Sed0009438 (gene) Chayote v1

BLAST of Sed0009438 vs. NCBI nr
Match: XP_038894997.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A isoform X1 [Benincasa hispida])

HSP 1 Score: 1217.2 bits (3148), Expect = 0.0e+00
Identity = 618/682 (90.62%), Postives = 646/682 (94.72%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQLQ R+LQLIFLI+ LCS+ I ARPFLIVISQDDLKD A  DDS+ + +SDSAEWDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLCSIFINARPFLIVISQDDLKDGAPPDDSSDSANSDSAEWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVA 120
           EPES N ALELDPG+WRPIFEPDS   ASDAP D YYTALGK++SAVSSGD+RLMEDAVA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDS--PASDAPDDLYYTALGKMMSAVSSGDLRLMEDAVA 120

Query: 121 DVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYFRQ 180
           D+D+ AAESGDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YFRQ
Sbjct: 121 DIDQAAAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYFRQ 180

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 240

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300

Query: 301 GVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEESGG 360
           GVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA NEESGG
Sbjct: 301 GVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENEESGG 360

Query: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATAL 420
           HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+AL
Sbjct: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 420

Query: 421 YKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSI 480
           YKLVAERGPWSSLSRWALESYLK+DIGKAF LYARMAELGYEVAQSNAAWILDKYGEQS+
Sbjct: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFLLYARMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 481 CLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540
           CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 481 CLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540

Query: 541 KSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNH 600
           KSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR NH
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRKNH 600

Query: 601 ADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVR- 660
           ADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRR AAVR 
Sbjct: 601 ADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAAVRA 660

Query: 661 ----AAAQAVPLQPNDHGVPQN 677
               AAA+AVPL PNDH  PQN
Sbjct: 661 AAAAAAAEAVPLHPNDHVPPQN 680

BLAST of Sed0009438 vs. NCBI nr
Match: XP_004137505.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A [Cucumis sativus] >KGN64178.1 hypothetical protein Csa_014040 [Cucumis sativus])

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 617/679 (90.87%), Postives = 647/679 (95.29%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQLQ R+LQLIFLI+ L SL I ARPFLIVISQDDLKD A  DDS+ + +SDSA+WDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAA--SDAPPDPYYTALGKIISAVSSGDVRLMEDA 120
           EPES N ALELDPG+WRPIFEPDSTA+A  SDAP D YYTALGK++SAVSSGD+RLMEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSTASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYF 180
           VAD+D+  AESGDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YF
Sbjct: 121 VADIDQAVAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEES 360
           GAGVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAT 420
           GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540
           S+CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRN 600
           HAKSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRM 600

Query: 601 NHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660
           NHADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRR AAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAAV 660

Query: 661 RAAAQAVPLQPNDHGVPQN 677
           R AA+AVPL PNDH  PQN
Sbjct: 661 R-AAEAVPLHPNDHVPPQN 678

BLAST of Sed0009438 vs. NCBI nr
Match: XP_008464831.1 (PREDICTED: ERAD-associated E3 ubiquitin-protein ligase component HRD3A [Cucumis melo] >TYJ99036.1 ERAD-associated E3 ubiquitin-protein ligase component HRD3A [Cucumis melo var. makuwa])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 613/683 (89.75%), Postives = 646/683 (94.58%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQL+ R++QLIFLI+ L SL I ARPFLIVISQDDLKD A  DDS+ + +SDSA+WDEFG
Sbjct: 1   MQLETRRVQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAA--SDAPPDPYYTALGKIISAVSSGDVRLMEDA 120
           EPES N ALELDPG+WRPIFEPDS A+A  SDAP D YYTALGK++SAVSSGD+RLMEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSLASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYF 180
           VAD+D+  A++GDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YF
Sbjct: 121 VADIDQAVADNGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEES 360
           GAGVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAT 420
           GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540
           S+CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRN 600
           HAKSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRK 600

Query: 601 NHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660
           NHADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRRQAAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660

Query: 661 RAAAQAV----PLQPNDHGVPQN 677
           RAAA A     PL PNDH  PQN
Sbjct: 661 RAAAAAAEAVPPLHPNDHLPPQN 683

BLAST of Sed0009438 vs. NCBI nr
Match: XP_022924200.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata] >XP_022924201.1 ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 608/679 (89.54%), Postives = 641/679 (94.40%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSS-DSAEWDEFG 60
           MQLQ R++QLIFLII LCS  I ARPFLIVISQDDLKDAA  DDS+  ++ DSAEWDEFG
Sbjct: 5   MQLQARRIQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 64

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVA 120
           EPES N A ELDPGTWRPIFEPDS A+  DAP D YYTALGK+++AVSSGD+RLMEDAV 
Sbjct: 65  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 124

Query: 121 DVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYFRQ 180
           D+D++A ESGDPHAQS+LGLLYG+GIMKE NK KAFMYH+F+AEGNKQSKMA+AY+YFRQ
Sbjct: 125 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 184

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQIL
Sbjct: 185 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 244

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEPKSMELLGEIYARGA
Sbjct: 245 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 304

Query: 301 GVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEESGG 360
           GVERDYTKA QWLT+ASKHP+FSAYNG+GYLYVKGYGV+KNFTKAKEYFEKAANN+ESGG
Sbjct: 305 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 364

Query: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATAL 420
           HYNLGVMYLKGIGVKRDVKKACTHFI+AANAGQPKAFYQLAKMFHTGVGLKRNIPMA+AL
Sbjct: 365 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 424

Query: 421 YKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSI 480
           YKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQS+
Sbjct: 425 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 484

Query: 481 CLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540
           CLGESGFC+DAE HQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 485 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 544

Query: 541 KSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNH 600
           KSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLR NH
Sbjct: 545 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 604

Query: 601 ADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVR- 660
           ADSFLVHVIDSLPEVYPKID WVE+VLLEEGNATILTLFACLLTVLYLRERQRR A VR 
Sbjct: 605 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 664

Query: 661 -AAAQAVPLQPNDHGVPQN 677
            AAA+AVPL PNDH   QN
Sbjct: 665 AAAAEAVPLHPNDHVAAQN 683

BLAST of Sed0009438 vs. NCBI nr
Match: KAG7019908.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 609/682 (89.30%), Postives = 641/682 (93.99%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSS-DSAEWDEFG 60
           MQLQ R+LQLIFLII LCS  I ARPFLIVISQDDLKDAA  DDS+  ++ DSAEWDEFG
Sbjct: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVA 120
           EPES N A ELDPGTWRPIFEPDS A+  DAP D YYTALGK+++AVSSGD+RLMEDAV 
Sbjct: 96  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 155

Query: 121 DVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYFRQ 180
           D+D++A ESGDPHAQS+LGLLYG+GIMKE NK KAFMYH+F+AEGNKQSKMA+AY+YFRQ
Sbjct: 156 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 215

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQIL
Sbjct: 216 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 275

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEPKSMELLGEIYARGA
Sbjct: 276 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 335

Query: 301 GVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEESGG 360
           GVERDYTKA QWLT+ASKHP+FSAYNG+GYLYVKGYGV+KNFTKAKEYFEKAANN+ESGG
Sbjct: 336 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 395

Query: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATAL 420
           HYNLGVMYLKGIGVKRDVKKACTHFI+AANAGQPKAFYQLAKMFHTGVGLKRNIPMA+AL
Sbjct: 396 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 455

Query: 421 YKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSI 480
           YKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQS+
Sbjct: 456 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 515

Query: 481 CLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540
           CLGESGFC+DAE HQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 516 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 575

Query: 541 KSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNH 600
           KSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLR NH
Sbjct: 576 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 635

Query: 601 ADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVR- 660
           ADSFLVHVIDSLPEVYPKID WVE+VLLEEGNATILTLFACLLTVLYLRERQRR A VR 
Sbjct: 636 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 695

Query: 661 ----AAAQAVPLQPNDHGVPQN 677
               AAA+AVPL PNDH   QN
Sbjct: 696 AAAAAAAEAVPLHPNDHVAAQN 717

BLAST of Sed0009438 vs. ExPASy Swiss-Prot
Match: Q9LM25 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Arabidopsis thaliana OX=3702 GN=HRD3A PE=1 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 1.5e-270
Identity = 467/670 (69.70%), Postives = 560/670 (83.58%), Query Frame = 0

Query: 10  LIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSSDSAEWDEFGEPESHNPALE 69
           L  L+ S     + ARP ++V+S DDL      DD+     +S+++DEFGE E  +   E
Sbjct: 11  LSLLVFSFIEFGVHARPVVLVLSNDDLNSG--GDDNGV--GESSDFDEFGESEPKSEE-E 70

Query: 70  LDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVADVDRLAAESG 129
           LDPG+WR IFEPD +   + +P   YY+ L KI+SA S G+ RLME+AV +++  A+ +G
Sbjct: 71  LDPGSWRSIFEPDDSTVQAASP--QYYSGLKKILSAASEGNFRLMEEAVDEIE-AASSAG 130

Query: 130 DPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAE-GNKQSKMAVAYTYFRQEMYEKAVKL 189
           DPHAQS++G +YG+G+M+E +K K+F++H+F+A  GN QSKMA+A+TY RQ+M++KAV+L
Sbjct: 131 DPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDKAVQL 190

Query: 190 YAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKGNA 249
           YAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEED+DFQILEYQAQKGNA
Sbjct: 191 YAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQKGNA 250

Query: 250 GAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGAGVERDYTKA 309
            AMY+IGLFYYFGLRGLRRDH KAL WF KAV+KGEP+SMELLGEIYARGAGVER+YTKA
Sbjct: 251 NAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGAGVERNYTKA 310

Query: 310 FQWLTQASKHPAFSAYNGMGYLYVKGYGVD-KNFTKAKEYFEKAANNEESGGHYNLGVMY 369
            +WLT A+K   +SA+NG+GYLYVKGYGVD KN+TKA+EYFEKA +NE+  GHYNLGV+Y
Sbjct: 311 LEWLTLAAKEGLYSAFNGIGYLYVKGYGVDKKNYTKAREYFEKAVDNEDPSGHYNLGVLY 370

Query: 370 LKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAERG 429
           LKGIGV RDV++A  +F +AANAGQPKAFYQLAKMFHTGVGLK+N+ MAT+ YKLVAERG
Sbjct: 371 LKGIGVNRDVRQATKYFFVAANAGQPKAFYQLAKMFHTGVGLKKNLEMATSFYKLVAERG 430

Query: 430 PWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSICLGESGFC 489
           PWSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+S+C+G SGFC
Sbjct: 431 PWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGVSGFC 490

Query: 490 SDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQA 549
           +D ERH+RAHSLWW ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYMHAKSQ NAQA
Sbjct: 491 TDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQSNAQA 550

Query: 550 MFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNHADSFLVHV 609
           MFNLGYMHEHGQGLPFDLHLAKRYYD++L+ D AARLPV LAL SLWLR N+AD+ LV V
Sbjct: 551 MFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTVLVRV 610

Query: 610 IDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVRAAAQAVPL- 669
           +DSLPEVYPK++ W+E V+ EEGNATILTLF CL+T+LYLRERQRRQ  V A   A  + 
Sbjct: 611 VDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRERQRRQVVVVADPVAADVA 670

Query: 670 QPNDHGVPQN 677
           QP D  V Q+
Sbjct: 671 QPLDADVAQH 672

BLAST of Sed0009438 vs. ExPASy Swiss-Prot
Match: Q10NT7 (ERAD-associated E3 ubiquitin-protein ligase component HRD3 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD3 PE=2 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 2.4e-244
Identity = 435/683 (63.69%), Postives = 537/683 (78.62%), Query Frame = 0

Query: 2   QLQMRKLQLIFLIISLCSLSIL-----ARPFLIVISQDD-LKDAALADDSAADS-SDSAE 61
           +++ R L L+   ++  + ++L      RPF++V+S+DD LKD A A  S   + +DS E
Sbjct: 17  RVRHRHLLLLVAAVAAAASALLPCASAVRPFVLVLSRDDFLKDTAGAHPSLPSADADSDE 76

Query: 62  WDEFGEPESHNPALE--LDPGTWRPIFEPDSTAAASDAPPDP----YYTALGKIISAVSS 121
           WD+F   +  +PA +  L P +W P+ +P S + + D P  P    +   +  ++SA S+
Sbjct: 77  WDDF---DDESPATDPLLSPSSWVPLLDPASASPSGDEPDSPSDALFVAGVRAMLSAASA 136

Query: 122 GDVRLMEDAVADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAE-GNKQ 181
           GD      A A ++  AA  G P AQS L  L G G+ +  ++ +AF+ H F+A+ G+ Q
Sbjct: 137 GDDAAFATAAAQIE-AAATGGHPGAQSALAFLSGAGMTRPASRSRAFLLHKFAADAGDLQ 196

Query: 182 SKMAVAYTYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRK 241
           SKMA+AY++FRQEMYE+AV LYAELAE A+ S L+SK+ PVIEPVR+H+G EENK+ALRK
Sbjct: 197 SKMALAYSFFRQEMYEEAVTLYAELAEAALTSSLISKEPPVIEPVRLHSGTEENKEALRK 256

Query: 242 SRGEEDDDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKS 301
           SRGE+D+DFQI EYQAQ+GN  AM+++GL YY+GLRG+RRD+ KA  WFSKAVEKG+ ++
Sbjct: 257 SRGEDDEDFQITEYQAQRGNTVAMHKLGLLYYYGLRGVRRDYGKAYHWFSKAVEKGDTRA 316

Query: 302 MELLGEIYARGAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVD-KNFTKAKE 361
           MELLGEIYARGAGVER+YT+A++WLT A+K   +SAYNG+GYLYVKGYGV+ KN TKAKE
Sbjct: 317 MELLGEIYARGAGVERNYTEAYKWLTLAAKQQQYSAYNGLGYLYVKGYGVEKKNLTKAKE 376

Query: 362 YFEKAANNEESGGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTG 421
           +FE AA ++E GG+YNLGV+YLKGIGVKRDV  AC  F+ A NAGQPKA YQ+AK+F  G
Sbjct: 377 FFEIAAEHKEHGGYYNLGVLYLKGIGVKRDVMTACNFFLRAVNAGQPKAIYQVAKLFQKG 436

Query: 422 VGLKRNIPMATALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSN 481
           VGLKRN+ MA  +YK VAERGPWSSLSRWALESYLK DIGKA  LY+RMA+LGYEVAQSN
Sbjct: 437 VGLKRNLQMAAVMYKSVAERGPWSSLSRWALESYLKGDIGKALLLYSRMADLGYEVAQSN 496

Query: 482 AAWILDKYGEQSICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTD 541
           AAWILD+YG++SIC+GESGFC+D ERH RAH+LWW ASEQGNEHAALLIGDAYYYGRG  
Sbjct: 497 AAWILDRYGDESICMGESGFCTDMERHLRAHALWWQASEQGNEHAALLIGDAYYYGRGVG 556

Query: 542 VDYDRAAEAYMHAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPV 601
            DY+RAAEAYMHA+SQ NAQAMFNLGYMHEHG GLP DLHLAKRYYDQA+E+DPAA+LPV
Sbjct: 557 RDYERAAEAYMHAQSQSNAQAMFNLGYMHEHGHGLPLDLHLAKRYYDQAVEVDPAAKLPV 616

Query: 602 KLALVSLWLRNNHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLY 661
            LAL SLW+R N+  SFLVH IDSLPEVYP ++ WVE+VL++EGNATI TLFACL+TVLY
Sbjct: 617 MLALTSLWIRKNYDGSFLVHFIDSLPEVYPVVEEWVEDVLMDEGNATIFTLFACLVTVLY 676

Query: 662 LRERQRRQAAVRAAAQAVPLQPN 670
           LRERQRRQAA      A P QP+
Sbjct: 677 LRERQRRQAAA-----ANPQQPD 690

BLAST of Sed0009438 vs. ExPASy Swiss-Prot
Match: Q9C6B6 (ERAD-associated E3 ubiquitin-protein ligase component HRD3B OS=Arabidopsis thaliana OX=3702 GN=HRD3B PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.7e-221
Identity = 399/624 (63.94%), Postives = 483/624 (77.40%), Query Frame = 0

Query: 13  LIISLCSLS-----ILARPFLIVISQDDLKDAALADDSAADSSDSAEWDEFGEPESHNPA 72
           + ISL +LS     + ARPF++V+S +DL +    D+ A +SSD   +DEFGE E  +  
Sbjct: 9   IAISLFTLSLYIHRVQARPFVLVLSNEDL-NGGFNDNGAYESSD---FDEFGESEPKSEE 68

Query: 73  LELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVADVDRLAAE 132
            ELDPG+WR IFE + +   + A P  YY+ L KI+SA S G+  LME+AV+++D  +A 
Sbjct: 69  -ELDPGSWRRIFETNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAVSEIDS-SAS 128

Query: 133 SGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAE-GNKQSKMAVAYTYFRQEMYEKAV 192
           SGDPHAQS++G +YG+G+M+E ++ K+ ++HHF+A  GN QSKMA+A+ Y RQ MY+KAV
Sbjct: 129 SGDPHAQSVMGFVYGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYLRQNMYDKAV 188

Query: 193 KLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKG 252
           +LYAELAE A+NS L+SKDSP+ EPVRIH G EENK ALRKSRGEED+DFQILEYQA+KG
Sbjct: 189 ELYAELAETAVNSFLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQILEYQAEKG 248

Query: 253 NAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGAGVERDYT 312
           N+ AM++IGLFYYFGLRGLRRDHAKAL WFSKA                           
Sbjct: 249 NSVAMHKIGLFYYFGLRGLRRDHAKALYWFSKA--------------------------- 308

Query: 313 KAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDK-NFTKAKEYFEKAANNEESGGHYNLGV 372
                            +NG+GYLYVKGYGVDK N+TKA+EYFE AANNE+  GHYNLGV
Sbjct: 309 ----------------EFNGLGYLYVKGYGVDKRNYTKAREYFEMAANNEDPSGHYNLGV 368

Query: 373 MYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAE 432
           +YLKG GVK+DV+ A  +F +AANAGQPKAFYQLAKMFHTGVGL +N+ MAT  YKLVAE
Sbjct: 369 LYLKGTGVKKDVRHATKYFFVAANAGQPKAFYQLAKMFHTGVGLTKNLEMATTFYKLVAE 428

Query: 433 RGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSICLGESG 492
           RGPWSSLSRWALE+YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE+S+C+G  G
Sbjct: 429 RGPWSSLSRWALEAYLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGERSMCMGVYG 488

Query: 493 FCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNA 552
           FC+D ERH RAHSLWW ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYM+AKSQ NA
Sbjct: 489 FCTDKERHDRAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMYAKSQSNA 548

Query: 553 QAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNHADSFLV 612
           QAMFNLGYMHEHG+GLPFDLHLAKRYYDQAL+ D AA+LPV LAL S+W+R N+AD+ LV
Sbjct: 549 QAMFNLGYMHEHGEGLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVRRNYADTALV 582

Query: 613 HVIDSLPEVYPKIDAWVEEVLLEE 630
            V++SLPEV+ K+  WVE  +LEE
Sbjct: 609 QVLNSLPEVHQKVVEWVENGMLEE 582

BLAST of Sed0009438 vs. ExPASy Swiss-Prot
Match: Q9Z2G6 (Protein sel-1 homolog 1 OS=Mus musculus OX=10090 GN=Sel1l PE=1 SV=2)

HSP 1 Score: 280.4 bits (716), Expect = 5.3e-74
Identity = 201/588 (34.18%), Postives = 311/588 (52.89%), Query Frame = 0

Query: 98  ALGKIISAVSSGDVRLME-DAVADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFM 157
           AL ++  A+  GD       A  ++     E G P  Q+ LG LY  G+    ++ KA +
Sbjct: 217 ALERVSYALLFGDYLTQNIQAAKEMFEKLTEEGSPKGQTGLGFLYASGLGVNSSQAKALV 276

Query: 158 YHHFSA-EGNKQSKMAVAYTYFR----QEMYEKAVKLYAELAEVAINSLLVSKDSPVIEP 217
           Y+ F A  GN  + M + Y Y+      +  E A+  Y  +A    + + ++  S V++ 
Sbjct: 277 YYTFGALGGNLIAHMILGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGGS-VVQR 336

Query: 218 VRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAK 277
           +R+    +E +     S   E+D  Q  ++ A+KG+  A   +G  +  G RG+ ++H +
Sbjct: 337 IRL---PDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQR 396

Query: 278 ALSWFSKAVEKGEPKSMELLGEIYARGAG-VERDYTKAFQWLTQASKHPAFSAYNGMGYL 337
           A  +F+ A   G   +M  LG++Y+ G+  V +    A  +  +A+        +G+G  
Sbjct: 397 AFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMA 456

Query: 338 YVKGYGVDKNFTKAKEYFEKAANNEESGGHYNLGVMYLKGIGVKRDVKKACTHFIMAANA 397
           Y+ G GV  N+  A +YF+KAA      G   LG MY  GIGVKRD K+A  +F +A+  
Sbjct: 457 YLYGRGVQVNYDLALKYFQKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQG 516

Query: 398 GQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAERGPWSSLSRWALESYLKADIGKAFF 457
           G   AFY LA+M  +G G+ R+   A  L+K V ERG WS     A  SY   D   A  
Sbjct: 517 GHILAFYNLAQMHASGTGVMRSCHTAVELFKNVCERGRWSERLMTAYNSYKDEDYNAAVV 576

Query: 458 LYARMAELGYEVAQSNAAWILDKYGEQSICLGESGFCSDAERHQRAHSLWWLASEQGNEH 517
            Y  +AE GYEVAQSNAA+ILD+   ++  +GE+      E + RA   W  A+ QG   
Sbjct: 577 QYLLLAEQGYEVAQSNAAFILDQ--REATIVGEN------ETYPRALLHWNRAASQGYTV 636

Query: 518 AALLIGDAYYYGRGTDVDYDRAAEAY-MHAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAK 577
           A + +GD ++YG GTDVDY+ A   Y + ++ Q +AQAMFNLGYMHE G G+  D+HLAK
Sbjct: 637 ARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDIHLAK 696

Query: 578 RYYDQALELDPAARLPVKLALVSLWLRNNHADSFLVHVID-SLPEVYPKIDAWVEEVLLE 637
           R+YD A E  P A++PV LAL  L +       FL ++ + ++ +++ ++D  ++++L  
Sbjct: 697 RFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYIREANIRDLFTQLD--MDQLLGP 756

Query: 638 EGNATILTLFACLL-TVLYLRERQRRQAAVRAAAQAVPLQPNDHGVPQ 676
           E +  ++T+ A LL TV+  R+RQ +   V       P  P   G P+
Sbjct: 757 EWDLYLMTIIALLLGTVIAYRQRQHQDIPVPRPPGPRPAPPQQEGPPE 785

BLAST of Sed0009438 vs. ExPASy Swiss-Prot
Match: Q80Z70 (Protein sel-1 homolog 1 OS=Rattus norvegicus OX=10116 GN=Sel1l PE=1 SV=2)

HSP 1 Score: 280.4 bits (716), Expect = 5.3e-74
Identity = 201/588 (34.18%), Postives = 311/588 (52.89%), Query Frame = 0

Query: 98  ALGKIISAVSSGDVRLME-DAVADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFM 157
           AL ++  A+  GD       A  ++     E G P  Q+ LG LY  G+    ++ KA +
Sbjct: 221 ALERVSYALLFGDYLTQNIQAAKEMFEKLTEEGSPKGQTGLGFLYASGLGVNSSQAKALV 280

Query: 158 YHHFSA-EGNKQSKMAVAYTYFR----QEMYEKAVKLYAELAEVAINSLLVSKDSPVIEP 217
           Y+ F A  GN  + M + Y Y+      +  E A+  Y  +A    + + ++  S V++ 
Sbjct: 281 YYTFGALGGNLIAHMVLGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGGS-VVQR 340

Query: 218 VRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAK 277
           +R+    +E +     S   E+D  Q  ++ A+KG+  A   +G  +  G RG+ ++H +
Sbjct: 341 IRL---PDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQR 400

Query: 278 ALSWFSKAVEKGEPKSMELLGEIYARGAG-VERDYTKAFQWLTQASKHPAFSAYNGMGYL 337
           A  +F+ A   G   +M  LG++Y+ G+  V +    A  +  +A+        +G+G  
Sbjct: 401 AFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMA 460

Query: 338 YVKGYGVDKNFTKAKEYFEKAANNEESGGHYNLGVMYLKGIGVKRDVKKACTHFIMAANA 397
           Y+ G GV  N+  A +YF+KAA      G   LG MY  GIGVKRD K+A  +F +A+  
Sbjct: 461 YLYGRGVQVNYDLALKYFQKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQG 520

Query: 398 GQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAERGPWSSLSRWALESYLKADIGKAFF 457
           G   AFY LA+M  +G G+ R+   A  L+K V ERG WS     A  SY   D   A  
Sbjct: 521 GHILAFYNLAQMHASGTGVMRSCHTAVELFKNVCERGRWSERLMTAYNSYKDDDYNAAVV 580

Query: 458 LYARMAELGYEVAQSNAAWILDKYGEQSICLGESGFCSDAERHQRAHSLWWLASEQGNEH 517
            Y  +AE GYEVAQSNAA+ILD+   ++  +GE+      E + RA   W  A+ QG   
Sbjct: 581 QYLLLAEQGYEVAQSNAAFILDQ--REATIVGEN------ETYPRALLHWNRAASQGYTV 640

Query: 518 AALLIGDAYYYGRGTDVDYDRAAEAY-MHAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAK 577
           A + +GD ++YG GTDVDY+ A   Y + ++ Q +AQAMFNLGYMHE G G+  D+HLAK
Sbjct: 641 ARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDIHLAK 700

Query: 578 RYYDQALELDPAARLPVKLALVSLWLRNNHADSFLVHVID-SLPEVYPKIDAWVEEVLLE 637
           R+YD A E  P A++PV LAL  L +       FL ++ + ++ +++ ++D  ++++L  
Sbjct: 701 RFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYIREANIRDLFTQLD--MDQLLGP 760

Query: 638 EGNATILTLFACLL-TVLYLRERQRRQAAVRAAAQAVPLQPNDHGVPQ 676
           E +  ++T+ A LL TV+  R+RQ +   V       P  P   G P+
Sbjct: 761 EWDLYLMTIIALLLGTVIAYRQRQHQDIPVPRPPGPRPAPPQQEGPPE 789

BLAST of Sed0009438 vs. ExPASy TrEMBL
Match: A0A0A0LQX2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042850 PE=4 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 617/679 (90.87%), Postives = 647/679 (95.29%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQLQ R+LQLIFLI+ L SL I ARPFLIVISQDDLKD A  DDS+ + +SDSA+WDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAA--SDAPPDPYYTALGKIISAVSSGDVRLMEDA 120
           EPES N ALELDPG+WRPIFEPDSTA+A  SDAP D YYTALGK++SAVSSGD+RLMEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSTASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYF 180
           VAD+D+  AESGDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YF
Sbjct: 121 VADIDQAVAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEES 360
           GAGVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAT 420
           GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540
           S+CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRN 600
           HAKSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRM 600

Query: 601 NHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660
           NHADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRR AAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAAV 660

Query: 661 RAAAQAVPLQPNDHGVPQN 677
           R AA+AVPL PNDH  PQN
Sbjct: 661 R-AAEAVPLHPNDHVPPQN 678

BLAST of Sed0009438 vs. ExPASy TrEMBL
Match: A0A5D3BIX0 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002400 PE=4 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 613/683 (89.75%), Postives = 646/683 (94.58%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQL+ R++QLIFLI+ L SL I ARPFLIVISQDDLKD A  DDS+ + +SDSA+WDEFG
Sbjct: 1   MQLETRRVQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAA--SDAPPDPYYTALGKIISAVSSGDVRLMEDA 120
           EPES N ALELDPG+WRPIFEPDS A+A  SDAP D YYTALGK++SAVSSGD+RLMEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSLASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYF 180
           VAD+D+  A++GDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YF
Sbjct: 121 VADIDQAVADNGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEES 360
           GAGVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAT 420
           GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540
           S+CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRN 600
           HAKSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRK 600

Query: 601 NHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660
           NHADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRRQAAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660

Query: 661 RAAAQAV----PLQPNDHGVPQN 677
           RAAA A     PL PNDH  PQN
Sbjct: 661 RAAAAAAEAVPPLHPNDHLPPQN 683

BLAST of Sed0009438 vs. ExPASy TrEMBL
Match: A0A1S3CMW9 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Cucumis melo OX=3656 GN=LOC103502593 PE=4 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 613/683 (89.75%), Postives = 646/683 (94.58%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSA-ADSSDSAEWDEFG 60
           MQL+ R++QLIFLI+ L SL I ARPFLIVISQDDLKD A  DDS+ + +SDSA+WDEFG
Sbjct: 1   MQLETRRVQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAA--SDAPPDPYYTALGKIISAVSSGDVRLMEDA 120
           EPES N ALELDPG+WRPIFEPDS A+A  SDAP D YYTALGK++SAVSSGD+RLMEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSLASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VADVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYF 180
           VAD+D+  A++GDPHAQS+LGLLYG+GIMKE NK KAFMYHHF+AEGNKQSKMA+AY YF
Sbjct: 121 VADIDQAVADNGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEES 360
           GAGVERDYTKA QWLT+ASK P+F+AYNGMGYLYVKGYGV+KN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAT 420
           GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMA+
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SICLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540
           S+CLGESGFC+DAERHQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRN 600
           HAKSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRK 600

Query: 601 NHADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660
           NHADSFLVHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLFACLLTVLYLRERQRRQAAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660

Query: 661 RAAAQAV----PLQPNDHGVPQN 677
           RAAA A     PL PNDH  PQN
Sbjct: 661 RAAAAAAEAVPPLHPNDHLPPQN 683

BLAST of Sed0009438 vs. ExPASy TrEMBL
Match: A0A6J1E8X2 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like OS=Cucurbita moschata OX=3662 GN=LOC111431721 PE=4 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 608/679 (89.54%), Postives = 641/679 (94.40%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSS-DSAEWDEFG 60
           MQLQ R++QLIFLII LCS  I ARPFLIVISQDDLKDAA  DDS+  ++ DSAEWDEFG
Sbjct: 5   MQLQARRIQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 64

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVA 120
           EPES N A ELDPGTWRPIFEPDS A+  DAP D YYTALGK+++AVSSGD+RLMEDAV 
Sbjct: 65  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 124

Query: 121 DVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYFRQ 180
           D+D++A ESGDPHAQS+LGLLYG+GIMKE NK KAFMYH+F+AEGNKQSKMA+AY+YFRQ
Sbjct: 125 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 184

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQIL
Sbjct: 185 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 244

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEPKSMELLGEIYARGA
Sbjct: 245 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 304

Query: 301 GVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEESGG 360
           GVERDYTKA QWLT+ASKHP+FSAYNG+GYLYVKGYGV+KNFTKAKEYFEKAANN+ESGG
Sbjct: 305 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 364

Query: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATAL 420
           HYNLGVMYLKGIGVKRDVKKACTHFI+AANAGQPKAFYQLAKMFHTGVGLKRNIPMA+AL
Sbjct: 365 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 424

Query: 421 YKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSI 480
           YKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQS+
Sbjct: 425 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 484

Query: 481 CLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540
           CLGESGFC+DAE HQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 485 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 544

Query: 541 KSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNH 600
           KSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLR NH
Sbjct: 545 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 604

Query: 601 ADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVR- 660
           ADSFLVHVIDSLPEVYPKID WVE+VLLEEGNATILTLFACLLTVLYLRERQRR A VR 
Sbjct: 605 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 664

Query: 661 -AAAQAVPLQPNDHGVPQN 677
            AAA+AVPL PNDH   QN
Sbjct: 665 AAAAEAVPLHPNDHVAAQN 683

BLAST of Sed0009438 vs. ExPASy TrEMBL
Match: A0A6J1KM40 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like OS=Cucurbita maxima OX=3661 GN=LOC111495816 PE=4 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 607/683 (88.87%), Postives = 641/683 (93.85%), Query Frame = 0

Query: 1   MQLQMRKLQLIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSS-DSAEWDEFG 60
           MQLQ R+LQLIFLII LCS  I ARPFLIVISQDDLKDAA  DDS+  ++ +SAEWDEFG
Sbjct: 13  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYESAEWDEFG 72

Query: 61  EPESHNPALELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVA 120
           EPES N A ELDPGTWRPIFEPDS A+  DAP D YYTALGK+++AVSSGD+RLMEDAV 
Sbjct: 73  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 132

Query: 121 DVDRLAAESGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAEGNKQSKMAVAYTYFRQ 180
           D+D++A ESGDPHAQS+LGLLYG+GIMKE NK KAFMYH+F+AEGNKQSKMA+AY+YFRQ
Sbjct: 133 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 192

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEED+DFQIL
Sbjct: 193 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 252

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WF+KAV+KGEPKSMELLGEIYARGA
Sbjct: 253 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFAKAVDKGEPKSMELLGEIYARGA 312

Query: 301 GVERDYTKAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDKNFTKAKEYFEKAANNEESGG 360
           GVERDYTKA QWLT+ASKHP+FSAYNG+GYLYVKGYGV+KNFTKAKEYFEKAANN+ESGG
Sbjct: 313 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 372

Query: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATAL 420
           HYNLGVMYLKGIGVKRDVKKACTHFI+AANAGQPKAFYQLAKMFHTGVGLKRNIPMA+AL
Sbjct: 373 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 432

Query: 421 YKLVAERGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSI 480
           YKLVAERGPWSSLSRWALESYLK+DIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQS+
Sbjct: 433 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 492

Query: 481 CLGESGFCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540
           CLGESGFC+DAE HQRAHSLWW ASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 493 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 552

Query: 541 KSQLNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNH 600
           KSQLNAQAMFNLGYMHEHG GLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLR NH
Sbjct: 553 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 612

Query: 601 ADSFLVHVIDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVR- 660
           ADSFLVHVIDSLPEVYPKID WVE+VLLEEGNATILTLFACLLTVLYLRERQRR A VR 
Sbjct: 613 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 672

Query: 661 -----AAAQAVPLQPNDHGVPQN 677
                AAA+AVPL PNDH   QN
Sbjct: 673 AAAAAAAAEAVPLHPNDHVAAQN 695

BLAST of Sed0009438 vs. TAIR 10
Match: AT1G18260.1 (HCP-like superfamily protein )

HSP 1 Score: 933.3 bits (2411), Expect = 1.1e-271
Identity = 467/670 (69.70%), Postives = 560/670 (83.58%), Query Frame = 0

Query: 10  LIFLIISLCSLSILARPFLIVISQDDLKDAALADDSAADSSDSAEWDEFGEPESHNPALE 69
           L  L+ S     + ARP ++V+S DDL      DD+     +S+++DEFGE E  +   E
Sbjct: 11  LSLLVFSFIEFGVHARPVVLVLSNDDLNSG--GDDNGV--GESSDFDEFGESEPKSEE-E 70

Query: 70  LDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVADVDRLAAESG 129
           LDPG+WR IFEPD +   + +P   YY+ L KI+SA S G+ RLME+AV +++  A+ +G
Sbjct: 71  LDPGSWRSIFEPDDSTVQAASP--QYYSGLKKILSAASEGNFRLMEEAVDEIE-AASSAG 130

Query: 130 DPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAE-GNKQSKMAVAYTYFRQEMYEKAVKL 189
           DPHAQS++G +YG+G+M+E +K K+F++H+F+A  GN QSKMA+A+TY RQ+M++KAV+L
Sbjct: 131 DPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDKAVQL 190

Query: 190 YAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKGNA 249
           YAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEED+DFQILEYQAQKGNA
Sbjct: 191 YAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQKGNA 250

Query: 250 GAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGAGVERDYTKA 309
            AMY+IGLFYYFGLRGLRRDH KAL WF KAV+KGEP+SMELLGEIYARGAGVER+YTKA
Sbjct: 251 NAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGAGVERNYTKA 310

Query: 310 FQWLTQASKHPAFSAYNGMGYLYVKGYGVD-KNFTKAKEYFEKAANNEESGGHYNLGVMY 369
            +WLT A+K   +SA+NG+GYLYVKGYGVD KN+TKA+EYFEKA +NE+  GHYNLGV+Y
Sbjct: 311 LEWLTLAAKEGLYSAFNGIGYLYVKGYGVDKKNYTKAREYFEKAVDNEDPSGHYNLGVLY 370

Query: 370 LKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAERG 429
           LKGIGV RDV++A  +F +AANAGQPKAFYQLAKMFHTGVGLK+N+ MAT+ YKLVAERG
Sbjct: 371 LKGIGVNRDVRQATKYFFVAANAGQPKAFYQLAKMFHTGVGLKKNLEMATSFYKLVAERG 430

Query: 430 PWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSICLGESGFC 489
           PWSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+S+C+G SGFC
Sbjct: 431 PWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGVSGFC 490

Query: 490 SDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQA 549
           +D ERH+RAHSLWW ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYMHAKSQ NAQA
Sbjct: 491 TDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQSNAQA 550

Query: 550 MFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNHADSFLVHV 609
           MFNLGYMHEHGQGLPFDLHLAKRYYD++L+ D AARLPV LAL SLWLR N+AD+ LV V
Sbjct: 551 MFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTVLVRV 610

Query: 610 IDSLPEVYPKIDAWVEEVLLEEGNATILTLFACLLTVLYLRERQRRQAAVRAAAQAVPL- 669
           +DSLPEVYPK++ W+E V+ EEGNATILTLF CL+T+LYLRERQRRQ  V A   A  + 
Sbjct: 611 VDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRERQRRQVVVVADPVAADVA 670

Query: 670 QPNDHGVPQN 677
           QP D  V Q+
Sbjct: 671 QPLDADVAQH 672

BLAST of Sed0009438 vs. TAIR 10
Match: AT1G73570.1 (HCP-like superfamily protein )

HSP 1 Score: 770.4 bits (1988), Expect = 1.2e-222
Identity = 399/624 (63.94%), Postives = 483/624 (77.40%), Query Frame = 0

Query: 13  LIISLCSLS-----ILARPFLIVISQDDLKDAALADDSAADSSDSAEWDEFGEPESHNPA 72
           + ISL +LS     + ARPF++V+S +DL +    D+ A +SSD   +DEFGE E  +  
Sbjct: 9   IAISLFTLSLYIHRVQARPFVLVLSNEDL-NGGFNDNGAYESSD---FDEFGESEPKSEE 68

Query: 73  LELDPGTWRPIFEPDSTAAASDAPPDPYYTALGKIISAVSSGDVRLMEDAVADVDRLAAE 132
            ELDPG+WR IFE + +   + A P  YY+ L KI+SA S G+  LME+AV+++D  +A 
Sbjct: 69  -ELDPGSWRRIFETNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAVSEIDS-SAS 128

Query: 133 SGDPHAQSMLGLLYGLGIMKEINKGKAFMYHHFSAE-GNKQSKMAVAYTYFRQEMYEKAV 192
           SGDPHAQS++G +YG+G+M+E ++ K+ ++HHF+A  GN QSKMA+A+ Y RQ MY+KAV
Sbjct: 129 SGDPHAQSVMGFVYGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYLRQNMYDKAV 188

Query: 193 KLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDDDFQILEYQAQKG 252
           +LYAELAE A+NS L+SKDSP+ EPVRIH G EENK ALRKSRGEED+DFQILEYQA+KG
Sbjct: 189 ELYAELAETAVNSFLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQILEYQAEKG 248

Query: 253 NAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGAGVERDYT 312
           N+ AM++IGLFYYFGLRGLRRDHAKAL WFSKA                           
Sbjct: 249 NSVAMHKIGLFYYFGLRGLRRDHAKALYWFSKA--------------------------- 308

Query: 313 KAFQWLTQASKHPAFSAYNGMGYLYVKGYGVDK-NFTKAKEYFEKAANNEESGGHYNLGV 372
                            +NG+GYLYVKGYGVDK N+TKA+EYFE AANNE+  GHYNLGV
Sbjct: 309 ----------------EFNGLGYLYVKGYGVDKRNYTKAREYFEMAANNEDPSGHYNLGV 368

Query: 373 MYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMATALYKLVAE 432
           +YLKG GVK+DV+ A  +F +AANAGQPKAFYQLAKMFHTGVGL +N+ MAT  YKLVAE
Sbjct: 369 LYLKGTGVKKDVRHATKYFFVAANAGQPKAFYQLAKMFHTGVGLTKNLEMATTFYKLVAE 428

Query: 433 RGPWSSLSRWALESYLKADIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSICLGESG 492
           RGPWSSLSRWALE+YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE+S+C+G  G
Sbjct: 429 RGPWSSLSRWALEAYLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGERSMCMGVYG 488

Query: 493 FCSDAERHQRAHSLWWLASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNA 552
           FC+D ERH RAHSLWW ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYM+AKSQ NA
Sbjct: 489 FCTDKERHDRAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMYAKSQSNA 548

Query: 553 QAMFNLGYMHEHGQGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRNNHADSFLV 612
           QAMFNLGYMHEHG+GLPFDLHLAKRYYDQAL+ D AA+LPV LAL S+W+R N+AD+ LV
Sbjct: 549 QAMFNLGYMHEHGEGLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVRRNYADTALV 582

Query: 613 HVIDSLPEVYPKIDAWVEEVLLEE 630
            V++SLPEV+ K+  WVE  +LEE
Sbjct: 609 QVLNSLPEVHQKVVEWVENGMLEE 582