Sed0010326 (gene) Chayote v1

Overview
NameSed0010326
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCaM_binding domain-containing protein
LocationLG11: 34419326 .. 34421434 (+)
RNA-Seq ExpressionSed0010326
SyntenySed0010326
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAGAAGAGTGATGTGCCGGTGCCCCTAGAAGTGAATGTGACTGCGGCTGCAGGAACTAGCTCGAGAAGAAGCTCTATGGGATGGACAATTTTTTCGAATAGCAGAGAAAAAGTGGTGCCTCGTTATCTAAGAGCATCTACTGGTTCCTGTCATGATATTTGCAAATATGGGAGAAACCATACTTTTGAAACCAAGTCGAGACAACCAATACCGAAAAATGTGACAAGAGGACCACTTGAGGGCGTAAGAACGGTTGATAGTGTTGTACTTCCAGAGAGAAAGAAAACAGGTGGAACTTTGACAGATAGTGTGGTAATTCCTGAGAGAAAGATAGCAGCTACGACCCAAATGTCAAAAAATATGACAAGAACGTCACTCGATGGTCGAAATTCGGTTGATAGTATGGTACTTTCAGAGGGAAAGAAAAAAAGTTCTACTCGAATGCTAAAAAATGAAGCAAGGCAATCACTCGATGATAGAAGTTTAGTTGATAGTATGGTACTTTCGGAGAGAAAGAAAACAAGTTGGACCCGAATACTAAAAAACGTGGCAAGAAAATCACTCGATGGTGGAAGTTTGATAGATAGTACGGTACGTCATGAGAGACAGAAAACAAGTTCAACTCGAATGCGCAAAAATGTGGCTAGAAAATCTTTTAATGGTGAAAGTTCGGTAGATAGTATGATACTTCCTGAGAGAAGGAAAACAACTTCGACTCGAGCCAAGGCTGAATCTCTATCAACTTCCAGCATACCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCACTAGATTCCAGCTTCCCAAGACTATTGCAGAGGGAAGTATTGAATGAGAAGAAGAAAAAATTGTTGCCAAGTCCAATGCAGAGAGAAGTTTCGAAGGAAAGGAAGAAAAAATTGTTGGGTGATCCGAGAACTTCACCTAAGTCAAGATCACATACAGAAAATGCACTGAAAAAATTGAAGCCTGAAACTTCTTCAGCTATTAGATATCCAGAAGACTCTGTAGTTCACAGTGTTGCAAAAGCCAAGGAGAGAAAGCTACCTGAAAAATCTGATAAAATTTCAAAGCTAAAGTCGATTAAAGTAAATCCATTGAGATCTGTTGTGTCTCTCATCAATTCGAGAAGAAATAAGGATTCCAAAATGGGAAAAAGTTCTGGAACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAGTCTCAACAGGTTCATTATCTTCGAACTCTATCGATGGAACTACAAACTTACCATGTATTAACTTGAAAGAAGTCCCGCTTGAAAATCATAACAAGATCGAAATATCTAAATGCGAACAAGTCCGGAGTGAAGAGGTCGAAGGAGAAATCTTTCGAAATGAAGAGGTCCAAGGAGAAACCTTCCATAGTGAAAAGGTTCAAGAGAAAACCTTATATGTGATTGAGATAGAAAATGAGGAGATACCTCATCAACCTGACCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTGAATCGTTATCACTGACAGCATCTCCGCATATCTCGGCTGATGTAAAAGATCAAGATGTATCTGAATACACAGAAAGTGAAGAAAAGGAGGACTCTTACTCTGAGAGCAATGAAATGGGTAGCATGAAAGCAAATGATGTTTCAAGTGAAGGTGGTAAAACAGGAGGATCCCAAAATCTTAGGTTGTTGAAAGCTAATGAAATGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAGTTATCGACATTCGTTCCGAGAATTATAGTCCGAGGAGGCTTAAATTTAGGCCTGTAAGGCTGTTGGGAGAGAGTCAAAGCGCTGGGGATGGCCTTAGGAAAAACTTCAAGAGGGGAAAAGAAGTTGACAGTAACACAACAACAGCCAAAGAAACCGTTGTTCTGAGGCATCATGATGTGCAGGAGAAAAAAGATGCACATGGATTGTATAACAATGTCATTGAAGAAACTGCGAGTAAACTTGTCGAAACTCGAAATAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTAATCTTCCTGCAAGATAGAAAACCTTCTCTTAGGCTTGACACTGTAAGTTGA

mRNA sequence

ATGGCTGAGAAGAGTGATGTGCCGGTGCCCCTAGAAGTGAATGTGACTGCGGCTGCAGGAACTAGCTCGAGAAGAAGCTCTATGGGATGGACAATTTTTTCGAATAGCAGAGAAAAAGTGGTGCCTCGTTATCTAAGAGCATCTACTGGTTCCTGTCATGATATTTGCAAATATGGGAGAAACCATACTTTTGAAACCAAGTCGAGACAACCAATACCGAAAAATGTGACAAGAGGACCACTTGAGGGCGTAAGAACGGTTGATAGTGTTGTACTTCCAGAGAGAAAGAAAACAGGTGGAACTTTGACAGATAGTGTGGTAATTCCTGAGAGAAAGATAGCAGCTACGACCCAAATGTCAAAAAATATGACAAGAACGTCACTCGATGGTCGAAATTCGGTTGATAGTATGGTACTTTCAGAGGGAAAGAAAAAAAGTTCTACTCGAATGCTAAAAAATGAAGCAAGGCAATCACTCGATGATAGAAGTTTAGTTGATAGTATGGTACTTTCGGAGAGAAAGAAAACAAGTTGGACCCGAATACTAAAAAACGTGGCAAGAAAATCACTCGATGGTGGAAGTTTGATAGATAGTACGGTACGTCATGAGAGACAGAAAACAAGTTCAACTCGAATGCGCAAAAATGTGGCTAGAAAATCTTTTAATGGTGAAAGTTCGGTAGATAGTATGATACTTCCTGAGAGAAGGAAAACAACTTCGACTCGAGCCAAGGCTGAATCTCTATCAACTTCCAGCATACCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCACTAGATTCCAGCTTCCCAAGACTATTGCAGAGGGAAGTATTGAATGAGAAGAAGAAAAAATTGTTGCCAAGTCCAATGCAGAGAGAAGTTTCGAAGGAAAGGAAGAAAAAATTGTTGGGTGATCCGAGAACTTCACCTAAGTCAAGATCACATACAGAAAATGCACTGAAAAAATTGAAGCCTGAAACTTCTTCAGCTATTAGATATCCAGAAGACTCTGTAGTTCACAGTGTTGCAAAAGCCAAGGAGAGAAAGCTACCTGAAAAATCTGATAAAATTTCAAAGCTAAAGTCGATTAAAGTAAATCCATTGAGATCTGTTGTGTCTCTCATCAATTCGAGAAGAAATAAGGATTCCAAAATGGGAAAAAGTTCTGGAACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAGTCTCAACAGGTTCATTATCTTCGAACTCTATCGATGGAACTACAAACTTACCATGTATTAACTTGAAAGAAGTCCCGCTTGAAAATCATAACAAGATCGAAATATCTAAATGCGAACAAGTCCGGAGTGAAGAGGTCGAAGGAGAAATCTTTCGAAATGAAGAGGTCCAAGGAGAAACCTTCCATAGTGAAAAGGTTCAAGAGAAAACCTTATATGTGATTGAGATAGAAAATGAGGAGATACCTCATCAACCTGACCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTGAATCGTTATCACTGACAGCATCTCCGCATATCTCGGCTGATGTAAAAGATCAAGATGTATCTGAATACACAGAAAGTGAAGAAAAGGAGGACTCTTACTCTGAGAGCAATGAAATGGGTAGCATGAAAGCAAATGATGTTTCAAGTGAAGGTGGTAAAACAGGAGGATCCCAAAATCTTAGGTTGTTGAAAGCTAATGAAATGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAGTTATCGACATTCGTTCCGAGAATTATAGTCCGAGGAGGCTTAAATTTAGGCCTGTAAGGCTGTTGGGAGAGAGTCAAAGCGCTGGGGATGGCCTTAGGAAAAACTTCAAGAGGGGAAAAGAAGTTGACAGTAACACAACAACAGCCAAAGAAACCGTTGTTCTGAGGCATCATGATGTGCAGGAGAAAAAAGATGCACATGGATTGTATAACAATGTCATTGAAGAAACTGCGAGTAAACTTGTCGAAACTCGAAATAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTAATCTTCCTGCAAGATAGAAAACCTTCTCTTAGGCTTGACACTGTAAGTTGA

Coding sequence (CDS)

ATGGCTGAGAAGAGTGATGTGCCGGTGCCCCTAGAAGTGAATGTGACTGCGGCTGCAGGAACTAGCTCGAGAAGAAGCTCTATGGGATGGACAATTTTTTCGAATAGCAGAGAAAAAGTGGTGCCTCGTTATCTAAGAGCATCTACTGGTTCCTGTCATGATATTTGCAAATATGGGAGAAACCATACTTTTGAAACCAAGTCGAGACAACCAATACCGAAAAATGTGACAAGAGGACCACTTGAGGGCGTAAGAACGGTTGATAGTGTTGTACTTCCAGAGAGAAAGAAAACAGGTGGAACTTTGACAGATAGTGTGGTAATTCCTGAGAGAAAGATAGCAGCTACGACCCAAATGTCAAAAAATATGACAAGAACGTCACTCGATGGTCGAAATTCGGTTGATAGTATGGTACTTTCAGAGGGAAAGAAAAAAAGTTCTACTCGAATGCTAAAAAATGAAGCAAGGCAATCACTCGATGATAGAAGTTTAGTTGATAGTATGGTACTTTCGGAGAGAAAGAAAACAAGTTGGACCCGAATACTAAAAAACGTGGCAAGAAAATCACTCGATGGTGGAAGTTTGATAGATAGTACGGTACGTCATGAGAGACAGAAAACAAGTTCAACTCGAATGCGCAAAAATGTGGCTAGAAAATCTTTTAATGGTGAAAGTTCGGTAGATAGTATGATACTTCCTGAGAGAAGGAAAACAACTTCGACTCGAGCCAAGGCTGAATCTCTATCAACTTCCAGCATACCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCACTAGATTCCAGCTTCCCAAGACTATTGCAGAGGGAAGTATTGAATGAGAAGAAGAAAAAATTGTTGCCAAGTCCAATGCAGAGAGAAGTTTCGAAGGAAAGGAAGAAAAAATTGTTGGGTGATCCGAGAACTTCACCTAAGTCAAGATCACATACAGAAAATGCACTGAAAAAATTGAAGCCTGAAACTTCTTCAGCTATTAGATATCCAGAAGACTCTGTAGTTCACAGTGTTGCAAAAGCCAAGGAGAGAAAGCTACCTGAAAAATCTGATAAAATTTCAAAGCTAAAGTCGATTAAAGTAAATCCATTGAGATCTGTTGTGTCTCTCATCAATTCGAGAAGAAATAAGGATTCCAAAATGGGAAAAAGTTCTGGAACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAGTCTCAACAGGTTCATTATCTTCGAACTCTATCGATGGAACTACAAACTTACCATGTATTAACTTGAAAGAAGTCCCGCTTGAAAATCATAACAAGATCGAAATATCTAAATGCGAACAAGTCCGGAGTGAAGAGGTCGAAGGAGAAATCTTTCGAAATGAAGAGGTCCAAGGAGAAACCTTCCATAGTGAAAAGGTTCAAGAGAAAACCTTATATGTGATTGAGATAGAAAATGAGGAGATACCTCATCAACCTGACCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTGAATCGTTATCACTGACAGCATCTCCGCATATCTCGGCTGATGTAAAAGATCAAGATGTATCTGAATACACAGAAAGTGAAGAAAAGGAGGACTCTTACTCTGAGAGCAATGAAATGGGTAGCATGAAAGCAAATGATGTTTCAAGTGAAGGTGGTAAAACAGGAGGATCCCAAAATCTTAGGTTGTTGAAAGCTAATGAAATGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAGTTATCGACATTCGTTCCGAGAATTATAGTCCGAGGAGGCTTAAATTTAGGCCTGTAAGGCTGTTGGGAGAGAGTCAAAGCGCTGGGGATGGCCTTAGGAAAAACTTCAAGAGGGGAAAAGAAGTTGACAGTAACACAACAACAGCCAAAGAAACCGTTGTTCTGAGGCATCATGATGTGCAGGAGAAAAAAGATGCACATGGATTGTATAACAATGTCATTGAAGAAACTGCGAGTAAACTTGTCGAAACTCGAAATAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTAATCTTCCTGCAAGATAGAAAACCTTCTCTTAGGCTTGACACTGTAAGTTGA

Protein sequence

MAEKSDVPVPLEVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQPIPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGRNSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLDGGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTSSIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPCINLKEVPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIEIENEEIPHQPDQNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGSMKANDVSSEGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQEKKDAHGLYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPSLRLDTVS
Homology
BLAST of Sed0010326 vs. NCBI nr
Match: XP_022990927.1 (uncharacterized protein LOC111487671 isoform X2 [Cucurbita maxima])

HSP 1 Score: 707.6 bits (1825), Expect = 1.1e-199
Identity = 441/690 (63.91%), Postives = 507/690 (73.48%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +P           +QREV N +KKKLL SP  REV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTKPS---------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIEIENEEIPHQ 491
              + HNK ++S  E+V+SEEV GE F++EEVQGETF S +VQEKTLYVI++ENEE+PHQ
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMPHQ 486

Query: 492 PD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGS 551
            D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + DS+SESNE GS
Sbjct: 487 SDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETENDSFSESNEYGS 546

Query: 552 MKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPV 611
           M+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ SPRRLKFR  
Sbjct: 547 METDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRLKFRRG 606

Query: 612 RLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHGLYNNVIEETA 671
           RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA GL+NNVIEETA
Sbjct: 607 RLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNVIEETA 666

Query: 672 SKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           SKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 SKLVETRKSKVKALVGAFETVISLQDGRLS 677

BLAST of Sed0010326 vs. NCBI nr
Match: XP_023541286.1 (uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023541294.1 uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707.6 bits (1825), Expect = 1.1e-199
Identity = 444/714 (62.18%), Postives = 512/714 (71.71%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHD CKYG+NHTFETKSR+P
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRRP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+ DSVVL ERKKTGG   D VVIPERK    T+ SK+MTR SLD  
Sbjct: 67  MA--MTRKSLDGGRSEDSVVLRERKKTGGGSVDGVVIPERKKTIMTERSKHMTRKSLDDG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +S RM KN AR+SL      D +VL ERKKT+ TR+ KNVAR SLD
Sbjct: 127 SSVDSMVLPERKKTTSPRMSKNVARKSL------DGVVLLERKKTTSTRMSKNVARISLD 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNG-------------------ESSVDS--- 251
           GGS +D  V  ER+KT+S RM KNVAR S +G                     S+DS   
Sbjct: 187 GGSSVDGVVLLERKKTASPRMSKNVARSSIDGVVLLERKKTISTRMLKNVARISLDSGSS 246

Query: 252 --MILPERRKTTSTRAKAESLSTSSIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKK 311
              ILPER+KT STR KAE  STS +  A++TT  RP           +QREV N +KKK
Sbjct: 247 IRTILPERKKTASTRMKAELSSTSRMSGANVTTLTRPS---------PVQREVSNGRKKK 306

Query: 312 LLPSPMQREVSKERKKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAK 371
           LL SP QREV  ERKKKLL +P+   KSRSHT N L   KPETS+A R PEDS V  +AK
Sbjct: 307 LLSSPTQREVLNERKKKLLAEPKALAKSRSHTANTLNNSKPETSTATRNPEDSAV--LAK 366

Query: 372 AKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVST 431
            KERKLPEKS+   KL+SIKVNPLRS VS   SRR   SKM K+ GTSKVAAKKVVA ST
Sbjct: 367 TKERKLPEKSENFVKLRSIKVNPLRSAVSTDGSRRTNGSKMVKNLGTSKVAAKKVVATST 426

Query: 432 GSLSSNSIDGTTNLPC---INLKEVPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGET 491
           GS SSNSI G  NL     +NLK  P + HNK ++S  +QV+ EEV GE F++EEVQGET
Sbjct: 427 GSFSSNSIYGNANLTARKRVNLKGSPHKTHNKNKLSDHQQVQGEEVRGETFQSEEVQGET 486

Query: 492 FHSEKVQEKTLYVIEIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKD 551
           F S +VQEKTLYVI++ENEE+PH+PD QNET DN+EPLPSSPP SLS  ++ +I A VKD
Sbjct: 487 FRSVEVQEKTLYVIKVENEEMPHEPDGQNETFDNVEPLPSSPPNSLSPPSAQYILAKVKD 546

Query: 552 QDVSEYTESEEKEDSYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLS 611
           QDVSEYTESE + DS+SESNE GSM+ ++VSS EGG+    QN R+L   E DPQSTKLS
Sbjct: 547 QDVSEYTESETENDSFSESNEYGSMETDNVSSEEGGQNARYQNPRMLHNKEEDPQSTKLS 606

Query: 612 FRRGKVIDIRSENYSPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVL 671
           FRRGK+IDI S++ SPRRLKFR  RLLGESQ   +GLRKNFK G EVD +T TA+E VVL
Sbjct: 607 FRRGKIIDIPSKSNSPRRLKFRRGRLLGESQRDAEGLRKNFKMGTEVDGDTATAQEIVVL 666

Query: 672 RHHDVQE-KKDAHGLYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           RH DVQ+ +KDA GL+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 RHQDVQQRRKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 701

BLAST of Sed0010326 vs. NCBI nr
Match: XP_023541278.1 (uncharacterized protein LOC111801497 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707.6 bits (1825), Expect = 1.1e-199
Identity = 444/714 (62.18%), Postives = 512/714 (71.71%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHD CKYG+NHTFETKSR+P
Sbjct: 29  DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRRP 88

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+ DSVVL ERKKTGG   D VVIPERK    T+ SK+MTR SLD  
Sbjct: 89  MA--MTRKSLDGGRSEDSVVLRERKKTGGGSVDGVVIPERKKTIMTERSKHMTRKSLDDG 148

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +S RM KN AR+SL      D +VL ERKKT+ TR+ KNVAR SLD
Sbjct: 149 SSVDSMVLPERKKTTSPRMSKNVARKSL------DGVVLLERKKTTSTRMSKNVARISLD 208

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNG-------------------ESSVDS--- 251
           GGS +D  V  ER+KT+S RM KNVAR S +G                     S+DS   
Sbjct: 209 GGSSVDGVVLLERKKTASPRMSKNVARSSIDGVVLLERKKTISTRMLKNVARISLDSGSS 268

Query: 252 --MILPERRKTTSTRAKAESLSTSSIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKK 311
              ILPER+KT STR KAE  STS +  A++TT  RP           +QREV N +KKK
Sbjct: 269 IRTILPERKKTASTRMKAELSSTSRMSGANVTTLTRPS---------PVQREVSNGRKKK 328

Query: 312 LLPSPMQREVSKERKKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAK 371
           LL SP QREV  ERKKKLL +P+   KSRSHT N L   KPETS+A R PEDS V  +AK
Sbjct: 329 LLSSPTQREVLNERKKKLLAEPKALAKSRSHTANTLNNSKPETSTATRNPEDSAV--LAK 388

Query: 372 AKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVST 431
            KERKLPEKS+   KL+SIKVNPLRS VS   SRR   SKM K+ GTSKVAAKKVVA ST
Sbjct: 389 TKERKLPEKSENFVKLRSIKVNPLRSAVSTDGSRRTNGSKMVKNLGTSKVAAKKVVATST 448

Query: 432 GSLSSNSIDGTTNLPC---INLKEVPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGET 491
           GS SSNSI G  NL     +NLK  P + HNK ++S  +QV+ EEV GE F++EEVQGET
Sbjct: 449 GSFSSNSIYGNANLTARKRVNLKGSPHKTHNKNKLSDHQQVQGEEVRGETFQSEEVQGET 508

Query: 492 FHSEKVQEKTLYVIEIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKD 551
           F S +VQEKTLYVI++ENEE+PH+PD QNET DN+EPLPSSPP SLS  ++ +I A VKD
Sbjct: 509 FRSVEVQEKTLYVIKVENEEMPHEPDGQNETFDNVEPLPSSPPNSLSPPSAQYILAKVKD 568

Query: 552 QDVSEYTESEEKEDSYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLS 611
           QDVSEYTESE + DS+SESNE GSM+ ++VSS EGG+    QN R+L   E DPQSTKLS
Sbjct: 569 QDVSEYTESETENDSFSESNEYGSMETDNVSSEEGGQNARYQNPRMLHNKEEDPQSTKLS 628

Query: 612 FRRGKVIDIRSENYSPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVL 671
           FRRGK+IDI S++ SPRRLKFR  RLLGESQ   +GLRKNFK G EVD +T TA+E VVL
Sbjct: 629 FRRGKIIDIPSKSNSPRRLKFRRGRLLGESQRDAEGLRKNFKMGTEVDGDTATAQEIVVL 688

Query: 672 RHHDVQE-KKDAHGLYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           RH DVQ+ +KDA GL+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 689 RHQDVQQRRKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 723

BLAST of Sed0010326 vs. NCBI nr
Match: XP_022990925.1 (uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima] >XP_022990926.1 uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima])

HSP 1 Score: 701.0 bits (1808), Expect = 1.0e-197
Identity = 442/700 (63.14%), Postives = 507/700 (72.43%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +P           +QREV N +KKKLL SP  REV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTKPS---------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQV----------RSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVI 491
              + HNK ++S  E+V          RSEEV GE F++EEVQGETF S +VQEKTLYVI
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVI 486

Query: 492 EIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKED 551
           ++ENEE+PHQ D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + D
Sbjct: 487 KVENEEMPHQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETEND 546

Query: 552 SYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENY 611
           S+SESNE GSM+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ 
Sbjct: 547 SFSESNEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSN 606

Query: 612 SPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHG 671
           SPRRLKFR  RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA G
Sbjct: 607 SPRRLKFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQG 666

Query: 672 LYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           L+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 LFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 687

BLAST of Sed0010326 vs. NCBI nr
Match: XP_022990929.1 (uncharacterized protein LOC111487671 isoform X3 [Cucurbita maxima])

HSP 1 Score: 684.1 bits (1764), Expect = 1.3e-192
Identity = 433/700 (61.86%), Postives = 497/700 (71.00%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +                          PSP+QREV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTK--------------------------PSPVQREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQV----------RSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVI 491
              + HNK ++S  E+V          RSEEV GE F++EEVQGETF S +VQEKTLYVI
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVI 486

Query: 492 EIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKED 551
           ++ENEE+PHQ D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + D
Sbjct: 487 KVENEEMPHQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETEND 546

Query: 552 SYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENY 611
           S+SESNE GSM+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ 
Sbjct: 547 SFSESNEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSN 606

Query: 612 SPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHG 671
           SPRRLKFR  RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA G
Sbjct: 607 SPRRLKFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQG 666

Query: 672 LYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           L+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 LFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 670

BLAST of Sed0010326 vs. ExPASy TrEMBL
Match: A0A6J1JK93 (uncharacterized protein LOC111487671 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 5.2e-200
Identity = 441/690 (63.91%), Postives = 507/690 (73.48%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +P           +QREV N +KKKLL SP  REV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTKPS---------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIEIENEEIPHQ 491
              + HNK ++S  E+V+SEEV GE F++EEVQGETF S +VQEKTLYVI++ENEE+PHQ
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMPHQ 486

Query: 492 PD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGS 551
            D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + DS+SESNE GS
Sbjct: 487 SDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETENDSFSESNEYGS 546

Query: 552 MKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPV 611
           M+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ SPRRLKFR  
Sbjct: 547 METDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRLKFRRG 606

Query: 612 RLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHGLYNNVIEETA 671
           RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA GL+NNVIEETA
Sbjct: 607 RLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNVIEETA 666

Query: 672 SKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           SKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 SKLVETRKSKVKALVGAFETVISLQDGRLS 677

BLAST of Sed0010326 vs. ExPASy TrEMBL
Match: A0A6J1JPA7 (uncharacterized protein LOC111487671 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 4.9e-198
Identity = 442/700 (63.14%), Postives = 507/700 (72.43%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +P           +QREV N +KKKLL SP  REV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTKPS---------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQV----------RSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVI 491
              + HNK ++S  E+V          RSEEV GE F++EEVQGETF S +VQEKTLYVI
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVI 486

Query: 492 EIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKED 551
           ++ENEE+PHQ D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + D
Sbjct: 487 KVENEEMPHQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETEND 546

Query: 552 SYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENY 611
           S+SESNE GSM+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ 
Sbjct: 547 SFSESNEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSN 606

Query: 612 SPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHG 671
           SPRRLKFR  RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA G
Sbjct: 607 SPRRLKFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQG 666

Query: 672 LYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           L+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 LFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 687

BLAST of Sed0010326 vs. ExPASy TrEMBL
Match: A0A6J1JTD5 (uncharacterized protein LOC111487671 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 6.1e-193
Identity = 433/700 (61.86%), Postives = 497/700 (71.00%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGTSSRRSS GW   SNS EK VP Y R STGSCHDICKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VD VVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  VA--MTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSG 126

Query: 132 NSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVARKSLD 191
           +SVDSMVL E KK +STRM KN AR SLD  S VD +VL ERKKT+  R+ KNVAR S+D
Sbjct: 127 SSVDSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSID 186

Query: 192 GGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTS 251
           G  L+      ER+KT STRM KNV R S +  SSV+  ILPER+KT STR KAE  STS
Sbjct: 187 GVVLL------ERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTS 246

Query: 252 SIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRT 311
            +  A++TT  +                          PSP+QREV  ERK KLL +P+ 
Sbjct: 247 RMSGANVTTLTK--------------------------PSPVQREVLNERKNKLLAEPKA 306

Query: 312 SPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPL 371
             KSRSHT N LK  KPETS+A R PEDS V  +AK KERKLPEKS+   KLKSIKVNPL
Sbjct: 307 LAKSRSHTANMLKNSKPETSTATRNPEDSAV--LAKTKERKLPEKSENFVKLKSIKVNPL 366

Query: 372 RSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKE 431
           RS VS   SRR   SKM K+ GTSKVAAKKVVA STGS SSNSI G  NL     ++LK 
Sbjct: 367 RSAVSTDGSRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKG 426

Query: 432 VPLENHNKIEISKCEQV----------RSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVI 491
              + HNK ++S  E+V          RSEEV GE F++EEVQGETF S +VQEKTLYVI
Sbjct: 427 GLHKTHNKNKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVI 486

Query: 492 EIENEEIPHQPD-QNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKED 551
           ++ENEE+PHQ D QNET DNMEPLPSS   SLS  ++ +I A+VKDQDVSEYTESE + D
Sbjct: 487 KVENEEMPHQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEYTESETEND 546

Query: 552 SYSESNEMGSMKANDVSS-EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENY 611
           S+SESNE GSM+ ++ SS EGG+    QN R+L   E DPQSTKLSFRRGK+IDI +++ 
Sbjct: 547 SFSESNEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSN 606

Query: 612 SPRRLKFRPVRLLGESQSAGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQE-KKDAHG 671
           SPRRLKFR  RLLGESQ   DGLRKNFK G EVD +T TA+E VVLRH DVQ+ +KDA G
Sbjct: 607 SPRRLKFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQG 666

Query: 672 LYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           L+NNVIEETASKLVETR SKVKALVGAFETVI LQD + S
Sbjct: 667 LFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGRLS 670

BLAST of Sed0010326 vs. ExPASy TrEMBL
Match: A0A6J1GZM4 (uncharacterized protein LOC111459082 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459082 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 5.7e-191
Identity = 435/741 (58.70%), Postives = 504/741 (68.02%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGT SRRSS GW   SNS EK VP Y R STGSCHD CKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTGSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VDSVVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  M--TMTRKSLDGGRSVDSVVLRERKKTGGGSGDSVVIPERKKTIMTERSKHMTRKSLDDG 126

Query: 132 NSVDSMVLSEGKKKSSTRML----------------------KNEARQSLDDRSLVDSMV 191
           +SVDSMVL E KK +S RML                      KN AR+SLD  S VD +V
Sbjct: 127 SSVDSMVLPERKKTTSPRMLKNVARISLDGESSVKTTSPRMSKNVARKSLDGGSSVDGVV 186

Query: 192 LSERKKTSWTRILKNVARKSLDGGSLIDSTVRHERQKTS--------------------- 251
           L ERKKT+ TR+ KNVAR SLDGGS +D  V  ER+KT+                     
Sbjct: 187 LLERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVGRSSIDGVVLLER 246

Query: 252 ----STRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTSSIPEADLTTFIRP 311
               STRM KNVAR S +  SSV+  ILPER+KT STR KAE  STS +  A++TT  R 
Sbjct: 247 KKTISTRMSKNVARISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTTLTR- 306

Query: 312 KLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRTSPKSRSHTENALK 371
                                    PSP+QREV  ERKKKLL +P+   KSRSHT N LK
Sbjct: 307 -------------------------PSPVQREVLNERKKKLLAEPKALAKSRSHTANTLK 366

Query: 372 KLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRRNK 431
             KPETS+A R PEDS V  +AK KERKLPEK++   KLKSIKVNPLRS VS   SRR  
Sbjct: 367 NSKPETSTATRNPEDSAV--LAKTKERKLPEKTENFVKLKSIKVNPLRSAVSTDGSRRTN 426

Query: 432 DSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKEVPLENHNKIEISK 491
            SKM K+ GTSKVAAK+VVA S+GS SSNSI G  NL     ++LK    + HNK ++S 
Sbjct: 427 GSKMVKNLGTSKVAAKRVVATSSGSFSSNSIYGNANLSARKRVSLKGGLHKTHNKNKLSD 486

Query: 492 CEQVRSEEVEGEIFRNEEV-----QGETFHSEKVQEKTLYVIEIENEEIPHQPD-QNETD 551
            +QV+SEEV+ + FR+EEV     QGETF S +VQEKTLYVI++ENEE+PHQ D QNET 
Sbjct: 487 HQQVQSEEVDKKTFRSEEVRGETFQGETFRSVEVQEKTLYVIKVENEEMPHQTDGQNETF 546

Query: 552 DNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGSMKANDVSS 611
           DN+EPLPSSPP SLS  ++ +I A VKDQDVSEYTESE + DS+SESNE GSM+ ++ SS
Sbjct: 547 DNVEPLPSSPPNSLSPPSAQYILAKVKDQDVSEYTESETENDSFSESNEYGSMETDNASS 606

Query: 612 -EGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPVRLLGESQS 671
            EGG+    QN R+L   E DPQSTKLSFRRGK+IDI S++ SPRRLKFR  RLLGES+ 
Sbjct: 607 EEGGQNARYQNPRMLHNKEEDPQSTKLSFRRGKIIDIPSKSNSPRRLKFRRGRLLGESRR 666

Query: 672 AGDGLRKNFKRGKEVDSNTTTAKETVVLRHHDVQEKKDAHGLYNNVIEETASKLVETRNS 696
             DGLRKNFK G EVD +T  A+E VVLRH DVQ++KDA GL+NNVIEETASKLVETR S
Sbjct: 667 DADGLRKNFKMGTEVDGDTAMAQEIVVLRHQDVQQRKDAQGLFNNVIEETASKLVETRKS 717

BLAST of Sed0010326 vs. ExPASy TrEMBL
Match: A0A6J1H132 (uncharacterized protein LOC111459082 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459082 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 3.7e-190
Identity = 433/736 (58.83%), Postives = 499/736 (67.80%), Query Frame = 0

Query: 12  EVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQP 71
           +V +T  AGT SRRSS GW   SNS EK VP Y R STGSCHD CKYG+NHTFETKSRQP
Sbjct: 7   DVQMTTTAGTGSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRQP 66

Query: 72  IPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAATTQMSKNMTRTSLDGR 131
           +   +TR  L+G R+VDSVVL ERKKTGG   DSVVIPERK    T+ SK+MTR SLD  
Sbjct: 67  M--TMTRKSLDGGRSVDSVVLRERKKTGGGSGDSVVIPERKKTIMTERSKHMTRKSLDDG 126

Query: 132 NSVDSMVLSEGKKKSSTRML----------------------KNEARQSLDDRSLVDSMV 191
           +SVDSMVL E KK +S RML                      KN AR+SLD  S VD +V
Sbjct: 127 SSVDSMVLPERKKTTSPRMLKNVARISLDGESSVKTTSPRMSKNVARKSLDGGSSVDGVV 186

Query: 192 LSERKKTSWTRILKNVARKSLDGGSLIDSTVRHERQKTS--------------------- 251
           L ERKKT+ TR+ KNVAR SLDGGS +D  V  ER+KT+                     
Sbjct: 187 LLERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVGRSSIDGVVLLER 246

Query: 252 ----STRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAESLSTSSIPEADLTTFIRP 311
               STRM KNVAR S +  SSV+  ILPER+KT STR KAE  STS +  A++TT  R 
Sbjct: 247 KKTISTRMSKNVARISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTTLTR- 306

Query: 312 KLSLDSSFPRLLQREVLNEKKKKLLPSPMQREVSKERKKKLLGDPRTSPKSRSHTENALK 371
                                    PSP+QREV  ERKKKLL +P+   KSRSHT N LK
Sbjct: 307 -------------------------PSPVQREVLNERKKKLLAEPKALAKSRSHTANTLK 366

Query: 372 KLKPETSSAIRYPEDSVVHSVAKAKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRRNK 431
             KPETS+A R PEDS V  +AK KERKLPEK++   KLKSIKVNPLRS VS   SRR  
Sbjct: 367 NSKPETSTATRNPEDSAV--LAKTKERKLPEKTENFVKLKSIKVNPLRSAVSTDGSRRTN 426

Query: 432 DSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPC---INLKEVPLENHNKIEISK 491
            SKM K+ GTSKVAAK+VVA S+GS SSNSI G  NL     ++LK    + HNK ++S 
Sbjct: 427 GSKMVKNLGTSKVAAKRVVATSSGSFSSNSIYGNANLSARKRVSLKGGLHKTHNKNKLSD 486

Query: 492 CEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIEIENEEIPHQPD-QNETDDNMEP 551
            +QV+SEEV GE F     QGETF S +VQEKTLYVI++ENEE+PHQ D QNET DN+EP
Sbjct: 487 HQQVQSEEVRGETF-----QGETFRSVEVQEKTLYVIKVENEEMPHQTDGQNETFDNVEP 546

Query: 552 LPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGSMKANDVSS-EGGK 611
           LPSSPP SLS  ++ +I A VKDQDVSEYTESE + DS+SESNE GSM+ ++ SS EGG+
Sbjct: 547 LPSSPPNSLSPPSAQYILAKVKDQDVSEYTESETENDSFSESNEYGSMETDNASSEEGGQ 606

Query: 612 TGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPVRLLGESQSAGDGL 671
               QN R+L   E DPQSTKLSFRRGK+IDI S++ SPRRLKFR  RLLGES+   DGL
Sbjct: 607 NARYQNPRMLHNKEEDPQSTKLSFRRGKIIDIPSKSNSPRRLKFRRGRLLGESRRDADGL 666

Query: 672 RKNFKRGKEVDSNTTTAKETVVLRHHDVQEKKDAHGLYNNVIEETASKLVETRNSKVKAL 696
           RKNFK G EVD +T  A+E VVLRH DVQ++KDA GL+NNVIEETASKLVETR SKVKAL
Sbjct: 667 RKNFKMGTEVDGDTAMAQEIVVLRHQDVQQRKDAQGLFNNVIEETASKLVETRKSKVKAL 707

BLAST of Sed0010326 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 119.8 bits (299), Expect = 8.9e-27
Identity = 126/375 (33.60%), Postives = 183/375 (48.80%), Query Frame = 0

Query: 325 KLKPETSSAIRYPE--DSVVHSVAKAKERKLPEKSDKISKLKSIKVNPLRSVVSLINSRR 384
           KLKP      R  E  D++   V K     L     K++  K +    L+  +   N  +
Sbjct: 161 KLKPSPDLGSRSSENVDALKPKVLKKSYSALTTSKPKVNHEKVVASPVLKPKMGKRNDGK 220

Query: 385 NKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTTNLPCINLKEVPLENHNKIEISKC 444
           ++D K+ K + +S+VA+KK       SLS          P ++++     +  K +  K 
Sbjct: 221 DEDGKVKKGTVSSRVASKKAPVTPRASLS----------PRLSVRLAGSSSLRKSQSLKA 280

Query: 445 --EQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIEIE-NEEIPHQPDQNETDDNME 504
                R  +    + R +E   +      V+EKTL+V+E+E    +  + DQN+      
Sbjct: 281 ASSSSRQNQKPRPVNRTDEF-NKQLDDYPVEEKTLHVVEMETTNNVVSENDQNQQGFVEP 340

Query: 505 PLPSSPPESLSLTASPHISADVKDQDVSEYTESEEKEDSYSESNEMGSMKANDVSSEGGK 564
            LP  PP            +  KD    E T SE +E  Y+     GS +A     E G 
Sbjct: 341 FLPPLPP----------TQSTPKD---DECTVSETEEYEYTS----GSNEAESEEEEIGL 400

Query: 565 TGGSQNLRLLK--ANEMDPQSTKLSFRRGKVIDIRSENYSPRRLKFRPVRLLGESQSAGD 624
           + G +  R  +   +  D  + KL FRRG ++D  +     R+LKFR  R LGE ++   
Sbjct: 401 SNGEKKPRAARKEGDSADEAARKLRFRRGTIVDPDTVGEKARKLKFRRGRGLGEDKAQDA 460

Query: 625 GLRKNFKRGKEV-DSNTTTAKETVVLRHHDVQEKKDAHGLYNNVIEETASKLVETRNSKV 684
            +R++FK+ +++ +       E VVLRH DVQE KDA GL+NNVIEETASKLVE R SKV
Sbjct: 461 QVRRSFKKREDIREEEVNEDGEKVVLRHQDVQE-KDAQGLFNNVIEETASKLVEARKSKV 506

Query: 685 KALVGAFETVIFLQD 692
           KALVGAFETVI LQ+
Sbjct: 521 KALVGAFETVISLQE 506


HSP 2 Score: 40.4 bits (93), Expect = 6.9e-03
Identity = 67/289 (23.18%), Postives = 118/289 (40.83%), Query Frame = 0

Query: 20  GTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYGRNHTFETKSRQPIPKNVTRG 79
           G +    S G  + S  +EK +P YLRASTGSCHD+CKYG+      K  +   K + + 
Sbjct: 21  GLNPGGDSTGKAMTSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKPWRSSTKKIFKK 80

Query: 80  PLEGVRTVDSVVLP----------ERKKTGGTLTDSVVIPERKIA--ATTQMSKNMTRTS 139
            L+    ++  + P          E +K  GT  DS  + +R++     + +S  M +  
Sbjct: 81  SLDD--NLNETLKPGSSKMKKKVREVEKNEGT-DDSFEVIKREVVKYQASGVSSGMRKPE 140

Query: 140 LDGRNSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTSWTRILKNVAR 199
           +   +S D   + + KKK++   L ++ + S D         L  R   +   +   V +
Sbjct: 141 VLIISSCDETPVKQIKKKTT---LSSKLKPSPD---------LGSRSSENVDALKPKVLK 200

Query: 200 KSLDGGSLIDSTVRHERQKTSSTRMRKNVARKSFNGESSVDSMILPERRKTTSTRAKAES 259
           KS    +     V HE+   S     K   R     E          ++ T S+R  ++ 
Sbjct: 201 KSYSALTTSKPKVNHEKVVASPVLKPKMGKRNDGKDEDG------KVKKGTVSSRVASKK 260

Query: 260 LSTSSIPEADLTTFIRPKLSLDSSFPRLLQ-REVLNEKKKKLLPSPMQR 296
              +  P A L+  +  +L+  SS  +    +   +  ++   P P+ R
Sbjct: 261 APVT--PRASLSPRLSVRLAGSSSLRKSQSLKAASSSSRQNQKPRPVNR 286

BLAST of Sed0010326 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 84.7 bits (208), Expect = 3.2e-16
Identity = 109/411 (26.52%), Postives = 193/411 (46.96%), Query Frame = 0

Query: 302 KKKLLGDPRTSPKSR-------SHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLP 361
           +KK L  P  SP  R        H ++ ++          +   D     V    E +L 
Sbjct: 86  RKKALTKPLLSPSRRCDSVGGFDHAKSQVRNFSSGVCDVKKSHADGTNEKVVSVSESRLA 145

Query: 362 EKSD-KISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSN 421
           + +  K  K K++ V+  R+   +   RR    K+   + T+++A ++   V    ++  
Sbjct: 146 DSTKRKKKKKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRR-STVKRKKMNGG 205

Query: 422 SIDGTTNLPCINLKEVPLENHNKIEISKCEQVRSE--EVEGEIFRNEEVQGETFHSEKVQ 481
           S         + L+   + +      S+C + + E   +   + +  +  G+    + V+
Sbjct: 206 SKAAEQKKAVMALRRASMSSKG---CSRCLKTKKESNSLSVPLKKTRKHVGDKC-KDLVE 265

Query: 482 EKTLYVIEIENEEIPHQPDQNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTE 541
           EKTLYVI++E  +   + + N+      P+   P    S      I  + +D+   E  +
Sbjct: 266 EKTLYVIKMETVDEIVESELNQRCVMDSPI-DDPKSEKSQDKGECIETEHEDESSQEEED 325

Query: 542 SEEKEDSYSESNEMGSM---KANDVSSEGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKV 601
            EE++++ S S +  +    K+   S+E   TG +  LR+               RRGK+
Sbjct: 326 EEEEDENVSVSEDKNTTREGKSKAFSAESAITGNAMKLRI---------------RRGKI 385

Query: 602 IDIRSENYSPRRLKFRPVRLL--GESQSAGDGLRKNFKRGKEVDSN-TTTAKETVVLRHH 661
           ID  SE  SPR+LKF+  +++   ++ S   G R+   +G  + ++     K  VVL+H 
Sbjct: 386 IDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRLKTKGTNLSNDKEQQRKPRVVLKHQ 445

Query: 662 DVQEKKDAHG-LYNNVIEETASKLVETRNSKVKALVGAFETVIFLQDRKPS 696
           D ++K+++   L+N VI+ETA+KLV+TR SKVKALVGAFE+VI LQ++  S
Sbjct: 446 DTEKKRESRVLLFNKVIKETANKLVQTRKSKVKALVGAFESVISLQEKTSS 475


HSP 2 Score: 46.6 bits (109), Expect = 9.6e-05
Identity = 105/429 (24.48%), Postives = 179/429 (41.72%), Query Frame = 0

Query: 1   MAEKS-DVPVPLEVNVTAAAGTSSRRSSMGWTIFSNSREKVVPRYLRASTGSCHDICKYG 60
           MAE++  +P   +VN+      S RR S G   F  ++EKVVP YLR+ TGSCHD CKYG
Sbjct: 1   MAEETVRLPNSPDVNI------SWRRISTGKLSFLYTQEKVVPNYLRSPTGSCHDACKYG 60

Query: 61  RNHTFETKSRQPIPKNVTRGPLEGVRTVDSVVLPERKKTGGTLTDSVVIPERKIAAT--T 120
           R    E K R P  K V+R    G   +DS   P RKK    LT  ++ P R+  +    
Sbjct: 61  RKDESEDKPRVPHRKRVSRS-FSGAINLDS---PLRKK---ALTKPLLSPSRRCDSVGGF 120

Query: 121 QMSKNMTRTSLDGRNSVDSMVLSEGKKKSSTRMLKNEARQSLDDRSLVDSMVLSERKKTS 180
             +K+  R    G   V          K S     NE   S+ +  L DS    ++KK +
Sbjct: 121 DHAKSQVRNFSSGVCDV----------KKSHADGTNEKVVSVSESRLADSTKRKKKKKKT 180

Query: 181 WTRILKNVARKSLDGGSLIDSTVRHERQKTSSTRMRKN-VARKSFNGESSV---DSMILP 240
              + +  A++ ++    + +       +T+   +R++ V RK  NG S        ++ 
Sbjct: 181 -VYVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRRSTVKRKKMNGGSKAAEQKKAVMA 240

Query: 241 ERRKTTSTRAKAESLSTSSIPEADLTTFIRPKLSLDSSFPRLLQREVLNEKKKKLLPSPM 300
            RR + S++  +  L T     +      + +  +      L++ + L   K + +   +
Sbjct: 241 LRRASMSSKGCSRCLKTKKESNSLSVPLKKTRKHVGDKCKDLVEEKTLYVIKMETVDEIV 300

Query: 301 QREVSKERKKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDSVVH-SVAKAKERK 360
           + E+++        D   S KS+   E    + + E+S      E+   + SV++ K   
Sbjct: 301 ESELNQRCVMDSPIDDPKSEKSQDKGECIETEHEDESSQEEEDEEEEDENVSVSEDKNTT 360

Query: 361 LPEKSDKISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSS 420
              KS   S   +I  N ++     +  RR K    G    + +    K   + +G+ ++
Sbjct: 361 REGKSKAFSAESAITGNAMK-----LRIRRGKIIDFGSEGNSPRKLKFKRGKIISGADTT 400

Query: 421 NSIDGTTNL 422
           +   G   L
Sbjct: 421 SKSGGRRRL 400

BLAST of Sed0010326 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 74.3 bits (181), Expect = 4.3e-13
Identity = 115/402 (28.61%), Postives = 183/402 (45.52%), Query Frame = 0

Query: 301 RKKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDSVVHSVAKAKERKLP------ 360
           R+K + G      ++ S T  +L  +  + S  I  P+ SV         +K P      
Sbjct: 118 RRKSVEGSVVIKVETPSSTRKSLGSVSRQ-SPGITKPDSSVSAKRDALAVKKKPCASVNS 177

Query: 361 EKSDKISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNS 420
           E S K     +  V+ L SV S   +R+NK+++ G S        KKV A+ T   S+++
Sbjct: 178 ESSSKEGSEIAKSVDGL-SVKSNDRARKNKETESGLS---GSAVVKKVPALRTDKSSTST 237

Query: 421 IDGTTNLPCINLKEVPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKT 480
             G++       K    +N   +E +K  Q                   T   E V+EKT
Sbjct: 238 GVGSS-------KVCAPKNLKNVEKAKTTQ-------------------TISGEDVKEKT 297

Query: 481 LYVIEIENEEIPHQPDQNETDDNMEPLPSSPPESLSLTASPHISADVKDQDVSEYTESEE 540
           + V+E   + +  +   +     M+    S  +SLS T     S   +         +++
Sbjct: 298 VCVVESSVKGVKSEKQPSSEKKTMK----SGNKSLSTTPKRGSSPTKQIPGKISTGLTKK 357

Query: 541 KEDSYSESNEMGSMKANDVSSEGGKTGGSQNLRLLKANEMDPQSTKLSFRRGKVIDIRSE 600
           KE   ++  E        V  +  KTG   +L           + +++F++GKV+D + E
Sbjct: 358 KETGSADVVEANPKPEKKVRPK--KTGVKVSL-----------AQQMTFKKGKVLDPKPE 417

Query: 601 NYSPRRLKFRPVRLLGESQSAGDGLRKNFKRGK-----EVDSNTTTAKETVVLRHHDVQE 660
           + SPR +KF+  R++ E ++  +G +KN K  +     + DS   + +E VVLRH  V+ 
Sbjct: 418 DSSPRWIKFKK-RVVQELKTQSEGKKKNLKDRRLGVETKTDSCEGSKREKVVLRHRKVEG 470

Query: 661 KKDAHGLYNNVIEETASKLVETRNSKVKALVGAFETVIFLQD 692
           KK    L+NNVIEET +KL + R  KVKAL+GAFETVI LQD
Sbjct: 478 KKKMITLFNNVIEETVNKLTKVRKHKVKALIGAFETVISLQD 470

BLAST of Sed0010326 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 58.5 bits (140), Expect = 2.4e-08
Identity = 109/406 (26.85%), Postives = 181/406 (44.58%), Query Frame = 0

Query: 302 KKKLLGDPRTSPKSRSHTENALKKLKPETSSAIRYPEDS--VVHSVAKAKERKLPEKSDK 361
           K++  G+   S       ++  K   P  S  +R  + +  VV+  ++  + K P K D 
Sbjct: 139 KREAAGEVVKSCDGLHVKKSETKSTSPSVSPVVRTVKKTNLVVNKASRISQNKSP-KEDL 198

Query: 362 ISKLKSIKVNPLRSVVSLINSRRNKDSKMGKSSGTSKVAAKKVVAVSTGSLSSNSIDGTT 421
              LK+ +   +   V   +     D ++ K S  S+  + K   +   +     ID   
Sbjct: 199 SKNLKNKEKGKIVEPVRCDDVLEKTDLEVKKVSRISENKSSKEDTLK--NKEKAKIDEPV 258

Query: 422 NLPCINLKEVPLENHNKIEISKCEQVRSEEVEGEIFRNEEVQGETFHSEKVQEKTLYVIE 481
               + L++  L+      IS+ +  + E ++    + +    E    +   EKTLYV+E
Sbjct: 259 RCDDV-LEKTSLDAQKVSRISENKNSKEERLKNLKNKEKTNIDEPVRPDDAVEKTLYVVE 318

Query: 482 IENEEIPHQPDQ-----NETDDNMEP--LPSSPPESLSLTAS-PHISADVKDQDVSEYTE 541
              E+   +        +ET  + E   + S+  +SLSL  S P  S  V   D     +
Sbjct: 319 SSVEKKKKKMSTKSVKISETQQSSEKKIIRSTGKKSLSLLPSLPPPSEVVTGSDPRPIRQ 378

Query: 542 SEEK-EDSYSESNEMGSMKANDVSSEGGKTG-GSQNLRLLKANEMDPQSTKLSFRRGKVI 601
           +  + + S  E  + GS  AN V++   ++    + + L       P   +++F++GKV+
Sbjct: 379 TTSRSKTSLPEKKQSGS--ANLVTNPKPESKIRPKRIGLKVTPPPPPTKQQMNFKKGKVL 438

Query: 602 DIRSENYSPRRLKFRPVRLLGESQSAGDGLRK----NFKRGKEVDSNTTTAKETVVLRHH 661
           + + E+ +   +KF+ + +        D  +K      KR      N    +E VVLRH 
Sbjct: 439 EPKPEDSTTTSIKFKKIVVQEPKLRTSDVNKKKKSLKDKREGVGKINGEGKREKVVLRHR 498

Query: 662 DVQEKKDAHGLYNNVIEETASKLVETRNSKVKALVGAFETVIFLQD 692
            V+ KK    L+NNVIEET +KL E R SKVKALVGAFETVI LQD
Sbjct: 499 KVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKALVGAFETVISLQD 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022990927.11.1e-19963.91uncharacterized protein LOC111487671 isoform X2 [Cucurbita maxima][more]
XP_023541286.11.1e-19962.18uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_023541278.11.1e-19962.18uncharacterized protein LOC111801497 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022990925.11.0e-19763.14uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima] >XP_022990926... [more]
XP_022990929.11.3e-19261.86uncharacterized protein LOC111487671 isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1JK935.2e-20063.91uncharacterized protein LOC111487671 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JPA74.9e-19863.14uncharacterized protein LOC111487671 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JTD56.1e-19361.86uncharacterized protein LOC111487671 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GZM45.7e-19158.70uncharacterized protein LOC111459082 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H1323.7e-19058.83uncharacterized protein LOC111459082 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G39380.18.9e-2733.60Plant calmodulin-binding protein-related [more]
AT5G15430.13.2e-1626.52Plant calmodulin-binding protein-related [more]
AT5G61260.14.3e-1328.61Plant calmodulin-binding protein-related [more]
AT5G07820.12.4e-0826.85Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 577..688
e-value: 1.2E-25
score: 101.2
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 580..687
e-value: 6.8E-32
score: 110.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..517
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 286..325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 286..332
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..143
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..158
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 201..695
coord: 14..171
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 201..695
coord: 14..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0010326.1Sed0010326.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding