Sed0009354 (gene) Chayote v1

Overview
NameSed0009354
Typegene
OrganismSechium edule (Chayote v1)
Descriptionfatty acid hydroxylase 2
LocationLG10: 318325 .. 320064 (-)
RNA-Seq ExpressionSed0009354
SyntenySed0009354
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCAAAGGAATTTACTGTAGATCTTAAAAAACCACTTGTGTTCCAGGTAACTAAACACAAGCTATATTATATGGTTCTTTATAATTAATTTAAAGGAGACAATTGCTAAATTAGTTGTTGTGTTTGTACTCAAATTTGATATTATATTTATATATAGGTTGGTCATCTTGGGGAAGCATATCAAGAGTGGGTTCACCAAGGAATTGTCACCAAGGAAGTACCCCGATTCTTCAAAAGCGACTTAGCGGAGGTCCTATGAATTCATTATTAATATGCATGTCCTTTTTCTCTACTGCAGTAAAGGACTATATATAATGTGTCACATCTTCAAATATCATTTTCAATAACACGCAGGACTGTGTTTTTTGGTTTCAGTTTTTTACGCGCACAACATGGTGGGTGATTCCACTTATATGGGTGCCAGTGTCGTGTTGGTTCATTTCCATGTCTGTGAATACGGGGCACCACACGGTTGCGGAGATATCGATGATGGTCATCTTCAGCATCATTTTTATGTGGTCATTGGTCGAATACGTATTGCATCGTTTCCTATTCCATATGGACACCAACACTTACTGGACAAACACCTTACATTATTTCATTCATGGCTACCACCACAAGCACCCGATGGACACTCTCCGACTTGTCTTTCCTCCGGCCGGGACAGCTGCGCTGTTATTACCAGTATGTTCGCTCTCAGCTGTTATTATTATATATTCATAGAACATTAATTATAAGCTGATCAGATTGCATTGTATATATGTTGTTGTTTTGTATCCAGGTGTGGATCTTGATTAAACTCATTTTCCCGCTGTCTGTGGCCTCTGCTCTAACGGGAGGCCTTGTTTTGGGTTATGTCATCTATGACGTTACCCATTACTACTTGCATCACGGCCAACCCACCGGACACATCTCCAAAAATCTTAAGGTATTTAAAATCACATACCTTCAACCAACGGGTTAGAGGTTTGAATCTCATCTCCGCCTCCACATGTTGTAGTCGATATATTTAAATCACTAAACCTAAGGGCACTATTCGTTTGTCGTATAGGACTAGAAATGAAATTGAAGGTCGCCTGAAAAAATTAATACCCACAACTGCCTCAAACCTGAAATCTCAGGGTGGAACATGCCTTCCTTGACCCCTTGAGGACATTATCAGAATGATTATGAGGGAACAGTTCTTATCCACGTTTGGACCAACAAGAATCATTCCCTTATAATTAATATTCATTTTCTCCATAATTTCATCCTTCCCTCTAACTATTTTTTGTTCTTTCTATAACTACTTTTCATTTTCTCACACCCACTAATTATTCTCTATTATCATTCCTTGAAACACATACGTACGATCATTCCTTTCACAATCGTTCTCTACGCTCCAAATATAGATAAACATTTTCTTCATCTCAAACATAGATAATTATTCTCCCTCGTGTTCTTCTCCTGCCACCACTATCATTCTTCTTGCCCCAAACAAACCCGAGTGAAAATGTTGAGCATCCAAAGCATCAGCTGACAGCTATTGGATCATTGTAACTAAGTACTGTTTTATTTATGTTTTGAGTTGGAGGGACATTAAATATTGGGGTTGTGATGTGATGTGCAGAAATATCATTTGAATCATCACTTCCGCATCAACAACAAAGGCTTTGGCCTTACTTCATCAGTTTGGGACGCGGCGTTTGGGACTGTACCTACGACAAAAGCAGCTGATCAGAAGAAGACAAAGTAG

mRNA sequence

ATGGCTGCAAAGGAATTTACTGTAGATCTTAAAAAACCACTTGTGTTCCAGGTTGGTCATCTTGGGGAAGCATATCAAGAGTGGGTTCACCAAGGAATTGTCACCAAGGAAGTACCCCGATTCTTCAAAAGCGACTTAGCGGAGTTTTTTACGCGCACAACATGGTGGGTGATTCCACTTATATGGGTGCCAGTGTCGTGTTGGTTCATTTCCATGTCTGTGAATACGGGGCACCACACGGTTGCGGAGATATCGATGATGGTCATCTTCAGCATCATTTTTATGTGGTCATTGGTCGAATACGTATTGCATCGTTTCCTATTCCATATGGACACCAACACTTACTGGACAAACACCTTACATTATTTCATTCATGGCTACCACCACAAGCACCCGATGGACACTCTCCGACTTGTCTTTCCTCCGGCCGGGACAGCTGCGCTGTTATTACCAGTGTGGATCTTGATTAAACTCATTTTCCCGCTGTCTGTGGCCTCTGCTCTAACGGGAGGCCTTGTTTTGGGTTATGTCATCTATGACGTTACCCATTACTACTTGCATCACGGCCAACCCACCGGACACATCTCCAAAAATCTTAAGAAATATCATTTGAATCATCACTTCCGCATCAACAACAAAGGCTTTGGCCTTACTTCATCAGTTTGGGACGCGGCGTTTGGGACTGTACCTACGACAAAAGCAGCTGATCAGAAGAAGACAAAGTAG

Coding sequence (CDS)

ATGGCTGCAAAGGAATTTACTGTAGATCTTAAAAAACCACTTGTGTTCCAGGTTGGTCATCTTGGGGAAGCATATCAAGAGTGGGTTCACCAAGGAATTGTCACCAAGGAAGTACCCCGATTCTTCAAAAGCGACTTAGCGGAGTTTTTTACGCGCACAACATGGTGGGTGATTCCACTTATATGGGTGCCAGTGTCGTGTTGGTTCATTTCCATGTCTGTGAATACGGGGCACCACACGGTTGCGGAGATATCGATGATGGTCATCTTCAGCATCATTTTTATGTGGTCATTGGTCGAATACGTATTGCATCGTTTCCTATTCCATATGGACACCAACACTTACTGGACAAACACCTTACATTATTTCATTCATGGCTACCACCACAAGCACCCGATGGACACTCTCCGACTTGTCTTTCCTCCGGCCGGGACAGCTGCGCTGTTATTACCAGTGTGGATCTTGATTAAACTCATTTTCCCGCTGTCTGTGGCCTCTGCTCTAACGGGAGGCCTTGTTTTGGGTTATGTCATCTATGACGTTACCCATTACTACTTGCATCACGGCCAACCCACCGGACACATCTCCAAAAATCTTAAGAAATATCATTTGAATCATCACTTCCGCATCAACAACAAAGGCTTTGGCCTTACTTCATCAGTTTGGGACGCGGCGTTTGGGACTGTACCTACGACAAAAGCAGCTGATCAGAAGAAGACAAAGTAG

Protein sequence

MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPLIWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTLHYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYDVTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKKTK
Homology
BLAST of Sed0009354 vs. NCBI nr
Match: XP_023518676.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] >XP_023518677.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 364.4 bits (934), Expect = 7.6e-97
Identity = 167/239 (69.87%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+PL
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 61  IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA L++P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. NCBI nr
Match: XP_023004067.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima] >XP_023004068.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima])

HSP 1 Score: 364.0 bits (933), Expect = 1.0e-96
Identity = 167/239 (69.87%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+P+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 61  IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA LL+P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLLVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. NCBI nr
Match: XP_022926333.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_022926334.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 1.7e-96
Identity = 166/239 (69.46%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+P+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 61  IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA L++P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. NCBI nr
Match: KAG6594413.1 (Dihydroceramide fatty acyl 2-hydroxylase FAH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 363.2 bits (931), Expect = 1.7e-96
Identity = 166/239 (69.46%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+P+
Sbjct: 48  MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 107

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 108 IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 167

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA L++P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 168 HYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 227

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 228 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 284

BLAST of Sed0009354 vs. NCBI nr
Match: XP_038882766.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 5.0e-96
Identity = 168/239 (70.29%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQEWVHQ I++KE PRFFKSD  EF TRT WW++PL
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTRTAWWMVPL 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWF+SMSVN G HT+ +IS+MVIF  I +W+L+EY++HRFLFH++T +YW NT+
Sbjct: 61  IWLPVVCWFVSMSVNMG-HTLPQISLMVIFG-ISIWTLIEYIVHRFLFHIETQSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA LL+P+W L+KL FP S+A AL GG +LGYVIYD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLSFPPSMAPALFGGGLLGYVIYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K LKKYH+NHHFRI NKGFG+TS +WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGDVPKKLKKYHMNHHFRILNKGFGVTSPLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. ExPASy Swiss-Prot
Match: O48916 (Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN=FAH1 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.0e-84
Identity = 153/234 (65.38%), Postives = 178/234 (76.07%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+ FTVDLKKPLVFQVGHLGE Y+EWVHQ I TKE PRFF+SD  EF T T WW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV  W IS SV+ G  ++ EI  +V+   IF+W+  EYVLHRF+FH+ T +YW NT 
Sbjct: 61  IWLPVVVWCISRSVSMG-CSLPEIVPIVVMG-IFIWTFFEYVLHRFVFHIKTKSYWGNTA 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD LRLVFPP  TA L  P W + K I   S A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKA 235
           VTHYYLHH QPT  ++KNLKKYHLNHHFRI +KGFG+TSS+WD  FGT+PTTKA
Sbjct: 181 VTHYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKA 232

BLAST of Sed0009354 vs. ExPASy Swiss-Prot
Match: Q9SUC5 (Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN=FAH2 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.2e-82
Identity = 143/238 (60.08%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+ +TVDL KPLVFQVGHLGE YQEW+HQ IV  E PRFF+SD  EF TRT WW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV C+ +S+S + G  T  +I ++V F ++  W+L+EY LHRFLFH+ T +YW NT 
Sbjct: 61  IWLPVVCYVLSISASKG-LTFPQIGLIVAFGVL-TWTLLEYTLHRFLFHIQTKSYWANTA 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY +HG HHKHP D LRLVFPP  TA LL+P+W L+ L+   + A A+ GG++ GYV+YD
Sbjct: 121 HYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQK 239
           +THYYLHHGQP     K+LKKYHLNHHFRI +KG+G+TSS+WD  FGT+P  KAA +K
Sbjct: 181 ITHYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAKK 236

BLAST of Sed0009354 vs. ExPASy Swiss-Prot
Match: Q4R4P4 (Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-45
Identity = 102/241 (42.32%), Postives = 147/241 (61.00%), Query Frame = 0

Query: 8   VDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPLIWVPVSC 67
           VD +KPL++QVGHLGE Y EWVHQ  VT+ + R F SDL E  ++T W+ +P+IWVP+  
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQP-VTRPI-RLFHSDLIEGLSKTVWYSVPIIWVPLVL 183

Query: 68  W--------FISMSVNTGHHTVAEISMMVIFSI--------IFMWSLVEYVLHRFLFHM- 127
           +        F   +V        E ++ V  S+        IF+WSL+EY++HRFLFHM 
Sbjct: 184 YLSWSYYRTFAQGNVRLFTSFTTEYALAVPKSMFPGLFMLGIFLWSLIEYLIHRFLFHMK 243

Query: 128 -DTNTYWTNTLHYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALT 187
             +++Y+   LH+ +HG HHK P D  RLVFPP   + ++   ++ ++LI P +V   + 
Sbjct: 244 PPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCLQLILPEAVGGTVF 303

Query: 188 GGLVLGYVIYDVTHYYLHHGQP-TGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGT 230
            G +LGYV+YD+THYYLH G P  G    NLK +H+ HHF     GFG+++ +WD  F T
Sbjct: 304 AGGLLGYVLYDMTHYYLHFGSPHRGSYLYNLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 362

BLAST of Sed0009354 vs. ExPASy Swiss-Prot
Match: Q2LAM0 (Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-45
Identity = 99/241 (41.08%), Postives = 144/241 (59.75%), Query Frame = 0

Query: 8   VDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPLIWVPVSC 67
           VD +KPL++QVGHLGE Y EWVHQ +      R F SDL E F++T W+ +P+IWVP+  
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVA--RPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 183

Query: 68  WF--------------ISMSVNTGHHTVAEISMMVIFSIIFM--WSLVEYVLHRFLFHM- 127
           +               +  S    +  V   S+ +   ++ M  W+LVEY++HRFLFHM 
Sbjct: 184 YLSWSYYRTLTQDNIRLFASFTRDYSLVVPESVFIGLFVLGMLIWTLVEYLIHRFLFHMK 243

Query: 128 -DTNTYWTNTLHYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALT 187
             +N+++   LH+ +HG HHK P D  RLVFPP   + ++   ++ ++LI P +VA  L 
Sbjct: 244 PPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASVVVAFFYVFLRLILPEAVAGILF 303

Query: 188 GGLVLGYVIYDVTHYYLHHGQP-TGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGT 230
            G +LGYV+YD+THYYLH G P  G    N+K +H+ HHF     GFG+++ +WD  F T
Sbjct: 304 AGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKAHHVKHHFEYQKSGFGISTKLWDYFFHT 362

BLAST of Sed0009354 vs. ExPASy Swiss-Prot
Match: Q5MPP0 (Fatty acid 2-hydroxylase OS=Mus musculus OX=10090 GN=Fa2h PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 5.4e-45
Identity = 98/241 (40.66%), Postives = 142/241 (58.92%), Query Frame = 0

Query: 8   VDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPLIWVPVSC 67
           VD +KPL++QVGHLGE Y EWVHQ +      R F SDL E F++T W+ +P+IWVP+  
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVA--RPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 183

Query: 68  W--------FISMSVNTGHHTVAEISMMVIFSI--------IFMWSLVEYVLHRFLFHM- 127
           +            ++        E SMM+  S+        +  W+ VEYV+HRFLFHM 
Sbjct: 184 YLSWSYYRTLTQDNIRLFASLTREYSMMMPESVFIGLFVLGMLFWTFVEYVIHRFLFHMK 243

Query: 128 -DTNTYWTNTLHYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALT 187
             +N+++   LH+ +HG HHK P D  RLVFPP   + ++   ++ ++LI P +V   + 
Sbjct: 244 PPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIAFFYVFLRLILPETVGGIIF 303

Query: 188 GGLVLGYVIYDVTHYYLHHGQP-TGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGT 230
            G +LGYV+YD+THYYLH G P  G    N+K +H+ HHF     GFG+++ +WD  F T
Sbjct: 304 AGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKAHHVKHHFEYQKSGFGISTKLWDYFFHT 362

BLAST of Sed0009354 vs. ExPASy TrEMBL
Match: A0A6J1KYF5 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497491 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.8e-97
Identity = 167/239 (69.87%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+P+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 61  IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA LL+P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLLVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. ExPASy TrEMBL
Match: A0A6J1EET4 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111433515 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 8.2e-97
Identity = 166/239 (69.46%), Postives = 205/239 (85.77%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+EFTVDL KPLVFQVGHLGEAYQ+WVH+ IV+KE PRFFKSD  EF TRT WWV+P+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWFISMS+N G HT+ +IS MVIF  I++W+L+EY++HRFLFH++T++YW NT+
Sbjct: 61  IWLPVVCWFISMSMNMG-HTLGQISFMVIFG-IYIWTLIEYIVHRFLFHIETSSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA L++P+W L+KL FP S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFRI NKGFG+TSS+WD AFGT+P +K A++ +
Sbjct: 181 VTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKVAEKTR 237

BLAST of Sed0009354 vs. ExPASy TrEMBL
Match: A0A1S3AZM0 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo OX=3656 GN=LOC103484299 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.5e-94
Identity = 164/239 (68.62%), Postives = 198/239 (82.85%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M +++FTVDL KPLVFQVGHLGEAYQEWVHQ I++KE PRFFKSD  EF T T WW +PL
Sbjct: 1   MVSQQFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTCTAWWAVPL 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWF+SMSVN G HT ++IS+MVIF  I +W+L+EY++HRFLFH++T +YW NT+
Sbjct: 61  IWLPVVCWFVSMSVNMG-HTFSQISLMVIFG-ISIWTLIEYIVHRFLFHIETQSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA LL+P+W L+KL FP S+A AL GG +LGYVIYD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLFFPSSMAPALFGGGLLGYVIYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K LKKYH+NHHFRI NKGFG+TS +WD  FGT+P +K A + +
Sbjct: 181 VTHYYLHHGQPTGDMPKKLKKYHMNHHFRILNKGFGVTSPLWDRVFGTLPGSKVAQKTR 237

BLAST of Sed0009354 vs. ExPASy TrEMBL
Match: A0A5A7U9P5 (Dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00710 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.1e-93
Identity = 164/239 (68.62%), Postives = 198/239 (82.85%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M +++FTVDL KPLVFQVGHLGEAYQEWVHQ I++KE PRFFKSD  EF T T WW +PL
Sbjct: 1   MVSQQFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTCTAWWAVPL 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV CWF+SMSVN G HT ++IS+MVIF  I +W+L+EY++HRFLFH++T +YW NT+
Sbjct: 61  IWLPVVCWFVSMSVNMG-HTFSQISLMVIFG-ISIWTLIEYIVHRFLFHIETQSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD+LRLVFPP  TA LL+P+W L+KL FP S+A AL GG +LGYVIYD
Sbjct: 121 HYLIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLFFPPSMAPALFGGGLLGYVIYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKK 240
           VTHYYLHHGQPTG + K LKKYH+NHHFRI NKGFG+TS +WD  FGT+P +K A + +
Sbjct: 181 VTHYYLHHGQPTGDMPKKLKKYHMNHHFRILNKGFGVTSPLWDRVFGTLPGSKVAQKTR 237

BLAST of Sed0009354 vs. ExPASy TrEMBL
Match: A0A6J1CXN8 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=3673 GN=LOC111015759 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.2e-93
Identity = 165/241 (68.46%), Postives = 198/241 (82.16%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M ++EFTVDL KPLVFQVG+LGEAYQEWVHQ IV++E PRFFKSD  EF TRT WWVIPL
Sbjct: 1   MVSQEFTVDLNKPLVFQVGNLGEAYQEWVHQPIVSREGPRFFKSDFWEFLTRTAWWVIPL 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           +W+PV CWFIS SVN G HT+ +I++MVIF  I +W+L EY +HRFLFH+ T +YW NT+
Sbjct: 61  VWLPVVCWFISTSVNMG-HTLPQITLMVIFG-ISIWTLFEYTVHRFLFHIKTKSYWGNTM 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD LRLVFPPA TA L++P+W L+KL  P S+A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDGLRLVFPPAATAVLIVPLWNLVKLFAPPSIAPALFGGGLLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQKKT 240
           VTHYYLHHGQPTG + K+LKKYH+NHHFR+  KGFG+TSS WD AFGT+P +K A  +KT
Sbjct: 181 VTHYYLHHGQPTGDVPKHLKKYHMNHHFRVQTKGFGITSSFWDRAFGTLPASKVATIEKT 239

Query: 241 K 242
           +
Sbjct: 241 R 239

BLAST of Sed0009354 vs. TAIR 10
Match: AT2G34770.1 (fatty acid hydroxylase 1 )

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-85
Identity = 153/234 (65.38%), Postives = 178/234 (76.07%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+ FTVDLKKPLVFQVGHLGE Y+EWVHQ I TKE PRFF+SD  EF T T WW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV  W IS SV+ G  ++ EI  +V+   IF+W+  EYVLHRF+FH+ T +YW NT 
Sbjct: 61  IWLPVVVWCISRSVSMG-CSLPEIVPIVVMG-IFIWTFFEYVLHRFVFHIKTKSYWGNTA 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY IHG HHKHPMD LRLVFPP  TA L  P W + K I   S A AL GG +LGYV+YD
Sbjct: 121 HYLIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKA 235
           VTHYYLHH QPT  ++KNLKKYHLNHHFRI +KGFG+TSS+WD  FGT+PTTKA
Sbjct: 181 VTHYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKA 232

BLAST of Sed0009354 vs. TAIR 10
Match: AT4G20870.1 (fatty acid hydroxylase 2 )

HSP 1 Score: 305.8 bits (782), Expect = 3.0e-83
Identity = 143/238 (60.08%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 1   MAAKEFTVDLKKPLVFQVGHLGEAYQEWVHQGIVTKEVPRFFKSDLAEFFTRTTWWVIPL 60
           M A+ +TVDL KPLVFQVGHLGE YQEW+HQ IV  E PRFF+SD  EF TRT WW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  IWVPVSCWFISMSVNTGHHTVAEISMMVIFSIIFMWSLVEYVLHRFLFHMDTNTYWTNTL 120
           IW+PV C+ +S+S + G  T  +I ++V F ++  W+L+EY LHRFLFH+ T +YW NT 
Sbjct: 61  IWLPVVCYVLSISASKG-LTFPQIGLIVAFGVL-TWTLLEYTLHRFLFHIQTKSYWANTA 120

Query: 121 HYFIHGYHHKHPMDTLRLVFPPAGTAALLLPVWILIKLIFPLSVASALTGGLVLGYVIYD 180
           HY +HG HHKHP D LRLVFPP  TA LL+P+W L+ L+   + A A+ GG++ GYV+YD
Sbjct: 121 HYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYD 180

Query: 181 VTHYYLHHGQPTGHISKNLKKYHLNHHFRINNKGFGLTSSVWDAAFGTVPTTKAADQK 239
           +THYYLHHGQP     K+LKKYHLNHHFRI +KG+G+TSS+WD  FGT+P  KAA +K
Sbjct: 181 ITHYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAKK 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023518676.17.6e-9769.87dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] ... [more]
XP_023004067.11.0e-9669.87dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima] >XP_023004... [more]
XP_022926333.11.7e-9669.46dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_0229... [more]
KAG6594413.11.7e-9669.46Dihydroceramide fatty acyl 2-hydroxylase FAH1, partial [Cucurbita argyrosperma s... [more]
XP_038882766.15.0e-9670.29dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X2 [Benincasa hispida... [more]
Match NameE-valueIdentityDescription
O489162.0e-8465.38Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUC54.2e-8260.08Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q4R4P41.9e-4542.32Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1[more]
Q2LAM01.9e-4541.08Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2[more]
Q5MPP05.4e-4540.66Fatty acid 2-hydroxylase OS=Mus musculus OX=10090 GN=Fa2h PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KYF54.8e-9769.87dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1EET48.2e-9769.46dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662... [more]
A0A1S3AZM08.5e-9468.62dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7U9P51.1e-9368.62Dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo var. makuwa O... [more]
A0A6J1CXN83.2e-9368.46dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=367... [more]
Match NameE-valueIdentityDescription
AT2G34770.11.4e-8565.38fatty acid hydroxylase 1 [more]
AT4G20870.13.0e-8360.08fatty acid hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 92..228
e-value: 3.6E-14
score: 53.3
IPR014430Sterol desaturase Scs7PANTHERPTHR12863FATTY ACID HYDROXYLASEcoord: 3..238
NoneNo IPR availablePANTHERPTHR12863:SF1FATTY ACID 2-HYDROXYLASEcoord: 3..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0009354.1Sed0009354.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0008610 lipid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0080132 fatty acid alpha-hydroxylase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity