Homology
BLAST of Sed0010232 vs. NCBI nr
Match:
XP_023525784.1 (DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo])
HSP 1 Score: 980.7 bits (2534), Expect = 5.3e-282
Identity = 482/528 (91.29%), Postives = 504/528 (95.45%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISKFRYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKFRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTG+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHFYLEDL+ASVEI+LSSAKITTGLFTENTIVVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRAVNDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIAA PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. NCBI nr
Match:
XP_022949454.1 (DNA polymerase epsilon subunit B [Cucurbita moschata])
HSP 1 Score: 976.5 bits (2523), Expect = 1.0e-280
Identity = 479/528 (90.72%), Postives = 504/528 (95.45%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISK+RYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+G+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHF+LEDL+ASVEI+LSSAKITTGLFTENTIVVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFFLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRAVNDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIAA PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. NCBI nr
Match:
XP_022998323.1 (DNA polymerase epsilon subunit B [Cucurbita maxima])
HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-280
Identity = 477/528 (90.34%), Postives = 504/528 (95.45%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISK+RYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+G+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHFYLEDL+ASVEI+LSSAKITTGLFTENT+VVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTVVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRA+NDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIA+ PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIASRPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. NCBI nr
Match:
XP_038894268.1 (DNA polymerase epsilon subunit B [Benincasa hispida])
HSP 1 Score: 961.4 bits (2484), Expect = 3.3e-276
Identity = 475/529 (89.79%), Postives = 499/529 (94.33%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIR-RFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSS 98
MD TLRKKVQRKAKIR + IKL+AL EIVSF SRF GCEDDAI+LVLDNLHDESL+SS
Sbjct: 1 MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGCEDDAIDLVLDNLHDESLKSS 60
Query: 99 IIDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGN 158
I+DKD VHRVVSI+LAAD+VGEES +T TSTSALCI++AFDISKFRYD IKKIF+ HTG
Sbjct: 61 ILDKDAVHRVVSIMLAADEVGEESPNTITSTSALCIIDAFDISKFRYDPIKKIFYLHTGK 120
Query: 159 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGR 218
LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFDTG+SHFGSCEISPI SLV +TGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGMSHFGSCEISPIQSLVGQTGR 180
Query: 219 KWVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVIT 278
KWVMGVISQMEDGHFYLEDL+ASVEI+LS+AKITTGLFTENTIVVAEGEMLV GIFQV+T
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLFTENTIVVAEGEMLVEGIFQVVT 240
Query: 279 CGFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEV 338
CGFPPLE+RD SLKLLAGQDFFGGGAL+KEETLRLADLEK+AVNDMFVILSD+WLDSEE
Sbjct: 241 CGFPPLEERDKSLKLLAGQDFFGGGALSKEETLRLADLEKKAVNDMFVILSDIWLDSEEA 300
Query: 339 MGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLK 398
MGKLETILDGFENVEVVPSLFVLMGNFCSRP NLAFNSFSSLR QFGKLG+MIAAHPRL
Sbjct: 301 MGKLETILDGFENVEVVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGRMIAAHPRLI 360
Query: 399 EHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 458
EHSKFLFIPGPDDAGPSTVLPRCALPK+ TEELQMHVPNAIFSSNPCRVRFYTQEIVFFR
Sbjct: 361 EHSKFLFIPGPDDAGPSTVLPRCALPKFLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 420
Query: 459 QDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPT 518
QDLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPT
Sbjct: 421 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 480
Query: 519 PHVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
PHVIVL DRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 PHVIVLADRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529
BLAST of Sed0010232 vs. NCBI nr
Match:
KAG7037068.1 (DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 958.0 bits (2475), Expect = 3.7e-275
Identity = 472/528 (89.39%), Postives = 496/528 (93.94%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISK+RYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+G+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHF+LEDL+A S KITTGLFTENTIVVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFFLEDLTA-------SHKITTGLFTENTIVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRAVNDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIAA PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 521
BLAST of Sed0010232 vs. ExPASy Swiss-Prot
Match:
Q500V9 (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV=1)
HSP 1 Score: 748.0 bits (1930), Expect = 7.6e-215
Identity = 368/523 (70.36%), Postives = 437/523 (83.56%), Query Frame = 0
Query: 45 RKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCED-DAIELVLDNLHDESLQSSIIDKDV 104
RKK+Q+K K R +++K DAL EI+ FA +F +D +AI+L+LDNL E+ +SS +D +
Sbjct: 8 RKKIQKKFKNRGYNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAES 67
Query: 105 VHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNLPIHGD 164
V +++ LL A + EE TTS S+L I++AF + KF YD +KK F++HT +LPIHG+
Sbjct: 68 VRGLINRLLGAHNAPEE---PTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHGE 127
Query: 165 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRKWVMGV 224
ASAK ALYR+RF+LLSQR+SR +HFS+PAFD +S F + EIS I SL+S+ GRKWVMGV
Sbjct: 128 ASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMGV 187
Query: 225 ISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITCGFPPL 284
ISQ+EDGHFYLEDLSASVEI LS AKITTG FTENTI++AEGEM V+GIFQVITCGFPPL
Sbjct: 188 ISQLEDGHFYLEDLSASVEIDLSKAKITTGFFTENTIILAEGEMQVNGIFQVITCGFPPL 247
Query: 285 EDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVMGKLET 344
EDRD +LK + DFFGGG LTKEE ++LADLE++AVND FVILSD+WLD EEVM KLET
Sbjct: 248 EDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLET 307
Query: 345 ILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKEHSKFL 404
+LDGFE+VE VPSLFV MGNFCSRP NL+F S+SSLR QFGKLG+MI HPRLKE+S+FL
Sbjct: 308 VLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFL 367
Query: 405 FIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYR 464
FIPGP+DAGPSTVLPRCALPKY TEEL+ +PNAIFSSNPCRV+FY QEIVFFRQDLLYR
Sbjct: 368 FIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYR 427
Query: 465 MRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTPHVIVL 524
MRRSCL+ PS+EET+DPF+HLV TI HQSHLCPLPL +QPIIWNYDH L LYPTPH IVL
Sbjct: 428 MRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALRLYPTPHTIVL 487
Query: 525 GDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
GD+S+Q+ K+ G TCFNPGSF+ D TFVAYRP QEVELSAL
Sbjct: 488 GDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526
BLAST of Sed0010232 vs. ExPASy Swiss-Prot
Match:
A7YWS7 (DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1)
HSP 1 Score: 303.1 bits (775), Expect = 6.4e-81
Identity = 184/534 (34.46%), Postives = 287/534 (53.75%), Query Frame = 0
Query: 44 LRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCE-DDAIELVLDNLHDESLQSSIIDKD 103
LR + K+R ++ +A+ + + E +DA+E ++D + + L S++I++
Sbjct: 6 LRSRALSAFKLRGLMLRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSNMIERS 65
Query: 104 VVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYD-HIKKIFHQHTGNLP-- 163
VV AA +S+D T I+ AFDI +F Y+ KK N P
Sbjct: 66 VVE-------AAVQECSQSVDETID-HIFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAP 125
Query: 164 -IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DTGISHFGSCEISPILSLVSK 223
+ G A KA L+R+R+ +L QR R + F+ P D S F I +L SK
Sbjct: 126 NLFGTARDKAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTSK 185
Query: 224 TGRKWVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQ 283
G V+G+I+Q+++G F+LED + ++++ LS A+ +GL+TE+ V+AEG +F
Sbjct: 186 IGDVIVLGMITQLKEGKFFLEDPTGTIQLDLSKAQFHSGLYTESCFVLAEG-WFEDQVFH 245
Query: 284 VITCGFPPLEDRDTSLKLLAGQDFFGGGALTKEET-LRLADLEKRAVNDMFVILSDVWLD 343
V GFPP E T+ +FFGG + T +T +L LE + MFV +SDVWLD
Sbjct: 246 VNAFGFPPTEPSSTTRAYYGNTNFFGGPSNTSVKTSAKLKQLEDENKDAMFVFISDVWLD 305
Query: 344 SEEVMGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAH 403
EV+ KL + G+ P+ F+L GNF S P+ N +L+ L +I +
Sbjct: 306 QVEVLEKLHIMFSGYS--PAPPTCFILCGNFSSAPYGK--NQVQALKDSLKTLADIICGY 365
Query: 404 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEI 463
P + + S+F+F+PGP+D G ++LPR + T E + VP ++F++NPCR+++ TQEI
Sbjct: 366 PNIHQSSRFVFVPGPEDPGFGSILPRPPFAESITNEFRQRVPFSVFTTNPCRIQYCTQEI 425
Query: 464 VFFRQDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLH 523
+ FR+DL+ +M R+C+ PS+ D H V T+ Q HL PLPL + P+ W YD+ L
Sbjct: 426 IVFREDLVNKMCRNCVRFPSS--NLDIPNHFVKTVLSQGHLTPLPLYVCPVYWAYDYALR 485
Query: 524 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSF-ANDCTFVAYRPFNQEVELSAL 567
+YP P ++V+ D+ T C NPGSF + +F + P N+ VE S L
Sbjct: 486 VYPVPDLLVIADKYDPFTLTNTECLCINPGSFPRSGFSFKVFYPSNKVVEDSKL 524
BLAST of Sed0010232 vs. ExPASy Swiss-Prot
Match:
Q5ZKQ6 (DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1)
HSP 1 Score: 302.4 bits (773), Expect = 1.1e-80
Identity = 183/539 (33.95%), Postives = 295/539 (54.73%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCE-DDAIELVLDNLHDESLQSS 98
M+ LR+++ ++R ++ +A+ + + E +D I+ ++D + + L ++
Sbjct: 1 MEPERLRRRLGSAFRVRGLLLRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLSTN 60
Query: 99 IIDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIF-HQHTG 158
+I+ +S + AA ++LD T + I+ AFDI ++ Y+ +K F
Sbjct: 61 MIE-------LSTVEAAVQECSQALDETIK-NVFNIIGAFDIPRYIYNPDRKRFVPLSMT 120
Query: 159 NLPI---HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DTGISHFGSCEISPIL 218
NLP+ G A KA L+R+R+ +L QR R + F+ A D S F + +L
Sbjct: 121 NLPVPNLFGTARDKAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKFQLKTVETLL 180
Query: 219 SLVSKTGRKWVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLV 278
+K V+G+I+Q+++G ++LED + V++ LS A+ +GL+TE+ V+AEG
Sbjct: 181 GNTAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKAQFHSGLYTESCFVLAEG-WYE 240
Query: 279 SGIFQVITCGFPPLEDRDTSLKLLAGQDFFGG-GALTKEETLRLADLEKRAVNDMFVILS 338
+F V GFPP E T+ +FFGG + + + + +L LE + MFV LS
Sbjct: 241 DEVFHVNAFGFPPTEPSATTRAFYGNINFFGGPSSASVKASAKLKQLEDENEDAMFVFLS 300
Query: 339 DVWLDSEEVMGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGK 398
DVWLD EV+ KL T+ G+ + P+ F GNF S P+ N SL+ L
Sbjct: 301 DVWLDQAEVLEKLHTMFSGYSSAP--PTCFFFCGNFSSAPYGK--NQIQSLKGSLKALAD 360
Query: 399 MIAAHPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRF 458
+I +P + + S+F+F+PGP+D GP +VLPR L + T+E + VP ++F++NPCRV++
Sbjct: 361 IICEYPSIHKSSRFVFVPGPEDPGPGSVLPRPPLAENITQEFRQLVPFSVFTTNPCRVQY 420
Query: 459 YTQEIVFFRQDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNY 518
TQEI+ FR+DL+ +M R+C+ PS+ + D H V TI Q HL PLPL + P+ W Y
Sbjct: 421 CTQEIIIFREDLVNKMCRNCVRFPSS--SMDIPNHFVKTILSQGHLTPLPLYVSPVYWAY 480
Query: 519 DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSF-ANDCTFVAYRPFNQEVELSAL 567
D+ L +YP P ++V+ D+ T C NPGSF + +F + P N+ VE S L
Sbjct: 481 DYALRVYPVPDMLVIADKYDPFTVTNTDCLCINPGSFPRSGFSFKVFYPSNKTVEDSKL 524
BLAST of Sed0010232 vs. ExPASy Swiss-Prot
Match:
Q54Y85 (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 PE=3 SV=1)
HSP 1 Score: 302.4 bits (773), Expect = 1.1e-80
Identity = 173/520 (33.27%), Postives = 286/520 (55.00%), Query Frame = 0
Query: 45 RKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSIIDKDVV 104
+K++ + + F IK DA+ I++ I+ +L NL +I +
Sbjct: 6 KKQITKSFSLTGFSIKADAMKCIINRIKNEGYDITQLIDNLLKNLDKSQYLQTI---EGC 65
Query: 105 HRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYD-HIKKIFHQHTGNLPIHGD 164
+++ + ++D + +E AL +++AF+ F +D + K+ + +HG
Sbjct: 66 FKLIDTINSSDAIEKE---------ALKVIDAFNTPLFNFDSNSKQFIKSENFDRSLHGS 125
Query: 165 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRKWVMGV 224
+++K+ LYR R+L + QR R+ +FS P + I+P+ SL+ TGRK V+G
Sbjct: 126 SNSKSDLYRKRYLKVLQRTERNDYFSTPVLASDKLKNEYQTITPLSSLLGNTGRKHVLGT 185
Query: 225 ISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITCGFPPL 284
ISQ+E+ FY+EDL+ +V+I +S AK G+ T N+I+ A GE + G+F PP
Sbjct: 186 ISQIEEDQFYIEDLNTNVKIDISKAKFEFGIVTINSIIQASGE-FIDGVFIADKIQLPPT 245
Query: 285 EDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVMGKLET 344
E+R +LK L D FG K+ +L EK ++ + LSDVWLDSE VM +L+
Sbjct: 246 EERCETLKFLQNIDMFGERP-QKKTMEQLIKYEKEKEDNSILFLSDVWLDSERVMERLDY 305
Query: 345 ILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKEHSKFL 404
+ G++ + P +LMGNF P L + L+ F +L +I +P + + ++F+
Sbjct: 306 LFGGYK--DCPPFAMILMGNFTEHP--LINGTQYQLKKYFNQLAMVIQKYPNIHQFTQFI 365
Query: 405 FIPGPDDAGPS--TVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLL 464
F+PGP D S +LP+ + F ++ +P + F++NPCR+R+ +QEI+ FR DL
Sbjct: 366 FVPGPTDPTGSLLNILPKFPISNVFIKDFISLIPKSTFTTNPCRIRYCSQEIIIFRDDLT 425
Query: 465 YRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTPHVI 524
+MRR C++ PS ++ D +HL+ I SHLC L L+ +PI WNYDH + +YP P ++
Sbjct: 426 NKMRRHCILEPS--QSCDISQHLIELICSNSHLCNLTLEDKPIYWNYDHAMSVYPLPDLL 485
Query: 525 VLGDRSKQ-QAFKYTGITCFNPGSFANDCTFVAYRPFNQE 561
V+GD+S Q + + G NP SF+ D +F Y P N++
Sbjct: 486 VIGDKSNQYEHSRADGTYSMNPSSFSTDYSFAHYIPANKQ 505
BLAST of Sed0010232 vs. ExPASy Swiss-Prot
Match:
P56282 (DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2)
HSP 1 Score: 294.7 bits (753), Expect = 2.3e-78
Identity = 183/534 (34.27%), Postives = 284/534 (53.18%), Query Frame = 0
Query: 44 LRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCE-DDAIELVLDNLHDESLQSSIIDKD 103
LR + K+R ++ +A+ + S E +D +E +++ + + L S++I++
Sbjct: 6 LRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERS 65
Query: 104 VVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYD-HIKKIFHQHTGNLP-- 163
VV AA +S+D T I+ AFDI +F Y+ KK N P
Sbjct: 66 VVE-------AAVQECSQSVDETIE-HVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAP 125
Query: 164 -IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DTGISHFGSCEISPILSLVSK 223
+ G KA ++R+R+ +L QR R + F+ P D S F I +L +K
Sbjct: 126 NLFGTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTK 185
Query: 224 TGRKWVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQ 283
G V+G+I+Q+++G F+LED + +V++ LS A+ +GL+TE V+AEG +F
Sbjct: 186 IGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEG-WFEDQVFH 245
Query: 284 VITCGFPPLEDRDTSLKLLAGQDFFGGGALTKEET-LRLADLEKRAVNDMFVILSDVWLD 343
V GFPP E T+ +FFGG + T +T +L LE+ + MFV LSDVWLD
Sbjct: 246 VNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLD 305
Query: 344 SEEVMGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAH 403
EV+ KL + G+ P+ F+L GNF S P+ N +L+ L +I +
Sbjct: 306 QVEVLEKLRIMFAGYS--PAPPTCFILCGNFSSAPYGK--NQVQALKDSLKTLADIICEY 365
Query: 404 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEI 463
P + + S+F+F+PGP+D G ++LPR L + T E + VP ++F++NPCR+++ TQEI
Sbjct: 366 PDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEI 425
Query: 464 VFFRQDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLH 523
FR+DL+ +M R+C+ PS+ H V TI Q HL PLPL + P+ W YD+ L
Sbjct: 426 TVFREDLVNKMCRNCVRFPSSNLAIP--NHFVKTILSQGHLTPLPLYVCPVYWAYDYALR 485
Query: 524 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSF-ANDCTFVAYRPFNQEVELSAL 567
+YP P ++V+ D+ T C NPGSF + +F + P N+ VE S L
Sbjct: 486 VYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKL 524
BLAST of Sed0010232 vs. ExPASy TrEMBL
Match:
A0A6J1GCV3 (DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=3 SV=1)
HSP 1 Score: 976.5 bits (2523), Expect = 4.8e-281
Identity = 479/528 (90.72%), Postives = 504/528 (95.45%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISK+RYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+G+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHF+LEDL+ASVEI+LSSAKITTGLFTENTIVVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFFLEDLTASVEINLSSAKITTGLFTENTIVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRAVNDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIAA PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAARPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. ExPASy TrEMBL
Match:
A0A6J1KGF0 (DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 SV=1)
HSP 1 Score: 976.1 bits (2522), Expect = 6.3e-281
Identity = 477/528 (90.34%), Postives = 504/528 (95.45%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MDE TLR+KVQRK+KIR + IKLDAL+EIVSFASRF GCEDDAI+LVLD+LHDESLQSSI
Sbjct: 1 MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
IDKD VHRVVSILLAAD+ GEE DT+TSTSALCI++AFDISK+RYD IKKIF+ HTG+L
Sbjct: 61 IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFD+G+SHFGSCEISPI SLV +TGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGRK 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHFYLEDL+ASVEI+LSSAKITTGLFTENT+VVAEGEMLV GIF+VITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLFTENTVVVAEGEMLVEGIFKVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLKLLAGQDFFGGG+LTKEETLRLADLEKRA+NDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
GKLETILDGFENVE+VPSLFVLMGNFCSRP NLAFNS+SSLR QFGKLGKMIA+ PRLKE
Sbjct: 301 GKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIASRPRLKE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ
Sbjct: 361 HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. ExPASy TrEMBL
Match:
A0A1S4DTQ5 (DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1)
HSP 1 Score: 933.7 bits (2412), Expect = 3.6e-268
Identity = 460/529 (86.96%), Postives = 490/529 (92.63%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIR-RFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSS 98
MD TLRKKVQRKAKIR + IKL+AL EIVSF SRF G EDDAIELVLD+LH+ESL+S
Sbjct: 1 MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
Query: 99 IIDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGN 158
I++KD VHRV+S LLAA++ E S +T TST+ALCI++AFDISKFRYD IKKIFH+HTG+
Sbjct: 61 ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120
Query: 159 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGR 218
LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD G+SHFGSCEISPI SLV +TGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
Query: 219 KWVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVIT 278
KWVMGVISQMEDGHFYLEDL+ASVEI+LS+AKITTGLFTENTI+VAEGEMLV GIFQV+T
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLFTENTIIVAEGEMLVEGIFQVVT 240
Query: 279 CGFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEV 338
CGFPPLE+RD SLKLLAGQDFFGGG L KEETLRLADLEK+AVNDMFVILSD+WLDSEE
Sbjct: 241 CGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLDSEEA 300
Query: 339 MGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLK 398
MGKLETILDGFENVEVVPSLFVLMGNFCS P N+AFNSFSSLR QFGKLGKMIAAHPRL
Sbjct: 301 MGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAHPRLN 360
Query: 399 EHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 458
+HSKF FIPGPDDAGPSTVLPRCALPKY TEELQ+HVPNA FSSNPCRVRFYTQEIVFFR
Sbjct: 361 KHSKFXFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEIVFFR 420
Query: 459 QDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPT 518
QDLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIWNYDHCLHLYPT
Sbjct: 421 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 480
Query: 519 PHVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
PHVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 481 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529
BLAST of Sed0010232 vs. ExPASy TrEMBL
Match:
A0A5D3BIF9 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00380 PE=3 SV=1)
HSP 1 Score: 928.3 bits (2398), Expect = 1.5e-266
Identity = 461/540 (85.37%), Postives = 492/540 (91.11%), Query Frame = 0
Query: 38 KMDEPTLRKKVQRKAKIR-RFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQS 97
+MD TLRKKVQRKAKIR + IKL+AL EIVSF SRF G EDDAIELVLD+LH+ESL+S
Sbjct: 612 RMDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKS 671
Query: 98 SIIDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTG 157
I++KD VHRV+S LLAA++ E S +T TST+ALCI++AFDISKFRYD IKKIFH+HTG
Sbjct: 672 PILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTG 731
Query: 158 NLPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTG 217
+LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD G+SHFGSCEISPI SLV +TG
Sbjct: 732 DLPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTG 791
Query: 218 RKWVMGVISQMEDGHFYLEDLSASVEISLSSA----------KITTGLFTENTIVVAEGE 277
RKWVMGVISQMEDGHFYLEDL+ASVEI+LS+A KITTGLFTENTI+VAEGE
Sbjct: 792 RKWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGE 851
Query: 278 MLVSGIFQVITCGFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVI 337
MLV GIFQV+TCGFPPLE+RD SLKLLAGQDFFGGG L KEETLRLADLEK+AVNDMFVI
Sbjct: 852 MLVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVI 911
Query: 338 LSDVWLDSEEVMGKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKL 397
LSD+WLDSEE MGKLETILDGFENVEVVPSLFVLMGNFCS P N+AFNSFSSLR QFGKL
Sbjct: 912 LSDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKL 971
Query: 398 GKMIAAHPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRV 457
GKMIAAHPRL +HSKFLFIPGPDDAGPSTVLPRCALPKY TEELQ+HVPNA FSSNPCRV
Sbjct: 972 GKMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRV 1031
Query: 458 RFYTQEIVFFRQDLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIW 517
RFYTQEIVFFRQDLLYRMRRSCL+PPSTEETSDPFEHLVATI HQSHLCPLPL IQPIIW
Sbjct: 1032 RFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIW 1091
Query: 518 NYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
NYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSF ND TFVAYRP NQEVELSAL
Sbjct: 1092 NYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1151
BLAST of Sed0010232 vs. ExPASy TrEMBL
Match:
A0A6J1C7G4 (DNA polymerase epsilon subunit OS=Momordica charantia OX=3673 GN=LOC111009082 PE=3 SV=1)
HSP 1 Score: 927.9 bits (2397), Expect = 2.0e-266
Identity = 455/528 (86.17%), Postives = 490/528 (92.80%), Query Frame = 0
Query: 39 MDEPTLRKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCEDDAIELVLDNLHDESLQSSI 98
MD T+R+KVQRK+KIR + +KLDAL EI+SFASRF G ED AI+ +LD LH ESLQSSI
Sbjct: 1 MDASTMRRKVQRKSKIRGYSLKLDALDEILSFASRFHGEEDAAIDFILDELHSESLQSSI 60
Query: 99 IDKDVVHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNL 158
I KD VHR VS+LL AD+VG++S DT TSTSA I++ F I++FRYD IKKIF+QHTG+L
Sbjct: 61 IHKDAVHRAVSVLLGADEVGKQSPDTITSTSAPRIIDVFGITRFRYDPIKKIFYQHTGSL 120
Query: 159 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRK 218
PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF+TG+SHFGSCEIS I SLV +TGR+
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFNTGMSHFGSCEISSIQSLVGQTGRR 180
Query: 219 WVMGVISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITC 278
WVMGVISQMEDGHFYLEDL+ASVEI+LS+AKITTGLFTENTIVVAEGEMLV GIFQVITC
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLFTENTIVVAEGEMLVEGIFQVITC 240
Query: 279 GFPPLEDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVM 338
GFPPLEDRD SLK+LAGQDFFG GALTKEETLRLADLEKRA+NDMFVILSD+WLDSEE M
Sbjct: 241 GFPPLEDRDKSLKMLAGQDFFGAGALTKEETLRLADLEKRAINDMFVILSDIWLDSEEAM 300
Query: 339 GKLETILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKE 398
KLETILDGFENVEVVPSLFVLMGNFCSRP NLAFNSFSSLR QFGKLGKMIAAHPRL E
Sbjct: 301 RKLETILDGFENVEVVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLIE 360
Query: 399 HSKFLFIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQ 458
+SKFLFIPGPDDAGPSTVLPRCALPKY TEEL+MHVPNAIFSSNPCR++FYTQEIVFFRQ
Sbjct: 361 NSKFLFIPGPDDAGPSTVLPRCALPKYLTEELRMHVPNAIFSSNPCRIKFYTQEIVFFRQ 420
Query: 459 DLLYRMRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTP 518
DLLYRMRRSCL+PPSTEETSDPFEHL+ATI HQSHLCPLPL IQPIIWNYDHCLHLYPTP
Sbjct: 421 DLLYRMRRSCLIPPSTEETSDPFEHLIATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTP 480
Query: 519 HVIVLGDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
HVIVLGDRSKQQAFKYTGITCFNPGSF+ND TFVAYRP NQEVELSAL
Sbjct: 481 HVIVLGDRSKQQAFKYTGITCFNPGSFSNDGTFVAYRPCNQEVELSAL 528
BLAST of Sed0010232 vs. TAIR 10
Match:
AT5G22110.1 (DNA polymerase epsilon subunit B2 )
HSP 1 Score: 748.0 bits (1930), Expect = 5.4e-216
Identity = 368/523 (70.36%), Postives = 437/523 (83.56%), Query Frame = 0
Query: 45 RKKVQRKAKIRRFDIKLDALSEIVSFASRFSGCED-DAIELVLDNLHDESLQSSIIDKDV 104
RKK+Q+K K R +++K DAL EI+ FA +F +D +AI+L+LDNL E+ +SS +D +
Sbjct: 8 RKKIQKKFKNRGYNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAES 67
Query: 105 VHRVVSILLAADDVGEESLDTTTSTSALCIVNAFDISKFRYDHIKKIFHQHTGNLPIHGD 164
V +++ LL A + EE TTS S+L I++AF + KF YD +KK F++HT +LPIHG+
Sbjct: 68 VRGLINRLLGAHNAPEE---PTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHGE 127
Query: 165 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGISHFGSCEISPILSLVSKTGRKWVMGV 224
ASAK ALYR+RF+LLSQR+SR +HFS+PAFD +S F + EIS I SL+S+ GRKWVMGV
Sbjct: 128 ASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMGV 187
Query: 225 ISQMEDGHFYLEDLSASVEISLSSAKITTGLFTENTIVVAEGEMLVSGIFQVITCGFPPL 284
ISQ+EDGHFYLEDLSASVEI LS AKITTG FTENTI++AEGEM V+GIFQVITCGFPPL
Sbjct: 188 ISQLEDGHFYLEDLSASVEIDLSKAKITTGFFTENTIILAEGEMQVNGIFQVITCGFPPL 247
Query: 285 EDRDTSLKLLAGQDFFGGGALTKEETLRLADLEKRAVNDMFVILSDVWLDSEEVMGKLET 344
EDRD +LK + DFFGGG LTKEE ++LADLE++AVND FVILSD+WLD EEVM KLET
Sbjct: 248 EDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLET 307
Query: 345 ILDGFENVEVVPSLFVLMGNFCSRPFNLAFNSFSSLRSQFGKLGKMIAAHPRLKEHSKFL 404
+LDGFE+VE VPSLFV MGNFCSRP NL+F S+SSLR QFGKLG+MI HPRLKE+S+FL
Sbjct: 308 VLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFL 367
Query: 405 FIPGPDDAGPSTVLPRCALPKYFTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYR 464
FIPGP+DAGPSTVLPRCALPKY TEEL+ +PNAIFSSNPCRV+FY QEIVFFRQDLLYR
Sbjct: 368 FIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYR 427
Query: 465 MRRSCLMPPSTEETSDPFEHLVATIAHQSHLCPLPLDIQPIIWNYDHCLHLYPTPHVIVL 524
MRRSCL+ PS+EET+DPF+HLV TI HQSHLCPLPL +QPIIWNYDH L LYPTPH IVL
Sbjct: 428 MRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALRLYPTPHTIVL 487
Query: 525 GDRSKQQAFKYTGITCFNPGSFANDCTFVAYRPFNQEVELSAL 567
GD+S+Q+ K+ G TCFNPGSF+ D TFVAYRP QEVELSAL
Sbjct: 488 GDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_023525784.1 | 5.3e-282 | 91.29 | DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo] | [more] |
XP_022949454.1 | 1.0e-280 | 90.72 | DNA polymerase epsilon subunit B [Cucurbita moschata] | [more] |
XP_022998323.1 | 1.3e-280 | 90.34 | DNA polymerase epsilon subunit B [Cucurbita maxima] | [more] |
XP_038894268.1 | 3.3e-276 | 89.79 | DNA polymerase epsilon subunit B [Benincasa hispida] | [more] |
KAG7037068.1 | 3.7e-275 | 89.39 | DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
Q500V9 | 7.6e-215 | 70.36 | DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV... | [more] |
A7YWS7 | 6.4e-81 | 34.46 | DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1 | [more] |
Q5ZKQ6 | 1.1e-80 | 33.95 | DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1 | [more] |
Q54Y85 | 1.1e-80 | 33.27 | DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 P... | [more] |
P56282 | 2.3e-78 | 34.27 | DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GCV3 | 4.8e-281 | 90.72 | DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=... | [more] |
A0A6J1KGF0 | 6.3e-281 | 90.34 | DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 ... | [more] |
A0A1S4DTQ5 | 3.6e-268 | 86.96 | DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1 | [more] |
A0A5D3BIF9 | 1.5e-266 | 85.37 | Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
A0A6J1C7G4 | 2.0e-266 | 86.17 | DNA polymerase epsilon subunit OS=Momordica charantia OX=3673 GN=LOC111009082 PE... | [more] |
Match Name | E-value | Identity | Description | |
AT5G22110.1 | 5.4e-216 | 70.36 | DNA polymerase epsilon subunit B2 | [more] |