R
Sbjct: 148 KDLQRGCVAVEKRDR 161

The following BLAST results are available for this feature:
BLAST of Sed0011127 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008438723.14.4e-7694.37PREDICTED: uncharacterized protein LOC103483746 [Cucumis melo] >KAA0049398.1 unc... [more]
XP_011650993.12.8e-7594.37uncharacterized protein LOC105434839 [Cucumis sativus] >KGN57027.1 hypothetical ... [more]
XP_038875149.13.7e-7594.37uncharacterized protein LOC120067683 [Benincasa hispida][more]
XP_022936946.18.3e-7595.77uncharacterized protein LOC111443378 [Cucurbita moschata] >KAG6597467.1 hypothet... [more]
XP_023522910.11.1e-7495.07uncharacterized protein LOC111786960 [Cucurbita pepo subsp. pepo][more]
BLAST of Sed0011127 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Sed0011127 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7U2C92.1e-7694.37Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3AWR32.1e-7694.37uncharacterized protein LOC103483746 OS=Cucumis melo OX=3656 GN=LOC103483746 PE=... [more]
A0A0A0L8V01.4e-7594.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150060 PE=4 SV=1[more]
A0A6J1F8W24.0e-7595.77uncharacterized protein LOC111443378 OS=Cucurbita moschata OX=3662 GN=LOC1114433... [more]
A0A6J1I9A24.4e-7494.37LOW QUALITY PROTEIN: uncharacterized protein LOC111472255 OS=Cucurbita maxima OX... [more]
BLAST of Sed0011127 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G06145.11.7e-3856.30unknown protein; Has 15 Blast hits to 15 proteins in 7 species: Archae - 0; Bact... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0011127.1Sed0011127.1mRNA