Homology
BLAST of Sed0009745 vs. NCBI nr
Match:
XP_023524039.1 (probable RNA-dependent RNA polymerase 1 isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 879/1115 (78.83%), Postives = 988/1115 (88.61%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
T G+TIE++GF R+ +A EVK ++ENHTG+GT+S V IS+PKD+K R T+A V F SK+A
Sbjct: 3 TKGKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISEPKDEKARLTYARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGVERMENVKGYFGNLVWEDKMRVIWK 123
AEYVV+KAEAEERLWF YLKAR V R VERME V+ G++VW+++MRVIW+
Sbjct: 63 AEYVVEKAEAEERLWFGRWYLKARGVERGREREMRVERMEKVRVQLGSVVWKEEMRVIWR 122
Query: 124 GENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSS 183
GENW +E+G RKL F+LS+G +YKMEL FENILG Q CP + SS LFLI++ G+
Sbjct: 123 GENWSVEYGNGMRKLWFYLSYGVIDYKMELRFENILGVQLRCPHNSSSNLFLIQL-QGAP 182
Query: 184 KIFKKTPLSSSSP-NSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFFQTLV 243
+IFKK P SSSS SK+STGF WIRDVDFTPSS IGQS ALC E+ PGD LPSFFQTLV
Sbjct: 183 RIFKKCPSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLPSFFQTLV 242
Query: 244 GFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLPGPALDDE 303
G+K+I +L GS+ SS S+LVPIV Q DIP+KILFKIN+LVQHGYLPG ALDD+
Sbjct: 243 GYKEIYGPFKLSTGSAFSSNSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSALDDK 302
Query: 304 FFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVSVDDG 363
FF+LVDP+RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK ++S+DD
Sbjct: 303 FFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANISLDDS 362
Query: 364 LVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDLAPR- 423
LVY+HRVQITP++VYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI SSDL+PR
Sbjct: 363 LVYVHRVQITPARVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDLSPRT 422
Query: 424 SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSASDIRD 483
S +K ERT VYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFA REGL+A+DIR+
Sbjct: 423 STEKIERTSVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFAPREGLNAADIRE 482
Query: 484 WMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSDGIGKI 543
WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPDVEV RK IKYCFSDGIGKI
Sbjct: 483 WMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCFSDGIGKI 542
Query: 544 SEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSLDTQLD 603
SEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKYMSLDTQLD
Sbjct: 543 SEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLDTQLD 602
Query: 604 VLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVLELMSP 663
VLSWSKY PC+LNRQVINLLSTLGI+D VFV +Q++A+ QLDSIL+D SKALEVLELMSP
Sbjct: 603 VLSWSKYQPCFLNRQVINLLSTLGIRDDVFVNQQKKAVDQLDSILRDPSKALEVLELMSP 662
Query: 664 GEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCLDETRT 723
GEMT+ILK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVPKGRTMMGCLDETR+
Sbjct: 663 GEMTSILKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETRS 722
Query: 724 LEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMVDCVVF 783
LEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLHHMVDCVVF
Sbjct: 723 LEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVDCVVF 782
Query: 784 PQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIEEVQE 843
PQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HDVTIEEVQE
Sbjct: 783 PQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTIEEVQE 842
Query: 844 YLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANLPGSL 903
Y ANYMVNDGLGGIANAHTVFADK P+KAMS+ECIELAKLFSIAVDFPKTGVPANLP SL
Sbjct: 843 YFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVPANLPRSL 902
Query: 904 RVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPDMEVDG 963
RV +YPDFMEKP++ SY+S GV GKLFRGVKDVSS+IN IETFTREVA+KCYDPDMEV G
Sbjct: 903 RVHQYPDFMEKPNKRSYISNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYDPDMEVMG 962
Query: 964 FQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQISIAMK 1023
F+NYL EAFD+K+ YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDMEQIS+AM+
Sbjct: 963 FENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQISLAMR 1022
Query: 1024 SLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDHFLSF 1083
SLRKEARGWFNEKGSK Y+++KD+++ +AKASAWY+VTYHPN+WG YNEGMQRDHFLSF
Sbjct: 1023 SLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQRDHFLSF 1082
Query: 1084 PWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
PWCVS+KL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 PWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLS 1113
BLAST of Sed0009745 vs. NCBI nr
Match:
XP_023524048.1 (probable RNA-dependent RNA polymerase 1 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1782.3 bits (4615), Expect = 0.0e+00
Identity = 879/1122 (78.34%), Postives = 989/1122 (88.15%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
T G+TIE++GF R+ +A EVK ++ENHTG+GT+S V IS+PKD+K R T+A V F SK+A
Sbjct: 3 TKGKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISEPKDEKARLTYARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGVERMENVKGYFGNLVWEDKMRVIWK 123
AEYVV+KAEAEERLWF YLKAR V R VERME V+ G++VW+++MRVIW+
Sbjct: 63 AEYVVEKAEAEERLWFGRWYLKARGVERGREREMRVERMEKVRVQLGSVVWKEEMRVIWR 122
Query: 124 GENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSS 183
GENW +E+G RKL F+LS+G +YKMEL FENILG Q CP + SS LFLI++ G+
Sbjct: 123 GENWSVEYGNGMRKLWFYLSYGVIDYKMELRFENILGVQLRCPHNSSSNLFLIQL-QGAP 182
Query: 184 KIFKKTPLSSSSPN--------SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLP 243
+IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS ALC E+ PGD LP
Sbjct: 183 RIFKKCPSSSSSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQLP 242
Query: 244 SFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLP 303
SFFQTLVG+K+I +L GS+ SS S+LVPIV Q DIP+KILFKIN+LVQHGYLP
Sbjct: 243 SFFQTLVGYKEIYGPFKLSTGSAFSSNSNLVPIVTPPQPFDIPYKILFKINALVQHGYLP 302
Query: 304 GPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKP 363
G ALDD+FF+LVDP+RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK
Sbjct: 303 GSALDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKA 362
Query: 364 SVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRS 423
++S+DD LVY+HRVQITP++VYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI S
Sbjct: 363 NISLDDSLVYVHRVQITPARVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHS 422
Query: 424 SDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGL 483
SDL+PR S +K ERT VYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFA REGL
Sbjct: 423 SDLSPRTSTEKIERTSVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFAPREGL 482
Query: 484 SASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCF 543
+A+DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPDVEV RK IKYCF
Sbjct: 483 NAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYCF 542
Query: 544 SDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYM 603
SDGIGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKYM
Sbjct: 543 SDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYM 602
Query: 604 SLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALE 663
SLDTQLDVLSWSKY PC+LNRQVINLLSTLGI+D VFV +Q++A+ QLDSIL+D SKALE
Sbjct: 603 SLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVNQQKKAVDQLDSILRDPSKALE 662
Query: 664 VLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMG 723
VLELMSPGEMT+ILK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVPKGRTMMG
Sbjct: 663 VLELMSPGEMTSILKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMG 722
Query: 724 CLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHH 783
CLDETR+LEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLHH
Sbjct: 723 CLDETRSLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHH 782
Query: 784 MVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDV 843
MVDCVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HDV
Sbjct: 783 MVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDV 842
Query: 844 TIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVP 903
TIEEVQEY ANYMVNDGLGGIANAHTVFADK P+KAMS+ECIELAKLFSIAVDFPKTGVP
Sbjct: 843 TIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGVP 902
Query: 904 ANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYD 963
ANLP SLRV +YPDFMEKP++ SY+S GV GKLFRGVKDVSS+IN IETFTREVA+KCYD
Sbjct: 903 ANLPRSLRVHQYPDFMEKPNKRSYISNGVIGKLFRGVKDVSSDINNIETFTREVAAKCYD 962
Query: 964 PDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDME 1023
PDMEV GF+NYL EAFD+K+ YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDME
Sbjct: 963 PDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDME 1022
Query: 1024 QISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQ 1083
QIS+AM+SLRKEARGWFNEKGSK Y+++KD+++ +AKASAWY+VTYHPN+WG YNEGMQ
Sbjct: 1023 QISLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGMQ 1082
Query: 1084 RDHFLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
RDHFLSFPWCVS+KL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 RDHFLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLS 1120
BLAST of Sed0009745 vs. NCBI nr
Match:
KAG7032582.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1778.1 bits (4604), Expect = 0.0e+00
Identity = 881/1125 (78.31%), Postives = 991/1125 (88.09%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
T G+TIE++GF R+ +A EVK ++ENHTG+GT+S V ISKPKDKK+R TFA V F SK+A
Sbjct: 3 TKGKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKDKKSRLTFARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRR-----NGVERMENVKGYFGNLVWEDKM 123
AEYVV+KAEAEERLWF YLKAR V R +ER+E V+ G+++ +D+M
Sbjct: 63 AEYVVEKAEAEERLWFGRWYLKARGVEREREREREMRVERLERLEKVRVQLGSVISKDEM 122
Query: 124 RVIWKGENWGMEFGTEDRKLSFHLSHGF--DEYKMELPFENILGYQFYCPLDRSSKLFLI 183
RVIW+GE+W +E+G RKL F+LS+G D+YKMEL FENILG Q CP + SS LFLI
Sbjct: 123 RVIWRGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLI 182
Query: 184 KVFAGSSKIFKKTPLSSSSPN----SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGD 243
++ G+ +IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS ALC E+ PGD
Sbjct: 183 QL-QGAPRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGD 242
Query: 244 HLPSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHG 303
LPSFFQTLVG+K+I +L GS+ SS S+LVPIV Q IP+KILFKIN+LVQHG
Sbjct: 243 QLPSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHG 302
Query: 304 YLPGPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLP 363
YLPG ALDD+FF+LVDP RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLP
Sbjct: 303 YLPGSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLP 362
Query: 364 WKPSVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDK 423
WK ++S+DD LVY+HRVQITP+KVYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDK
Sbjct: 363 WKANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDK 422
Query: 424 IRSSDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASR 483
I SSDL+PR S +K ERTRVYDRI SVLRNGI+IG KKFEFLAFSASQLR+NSFWMFASR
Sbjct: 423 IHSSDLSPRTSTEKIERTRVYDRILSVLRNGILIGGKKFEFLAFSASQLRENSFWMFASR 482
Query: 484 EGLSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIK 543
EGL+A+DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPDVEV RK IK
Sbjct: 483 EGLNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIK 542
Query: 544 YCFSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSML 603
YCFSDGIGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SML
Sbjct: 543 YCFSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSML 602
Query: 604 KYMSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSK 663
KYMSLDTQLDVLSWSKY PC+LNRQVINLLSTLGIQD VFVK+Q++A+ QLDSIL+D SK
Sbjct: 603 KYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSK 662
Query: 664 ALEVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRT 723
ALEVLELMSPGEMT++LK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVP+GRT
Sbjct: 663 ALEVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRT 722
Query: 724 MMGCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKT 783
MMGCLDETRTLEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKT
Sbjct: 723 MMGCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKT 782
Query: 784 LHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLN 843
LHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+
Sbjct: 783 LHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLD 842
Query: 844 HDVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKT 903
HDVTIEEVQEY ANYMVNDGLGGIANAHTVFADK P+KAMS+ECIELAKLFSIAVDFPKT
Sbjct: 843 HDVTIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKT 902
Query: 904 GVPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASK 963
GVPANLP SLRV EYPDFMEKP++ +Y+S GV GKLFRGVKDVSS+IN IETFTREVA+K
Sbjct: 903 GVPANLPRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAAK 962
Query: 964 CYDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRN 1023
CYDPDMEV GF+NYL EAFD+K+ YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRN
Sbjct: 963 CYDPDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRN 1022
Query: 1024 DMEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNE 1083
DMEQIS+AM+SLRKEARGWFNEKGSK Y+++KD+++ +AKASAWY+VTYHPN+WG YNE
Sbjct: 1023 DMEQISLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNE 1082
Query: 1084 GMQRDHFLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
GMQRDHFLSFPWCVS+KL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 GMQRDHFLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLS 1123
BLAST of Sed0009745 vs. NCBI nr
Match:
XP_022923210.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita moschata])
HSP 1 Score: 1775.4 bits (4597), Expect = 0.0e+00
Identity = 881/1123 (78.45%), Postives = 989/1123 (88.07%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
T G+TIE++GF R+ +A EVK ++ENHTG+GT+S V ISKPK +K+R T A V F SK+A
Sbjct: 3 TKGKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGV---ERMENVKGYFGNLVWEDKMRV 123
AEYVV+KAEAEERLWF YLKAR V R V ER+E V+ G+++W+D+MRV
Sbjct: 63 AEYVVEKAEAEERLWFGRWYLKARRVEREREREMRVERLERLEKVRVQLGSVIWKDEMRV 122
Query: 124 IWKGENWGMEFGTEDRKLSFHLSHGF--DEYKMELPFENILGYQFYCPLDRSSKLFLIKV 183
IW+GE+W +E+G RKL F+LS+G D+YKMEL FENILG Q CP + SS LFLI++
Sbjct: 123 IWRGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL 182
Query: 184 FAGSSKIFKKTPLSSSSPN----SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHL 243
G+ +IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS ALC E+ PGD L
Sbjct: 183 -QGAPRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQL 242
Query: 244 PSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYL 303
PSFFQTLVG+K+I +L GS+ SS S+LVPIV Q IP+KILFKIN+LVQHGYL
Sbjct: 243 PSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYL 302
Query: 304 PGPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWK 363
PG ALDD+FF+LVDP RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK
Sbjct: 303 PGSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWK 362
Query: 364 PSVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIR 423
++S+DD LVY+HRVQITP+KVYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI
Sbjct: 363 ANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIY 422
Query: 424 SSDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREG 483
SSDL+PR S +K ERTRVYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFASREG
Sbjct: 423 SSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREG 482
Query: 484 LSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYC 543
L+A+DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPDVEV RK IKYC
Sbjct: 483 LNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYC 542
Query: 544 FSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKY 603
FSDGIGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKY
Sbjct: 543 FSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKY 602
Query: 604 MSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKAL 663
MSLDTQLDVLSWSKY PC+LNRQVINLLSTLGIQD VFVK+Q++A+ QLDSIL+D SKAL
Sbjct: 603 MSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKAL 662
Query: 664 EVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMM 723
EVLELMSPGEMT++LK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVP+GRTMM
Sbjct: 663 EVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMM 722
Query: 724 GCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLH 783
GCLDETRTLEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLH
Sbjct: 723 GCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLH 782
Query: 784 HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHD 843
HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HD
Sbjct: 783 HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHD 842
Query: 844 VTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGV 903
VTIEEVQEY ANYMVNDGLGGIANAHTVFADK P+KAMS+ECIELAKLFSIAVDFPKTGV
Sbjct: 843 VTIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGV 902
Query: 904 PANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCY 963
PANLP SLRV EYPDFMEKP++ +Y S GV GKLFRGVKDVSS+IN IETFTREVA+KCY
Sbjct: 903 PANLPRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAAKCY 962
Query: 964 DPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDM 1023
DPDMEV GF+NYL EAFD+K+ YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDM
Sbjct: 963 DPDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDM 1022
Query: 1024 EQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGM 1083
EQIS+AM+SLRKEARGWFNEKGSK Y+++KD+++ +AKASAWY+VTYHPN+WG YNEGM
Sbjct: 1023 EQISLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGM 1082
Query: 1084 QRDHFLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
QRDHFLSFPWCVS+KL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 QRDHFLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLS 1121
BLAST of Sed0009745 vs. NCBI nr
Match:
XP_022990904.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita maxima])
HSP 1 Score: 1775.0 bits (4596), Expect = 0.0e+00
Identity = 881/1119 (78.73%), Postives = 986/1119 (88.11%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
TNG+TIE++GFRR+ +A EVK ++ENHTG+GT+S V ISKPKD K R TFA V F SK+A
Sbjct: 3 TNGKTIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGVERMENVKGYFGNLVWEDKMRVIWK 123
AEYVV+KAEAEE LWF YLKAR V MR VERME V+ G+++ +++MRVIW+
Sbjct: 63 AEYVVEKAEAEEGLWFGRWYLKARGVEREMR----VERMEKVRVQLGSVISKEEMRVIWR 122
Query: 124 GENWGMEFGTEDRKLSFHLSHGF--DEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAG 183
GE W +E+G RKL F+LS+G D+YKMEL FENILG Q CP + SS LFLI++ G
Sbjct: 123 GEEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL-QG 182
Query: 184 SSKIFKKTPLSSSSPN---SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFF 243
+ +IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS +LCLE+ PGD LPSFF
Sbjct: 183 APRIFKKCPSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQLPSFF 242
Query: 244 QTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLPGPA 303
QTLVG+K+I +L GS+ SS S+LVPIV Q DIP+KILFKIN+LVQHGYLPG A
Sbjct: 243 QTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSA 302
Query: 304 LDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVS 363
LDD+FF+LVDP+RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK ++S
Sbjct: 303 LDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANIS 362
Query: 364 VDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDL 423
+DD LVY+HRVQITP+KVYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI SSDL
Sbjct: 363 LDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDL 422
Query: 424 APR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSAS 483
+PR S +K ERTRVYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFASREGL+A+
Sbjct: 423 SPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLNAA 482
Query: 484 DIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSDG 543
DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPD EV RK IKYCFSDG
Sbjct: 483 DIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKYCFSDG 542
Query: 544 IGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSLD 603
IGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKYMSLD
Sbjct: 543 IGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLD 602
Query: 604 TQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVLE 663
TQLDVLSWSKY PC+LNRQVINLLSTLGI+D VFVK+Q++A+ QLDSIL+D SKALEVLE
Sbjct: 603 TQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKALEVLE 662
Query: 664 LMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCLD 723
LMSPGEMT++LK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVPKGRTMMGCLD
Sbjct: 663 LMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLD 722
Query: 724 ETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMVD 783
ETRTLEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLHHMVD
Sbjct: 723 ETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVD 782
Query: 784 CVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIE 843
CVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HDVT+E
Sbjct: 783 CVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTLE 842
Query: 844 EVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANL 903
EVQEY ANYMVNDGLGGIANAHTVFAD P+KAMS+ECIELAKLFSIAVDFPKTGVPANL
Sbjct: 843 EVQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTGVPANL 902
Query: 904 PGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPDM 963
P SLRV EYPDFMEKP++ +Y+S GV GKLFRGVKDVSS+IN IETFTREVA KCYDPDM
Sbjct: 903 PRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKCYDPDM 962
Query: 964 EVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQIS 1023
EV GF+NYL EAFD+K YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDMEQIS
Sbjct: 963 EVMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQIS 1022
Query: 1024 IAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDH 1083
+AM+SLRKEARGWFNEK SK Y+++K ++ +AKASAWY+VTYHPN+WG YNEGMQRDH
Sbjct: 1023 LAMRSLRKEARGWFNEKASKSIYNNNKGEDNEYAKASAWYHVTYHPNFWGRYNEGMQRDH 1082
Query: 1084 FLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
FLSFPWCVSDKL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 FLSFPWCVSDKLIQIKREKM---ESSPVSSLIHKFGKLS 1113
BLAST of Sed0009745 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 610/1102 (55.35%), Postives = 782/1102 (70.96%), Query Frame = 0
Query: 6 GRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLAAE 65
G+TI+++GF +A+EVK +LE TG GT+ + + +PK R +A V+FTS+
Sbjct: 2 GKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTR 61
Query: 66 YVVKKAEAEERLWFRDSYLKARAVAAPM--RRRNGVERMENVKGYFGNLVWEDKMRVIWK 125
++ A ERL++ SYLKA V + + R + + +K +FG V K +W
Sbjct: 62 LII--TAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWS 121
Query: 126 GENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSS 185
++ + FG RKL F S +Y++EL +ENI + P RSSK +I+V G+
Sbjct: 122 AQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI-GAP 181
Query: 186 KIFKKTP-----LSSSSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFF 245
KIF+K L + WIR DFT SS IGQS+A CLEL ++P F
Sbjct: 182 KIFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFR 241
Query: 246 QTLVGF-KQICARLELQPGSS-SSSPSDLVPIVQ---RLDIPFKILFKINSLVQHGYLPG 305
+ + + + ++ GSS SS+ + LVP+V +PF+ILFK+N+LVQ+ L G
Sbjct: 242 ENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSG 301
Query: 306 PALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPS 365
PALD +F++L++ ++ I++ LEKLF+L ECCYEP WL+++Y S +LP P+
Sbjct: 302 PALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPT 361
Query: 366 VSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSS 425
+S+DDGLVY++RVQ+TP++VYF GPE N+SNRV+R +S I++F+RVSFVDE+L+K+RS
Sbjct: 362 ISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSM 421
Query: 426 DLAPRSADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSA 485
DL+PRS+ + RT++YDRI+SVLR+GIVIG+KKFEFLAFS+SQLR+NS WMFA + ++A
Sbjct: 422 DLSPRSSTQ-RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 481
Query: 486 SDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSD 545
+ IR WMGDF +IRNVAKYAARLGQSF SSR+TL V EIE+IPDVE++ +Y FSD
Sbjct: 482 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSD 541
Query: 546 GIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSL 605
GIGKIS A KVA +CGL +PSAFQIRY G+KGVVA+DP S+KKLSLR SM K+ S
Sbjct: 542 GIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESE 601
Query: 606 DTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVL 665
+T+LDVL+WSKY PCY+NRQ+I LLSTLG+ D VF KKQ E + +LD+IL +A E L
Sbjct: 602 NTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEAL 661
Query: 666 ELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGC 725
LM+PGE TNILK ++L Y P EPFL+MML+ FRA KLL+L+ K+RIF+ GR+MMGC
Sbjct: 662 GLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGC 721
Query: 726 LDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHM 785
LDETRTLEYGQV Q S P + + F++ G VVVAKNPCLHPGDVRV AV+V L+HM
Sbjct: 722 LDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHM 781
Query: 786 VDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVT 845
VDCVVFPQKG RPHPNECSGSDLDGD+YFV WD EL RT +PM Y P PT L+HDVT
Sbjct: 782 VDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVT 841
Query: 846 IEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPA 905
IEEV+EY ANY+VND LG IANAHT FADK P KA S CIELAK FS AVDFPKTGV A
Sbjct: 842 IEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAA 901
Query: 906 NLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDP 965
+P L VKEYPDFMEKP +P+Y SK V GKLFR VK+ + + I++FT +VASK YD
Sbjct: 902 VIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDK 961
Query: 966 DMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQ 1025
DMEVDGF+ Y+ EAF K YDFKLGNLM YYGI+TE E++ G I+RM KSF KR D E
Sbjct: 962 DMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAES 1021
Query: 1026 ISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQR 1085
I A+++LRKE FN ++++NE AKASAWY+VTYH +YWGLYNEG+ R
Sbjct: 1022 IGRAVRALRKETLSLFNA--------SEEEENES-AKASAWYHVTYHSSYWGLYNEGLNR 1081
Query: 1086 DHFLSFPWCVSDKLLQIKREKM 1095
DHFLSF WCV DKL++IK+ +
Sbjct: 1082 DHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of Sed0009745 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 896.7 bits (2316), Expect = 2.6e-259
Identity = 451/734 (61.44%), Postives = 558/734 (76.02%), Query Frame = 0
Query: 371 SKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDLAPRSAD--KSERTRV 430
S VYF GPE N+SNRVVR FS DI++F+R+SFVDE+ +K+R++DL+PRSA + RT +
Sbjct: 7 STVYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTAL 66
Query: 431 YDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSASDIRDWMGDFSNIRN 490
Y R+ SVL +GI IG K FEFLAFS+SQLRDNS WMFASR+GL+ASDIR WMGDF NIRN
Sbjct: 67 YKRVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRN 126
Query: 491 VAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSDGIGKISEAAAEKVAE 550
VAKYAARLGQSF SS +TL V+K E+E I D ++ ++ FSDGIGKIS A A +VA
Sbjct: 127 VAKYAARLGQSFSSSTETLKVQKYEVEEISD---IKNGTQHVFSDGIGKISSAFANEVAM 186
Query: 551 ECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSLDTQLDVLSWSKYHPC 610
+C L PSAFQIRY G+KGVVA+DPTS KLSLR SMLK+ S + +DVL++SKY P
Sbjct: 187 KCNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPG 246
Query: 611 YLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVLELMSPGEMTNILKEM 670
+LNRQ+I LLSTLG++D VF +KQEEA+ QL+ ++ D A+E +ELM GE+TN +KE+
Sbjct: 247 FLNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKEL 306
Query: 671 LLL-YNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCLDETRTLEYGQVFAQ 730
LL Y P +EP+L+M+L+TFRA KLL+LK KSRI +PKGR MMGCLDETRTL+YGQVF +
Sbjct: 307 LLCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR 366
Query: 731 CSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMVDCVVFPQKGKRPHP 790
+ G F V GKVV+AKNPCLHPGD+R+ AVDV LHHM +CVVFPQ+G RPHP
Sbjct: 367 -ATSGVNDNDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVVFPQQGPRPHP 426
Query: 791 NECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIEEVQEYLANYMVND 850
NECSGSDLDGD+YFVSWDP L R V PM Y PAPT L+HDVTIEEV+EY NY+VN+
Sbjct: 427 NECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVEEYFTNYIVNE 486
Query: 851 GLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANLPGSLRVKEYPDFM 910
LG IANAH VFADK KA SS CIELAKLFSIAVDFPKTGVPA +P L VKEYPDFM
Sbjct: 487 SLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPELHVKEYPDFM 546
Query: 911 EKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPDMEVDGFQNYLGEAF 970
EK + +Y SKGV GKL+R +K + + I+ FTREVA + YD DM VDG+++Y+ EA
Sbjct: 547 EKLDKVTYESKGVIGKLYREIKKHTPH---IKHFTREVARRSYDTDMIVDGYEDYITEAM 606
Query: 971 DFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQISIAMKSLRKEARGW 1030
K YDFKLGNLM +YGI++E E++ G IL+M K+F K++D + I +A++SLRKEAR
Sbjct: 607 ALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAVRSLRKEARSR 666
Query: 1031 FNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDHFLSFPWCVSDKLL 1090
F+E D+ + AKASAWY+VTYHP +WG YNEG +R HF+SFPWC+ +KLL
Sbjct: 667 FSEMSLD---DNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFISFPWCIYEKLL 726
Query: 1091 QIKREKMCMINSSP 1102
+IK+ + + P
Sbjct: 727 RIKQRRKFVRKMQP 730
BLAST of Sed0009745 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 745.3 bits (1923), Expect = 9.7e-214
Identity = 441/1139 (38.72%), Postives = 667/1139 (58.56%), Query Frame = 0
Query: 2 TTTNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSK 61
TTTN T+++ + ADE+ +LE H G+ T+ + I +D FA V+FT+
Sbjct: 5 TTTNRSTVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT- 64
Query: 62 LAAEYVVKKAEAEERLWFRDSYLKAR-----AVAAPMRRRNGVERMENVKGYFGNLVWED 121
L + + ++ +L F+ L+ + P+ R ++ + G+ + E
Sbjct: 65 LEVKSRAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPES--DEK 124
Query: 122 KMRVIWKGENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKL--F 181
+ + K + TE R++ F + D YK+E+ FE+I+ C +S++ F
Sbjct: 125 RFCALEKWDGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAF 184
Query: 182 LIKVFAGSSKIFKKTPL-------SSSSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLE 241
L+K+ G K+FK+ + S K+ F WIR DF+ S SIG S+ CLE
Sbjct: 185 LLKLKYG-PKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLE 244
Query: 242 LQPGDHLPSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIVQR----LDIPFKILFKIN 301
+ G + F L +++ L G + +S + +VP++ L+ P++ILF++N
Sbjct: 245 VHNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLN 304
Query: 302 SLVQHGYLP-GPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSL 361
+LV + A D E +++ + + L+KL CY+P ++K + S+
Sbjct: 305 ALVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSV 364
Query: 362 YSSKQLPWKPSVS----VDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFI 421
K++ P+ + + ++ R +TPSK+Y GPE +N VV+ F++ + F+
Sbjct: 365 V--KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFM 424
Query: 422 RVSFVDEELDKIRSSDLAPRSAD----KSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSA 481
RV+FV+E+ K+ ++ L+ S + K RT +Y+R+ S+L GI +G K+FEFLAFSA
Sbjct: 425 RVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSA 484
Query: 482 SQLRDNSFWMFASREGLSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEI 541
SQLR NS WMFAS E + A DIR+WMG F IR+++K AAR+GQ F +SR+TL V ++
Sbjct: 485 SQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDV 544
Query: 542 EIIPDVEVVRKEIKYCFSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAID 601
E IPD+EV YCFSDGIGKIS A A++VA++CG L H PSAFQIRY G+KGV+A+D
Sbjct: 545 EQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCG-LSHVPSAFQIRYGGYKGVIAVD 604
Query: 602 PTSTKKLSLRDSMLKYMSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEE 661
+S +KLSLRDSMLK+ S + L+V W++ PC+LNR++I LLSTLGI+D +F Q
Sbjct: 605 RSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAV 664
Query: 662 AIYQLDSILKDSSKALEVLELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLL 721
+ L ++L+D AL VL+ +S N+L +MLL Y P EP+L+MMLR +L
Sbjct: 665 HLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLS 724
Query: 722 DLKNKSRIFVPKGRTMMGCLDETRTLEYGQVFAQCSVP------------GKASKSSFVV 781
+LK++ RI VPKGR ++GC+DE LEYGQV+ + ++ K + + VV
Sbjct: 725 ELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVV 784
Query: 782 KGKVVVAKNPCLHPGDVRVFDAVDVKTLHH--MVDCVVFPQKGKRPHPNECSGSDLDGDL 841
GKVVV KNPCLHPGD+RV DA+ +DC++FPQKG+RPHPNECSG DLDGD
Sbjct: 785 IGKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQ 844
Query: 842 YFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVF 901
+FVSWD ++ PM Y + ++HDVT+EE+ ++ +YM++D LG I+ AH V
Sbjct: 845 FFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVH 904
Query: 902 ADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKG 961
AD+ P+KA S +C+ELA L S AVDF KTG PA +P +L+ +E+PDF+E+ +P+Y+S+
Sbjct: 905 ADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISES 964
Query: 962 VFGKLFRGVKDVSSNINIIETFTREVASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGN 1021
VFGKL+R VK S++ + + YD +E GF++++ A + Y KL +
Sbjct: 965 VFGKLYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTS 1024
Query: 1022 LMGYYGIETEPELVGGNILRMGKSFDKRND------MEQISIAMKSLRKEARGWFNEKGS 1081
LM YYG E E++ G IL+ + + R++ ++I++++K L KEA GWF +
Sbjct: 1025 LMIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEK--- 1084
Query: 1082 KCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDHFLSFPWCVSDKLLQIKRE 1093
C +D+ + ASAWYYVTY+PN+ ++ FLSFPW V D LL IK E
Sbjct: 1085 SC-----EDEQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAE 1116
BLAST of Sed0009745 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 706.1 bits (1821), Expect = 6.5e-202
Identity = 404/999 (40.44%), Postives = 582/999 (58.26%), Query Frame = 0
Query: 136 RKLSFHLSHGFDEYKMELPFENI---LGYQFYCPLDRSSKLFLIKVFAGSSKIFKKTPLS 195
R++ +L H YK+E+ FE++ LG C LD + L +A T +S
Sbjct: 142 RRVDLYLEHDSQRYKLEVLFEDMKDCLG----CTLDGMGAILLQLNYAPRI----HTAIS 201
Query: 196 SSSPNS----------KKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFFQTLV 255
+ NS K+ F W+R +DFTP+ S G+ S L L+L + ++L
Sbjct: 202 GPAVNSRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDILKSL- 261
Query: 256 GFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLPGPALDDE 315
F L + + S++VP+V + +P+++LF++NSL+ G + ++ +
Sbjct: 262 PFSGNLGELTMNSMDGVGASSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAKHVNAD 321
Query: 316 FFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQ--LPWKPSVSVD 375
F+ + D EK+ L+ CY P ++++++ S+ S L + +
Sbjct: 322 LFKAL--QELPVDVSRRIFEKMHKLESTCYGPLQFIQQEAYSMKRSHNVLLSNEGEGEGE 381
Query: 376 DGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDLAP 435
L+ +RV ITPSK++ GPE ++N VV+ S F+RV+FVDE+ K+ S+ ++
Sbjct: 382 RKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDWSKLSSNAISA 441
Query: 436 RSA----DKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSA 495
R K +T +Y RI S+L+ G IG K FEFLAFSASQLR NS WMFAS L+A
Sbjct: 442 RIEQGFFSKPFKTGLYYRILSILKKGFSIGPKNFEFLAFSASQLRGNSVWMFASNASLNA 501
Query: 496 SDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSD 555
IR WMG F NIR+V+K AAR+GQ F SSR+T V + ++E+IPD+E+ KY FSD
Sbjct: 502 GGIRRWMGHFENIRSVSKCAARMGQLFSSSRQTFEVLRWDVEVIPDIEITTDGSKYIFSD 561
Query: 556 GIGKISEAAAEKVAEECGL-LCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMS 615
GIGKIS A++VA GL + PSAFQIRY G+KGV+AIDP S+ LSLR SM K+ S
Sbjct: 562 GIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDLSLRPSMKKFES 621
Query: 616 LDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEV 675
L++ SWSK PCY+NR++I+LLSTLGI+D +FV Q++ + + + +L + AL V
Sbjct: 622 ESRMLNITSWSKSQPCYVNREIISLLSTLGIRDEIFVAMQQDEMRETEEMLTNKEVALSV 681
Query: 676 LELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGC 735
L + E +K +L Y P EP+L+M+L+ + ++L D++ + +I VPKGR ++GC
Sbjct: 682 LGKLGGSETKTAVKMLLQGYEPSSEPYLSMILKAHQENRLTDIRTRCKIHVPKGRVLIGC 741
Query: 736 LDETRTLEYGQVFAQCSVPGKASKSS------------FVVKGKVVVAKNPCLHPGDVRV 795
LDET LEYGQV+ + + K K S V GKV + KNPCLHPGD+RV
Sbjct: 742 LDETGVLEYGQVYIRITKNSKEQKDSNQSYFYNDDGKTATVVGKVAITKNPCLHPGDIRV 801
Query: 796 FDAVDVKTLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYE 855
+A+ L MVDC+VFPQ+G+RPHPNECSG DLDGDLYF++WD +L + PM Y
Sbjct: 802 LEAIYDPDLVGMVDCLVFPQRGERPHPNECSGGDLDGDLYFITWDDKLIPEKVDTPMDYT 861
Query: 856 PAPTIQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFS 915
++H VT+EE+Q++ +YM+ND LG I+ AH + AD+SP KA S EC++LA L S
Sbjct: 862 ATRPRIMDHVVTLEEIQKHFVDYMINDSLGAISTAHLIHADRSPLKARSPECLQLATLHS 921
Query: 916 IAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFR---GVKDVSSNINI 975
+AVDF KTG PA +P +LR +EYPDFME+ +P Y+S GV GKL+R G + S +
Sbjct: 922 MAVDFAKTGAPAEMPRTLRPREYPDFMERWEKPMYISNGVLGKLYRSAMGHMEKSGDSGA 981
Query: 976 IETFTREVASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNI 1035
+ + + + S YDPD+EV G +L A ++ Y+ KL LM YY E E E++ GNI
Sbjct: 982 LSSSSAQ-PSPTYDPDLEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRAELEDEILTGNI 1041
Query: 1036 LRMGKSFDKRND------MEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKAS 1091
R + KR++ ++I A+ +L +EARGW + + + AS
Sbjct: 1042 -RNKMLYLKRDNKRYFEMKDRIVAAVDALHREARGWLL---------SSRKEEDASRMAS 1101
BLAST of Sed0009745 vs. ExPASy Swiss-Prot
Match:
Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)
HSP 1 Score: 571.2 bits (1471), Expect = 2.5e-161
Identity = 400/1081 (37.00%), Postives = 570/1081 (52.73%), Query Frame = 0
Query: 94 RRRNGVE-RMENVKGYFGNLVWEDKMRVIWKGENWGMEFGTE----DRKLSFHLSHGFD- 153
RRR V ++ + G LV D V W+ E G++F + K F S F
Sbjct: 123 RRRTTVPYKLAGITLEIGTLVSRDDFFVSWRAE--GVDFLVDPFDNTCKFCFRKSTAFSF 182
Query: 154 -----------EYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSSKIFKKT---PLSS 213
+YK+EL +I + Y L LI A S +++ +T +
Sbjct: 183 KDAVMHAVINCDYKLELLVRDIQTVRQYKTL---HGFVLILQLASSPRVWYRTADDDIYD 242
Query: 214 SSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQP------GDHLPSFFQTLVGFKQI 273
+ P PWIR DFT +IG+ + + + P L F V +++
Sbjct: 243 TVPGDLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERV 302
Query: 274 --CARLELQPGSSSSSPSDLVPIVQRLDIPFKILFKINSLVQHGYLPGPALDDEFFQLVD 333
R+ +P I + I F+I+F +NS++ G L + FF L+
Sbjct: 303 RWPPRIRNEPCFGEPVSDHFFCIHHKEGISFEIMFLVNSVLHRGVFNQFQLTERFFDLL- 362
Query: 334 PNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVSVDDGLVYIHR 393
D +L+ L K ++ +K LK + + +L S D + I R
Sbjct: 363 -RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRR 422
Query: 394 VQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDL----AP---- 453
+ ITP++ Y PE LSNRV+RR+ + F+RV+F+DE + I S+ L AP
Sbjct: 423 LVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKD 482
Query: 454 -RSADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSASDI 513
S+ S++T V+ R+ S+L +G + +K+ FLAFSA+QLRD S W FA SDI
Sbjct: 483 LTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDI 542
Query: 514 RDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEI-IPDVEVVRKEIKYCFSDGI 573
+ WMG F + +NVAK AAR+G F S+ T+ V E++ +PD+E Y FSDGI
Sbjct: 543 KTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVFSDGI 602
Query: 574 GKISEAAAEKVAEECGLLCH-TPSAFQIRYAGFKGVVAIDPTSTK--KLSLRDSMLKYMS 633
G I+ A++V E+ L H +P A+QIRYAGFKGVVA P+ + +L+LRDSM K+ S
Sbjct: 603 GTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFS 662
Query: 634 LDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEV 693
T L++ SW+++ P +LNRQ+I LLS LG+ D +F QE +Y+L+ IL D+ A EV
Sbjct: 663 KHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEV 722
Query: 694 LELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMG 753
L S E N ML + P EP L ML + R +L L+ KSRIFV GR +MG
Sbjct: 723 L-TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMG 782
Query: 754 CLDETRTLEYGQVFAQCSVPG------------KASKSSF-VVKGKVVVAKNPCLHPGDV 813
CLDE LE+GQ F Q S P K +K+ VVKG V +AKNPCLHPGDV
Sbjct: 783 CLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDV 842
Query: 814 RVFDAVDVKTLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPEL--TCIRTVKP 873
R+ +AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV+WD +L ++
Sbjct: 843 RILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPNRKSYPA 902
Query: 874 MSYEPAPTIQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELA 933
M Y+ A L V +++ ++ A + N+ LG I NAH V AD+S AM EC+ LA
Sbjct: 903 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 962
Query: 934 KLFSIAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDV-SSNI 993
+L + AVDFPKTG ++P L+ K YPDFM K +Y S + G+L+R VK+V +
Sbjct: 963 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1022
Query: 994 NIIETFTREVASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGG 1053
+ + ++ YD +E+ GF++ + EA+ K YD +L L+G Y ++ E E+V G
Sbjct: 1023 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1082
Query: 1054 NILRMGKSFDKRND--MEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGF-AKASA 1106
+I M K K+ E++ + SL+KE R F E +++ N + KASA
Sbjct: 1083 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYEKKASA 1142
BLAST of Sed0009745 vs. ExPASy TrEMBL
Match:
A0A6J1EB47 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 SV=1)
HSP 1 Score: 1775.4 bits (4597), Expect = 0.0e+00
Identity = 881/1123 (78.45%), Postives = 989/1123 (88.07%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
T G+TIE++GF R+ +A EVK ++ENHTG+GT+S V ISKPK +K+R T A V F SK+A
Sbjct: 3 TKGKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGV---ERMENVKGYFGNLVWEDKMRV 123
AEYVV+KAEAEERLWF YLKAR V R V ER+E V+ G+++W+D+MRV
Sbjct: 63 AEYVVEKAEAEERLWFGRWYLKARRVEREREREMRVERLERLEKVRVQLGSVIWKDEMRV 122
Query: 124 IWKGENWGMEFGTEDRKLSFHLSHGF--DEYKMELPFENILGYQFYCPLDRSSKLFLIKV 183
IW+GE+W +E+G RKL F+LS+G D+YKMEL FENILG Q CP + SS LFLI++
Sbjct: 123 IWRGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL 182
Query: 184 FAGSSKIFKKTPLSSSSPN----SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHL 243
G+ +IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS ALC E+ PGD L
Sbjct: 183 -QGAPRIFKKCPSSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFALCFEVSPGDQL 242
Query: 244 PSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYL 303
PSFFQTLVG+K+I +L GS+ SS S+LVPIV Q IP+KILFKIN+LVQHGYL
Sbjct: 243 PSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQHGYL 302
Query: 304 PGPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWK 363
PG ALDD+FF+LVDP RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK
Sbjct: 303 PGSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWK 362
Query: 364 PSVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIR 423
++S+DD LVY+HRVQITP+KVYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI
Sbjct: 363 ANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIY 422
Query: 424 SSDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREG 483
SSDL+PR S +K ERTRVYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFASREG
Sbjct: 423 SSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREG 482
Query: 484 LSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYC 543
L+A+DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPDVEV RK IKYC
Sbjct: 483 LNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNIKYC 542
Query: 544 FSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKY 603
FSDGIGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKY
Sbjct: 543 FSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKY 602
Query: 604 MSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKAL 663
MSLDTQLDVLSWSKY PC+LNRQVINLLSTLGIQD VFVK+Q++A+ QLDSIL+D SKAL
Sbjct: 603 MSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPSKAL 662
Query: 664 EVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMM 723
EVLELMSPGEMT++LK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVP+GRTMM
Sbjct: 663 EVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGRTMM 722
Query: 724 GCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLH 783
GCLDETRTLEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLH
Sbjct: 723 GCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLH 782
Query: 784 HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHD 843
HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HD
Sbjct: 783 HMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHD 842
Query: 844 VTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGV 903
VTIEEVQEY ANYMVNDGLGGIANAHTVFADK P+KAMS+ECIELAKLFSIAVDFPKTGV
Sbjct: 843 VTIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPKTGV 902
Query: 904 PANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCY 963
PANLP SLRV EYPDFMEKP++ +Y S GV GKLFRGVKDVSS+IN IETFTREVA+KCY
Sbjct: 903 PANLPRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAAKCY 962
Query: 964 DPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDM 1023
DPDMEV GF+NYL EAFD+K+ YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDM
Sbjct: 963 DPDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDM 1022
Query: 1024 EQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGM 1083
EQIS+AM+SLRKEARGWFNEKGSK Y+++KD+++ +AKASAWY+VTYHPN+WG YNEGM
Sbjct: 1023 EQISLAMRSLRKEARGWFNEKGSKSIYNNNKDEDDEYAKASAWYHVTYHPNFWGRYNEGM 1082
Query: 1084 QRDHFLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
QRDHFLSFPWCVS+KL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 QRDHFLSFPWCVSEKLIQIKREKM---ESSPVSSLIHKFGKLS 1121
BLAST of Sed0009745 vs. ExPASy TrEMBL
Match:
A0A6J1JTB1 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV=1)
HSP 1 Score: 1775.0 bits (4596), Expect = 0.0e+00
Identity = 881/1119 (78.73%), Postives = 986/1119 (88.11%), Query Frame = 0
Query: 4 TNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLA 63
TNG+TIE++GFRR+ +A EVK ++ENHTG+GT+S V ISKPKD K R TFA V F SK+A
Sbjct: 3 TNGKTIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVA 62
Query: 64 AEYVVKKAEAEERLWFRDSYLKARAVAAPMRRRNGVERMENVKGYFGNLVWEDKMRVIWK 123
AEYVV+KAEAEE LWF YLKAR V MR VERME V+ G+++ +++MRVIW+
Sbjct: 63 AEYVVEKAEAEEGLWFGRWYLKARGVEREMR----VERMEKVRVQLGSVISKEEMRVIWR 122
Query: 124 GENWGMEFGTEDRKLSFHLSHGF--DEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAG 183
GE W +E+G RKL F+LS+G D+YKMEL FENILG Q CP + SS LFLI++ G
Sbjct: 123 GEEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQL-QG 182
Query: 184 SSKIFKKTPLSSSSPN---SKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFF 243
+ +IFKK P SSSS + SK+STGF WIRDVDFTPSS IGQS +LCLE+ PGD LPSFF
Sbjct: 183 APRIFKKCPSSSSSSSQFYSKESTGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQLPSFF 242
Query: 244 QTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLPGPA 303
QTLVG+K+I +L GS+ SS S+LVPIV Q DIP+KILFKIN+LVQHGYLPG A
Sbjct: 243 QTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGYLPGSA 302
Query: 304 LDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVS 363
LDD+FF+LVDP+RFRSDYIEYALEKLF+LKECCYEP KWLK++YLS SS QLPWK ++S
Sbjct: 303 LDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPWKANIS 362
Query: 364 VDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDL 423
+DD LVY+HRVQITP+KVYFRGPEANLSNRVVRRF DDID+F+RVSFVDEELDKI SSDL
Sbjct: 363 LDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKIHSSDL 422
Query: 424 APR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSAS 483
+PR S +K ERTRVYDRI SVLRNGIVIG KKFEFLAFSASQLR+NSFWMFASREGL+A+
Sbjct: 423 SPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASREGLNAA 482
Query: 484 DIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSDG 543
DIR+WMGDF IRNVAKYAARLGQSFGSSRKTLCVE++EIE+IPD EV RK IKYCFSDG
Sbjct: 483 DIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKYCFSDG 542
Query: 544 IGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSLD 603
IGKISEA AE VAE+CGL+ HTPSAFQIRYAG+KGVVA+DPTS KKLSLR SMLKYMSLD
Sbjct: 543 IGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLKYMSLD 602
Query: 604 TQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVLE 663
TQLDVLSWSKY PC+LNRQVINLLSTLGI+D VFVK+Q++A+ QLDSIL+D SKALEVLE
Sbjct: 603 TQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKALEVLE 662
Query: 664 LMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCLD 723
LMSPGEMT++LK+MLL YNPHEEPFLNMMLRTFRADKLLDL+ KSRIFVPKGRTMMGCLD
Sbjct: 663 LMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLD 722
Query: 724 ETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMVD 783
ETRTLEYGQVF CSVPG++S+S+FVVKGKVVVAKNPCLHPGDVRV DAVDVKTLHHMVD
Sbjct: 723 ETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTLHHMVD 782
Query: 784 CVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIE 843
CVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELTCIR +KPMSYEPAPTIQL+HDVT+E
Sbjct: 783 CVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDHDVTLE 842
Query: 844 EVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANL 903
EVQEY ANYMVNDGLGGIANAHTVFAD P+KAMS+ECIELAKLFSIAVDFPKTGVPANL
Sbjct: 843 EVQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTGVPANL 902
Query: 904 PGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPDM 963
P SLRV EYPDFMEKP++ +Y+S GV GKLFRGVKDVSS+IN IETFTREVA KCYDPDM
Sbjct: 903 PRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKCYDPDM 962
Query: 964 EVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQIS 1023
EV GF+NYL EAFD+K YDFKLGNLM YYGIETEPELV GNILRMGKSFDKRNDMEQIS
Sbjct: 963 EVMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRNDMEQIS 1022
Query: 1024 IAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDH 1083
+AM+SLRKEARGWFNEK SK Y+++K ++ +AKASAWY+VTYHPN+WG YNEGMQRDH
Sbjct: 1023 LAMRSLRKEARGWFNEKASKSIYNNNKGEDNEYAKASAWYHVTYHPNFWGRYNEGMQRDH 1082
Query: 1084 FLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKFGKLS 1114
FLSFPWCVSDKL+QIKREKM SSPVSSLI+KFGKLS
Sbjct: 1083 FLSFPWCVSDKLIQIKREKM---ESSPVSSLIHKFGKLS 1113
BLAST of Sed0009745 vs. ExPASy TrEMBL
Match:
A0A6J1FF44 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 SV=1)
HSP 1 Score: 1731.5 bits (4483), Expect = 0.0e+00
Identity = 855/1113 (76.82%), Postives = 968/1113 (86.97%), Query Frame = 0
Query: 1 MTTTN--GRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRF 60
M TTN G+TIE+YGF TADEVK ++ENHTGDGT+ TV ISK D+K RFTFATV+F
Sbjct: 1 MATTNDMGKTIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQF 60
Query: 61 TSKLAAEYVVKKAEAEERLWFRDSYLKARAVAAPM----RRRNGVERMENVKGYFGNLVW 120
TSKL EYVV +A AE+RLWF SYLKAR V + R +ERMENV +G+LV
Sbjct: 61 TSKLGGEYVVAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVS 120
Query: 121 EDKMRVIWKGENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLF 180
+++M+VIWKG W +E+G RKL F+LS+ EYKMEL FENILG Q C +DR SKLF
Sbjct: 121 KEEMKVIWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLF 180
Query: 181 LIKVFAGSSKIFKKTPLSSSSP-NSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDH 240
LI++ G+ +IFKK PLSSSS +S++STGF WIRDVDFTPSS IGQS ALCLEL GD
Sbjct: 181 LIQL-QGAPRIFKKAPLSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLELSHGDQ 240
Query: 241 LPSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGY 300
LPSFFQTLVG+K+ A LQ GSS SS S+LVPI+ Q DIP+KILFKIN+L+QHGY
Sbjct: 241 LPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQHGY 300
Query: 301 LPGPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPW 360
LPGPALD+EFF+LVD +RFR DY+EYAL+KLF+ KECCYEP KWLK++YLS YSSKQLPW
Sbjct: 301 LPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQLPW 360
Query: 361 KPSVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKI 420
KP++S+DDGLVY+HRVQITP+KVYFRGPEANLSNRVVR F DD+D+F+RVSFVDEELDK+
Sbjct: 361 KPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELDKL 420
Query: 421 RSSDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASRE 480
S DL+PR SA+ RTRVYDR+ SVLRNGIVIG+KKFEFLAFSASQLR+NS WMFASR+
Sbjct: 421 HSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFASRK 480
Query: 481 GLSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKY 540
GLSA+DIR+WMGD IRNVAKYAARLGQSFGSSR+TLCVE+ EIE+I DVEV +I Y
Sbjct: 481 GLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKITY 540
Query: 541 CFSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLK 600
CFSDGIGKIS AEKVAE+CGL+ HTPSAFQIRYAG+KGVVAIDPTSTKKLSLR SMLK
Sbjct: 541 CFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSMLK 600
Query: 601 YMSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKA 660
Y SLDTQ+DVLSWSKY PC+LNRQVINLLSTLGI+D VFVKKQ+EAI QLDSIL+D S+A
Sbjct: 601 YTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSRA 660
Query: 661 LEVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTM 720
LEVLELMSPGEMT+ILKE+LLLY P+EEPFLNMMLRTFRA KLLDL+ KSRIFVPKGRTM
Sbjct: 661 LEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGRTM 720
Query: 721 MGCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTL 780
MGCLDETRTLEYGQVF CS+PG++S+S+FVVKGKVVVAKNPCLHPGDVR+ DAVDVK L
Sbjct: 721 MGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVKAL 780
Query: 781 HHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNH 840
HHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFV WDPELT I+ VKPMSYEPAPT+QL+H
Sbjct: 781 HHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQLDH 840
Query: 841 DVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTG 900
DVTIEEVQ YL YMVNDGLGGIANAHTVFADK P+KAM++ECI+LAKLFSIAVDFPKTG
Sbjct: 841 DVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPKTG 900
Query: 901 VPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKC 960
VPAN P +LRV+EYPDFMEKP +P+YVS GV GKLFRGVKDVSS++N +E F+REVA+KC
Sbjct: 901 VPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVATKC 960
Query: 961 YDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRND 1020
YDPDMEVDGF++YL EAF+FK RYDFKLGNLM YYGI+TEPEL+ GNILRM KSFDKRND
Sbjct: 961 YDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKRND 1020
Query: 1021 MEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEG 1080
MEQIS+AMKSLRKEAR WFNEKGSK AYD+D + NE +AKASAWY VTYHP+YWG YNEG
Sbjct: 1021 MEQISLAMKSLRKEARSWFNEKGSKSAYDEDDNKNEEYAKASAWYRVTYHPDYWGRYNEG 1080
Query: 1081 MQRDHFLSFPWCVSDKLLQIKREKMCMINSSPV 1103
MQRDHFLSFPWCVSDKL+QIKREK C++N SPV
Sbjct: 1081 MQRDHFLSFPWCVSDKLIQIKREKTCLVNFSPV 1112
BLAST of Sed0009745 vs. ExPASy TrEMBL
Match:
A0A6J1K1Q3 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV=1)
HSP 1 Score: 1723.0 bits (4461), Expect = 0.0e+00
Identity = 855/1118 (76.48%), Postives = 971/1118 (86.85%), Query Frame = 0
Query: 1 MTTTN--GRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRF 60
M TTN G+ IE+YGF + TADEVK ++ENHTGDGT+ TV ISKP D+K RFTFATVRF
Sbjct: 1 MATTNDMGKMIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRF 60
Query: 61 TSKLAAEYVVKKAEAEERLWFRDSYLKARAV---------AAPMRRRNGVERMENVKGYF 120
TSKL EYVV +A AE+RLWF SYLKAR V AA R G+ERMENV +
Sbjct: 61 TSKLGGEYVVAQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQW 120
Query: 121 GNLVWEDKMRVIWKGENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDR 180
G+LV ++KM+VIWK W +E+G RKL F+LS+ EYKMEL FENILG Q C DR
Sbjct: 121 GSLVSKEKMKVIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDR 180
Query: 181 SSKLFLIKVFAGSSKIFKKTPLSSSSP-NSKKSTGFPWIRDVDFTPSSSIGQSSALCLEL 240
S+LFLI++ G+ +IFKKTP SSSS +S++STGF WIRDVDFTPSS IGQS ALCLEL
Sbjct: 181 GSELFLIQL-QGAPRIFKKTPSSSSSRLSSEESTGFRWIRDVDFTPSSCIGQSFALCLEL 240
Query: 241 QPGDHLPSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSL 300
PGD LPSFF+TLVG+K+ A L PGSS SS S+LVPI+ Q DI +KILFKIN+L
Sbjct: 241 SPGDQLPSFFRTLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKINAL 300
Query: 301 VQHGYLPGPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSS 360
+QHGYLPGPALD+EFF+LVD +RFR +YIEYALEKLF+ KECCYEP KWLK++YLS YSS
Sbjct: 301 LQHGYLPGPALDNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFYSS 360
Query: 361 KQLPWKPSVSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDE 420
KQLP KP+VS+DDGLVY+HRVQITP+KVYFRGPEANLSNRVVR F DD+D+F+RVSFVDE
Sbjct: 361 KQLPCKPNVSLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDE 420
Query: 421 ELDKIRSSDLAPR-SADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWM 480
ELDK+ S DL+PR SA+ RTRVYDR+ SVLRNGIVIG+KKFEFLAFSASQLR+NS WM
Sbjct: 421 ELDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWM 480
Query: 481 FASREGLSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVR 540
FASR+GLSA+DIR+WMGDF IRNVAKYAARLGQSFGSSR+TLCVE+ EIE+IPDVEV
Sbjct: 481 FASRKGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEVET 540
Query: 541 KEIKYCFSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLR 600
+I YCFSDGIGKIS AEKVAE+CGL+ HTPSAFQIRYAG+KGVVAIDPTSTKKLSLR
Sbjct: 541 NKITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLR 600
Query: 601 DSMLKYMSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILK 660
SMLKY SLDTQ+DVLSWSKY PC+LNRQVINLLSTLGI+D VFVKKQ+EAI QLDSIL+
Sbjct: 601 KSMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILE 660
Query: 661 DSSKALEVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVP 720
D S+ALEVLELMSPGEMT+ILKE+LLLY P+EEPFLNMMLRTFRA KLLDL+ KSRIFVP
Sbjct: 661 DPSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVP 720
Query: 721 KGRTMMGCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAV 780
KGRTMMGCLDETRTLEYGQVF CS+PG++S+S+FVVKGKVVVAKNPCLHPGDVR+ DAV
Sbjct: 721 KGRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAV 780
Query: 781 DVKTLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPT 840
DVK LHHMV+CVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+ VKPMSYEPAPT
Sbjct: 781 DVKALHHMVNCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPT 840
Query: 841 IQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVD 900
++L+HDVTIEEVQEYLA YMVNDGLGGIANAHTVFADK P+KAM++ECI+LAKLFSIAVD
Sbjct: 841 MRLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVD 900
Query: 901 FPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTRE 960
FPKTGVPAN P +LRV+EYPDFMEKP +P+YVS GV GKLFRGVKDVSS++N IE F+RE
Sbjct: 901 FPKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFSRE 960
Query: 961 VASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSF 1020
VA+KCYDPDMEVDGF++YL EAF++K RYDFKLGNLM YYGI+TEPEL+ GNILRM KSF
Sbjct: 961 VATKCYDPDMEVDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSF 1020
Query: 1021 DKRNDMEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWG 1080
DKRND+EQIS+A+KSLRKEAR WFNEKGSK A D+D D +E +AKASAWY VTYHP+YWG
Sbjct: 1021 DKRNDLEQISLAIKSLRKEARSWFNEKGSKSACDED-DKDEEYAKASAWYRVTYHPDYWG 1080
Query: 1081 LYNEGMQRDHFLSFPWCVSDKLLQIKREKMCMINSSPV 1103
YNEGMQRDHFLSFPWCVSDKL+QIKREK C++N SP+
Sbjct: 1081 RYNEGMQRDHFLSFPWCVSDKLIQIKREKTCLVNLSPI 1116
BLAST of Sed0009745 vs. ExPASy TrEMBL
Match:
A0A0A0KRH3 (RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G564290 PE=3 SV=1)
HSP 1 Score: 1700.6 bits (4403), Expect = 0.0e+00
Identity = 843/1116 (75.54%), Postives = 955/1116 (85.57%), Query Frame = 0
Query: 6 GRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLAAE 65
G+TIE+YGFR TADEVK +LENHTGDGT+STV ISKPKD+K RFT TV F SKLAAE
Sbjct: 2 GKTIEIYGFRPQVTADEVKEFLENHTGDGTVSTVRISKPKDEKARFTSVTVLFKSKLAAE 61
Query: 66 YVVKKAEAEER-LWFRDSYLKARAV--AAPMRRRNGV---ERMENVKGYFGNLVWEDKMR 125
Y+V K+ EER LWF SYLKAR + A + GV ERME+VKG+ G+++ KMR
Sbjct: 62 YIVAKSTTEERKLWFESSYLKARELEKAVVAKETKGVLEMERMEDVKGHLGSMISNGKMR 121
Query: 126 VIWKGENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLFLIKVF 185
VIW+GE W +EFG RKL F+LS+ DEYKMEL FENIL +F CPL+ SK FLI++
Sbjct: 122 VIWEGEKWSVEFGNGFRKLWFYLSYEVDEYKMELCFENILSVEFRCPLNEPSKFFLIQL- 181
Query: 186 AGSSKIFKKTPLSSSSP-NSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFF 245
G+ +IF+KTP SSSSP S KST F WIRDVDFTPSS IGQS +CL+L P HLP FF
Sbjct: 182 QGAPRIFRKTPSSSSSPLYSNKSTSFRWIRDVDFTPSSCIGQSFTICLQLSPSHHLPPFF 241
Query: 246 QTLVGFKQICARLELQPGSSSSSPSDLVPIV---QRLDIPFKILFKINSLVQHGYLPGPA 305
QTLVG+K A L GSS S S+LVPI+ Q DI +KILFKIN+L+QHGYL GP
Sbjct: 242 QTLVGYKVTYAPFILHKGSSLVSNSNLVPIITPPQAFDISYKILFKINALLQHGYLSGPT 301
Query: 306 LDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVS 365
LDDEFF+LVD +RF SDYI++ALEKLFNLKECCY+P KWLK++YLS Y+S QLPWK ++S
Sbjct: 302 LDDEFFRLVDSSRFHSDYIDHALEKLFNLKECCYKPQKWLKDQYLSYYTSNQLPWKSNIS 361
Query: 366 VDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDL 425
+DDGLVY+HRVQITP KVYF GPEANLSNRVVRRF DID+F+RVSFVDEELDK+ S DL
Sbjct: 362 LDDGLVYVHRVQITPLKVYFCGPEANLSNRVVRRFIGDIDNFLRVSFVDEELDKLHSIDL 421
Query: 426 APRSA--DKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSA 485
APRS+ + S+RTRVYDR+ SVL+NGIVIG KKFEFLAFSASQLR+NSFWMFASREGLSA
Sbjct: 422 APRSSSHENSQRTRVYDRVVSVLKNGIVIGNKKFEFLAFSASQLRENSFWMFASREGLSA 481
Query: 486 SDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSD 545
+DIR+WMGDF +IRNVAKYAARLGQSFGSSRKTLCVE+ EIE+IPDVEV RK I YCFSD
Sbjct: 482 ADIREWMGDFHHIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVIPDVEVERKNIMYCFSD 541
Query: 546 GIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSL 605
GIGKIS+ A+KVAE+CGL+ HTPSAFQIRYAG+KGVVAIDPTS KKLSLR+SMLKYMSL
Sbjct: 542 GIGKISKTLAKKVAEKCGLINHTPSAFQIRYAGYKGVVAIDPTSKKKLSLRNSMLKYMSL 601
Query: 606 DTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVL 665
DTQLDVLSWSKY PC+LNRQVINLLSTLGI D VFVKKQ+EAI QLDSIL+D S+ALEVL
Sbjct: 602 DTQLDVLSWSKYQPCFLNRQVINLLSTLGIGDDVFVKKQKEAIDQLDSILEDPSRALEVL 661
Query: 666 ELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCL 725
ELMSPGEMT+ILKE+L Y P+EEPFLNMMLRTFRA+KLLDLK KSRIFVP+GRTM+GCL
Sbjct: 662 ELMSPGEMTSILKELLSFYMPNEEPFLNMMLRTFRANKLLDLKTKSRIFVPEGRTMIGCL 721
Query: 726 DETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMV 785
DETRTLEYGQVF CSVP ++S+ +FVVKGK+VVAKNPCLHPGDVRV DAVDVK LHHMV
Sbjct: 722 DETRTLEYGQVFVHCSVPRRSSEGNFVVKGKIVVAKNPCLHPGDVRVLDAVDVKALHHMV 781
Query: 786 DCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTI 845
DCVVFPQKGKRPHPNECSGSDLDGDLYF WD ELTCI+ VKPM+YEPAPTIQLNHDVTI
Sbjct: 782 DCVVFPQKGKRPHPNECSGSDLDGDLYFACWDLELTCIKQVKPMNYEPAPTIQLNHDVTI 841
Query: 846 EEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPAN 905
EE+QEY ANYMVNDG+G IANAHTVFADK+ +KAMS ECI+LAKLFSIAVDFPKTGVPAN
Sbjct: 842 EEIQEYFANYMVNDGIGAIANAHTVFADKNSKKAMSVECIKLAKLFSIAVDFPKTGVPAN 901
Query: 906 LPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPD 965
LP +LRV EYPDFM+KP++P+YVS GV GKLFRGVKDVSS++N E FTREVA+K YDPD
Sbjct: 902 LPRNLRVHEYPDFMDKPNKPTYVSNGVLGKLFRGVKDVSSDVNTFEIFTREVATKYYDPD 961
Query: 966 MEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQI 1025
MEVDGF+ YL EAFD+KT+YDFKLGNLM YYGI+TEPELV GN+L+M KSFDKRND+EQI
Sbjct: 962 MEVDGFEKYLREAFDYKTKYDFKLGNLMDYYGIKTEPELVSGNVLKMAKSFDKRNDLEQI 1021
Query: 1026 SIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRD 1085
+ AMKSLRKE R WFNE SK YDD +D+ +AKASAWYYVTYHP+YWG YNEGMQRD
Sbjct: 1022 TFAMKSLRKEVRSWFNENESKFTYDDIEDE---YAKASAWYYVTYHPDYWGCYNEGMQRD 1081
Query: 1086 HFLSFPWCVSDKLLQIKREKMCMINSSPVSSLINKF 1110
HFLSFPWCV+DKL+QIKR+KM + NS PVSSL + F
Sbjct: 1082 HFLSFPWCVADKLIQIKRDKMTLKNSYPVSSLFHNF 1113
BLAST of Sed0009745 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 610/1102 (55.35%), Postives = 782/1102 (70.96%), Query Frame = 0
Query: 6 GRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSKLAAE 65
G+TI+++GF +A+EVK +LE TG GT+ + + +PK R +A V+FTS+
Sbjct: 2 GKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPR-VYAIVQFTSERHTR 61
Query: 66 YVVKKAEAEERLWFRDSYLKARAVAAPM--RRRNGVERMENVKGYFGNLVWEDKMRVIWK 125
++ A ERL++ SYLKA V + + R + + +K +FG V K +W
Sbjct: 62 LII--TAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWS 121
Query: 126 GENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSS 185
++ + FG RKL F S +Y++EL +ENI + P RSSK +I+V G+
Sbjct: 122 AQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVI-GAP 181
Query: 186 KIFKKTP-----LSSSSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQPGDHLPSFF 245
KIF+K L + WIR DFT SS IGQS+A CLEL ++P F
Sbjct: 182 KIFEKEDQPINLLFGIMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFR 241
Query: 246 QTLVGF-KQICARLELQPGSS-SSSPSDLVPIVQ---RLDIPFKILFKINSLVQHGYLPG 305
+ + + + ++ GSS SS+ + LVP+V +PF+ILFK+N+LVQ+ L G
Sbjct: 242 ENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSG 301
Query: 306 PALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPS 365
PALD +F++L++ ++ I++ LEKLF+L ECCYEP WL+++Y S +LP P+
Sbjct: 302 PALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPT 361
Query: 366 VSVDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSS 425
+S+DDGLVY++RVQ+TP++VYF GPE N+SNRV+R +S I++F+RVSFVDE+L+K+RS
Sbjct: 362 ISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSM 421
Query: 426 DLAPRSADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSA 485
DL+PRS+ + RT++YDRI+SVLR+GIVIG+KKFEFLAFS+SQLR+NS WMFA + ++A
Sbjct: 422 DLSPRSSTQ-RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 481
Query: 486 SDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEIIPDVEVVRKEIKYCFSD 545
+ IR WMGDF +IRNVAKYAARLGQSF SSR+TL V EIE+IPDVE++ +Y FSD
Sbjct: 482 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFSD 541
Query: 546 GIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAIDPTSTKKLSLRDSMLKYMSL 605
GIGKIS A KVA +CGL +PSAFQIRY G+KGVVA+DP S+KKLSLR SM K+ S
Sbjct: 542 GIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFESE 601
Query: 606 DTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVL 665
+T+LDVL+WSKY PCY+NRQ+I LLSTLG+ D VF KKQ E + +LD+IL +A E L
Sbjct: 602 NTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEAL 661
Query: 666 ELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGC 725
LM+PGE TNILK ++L Y P EPFL+MML+ FRA KLL+L+ K+RIF+ GR+MMGC
Sbjct: 662 GLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGC 721
Query: 726 LDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHM 785
LDETRTLEYGQV Q S P + + F++ G VVVAKNPCLHPGDVRV AV+V L+HM
Sbjct: 722 LDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNVPALNHM 781
Query: 786 VDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVT 845
VDCVVFPQKG RPHPNECSGSDLDGD+YFV WD EL RT +PM Y P PT L+HDVT
Sbjct: 782 VDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQILDHDVT 841
Query: 846 IEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELAKLFSIAVDFPKTGVPA 905
IEEV+EY ANY+VND LG IANAHT FADK P KA S CIELAK FS AVDFPKTGV A
Sbjct: 842 IEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFPKTGVAA 901
Query: 906 NLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDVSSNINIIETFTREVASKCYDP 965
+P L VKEYPDFMEKP +P+Y SK V GKLFR VK+ + + I++FT +VASK YD
Sbjct: 902 VIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVASKSYDK 961
Query: 966 DMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGGNILRMGKSFDKRNDMEQ 1025
DMEVDGF+ Y+ EAF K YDFKLGNLM YYGI+TE E++ G I+RM KSF KR D E
Sbjct: 962 DMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAES 1021
Query: 1026 ISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQR 1085
I A+++LRKE FN ++++NE AKASAWY+VTYH +YWGLYNEG+ R
Sbjct: 1022 IGRAVRALRKETLSLFNA--------SEEEENES-AKASAWYHVTYHSSYWGLYNEGLNR 1081
Query: 1086 DHFLSFPWCVSDKLLQIKREKM 1095
DHFLSF WCV DKL++IK+ +
Sbjct: 1082 DHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of Sed0009745 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 745.3 bits (1923), Expect = 6.9e-215
Identity = 441/1139 (38.72%), Postives = 667/1139 (58.56%), Query Frame = 0
Query: 2 TTTNGRTIELYGFRRDTTADEVKNYLENHTGDGTISTVNISKPKDKKTRFTFATVRFTSK 61
TTTN T+++ + ADE+ +LE H G+ T+ + I +D FA V+FT+
Sbjct: 5 TTTNRSTVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT- 64
Query: 62 LAAEYVVKKAEAEERLWFRDSYLKAR-----AVAAPMRRRNGVERMENVKGYFGNLVWED 121
L + + ++ +L F+ L+ + P+ R ++ + G+ + E
Sbjct: 65 LEVKSRAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPES--DEK 124
Query: 122 KMRVIWKGENWGMEFGTEDRKLSFHLSHGFDEYKMELPFENILGYQFYCPLDRSSKL--F 181
+ + K + TE R++ F + D YK+E+ FE+I+ C +S++ F
Sbjct: 125 RFCALEKWDGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAF 184
Query: 182 LIKVFAGSSKIFKKTPL-------SSSSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLE 241
L+K+ G K+FK+ + S K+ F WIR DF+ S SIG S+ CLE
Sbjct: 185 LLKLKYG-PKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLE 244
Query: 242 LQPGDHLPSFFQTLVGFKQICARLELQPGSSSSSPSDLVPIVQR----LDIPFKILFKIN 301
+ G + F L +++ L G + +S + +VP++ L+ P++ILF++N
Sbjct: 245 VHNGSTMLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLN 304
Query: 302 SLVQHGYLP-GPALDDEFFQLVDPNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSL 361
+LV + A D E +++ + + L+KL CY+P ++K + S+
Sbjct: 305 ALVHAQKISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSV 364
Query: 362 YSSKQLPWKPSVS----VDDGLVYIHRVQITPSKVYFRGPEANLSNRVVRRFSDDIDSFI 421
K++ P+ + + ++ R +TPSK+Y GPE +N VV+ F++ + F+
Sbjct: 365 V--KKMKHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFM 424
Query: 422 RVSFVDEELDKIRSSDLAPRSAD----KSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSA 481
RV+FV+E+ K+ ++ L+ S + K RT +Y+R+ S+L GI +G K+FEFLAFSA
Sbjct: 425 RVTFVEEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSA 484
Query: 482 SQLRDNSFWMFASREGLSASDIRDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEI 541
SQLR NS WMFAS E + A DIR+WMG F IR+++K AAR+GQ F +SR+TL V ++
Sbjct: 485 SQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDV 544
Query: 542 EIIPDVEVVRKEIKYCFSDGIGKISEAAAEKVAEECGLLCHTPSAFQIRYAGFKGVVAID 601
E IPD+EV YCFSDGIGKIS A A++VA++CG L H PSAFQIRY G+KGV+A+D
Sbjct: 545 EQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQKCG-LSHVPSAFQIRYGGYKGVIAVD 604
Query: 602 PTSTKKLSLRDSMLKYMSLDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEE 661
+S +KLSLRDSMLK+ S + L+V W++ PC+LNR++I LLSTLGI+D +F Q
Sbjct: 605 RSSFRKLSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAV 664
Query: 662 AIYQLDSILKDSSKALEVLELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLL 721
+ L ++L+D AL VL+ +S N+L +MLL Y P EP+L+MMLR +L
Sbjct: 665 HLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLS 724
Query: 722 DLKNKSRIFVPKGRTMMGCLDETRTLEYGQVFAQCSVP------------GKASKSSFVV 781
+LK++ RI VPKGR ++GC+DE LEYGQV+ + ++ K + + VV
Sbjct: 725 ELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVV 784
Query: 782 KGKVVVAKNPCLHPGDVRVFDAVDVKTLHH--MVDCVVFPQKGKRPHPNECSGSDLDGDL 841
GKVVV KNPCLHPGD+RV DA+ +DC++FPQKG+RPHPNECSG DLDGD
Sbjct: 785 IGKVVVTKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQ 844
Query: 842 YFVSWDPELTCIRTVKPMSYEPAPTIQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVF 901
+FVSWD ++ PM Y + ++HDVT+EE+ ++ +YM++D LG I+ AH V
Sbjct: 845 FFVSWDEKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVH 904
Query: 902 ADKSPQKAMSSECIELAKLFSIAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKG 961
AD+ P+KA S +C+ELA L S AVDF KTG PA +P +L+ +E+PDF+E+ +P+Y+S+
Sbjct: 905 ADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISES 964
Query: 962 VFGKLFRGVKDVSSNINIIETFTREVASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGN 1021
VFGKL+R VK S++ + + YD +E GF++++ A + Y KL +
Sbjct: 965 VFGKLYRAVK---SSLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTS 1024
Query: 1022 LMGYYGIETEPELVGGNILRMGKSFDKRND------MEQISIAMKSLRKEARGWFNEKGS 1081
LM YYG E E++ G IL+ + + R++ ++I++++K L KEA GWF +
Sbjct: 1025 LMIYYGAANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEK--- 1084
Query: 1082 KCAYDDDKDDNEGFAKASAWYYVTYHPNYWGLYNEGMQRDHFLSFPWCVSDKLLQIKRE 1093
C +D+ + ASAWYYVTY+PN+ ++ FLSFPW V D LL IK E
Sbjct: 1085 SC-----EDEQQKKKLASAWYYVTYNPNHRD------EKLTFLSFPWIVGDVLLDIKAE 1116
BLAST of Sed0009745 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 571.2 bits (1471), Expect = 1.8e-162
Identity = 400/1081 (37.00%), Postives = 570/1081 (52.73%), Query Frame = 0
Query: 94 RRRNGVE-RMENVKGYFGNLVWEDKMRVIWKGENWGMEFGTE----DRKLSFHLSHGFD- 153
RRR V ++ + G LV D V W+ E G++F + K F S F
Sbjct: 123 RRRTTVPYKLAGITLEIGTLVSRDDFFVSWRAE--GVDFLVDPFDNTCKFCFRKSTAFSF 182
Query: 154 -----------EYKMELPFENILGYQFYCPLDRSSKLFLIKVFAGSSKIFKKT---PLSS 213
+YK+EL +I + Y L LI A S +++ +T +
Sbjct: 183 KDAVMHAVINCDYKLELLVRDIQTVRQYKTL---HGFVLILQLASSPRVWYRTADDDIYD 242
Query: 214 SSPNSKKSTGFPWIRDVDFTPSSSIGQSSALCLELQP------GDHLPSFFQTLVGFKQI 273
+ P PWIR DFT +IG+ + + + P L F V +++
Sbjct: 243 TVPGDLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERV 302
Query: 274 --CARLELQPGSSSSSPSDLVPIVQRLDIPFKILFKINSLVQHGYLPGPALDDEFFQLVD 333
R+ +P I + I F+I+F +NS++ G L + FF L+
Sbjct: 303 RWPPRIRNEPCFGEPVSDHFFCIHHKEGISFEIMFLVNSVLHRGVFNQFQLTERFFDLL- 362
Query: 334 PNRFRSDYIEYALEKLFNLKECCYEPHKWLKEKYLSLYSSKQLPWKPSVSVDDGLVYIHR 393
D +L+ L K ++ +K LK + + +L S D + I R
Sbjct: 363 -RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRR 422
Query: 394 VQITPSKVYFRGPEANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDL----AP---- 453
+ ITP++ Y PE LSNRV+RR+ + F+RV+F+DE + I S+ L AP
Sbjct: 423 LVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKD 482
Query: 454 -RSADKSERTRVYDRIFSVLRNGIVIGEKKFEFLAFSASQLRDNSFWMFASREGLSASDI 513
S+ S++T V+ R+ S+L +G + +K+ FLAFSA+QLRD S W FA SDI
Sbjct: 483 LTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDI 542
Query: 514 RDWMGDFSNIRNVAKYAARLGQSFGSSRKTLCVEKSEIEI-IPDVEVVRKEIKYCFSDGI 573
+ WMG F + +NVAK AAR+G F S+ T+ V E++ +PD+E Y FSDGI
Sbjct: 543 KTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIE----RNGYVFSDGI 602
Query: 574 GKISEAAAEKVAEECGLLCH-TPSAFQIRYAGFKGVVAIDPTSTK--KLSLRDSMLKYMS 633
G I+ A++V E+ L H +P A+QIRYAGFKGVVA P+ + +L+LRDSM K+ S
Sbjct: 603 GTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFS 662
Query: 634 LDTQLDVLSWSKYHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEV 693
T L++ SW+++ P +LNRQ+I LLS LG+ D +F QE +Y+L+ IL D+ A EV
Sbjct: 663 KHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEV 722
Query: 694 LELMSPGEMTNILKEMLLL-YNPHEEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMG 753
L S E N ML + P EP L ML + R +L L+ KSRIFV GR +MG
Sbjct: 723 L-TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMG 782
Query: 754 CLDETRTLEYGQVFAQCSVPG------------KASKSSF-VVKGKVVVAKNPCLHPGDV 813
CLDE LE+GQ F Q S P K +K+ VVKG V +AKNPCLHPGDV
Sbjct: 783 CLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDV 842
Query: 814 RVFDAVDVKTLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPEL--TCIRTVKP 873
R+ +AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV+WD +L ++
Sbjct: 843 RILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPNRKSYPA 902
Query: 874 MSYEPAPTIQLNHDVTIEEVQEYLANYMVNDGLGGIANAHTVFADKSPQKAMSSECIELA 933
M Y+ A L V +++ ++ A + N+ LG I NAH V AD+S AM EC+ LA
Sbjct: 903 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 962
Query: 934 KLFSIAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVSKGVFGKLFRGVKDV-SSNI 993
+L + AVDFPKTG ++P L+ K YPDFM K +Y S + G+L+R VK+V +
Sbjct: 963 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1022
Query: 994 NIIETFTREVASKCYDPDMEVDGFQNYLGEAFDFKTRYDFKLGNLMGYYGIETEPELVGG 1053
+ + ++ YD +E+ GF++ + EA+ K YD +L L+G Y ++ E E+V G
Sbjct: 1023 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1082
Query: 1054 NILRMGKSFDKRND--MEQISIAMKSLRKEARGWFNEKGSKCAYDDDKDDNEGF-AKASA 1106
+I M K K+ E++ + SL+KE R F E +++ N + KASA
Sbjct: 1083 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILYEKKASA 1142
BLAST of Sed0009745 vs. TAIR 10
Match:
AT2G19930.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 124.4 bits (311), Expect = 5.7e-28
Identity = 183/727 (25.17%), Postives = 300/727 (41.27%), Query Frame = 0
Query: 329 YEPHKW--LKEKYLSLYSSKQLPWKPSVSVDDGLVYIHRVQITPSKVY-------FRGPE 388
YE W L Y + L W D G + ++ + P+ Y +GP
Sbjct: 174 YEAAVWDRLGRHYCPQKDRRMLQW------DSGKTHYYQCNVAPNGSYTFKVLSALQGPL 233
Query: 389 ANLSNRVVRRFSDDIDSFIRVSFVDEELDKIRSSDLAPRSADKSERTRVYDRIFS---VL 448
+ + + D D+ + V F D KS T D F+ +
Sbjct: 234 LEHTGTHLHKVLGD-DNVLTVKFAD---------------VQKSSSTYSIDHYFTYKGIA 293
Query: 449 RNGIVIGEKKFEFLAF------------SASQLR-------DNSFWMFASREGLSASDIR 508
+NGI+IG ++++F F S +++ +F+ + L+ I
Sbjct: 294 KNGIMIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQNPYILTGKSIY 353
Query: 509 DWMGDFSNIR---NVAKYAARLGQSFGSSRKTLCVEKSEI--EIIPDV--------EVVR 568
+ F ++ +A Y AR S KTL V+ + I + I D+ +V+
Sbjct: 354 EARMHFMHVHRAPTLANYMARFSLIL-SKTKTLEVDMTGITFDQIDDIHCHDQDGKDVLD 413
Query: 569 KEIKYCF-SDGIGKISEAAA---------------EKVAEECGLLCHTPSAFQIRYAGF- 628
K K C SDG G ISE A E + E C F++ Y G+
Sbjct: 414 KNKKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDGYA 473
Query: 629 -KGVVAID-PTSTKKLSLRDSMLKY--------MSLDTQLDVLSWSK-YHPCYLNRQVIN 688
KG ++ + + +R SM+K S L+V++ S L++ ++
Sbjct: 474 VKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVA 533
Query: 689 LLSTLGIQDHVFVKKQEEAIYQLDSILKDSSKALEVLELMSPGEMTN-ILKEMLLLYNPH 748
LLS GI + F+ + + SI + AL ++ GEM + +M+L+ P
Sbjct: 534 LLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNA--ALNYGEMDDQNAAQMILVGIPL 593
Query: 749 EEPFLNMMLRTFRADKLLDLKNKSRIFVPKGRTMMGCLDETRTLEYGQVFAQCSVPGKAS 808
+EP L L + DLK ++ V + +MG +D T L+ +V C +
Sbjct: 594 DEPHLKNYLSILLKTEKNDLK-AGKLPVTESYYLMGTVDPTGALKEDEV---CVI----- 653
Query: 809 KSSFVVKGKVVVAKNPCLHPGDVRVFDAVDVKTLHHMVD----CVVFPQKGKRPHPNECS 868
S + G+V+V +NP LH GD+ + A VK L V V FPQKG R +E +
Sbjct: 654 LESGQISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDEIA 713
Query: 869 GSDLDGDLYFVSWDPELTCIRTVKP----MSYEP--------APTIQLNHDVTIEEVQEY 928
G D DGD+YF+S +PEL + KP +S P AP+ QL+ + EE+ E
Sbjct: 714 GGDFDGDMYFISRNPEL--LENFKPSEPWVSLTPPSKSNSGRAPS-QLSPEELEEELFEM 773
Query: 929 L--ANYMVNDGLGGIANAHTVFA--------DKSPQKA-MSSECIELAKLFSIAVDFPKT 949
A + ++ +G A++ D++ +KA M + +EL ++ A+D PK
Sbjct: 774 FLTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKK 833
BLAST of Sed0009745 vs. TAIR 10
Match:
AT2G19910.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 107.8 bits (268), Expect = 5.6e-23
Identity = 104/399 (26.07%), Postives = 175/399 (43.86%), Query Frame = 0
Query: 585 DSMLKYMSLDTQLDVLSWSK-YHPCYLNRQVINLLSTLGIQDHVFVKKQEEAIYQLDSIL 644
D L +S L+V++ S L+R ++ LLS G+ + F+ + + +I
Sbjct: 491 DRTLSNLSTFNSLEVVTTSNPPRKARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIF 550
Query: 645 KDSSKALEVLELMSPGEMTNILKEMLLLYNPHEEPFLNMMLRTFRADKLLDLKNKSRIFV 704
A + + T +M+L+ P +EP+L L + LK R +
Sbjct: 551 YSERAAFKAAINYGDDQYT---ADMILVGIPLDEPYLKDRLSYLLKTERNALK-AGRFPI 610
Query: 705 PKGRTMMGCLDETRTLEYGQVFAQCSVPGKASKSSFVVKGKVVVAKNPCLHPGDVRVFDA 764
+ +MG +D T L+ ++ C + S + G V+V +NP LH GD+ V A
Sbjct: 611 DESYYIMGTVDPTGELKENEI---CVI-----LHSGQISGDVLVYRNPGLHFGDIHVLKA 670
Query: 765 VDVKTLHHMVD----CVVFPQKGKRPHPNECSGSDLDGDLYFVSWDPELTCIRTVKP--- 824
VK L V V FPQKG R +E +G D DGD+YF+S +P+L + KP
Sbjct: 671 TYVKALEDYVGNAKFAVFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKL--LEHFKPSEP 730
Query: 825 --MSYEPAPTI--QLNHDVTIEEVQEYL------ANYMVNDGLG-------GIANAHTVF 884
S +P+ + +++ EE++E L A + D +G GI +
Sbjct: 731 WVSSSKPSKIYCGRKPSELSEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTL 790
Query: 885 ADKSPQK--AMSSECIELAKLFSIAVDFPKTGVPANLPGSLRVKEYPDFMEKPHRPSYVS 944
D+S ++ ++L ++ A+D PK G +LP L +K +P +ME+ + + S
Sbjct: 791 GDESAKEKYERKKNILKLIDIYYDALDAPKKGAKVDLPPDLEIKNFPHYMERDPKRDFRS 850
Query: 945 KGVFGKLFRGVKDVSSNINIIETFTREVASKCYDPDMEV 957
+ G +F D + N E E++ Y D V
Sbjct: 851 TSILGLIF----DTVDSHNAEEPPPSEISKLWYFEDEPV 871
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_023524039.1 | 0.0e+00 | 78.83 | probable RNA-dependent RNA polymerase 1 isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_023524048.1 | 0.0e+00 | 78.34 | probable RNA-dependent RNA polymerase 1 isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
KAG7032582.1 | 0.0e+00 | 78.31 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. argyrospe... | [more] |
XP_022923210.1 | 0.0e+00 | 78.45 | probable RNA-dependent RNA polymerase 1 [Cucurbita moschata] | [more] |
XP_022990904.1 | 0.0e+00 | 78.73 | probable RNA-dependent RNA polymerase 1 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9LQV2 | 0.0e+00 | 55.35 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
Q0DXS3 | 2.6e-259 | 61.44 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
O82504 | 9.7e-214 | 38.72 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
Q7XM31 | 6.5e-202 | 40.44 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
Q9SG02 | 2.5e-161 | 37.00 | RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EB47 | 0.0e+00 | 78.45 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 ... | [more] |
A0A6J1JTB1 | 0.0e+00 | 78.73 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV... | [more] |
A0A6J1FF44 | 0.0e+00 | 76.82 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 ... | [more] |
A0A6J1K1Q3 | 0.0e+00 | 76.48 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV... | [more] |
A0A0A0KRH3 | 0.0e+00 | 75.54 | RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G564290 PE=3 SV=... | [more] |