Sed0010801 (gene) Chayote v1

Overview
NameSed0010801
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG07: 4262324 .. 4266358 (+)
RNA-Seq ExpressionSed0010801
SyntenySed0010801
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAAAATAAATAAGATATAAAGCAATGTTCATAAATCATGACTATTTAATACCAAATAATGGGGATTGATCATCCACACACATTCAAATTTCTTCATCTTCCTCCTTCAAATGTCTCTGTTTCAGACAATGTACGGCCTCAAAAACAGAGCTCAAATCACTTCAAATCCCACCTCCATTTCCGCCATTGTCACCTTCTCCTTCTTCTTCATCCTCTTCTCTTCAAAACCCATTTCTTCAAATCCCCAAATCATTCACAATCAGCAAACCCCTCTCTCCCTCTGCAATGGCACTCTGTTTCCAGACCTCTGTTTCTCCAAACTCTCCTCTTTCCCACAATTGTCTTCCCTCTCGCCGGAAAAACTCGCCGGCGCCGCCCTCAACTTCACCTCCGACGAATTCCACCGCGCCTACGCCAACTGCACCAAGCTCAAGTCCCTCCTCTACGGCAGCCTGAACCCGACCGACCGCCACGCCCTCGACGACTGCCTCGAGCTTCTCGACGGCAGCATTGCCGAGCTCCAAGCTTCCATTTCCGATCTGGCGCCGTCGGAGTGCGCCGCCAGCCACTCCCACGACCTGTTGACTTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGCATCGACGGATTTTACAACAGCTCTGGGGATGTGAGGAGCCGGGTGGGGATGTATTTTGGGGAAAAACTTGTGCGGCATTTGAGCATTGATTTGGCGATGTTGAAGAGAATCCCTGGAGTTGATCGGGCGGCGGCGGAGGTGTTTCCGGAGTATGGGGCGGTGAGCGGTGGGTTTCCGACGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACCAAGTTTGATTTGGTGGTGGCCAAGGACGGCTCCGGGAATTTCACCACCGTCGGTGAGGCCGTCGCCGCCGCTCCGAACTCCAGCGCCACCAGGTAGGTTTGTTTAACTTAACTTTTTATTTGTTTATTCATTTATGTTTTTTTTTAATTATTATTATTCACTGAGATTGTTTGCAATAAATTAAATTTTCATTACCAGGTTAATAGTTTAGAAATTACACATGTCCGTAAATTTGGCATCACATGCCATCACAGTTTCACATGTCTATTATTATTTTTATATAATATAGATTAATAATATAGCAATGAAATTTAATTAAAGAGGAAATACAATATAATTTCTTTTTGAAACATTCAATCACATTTTATTGAAATATGTTGATGGAAGATATTTTAGTACCTAGGGCTTTGCATAGTATCAGCATTCTTGTTTCAAAAGAAATCAGAAATCTTTGGTGCTAAGGAAATACTTTCCCATAAAACAAGTCTTGGTTGAGACTTACGTAACACCATCCGGTATCACATTAAAAATGTGAGTCTTCAAAATTTTACTGTAATTAGTTTTAAGATGAGTAACTTAAATATCATTTATAAATGTTAAATACTTCTGGCATTTCTAATGTACAAAAACAATTCAGAAAGTGTATATTTATTAAAAGAAAAATGTTCATCTGGAGTAATATTAGCCAATCTGTAAAAAAAATGTAATTAAAATATAATATAATGGTAACTAGAACAATGACTTAATTGATATAGATGTGTGTCACTCAAAAAGAAAAATAAAAAATAAAAAATCTTGATTTATATTAAGTTGCTGAACTCGAAAAAGTAGAATTAATGATAAACTCGAATTTGTTATATATATAGGCATGGCAAGGATTAAGTTTGATAATCCAAAGATTTGGAAATGGTTAATGATGGAAAAAAATTGTGATTTACGAATGGCAGGTTCGTAATATACATAAAAGCAGGGGCGTATTTTGAAAACGTGGAAATAGAAAGGAAAAAGACGAACTTGATGTTCGTCGGCGACGGGATCGGGAAAACGCTCATAAAGGGCGACCGGAACGTCGTCGACGGCTGGACCACTTTCCGATCAGCCACTGTCGGTCAGTTTTCTTCTCTCTTTTTGTCATGATGAATTAATATTTTTATTTTTTAAATGAGATTATTGTATTTTTTTTAATGTGAAGTATTCGTTAATTTACAATTTAATTTGAATGATTGTTGAAATATTATTGAAATAGTTAGTGTTATTAGGAGTGTCCTCACCTACAGACCATAATTTCTAATTCTCCTTTTTTTCTTATTATTAATAAACTTTAAAAACCCGTACAAAATAGGAAGGAAAAAAAAAAGTAGTAATCTTTTCTTTTCTTTTTTTTTGAAAAAGTAGTAAATCATAAAAGTTGAATCTGAAATGTGAACTAAAAAACGTTAGAATCTGAAATTTAAATTAAAATAGAAAATGATTTAAATTTTAAAATAAGGAAATGATAAAGTACTGCAAGGCGTCAAATACGATCAAGCCGCGTGTCCATTGGATAAGATTGCTATATCTGTCGGCCTTTTCTTGTCTCCTCTTTTTTCGATCAAATCAATTTTTTTTTTTTGACAAATATTAGAAGAAGTTTGAGAAATTTGGAAGAATTTAAATGGTGTATTCATTAATTCTTCTTTAGGCGATTTAAATTAATTACTCATTTTTATAATAAAAAGTAAAATTTTTATTTTTTACCATTTATTTCTAAATATTAATTTATAATTATTTTAATATAATTTTTCATTTTTAGTTAGCTAAACTTTTTTTTAATAAATTAAAATAATTCATTTTTAATCATTCAAAATAATGATTCATTTCAAATTTACATTTAAAGAAGCGTAGTCACACCATCAATCCTTTCACTAATTTCGTATTGGAGTTTCTACTAACTAGCTCAGTCAGTGCCATTTTAGATTTCAAAAAGAAATAAATAGTGTTATTTTATGTTAAAATATATAAACCAATGGTCTAACCGTTTTGCTAATATTAAATTTTCATTTAATTTTGAAAACTCTAGTCTAAAATGAATGAAGTTCGAATGGTTTTAAATTCATTATTCCTCTGACATCTTTAACTTTCGATCCTAAAAATTTTCTAAAAACTGAACATGGTTTATTATGTGCAGCGGCGGTTGGCCCCGGGTTCATAGCCAAAGGCATAACATTTGAAAACTACGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTTCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTTATCGGCTATCAAGACACGCTCTACGTCCACTCCCTCCGCCAATTCTACCGCGACTGCGACGTCTACGGTACCATCGACTTTATCTTCGGCAATGCTGCTGTCGTTTTCCAAAATTGCAACCTCTACGCCCGAAAACCCAACTCGAACCAACGCAACATATTCACTGCCCAAGGTCGTGATGACCCGAACCAGAACACTGGAATCTCTATCTTGAATTGTAAGGTTGAAGCTGCCTCTGATTTGATTCCGGTTCTTTCAACGTTCAAGACTTACTTGGGCCGGCCTTGGAAGCTTTACTCGAGGACGGTTTTTCTTCGGTCGTTTATTGGGCAGTTGGTCGACCCAGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCATTGGATACTTTGTACTATGGGGAGTATTTGAACCGGGGGCCTGGATCGAATACGACTGCTCGAGTTACATGGCCGGGTTATCGGGTGATTACTAACTCGACAGAGGCAAGCCAGTTTACCGTGGATGCGTTTATACAGGGAAGCTCGTGGCTCGATTCGACCGGGATTCCGTTCTTCTCCGGTTTGGTTTGATTGGTGAACCGGATCTAGGAGATGTTGAGATTTGAGGTTTCTCCTTGTATTTTGGTAATGATTAATTATGATGCTGTAATGTTGAACCGGCTGGGAGTGAAATGGCATATTCACGGTCCAGAAAAGGACCGGTTTGTGATCATATATATTTTTTTTTTTTTTTTGAAAGAAAACCAAATATCATTCACCAACCACCAAACCCATACAATCATTCTCCAACTCGGAGCCAACAACAGGGGGGAGATTCCCCCTCCAAAAATGAGACCTATCCTCAACAGAGAGGGTAGGATCCTTCCAACAAAAGTAATCTAAATCGCA

mRNA sequence

TAGAAAATAAATAAGATATAAAGCAATGTTCATAAATCATGACTATTTAATACCAAATAATGGGGATTGATCATCCACACACATTCAAATTTCTTCATCTTCCTCCTTCAAATGTCTCTGTTTCAGACAATGTACGGCCTCAAAAACAGAGCTCAAATCACTTCAAATCCCACCTCCATTTCCGCCATTGTCACCTTCTCCTTCTTCTTCATCCTCTTCTCTTCAAAACCCATTTCTTCAAATCCCCAAATCATTCACAATCAGCAAACCCCTCTCTCCCTCTGCAATGGCACTCTGTTTCCAGACCTCTGTTTCTCCAAACTCTCCTCTTTCCCACAATTGTCTTCCCTCTCGCCGGAAAAACTCGCCGGCGCCGCCCTCAACTTCACCTCCGACGAATTCCACCGCGCCTACGCCAACTGCACCAAGCTCAAGTCCCTCCTCTACGGCAGCCTGAACCCGACCGACCGCCACGCCCTCGACGACTGCCTCGAGCTTCTCGACGGCAGCATTGCCGAGCTCCAAGCTTCCATTTCCGATCTGGCGCCGTCGGAGTGCGCCGCCAGCCACTCCCACGACCTGTTGACTTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGCATCGACGGATTTTACAACAGCTCTGGGGATGTGAGGAGCCGGGTGGGGATGTATTTTGGGGAAAAACTTGTGCGGCATTTGAGCATTGATTTGGCGATGTTGAAGAGAATCCCTGGAGTTGATCGGGCGGCGGCGGAGGTGTTTCCGGAGTATGGGGCGGTGAGCGGTGGGTTTCCGACGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACCAAGTTTGATTTGGTGGTGGCCAAGGACGGCTCCGGGAATTTCACCACCGTCGGTGAGGCCGTCGCCGCCGCTCCGAACTCCAGCGCCACCAGGTTCGTAATATACATAAAAGCAGGGGCGTATTTTGAAAACGTGGAAATAGAAAGGAAAAAGACGAACTTGATGTTCGTCGGCGACGGGATCGGGAAAACGCTCATAAAGGGCGACCGGAACGTCGTCGACGGCTGGACCACTTTCCGATCAGCCACTGTCGCGGCGGTTGGCCCCGGGTTCATAGCCAAAGGCATAACATTTGAAAACTACGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTTCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTTATCGGCTATCAAGACACGCTCTACGTCCACTCCCTCCGCCAATTCTACCGCGACTGCGACGTCTACGGTACCATCGACTTTATCTTCGGCAATGCTGCTGTCGTTTTCCAAAATTGCAACCTCTACGCCCGAAAACCCAACTCGAACCAACGCAACATATTCACTGCCCAAGGTCGTGATGACCCGAACCAGAACACTGGAATCTCTATCTTGAATTGTAAGGTTGAAGCTGCCTCTGATTTGATTCCGGTTCTTTCAACGTTCAAGACTTACTTGGGCCGGCCTTGGAAGCTTTACTCGAGGACGGTTTTTCTTCGGTCGTTTATTGGGCAGTTGGTCGACCCAGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCATTGGATACTTTGTACTATGGGGAGTATTTGAACCGGGGGCCTGGATCGAATACGACTGCTCGAGTTACATGGCCGGGTTATCGGGTGATTACTAACTCGACAGAGGCAAGCCAGTTTACCGTGGATGCGTTTATACAGGGAAGCTCGTGGCTCGATTCGACCGGGATTCCGTTCTTCTCCGGTTTGGTTTGATTGGTGAACCGGATCTAGGAGATGTTGAGATTTGAGGTTTCTCCTTGTATTTTGGTAATGATTAATTATGATGCTGTAATGTTGAACCGGCTGGGAGTGAAATGGCATATTCACGGTCCAGAAAAGGACCGGTTTGTGATCATATATATTTTTTTTTTTTTTTTGAAAGAAAACCAAATATCATTCACCAACCACCAAACCCATACAATCATTCTCCAACTCGGAGCCAACAACAGGGGGGAGATTCCCCCTCCAAAAATGAGACCTATCCTCAACAGAGAGGGTAGGATCCTTCCAACAAAAGTAATCTAAATCGCA

Coding sequence (CDS)

ATGTCTCTGTTTCAGACAATGTACGGCCTCAAAAACAGAGCTCAAATCACTTCAAATCCCACCTCCATTTCCGCCATTGTCACCTTCTCCTTCTTCTTCATCCTCTTCTCTTCAAAACCCATTTCTTCAAATCCCCAAATCATTCACAATCAGCAAACCCCTCTCTCCCTCTGCAATGGCACTCTGTTTCCAGACCTCTGTTTCTCCAAACTCTCCTCTTTCCCACAATTGTCTTCCCTCTCGCCGGAAAAACTCGCCGGCGCCGCCCTCAACTTCACCTCCGACGAATTCCACCGCGCCTACGCCAACTGCACCAAGCTCAAGTCCCTCCTCTACGGCAGCCTGAACCCGACCGACCGCCACGCCCTCGACGACTGCCTCGAGCTTCTCGACGGCAGCATTGCCGAGCTCCAAGCTTCCATTTCCGATCTGGCGCCGTCGGAGTGCGCCGCCAGCCACTCCCACGACCTGTTGACTTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGCATCGACGGATTTTACAACAGCTCTGGGGATGTGAGGAGCCGGGTGGGGATGTATTTTGGGGAAAAACTTGTGCGGCATTTGAGCATTGATTTGGCGATGTTGAAGAGAATCCCTGGAGTTGATCGGGCGGCGGCGGAGGTGTTTCCGGAGTATGGGGCGGTGAGCGGTGGGTTTCCGACGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACCAAGTTTGATTTGGTGGTGGCCAAGGACGGCTCCGGGAATTTCACCACCGTCGGTGAGGCCGTCGCCGCCGCTCCGAACTCCAGCGCCACCAGGTTCGTAATATACATAAAAGCAGGGGCGTATTTTGAAAACGTGGAAATAGAAAGGAAAAAGACGAACTTGATGTTCGTCGGCGACGGGATCGGGAAAACGCTCATAAAGGGCGACCGGAACGTCGTCGACGGCTGGACCACTTTCCGATCAGCCACTGTCGCGGCGGTTGGCCCCGGGTTCATAGCCAAAGGCATAACATTTGAAAACTACGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTTCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTTATCGGCTATCAAGACACGCTCTACGTCCACTCCCTCCGCCAATTCTACCGCGACTGCGACGTCTACGGTACCATCGACTTTATCTTCGGCAATGCTGCTGTCGTTTTCCAAAATTGCAACCTCTACGCCCGAAAACCCAACTCGAACCAACGCAACATATTCACTGCCCAAGGTCGTGATGACCCGAACCAGAACACTGGAATCTCTATCTTGAATTGTAAGGTTGAAGCTGCCTCTGATTTGATTCCGGTTCTTTCAACGTTCAAGACTTACTTGGGCCGGCCTTGGAAGCTTTACTCGAGGACGGTTTTTCTTCGGTCGTTTATTGGGCAGTTGGTCGACCCAGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCATTGGATACTTTGTACTATGGGGAGTATTTGAACCGGGGGCCTGGATCGAATACGACTGCTCGAGTTACATGGCCGGGTTATCGGGTGATTACTAACTCGACAGAGGCAAGCCAGTTTACCGTGGATGCGTTTATACAGGGAAGCTCGTGGCTCGATTCGACCGGGATTCCGTTCTTCTCCGGTTTGGTTTGA

Protein sequence

MSLFQTMYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDLCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDCLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRVGMYFGEKLVRHLSIDLAMLKRIPGVDRAAAEVFPEYGAVSGGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAFIQGSSWLDSTGIPFFSGLV
Homology
BLAST of Sed0010801 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 979.5 bits (2531), Expect = 1.2e-281
Identity = 488/558 (87.46%), Postives = 514/558 (92.11%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDL 66
           MYGLKNRAQI SNPTSIS I TF F F  FS KPISSNPQI    QT  SLCNGTLFP L
Sbjct: 1   MYGLKNRAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQI---HQTARSLCNGTLFPHL 60

Query: 67  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 126
           CFSKLSSFPQL+SLSPEKLAG+A+NFT+ E   AYANCTKLK++LYG LNPTDR ALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDC 120

Query: 127 LELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRV 186
           LELLD SIAELQASI DLAP + AA HSHDLLTL+SAAMTNHRTC+DGFYNSSG VRSRV
Sbjct: 121 LELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 187 GMYFGEKLVRHLSIDLAMLKRIPGVDR--AAAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 246
            +Y G K+ +HLSIDLAMLK+IPGV+R  AA EV PEYGAV GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLG-KIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAA 240

Query: 247 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 306
           VNETKF+LVVAKDGSGNFTTVGEAVAAAPNSS TRFVIYIKAGAYFENVEIER+KTNLMF
Sbjct: 241 VNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMF 300

Query: 307 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 366
           VGDGIGKTLIK DRNVVDGWTTFRSATVA VG GFIAKGITFENYAGPSKHQAVA RSNS
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 367 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 426
           DFSAFYQCSF+GYQDTLYVHSLRQFYRDCD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 427 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 486
           RNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKLYSRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480

Query: 487 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 546
           QLVDPAGWLEWNGTFALDTLYYGEY+NRGPGSNTT RVTWPGYRVITN++EASQFTV+ F
Sbjct: 481 QLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGF 540

Query: 547 IQGSSWLDSTGIPFFSGL 563
           IQGSSWL+STGIPFFSGL
Sbjct: 541 IQGSSWLNSTGIPFFSGL 554

BLAST of Sed0010801 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 967.6 bits (2500), Expect = 4.6e-278
Identity = 486/559 (86.94%), Postives = 513/559 (91.77%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPT-SISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPD 66
           MYGL++RAQ +SNPT SISAI T      LFS KPISSNPQ     QT  SLCNGTLFP+
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQ---THQTARSLCNGTLFPN 60

Query: 67  LCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDD 126
           LCFSKLSSFPQL+SLSPEKLAG+ALNFT+DE H AYANC+KLK+L YG LNPTDR ALDD
Sbjct: 61  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDD 120

Query: 127 CLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSR 186
           CLELLDGSIAELQ SI DL+PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR R
Sbjct: 121 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 180

Query: 187 VGMYFGEKLVRHLSIDLAMLKRIPGVDRAAA--EVFPEYGAVSGGFPTWVSVKDRRLLQA 246
           V +Y G K+ +HLSIDLAMLK+IPGV RAA   EV PEYGAV GGFP WVSVKDRRLLQA
Sbjct: 181 VELYLG-KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 240

Query: 247 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLM 306
           AVNETKF+LVVAKDGSGNFTTVGEAVAAAPNSS TRFVIYIKAGAYFENVEIERKKTNLM
Sbjct: 241 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 300

Query: 307 FVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSN 366
           FVGDGIGKTLIK DRNVVDGWTTFRSATVAAVG GFIAKGITFEN+AGPSKHQAVAFRSN
Sbjct: 301 FVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSN 360

Query: 367 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 426
           SDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYARKPNSN
Sbjct: 361 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 420

Query: 427 QRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFI 486
           QRNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKLYSRT+FL+SFI
Sbjct: 421 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFI 480

Query: 487 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDA 546
           GQLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNSTEASQFTV+A
Sbjct: 481 GQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 540

Query: 547 FIQGSSWLDSTGIPFFSGL 563
           FIQGS WL+ST IPFFSGL
Sbjct: 541 FIQGSEWLNSTEIPFFSGL 555

BLAST of Sed0010801 vs. NCBI nr
Match: XP_008451545.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 967.2 bits (2499), Expect = 6.0e-278
Identity = 485/558 (86.92%), Postives = 512/558 (91.76%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDL 66
           MYGL+NRAQI+SNPTSIS I TF F F LFSSKPISSNPQI    QT  SLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQI---HQTARSLCNGTLFPNL 60

Query: 67  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 126
           CFSKLSSFPQL+SLSPEKLAG+ALNFT+ E   AYANCT LK+ LY  LNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDC 120

Query: 127 LELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRV 186
           LELLD SIAEL+ASI DL PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VRSRV
Sbjct: 121 LELLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 187 GMYFGEKLVRHLSIDLAMLKRIPGVDRA--AAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 246
            +Y G K+ +HLSIDLAMLK+IPGV+RA    EV PEYGAV GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLG-KIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 247 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 306
           VNETKF+LVVAKDGSGNFTTV EAVAAAPNSS TRFVIYIKAGAYFENVEI R K+NLMF
Sbjct: 241 VNETKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 307 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 366
           VGDGIGKTLIKGDRNVVDGWTTFRSATVA VG GFIAKGITFENYAGPSKHQAVA RSNS
Sbjct: 301 VGDGIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 367 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 426
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 427 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 486
           RNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKL+SRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIG 480

Query: 487 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 546
           QLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTV+ F
Sbjct: 481 QLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEF 540

Query: 547 IQGSSWLDSTGIPFFSGL 563
           IQGSSWL+STGIPFFSGL
Sbjct: 541 IQGSSWLNSTGIPFFSGL 554

BLAST of Sed0010801 vs. NCBI nr
Match: KAA0046658.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 966.8 bits (2498), Expect = 7.9e-278
Identity = 484/558 (86.74%), Postives = 512/558 (91.76%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDL 66
           MYGL+NRAQI+SNPTSIS I TF F F LFSSKPISSNPQI    QT  SLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQI---HQTARSLCNGTLFPNL 60

Query: 67  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 126
           CFSKLSSFPQL+SLSPEKLAG+ALNFT+ E   AYANCT LK+ LY  LNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDC 120

Query: 127 LELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRV 186
           LELLD SIAEL+ASI DL PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VRSRV
Sbjct: 121 LELLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 187 GMYFGEKLVRHLSIDLAMLKRIPGVDRA--AAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 246
            +Y G K+ +HLSIDLAMLK+IPGV+RA    EV PEYGAV GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLG-KIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 247 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 306
           VNETKF+LVVAKDGSGNFTTV EAVAAAPNSS TRFVIYIKAGAYFENVEI R K+NLMF
Sbjct: 241 VNETKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 307 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 366
           VGDGIGKTLIKGDRNVVDGWTTFRSATVA +G GFIAKGITFENYAGPSKHQAVA RSNS
Sbjct: 301 VGDGIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 367 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 426
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 427 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 486
           RNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKL+SRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIG 480

Query: 487 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 546
           QLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTV+ F
Sbjct: 481 QLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEF 540

Query: 547 IQGSSWLDSTGIPFFSGL 563
           IQGSSWL+STGIPFFSGL
Sbjct: 541 IQGSSWLNSTGIPFFSGL 554

BLAST of Sed0010801 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.1 bits (2483), Expect = 4.3e-276
Identity = 483/559 (86.40%), Postives = 513/559 (91.77%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPT-SISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPD 66
           MYGL+NRAQ +SNPT SISAI TF    IL S KPISSNPQ     QT  SLCNGTLFP+
Sbjct: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQ---THQTARSLCNGTLFPN 60

Query: 67  LCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDD 126
           LCFSKLSSFPQL+SLSPEKLAG+ALNFT+DE H AYANC+KLK+L YG L+PTDR ALDD
Sbjct: 61  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDD 120

Query: 127 CLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSR 186
           CLELLDGSIAELQ SI DL+PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR R
Sbjct: 121 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 180

Query: 187 VGMYFGEKLVRHLSIDLAMLKRIPGVDRAAA--EVFPEYGAVSGGFPTWVSVKDRRLLQA 246
           V +Y G K+ +HLSIDLAMLK+IPGV RAA   EV PEYGAV GGFP WVSVKDRRLLQA
Sbjct: 181 VELYLG-KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 240

Query: 247 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLM 306
           AVNETKF+LVVAKDGSGNFTTVGEAVAAAPNSS TRFVIYIKAGAYFENVEIERKKTNL+
Sbjct: 241 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLV 300

Query: 307 FVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSN 366
           FVGDGIGKTLIK +RNVVDGWTTFRSATVAAVG GFIAKGITFEN+AGPSKHQAVAFRSN
Sbjct: 301 FVGDGIGKTLIKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSN 360

Query: 367 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 426
           SDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGT+DFIFGNAAVVFQNCNLYARKPNSN
Sbjct: 361 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSN 420

Query: 427 QRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFI 486
           QRNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVL +F+TYLGRPWKLYSRTVFL+SFI
Sbjct: 421 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFI 480

Query: 487 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDA 546
           GQLV+P GWLEW+GTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNSTEASQFTV+A
Sbjct: 481 GQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 540

Query: 547 FIQGSSWLDSTGIPFFSGL 563
           FIQGS WL+ST IPFFSGL
Sbjct: 541 FIQGSEWLNSTEIPFFSGL 555

BLAST of Sed0010801 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.5e-150
Identity = 281/518 (54.25%), Postives = 356/518 (68.73%), Query Frame = 0

Query: 55  LSLCNGTLFPDLCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGS 114
           LSL    LF    FS   +    SS    +L   ALN T    + + +N + L   L  +
Sbjct: 10  LSLTFLLLFLSTVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQRLGSN 69

Query: 115 LNPTDRHALDDCLELLDGSIAELQASISDLAPSECAASHS---HDLLTLVSAAMTNHRTC 174
           L+  D  A DDCLELLD ++ +L  +IS L       SHS   H++  L+SAAMTN RTC
Sbjct: 70  LSHRDLCAFDDCLELLDDTVFDLTTAISKL------RSHSPELHNVKMLLSAAMTNTRTC 129

Query: 175 IDGFYNSSGD--VRSRVGMYFGE---------KLVRHLSIDLAMLKRIPGVDRAAAEVFP 234
           +DGF +S  D  + +     +G           +  H+S  LAML+ IPG         P
Sbjct: 130 LDGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPG-------HIP 189

Query: 235 EYGAVSGGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRF 294
                  GFP WVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TRF
Sbjct: 190 GKVKEDVGFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRF 249

Query: 295 VIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFI 354
           VIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK +R+  DGWT F SATV   G GFI
Sbjct: 250 VIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFI 309

Query: 355 AKGITFENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 414
           AK ++F NYAGP KHQAVA RS+SD SA+Y+CSF  YQDT+YVHS +QFYR+CD+YGT+D
Sbjct: 310 AKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVD 369

Query: 415 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLS 474
           FIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGR++  + TGISI++ ++ AA DLIPV +
Sbjct: 370 FIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQA 429

Query: 475 TFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTA 534
            FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T 
Sbjct: 430 NFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTN 489

Query: 535 RVTWPGYRVITNSTEASQFTVDAFIQGSSWLDSTGIPF 559
           RV WPG++ I    EASQF+V  FI G+ WL+ST IPF
Sbjct: 490 RVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Sed0010801 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.3e-145
Identity = 251/319 (78.68%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 244 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLM 303
           AV +   D+VVAKDGSGNFTTVG AVAAA +SS  RFVIYIK GAYFE V++++KKTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 304 FVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSN 363
           F+GDGIGKT IKG+R+VVDGWTTFRS+TVA VG GFIA+GI+FENYAGPSKHQAVA RS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 364 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 423
           +DFSAFYQCSF+GYQDTLYVHSLRQFY +CDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 424 QRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFI 483
           Q+NIFTAQGRDDPNQNTGISILNCKV AA+DLIPVLS+FKTYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 484 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDA 543
             L+DPAGWLEW+G FAL TLYY EY N GPGSNTTARVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 544 FIQGSSWLDSTGIPFFSGL 563
           FIQGS WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Sed0010801 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 510.4 bits (1313), Expect = 2.6e-143
Identity = 281/572 (49.13%), Postives = 359/572 (62.76%), Query Frame = 0

Query: 11  KNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTP----LSLCNGTLFPDL 70
           KN  ++  +  +I+ ++  S   I  ++   + N +I     T      S+C+ TL+P+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 71  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 130
           CFS +++       S +++  A+LN T+      Y    KL +   G L P +  AL DC
Sbjct: 75  CFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG-LTPREVTALHDC 134

Query: 131 LELLDGSIAELQASISDL---APSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVR 190
           LE +D ++ EL  ++ DL      +    H+ DL TL+S+A+TN  TC+DGF     D +
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 191 SRVGMYFGEKLVRHL-SIDLAMLKRIPGVDRAAAEVFPEYGAV----------------S 250
            R  +  G+  V H+ S  LAM+K +   D A  E+  +                    S
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 254

Query: 251 GGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKA 310
            G+P W+SV DRRLLQ +    K D  VA DGSG+FTTV  AVAAAP  S  RFVI+IKA
Sbjct: 255 DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKA 314

Query: 311 GAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITF 370
           G Y ENVE+ +KKTN+MF+GDG GKT+I G RNVVDG TTF SATVAAVG  F+A+ ITF
Sbjct: 315 GVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITF 374

Query: 371 ENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNA 430
           +N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF+  C + GT+DFIFGNA
Sbjct: 375 QNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNA 434

Query: 431 AVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYL 490
           A V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++   SDL+ V  TF TYL
Sbjct: 435 AAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYL 494

Query: 491 GRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPG 550
           GRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y EYLNRG G+ T  RV W G
Sbjct: 495 GRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKG 554

Query: 551 YRVITNSTEASQFTVDAFIQGSSWLDSTGIPF 559
           Y+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 555 YKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Sed0010801 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.4e-143
Identity = 276/557 (49.55%), Postives = 358/557 (64.27%), Query Frame = 0

Query: 12  NRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDLCFSKL 71
           N   I   P  I   +    FF++F S  +SS        +T   L      P+L     
Sbjct: 3   NNHPIREKPKHIIFNLLSLIFFLIFLSTVVSSQSPSYTTHKTQ-RLTETKTIPELII--- 62

Query: 72  SSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDCLELLD 131
                           A LN T  + + A +N + L++ L+ +L   +R A +DCL LLD
Sbjct: 63  ----------------ADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLD 122

Query: 132 GSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRVGMYFG 191
            +I++L+ ++SDL  S   +   +D+  L++  MT   TC+DGF  S  +  + +     
Sbjct: 123 DTISDLETAVSDLRSS---SLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELP 182

Query: 192 EKL-------VRHLSIDLAMLKRIPGVDRAAAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 251
           E L         +LS  L ML+ I     +  +  P+   V   +P+W+S  D+RLL+A 
Sbjct: 183 ENLKEIILDISNNLSNSLHMLQVI-----SRKKPSPKSSEVDVEYPSWLSENDQRLLEAP 242

Query: 252 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 311
           V ET ++L VA DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF
Sbjct: 243 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 302

Query: 312 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 371
           +GDGIGKT+IK +R+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS S
Sbjct: 303 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 362

Query: 372 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 431
           D SAFY+C F GYQDTLYVHS +QFYR+CD+YGTIDFIFGNAAVVFQN +LYARKPN   
Sbjct: 363 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 422

Query: 432 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 491
           +  FTAQ R+  +Q TGISILNC++ AA DLIPV   FK YLGRPW+ YSRTV ++SFI 
Sbjct: 423 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 482

Query: 492 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 551
            L+ PAGWLE    FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  F
Sbjct: 483 DLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPF 531

Query: 552 IQGSSWLDSTGIPFFSG 562
           I GS+WL+STGIPF  G
Sbjct: 543 IDGSTWLNSTGIPFSLG 531

BLAST of Sed0010801 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.4e-138
Identity = 262/518 (50.58%), Postives = 337/518 (65.06%), Query Frame = 0

Query: 56  SLCNGTLFPDLCFSKLSSFPQLSS--LSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYG 115
           S C+ T +PDLCFS +++ P+ S    S + +   +LN T+      Y    KL  L   
Sbjct: 71  SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL--LKRT 130

Query: 116 SLNPTDRHALDDCLELLDGSIAELQASISDLAP---SECAASHSHDLLTLVSAAMTNHRT 175
           +L   ++ AL DCLE +D ++ EL  ++ DL      +  + H+ DL TL+SAAMTN  T
Sbjct: 131 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 190

Query: 176 CIDGFYNSSGDVRSRVGMYFGEKLVRHL-SIDLAMLKRIPGVDRAAAEV-----FPEYGA 235
           C+DGF +   +   R  +  G+  V  + S  LAM+K +   D             E  +
Sbjct: 191 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETS 250

Query: 236 VSGGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYI 295
              G+P W+S  DRRLLQ+  +    + VVA DGSGNF TV  AVAAAP     R++I I
Sbjct: 251 TVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRI 310

Query: 296 KAGAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGI 355
           KAG Y ENVE+ +K  N+MF+GDG  +T+I G RNVVDG TTF+SAT A VG GF+A+ I
Sbjct: 311 KAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDI 370

Query: 356 TFENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFG 415
           TF+N AGPSKHQAVA R  +D SAFY C  + YQDTLYVHS RQF+ +C + GT+DFIFG
Sbjct: 371 TFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFG 430

Query: 416 NAAVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKT 475
           NAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A SDL PV  +F T
Sbjct: 431 NAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPT 490

Query: 476 YLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTW 535
           YLGRPWK YSRTV ++S I  L+ PAGW EW+G FAL+TL+YGE+ N G G+ T+ RV W
Sbjct: 491 YLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKW 550

Query: 536 PGYRVITNSTEASQFTVDAFIQGSSWLDSTGIPFFSGL 563
            G+RVIT++TEA  FT  +FI GSSWL STG PF  GL
Sbjct: 551 KGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Sed0010801 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 486/559 (86.94%), Postives = 513/559 (91.77%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPT-SISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPD 66
           MYGL++RAQ +SNPT SISAI T      LFS KPISSNPQ     QT  SLCNGTLFP+
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQ---THQTARSLCNGTLFPN 60

Query: 67  LCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDD 126
           LCFSKLSSFPQL+SLSPEKLAG+ALNFT+DE H AYANC+KLK+L YG LNPTDR ALDD
Sbjct: 61  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDD 120

Query: 127 CLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSR 186
           CLELLDGSIAELQ SI DL+PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR R
Sbjct: 121 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 180

Query: 187 VGMYFGEKLVRHLSIDLAMLKRIPGVDRAAA--EVFPEYGAVSGGFPTWVSVKDRRLLQA 246
           V +Y G K+ +HLSIDLAMLK+IPGV RAA   EV PEYGAV GGFP WVSVKDRRLLQA
Sbjct: 181 VELYLG-KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 240

Query: 247 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLM 306
           AVNETKF+LVVAKDGSGNFTTVGEAVAAAPNSS TRFVIYIKAGAYFENVEIERKKTNLM
Sbjct: 241 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 300

Query: 307 FVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSN 366
           FVGDGIGKTLIK DRNVVDGWTTFRSATVAAVG GFIAKGITFEN+AGPSKHQAVAFRSN
Sbjct: 301 FVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSN 360

Query: 367 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 426
           SDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYARKPNSN
Sbjct: 361 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 420

Query: 427 QRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFI 486
           QRNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKLYSRT+FL+SFI
Sbjct: 421 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFI 480

Query: 487 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDA 546
           GQLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNSTEASQFTV+A
Sbjct: 481 GQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 540

Query: 547 FIQGSSWLDSTGIPFFSGL 563
           FIQGS WL+ST IPFFSGL
Sbjct: 541 FIQGSEWLNSTEIPFFSGL 555

BLAST of Sed0010801 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.9e-278
Identity = 485/558 (86.92%), Postives = 512/558 (91.76%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDL 66
           MYGL+NRAQI+SNPTSIS I TF F F LFSSKPISSNPQI    QT  SLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQI---HQTARSLCNGTLFPNL 60

Query: 67  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 126
           CFSKLSSFPQL+SLSPEKLAG+ALNFT+ E   AYANCT LK+ LY  LNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDC 120

Query: 127 LELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRV 186
           LELLD SIAEL+ASI DL PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VRSRV
Sbjct: 121 LELLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 187 GMYFGEKLVRHLSIDLAMLKRIPGVDRA--AAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 246
            +Y G K+ +HLSIDLAMLK+IPGV+RA    EV PEYGAV GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLG-KIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 247 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 306
           VNETKF+LVVAKDGSGNFTTV EAVAAAPNSS TRFVIYIKAGAYFENVEI R K+NLMF
Sbjct: 241 VNETKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 307 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 366
           VGDGIGKTLIKGDRNVVDGWTTFRSATVA VG GFIAKGITFENYAGPSKHQAVA RSNS
Sbjct: 301 VGDGIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 367 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 426
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 427 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 486
           RNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKL+SRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIG 480

Query: 487 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 546
           QLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTV+ F
Sbjct: 481 QLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEF 540

Query: 547 IQGSSWLDSTGIPFFSGL 563
           IQGSSWL+STGIPFFSGL
Sbjct: 541 IQGSSWLNSTGIPFFSGL 554

BLAST of Sed0010801 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.8e-278
Identity = 484/558 (86.74%), Postives = 512/558 (91.76%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDL 66
           MYGL+NRAQI+SNPTSIS I TF F F LFSSKPISSNPQI    QT  SLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQI---HQTARSLCNGTLFPNL 60

Query: 67  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 126
           CFSKLSSFPQL+SLSPEKLAG+ALNFT+ E   AYANCT LK+ LY  LNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDC 120

Query: 127 LELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRV 186
           LELLD SIAEL+ASI DL PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VRSRV
Sbjct: 121 LELLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 187 GMYFGEKLVRHLSIDLAMLKRIPGVDRA--AAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 246
            +Y G K+ +HLSIDLAMLK+IPGV+RA    EV PEYGAV GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLG-KIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 247 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 306
           VNETKF+LVVAKDGSGNFTTV EAVAAAPNSS TRFVIYIKAGAYFENVEI R K+NLMF
Sbjct: 241 VNETKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 307 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 366
           VGDGIGKTLIKGDRNVVDGWTTFRSATVA +G GFIAKGITFENYAGPSKHQAVA RSNS
Sbjct: 301 VGDGIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 367 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 426
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 427 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 486
           RNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGRPWKL+SRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIG 480

Query: 487 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 546
           QLV+P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTV+ F
Sbjct: 481 QLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEF 540

Query: 547 IQGSSWLDSTGIPFFSGL 563
           IQGSSWL+STGIPFFSGL
Sbjct: 541 IQGSSWLNSTGIPFFSGL 554

BLAST of Sed0010801 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 3.0e-275
Identity = 480/559 (85.87%), Postives = 510/559 (91.23%), Query Frame = 0

Query: 7   MYGLKNRAQITSNPT-SISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPD 66
           MYGL++RAQ +SNPT SISAI TF     L S KPISSNPQ     QT  SLCNGTLFP+
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQ---THQTARSLCNGTLFPN 60

Query: 67  LCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDD 126
           LCFSKLSSFPQL+SLSPEKLAG+ALNFT+DE H AYANC+KLK+L YG LNPTDR ALDD
Sbjct: 61  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDD 120

Query: 127 CLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSR 186
           CLELLDGSIAELQ SI DL+PS+ AA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR R
Sbjct: 121 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 180

Query: 187 VGMYFGEKLVRHLSIDLAMLKRIPGVDRAAA--EVFPEYGAVSGGFPTWVSVKDRRLLQA 246
           V +Y G K+ +HLSIDLAMLK+IPGV RAA   EV PEYGAV GGFP WVSVKDRRLLQA
Sbjct: 181 VELYLG-KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 240

Query: 247 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLM 306
           +VNETKF+LVVAKDGSGNFTTVG AVAAAPNSS TRFVIYIKAGAYFENVEIERKKTNLM
Sbjct: 241 SVNETKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 300

Query: 307 FVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSN 366
           FVGDGIGKTLIK DRNVVDGWTTFRSATV AVG GFIAKGITFEN+AGPSKHQAVAFRSN
Sbjct: 301 FVGDGIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSN 360

Query: 367 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 426
           SDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAV+FQNCNLYARKPNSN
Sbjct: 361 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSN 420

Query: 427 QRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFI 486
           QRNIFTAQGR+DPNQNTGISILNCKVEAASDL+PVL +F+TYLGRPWKLYSRTVFL+SFI
Sbjct: 421 QRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFI 480

Query: 487 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDA 546
           GQLV+P GWLEW+GTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNSTEASQFTV+A
Sbjct: 481 GQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 540

Query: 547 FIQGSSWLDSTGIPFFSGL 563
           FIQGS WL+ST IPFFSGL
Sbjct: 541 FIQGSEWLNSTEIPFFSGL 555

BLAST of Sed0010801 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.7e-260
Identity = 450/514 (87.55%), Postives = 474/514 (92.22%), Query Frame = 0

Query: 52  QTPLSLCNGTLFPDLCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLL 111
           QT  SLCNGTLFPDLCFSKLSSFPQL+SLSPEKLAG+ALNFTS E   AY NCT LK+ L
Sbjct: 99  QTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHL 158

Query: 112 YGSLNPTDRHALDDCLELLDGSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTC 171
           Y  LNPTDRHALDDCLELLDGSIAEL+ASI DLAPS+  A HSHDLLTLVSAAMTNHRTC
Sbjct: 159 YSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTC 218

Query: 172 IDGFYNSSGDVRSRVGMYFGEKLVRHLSIDLAMLKRIPGVDRAAA---EVFPEYGAVSGG 231
           +DGFYNSSG VRSRV +Y G K+ +HLSIDLAMLK+IPGV+RA     E+ PEYGAV GG
Sbjct: 219 VDGFYNSSGTVRSRVELYLG-KIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGG 278

Query: 232 FPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGA 291
           FP WVSVKDRRLLQAAVNETKF++VVAKDGSGNFTTV EAVAAAPN+S TRFVIYIKAGA
Sbjct: 279 FPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGA 338

Query: 292 YFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFEN 351
           YFENVEI R K+NLMFVGDGIGKTLIK DRNVVDGWTTFRSATVA VG GFIAKGITFEN
Sbjct: 339 YFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFEN 398

Query: 352 YAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAV 411
           YAGPSKHQAVA RSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAV
Sbjct: 399 YAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAV 458

Query: 412 VFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGR 471
           VFQNCNLYARKPNSNQRNIFTAQGR+DPNQNTGISILNCKVEAASDLIPVLS+F+TYLGR
Sbjct: 459 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 518

Query: 472 PWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYR 531
           PWKLYSRTVFLRSFIGQL++P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYR
Sbjct: 519 PWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYR 578

Query: 532 VITNSTEASQFTVDAFIQGSSWLDSTGIPFFSGL 563
           VITN+TEASQFTV+ FIQGSSWL+ST IPFFSGL
Sbjct: 579 VITNATEASQFTVERFIQGSSWLNSTEIPFFSGL 611

BLAST of Sed0010801 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 531.9 bits (1369), Expect = 6.0e-151
Identity = 281/518 (54.25%), Postives = 356/518 (68.73%), Query Frame = 0

Query: 55  LSLCNGTLFPDLCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGS 114
           LSL    LF    FS   +    SS    +L   ALN T    + + +N + L   L  +
Sbjct: 10  LSLTFLLLFLSTVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQRLGSN 69

Query: 115 LNPTDRHALDDCLELLDGSIAELQASISDLAPSECAASHS---HDLLTLVSAAMTNHRTC 174
           L+  D  A DDCLELLD ++ +L  +IS L       SHS   H++  L+SAAMTN RTC
Sbjct: 70  LSHRDLCAFDDCLELLDDTVFDLTTAISKL------RSHSPELHNVKMLLSAAMTNTRTC 129

Query: 175 IDGFYNSSGD--VRSRVGMYFGE---------KLVRHLSIDLAMLKRIPGVDRAAAEVFP 234
           +DGF +S  D  + +     +G           +  H+S  LAML+ IPG         P
Sbjct: 130 LDGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPG-------HIP 189

Query: 235 EYGAVSGGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRF 294
                  GFP WVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TRF
Sbjct: 190 GKVKEDVGFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRF 249

Query: 295 VIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFI 354
           VIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK +R+  DGWT F SATV   G GFI
Sbjct: 250 VIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFI 309

Query: 355 AKGITFENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 414
           AK ++F NYAGP KHQAVA RS+SD SA+Y+CSF  YQDT+YVHS +QFYR+CD+YGT+D
Sbjct: 310 AKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVD 369

Query: 415 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLS 474
           FIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGR++  + TGISI++ ++ AA DLIPV +
Sbjct: 370 FIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQA 429

Query: 475 TFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTA 534
            FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T 
Sbjct: 430 NFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTN 489

Query: 535 RVTWPGYRVITNSTEASQFTVDAFIQGSSWLDSTGIPF 559
           RV WPG++ I    EASQF+V  FI G+ WL+ST IPF
Sbjct: 490 RVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Sed0010801 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 510.4 bits (1313), Expect = 1.9e-144
Identity = 281/572 (49.13%), Postives = 359/572 (62.76%), Query Frame = 0

Query: 11  KNRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTP----LSLCNGTLFPDL 70
           KN  ++  +  +I+ ++  S   I  ++   + N +I     T      S+C+ TL+P+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 71  CFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDC 130
           CFS +++       S +++  A+LN T+      Y    KL +   G L P +  AL DC
Sbjct: 75  CFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG-LTPREVTALHDC 134

Query: 131 LELLDGSIAELQASISDL---APSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVR 190
           LE +D ++ EL  ++ DL      +    H+ DL TL+S+A+TN  TC+DGF     D +
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 191 SRVGMYFGEKLVRHL-SIDLAMLKRIPGVDRAAAEVFPEYGAV----------------S 250
            R  +  G+  V H+ S  LAM+K +   D A  E+  +                    S
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 254

Query: 251 GGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKA 310
            G+P W+SV DRRLLQ +    K D  VA DGSG+FTTV  AVAAAP  S  RFVI+IKA
Sbjct: 255 DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKA 314

Query: 311 GAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITF 370
           G Y ENVE+ +KKTN+MF+GDG GKT+I G RNVVDG TTF SATVAAVG  F+A+ ITF
Sbjct: 315 GVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITF 374

Query: 371 ENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNA 430
           +N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF+  C + GT+DFIFGNA
Sbjct: 375 QNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNA 434

Query: 431 AVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYL 490
           A V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++   SDL+ V  TF TYL
Sbjct: 435 AAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYL 494

Query: 491 GRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPG 550
           GRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y EYLNRG G+ T  RV W G
Sbjct: 495 GRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKG 554

Query: 551 YRVITNSTEASQFTVDAFIQGSSWLDSTGIPF 559
           Y+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 555 YKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Sed0010801 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 510.0 bits (1312), Expect = 2.4e-144
Identity = 276/557 (49.55%), Postives = 358/557 (64.27%), Query Frame = 0

Query: 12  NRAQITSNPTSISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLSLCNGTLFPDLCFSKL 71
           N   I   P  I   +    FF++F S  +SS        +T   L      P+L     
Sbjct: 3   NNHPIREKPKHIIFNLLSLIFFLIFLSTVVSSQSPSYTTHKTQ-RLTETKTIPELII--- 62

Query: 72  SSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSLNPTDRHALDDCLELLD 131
                           A LN T  + + A +N + L++ L+ +L   +R A +DCL LLD
Sbjct: 63  ----------------ADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLD 122

Query: 132 GSIAELQASISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGDVRSRVGMYFG 191
            +I++L+ ++SDL  S   +   +D+  L++  MT   TC+DGF  S  +  + +     
Sbjct: 123 DTISDLETAVSDLRSS---SLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELP 182

Query: 192 EKL-------VRHLSIDLAMLKRIPGVDRAAAEVFPEYGAVSGGFPTWVSVKDRRLLQAA 251
           E L         +LS  L ML+ I     +  +  P+   V   +P+W+S  D+RLL+A 
Sbjct: 183 ENLKEIILDISNNLSNSLHMLQVI-----SRKKPSPKSSEVDVEYPSWLSENDQRLLEAP 242

Query: 252 VNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMF 311
           V ET ++L VA DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF
Sbjct: 243 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 302

Query: 312 VGDGIGKTLIKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNS 371
           +GDGIGKT+IK +R+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS S
Sbjct: 303 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 362

Query: 372 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 431
           D SAFY+C F GYQDTLYVHS +QFYR+CD+YGTIDFIFGNAAVVFQN +LYARKPN   
Sbjct: 363 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 422

Query: 432 RNIFTAQGRDDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIG 491
           +  FTAQ R+  +Q TGISILNC++ AA DLIPV   FK YLGRPW+ YSRTV ++SFI 
Sbjct: 423 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 482

Query: 492 QLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAF 551
            L+ PAGWLE    FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  F
Sbjct: 483 DLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPF 531

Query: 552 IQGSSWLDSTGIPFFSG 562
           I GS+WL+STGIPF  G
Sbjct: 543 IDGSTWLNSTGIPFSLG 531

BLAST of Sed0010801 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 483.8 bits (1244), Expect = 1.9e-136
Identity = 259/549 (47.18%), Postives = 343/549 (62.48%), Query Frame = 0

Query: 23  ISAIVTFSFFFILFSSKPISSNPQIIHNQQTPLS-LCNGTLFPDLCFSKLSSFPQLSSLS 82
           +  +V F  F  + +     + P++       +S  C+ +L+P+LC   L  FP   +  
Sbjct: 43  VVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTAD 102

Query: 83  PEKLAGAALNFTSDEFHRA-YANCTKLKSLLYGSLNPTDRHALDDCLELLDGSIAELQAS 142
             +L   + N T  +F +A Y + T    + Y  + P  R A D CLELLD S+  L  +
Sbjct: 103 ENELIHISFNATLQKFSKALYTSST----ITYTQMPPRVRSAYDSCLELLDDSVDALTRA 162

Query: 143 ISDLAPSECAASHSHDLLTLVSAAMTNHRTCIDGF---YNSSGDVRSRV---GMYFGEKL 202
           +S +       SHS D++T +S+AMTNH TC DGF       G+V+ +V        E +
Sbjct: 163 LSSVVVVSGDESHS-DVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMV 222

Query: 203 VRHLSIDLAMLKRIPGVDRAAAEVFPEYGAVSGGFPTWVSVKDRRLLQAAVNETKFDLVV 262
              L+I    +K + GV                  P W+  +DR LL    +  + D+ V
Sbjct: 223 SNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE-ELPNWLKREDRELLGTPTSAIQADITV 282

Query: 263 AKDGSGNFTTVGEAVAAAPNSSATRFVIYIKAGAY-FENVEIERKKTNLMFVGDGIGKTL 322
           +KDGSG F T+ EA+  AP  S+ RFVIY+KAG Y  EN+++ RKKTNLMF+GDG GKT+
Sbjct: 283 SKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTV 342

Query: 323 IKGDRNVVDGWTTFRSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNSDFSAFYQCS 382
           I G +++ D  TTF +AT AA G GFI + +TFENYAGP+KHQAVA R   D +  Y+C+
Sbjct: 343 ITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCN 402

Query: 383 FIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 442
            IGYQD LYVHS RQF+R+C++YGT+DFIFGNAAV+ Q+CN+YARKP + Q+   TAQ R
Sbjct: 403 IIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNR 462

Query: 443 DDPNQNTGISILNCKVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 502
            DPNQNTGISI  CK+ A  DL     ++ TYLGRPWKLYSR V++ S +G  +DP GWL
Sbjct: 463 KDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWL 522

Query: 503 EWNGTFALDTLYYGEYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAFIQGSSWLDS 562
           EWNG FALD+LYYGEY+N+G GS    RV WPGY VIT++ EAS+FTV  FI GSSWL S
Sbjct: 523 EWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPS 582

BLAST of Sed0010801 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.5e-135
Identity = 260/535 (48.60%), Postives = 333/535 (62.24%), Query Frame = 0

Query: 56  SLCNGTLFPDLCFSKLSSFPQLSSLSPEKLAGAALNFTSDEFHRAYANCTKLKSLLYGSL 115
           S C+ T +P+LC S + +   +   S + +  A++N T       Y    KL     G L
Sbjct: 61  SSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKG-L 120

Query: 116 NPTDRHALDDCLELLDGSIAELQASISDL---APSECAASHSHDLLTLVSAAMTNHRTCI 175
            P ++ AL DCLE +D ++ EL  ++ DL      +    H+ DL TL+S+A+TN  TC+
Sbjct: 121 TPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCL 180

Query: 176 DGFYNSSGDVRSRVGMYFGEKLVRHL-SIDLAMLKRIPGVDRA----------------- 235
           DGF +   D + R  +  G+  V H+ S  LAM+K +   D A                 
Sbjct: 181 DGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKE 240

Query: 236 -------AAEVFPEYGAVSGGFPTWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGE 295
                  A ++       S G+PTW+S  DRRLLQ   +  K D  VA DGSG F TV  
Sbjct: 241 ENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAA 300

Query: 296 AVAAAPNSSATRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGDRNVVDGWTTF 355
           AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N+MF+GDG  +T+I G RNVVDG TTF
Sbjct: 301 AVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTF 360

Query: 356 RSATVAAVGPGFIAKGITFENYAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLR 415
            SATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFY C  + YQDTLYVHS R
Sbjct: 361 HSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNR 420

Query: 416 QFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRDDPNQNTGISILNC 475
           QF+  C + GT+DFIFGNAAVV Q+C+++AR+PNS Q+N+ TAQGR DPNQNTGI I  C
Sbjct: 421 QFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKC 480

Query: 476 KVEAASDLIPVLSTFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYG 535
           ++ A SDL  V  +F TYLGRPWK YS+TV ++S I  ++ P GW EW GTFAL+TL Y 
Sbjct: 481 RIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYR 540

Query: 536 EYLNRGPGSNTTARVTWPGYRVITNSTEASQFTVDAFIQGSSWLDSTGIPFFSGL 563
           EY N G G+ T  RV W G++VIT + EA ++T   FI G  WL STG PF  GL
Sbjct: 541 EYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896904.11.2e-28187.46pectinesterase-like [Benincasa hispida][more]
XP_022991224.14.6e-27886.94pectinesterase-like [Cucurbita maxima][more]
XP_008451545.26.0e-27886.92PREDICTED: pectinesterase-like [Cucumis melo][more]
KAA0046658.17.9e-27886.74pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [... [more]
XP_023548978.14.3e-27686.40pectinesterase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O813018.5e-15054.25Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
P850761.3e-14578.68Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q425342.6e-14349.13Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
O814153.4e-14349.55Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O048867.4e-13850.58Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JU832.2e-27886.94Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A1S3BSU32.9e-27886.92Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W63.8e-27886.74Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A6J1GNU43.0e-27585.87Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
A0A0A0K5K82.7e-26087.55Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02320.16.0e-15154.25Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.11.9e-14449.13pectin methylesterase 2 [more]
AT4G02300.12.4e-14449.55Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.11.9e-13647.18pectin methylesterase PCR fragment F [more]
AT3G14310.13.5e-13548.60pectin methylesterase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 49..204
e-value: 1.4E-25
score: 101.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 27..204
e-value: 3.4E-22
score: 76.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 56..204
e-value: 3.5E-24
score: 85.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 56..210
e-value: 4.2E-27
score: 96.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 56..208
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 251..547
e-value: 1.7E-146
score: 486.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 245..562
e-value: 1.9E-123
score: 413.9
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 16..559
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 16..559
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 56..210
e-value: 1.67418E-35
score: 128.71
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 395..404
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 249..561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0010801.1Sed0010801.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity