Sed0009686 (gene) Chayote v1

BLAST of Sed0009686 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 733.0 bits (1891), Expect = 1.6e-207
Identity = 370/472 (78.39%), Postives = 398/472 (84.32%), Query Frame = 0

Query: 1   MVYVSPFLTLIIFALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTPSQ 60
           M+ +SPF   I     L + NA A D                 YLKLPLLH  P+S+PSQ
Sbjct: 1   MLPISPFFHFIFLFFFLTNFNATAAD-----------------YLKLPLLHKPPFSSPSQ 60

Query: 61  TLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTG 120
            LSSDTHRLSLLF      R N T+KSPLISGASTGSGQYFV+LRLGTPPQSLLLVADTG
Sbjct: 61  ALSSDTHRLSLLF-----SRPNPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTG 120

Query: 121 SDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHS 180
           SDLVWVKCSAC NCSHHPPSTAFLPRHSS+FSPFHC+ P CRL+PHAP  H CNHTR HS
Sbjct: 121 SDLVWVKCSACRNCSHHPPSTAFLPRHSSSFSPFHCFDPHCRLLPHAP-PHLCNHTRFHS 180

Query: 181 PCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGAR 240
           PC +LYSYADGSLSSGFFSKETTT  TLSGS+ H   LSFGCGFRISGPSVSGA+F+GAR
Sbjct: 181 PCRFLYSYADGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGAR 240

Query: 241 GVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISY 300
           GVMGLGRG ISFSSQLGRRFG+ FSYCLMDYT+SPPPTSYLMIG G R +P TNA++ISY
Sbjct: 241 GVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISY 300

Query: 301 TPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAY 360
           TPL INPLSPTFYYIA++ ITVDG KLPI+PAVWA+D QGNGGTVVDSGTTLTYL + AY
Sbjct: 301 TPLQINPLSPTFYYIAIHSITVDGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAY 360

Query: 361 VEVLKAVRRRVKLPSLAELTPGFDLCVN---SSGRPSLPRLRFRLGGGAVFAPPPRNYFL 420
            EVLKAVRRRVKLPS +ELTPGFDLCVN   SS RPSLPRLRFRLGGGAVFAPPPRNYFL
Sbjct: 361 DEVLKAVRRRVKLPSASELTPGFDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFL 420

Query: 421 ETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           ETEERV+CLAIRPVD+GNGFSVIGNLMQQGFLLEFDKD +RLGFSRRGCGLP
Sbjct: 421 ETEERVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

BLAST of Sed0009686 vs. NCBI nr
Match: XP_004143702.1 (aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical protein Csa_000435 [Cucumis sativus])

HSP 1 Score: 724.9 bits (1870), Expect = 4.3e-205
Identity = 361/471 (76.65%), Postives = 400/471 (84.93%), Query Frame = 0

Query: 4   VSPFLTLIIF--ALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTPSQT 63
           +SPF  LI+     L   PN NA  +A           A  ++LKLPLLH  P+S+PSQ+
Sbjct: 6   ISPFFLLILLFSFFLTHLPNPNATAVA-----------APADFLKLPLLHKPPFSSPSQS 65

Query: 64  LSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTGS 123
           LSSDTHRLSLLF      R N T+KSPLISGASTGSGQYFV++RLGTPPQSLLLVADTGS
Sbjct: 66  LSSDTHRLSLLF-----SRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGS 125

Query: 124 DLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSP 183
           DLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFHC+ P CRL+PHAP  H CNHTR HSP
Sbjct: 126 DLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP-HHLCNHTRLHSP 185

Query: 184 CHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGARG 243
           C +LYSYADGSLSSGFFSKETTT  +LSGS+ H   LSFGCGFRISGPSVSGA+FNGARG
Sbjct: 186 CRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARG 245

Query: 244 VMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYT 303
           VMGLGRG ISFSSQLGRRFG+ FSYCLMDYT+SPPPTS+LMIG GL  +P TNA++ISYT
Sbjct: 246 VMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYT 305

Query: 304 PLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYV 363
           PL INPLSPTFYYI ++ IT+DG KLPI+PAVW ID+QGNGGTVVDSGTTLTYL + AY 
Sbjct: 306 PLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYE 365

Query: 364 EVLKAVRRRVKLPSLAELTPGFDLCVNSSG---RPSLPRLRFRLGGGAVFAPPPRNYFLE 423
           EVLK+VRRRVKLP+ AELTPGFDLCVN+SG   RPSLPRLRFRLGGGAVFAPPPRNYFLE
Sbjct: 366 EVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLE 425

Query: 424 TEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           TEE V+CLAIR V++GNGFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 426 TEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sed0009686 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 366/472 (77.54%), Postives = 401/472 (84.96%), Query Frame = 0

Query: 1   MVYVSPFLTLIIFALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTPSQ 60
           M+ VSPF   I+    L              AD+ ++A A  +YLKLPLLH NP+S+PSQ
Sbjct: 5   MLSVSPFFHFILLLFSL--------------ADL-LNAAAAADYLKLPLLHKNPFSSPSQ 64

Query: 61  TLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTG 120
            LSSDTHRLSLLF S  RRR N T+KSPLISGASTGSGQYFV+LR+GTPPQSLLLVADTG
Sbjct: 65  ALSSDTHRLSLLF-SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTG 124

Query: 121 SDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHS 180
           SDLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFHC+ P CRL+PHAP  H CNHTR HS
Sbjct: 125 SDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP-PHLCNHTRLHS 184

Query: 181 PCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGAR 240
           PC +LY+YADGS SSGFFSKETTT  TLSGS+T    LSFGCGFRISGPSVSGA+FNGAR
Sbjct: 185 PCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGAR 244

Query: 241 GVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISY 300
           GVMGLGRGPISFS+QLGRRFG+ FSYCLMDYT+SPPPTSYLMIG GLR +P TNA++ISY
Sbjct: 245 GVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISY 304

Query: 301 TPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAY 360
           TPLLINPLSPTFYYIAV  ITVDG KLPI+P VWAID+QGNGGTVVDSGTTLTYL E AY
Sbjct: 305 TPLLINPLSPTFYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAY 364

Query: 361 VEVLKAVRRRVKLPSLAELTPGFDLCV---NSSGRPSLPRLRFRLGGGAVFAPPPRNYFL 420
            EVLKAVR+RVKLP+ AELTPGFDLCV   N S RPSLPR+RF+LG GAVFAPP RNYFL
Sbjct: 365 KEVLKAVRQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFL 424

Query: 421 ETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           ETEE V+CL+IR V+ GNGFSVIGNLMQQGFLLEFDK+ SRLGFSRRGCGLP
Sbjct: 425 ETEEGVMCLSIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sed0009686 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 357/437 (81.69%), Postives = 388/437 (88.79%), Query Frame = 0

Query: 36  VDADAEVEYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGAST 95
           ++A A  +YLKLPLLH NP+S+PSQ LSSDTHRLSLLF S  RRR N T+KSPLISGAST
Sbjct: 25  LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF-SALRRRPNPTLKSPLISGAST 84

Query: 96  GSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFH 155
           GSGQYFV+LR+GTPPQSLLLVADTGSDLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFH
Sbjct: 85  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 144

Query: 156 CYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHT 215
           C+ P CRL+PHAP  H CNHTR HSPC +LY+YADGS SSGFFSKETTT  TLSGS+T  
Sbjct: 145 CFDPHCRLLPHAP-PHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRL 204

Query: 216 ASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISP 275
             LSFGCGFRISGPSVSGA+FNGARGVMGLGRGPISFS+QLGRRFG+ FSYCLMDYT+SP
Sbjct: 205 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 264

Query: 276 PPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWA 335
           PPTSYLMIG GLR +P TNA++ISYTPLLINPLSPTFYYIAV  ITVDG KLPI+P VWA
Sbjct: 265 PPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPTVWA 324

Query: 336 IDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDLCVN---SSGR 395
           ID+QGNGGTVVDSGTTLTYL E AY EVLKAVR+RVKLP+ AELTPGFDLCVN    S R
Sbjct: 325 IDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAAELTPGFDLCVNVSKESQR 384

Query: 396 PSLPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEF 455
           PSLPR+RFR+G GAVFAPP RNYFLET E V+CLAIR V+ GNGFSVIGNLMQQGFLLEF
Sbjct: 385 PSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEF 444

Query: 456 DKDRSRLGFSRRGCGLP 470
           DK+ SRLGFSRRGCGLP
Sbjct: 445 DKEASRLGFSRRGCGLP 459

BLAST of Sed0009686 vs. NCBI nr
Match: XP_008467208.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo])

HSP 1 Score: 721.8 bits (1862), Expect = 3.7e-204
Identity = 361/474 (76.16%), Postives = 398/474 (83.97%), Query Frame = 0

Query: 1   MVYVSPFLTLI--IFALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTP 60
           M+ +SPF  LI   F  L    N NA  +A           A  ++LKLPLLH  P+S+P
Sbjct: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVA-----------AAADFLKLPLLHKPPFSSP 62

Query: 61  SQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVAD 120
           SQ+LSSDTHRLSLLF      R N T+KSPLISGASTGSGQYFV++RLGTPPQSLLLVAD
Sbjct: 63  SQSLSSDTHRLSLLF-----SRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVAD 122

Query: 121 TGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRR 180
           TGSDLVWVKCSAC NCSHHPPS+AF PRHSS+FSPFHC+ P CRL+PHAP  H CNHT  
Sbjct: 123 TGSDLVWVKCSACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHH-CNHTLL 182

Query: 181 HSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNG 240
           HSPC +LYSYADGSLSSGFFSKETTT  TLSGS+ H   LSFGCGFRISGPSVSGA+FNG
Sbjct: 183 HSPCRFLYSYADGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNG 242

Query: 241 ARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRI 300
           ARGVMGLGRG ISFSSQLGRRFG+ FSYCLMDYT+SPPPTS+LMIG GL  +P  NA++I
Sbjct: 243 ARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKI 302

Query: 301 SYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPEL 360
           SYTPL INPLSPTFYYI +N IT+DG KLPI+PAVW ID+QGNGGTVVDSGTTLTYL + 
Sbjct: 303 SYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKT 362

Query: 361 AYVEVLKAVRRRVKLPSLAELTPGFDLCVNSSG---RPSLPRLRFRLGGGAVFAPPPRNY 420
           AY EVLK+VRRRVKLP+ AELTPGFDLCVN+SG   RPSLPRLRFRLGGGAVFAPPPRNY
Sbjct: 363 AYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNY 422

Query: 421 FLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           FLETEE V+CLAIR V++GNGFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 423 FLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sed0009686 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.4e-57
Identity = 145/386 (37.56%), Postives = 202/386 (52.33%), Query Frame = 0

Query: 87  SPLISGASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPR 146
           S ++SG S GSG+YF  L +GTP + + +V DTGSD+VW++C+ C  C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 147 HSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFT 206
            S T++   C  P CR +     S  CN TRR + C Y  SY DGS + G FS ET TF 
Sbjct: 189 KSKTYATIPCSSPHCRRL----DSAGCN-TRRKT-CLYQVSYGDGSFTVGDFSTETLTF- 248

Query: 207 TLSGSQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSY 266
                +     ++ GCG    G       F GA G++GLG+G +SF  Q G RF   FSY
Sbjct: 249 ----RRNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 308

Query: 267 CLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEK 326
           CL+D + S  P+S +   A +  I         +TPLL NP   TFYY+ + GI+V G +
Sbjct: 309 CLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTR 368

Query: 327 LP-IDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDL 386
           +P +  +++ +D  GNGG ++DSGT++T L   AY+ +  A R   K    A     FD 
Sbjct: 369 VPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDT 428

Query: 387 CVNSSGRPSLP----RLRFRLGGGAVFAPPPRNYFLETEER-VVCLAIRPVDTGNGFSVI 446
           C + S    +      L FR   GA  + P  NY +  +     C A     T  G S+I
Sbjct: 429 CFDLSNMNEVKVPTVVLHFR---GADVSLPATNYLIPVDTNGKFCFAF--AGTMGGLSII 484

Query: 447 GNLMQQGFLLEFDKDRSRLGFSRRGC 467
           GN+ QQGF + +D   SR+GF+  GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Sed0009686 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.8e-55
Identity = 143/398 (35.93%), Postives = 206/398 (51.76%), Query Frame = 0

Query: 77  RRRRHNATIKSPLISGAST----GSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACL 136
           R R  NA ++S   SG  T    G G+Y +N+ +GTP  S   + DTGSDL+W +C  C 
Sbjct: 71  RMRSINAMLQSS--SGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCT 130

Query: 137 NCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGS 196
            C    P+  F P+ SS+FS   C    C+ +P    S TCN    ++ C Y Y Y DGS
Sbjct: 131 QC-FSQPTPIFNPQDSSSFSTLPCESQYCQDLP----SETCN----NNECQYTYGYGDGS 190

Query: 197 LSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISF 256
            + G+ + ET TF T S       +++FGCG    G      + NGA G++G+G GP+S 
Sbjct: 191 TTQGYMATETFTFETSS-----VPNIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSL 250

Query: 257 SSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTF 316
            SQLG      FSYC+  Y  S P T  L +G+    +P  + S    T L+ + L+PT+
Sbjct: 251 PSQLG---VGQFSYCMTSYGSSSPST--LALGSAASGVPEGSPS----TTLIHSSLNPTY 310

Query: 317 YYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRRRVK 376
           YYI + GITV G+ L I  + + + D G GG ++DSGTTLTYLP+ AY  V +A   ++ 
Sbjct: 311 YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN 370

Query: 377 LPSLAELTPGFDLCVNSSGRPS---LPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIR 436
           LP++ E + G   C       S   +P +  +  GG V     +N  +   E V+CLA+ 
Sbjct: 371 LPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMG 430

Query: 437 PVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCG 468
              +  G S+ GN+ QQ   + +D     + F    CG
Sbjct: 431 S-SSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCG 436

BLAST of Sed0009686 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.5e-54
Identity = 143/401 (35.66%), Postives = 209/401 (52.12%), Query Frame = 0

Query: 75  SHRRRRHNATIKSPLISGAST----GSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSA 134
           S R +R  A +  P  SG  T    G G+Y +NL +GTP Q    + DTGSDL+W +C  
Sbjct: 68  SRRLQRLEAMLNGP--SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQP 127

Query: 135 CLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYAD 194
           C  C  +  +  F P+ SS+FS   C    C+    A SS TC+    ++ C Y Y Y D
Sbjct: 128 CTQC-FNQSTPIFNPQGSSSFSTLPCSSQLCQ----ALSSPTCS----NNFCQYTYGYGD 187

Query: 195 GSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPI 254
           GS + G    ET TF ++S       +++FGCG    G      + NGA G++G+GRGP+
Sbjct: 188 GSETQGSMGTETLTFGSVS-----IPNITFGCGENNQG----FGQGNGA-GLVGMGRGPL 247

Query: 255 SFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSP 314
           S  SQL       FSYC+     S P  S L++G+    + A + +    T L+ +   P
Sbjct: 248 SLPSQLDVT---KFSYCMTPIGSSTP--SNLLLGSLANSVTAGSPN----TTLIQSSQIP 307

Query: 315 TFYYIAVNGITVDGEKLPIDPAVWAID-DQGNGGTVVDSGTTLTYLPELAYVEVLKAVRR 374
           TFYYI +NG++V   +LPIDP+ +A++ + G GG ++DSGTTLTY    AY  V +    
Sbjct: 308 TFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFIS 367

Query: 375 RVKLPSLAELTPGFDLCVNSSGRPS---LPRLRFRLGGGAVFAPPPRNYFLETEERVVCL 434
           ++ LP +   + GFDLC  +   PS   +P       GG +   P  NYF+     ++CL
Sbjct: 368 QINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFISPSNGLICL 427

Query: 435 AIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCG 468
           A+    +  G S+ GN+ QQ  L+ +D   S + F+   CG
Sbjct: 428 AMG--SSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

BLAST of Sed0009686 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.6e-48
Identity = 128/400 (32.00%), Postives = 199/400 (49.75%), Query Frame = 0

Query: 74  YSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLN 133
           Y+   R     + +P++SGAS GSG+YF  + +GTP + + LV DTGSD+ W++C  C +
Sbjct: 136 YNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCAD 195

Query: 134 CSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSL 193
           C +      F P  SST+    C  PQC L+     +  C    R + C Y  SY DGS 
Sbjct: 196 C-YQQSDPVFNPTSSSTYKSLTCSAPQCSLL----ETSAC----RSNKCLYQVSYGDGSF 255

Query: 194 SSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFS 253
           + G  + +T TF    G+     +++ GCG    G       F GA G++GLG G +S +
Sbjct: 256 TVGELATDTVTF----GNSGKINNVALGCGHDNEG------LFTGAAGLLGLGGGVLSIT 315

Query: 254 SQLGRRFGHTFSYCLMDYTISPPPT---SYLMIGAGLRHIPATNASRISYTPLLINPLSP 313
           +Q+      +FSYCL+D       +   + + +G G     AT        PLL N    
Sbjct: 316 NQMK---ATSFSYCLVDRDSGKSSSLDFNSVQLGGG----DAT-------APLLRNKKID 375

Query: 314 TFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLKA-VRR 373
           TFYY+ ++G +V GEK+ +  A++ +D  G+GG ++D GT +T L   AY  +  A ++ 
Sbjct: 376 TFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKL 435

Query: 374 RVKLPSLAELTPGFDLCVNSSGRPS--LPRLRFRLGGGAVFAPPPRNYFLETEER-VVCL 433
            V L   +     FD C + S   +  +P + F   GG     P +NY +  ++    C 
Sbjct: 436 TVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCF 495

Query: 434 AIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGC 467
           A  P  T +  S+IGN+ QQG  + +D  ++ +G S   C
Sbjct: 496 AFAP--TSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Sed0009686 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 7.8e-48
Identity = 147/447 (32.89%), Postives = 206/447 (46.09%), Query Frame = 0

Query: 32  ADVDVDADAEVEYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLIS 91
           AD   D    ++      L   P S     + +D  R SL+    R+R     +K  L S
Sbjct: 41  ADSMKDTSVRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLI---SRKRNSTVGVKMDLGS 100

Query: 92  GASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTF 151
           G   G+ QYF  +R+GTP +   +V DTGS+L WV C              F    S +F
Sbjct: 101 GIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCR--YRARGKDNRRVFRADESKSF 160

Query: 152 SPFHCYHPQCRL-VPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSG 211
               C    C++ + +  S  TC      +PC Y Y YADGS + G F+KET T    +G
Sbjct: 161 KTVGCLTQTCKVDLMNLFSLTTC--PTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNG 220

Query: 212 SQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMD 271
                     GC       S +G  F GA GV+GL     SF+S     +G  FSYCL+D
Sbjct: 221 RMARLPGHLIGC-----SSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVD 280

Query: 272 YTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPID 331
           +  +   ++YL+ G+      +T  +    TPL +  + P FY I V GI++  + L I 
Sbjct: 281 HLSNKNVSNYLIFGSS----RSTKTAFRRTTPLDLTRI-PPFYAINVIGISLGYDMLDIP 340

Query: 332 PAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRR------RVK-----LPSLAEL 391
             VW  D    GGT++DSGT+LT L + AY +V+  + R      RVK     +      
Sbjct: 341 SQVW--DATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSF 400

Query: 392 TPGFDLCVNSSGRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSV 451
           T GF++         LP+L F L GGA F P  ++Y ++    V CL      T    +V
Sbjct: 401 TSGFNV-------SKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGT-PATNV 460

Query: 452 IGNLMQQGFLLEFDKDRSRLGFSRRGC 467
           IGN+MQQ +L EFD   S L F+   C
Sbjct: 461 IGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of Sed0009686 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.1e-205
Identity = 361/471 (76.65%), Postives = 400/471 (84.93%), Query Frame = 0

Query: 4   VSPFLTLIIF--ALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTPSQT 63
           +SPF  LI+     L   PN NA  +A           A  ++LKLPLLH  P+S+PSQ+
Sbjct: 6   ISPFFLLILLFSFFLTHLPNPNATAVA-----------APADFLKLPLLHKPPFSSPSQS 65

Query: 64  LSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTGS 123
           LSSDTHRLSLLF      R N T+KSPLISGASTGSGQYFV++RLGTPPQSLLLVADTGS
Sbjct: 66  LSSDTHRLSLLF-----SRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGS 125

Query: 124 DLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSP 183
           DLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFHC+ P CRL+PHAP  H CNHTR HSP
Sbjct: 126 DLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP-HHLCNHTRLHSP 185

Query: 184 CHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGARG 243
           C +LYSYADGSLSSGFFSKETTT  +LSGS+ H   LSFGCGFRISGPSVSGA+FNGARG
Sbjct: 186 CRFLYSYADGSLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARG 245

Query: 244 VMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYT 303
           VMGLGRG ISFSSQLGRRFG+ FSYCLMDYT+SPPPTS+LMIG GL  +P TNA++ISYT
Sbjct: 246 VMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYT 305

Query: 304 PLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYV 363
           PL INPLSPTFYYI ++ IT+DG KLPI+PAVW ID+QGNGGTVVDSGTTLTYL + AY 
Sbjct: 306 PLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYE 365

Query: 364 EVLKAVRRRVKLPSLAELTPGFDLCVNSSG---RPSLPRLRFRLGGGAVFAPPPRNYFLE 423
           EVLK+VRRRVKLP+ AELTPGFDLCVN+SG   RPSLPRLRFRLGGGAVFAPPPRNYFLE
Sbjct: 366 EVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLE 425

Query: 424 TEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           TEE V+CLAIR V++GNGFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 426 TEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sed0009686 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.1e-205
Identity = 357/437 (81.69%), Postives = 388/437 (88.79%), Query Frame = 0

Query: 36  VDADAEVEYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGAST 95
           ++A A  +YLKLPLLH NP+S+PSQ LSSDTHRLSLLF S  RRR N T+KSPLISGAST
Sbjct: 25  LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF-SALRRRPNPTLKSPLISGAST 84

Query: 96  GSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFH 155
           GSGQYFV+LR+GTPPQSLLLVADTGSDLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFH
Sbjct: 85  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 144

Query: 156 CYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHT 215
           C+ P CRL+PHAP  H CNHTR HSPC +LY+YADGS SSGFFSKETTT  TLSGS+T  
Sbjct: 145 CFDPHCRLLPHAP-PHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRL 204

Query: 216 ASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISP 275
             LSFGCGFRISGPSVSGA+FNGARGVMGLGRGPISFS+QLGRRFG+ FSYCLMDYT+SP
Sbjct: 205 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 264

Query: 276 PPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWA 335
           PPTSYLMIG GLR +P TNA++ISYTPLLINPLSPTFYYIAV  ITVDG KLPI+P VWA
Sbjct: 265 PPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPTVWA 324

Query: 336 IDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDLCVN---SSGR 395
           ID+QGNGGTVVDSGTTLTYL E AY EVLKAVR+RVKLP+ AELTPGFDLCVN    S R
Sbjct: 325 IDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAAELTPGFDLCVNVSKESQR 384

Query: 396 PSLPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEF 455
           PSLPR+RFR+G GAVFAPP RNYFLET E V+CLAIR V+ GNGFSVIGNLMQQGFLLEF
Sbjct: 385 PSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEF 444

Query: 456 DKDRSRLGFSRRGCGLP 470
           DK+ SRLGFSRRGCGLP
Sbjct: 445 DKEASRLGFSRRGCGLP 459

BLAST of Sed0009686 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 361/474 (76.16%), Postives = 398/474 (83.97%), Query Frame = 0

Query: 1   MVYVSPFLTLI--IFALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTP 60
           M+ +SPF  LI   F  L    N NA  +A           A  ++LKLPLLH  P+S+P
Sbjct: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVA-----------AAADFLKLPLLHKPPFSSP 62

Query: 61  SQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVAD 120
           SQ+LSSDTHRLSLLF      R N T+KSPLISGASTGSGQYFV++RLGTPPQSLLLVAD
Sbjct: 63  SQSLSSDTHRLSLLF-----SRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVAD 122

Query: 121 TGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRR 180
           TGSDLVWVKCSAC NCSHHPPS+AF PRHSS+FSPFHC+ P CRL+PHAP  H CNHT  
Sbjct: 123 TGSDLVWVKCSACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHH-CNHTLL 182

Query: 181 HSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNG 240
           HSPC +LYSYADGSLSSGFFSKETTT  TLSGS+ H   LSFGCGFRISGPSVSGA+FNG
Sbjct: 183 HSPCRFLYSYADGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNG 242

Query: 241 ARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRI 300
           ARGVMGLGRG ISFSSQLGRRFG+ FSYCLMDYT+SPPPTS+LMIG GL  +P  NA++I
Sbjct: 243 ARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKI 302

Query: 301 SYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPEL 360
           SYTPL INPLSPTFYYI +N IT+DG KLPI+PAVW ID+QGNGGTVVDSGTTLTYL + 
Sbjct: 303 SYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKT 362

Query: 361 AYVEVLKAVRRRVKLPSLAELTPGFDLCVNSSG---RPSLPRLRFRLGGGAVFAPPPRNY 420
           AY EVLK+VRRRVKLP+ AELTPGFDLCVN+SG   RPSLPRLRFRLGGGAVFAPPPRNY
Sbjct: 363 AYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNY 422

Query: 421 FLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           FLETEE V+CLAIR V++GNGFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 423 FLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sed0009686 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 3.0e-204
Identity = 364/472 (77.12%), Postives = 401/472 (84.96%), Query Frame = 0

Query: 1   MVYVSPFLTLIIFALLLRSPNANANDIADADADVDVDADAEVEYLKLPLLHDNPYSTPSQ 60
           M+ VSPF  LI+    L              AD+ ++A A  +YLKLPLLH NP+S+PSQ
Sbjct: 5   MLSVSPFFHLILLLFSL--------------ADL-LNAAAAADYLKLPLLHKNPFSSPSQ 64

Query: 61  TLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFVNLRLGTPPQSLLLVADTG 120
            LSSDTHRLSLLF S  RRR N T+KSPLISGASTGSGQYFV+LR+GTPPQSLLLVADTG
Sbjct: 65  ALSSDTHRLSLLF-SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTG 124

Query: 121 SDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHS 180
           SDLVWVKCSAC NCSHHPPS+AFLPRHSS+FSPFHC+ P CRL+PHAP  H CNHTR HS
Sbjct: 125 SDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP-PHLCNHTRLHS 184

Query: 181 PCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGCGFRISGPSVSGARFNGAR 240
           PC +LY+YADGS SSGFFSKETTT  TL+GS+T    LSFGCGFRISGPSVSGA+FNGAR
Sbjct: 185 PCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGAR 244

Query: 241 GVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISY 300
           GVMGLGRGPISFS+QLGRRFG+ FSYCLMDYT+SPPPTSYLMIG GLR +P TNA++ISY
Sbjct: 245 GVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISY 304

Query: 301 TPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAY 360
           TPLLINPLSPTFYYIAV  ITVDG KLPI+P +WAID+QGNGGTVVDSGTTLTYL E AY
Sbjct: 305 TPLLINPLSPTFYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAY 364

Query: 361 VEVLKAVRRRVKLPSLAELTPGFDLCV---NSSGRPSLPRLRFRLGGGAVFAPPPRNYFL 420
            EVLKA+R+RVKLP+ AELTPGFDLCV   N S RPSLPR+RF+LG GAVF PP RNYFL
Sbjct: 365 KEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFL 424

Query: 421 ETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSRLGFSRRGCGLP 470
           ETEE V+CLAIR V+ GNGFSVIGNLMQQGFLLEFDK+ SRLGFSRRGCGLP
Sbjct: 425 ETEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sed0009686 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 5.0e-199
Identity = 342/432 (79.17%), Postives = 375/432 (86.81%), Query Frame = 0

Query: 43  EYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFV 102
           +YLK PLLH NP+S+PSQ LSSDTHRLSLLF +H   RH+ T+KSPLISGASTGSGQYFV
Sbjct: 28  QYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSAH---RHSPTLKSPLISGASTGSGQYFV 87

Query: 103 NLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCR 162
           NL LGTPPQSLLLV DTGSDLVWVKCS C NCSHHPPS+AF PRHSS+FSPFHC+ P CR
Sbjct: 88  NLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCR 147

Query: 163 LVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTA--SLSF 222
           L+PH P SH CNHT  HSPC +LYSYAD SLSSGFFSK+ TTF T SG+ T T    LSF
Sbjct: 148 LLPH-PPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSF 207

Query: 223 GCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSY 282
           GCGFRISGPSVSGARF GARGVMGLGRGPISFSSQLG RFG+TFSYCLMDYT+SPPPTSY
Sbjct: 208 GCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGHRFGNTFSYCLMDYTLSPPPTSY 267

Query: 283 LMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQG 342
           LMIG GLR +P TNAS+ISYTPL INPLSPTFYYI V  ITVDG KLPI+P VWAID+QG
Sbjct: 268 LMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPKVWAIDEQG 327

Query: 343 NGGTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDLCVNSSGRP---SLPR 402
           NGGTVVDSGTTLTYL E AY EVLKA+RRRVKLP   +L+PGFDLCVN+S      SLP+
Sbjct: 328 NGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASSESRMRSLPQ 387

Query: 403 LRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRS 462
           +RFR+GGG VFAPP RNYF+ETEE V+CLAIRPVD+GNGFSVIGNLMQQGFLLEFD+++S
Sbjct: 388 IRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDREKS 447

Query: 463 RLGFSRRGCGLP 470
           R+GFSRRGCGLP
Sbjct: 448 RMGFSRRGCGLP 455

BLAST of Sed0009686 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 587.8 bits (1514), Expect = 7.7e-168
Identity = 289/431 (67.05%), Postives = 335/431 (77.73%), Query Frame = 0

Query: 43  EYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFV 102
           +YLKLPLL  +P+ +P+Q L+ DT RL  L     RR+    +KSP++SGA++GSGQYFV
Sbjct: 30  KYLKLPLLRKSPFPSPTQALALDTRRLHFL---SLRRKPIPFVKSPVVSGAASGSGQYFV 89

Query: 103 NLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCR 162
           +LR+G PPQSLLL+ADTGSDLVWVKCSAC NCSHH P+T F PRHSSTFSP HCY P CR
Sbjct: 90  DLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSPATVFFPRHSSTFSPAHCYDPVCR 149

Query: 163 LVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGC 222
           LVP    +  CNHTR HS CHY Y YADGSL+SG F++ETT+  T SG +    S++FGC
Sbjct: 150 LVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGC 209

Query: 223 GFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLM 282
           GFRISG SVSG  FNGA GVMGLGRGPISF+SQLGRRFG+ FSYCLMDYT+SPPPTSYL+
Sbjct: 210 GFRISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNKFSYCLMDYTLSPPPTSYLI 269

Query: 283 IGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNG 342
           IG G         S++ +TPLL NPLSPTFYY+ +  + V+G KL IDP++W IDD GNG
Sbjct: 270 IGNG-----GDGISKLFFTPLLTNPLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNG 329

Query: 343 GTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDLCVNSSG--RPS--LPRL 402
           GTVVDSGTTL +L E AY  V+ AVRRRVKLP    LTPGFDLCVN SG  +P   LPRL
Sbjct: 330 GTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRL 389

Query: 403 RFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSR 462
           +F   GGAVF PPPRNYF+ETEE++ CLAI+ VD   GFSVIGNLMQQGFL EFD+DRSR
Sbjct: 390 KFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSR 449

Query: 463 LGFSRRGCGLP 470
           LGFSRRGC LP
Sbjct: 450 LGFSRRGCALP 452

BLAST of Sed0009686 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.9e-110
Identity = 219/431 (50.81%), Postives = 252/431 (58.47%), Query Frame = 0

Query: 43  EYLKLPLLHDNPYSTPSQTLSSDTHRLSLLFYSHRRRRHNATIKSPLISGASTGSGQYFV 102
           +YLKLPLL  +P+ +P+Q L+ DT RL  L     RR+    +KSP++SGA++GSGQYFV
Sbjct: 30  KYLKLPLLRKSPFPSPTQALALDTRRLHFL---SLRRKPIPFVKSPVVSGAASGSGQYFV 89

Query: 103 NLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHSSTFSPFHCYHPQCR 162
           +LR+G PPQSLLL+ADTGSDLVWVKCSAC NCSHH P+T F PRHSSTFSP HCY P CR
Sbjct: 90  DLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSPATVFFPRHSSTFSPAHCYDPVCR 149

Query: 163 LVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFTTLSGSQTHTASLSFGC 222
           LVP    +  CNHTR HS CHY Y YADGSL+SG F++ETT+  T SG +    S++FGC
Sbjct: 150 LVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGC 209

Query: 223 GFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSYCLMDYTISPPPTSYLM 282
           GFRISG SVS                                                  
Sbjct: 210 GFRISGQSVS-------------------------------------------------- 269

Query: 283 IGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEKLPIDPAVWAIDDQGNG 342
                                                                    GNG
Sbjct: 270 ---------------------------------------------------------GNG 329

Query: 343 GTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDLCVNSSG--RPS--LPRL 402
           GTVVDSGTTL +L E AY  V+ AVRRRVKLP    LTPGFDLCVN SG  +P   LPRL
Sbjct: 330 GTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRL 350

Query: 403 RFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSVIGNLMQQGFLLEFDKDRSR 462
           +F   GGAVF PPPRNYF+ETEE++ CLAI+ VD   GFSVIGNLMQQGFL EFD+DRSR
Sbjct: 390 KFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSR 350

Query: 463 LGFSRRGCGLP 470
           LGFSRRGC LP
Sbjct: 450 LGFSRRGCALP 350

BLAST of Sed0009686 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 229.9 bits (585), Expect = 4.1e-60
Identity = 149/389 (38.30%), Postives = 205/389 (52.70%), Query Frame = 0

Query: 89  LISGASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHS 148
           L SG + GSG+YF+++ +GTPP+   L+ DTGSDL W++C  C +C  H     + P+ S
Sbjct: 149 LESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYDPKTS 208

Query: 149 STFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTT--FT 208
           ++F    C  P+C L+        C     +  C Y Y Y D S ++G F+ ET T   T
Sbjct: 209 ASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETFTVNLT 268

Query: 209 TLSG--SQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTF 268
           T  G  S+    ++ FGCG    G       F+GA G++GLGRGP+SFSSQL   +GH+F
Sbjct: 269 TTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLYGHSF 328

Query: 269 SYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDG 328
           SYCL+D   +   +S L+ G     +  TN +  S+     N +  TFYYI +  I V G
Sbjct: 329 SYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKSILVGG 388

Query: 329 EKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLK---AVRRRVKLPSLAELTP 388
           + L I    W I   G+GGT++DSGTTL+Y  E AY E++K   A + +   P   +  P
Sbjct: 389 KALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAY-EIIKNKFAEKMKENYPIFRDF-P 448

Query: 389 GFDLCVNSSG----RPSLPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGF 448
             D C N SG       LP L      G V+  P  N F+   E +VCLAI        F
Sbjct: 449 VLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST-F 508

Query: 449 SVIGNLMQQGFLLEFDKDRSRLGFSRRGC 467
           S+IGN  QQ F + +D  RSRLGF+   C
Sbjct: 509 SIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of Sed0009686 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.7e-58
Identity = 145/386 (37.56%), Postives = 202/386 (52.33%), Query Frame = 0

Query: 87  SPLISGASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPR 146
           S ++SG S GSG+YF  L +GTP + + +V DTGSD+VW++C+ C  C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 147 HSSTFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTTFT 206
            S T++   C  P CR +     S  CN TRR + C Y  SY DGS + G FS ET TF 
Sbjct: 189 KSKTYATIPCSSPHCRRL----DSAGCN-TRRKT-CLYQVSYGDGSFTVGDFSTETLTF- 248

Query: 207 TLSGSQTHTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTFSY 266
                +     ++ GCG    G       F GA G++GLG+G +SF  Q G RF   FSY
Sbjct: 249 ----RRNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 308

Query: 267 CLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDGEK 326
           CL+D + S  P+S +   A +  I         +TPLL NP   TFYY+ + GI+V G +
Sbjct: 309 CLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTR 368

Query: 327 LP-IDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLKAVRRRVKLPSLAELTPGFDL 386
           +P +  +++ +D  GNGG ++DSGT++T L   AY+ +  A R   K    A     FD 
Sbjct: 369 VPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDT 428

Query: 387 CVNSSGRPSLP----RLRFRLGGGAVFAPPPRNYFLETEER-VVCLAIRPVDTGNGFSVI 446
           C + S    +      L FR   GA  + P  NY +  +     C A     T  G S+I
Sbjct: 429 CFDLSNMNEVKVPTVVLHFR---GADVSLPATNYLIPVDTNGKFCFAF--AGTMGGLSII 484

Query: 447 GNLMQQGFLLEFDKDRSRLGFSRRGC 467
           GN+ QQGF + +D   SR+GF+  GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Sed0009686 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-57
Identity = 146/387 (37.73%), Postives = 202/387 (52.20%), Query Frame = 0

Query: 89  LISGASTGSGQYFVNLRLGTPPQSLLLVADTGSDLVWVKCSACLNCSHHPPSTAFLPRHS 148
           L SG + GSG+YF+++ +G+PP+   L+ DTGSDL W++C  C +C        + P+ S
Sbjct: 159 LESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDC-FQQNGAFYDPKAS 218

Query: 149 STFSPFHCYHPQCRLVPHAPSSHTCNHTRRHSPCHYLYSYADGSLSSGFFSKETTT--FT 208
           +++    C   +C LV        C     +  C Y Y Y D S ++G F+ ET T   T
Sbjct: 219 ASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETFTVNLT 278

Query: 209 TLSGSQT--HTASLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGHTF 268
           T  GS    +  ++ FGCG    G       F+GA G++GLGRGP+SFSSQL   +GH+F
Sbjct: 279 TNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSLYGHSF 338

Query: 269 SYCLMDYTISPPPTSYLMIGAGLRHIPATNASRISYTPLLINPLSPTFYYIAVNGITVDG 328
           SYCL+D       +S L+ G     +   N +  S+     N L  TFYY+ +  I V G
Sbjct: 339 SYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKSILVAG 398

Query: 329 EKLPIDPAVWAIDDQGNGGTVVDSGTTLTYLPELAYVEVLK---AVRRRVKLPSLAELTP 388
           E L I    W I   G GGT++DSGTTL+Y  E AY E +K   A + + K P   +  P
Sbjct: 399 EVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAY-EFIKNKIAEKAKGKYPVYRDF-P 458

Query: 389 GFDLCVNSSG--RPSLPRLRFRLGGGAVFAPPPRNYFLETEERVVCLAIRPVDTGNGFSV 448
             D C N SG     LP L      GAV+  P  N F+   E +VCLA+      + FS+
Sbjct: 459 ILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPK-SAFSI 518

Query: 449 IGNLMQQGFLLEFDKDRSRLGFSRRGC 467
           IGN  QQ F + +D  RSRLG++   C
Sbjct: 519 IGNYQQQNFHILYDTKRSRLGYAPTKC 532