Sed0010171 (gene) Chayote v1

Overview
NameSed0010171
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG11: 30779781 .. 30782136 (+)
RNA-Seq ExpressionSed0010171
SyntenySed0010171
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGGGAAGGTGGTCGTTTCGGGAATGTCCCTTATTCTTGTCGTTGGTGTGGCTCTCGCTGTCGTCGCTGTCATTAACAAGGGAAATAAATCCGAGGCCATGGACGACCTGTCGCCAAAGATGAAGTCAGTCCAAACCATATGTATGGGTACGGATTACAAAAAGGAATGCCAGAATACTCTTGCTAACATTGCTCATAATGCCTCTTCAGATGATCCAAGAGAGTATGTTAAAGCTGCAATTCTTGCAACTATGGATGAGGTTACGAAAGGCTTGAACTTGACCGATACGCTTATGGTCGAGGCTAAAAACAATGCTTCCATCAAAATGTCTGTTGATGATTGCAAGGACCTATTGCAGTTCGCCATCGAATCCTTGCAAGCATCTTACTCCACTGTCGGGGACCCTAATGTGCACACCGATGCAGATCGCATCGATGATCTCAAGACTTGGCTCAGCTCCGTCGTTTCCTTCCAAGAATCTTGCAAGGATGGGCTTGGAGAATTCGATGTCGAACTCAAACAGAAAATGCAAGATGGTCTCGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCGTAGATGAAGTTTCCGAACTTCTCGAAAGCATCGGCATGCAATTGAAGGTTCCGCTAAATCACTAACTAAACTCCATAAGTTTATTTGATAGGTTATAGTACATTTGAATCCTATACATATTTTCTTTTGTCACCAGGTCAAACCAGCCAACCGTAAGCTTCTTGGAGCAACAGAAGTAGGAAGGGACGGCATTCCGATATGGCTCTCGGGGGGAGACAGGAGGCTGCTGGCGGGAGCGAGAGGAGGCTCGGCCCAAGTGAAGCCAAATGTCGTGGTGGCTAAGGACGGTAGCGGGCAGTTCAAGACCATTAATGCTGCATTGAATGCATACCCAAAGAACCTGAGGGGAAGATATGTAATCTACGTGAAAGCGGGGATCTATAACGAGTACATCATTATAACTAAAGAGAAGGTCAACATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAGAGCAACCGTGATGGCGTCACCACCCAGAACTCTGCCTCTTTCGGTAACTATTACAAACTCTATCATAAAATATTACGGTTTGACATTCTAAAATCTGACTGCCGCCATATATTGATCATTCGAGTATGTCTAGTTTCACGAAGAAATGAAATTGTTTCTTATCCAAAAATAAACTAAATTGATCACTAATGGGGCACGATGTGCACTCCCCGTAACACTCAAAGCTCATCTTGTTTGCACTTTTCAAGGACTTTGCTAAGACATTTTTTAACCCTCTTCAATTTCTTTTTTTTTCTTCTTTAAAATCATAAACCTCTCTTCCTTCTCACGCAAGATTTATTTCGCTACGAGTAGGAACGAAACCTTGCTTCGAAGATATACAGATGAATATTGAAACGAATGTGATTAATATATATGTTTTGTGGTATATAAGTATTATAAATGTTTTGTGCCTTTTTGCTCAAGCCCAAGGCTAACTCCAATGAACGAATCATTCAAATATGTCTAAACTATCAACGGATTTCGAAAATTTCTTTATGCTAGCCTCACGAAATCACTAAATTGTTCAGAAACTAACACAATTGGCACTACAAAATTACCTCGCAGCGGTCATCGGCGCTGGATTCCTCTGCAAATCCATGGGATTCCAAAACACGGCCGGTCCGGAAGGTCACCAAGCAGTAGCCCTAAGAAGCCAATCCGAAAAATCGGCATTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTATACGTGCAAGCCAACCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAAACACCATAATCCAAAACTCCTTGATCATCGTCCGCCGCCCCATGGACAACCAACAGAACACCGTGACCGCCAACGGCCGCAAGTACAGGCAGGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCGGAGCAGAAGCTGTTCGCCGAGCGGTTGAAGATCCCGACGTACCTGGGGCGGCCATGGAAGGAACACGCGAGAACGGTGATCATGGAGTCGACATTGGGGGATCTGATTCAGCCGGCGGGGTACCTGCCGTGGGCCGGGAGCTTCGCGTTGAGCACTTGCCAGTACTTGGAGTACGCGAACAAAGGACCGGGCGCGAACACGAACAGGAGAGTGCGATGGAAGGGCGTGAGAGTGATCGGAAGGAACGAGGCGTTGCAGTTCACGGCGGCGCCGTTCCTTCTCGGAAAAACTTGGCTTCAAGGCACCGGCGGCCAGTACTTCATGGGTTTGAGGAACTGA

mRNA sequence

ATGGTAGGGAAGGTGGTCGTTTCGGGAATGTCCCTTATTCTTGTCGTTGGTGTGGCTCTCGCTGTCGTCGCTGTCATTAACAAGGGAAATAAATCCGAGGCCATGGACGACCTGTCGCCAAAGATGAAGTCAGTCCAAACCATATGTATGGGTACGGATTACAAAAAGGAATGCCAGAATACTCTTGCTAACATTGCTCATAATGCCTCTTCAGATGATCCAAGAGAGTATGTTAAAGCTGCAATTCTTGCAACTATGGATGAGGTTACGAAAGGCTTGAACTTGACCGATACGCTTATGGTCGAGGCTAAAAACAATGCTTCCATCAAAATGTCTGTTGATGATTGCAAGGACCTATTGCAGTTCGCCATCGAATCCTTGCAAGCATCTTACTCCACTGTCGGGGACCCTAATGTGCACACCGATGCAGATCGCATCGATGATCTCAAGACTTGGCTCAGCTCCGTCGTTTCCTTCCAAGAATCTTGCAAGGATGGGCTTGGAGAATTCGATGTCGAACTCAAACAGAAAATGCAAGATGGTCTCGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCGTAGATGAAGTTTCCGAACTTCTCGAAAGCATCGGCATGCAATTGAAGGTCAAACCAGCCAACCGTAAGCTTCTTGGAGCAACAGAAGTAGGAAGGGACGGCATTCCGATATGGCTCTCGGGGGGAGACAGGAGGCTGCTGGCGGGAGCGAGAGGAGGCTCGGCCCAAGTGAAGCCAAATGTCGTGGTGGCTAAGGACGGTAGCGGGCAGTTCAAGACCATTAATGCTGCATTGAATGCATACCCAAAGAACCTGAGGGGAAGATATGTAATCTACGTGAAAGCGGGGATCTATAACGAGTACATCATTATAACTAAAGAGAAGGTCAACATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAGAGCAACCGTGATGGCGTCACCACCCAGAACTCTGCCTCTTTCGCGGTCATCGGCGCTGGATTCCTCTGCAAATCCATGGGATTCCAAAACACGGCCGGTCCGGAAGGTCACCAAGCAGTAGCCCTAAGAAGCCAATCCGAAAAATCGGCATTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTATACGTGCAAGCCAACCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAAACACCATAATCCAAAACTCCTTGATCATCGTCCGCCGCCCCATGGACAACCAACAGAACACCGTGACCGCCAACGGCCGCAAGTACAGGCAGGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCGGAGCAGAAGCTGTTCGCCGAGCGGTTGAAGATCCCGACGTACCTGGGGCGGCCATGGAAGGAACACGCGAGAACGGTGATCATGGAGTCGACATTGGGGGATCTGATTCAGCCGGCGGGGTACCTGCCGTGGGCCGGGAGCTTCGCGTTGAGCACTTGCCAGTACTTGGAGTACGCGAACAAAGGACCGGGCGCGAACACGAACAGGAGAGTGCGATGGAAGGGCGTGAGAGTGATCGGAAGGAACGAGGCGTTGCAGTTCACGGCGGCGCCGTTCCTTCTCGGAAAAACTTGGCTTCAAGGCACCGGCGGCCAGTACTTCATGGGTTTGAGGAACTGA

Coding sequence (CDS)

ATGGTAGGGAAGGTGGTCGTTTCGGGAATGTCCCTTATTCTTGTCGTTGGTGTGGCTCTCGCTGTCGTCGCTGTCATTAACAAGGGAAATAAATCCGAGGCCATGGACGACCTGTCGCCAAAGATGAAGTCAGTCCAAACCATATGTATGGGTACGGATTACAAAAAGGAATGCCAGAATACTCTTGCTAACATTGCTCATAATGCCTCTTCAGATGATCCAAGAGAGTATGTTAAAGCTGCAATTCTTGCAACTATGGATGAGGTTACGAAAGGCTTGAACTTGACCGATACGCTTATGGTCGAGGCTAAAAACAATGCTTCCATCAAAATGTCTGTTGATGATTGCAAGGACCTATTGCAGTTCGCCATCGAATCCTTGCAAGCATCTTACTCCACTGTCGGGGACCCTAATGTGCACACCGATGCAGATCGCATCGATGATCTCAAGACTTGGCTCAGCTCCGTCGTTTCCTTCCAAGAATCTTGCAAGGATGGGCTTGGAGAATTCGATGTCGAACTCAAACAGAAAATGCAAGATGGTCTCGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCGTAGATGAAGTTTCCGAACTTCTCGAAAGCATCGGCATGCAATTGAAGGTCAAACCAGCCAACCGTAAGCTTCTTGGAGCAACAGAAGTAGGAAGGGACGGCATTCCGATATGGCTCTCGGGGGGAGACAGGAGGCTGCTGGCGGGAGCGAGAGGAGGCTCGGCCCAAGTGAAGCCAAATGTCGTGGTGGCTAAGGACGGTAGCGGGCAGTTCAAGACCATTAATGCTGCATTGAATGCATACCCAAAGAACCTGAGGGGAAGATATGTAATCTACGTGAAAGCGGGGATCTATAACGAGTACATCATTATAACTAAAGAGAAGGTCAACATTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAGAGCAACCGTGATGGCGTCACCACCCAGAACTCTGCCTCTTTCGCGGTCATCGGCGCTGGATTCCTCTGCAAATCCATGGGATTCCAAAACACGGCCGGTCCGGAAGGTCACCAAGCAGTAGCCCTAAGAAGCCAATCCGAAAAATCGGCATTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTATACGTGCAAGCCAACCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAAACACCATAATCCAAAACTCCTTGATCATCGTCCGCCGCCCCATGGACAACCAACAGAACACCGTGACCGCCAACGGCCGCAAGTACAGGCAGGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCGGAGCAGAAGCTGTTCGCCGAGCGGTTGAAGATCCCGACGTACCTGGGGCGGCCATGGAAGGAACACGCGAGAACGGTGATCATGGAGTCGACATTGGGGGATCTGATTCAGCCGGCGGGGTACCTGCCGTGGGCCGGGAGCTTCGCGTTGAGCACTTGCCAGTACTTGGAGTACGCGAACAAAGGACCGGGCGCGAACACGAACAGGAGAGTGCGATGGAAGGGCGTGAGAGTGATCGGAAGGAACGAGGCGTTGCAGTTCACGGCGGCGCCGTTCCTTCTCGGAAAAACTTGGCTTCAAGGCACCGGCGGCCAGTACTTCATGGGTTTGAGGAACTGA

Protein sequence

MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQNTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQDGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRRLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQGTGGQYFMGLRN
Homology
BLAST of Sed0010171 vs. NCBI nr
Match: XP_022990080.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 891.0 bits (2301), Expect = 5.5e-255
Identity = 442/568 (77.82%), Postives = 505/568 (88.91%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGN-KSEAMDDLSPKMKSVQTICMGTDYKKECQ 60
           MVGKVVVSG+S ILVVGVALAVVAV+NK N  +  M  LSPKMK+V +IC GT+Y KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           +TL+  A N++SDDP++Y+KAAI+AT++E+TKGLNLTDTL VEA +N++I+MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQ AI+SL AS STVGDP++HTDADR+ D+KTWLS+V+SFQ+SC DGL E D +L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
             LD AGKLTSNALAI+DEV  +LE +G+QLKV+P+ R+LLG TEVG DG P WL G DR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA ARGG+ +VKPN VVAKDGSGQFKTI AAL AYPK LRGRYVIYVKAG+YNEYII+
Sbjct: 241 KLLA-ARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK   NIFMYGDGPR TIVTGRKSNRDGVTTQ+SASF+ IG GFLCKSMGF NTAGPEGH
Sbjct: 301 TKNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS++SAFFNCRMDG+QDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQ+NTVTANGR   +EN+GLVIHNCRIVPEQKLFA+R KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQKNTVTANGRADNRENTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGDLIQPAGY+PWAG+FAL TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA
Sbjct: 481 TVIMESTLGDLIQPAGYMPWAGNFALDTCLYLEYGNRGPGAITNRRVKWKGVRVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G TWLQGTGG +F+GL+N
Sbjct: 541 MQFTAEPFLQGSTWLQGTGGPFFVGLKN 567

BLAST of Sed0010171 vs. NCBI nr
Match: XP_023527159.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.8 bits (2298), Expect = 1.2e-254
Identity = 441/568 (77.64%), Postives = 504/568 (88.73%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGN-KSEAMDDLSPKMKSVQTICMGTDYKKECQ 60
           MVGKVVVSG+S ILVVGVALAVVAV+NK N  +  M  LSPKMK+V +IC GTDY KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           +TL+  A N++SDDP++Y+KAAI+AT++E+TKGLNLTDTL VEA +N++I+MSVDDCKDL
Sbjct: 61  STLSATAQNSTSDDPKQYIKAAIVATIEEITKGLNLTDTLRVEAADNSTIQMSVDDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQ AI+SL AS STVGDP++HTDADR+ D+KTWLS+V+SFQ+SC DGL E D +L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
            GLD AGKLTSNALAI+DEV  +LE +G+QLKV+P+ R+LLG TEVG DG P WL G DR
Sbjct: 181 GGLDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA ARGG+ +VKPN VVAKDGSGQFKTI AAL AYPK L GRYVIYVKAG+Y+EYII+
Sbjct: 241 KLLA-ARGGTGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK   NIFMYGDGPR TIVTGRKSNRDGVTTQ+SASF+ IG GFLCKSMGF NTAGPEGH
Sbjct: 301 TKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS+KSAFFNCRMDG+QDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI
Sbjct: 361 QAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFA+R KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGDLIQ AGY+PWAG+FAL TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA
Sbjct: 481 TVIMESTLGDLIQAAGYMPWAGNFALETCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G TWLQGTGG +F+GL+N
Sbjct: 541 MQFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Sed0010171 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 888.3 bits (2294), Expect = 3.6e-254
Identity = 433/567 (76.37%), Postives = 500/567 (88.18%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           MVGKVV+SG+SLILVVGVALAVVA++NK + S+   +LSPKMK+V +IC   DY+ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLL 120
           TL ++ +N+S +DP+E++KAAI +T++E+ KG NLTD LMVEA NNA+IKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQD 180
           Q AI+ L ASYSTVGDP++HT+ DRI D+K WL++V+S+Q+SC DGL EFD +LKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRR 240
           GLDVAGKLTSNALAIV  VS++L+ +G+QLKV+P+ R+LLG TEV  D  P WL+G DR+
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIIT 300
           LLA  RGG  +VKPNVVVAKDGSGQ+KTI AAL AYPK L+GRYVIYVKAG+YNE II+T
Sbjct: 241 LLAAKRGG-VRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQ 360
           K+  NIFMYGDGPRKTIVTGRKSNRDG+TTQN+ASFA IG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QS++SAFFNCRMDGYQDTLYVQ +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHART 480
           VRRPMDNQQNTVTANGR  ++E SGLVIHNCRIVPEQKLFAER KIPT+LGRPWKE+ART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGD IQPAGY+PW+G+FAL+TC Y EY N+GPGANTNRRVRWKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           QFTA PFLLGK WL GTGG Y +GL+N
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Sed0010171 vs. NCBI nr
Match: XP_022956875.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 887.5 bits (2292), Expect = 6.1e-254
Identity = 440/568 (77.46%), Postives = 502/568 (88.38%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEA-MDDLSPKMKSVQTICMGTDYKKECQ 60
           M+GKVVVSG+S ILVVGVALAVVAV+NK N + A M  LSPKMK+V  IC GTDY KECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           +TL+  A N++SDDP++Y+KAAI+AT++E+TKGLNLTDTL VEA +N +I+MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQ AI+SL AS STVGDP++HTDADR+ D+KTWLS+V+SFQ+SC DGL E D +L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
             LD AGKLTSNALAI+DEV  +LE +G+QLKV+P+ R+LLG TEVG DG P WL G DR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA ARGG+ +VKPN VVAKDGSGQFKTI AAL AYPK L GRYVIYVKAG+Y+EYII+
Sbjct: 241 KLLA-ARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK   NIFMYGDGPR TIVTGRKSNRDGVTTQ+SASF+ IG GFLCKSMGF NTAGPEGH
Sbjct: 301 TKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS+KSAFFNCRMDG+QDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI
Sbjct: 361 QAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFA+R KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGDLIQP GY+PWAG+FAL TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA
Sbjct: 481 TVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G TWLQGTGG +F+GL+N
Sbjct: 541 MQFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Sed0010171 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 882.9 bits (2280), Expect = 1.5e-252
Identity = 430/567 (75.84%), Postives = 501/567 (88.36%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           MVGKVVVSG+SLILVVGVALAVVA++ K N S+ +D  SPKMK+V TIC  TDY++ECQN
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSD-VDLSSPKMKAVSTICSTTDYQQECQN 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLL 120
           TL N+A NAS DDP+EY KAA+LAT+ E+TKG NLT  LMVEA NNASIKMSVDDCKDLL
Sbjct: 61  TLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLL 120

Query: 121 QFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQD 180
           Q AI+ L ASYSTVGDP++HT+ DR+ D+K WL++V+S+Q+SC DGL EFD +LKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRR 240
           GLDVAGKLTSNALAIV  VS++L++ G+QLKV+P+ R+LL  TE+G DG P W+SG DR+
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRK 240

Query: 241 LLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIIT 300
           LLA +RGG A+VKPN VVAKDGSGQ+K+I AAL AYPK LRGRYVIYVKAGIY+EYI +T
Sbjct: 241 LLA-SRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQ 360
           K+  N+FMYGDGPRKTIVTGRK+NRDG+TTQN+ASF+ IG GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQ 360

Query: 361 AVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QS++SAF+NCRMDGYQDTLYVQ +RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHART 480
           VRRPMDNQ+NTVTANGR  ++E SGLVIHNCRIVPEQKLFAER KIPT+LGRPWK++ART
Sbjct: 421 VRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIME+TLGD IQP GY+PW+G+FAL TC Y EY N+GPGANTNRRVRWKGVRVIGRNEAL
Sbjct: 481 VIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540

Query: 541 QFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           Q+TA PFL+G+ WL  TGG Y +GL++
Sbjct: 541 QYTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Sed0010171 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.6e-126
Identity = 267/603 (44.28%), Postives = 360/603 (59.70%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA INK   +    +LSP+MK+VQ IC  T  K  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA----NLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNAS--IKMSVDDCKD 120
           TL  +     S+DP + +KA +LAT DE+TK  N T    V   ++ S   K  +D CK 
Sbjct: 61  TLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKM 180
           +  +A+E L      +G+ ++     +ID LK WL  V ++Q  C D + E D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVDEVSELLESIGMQL--------------------------KV 240
            +G+  +  LT+NA+ I   V   +  I  ++                            
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 KPANRKLLGATEVGRDGIPIWLSGGDRRLLA-------GARGGSAQVKPNVVVAKDGSGQ 300
            PA R L    ++   GIP W+SG DR+L+A       G RGG A+V+ N VVAKDGSGQ
Sbjct: 241 GPARRLL---EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVTGRKS-- 360
           FKT+  A++A P+N RGR +IY+KAG+Y E +II K+K NIFM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCRMDGYQD 420
              G TT  SA+  V   GF+ K MGF+NTAGP GHQA A+R   +++  FNCR DGYQD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKY-RQE 480
           TLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G +     
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 NSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGYLPWAGS 540
             G+V+ NCRIVP++KL  ERL + TYLGRPWK+ + TVIM + +GDLI+P G+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQGTGGQYF 566
               +C+Y+EY N+GPGA  NRRV W  V      E   FTAA +L    W+Q       
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVT 588

BLAST of Sed0010171 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.8e-123
Identity = 243/527 (46.11%), Postives = 330/527 (62.62%), Query Frame = 0

Query: 43  KSVQTICMGTDYKKECQNTLAN-IAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMV 102
           K +QT+C  T YK  CQNTL N    +    DPR  +K+AI+A  D++ +      +L  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 103 EAKNNASIKMSVDDCKDLLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQE 162
           E K++   K ++  CK L+  A E L  S   + D  V+  A  + DL +WLS+V+S+QE
Sbjct: 153 ENKDD---KDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 163 SCKDGLGEFDVELKQKMQDGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLG 222
           +C DG  E   +LK +++   + +  LTSN+LA++  +   L S+    KVK   R LL 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KVK--TRLLLE 272

Query: 223 ATEVGR--DGIPIWLSGGDRRLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKN 282
           A    +  D I  WLS  +RR+L         +KPN  VAKDGSG F TINAAL A P  
Sbjct: 273 ARSSAKETDHITSWLSNKERRMLKAV--DVKALKPNATVAKDGSGNFTTINAALKAMPAK 332

Query: 283 LRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVI 342
            +GRY IY+K GIY+E +II K+K N+ M GDG +KTIVTG KS+   + T  +A+F   
Sbjct: 333 YQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQ 392

Query: 343 GAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVI 402
           G GF+ +SMGF+NTAGPEGHQAVA+R QS++S F NCR +GYQDTLY   +RQ+YR+CVI
Sbjct: 393 GEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVI 452

Query: 403 SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKL 462
            GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR  + + +G VIHNC + P + L
Sbjct: 453 IGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDL 512

Query: 463 FAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGYLPWAGS-FALSTCQYLEYANKGP 522
              + +  +YLGRPWK H+RTV+MEST+ D+I P G+L W  + FA+ T  Y EY N GP
Sbjct: 513 KPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGP 572

Query: 523 GANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQGTGGQYFMGL 566
              T  RV+W G RV+ + EA++FT  PFL G+ W+Q  G    +GL
Sbjct: 573 SGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Sed0010171 vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 434.1 bits (1115), Expect = 2.4e-120
Identity = 257/609 (42.20%), Postives = 359/609 (58.95%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA INK   +    +LSP+MK+V+ IC  T  K  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA----NLSPQMKAVRGICEATSDKASCVK 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNAS--IKMSVDDCKD 120
           TL  +     SDDP + +KA +LAT D +T+  N T        +  S   K  +D CK 
Sbjct: 61  TLEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCKK 120

Query: 121 LLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKM 180
           +  +A+E L      +G+ +++    +ID LK WL+ V ++Q  C D + E D  L++ +
Sbjct: 121 VFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKTI 180

Query: 181 QDGLDVAGKLTSNALAIVDEVSELLESIGMQLK----------------VKPAN------ 240
            +G+  +  LTSNA+ I   V   +  + ++++                  P N      
Sbjct: 181 GEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPPV 240

Query: 241 -------------RKLLGATEVGRDGIPIWLSGGDRRLLA----GARGGSAQVKPNVVVA 300
                        R+LL   ++   GIP W+SG DR+L+     G+  G A+++   VVA
Sbjct: 241 ADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVVA 300

Query: 301 KDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVT 360
           KDGSGQFKT+  A+NA P+   GR +I++KAGIY E +II K+K NIFM+GDG RKT+++
Sbjct: 301 KDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVIS 360

Query: 361 GRKSNR--DGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCR 420
             +S +   G TT  S +  V   GF+ K +GF+NTAGP GHQAVA+R   +++  FNCR
Sbjct: 361 YNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNCR 420

Query: 421 MDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANG- 480
            DGYQDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA+G 
Sbjct: 421 FDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTADGN 480

Query: 481 RKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGY 540
            K      G+V+ NCRIVP++KL AERL + +YLGRPWK+ + TVI+ S +GD+I+P G+
Sbjct: 481 EKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRPEGW 540

Query: 541 LPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQG 566
             W G     +C+Y+EY N+GPGA TNRRV W  +      E   FT A +L    W+Q 
Sbjct: 541 KIWDGESFHKSCRYVEYNNRGPGAITNRRVNWVKI-ARSAAEVNDFTVANWLGPINWIQE 595

BLAST of Sed0010171 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 7.0e-120
Identity = 219/563 (38.90%), Postives = 345/563 (61.28%), Query Frame = 0

Query: 4   KVVVSGMSLILVVGVALAVVAVINKGNKSEAMD-DLSPKMKSVQTICMGTDYKKECQNTL 63
           + V+  +S +L++ + +AV   ++      A D +++  +K+++ +C  TDYK+ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  ANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLLQF 123
              A + S  DP E VK A  ATM +++     + T M+E + +   KM++D CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQDGL 183
           AI  L  S+  +G    H   + +  L+ WLS+ +S +++C DG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRRLL 243
             A +LT N LA+V E+S  L     Q+++   N + L + E      P W+    RRLL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQE-----FPSWMDARARRLL 254

Query: 244 AGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKE 303
                  ++VKP++VVA+DGSGQ+KTIN ALN  PK     +V+++K GIY EY+ + + 
Sbjct: 255 ---NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 KVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAV 363
             ++   GDGP KT+++G KS +DG+TT  +A+ A++G  F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+R  +++S F+NC+ DGYQDTLY  ++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVI 483
           +P+ NQ   +TA+GRK  +E++G V+  C IV E    A + +  TYLGRPWKE++RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQF 543
           M + + D + P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E L+F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAAPFLLGKTWLQGTGGQYFMGL 566
           T A ++ G  W+ G G  Y +GL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Sed0010171 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 9.2e-120
Identity = 223/562 (39.68%), Postives = 345/562 (61.39%), Query Frame = 0

Query: 5   VVVSGMSLILVVGVALAVVAVINK-GNKSEAMDDLSPKMKSVQTICMGTDYKKECQNTLA 64
           + +S + LI +V VA+ V   +NK    S+   +++  +K+V+ +C  TDY+K C++TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  NIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLLQFA 124
            I +  ++ DP E VK A   TM ++T     + T+M E + ++  +M++D CK+L+ +A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQDGLD 184
           ++ L  S+  +G    H   + + +L+ WLS+ +S +E+C +G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRRLLA 244
            A +LT N LAI+ E+S  +     Q+++   N + L A     +G P W+    R+LL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLLA-----EGFPSWVDQRGRKLLQ 257

Query: 245 GARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEK 304
            A   S  VKP++VVA+DGSGQ+KTIN AL   PK     +V+++KAG+Y EY+ + K  
Sbjct: 258 AAAAYS-DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 317

Query: 305 VNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVA 364
            ++   GDGP KTI++G K+ +DG+TT  +A+ A++G  F+ K++GF+NTAG   HQAVA
Sbjct: 318 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 377

Query: 365 LRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRR 424
           +R QS++S FFNCR DGYQDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  ++VR+
Sbjct: 378 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 437

Query: 425 PMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIM 484
           P+ NQ   +TA+GRK  +E++G V   C I  E    A +     YLGRPWKE++RT+IM
Sbjct: 438 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 497

Query: 485 ESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFT 544
            + + D +QP G+ PW G F L T  Y E  N GPG+    RV W G++ +   + L+FT
Sbjct: 498 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 557

Query: 545 AAPFLLGKTWLQGTGGQYFMGL 566
            A ++ G  W+ G G  Y  GL
Sbjct: 558 PAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Sed0010171 vs. ExPASy TrEMBL
Match: A0A6J1JP54 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.7e-255
Identity = 442/568 (77.82%), Postives = 505/568 (88.91%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGN-KSEAMDDLSPKMKSVQTICMGTDYKKECQ 60
           MVGKVVVSG+S ILVVGVALAVVAV+NK N  +  M  LSPKMK+V +IC GT+Y KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           +TL+  A N++SDDP++Y+KAAI+AT++E+TKGLNLTDTL VEA +N++I+MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQ AI+SL AS STVGDP++HTDADR+ D+KTWLS+V+SFQ+SC DGL E D +L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
             LD AGKLTSNALAI+DEV  +LE +G+QLKV+P+ R+LLG TEVG DG P WL G DR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA ARGG+ +VKPN VVAKDGSGQFKTI AAL AYPK LRGRYVIYVKAG+YNEYII+
Sbjct: 241 KLLA-ARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK   NIFMYGDGPR TIVTGRKSNRDGVTTQ+SASF+ IG GFLCKSMGF NTAGPEGH
Sbjct: 301 TKNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS++SAFFNCRMDG+QDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQ+NTVTANGR   +EN+GLVIHNCRIVPEQKLFA+R KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQKNTVTANGRADNRENTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGDLIQPAGY+PWAG+FAL TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA
Sbjct: 481 TVIMESTLGDLIQPAGYMPWAGNFALDTCLYLEYGNRGPGAITNRRVKWKGVRVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G TWLQGTGG +F+GL+N
Sbjct: 541 MQFTAEPFLQGSTWLQGTGGPFFVGLKN 567

BLAST of Sed0010171 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 433/567 (76.37%), Postives = 500/567 (88.18%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           MVGKVV+SG+SLILVVGVALAVVA++NK + S+   +LSPKMK+V +IC   DY+ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLL 120
           TL ++ +N+S +DP+E++KAAI +T++E+ KG NLTD LMVEA NNA+IKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQD 180
           Q AI+ L ASYSTVGDP++HT+ DRI D+K WL++V+S+Q+SC DGL EFD +LKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRR 240
           GLDVAGKLTSNALAIV  VS++L+ +G+QLKV+P+ R+LLG TEV  D  P WL+G DR+
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIIT 300
           LLA  RGG  +VKPNVVVAKDGSGQ+KTI AAL AYPK L+GRYVIYVKAG+YNE II+T
Sbjct: 241 LLAAKRGG-VRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQ 360
           K+  NIFMYGDGPRKTIVTGRKSNRDG+TTQN+ASFA IG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QS++SAFFNCRMDGYQDTLYVQ +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHART 480
           VRRPMDNQQNTVTANGR  ++E SGLVIHNCRIVPEQKLFAER KIPT+LGRPWKE+ART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGD IQPAGY+PW+G+FAL+TC Y EY N+GPGANTNRRVRWKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           QFTA PFLLGK WL GTGG Y +GL+N
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Sed0010171 vs. ExPASy TrEMBL
Match: A0A6J1GYZ3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 3.0e-254
Identity = 440/568 (77.46%), Postives = 502/568 (88.38%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEA-MDDLSPKMKSVQTICMGTDYKKECQ 60
           M+GKVVVSG+S ILVVGVALAVVAV+NK N + A M  LSPKMK+V  IC GTDY KECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           +TL+  A N++SDDP++Y+KAAI+AT++E+TKGLNLTDTL VEA +N +I+MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQ AI+SL AS STVGDP++HTDADR+ D+KTWLS+V+SFQ+SC DGL E D +L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
             LD AGKLTSNALAI+DEV  +LE +G+QLKV+P+ R+LLG TEVG DG P WL G DR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA ARGG+ +VKPN VVAKDGSGQFKTI AAL AYPK L GRYVIYVKAG+Y+EYII+
Sbjct: 241 KLLA-ARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK   NIFMYGDGPR TIVTGRKSNRDGVTTQ+SASF+ IG GFLCKSMGF NTAGPEGH
Sbjct: 301 TKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS+KSAFFNCRMDG+QDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI
Sbjct: 361 QAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFA+R KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGDLIQP GY+PWAG+FAL TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA
Sbjct: 481 TVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G TWLQGTGG +F+GL+N
Sbjct: 541 MQFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Sed0010171 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.6e-250
Identity = 429/568 (75.53%), Postives = 500/568 (88.03%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSE-AMDDLSPKMKSVQTICMGTDYKKECQ 60
           MVGKV+VS +SLILVVG+ALAVVA INK N S    +++SPKMK++  IC  TDY++EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           NTL ++AHN SS+DP++Y KAAILAT+ E+TKG NL+D+L+VEA NNASIKMSV+DCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQFAI+ LQASYSTVG+ ++HTDADRI D+K WLS+VVS+Q+SC DGLGEFD +LKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
           DGLDVAGKLTSNALAIV  VS +L++ G+QLKV+P+ R+LLG TEV  DG P WL+G DR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA  +GG  +  PN VVAKDGSGQ+KTI AAL AYPK LRGRYVIYVKAGIY+EYI +
Sbjct: 241 KLLASKQGG-VRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK+  N+FMYGDGPRKTIVTGRKSNRDG TTQN+ASFA IG GFLCKSMGF NTAGP+GH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS++SAFFNCRMDG+QDTLYVQ +RQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQQNTVTA GR  ++E +GLVIHNCRIVPEQKLFAER KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGD IQPAGY+PW+G+FAL TC YLEY N+GPGANTNRRVRWKGV+VIGRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           LQ+TA  FLLG+TWL  TGG Y++GL+N
Sbjct: 541 LQYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Sed0010171 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.8e-250
Identity = 428/568 (75.35%), Postives = 495/568 (87.15%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSE-AMDDLSPKMKSVQTICMGTDYKKECQ 60
           MV KV++ G+SLILVVGVALAVVA+++    S+ A +DLSPKMK+V TIC   +Y++ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDL 120
           NTL+N AHNASSDDP+EYV+AAIL+T++EV KG NLTD  M+EA NN SIKM V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQ 180
           LQFAI+ LQASYSTVG+P++HT+ADR+ D+K WL+SV+S+Q+SC DGL EFD +LKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDR 240
           DGL  AGKLTSNALAIVD VS++L S G+QLK +P+ R+LLG TEVG+DG P WL+G DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 RLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIII 300
           +LLA +RGG A+VKPN VVAKDGSGQFKTI+AAL AYPK LRGRYVIYVKAGIYNEY+ I
Sbjct: 241 KLLA-SRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 TKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGH 360
           TK+  NIFMYGDGP+KTIVTG KSNR G TTQ++A+F  IG GFLCKSMGFQNTAGPEGH
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360

Query: 361 QAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420
           QAVALR QS++SAFFNCRMDGYQDTLYVQ  RQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHAR 480
           IVRRPMDNQQNTVTANGR   +E SGLVIHNCRIVPEQKLFAER KIPT+LGRPWK++AR
Sbjct: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEA 540
           TVIMESTLGD IQP GY+PW+G+FAL TC YLEY N+GPGA TNRRVRWKGV+VIGRNEA
Sbjct: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540

Query: 541 LQFTAAPFLLGKTWLQGTGGQYFMGLRN 568
           +QFTA PFL G+ WL  TGG Y +GL++
Sbjct: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Sed0010171 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 452.6 bits (1163), Expect = 4.7e-127
Identity = 267/603 (44.28%), Postives = 360/603 (59.70%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA INK   +    +LSP+MK+VQ IC  T  K  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA----NLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNAS--IKMSVDDCKD 120
           TL  +     S+DP + +KA +LAT DE+TK  N T    V   ++ S   K  +D CK 
Sbjct: 61  TLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKM 180
           +  +A+E L      +G+ ++     +ID LK WL  V ++Q  C D + E D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVDEVSELLESIGMQL--------------------------KV 240
            +G+  +  LT+NA+ I   V   +  I  ++                            
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 KPANRKLLGATEVGRDGIPIWLSGGDRRLLA-------GARGGSAQVKPNVVVAKDGSGQ 300
            PA R L    ++   GIP W+SG DR+L+A       G RGG A+V+ N VVAKDGSGQ
Sbjct: 241 GPARRLL---EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVTGRKS-- 360
           FKT+  A++A P+N RGR +IY+KAG+Y E +II K+K NIFM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCRMDGYQD 420
              G TT  SA+  V   GF+ K MGF+NTAGP GHQA A+R   +++  FNCR DGYQD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKY-RQE 480
           TLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G +     
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 NSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGYLPWAGS 540
             G+V+ NCRIVP++KL  ERL + TYLGRPWK+ + TVIM + +GDLI+P G+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQGTGGQYF 566
               +C+Y+EY N+GPGA  NRRV W  V      E   FTAA +L    W+Q       
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVT 588

BLAST of Sed0010171 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-124
Identity = 243/527 (46.11%), Postives = 330/527 (62.62%), Query Frame = 0

Query: 43  KSVQTICMGTDYKKECQNTLAN-IAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMV 102
           K +QT+C  T YK  CQNTL N    +    DPR  +K+AI+A  D++ +      +L  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 103 EAKNNASIKMSVDDCKDLLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQE 162
           E K++   K ++  CK L+  A E L  S   + D  V+  A  + DL +WLS+V+S+QE
Sbjct: 153 ENKDD---KDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 163 SCKDGLGEFDVELKQKMQDGLDVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLG 222
           +C DG  E   +LK +++   + +  LTSN+LA++  +   L S+    KVK   R LL 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KVK--TRLLLE 272

Query: 223 ATEVGR--DGIPIWLSGGDRRLLAGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKN 282
           A    +  D I  WLS  +RR+L         +KPN  VAKDGSG F TINAAL A P  
Sbjct: 273 ARSSAKETDHITSWLSNKERRMLKAV--DVKALKPNATVAKDGSGNFTTINAALKAMPAK 332

Query: 283 LRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVI 342
            +GRY IY+K GIY+E +II K+K N+ M GDG +KTIVTG KS+   + T  +A+F   
Sbjct: 333 YQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQ 392

Query: 343 GAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVI 402
           G GF+ +SMGF+NTAGPEGHQAVA+R QS++S F NCR +GYQDTLY   +RQ+YR+CVI
Sbjct: 393 GEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVI 452

Query: 403 SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKL 462
            GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR  + + +G VIHNC + P + L
Sbjct: 453 IGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDL 512

Query: 463 FAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGYLPWAGS-FALSTCQYLEYANKGP 522
              + +  +YLGRPWK H+RTV+MEST+ D+I P G+L W  + FA+ T  Y EY N GP
Sbjct: 513 KPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGP 572

Query: 523 GANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQGTGGQYFMGL 566
              T  RV+W G RV+ + EA++FT  PFL G+ W+Q  G    +GL
Sbjct: 573 SGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Sed0010171 vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.1 bits (1115), Expect = 1.7e-121
Identity = 257/609 (42.20%), Postives = 359/609 (58.95%), Query Frame = 0

Query: 1   MVGKVVVSGMSLILVVGVALAVVAVINKGNKSEAMDDLSPKMKSVQTICMGTDYKKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA INK   +    +LSP+MK+V+ IC  T  K  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA----NLSPQMKAVRGICEATSDKASCVK 60

Query: 61  TLANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNAS--IKMSVDDCKD 120
           TL  +     SDDP + +KA +LAT D +T+  N T        +  S   K  +D CK 
Sbjct: 61  TLEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCKK 120

Query: 121 LLQFAIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKM 180
           +  +A+E L      +G+ +++    +ID LK WL+ V ++Q  C D + E D  L++ +
Sbjct: 121 VFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKTI 180

Query: 181 QDGLDVAGKLTSNALAIVDEVSELLESIGMQLK----------------VKPAN------ 240
            +G+  +  LTSNA+ I   V   +  + ++++                  P N      
Sbjct: 181 GEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPPV 240

Query: 241 -------------RKLLGATEVGRDGIPIWLSGGDRRLLA----GARGGSAQVKPNVVVA 300
                        R+LL   ++   GIP W+SG DR+L+     G+  G A+++   VVA
Sbjct: 241 ADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVVA 300

Query: 301 KDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEKVNIFMYGDGPRKTIVT 360
           KDGSGQFKT+  A+NA P+   GR +I++KAGIY E +II K+K NIFM+GDG RKT+++
Sbjct: 301 KDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVIS 360

Query: 361 GRKSNR--DGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVALRSQSEKSAFFNCR 420
             +S +   G TT  S +  V   GF+ K +GF+NTAGP GHQAVA+R   +++  FNCR
Sbjct: 361 YNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNCR 420

Query: 421 MDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANG- 480
            DGYQDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA+G 
Sbjct: 421 FDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTADGN 480

Query: 481 RKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIMESTLGDLIQPAGY 540
            K      G+V+ NCRIVP++KL AERL + +YLGRPWK+ + TVI+ S +GD+I+P G+
Sbjct: 481 EKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRPEGW 540

Query: 541 LPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAAPFLLGKTWLQG 566
             W G     +C+Y+EY N+GPGA TNRRV W  +      E   FT A +L    W+Q 
Sbjct: 541 KIWDGESFHKSCRYVEYNNRGPGAITNRRVNWVKI-ARSAAEVNDFTVANWLGPINWIQE 595

BLAST of Sed0010171 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 432.6 bits (1111), Expect = 5.0e-121
Identity = 219/563 (38.90%), Postives = 345/563 (61.28%), Query Frame = 0

Query: 4   KVVVSGMSLILVVGVALAVVAVINKGNKSEAMD-DLSPKMKSVQTICMGTDYKKECQNTL 63
           + V+  +S +L++ + +AV   ++      A D +++  +K+++ +C  TDYK+ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  ANIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLLQF 123
              A + S  DP E VK A  ATM +++     + T M+E + +   KM++D CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQDGL 183
           AI  L  S+  +G    H   + +  L+ WLS+ +S +++C DG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRRLL 243
             A +LT N LA+V E+S  L     Q+++   N + L + E      P W+    RRLL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQE-----FPSWMDARARRLL 254

Query: 244 AGARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKE 303
                  ++VKP++VVA+DGSGQ+KTIN ALN  PK     +V+++K GIY EY+ + + 
Sbjct: 255 ---NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 KVNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAV 363
             ++   GDGP KT+++G KS +DG+TT  +A+ A++G  F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+R  +++S F+NC+ DGYQDTLY  ++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVI 483
           +P+ NQ   +TA+GRK  +E++G V+  C IV E    A + +  TYLGRPWKE++RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQF 543
           M + + D + P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E L+F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAAPFLLGKTWLQGTGGQYFMGL 566
           T A ++ G  W+ G G  Y +GL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Sed0010171 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 432.2 bits (1110), Expect = 6.5e-121
Identity = 223/562 (39.68%), Postives = 345/562 (61.39%), Query Frame = 0

Query: 5   VVVSGMSLILVVGVALAVVAVINK-GNKSEAMDDLSPKMKSVQTICMGTDYKKECQNTLA 64
           + +S + LI +V VA+ V   +NK    S+   +++  +K+V+ +C  TDY+K C++TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  NIAHNASSDDPREYVKAAILATMDEVTKGLNLTDTLMVEAKNNASIKMSVDDCKDLLQFA 124
            I +  ++ DP E VK A   TM ++T     + T+M E + ++  +M++D CK+L+ +A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IESLQASYSTVGDPNVHTDADRIDDLKTWLSSVVSFQESCKDGLGEFDVELKQKMQDGLD 184
           ++ L  S+  +G    H   + + +L+ WLS+ +S +E+C +G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVDEVSELLESIGMQLKVKPANRKLLGATEVGRDGIPIWLSGGDRRLLA 244
            A +LT N LAI+ E+S  +     Q+++   N + L A     +G P W+    R+LL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLLA-----EGFPSWVDQRGRKLLQ 257

Query: 245 GARGGSAQVKPNVVVAKDGSGQFKTINAALNAYPKNLRGRYVIYVKAGIYNEYIIITKEK 304
            A   S  VKP++VVA+DGSGQ+KTIN AL   PK     +V+++KAG+Y EY+ + K  
Sbjct: 258 AAAAYS-DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 317

Query: 305 VNIFMYGDGPRKTIVTGRKSNRDGVTTQNSASFAVIGAGFLCKSMGFQNTAGPEGHQAVA 364
            ++   GDGP KTI++G K+ +DG+TT  +A+ A++G  F+ K++GF+NTAG   HQAVA
Sbjct: 318 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 377

Query: 365 LRSQSEKSAFFNCRMDGYQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRR 424
           +R QS++S FFNCR DGYQDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  ++VR+
Sbjct: 378 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 437

Query: 425 PMDNQQNTVTANGRKYRQENSGLVIHNCRIVPEQKLFAERLKIPTYLGRPWKEHARTVIM 484
           P+ NQ   +TA+GRK  +E++G V   C I  E    A +     YLGRPWKE++RT+IM
Sbjct: 438 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 497

Query: 485 ESTLGDLIQPAGYLPWAGSFALSTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFT 544
            + + D +QP G+ PW G F L T  Y E  N GPG+    RV W G++ +   + L+FT
Sbjct: 498 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 557

Query: 545 AAPFLLGKTWLQGTGGQYFMGL 566
            A ++ G  W+ G G  Y  GL
Sbjct: 558 PAQYIQGDDWIPGKGVPYTTGL 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022990080.15.5e-25577.82pectinesterase-like [Cucurbita maxima][more]
XP_023527159.11.2e-25477.64pectinesterase-like [Cucurbita pepo subsp. pepo][more]
KGN49779.13.6e-25476.37hypothetical protein Csa_017783 [Cucumis sativus][more]
XP_022956875.16.1e-25477.46pectinesterase-like [Cucurbita moschata][more]
XP_038875879.11.5e-25275.84LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O807226.6e-12644.28Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q9SMY61.8e-12346.11Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q5MFV82.4e-12042.20Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2[more]
Q3E8Z87.0e-12038.90Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX869.2e-12039.68Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1JP542.7e-25577.82Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1[more]
A0A0A0KLN21.7e-25476.37Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A6J1GYZ33.0e-25477.46Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1[more]
A0A5A7U4V72.6e-25075.53Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A5A7U9J85.8e-25075.35Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
Match NameE-valueIdentityDescription
AT2G47030.14.7e-12744.28Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.3e-12446.11Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47040.11.7e-12142.20Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.15.0e-12138.90Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.16.5e-12139.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..195
e-value: 1.4E-31
score: 121.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..195
e-value: 1.3E-23
score: 84.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 22..198
e-value: 5.3E-17
score: 59.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 41..199
e-value: 4.8E-29
score: 103.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..198
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 255..550
e-value: 1.4E-128
score: 427.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 249..565
e-value: 5.1E-110
score: 369.8
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 5..564
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 5..564
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 4.7741E-47
score: 159.911
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 399..408
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 251..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0010171.1Sed0010171.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity