Sed0008819 (gene) Chayote v1

Overview
NameSed0008819
Typegene
OrganismSechium edule (Chayote v1)
DescriptionLaccase
LocationLG04: 26277278 .. 26281368 (+)
RNA-Seq ExpressionSed0008819
SyntenySed0008819
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCTCAAAAGTCCAACACAAATATTAAAACAGAAATGGCTTCTTTCTCTTTGAAAGCCATATTTTTCTTTGCCTTTTCTGTTCTTCTCTGTTTTCTTCCAAACATGGTCAATGCAGGCATCACAAGGCACTACAATTTTCATGTGAGCCCATTTTCTTACCTCTTGCATTGCCAAATTAATGCAACTTTCACTCAAATCATTCCTTTTTTTTGTTCCTTTTCAGATTCAATTGCAAAATGTAACAAGACTCTGTCGAACAAAGACCATTGTGACCGTCAACGGGCAGTTTCCCGGGCCGAGAATCATCGCTAGGGAAGGCGACCGCCTCTTGATCAAAGTGGTCAATCACGTCCAAAACAATATATCTCTCCATTGGTATGAAACATAGCCCATATCTCAAATCATTATTAGTCTAAAAAACTTAAACTAATGGATTAGGATACACGTACATATTACAATAGGCACGGAGTGCGACAGATGAGGAGCGGGTGGGCAGACGGGCCAGCGTACGTGACGCAATGCCCGATCCAAACAGGGCAGAGCTATGTGTATAACTTCACATTGAATGGGCAGAGAGGGACATTGTTTTGGCATGCTCATATCTCATGGCTAAGATCAACTGTGTATGGGCCAATCATCATTCTTCCCAAAACCAATCAGCCATACCCTTTTCCTCAACCTTTTGAAGAAATTCCCATTGTTTTTGGTAAGAGTTTTTGTTTGAAATTTTCAAGTTCTTTATTGAAAAGATTTGGTATTTGAATTATATGGGCATTGGGATTTAATTTGTAGGGGAGTGGTGGAAGGCTGATACTGAGGCTGTCATAAACCAAGCAATGAAAAGTGGTGGTGCTCCAAATATTTCTGATGCTTTTACCTTTAATGGCCTCCCTGGTCCCTCCTATAATTGCTCTGCTCAAGGTACTCCAAAATTCTTGCTTAATTCTGTTTCCTTTCTTACTTCATAATTTTGCAAATTATATGGCCAAAAACAGCCTCTAGTAGTTGCATCAATACATTAGCTTGAACATAGTTTAATTGAGAGTAATGTACTCATTTTGAATGCTAATTTTGGAGAAAAAGAATTAGTAACATATATAAGATTTGACTAGAACACCACTTGTATTTTTATTTGTTAATAAATCTCTCCTCTAGCTTTTTTCTTCTTTAACCTTAGATTTTTGCAATAATATAAATGCTGAATAAGGGAGGAATGAGAGAATTTTCAACCATCCACAACGTTTTTTTTAAATGTAAAACGTTGATTAAAAATATGGTAAAAATGTTAATGTTTATGAATATTTATAGAAAATCATAAAGATTAAAATTACAAAAGTGTTTAAATTGATAAATAAAATTTAACACTTTTAAATAAGTTAAAATGTTTATTTTTAATATTATGTTCACATTTGTGACATTACAATATTGGGTTGCATTAAGAATATTGATTCATTTCTTGTATTAATTCATCGAATATATCTCGTCTCTCTTATAAATGCACATTTATTGTAAATTACTAAAATATTCATATCATGAAAGTAGAGTTTCAATGATTACTTATACCACTTGGTATTGAAGAGGCCAGATATTTGAAACTTCCATCTGAAATGTGATTGAAGTAAAAAAAATAAAATCATAGTTTCAATTAATATTAAAATATAAATATGTTGATTTTGAAATATAAGTTTGAAATGGCAATGCAGATACCTTCAAGCTCAAGGTAAAACCAGGCAAATCCTATCTTCTCCGACTGATCAACGCTGCCCTCAATGACGAGCTCTTCTTCAGCATCGCCAACCACACACTCACCGTCGTCGAGGCCGACGCCATTTACGTCAAGCCCTTCGTAACCGACGTCCTTTTGATGGCTCCAGGACAAACCATGAACGTACTACTCCAAGCCAAGGCCAACGCTCCCAACGCCACATTCCTCATCGCGGCCCGGCCCTACGCCGTCGGGCCTGCCCCGTTCGACAACACGACCGTAACCGGCCTGCTCGAATACGAACCCAAAAAATCCCTATCAAAAAACAACAACAAGCTCCCTCTCCTAAAACCGGCCCTTCCCCGGTTCAACGACTCGAGTTTTTCCATCAAATTTAACGGGAAGATTCGGAGGCTGGTGAGTTCGAGATTCCCGGCGAAAGTTCCGACGAGGGTTGACCGGAGATTCTTTTTCACGGTGGGTTTGGGGGCGCTGCCGTGCGGGAGAAACGGATCGTGTCAGGGCCCGAACAATTTGAGATTCTCGGCATCGATTAATAACGTGACGTTTGTGCAGCCAAGTACGGCTCTTTTGCAAGCTCATTTCTTTAACAAATCTAACGGCGTTTATACCACTGATTTGCCGGTCAATCCGCCGTTTAAATTTAACTACACAGGTAAACTCCGGCGAGCCAATTCTCTCTGTTTCTCCTAGTTTTTAATTTCATTTTTTTTTATTATTCTGTCGAAGAGGGCTGAGCCTTGAGTTAGTTAATAGGCAGGTCCATGTTTCTATCTCAACTCTCAAATTGTTTTAACTTTTTTTTTCCCTTCAAGTTAAAAAATATGACAAAAACTTGCCTCTCCAATGATGAGGCACTCTTTAATTAAAATTTATAGGTCACATAGTTATTTATTTTTATAAATAATATTTAAAATTAAAAAAATTGTGTAGCGCGGATGATGCCGAATACATGACCTCTTTACTCAGAAGAGTCTTAAATTTTTCTCAAAAATATTTAGTGGTGTGAACATTTGACTTCAGGATCTATAATACATCTAATTATAGTTTTTTTAATAAAAAAAAAAAAAAAATTTCAACCATATTTATTGGTGTTAAAATCAAACTTATAATTTTTTGGTTATTAAATAGTACACCATATTAATTGGATTCAACTGACCAAGTTTTACACTAGTTTCACAAATTTAATGTTCTAAATTCATAAACTTTTAATATTGTGTCTATTTTTTTTTTGTTGAGTTATGCAATAATTAAAGATAAGGAAATTTGAAAATATGGTATCTAAAACAATTTATAATCACCCAATATTTTTTAATTAAAAAGGAAAAAAGAAAAATTTTAAAAAACATTTTATATTAAGTAAAACCTTCCTATAGTGGCACCCTTGAAAATAGTATCATGATTTAATATTGAAAATTAAAAAAAAAAGTTAGATTTAAAAAAAAGAGAAATACTTCCCTCCTTGCTTATAATTTGCTTAAAACGAGGGTTCCTTCTTTAAAGGTATATATGGTACCGTTTGATGGGACAAAATTTTTTTTTTTTTTTACTTTTTCCTTAAAATTGATTAAAAAAAATTTGTAATATGAAATGGTGACCACATATAGTCTTGAGGAGGGGCCCGAAAAAAAAAGTTGATTAATGAGTATGTAGTATTTCAGTTTAGAAAGGAAAATACTTGATTAGAGAGTATGTAGTGATCCATGTAAGGAAAGGTTGTTAAGAAAATTTGTTACATTTGGGTTTGTGCAGGCTCGCCGCCGAAGAACTTGGTAGTAAGCAGCGGAACAAAGGTGGCGGTGGTGCCGTACAACGCGGGCGTGGAAGTAGTAATGCAGGACACGAGCATAATCACGGCGGAGAGCCACCCGCTCCACCTGCACGGCTTCAATTTCTTCGTCTTAGCCCAAGGAATCGGCAATTTCGACCCCAGCAGAGACCCGGCCAGGTTCAACCTCGCAGACCCCCCCGAGAGGAACACCGTCGGCGTCCCCTCCGGCGGCTGGGTCGCCGTCCGGTTCGTCGCCGACAATCCCGGGGCTTGGTTTATGCACTGCCACTTGGAGGTTCACACCAGTTGGGGCCTCAAAATGGCCTGGATTGTGCAGGACGGAAACCGCCCCGATCAGAAGCTCCCGCCGCCGCCCCCCGATTTGCCCAAATGCTAACTGTTTTTCTTTTTTTTCTTCCGAAACATTTCCAAACGGAAAACCAGAAAGATTTCAAGATCATCCTTTTCGTATTCAATTGAACGAGTGTGTGATCGTTTCAAGTTGATTCCTCTACTAATTAATTGAAAAATAATGAGAATTTTGTCCAATTTTGATTTGACTGACCGGTTTTCTTTTCTCATCAAATTTCC

mRNA sequence

GAAGCTCAAAAGTCCAACACAAATATTAAAACAGAAATGGCTTCTTTCTCTTTGAAAGCCATATTTTTCTTTGCCTTTTCTGTTCTTCTCTGTTTTCTTCCAAACATGGTCAATGCAGGCATCACAAGGCACTACAATTTTCATATTCAATTGCAAAATGTAACAAGACTCTGTCGAACAAAGACCATTGTGACCGTCAACGGGCAGTTTCCCGGGCCGAGAATCATCGCTAGGGAAGGCGACCGCCTCTTGATCAAAGTGGTCAATCACGTCCAAAACAATATATCTCTCCATTGGCACGGAGTGCGACAGATGAGGAGCGGGTGGGCAGACGGGCCAGCGTACGTGACGCAATGCCCGATCCAAACAGGGCAGAGCTATGTGTATAACTTCACATTGAATGGGCAGAGAGGGACATTGTTTTGGCATGCTCATATCTCATGGCTAAGATCAACTGTGTATGGGCCAATCATCATTCTTCCCAAAACCAATCAGCCATACCCTTTTCCTCAACCTTTTGAAGAAATTCCCATTGTTTTTGGGGAGTGGTGGAAGGCTGATACTGAGGCTGTCATAAACCAAGCAATGAAAAGTGGTGGTGCTCCAAATATTTCTGATGCTTTTACCTTTAATGGCCTCCCTGGTCCCTCCTATAATTGCTCTGCTCAAGATACCTTCAAGCTCAAGGTAAAACCAGGCAAATCCTATCTTCTCCGACTGATCAACGCTGCCCTCAATGACGAGCTCTTCTTCAGCATCGCCAACCACACACTCACCGTCGTCGAGGCCGACGCCATTTACGTCAAGCCCTTCGTAACCGACGTCCTTTTGATGGCTCCAGGACAAACCATGAACGTACTACTCCAAGCCAAGGCCAACGCTCCCAACGCCACATTCCTCATCGCGGCCCGGCCCTACGCCGTCGGGCCTGCCCCGTTCGACAACACGACCGTAACCGGCCTGCTCGAATACGAACCCAAAAAATCCCTATCAAAAAACAACAACAAGCTCCCTCTCCTAAAACCGGCCCTTCCCCGGTTCAACGACTCGAGTTTTTCCATCAAATTTAACGGGAAGATTCGGAGGCTGGTGAGTTCGAGATTCCCGGCGAAAGTTCCGACGAGGGTTGACCGGAGATTCTTTTTCACGGTGGGTTTGGGGGCGCTGCCGTGCGGGAGAAACGGATCGTGTCAGGGCCCGAACAATTTGAGATTCTCGGCATCGATTAATAACGTGACGTTTGTGCAGCCAAGTACGGCTCTTTTGCAAGCTCATTTCTTTAACAAATCTAACGGCGTTTATACCACTGATTTGCCGGTCAATCCGCCGTTTAAATTTAACTACACAGGCTCGCCGCCGAAGAACTTGGTAGTAAGCAGCGGAACAAAGGTGGCGGTGGTGCCGTACAACGCGGGCGTGGAAGTAGTAATGCAGGACACGAGCATAATCACGGCGGAGAGCCACCCGCTCCACCTGCACGGCTTCAATTTCTTCGTCTTAGCCCAAGGAATCGGCAATTTCGACCCCAGCAGAGACCCGGCCAGGTTCAACCTCGCAGACCCCCCCGAGAGGAACACCGTCGGCGTCCCCTCCGGCGGCTGGGTCGCCGTCCGGTTCGTCGCCGACAATCCCGGGGCTTGGTTTATGCACTGCCACTTGGAGGTTCACACCAGTTGGGGCCTCAAAATGGCCTGGATTGTGCAGGACGGAAACCGCCCCGATCAGAAGCTCCCGCCGCCGCCCCCCGATTTGCCCAAATGCTAACTGTTTTTCTTTTTTTTCTTCCGAAACATTTCCAAACGGAAAACCAGAAAGATTTCAAGATCATCCTTTTCGTATTCAATTGAACGAGTGTGTGATCGTTTCAAGTTGATTCCTCTACTAATTAATTGAAAAATAATGAGAATTTTGTCCAATTTTGATTTGACTGACCGGTTTTCTTTTCTCATCAAATTTCC

Coding sequence (CDS)

ATGGCTTCTTTCTCTTTGAAAGCCATATTTTTCTTTGCCTTTTCTGTTCTTCTCTGTTTTCTTCCAAACATGGTCAATGCAGGCATCACAAGGCACTACAATTTTCATATTCAATTGCAAAATGTAACAAGACTCTGTCGAACAAAGACCATTGTGACCGTCAACGGGCAGTTTCCCGGGCCGAGAATCATCGCTAGGGAAGGCGACCGCCTCTTGATCAAAGTGGTCAATCACGTCCAAAACAATATATCTCTCCATTGGCACGGAGTGCGACAGATGAGGAGCGGGTGGGCAGACGGGCCAGCGTACGTGACGCAATGCCCGATCCAAACAGGGCAGAGCTATGTGTATAACTTCACATTGAATGGGCAGAGAGGGACATTGTTTTGGCATGCTCATATCTCATGGCTAAGATCAACTGTGTATGGGCCAATCATCATTCTTCCCAAAACCAATCAGCCATACCCTTTTCCTCAACCTTTTGAAGAAATTCCCATTGTTTTTGGGGAGTGGTGGAAGGCTGATACTGAGGCTGTCATAAACCAAGCAATGAAAAGTGGTGGTGCTCCAAATATTTCTGATGCTTTTACCTTTAATGGCCTCCCTGGTCCCTCCTATAATTGCTCTGCTCAAGATACCTTCAAGCTCAAGGTAAAACCAGGCAAATCCTATCTTCTCCGACTGATCAACGCTGCCCTCAATGACGAGCTCTTCTTCAGCATCGCCAACCACACACTCACCGTCGTCGAGGCCGACGCCATTTACGTCAAGCCCTTCGTAACCGACGTCCTTTTGATGGCTCCAGGACAAACCATGAACGTACTACTCCAAGCCAAGGCCAACGCTCCCAACGCCACATTCCTCATCGCGGCCCGGCCCTACGCCGTCGGGCCTGCCCCGTTCGACAACACGACCGTAACCGGCCTGCTCGAATACGAACCCAAAAAATCCCTATCAAAAAACAACAACAAGCTCCCTCTCCTAAAACCGGCCCTTCCCCGGTTCAACGACTCGAGTTTTTCCATCAAATTTAACGGGAAGATTCGGAGGCTGGTGAGTTCGAGATTCCCGGCGAAAGTTCCGACGAGGGTTGACCGGAGATTCTTTTTCACGGTGGGTTTGGGGGCGCTGCCGTGCGGGAGAAACGGATCGTGTCAGGGCCCGAACAATTTGAGATTCTCGGCATCGATTAATAACGTGACGTTTGTGCAGCCAAGTACGGCTCTTTTGCAAGCTCATTTCTTTAACAAATCTAACGGCGTTTATACCACTGATTTGCCGGTCAATCCGCCGTTTAAATTTAACTACACAGGCTCGCCGCCGAAGAACTTGGTAGTAAGCAGCGGAACAAAGGTGGCGGTGGTGCCGTACAACGCGGGCGTGGAAGTAGTAATGCAGGACACGAGCATAATCACGGCGGAGAGCCACCCGCTCCACCTGCACGGCTTCAATTTCTTCGTCTTAGCCCAAGGAATCGGCAATTTCGACCCCAGCAGAGACCCGGCCAGGTTCAACCTCGCAGACCCCCCCGAGAGGAACACCGTCGGCGTCCCCTCCGGCGGCTGGGTCGCCGTCCGGTTCGTCGCCGACAATCCCGGGGCTTGGTTTATGCACTGCCACTTGGAGGTTCACACCAGTTGGGGCCTCAAAATGGCCTGGATTGTGCAGGACGGAAACCGCCCCGATCAGAAGCTCCCGCCGCCGCCCCCCGATTTGCCCAAATGCTAA

Protein sequence

MASFSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC
Homology
BLAST of Sed0008819 vs. NCBI nr
Match: XP_022983919.1 (laccase-17-like [Cucurbita maxima])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.1e-306
Identity = 503/576 (87.33%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
           MA  SLKA F+FAFSV LLC LP+M +AGITRHY FHIQLQNVTRLC+TKT+VTVNGQFP
Sbjct: 1   MAFDSLKATFYFAFSVSLLCLLPSMADAGITRHYKFHIQLQNVTRLCQTKTVVTVNGQFP 60

Query: 61  GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
           GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQ+RSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 61  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQVRSGWADGPAYVTQCPIQTGQSYVYNF 120

Query: 121 TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
           T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 121 TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 180

Query: 181 INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
           INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 181 INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240

Query: 241 SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
           SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 241 SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 300

Query: 301 PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
            FDNTTVTGLLEYE  KSL K N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAK
Sbjct: 301 AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPILPRFNDTSFSIKFNGKIRSLANSKFPAK 360

Query: 361 VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
           VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 361 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 420

Query: 421 GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
           GVYTTD P NPPFKFNYTGSPPKN +VSSGTKVAV+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 421 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVAVLPYNAAVELVMQDTSIVTAESHPLH 480

Query: 481 LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
           LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 481 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 540

Query: 541 HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 541 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 576

BLAST of Sed0008819 vs. NCBI nr
Match: XP_038905310.1 (laccase-17-like [Benincasa hispida])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.6e-305
Identity = 499/577 (86.48%), Postives = 536/577 (92.89%), Query Frame = 0

Query: 1   MASFSLKAIFFFAF--SVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQF 60
           MA  S KAIFFFAF  ++LLC LP + NAGITRHY F IQLQNVTRLC+TKTIVTVNGQF
Sbjct: 20  MAFISQKAIFFFAFFVNILLCLLPYIANAGITRHYKFQIQLQNVTRLCQTKTIVTVNGQF 79

Query: 61  PGPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYN 120
           PGPRIIAREGDRL+IKVVNHVQNNISLHWHGVRQ+RSGWADGPAYVTQCPIQTGQSYVYN
Sbjct: 80  PGPRIIAREGDRLIIKVVNHVQNNISLHWHGVRQLRSGWADGPAYVTQCPIQTGQSYVYN 139

Query: 121 FTLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEA 180
           FT+ GQRGTLFWHAHISWLRSTVYGPIIILPK +QPYPFPQPF+E+ I+FGEWWKADTEA
Sbjct: 140 FTVKGQRGTLFWHAHISWLRSTVYGPIIILPKAHQPYPFPQPFKEVHIIFGEWWKADTEA 199

Query: 181 VINQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELF 240
           VINQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKP KSYL+RLINAALNDELF
Sbjct: 200 VINQAMQNGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPAKSYLVRLINAALNDELF 259

Query: 241 FSIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGP 300
           FSIANHTLTVVEADA+YVKPF TD +L++PGQTMNVLL  K+NAPNATFLIAARPYA  P
Sbjct: 260 FSIANHTLTVVEADAVYVKPFKTDTVLISPGQTMNVLLHTKSNAPNATFLIAARPYATAP 319

Query: 301 APFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPA 360
           A FDNTTVTGLL+YEP KSL K+NNKLPLLKP LPRFND+SFSIKFN KIR L +S+FPA
Sbjct: 320 AAFDNTTVTGLLQYEPTKSLLKSNNKLPLLKPVLPRFNDTSFSIKFNAKIRSLANSKFPA 379

Query: 361 KVPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKS 420
           K+PTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKS
Sbjct: 380 KLPTRVDRRFFFTVGLGLLPCWRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKS 439

Query: 421 NGVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPL 480
           NGVYTTD P NPP KFNYTG+PPKNL+VSSGTKVAV+PYN  VE+VMQDTSI+TAESHPL
Sbjct: 440 NGVYTTDFPANPPIKFNYTGTPPKNLMVSSGTKVAVLPYNTAVELVMQDTSIVTAESHPL 499

Query: 481 HLHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMH 540
           HLHGFNFFV+ QGIGNFDP++DPARFNLADP ERNT+GVPSGGWVA+RFVADNPGAWFMH
Sbjct: 500 HLHGFNFFVVGQGIGNFDPNKDPARFNLADPAERNTIGVPSGGWVAIRFVADNPGAWFMH 559

Query: 541 CHLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           CHLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 560 CHLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 596

BLAST of Sed0008819 vs. NCBI nr
Match: KAG7017284.1 (Laccase-17 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1058.5 bits (2736), Expect = 2.0e-305
Identity = 503/576 (87.33%), Postives = 535/576 (92.88%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
           MA  SLKA F FAFSV LLC LP+  NAGITRHY FH+QLQNVTRLC+TKT+VTVNGQFP
Sbjct: 1   MAFDSLKATFCFAFSVSLLCLLPSTANAGITRHYKFHVQLQNVTRLCQTKTVVTVNGQFP 60

Query: 61  GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
           GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 61  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120

Query: 121 TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
           T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 121 TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 180

Query: 181 INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
           INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 181 INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240

Query: 241 SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
           SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 241 SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 300

Query: 301 PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
            FDNTTVTGLLEYE  KSL K N KLPL KP LPRFNDSSFSIKFNGKIR L +S+FPAK
Sbjct: 301 AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPVLPRFNDSSFSIKFNGKIRSLANSKFPAK 360

Query: 361 VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
           VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 361 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 420

Query: 421 GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
           GVYTTD P NPPFKFNYTGSPPKN +VSSGTKV V+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 421 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLH 480

Query: 481 LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
           LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 481 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 540

Query: 541 HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 541 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 576

BLAST of Sed0008819 vs. NCBI nr
Match: XP_023528616.1 (laccase-17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.5e-305
Identity = 503/576 (87.33%), Postives = 535/576 (92.88%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
           MA  SLKA F FAFSV LLC LP+  NAGITRHY FHIQLQNVTRLC+TKT+VTVNGQFP
Sbjct: 1   MAFDSLKATFCFAFSVSLLCLLPSTANAGITRHYKFHIQLQNVTRLCQTKTVVTVNGQFP 60

Query: 61  GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
           GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 61  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120

Query: 121 TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
           T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 121 TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 180

Query: 181 INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
           INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 181 INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240

Query: 241 SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
           SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 241 SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 300

Query: 301 PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
            FDNTTVTGLLEYE  KSL K N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAK
Sbjct: 301 AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPVLPRFNDTSFSIKFNGKIRSLANSKFPAK 360

Query: 361 VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
           VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 361 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 420

Query: 421 GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
           GVYTTD P NPPFKFNYTGSPPKN +VSSGTKV V+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 421 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLH 480

Query: 481 LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
           LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 481 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 540

Query: 541 HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 541 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 576

BLAST of Sed0008819 vs. NCBI nr
Match: XP_022934632.1 (uncharacterized protein LOC111441767 [Cucurbita moschata])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.9e-305
Identity = 502/576 (87.15%), Postives = 535/576 (92.88%), Query Frame = 0

Query: 1    MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
            MA  SLKA F FAFSV LLC LP+  NAGITRHY FH+QLQNVTRLC+TKT+VTVNGQFP
Sbjct: 659  MAFDSLKATFCFAFSVSLLCLLPSTANAGITRHYKFHVQLQNVTRLCQTKTVVTVNGQFP 718

Query: 61   GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
            GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 719  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 778

Query: 121  TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
            T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 779  TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 838

Query: 181  INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
            INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 839  INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 898

Query: 241  SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
            SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 899  SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 958

Query: 301  PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
             FDNTTVTGLLEYE  KSL K N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAK
Sbjct: 959  AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPVLPRFNDTSFSIKFNGKIRSLANSQFPAK 1018

Query: 361  VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
            VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 1019 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 1078

Query: 421  GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
            GVYTTD P NPPFKFNYTGSPPKN +VSSGTKV V+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 1079 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLH 1138

Query: 481  LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
            LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 1139 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 1198

Query: 541  HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
            HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 1199 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 1234

BLAST of Sed0008819 vs. ExPASy Swiss-Prot
Match: Q9FJD5 (Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 2.9e-238
Identity = 378/556 (67.99%), Postives = 459/556 (82.55%), Query Frame = 0

Query: 28  GITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVNHVQNNISLHW 87
           GITRHY   I++QNVTRLC TK++V+VNGQFPGP++IAREGD++LIKVVN V NNISLHW
Sbjct: 22  GITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQVLIKVVNQVPNNISLHW 81

Query: 88  HGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWLRSTVYGPIII 147
           HG+RQ+RSGWADGPAY+TQCPIQTGQSYVYN+T+ GQRGTL++HAHISWLRSTVYGP+II
Sbjct: 82  HGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYHAHISWLRSTVYGPLII 141

Query: 148 LPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFTFNGLPGPSYN 207
           LPK   PYPF +P +E+P++FGEW+ ADTEA+I QA ++GG PN+SDA+T NGLPGP YN
Sbjct: 142 LPKRGVPYPFAKPHKEVPMIFGEWFNADTEAIIRQATQTGGGPNVSDAYTINGLPGPLYN 201

Query: 208 CSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVKPFVTDVLLMA 267
           CSA+DTF+L+VKPGK+YLLRLINAALNDELFFSIANHT+TVVEADAIYVKPF T+ +L+A
Sbjct: 202 CSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVKPFETETILIA 261

Query: 268 PGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKSLSKNNNK--- 327
           PGQT NVLL+ K++ P+A+F + ARPY  G   FDN+TV G+LEYEP K     +++   
Sbjct: 262 PGQTTNVLLKTKSSYPSASFFMTARPYVTGQGTFDNSTVAGILEYEPPKQTKGAHSRTSI 321

Query: 328 --LPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGALPCG-- 387
             L L KP LP  ND++F+ KF+ K+R L S  FPA VP  VDR+FFFTVGLG  PC   
Sbjct: 322 KNLQLFKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTVGLGTNPCNHK 381

Query: 388 RNGSCQGP-NNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYTGS 447
            N +CQGP N   F+ASI+N++F  P+ ALLQ+H+  +S+GVY+   P +P   FNYTG+
Sbjct: 382 NNQTCQGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWSPIVPFNYTGT 441

Query: 448 PPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDPSR 507
           PP N +VS+GT + V+PYN  VE+VMQDTSI+ AESHPLHLHGFNFFV+ QG GNFDP++
Sbjct: 442 PPNNTMVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 501

Query: 508 DPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGN 567
           DP  FNL DP ERNTVGVPSGGW A+RF+ADNPG WFMHCHLEVHTSWGL+MAW+V DG+
Sbjct: 502 DPRNFNLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWGLRMAWLVLDGD 561

Query: 568 RPDQKLPPPPPDLPKC 576
           +PDQKL PPP DLPKC
Sbjct: 562 KPDQKLLPPPADLPKC 577

BLAST of Sed0008819 vs. ExPASy Swiss-Prot
Match: Q5N9X2 (Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 3.7e-233
Identity = 377/577 (65.34%), Postives = 455/577 (78.86%), Query Frame = 0

Query: 3   SFSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPR 62
           S +L +    A S+LL  +      GITRHY F++Q+ N TRLC TK++VTVNGQ PGP 
Sbjct: 6   SSALPSPLLLAASLLLLIVQAQ---GITRHYEFNVQMANATRLCNTKSMVTVNGQCPGPE 65

Query: 63  IIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLN 122
           ++AREGDR++I+V N+V +NISLHWHGVRQ+R+GWADGPAY+TQCPIQTGQSYVYNFT+ 
Sbjct: 66  LVAREGDRVVIRVTNNVAHNISLHWHGVRQVRTGWADGPAYITQCPIQTGQSYVYNFTVA 125

Query: 123 GQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQ 182
           GQRGTL+WHAHISWLR+TVYG ++ILPK   PYPFP P +E+P++FGEWW ADTE V+NQ
Sbjct: 126 GQRGTLWWHAHISWLRATVYGALVILPKLGVPYPFPAPHKEVPVIFGEWWNADTEEVVNQ 185

Query: 183 AMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIA 242
           A+++GG PN+SDAFT NGLPGP YNCSAQDTFKLKVKPGK+Y+LRLINAALN+ELFF++A
Sbjct: 186 AVQTGGGPNVSDAFTINGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNEELFFAVA 245

Query: 243 NHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVG-PAPF 302
           NHTLTVVE DA+YVKPF  D L+++PGQT NVLL AK   P A F ++A PY+   P  F
Sbjct: 246 NHTLTVVEVDAVYVKPFTVDTLVISPGQTTNVLLTAKPYYPGANFYMSAAPYSTARPGTF 305

Query: 303 DNTTVTGLLEYE-PKKSLSKNN--NKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPA 362
            NTTV G+LEYE P  S S  +    LPL KP LP+ ND+ F   F  K+R L +  +PA
Sbjct: 306 GNTTVAGILEYENPAMSPSAASFVKGLPLFKPTLPQLNDTDFVTNFTDKLRSLATPEYPA 365

Query: 363 KVPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKS 422
            VP  VD+RFFFTVGLG LPC  N +CQGPNN + +AS+NNV+FV P+ ALLQ+HF   S
Sbjct: 366 AVPQSVDKRFFFTVGLGTLPCPANMTCQGPNNTQMAASMNNVSFVLPARALLQSHFTGLS 425

Query: 423 NGVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPL 482
           +GVY  D PV P   FNYTG+PP N  V +GTK+ V+ YN  VE+VMQDTSI+  ESHPL
Sbjct: 426 SGVYAPDFPVAPLSPFNYTGTPPNNTNVKTGTKLLVLRYNTSVELVMQDTSILGIESHPL 485

Query: 483 HLHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMH 542
           HLHGFNFFV+ QG GN+D   DPA+FNL DP ERNTVGVP+GGWVA+RF+ADNPG WFMH
Sbjct: 486 HLHGFNFFVIGQGFGNYDAVNDPAKFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMH 545

Query: 543 CHLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           CHLE HT+WGL+MAW+V DG+ P+QKL PPP DLPKC
Sbjct: 546 CHLEAHTTWGLRMAWLVLDGSHPNQKLLPPPSDLPKC 579

BLAST of Sed0008819 vs. ExPASy Swiss-Prot
Match: B9FJH4 (Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2)

HSP 1 Score: 806.2 bits (2081), Expect = 2.4e-232
Identity = 373/558 (66.85%), Postives = 438/558 (78.49%), Query Frame = 0

Query: 18  LCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVN 77
           L  L  M    ITR Y F +Q  +VTRLC TK+IVTVNGQ+PGP + AREGD + + VVN
Sbjct: 17  LMTLSAMGAEAITRQYLFDVQTTSVTRLCSTKSIVTVNGQYPGPTLFAREGDHVEVTVVN 76

Query: 78  HVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWL 137
           H   N+S+HWHG+RQ+ SGWADGP+Y+TQCPIQ G SYVY FT+ GQRGTL+WHAHISWL
Sbjct: 77  HSPYNMSIHWHGIRQLLSGWADGPSYITQCPIQPGGSYVYRFTITGQRGTLWWHAHISWL 136

Query: 138 RSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFT 197
           R+TV+GP++ILP     YPFP P EE+PI+FGEWW  DTEAVI+QA+++GG PNISDA+T
Sbjct: 137 RATVHGPMVILPPAGVGYPFPAPHEEVPIMFGEWWNNDTEAVISQALQTGGGPNISDAYT 196

Query: 198 FNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVK 257
            NGLPGP YNCSAQDTFKLKVKPGK+Y+LRLINAALNDELFFSIANHTLTVV+ DA+YVK
Sbjct: 197 LNGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNDELFFSIANHTLTVVDVDALYVK 256

Query: 258 PFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKS 317
           PF  D L++APGQT NVLL AK   P A++ + ARPY      FDNTTV G+LEY+    
Sbjct: 257 PFTVDTLIIAPGQTSNVLLTAKPTYPGASYYMLARPYTTTQGTFDNTTVAGVLEYDDPCP 316

Query: 318 LSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGAL 377
            +     +P+  P LP+ ND++    F  K+R L S+ +PA VP +VD RFFFTVGLG  
Sbjct: 317 TTAAGKIVPIFSPTLPQINDTNAVSNFTAKLRSLASAGYPAAVPQQVDHRFFFTVGLGTH 376

Query: 378 PCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYT 437
           PC  NG+CQGPN  RF+ASINNV+FV P+TALLQ+HF  KS GVY ++ P  P   FNYT
Sbjct: 377 PCAVNGTCQGPNGSRFAASINNVSFVLPATALLQSHFAGKSKGVYASNFPYYPLNPFNYT 436

Query: 438 GSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDP 497
           G+PP N  V +GTKV V+PY A VE+VMQDTSI+ AESHPLHLHGFNFFV+ QG GNFDP
Sbjct: 437 GTPPNNTNVMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP 496

Query: 498 SRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQD 557
             DPA+FNL DP ERNTVGVP+GGWVA+RF ADNPG WFMHCHLEVH SWGLKMAW+V D
Sbjct: 497 INDPAKFNLYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLVLD 556

Query: 558 GNRPDQKLPPPPPDLPKC 576
           G+RPDQKLPPPP DLPKC
Sbjct: 557 GSRPDQKLPPPPLDLPKC 574

BLAST of Sed0008819 vs. ExPASy Swiss-Prot
Match: P0DKK6 (Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 2.4e-232
Identity = 373/558 (66.85%), Postives = 438/558 (78.49%), Query Frame = 0

Query: 18  LCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVN 77
           L  L  M    ITR Y F +Q  +VTRLC TK+IVTVNGQ+PGP + AREGD + + VVN
Sbjct: 17  LMTLSAMGAEAITRQYLFDVQTTSVTRLCSTKSIVTVNGQYPGPTLFAREGDHVEVTVVN 76

Query: 78  HVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWL 137
           H   N+S+HWHG+RQ+ SGWADGP+Y+TQCPIQ G SYVY FT+ GQRGTL+WHAHISWL
Sbjct: 77  HSPYNMSIHWHGIRQLLSGWADGPSYITQCPIQPGGSYVYRFTITGQRGTLWWHAHISWL 136

Query: 138 RSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFT 197
           R+TV+GP++ILP     YPFP P EE+PI+FGEWW  DTEAVI+QA+++GG PNISDA+T
Sbjct: 137 RATVHGPMVILPPAGVGYPFPAPHEEVPIMFGEWWNNDTEAVISQALQTGGGPNISDAYT 196

Query: 198 FNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVK 257
            NGLPGP YNCSAQDTFKLKVKPGK+Y+LRLINAALNDELFFSIANHTLTVV+ DA+YVK
Sbjct: 197 LNGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALNDELFFSIANHTLTVVDVDALYVK 256

Query: 258 PFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKS 317
           PF  D L++APGQT NVLL AK   P A++ + ARPY      FDNTTV G+LEY+    
Sbjct: 257 PFTVDTLIIAPGQTSNVLLTAKPTYPGASYYMLARPYTTTQGTFDNTTVAGVLEYDDPCP 316

Query: 318 LSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGAL 377
            +     +P+  P LP+ ND++    F  K+R L S+ +PA VP +VD RFFFTVGLG  
Sbjct: 317 TTAAGKIVPIFSPTLPQINDTNAVSNFTAKLRSLASAGYPAAVPQQVDHRFFFTVGLGTH 376

Query: 378 PCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYT 437
           PC  NG+CQGPN  RF+ASINNV+FV P+TALLQ+HF  KS GVY ++ P  P   FNYT
Sbjct: 377 PCAVNGTCQGPNGSRFAASINNVSFVLPATALLQSHFAGKSKGVYASNFPYYPLNPFNYT 436

Query: 438 GSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDP 497
           G+PP N  V +GTKV V+PY A VE+VMQDTSI+ AESHPLHLHGFNFFV+ QG GNFDP
Sbjct: 437 GTPPNNTNVMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDP 496

Query: 498 SRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQD 557
             DPA+FNL DP ERNTVGVP+GGWVA+RF ADNPG WFMHCHLEVH SWGLKMAW+V D
Sbjct: 497 INDPAKFNLYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLVLD 556

Query: 558 GNRPDQKLPPPPPDLPKC 576
           G+RPDQKLPPPP DLPKC
Sbjct: 557 GSRPDQKLPPPPLDLPKC 574

BLAST of Sed0008819 vs. ExPASy Swiss-Prot
Match: Q10ND7 (Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 6.9e-232
Identity = 377/582 (64.78%), Postives = 463/582 (79.55%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSVLLCFLPNMVNAG-ITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
           M +  L  +  +   +LL  LP +  AG  TR+Y F+++LQNVTRLC T+ I TVNG+FP
Sbjct: 1   MGARCLALLLLYGTLLLLLLLPQLPLAGAATRYYTFNVKLQNVTRLCNTRAIPTVNGKFP 60

Query: 61  GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
           GP+I+ REGDR+++KVVN++++NI++HWHGVRQMR+GW+DGPAYVTQCPIQTGQSYVYNF
Sbjct: 61  GPKIVTREGDRVVVKVVNNIKDNITIHWHGVRQMRTGWSDGPAYVTQCPIQTGQSYVYNF 120

Query: 121 TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
           T+NGQRGTLFWHAH+SWLRST+YGPIIILPK   P PF +P +++PI+FGEW+ AD EA+
Sbjct: 121 TINGQRGTLFWHAHVSWLRSTLYGPIIILPKAGLPLPFTEPHKDVPIIFGEWFNADPEAI 180

Query: 181 INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
           + QA+++GG PN+SDA+T NGLPGP YNCS++DTF+LKV+PGK YLLRLINAALNDELFF
Sbjct: 181 VAQALQTGGGPNVSDAYTINGLPGPLYNCSSKDTFRLKVQPGKMYLLRLINAALNDELFF 240

Query: 241 SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAK--ANAPNATFLIAARPYAVG 300
           S+ANHTLTVV+ DA YVKPF TDV+L+ PGQT NVLL+AK  A A  AT L+ ARPYA G
Sbjct: 241 SVANHTLTVVDVDASYVKPFDTDVVLITPGQTTNVLLRAKPTAEAAGATHLMMARPYATG 300

Query: 301 -PAPFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRF 360
            P  +DNTTV  +LEY P   +      LPLL+P+LP  ND++F+  F  K+R L    +
Sbjct: 301 RPGTYDNTTVAAVLEYAPPGHI----KSLPLLRPSLPALNDTAFAAGFAAKLRSLACPDY 360

Query: 361 PAKVPTRVDRRFFFTVGLGALPC--GRNGSCQGP-NNLRFSASINNVTFVQPSTALLQAH 420
           P+ VP RVD+ FFF VGLG  PC    N +CQGP N  +F+ASINNV+F  P+TALLQAH
Sbjct: 361 PSNVPRRVDKPFFFAVGLGTTPCPGSNNQTCQGPTNTTKFTASINNVSFDMPTTALLQAH 420

Query: 421 FFNKSNGVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITA 480
           +  +S GVYT D P +P   FNYTG+PP N  VS+GT+V V+PYNA VEVV+QDTSI+ A
Sbjct: 421 YTGQSAGVYTADFPASPLEPFNYTGTPPNNTNVSNGTRVVVLPYNASVEVVLQDTSILGA 480

Query: 481 ESHPLHLHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPG 540
           ESHPLHLHGF+FFV+ QG GN+DPS+ PA FNL DP +RNTVGVP+GGWVA+RF ADNPG
Sbjct: 481 ESHPLHLHGFDFFVVGQGTGNYDPSKHPAEFNLVDPVQRNTVGVPAGGWVAIRFFADNPG 540

Query: 541 AWFMHCHLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
            WFMHCHLEVHT+WGLKMAW+V DG  P+QKL PPP DLP C
Sbjct: 541 VWFMHCHLEVHTTWGLKMAWVVNDGPLPEQKLMPPPSDLPMC 578

BLAST of Sed0008819 vs. ExPASy TrEMBL
Match: A0A6J1J8Z0 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111482397 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.4e-306
Identity = 503/576 (87.33%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
           MA  SLKA F+FAFSV LLC LP+M +AGITRHY FHIQLQNVTRLC+TKT+VTVNGQFP
Sbjct: 1   MAFDSLKATFYFAFSVSLLCLLPSMADAGITRHYKFHIQLQNVTRLCQTKTVVTVNGQFP 60

Query: 61  GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
           GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQ+RSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 61  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQVRSGWADGPAYVTQCPIQTGQSYVYNF 120

Query: 121 TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
           T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 121 TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 180

Query: 181 INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
           INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 181 INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240

Query: 241 SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
           SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 241 SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 300

Query: 301 PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
            FDNTTVTGLLEYE  KSL K N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAK
Sbjct: 301 AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPILPRFNDTSFSIKFNGKIRSLANSKFPAK 360

Query: 361 VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
           VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 361 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 420

Query: 421 GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
           GVYTTD P NPPFKFNYTGSPPKN +VSSGTKVAV+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 421 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVAVLPYNAAVELVMQDTSIVTAESHPLH 480

Query: 481 LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
           LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 481 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 540

Query: 541 HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 541 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 576

BLAST of Sed0008819 vs. ExPASy TrEMBL
Match: A0A6J1F3C7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 2.9e-305
Identity = 502/576 (87.15%), Postives = 535/576 (92.88%), Query Frame = 0

Query: 1    MASFSLKAIFFFAFSV-LLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFP 60
            MA  SLKA F FAFSV LLC LP+  NAGITRHY FH+QLQNVTRLC+TKT+VTVNGQFP
Sbjct: 659  MAFDSLKATFCFAFSVSLLCLLPSTANAGITRHYKFHVQLQNVTRLCQTKTVVTVNGQFP 718

Query: 61   GPRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 120
            GPRIIAREGDRLLIKVVN VQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF
Sbjct: 719  GPRIIAREGDRLLIKVVNRVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNF 778

Query: 121  TLNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAV 180
            T+NGQRGTLFWHAHISWLRSTVYGPIIILP+T+QPYPFP+P +E+PI+FGEWWKADTEAV
Sbjct: 779  TVNGQRGTLFWHAHISWLRSTVYGPIIILPRTHQPYPFPRPLKEVPIIFGEWWKADTEAV 838

Query: 181  INQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 240
            INQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF
Sbjct: 839  INQAMQTGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFF 898

Query: 241  SIANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPA 300
            SIA+HTLTVVEADA+YVKPF T+V+L+ PGQTMNVLL  K+NAPNATFLIAARPYA  PA
Sbjct: 899  SIADHTLTVVEADAVYVKPFKTNVVLITPGQTMNVLLHTKSNAPNATFLIAARPYATAPA 958

Query: 301  PFDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAK 360
             FDNTTVTGLLEYE  KSL K N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAK
Sbjct: 959  AFDNTTVTGLLEYESTKSLLKKNIKLPLHKPVLPRFNDTSFSIKFNGKIRSLANSQFPAK 1018

Query: 361  VPTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSN 420
            VPTRVDRRFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQAHFFNKSN
Sbjct: 1019 VPTRVDRRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQAHFFNKSN 1078

Query: 421  GVYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLH 480
            GVYTTD P NPPFKFNYTGSPPKN +VSSGTKV V+PYNA VE+VMQDTSI+TAESHPLH
Sbjct: 1079 GVYTTDFPANPPFKFNYTGSPPKNSMVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLH 1138

Query: 481  LHGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHC 540
            LHGFNFFV+ QGIGNFDP++DPA+FNLADP ERNTVGVPSGGWVA+RFVADNPGAWFMHC
Sbjct: 1139 LHGFNFFVVGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHC 1198

Query: 541  HLEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
            HLEVHTSWGLKMAWIVQDG RP+QKLPPPP DLPKC
Sbjct: 1199 HLEVHTSWGLKMAWIVQDGKRPNQKLPPPPSDLPKC 1234

BLAST of Sed0008819 vs. ExPASy TrEMBL
Match: A0A0A0LD50 (Laccase OS=Cucumis sativus OX=3659 GN=Csa_3G734120 PE=3 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.4e-296
Identity = 483/575 (84.00%), Postives = 524/575 (91.13%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPG 60
           M + S KAIF    ++LLCF+ N+VNA ITRHY F IQLQNVTRLC+TKTIVTVNGQFPG
Sbjct: 1   MVALSQKAIF---VNILLCFILNIVNARITRHYKFQIQLQNVTRLCQTKTIVTVNGQFPG 60

Query: 61  PRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFT 120
           PRIIAREGDRLLIKVVNHVQNNISLHWHGVRQ RSGWADGPAYVTQCPIQTGQSYVYNFT
Sbjct: 61  PRIIAREGDRLLIKVVNHVQNNISLHWHGVRQRRSGWADGPAYVTQCPIQTGQSYVYNFT 120

Query: 121 LNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVI 180
           ++GQRGTLFWHAHISWLRST+YGPIIILPKT+QPYPFPQPF+E+PI+FGEWWKADTE VI
Sbjct: 121 VDGQRGTLFWHAHISWLRSTLYGPIIILPKTHQPYPFPQPFKEVPIIFGEWWKADTENVI 180

Query: 181 NQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFS 240
           NQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFS
Sbjct: 181 NQAMQNGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFS 240

Query: 241 IANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAP 300
           IANHTLTVVEADA+YVKPF TDV+L+ PGQTMNVLL  K+N+PNATFLIAARPYA  P  
Sbjct: 241 IANHTLTVVEADAVYVKPFKTDVILITPGQTMNVLLHTKSNSPNATFLIAARPYATAPVA 300

Query: 301 FDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKV 360
           FDNTTVTGLLEYEP KSL   N KLPL KP LPRFND+SFSIKFNGKIR L +S+FPAKV
Sbjct: 301 FDNTTVTGLLEYEPTKSLLNKNKKLPLHKPVLPRFNDTSFSIKFNGKIRSLANSKFPAKV 360

Query: 361 PTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNG 420
           P RV  RFFFTVGLG LPC RN SCQGPNN R SASINNVTFVQP+TALLQ+HFFNKSNG
Sbjct: 361 PMRVKWRFFFTVGLGLLPCRRNRSCQGPNNTRLSASINNVTFVQPNTALLQSHFFNKSNG 420

Query: 421 VYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHL 480
           VYTTD P NPP KFNYTG+PPKN +VSSGTKV ++PYN+ VE+V+QDTSI+TAESHPLHL
Sbjct: 421 VYTTDFPANPPIKFNYTGTPPKNSMVSSGTKVVMLPYNSAVELVLQDTSIVTAESHPLHL 480

Query: 481 HGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCH 540
           HGFNFFV+ QGIGNFDP++DP +FNL DP ERNTVGVPSGGWVA+RF+ADNPGAWFMHCH
Sbjct: 481 HGFNFFVVGQGIGNFDPNKDPPKFNLVDPAERNTVGVPSGGWVAIRFIADNPGAWFMHCH 540

Query: 541 LEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           LEVHTSWGLKMAWIV+DG  P+QKLPPPP DLPKC
Sbjct: 541 LEVHTSWGLKMAWIVEDGKLPNQKLPPPPSDLPKC 572

BLAST of Sed0008819 vs. ExPASy TrEMBL
Match: A0A5D3DN43 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001580 PE=3 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 7.0e-296
Identity = 482/575 (83.83%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPG 60
           M   S KAIF    ++LLCF+ N+ NAGITRHY F IQLQNVTRLC+TKTI+TVNGQFPG
Sbjct: 1   MVFLSQKAIF---VNILLCFILNIANAGITRHYKFQIQLQNVTRLCQTKTILTVNGQFPG 60

Query: 61  PRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFT 120
           PRIIAREGDRLLIKVVNH+QNNISLHWHGV+Q RSGWADGPAYVTQCPIQTGQSYVYNFT
Sbjct: 61  PRIIAREGDRLLIKVVNHLQNNISLHWHGVQQRRSGWADGPAYVTQCPIQTGQSYVYNFT 120

Query: 121 LNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVI 180
           + GQRGTLFWHAHISWLRST+YGPI+ILPKT+QPYPFPQPF+E+PIVFGEWWKADTE VI
Sbjct: 121 VEGQRGTLFWHAHISWLRSTLYGPIVILPKTHQPYPFPQPFKEVPIVFGEWWKADTEDVI 180

Query: 181 NQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFS 240
           NQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKP K YLLRLINAALNDELFFS
Sbjct: 181 NQAMQNGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPRKPYLLRLINAALNDELFFS 240

Query: 241 IANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAP 300
           IANHTLTVVEADA+YVKPF +DV+L+ PGQTMNVLL  K+ +PNATFLIAARPYA  PA 
Sbjct: 241 IANHTLTVVEADAVYVKPFKSDVVLITPGQTMNVLLHTKSISPNATFLIAARPYATAPAA 300

Query: 301 FDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKV 360
           FDNTTVTGLLEYEP KSL   N KLPL KP LPRFND++FSIKFNGKIR L +S+FPAKV
Sbjct: 301 FDNTTVTGLLEYEPTKSLLNKNKKLPLHKPVLPRFNDTNFSIKFNGKIRSLANSKFPAKV 360

Query: 361 PTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNG 420
           P RVDRRFFFTVGLG LPC +N SCQGPNN RFSASINNVTFVQP+TALLQAHFFNKSNG
Sbjct: 361 PMRVDRRFFFTVGLGLLPCRKNRSCQGPNNTRFSASINNVTFVQPNTALLQAHFFNKSNG 420

Query: 421 VYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHL 480
           VYTTD PVNPP KFNYTG+PPKNL+VSSGTKV ++PYN  VE+V+QDTSI+TAESHPLHL
Sbjct: 421 VYTTDFPVNPPIKFNYTGTPPKNLMVSSGTKVVMLPYNTAVELVLQDTSIVTAESHPLHL 480

Query: 481 HGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCH 540
           HGFNFFV+ QGIGNFDP+RDP +FNL DP ERNTVGVPSGGWVA+RF+ADNPGAWFMHCH
Sbjct: 481 HGFNFFVVGQGIGNFDPNRDPPKFNLVDPAERNTVGVPSGGWVAIRFIADNPGAWFMHCH 540

Query: 541 LEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
            EVHTSWGLKMAWIVQDG  P+QKLPPPP DLPKC
Sbjct: 541 FEVHTSWGLKMAWIVQDGKLPNQKLPPPPSDLPKC 572

BLAST of Sed0008819 vs. ExPASy TrEMBL
Match: A0A1S3B5W8 (Laccase OS=Cucumis melo OX=3656 GN=LOC103486377 PE=3 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 7.0e-296
Identity = 482/575 (83.83%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MASFSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPG 60
           M   S KAIF    ++LLCF+ N+ NAGITRHY F IQLQNVTRLC+TKTI+TVNGQFPG
Sbjct: 1   MVFLSQKAIF---VNILLCFILNIANAGITRHYKFQIQLQNVTRLCQTKTILTVNGQFPG 60

Query: 61  PRIIAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFT 120
           PRIIAREGDRLLIKVVNH+QNNISLHWHGV+Q RSGWADGPAYVTQCPIQTGQSYVYNFT
Sbjct: 61  PRIIAREGDRLLIKVVNHLQNNISLHWHGVQQRRSGWADGPAYVTQCPIQTGQSYVYNFT 120

Query: 121 LNGQRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVI 180
           + GQRGTLFWHAHISWLRST+YGPI+ILPKT+QPYPFPQPF+E+PIVFGEWWKADTE VI
Sbjct: 121 VEGQRGTLFWHAHISWLRSTLYGPIVILPKTHQPYPFPQPFKEVPIVFGEWWKADTEDVI 180

Query: 181 NQAMKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFS 240
           NQAM++GGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKP K YLLRLINAALNDELFFS
Sbjct: 181 NQAMQNGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPRKPYLLRLINAALNDELFFS 240

Query: 241 IANHTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAP 300
           IANHTLTVVEADA+YVKPF +DV+L+ PGQTMNVLL  K+ +PNATFLIAARPYA  PA 
Sbjct: 241 IANHTLTVVEADAVYVKPFKSDVVLITPGQTMNVLLHTKSISPNATFLIAARPYATAPAA 300

Query: 301 FDNTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKV 360
           FDNTTVTGLLEYEP KSL   N KLPL KP LPRFND++FSIKFNGKIR L +S+FPAKV
Sbjct: 301 FDNTTVTGLLEYEPTKSLLNKNKKLPLHKPVLPRFNDTNFSIKFNGKIRSLANSKFPAKV 360

Query: 361 PTRVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNG 420
           P RVDRRFFFTVGLG LPC +N SCQGPNN RFSASINNVTFVQP+TALLQAHFFNKSNG
Sbjct: 361 PMRVDRRFFFTVGLGLLPCRKNRSCQGPNNTRFSASINNVTFVQPNTALLQAHFFNKSNG 420

Query: 421 VYTTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHL 480
           VYTTD PVNPP KFNYTG+PPKNL+VSSGTKV ++PYN  VE+V+QDTSI+TAESHPLHL
Sbjct: 421 VYTTDFPVNPPIKFNYTGTPPKNLMVSSGTKVVMLPYNTAVELVLQDTSIVTAESHPLHL 480

Query: 481 HGFNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCH 540
           HGFNFFV+ QGIGNFDP+RDP +FNL DP ERNTVGVPSGGWVA+RF+ADNPGAWFMHCH
Sbjct: 481 HGFNFFVVGQGIGNFDPNRDPPKFNLVDPAERNTVGVPSGGWVAIRFIADNPGAWFMHCH 540

Query: 541 LEVHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
            EVHTSWGLKMAWIVQDG  P+QKLPPPP DLPKC
Sbjct: 541 FEVHTSWGLKMAWIVQDGKLPNQKLPPPPSDLPKC 572

BLAST of Sed0008819 vs. TAIR 10
Match: AT5G60020.1 (laccase 17 )

HSP 1 Score: 825.9 bits (2132), Expect = 2.1e-239
Identity = 378/556 (67.99%), Postives = 459/556 (82.55%), Query Frame = 0

Query: 28  GITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVNHVQNNISLHW 87
           GITRHY   I++QNVTRLC TK++V+VNGQFPGP++IAREGD++LIKVVN V NNISLHW
Sbjct: 22  GITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQVLIKVVNQVPNNISLHW 81

Query: 88  HGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWLRSTVYGPIII 147
           HG+RQ+RSGWADGPAY+TQCPIQTGQSYVYN+T+ GQRGTL++HAHISWLRSTVYGP+II
Sbjct: 82  HGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYHAHISWLRSTVYGPLII 141

Query: 148 LPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFTFNGLPGPSYN 207
           LPK   PYPF +P +E+P++FGEW+ ADTEA+I QA ++GG PN+SDA+T NGLPGP YN
Sbjct: 142 LPKRGVPYPFAKPHKEVPMIFGEWFNADTEAIIRQATQTGGGPNVSDAYTINGLPGPLYN 201

Query: 208 CSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVKPFVTDVLLMA 267
           CSA+DTF+L+VKPGK+YLLRLINAALNDELFFSIANHT+TVVEADAIYVKPF T+ +L+A
Sbjct: 202 CSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVKPFETETILIA 261

Query: 268 PGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKSLSKNNNK--- 327
           PGQT NVLL+ K++ P+A+F + ARPY  G   FDN+TV G+LEYEP K     +++   
Sbjct: 262 PGQTTNVLLKTKSSYPSASFFMTARPYVTGQGTFDNSTVAGILEYEPPKQTKGAHSRTSI 321

Query: 328 --LPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGALPCG-- 387
             L L KP LP  ND++F+ KF+ K+R L S  FPA VP  VDR+FFFTVGLG  PC   
Sbjct: 322 KNLQLFKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTVGLGTNPCNHK 381

Query: 388 RNGSCQGP-NNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYTGS 447
            N +CQGP N   F+ASI+N++F  P+ ALLQ+H+  +S+GVY+   P +P   FNYTG+
Sbjct: 382 NNQTCQGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWSPIVPFNYTGT 441

Query: 448 PPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDPSR 507
           PP N +VS+GT + V+PYN  VE+VMQDTSI+ AESHPLHLHGFNFFV+ QG GNFDP++
Sbjct: 442 PPNNTMVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNK 501

Query: 508 DPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGN 567
           DP  FNL DP ERNTVGVPSGGW A+RF+ADNPG WFMHCHLEVHTSWGL+MAW+V DG+
Sbjct: 502 DPRNFNLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWGLRMAWLVLDGD 561

Query: 568 RPDQKLPPPPPDLPKC 576
           +PDQKL PPP DLPKC
Sbjct: 562 KPDQKLLPPPADLPKC 577

BLAST of Sed0008819 vs. TAIR 10
Match: AT2G29130.1 (laccase 2 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.5e-229
Identity = 360/565 (63.72%), Postives = 448/565 (79.29%), Query Frame = 0

Query: 13  AFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLL 72
           AF   + +  +  +AGITRHY F IQL+N+TRLC+TKTIVTVNG+FPGPR+ AREGD L 
Sbjct: 12  AFLFAISYNIDAASAGITRHYQFDIQLKNITRLCKTKTIVTVNGKFPGPRVTAREGDNLQ 71

Query: 73  IKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHA 132
           IKVVNHV NNIS+HWHG+RQ+RSGWADGP+YVTQCPI+ GQSYVYNFT+ GQRGTL+WHA
Sbjct: 72  IKVVNHVSNNISIHWHGIRQLRSGWADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLWWHA 131

Query: 133 HISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNI 192
           HI W+R+TVYGP+IILPK +QPYPFP+P++++PI+FGEW+ AD +AV+ QA+++G  PN 
Sbjct: 132 HIQWMRATVYGPLIILPKLHQPYPFPKPYKQVPILFGEWFNADPQAVVQQALQTGAGPNA 191

Query: 193 SDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEAD 252
           SDA TFNGLPGP YNCS +DT+KL VKPGK+YLLRLINAALNDELFF+IANHTLTVVEAD
Sbjct: 192 SDAHTFNGLPGPLYNCSTKDTYKLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEAD 251

Query: 253 AIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEY 312
           A YVKPF T+++L+ PGQT NVLL+ K   PNATF + ARPY  G    DNTTV G+L+Y
Sbjct: 252 ACYVKPFQTNIVLLGPGQTTNVLLKTKPIYPNATFYMLARPYFTGQGTIDNTTVAGILQY 311

Query: 313 EPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTV 372
           +     SKN   L ++KP+LP  N +S++  F    R L SS FPA VP  VD+++FF +
Sbjct: 312 QHHTKSSKN---LSIIKPSLPPINSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAI 371

Query: 373 GLGALPCGRNGSCQGP-NNLRFSASINNVTFVQPS-TALLQAHFFNKSNGVYTTDLPVNP 432
           GLG  PC +N +CQGP N  +F+ASINNV+F+ P+ T+LLQ++F  KS  V+ TD P  P
Sbjct: 372 GLGTNPCPKNQTCQGPTNTTKFAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAP 431

Query: 433 PFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQ 492
              FNYTG+PP N +VS GTKV V+ Y   VE+V+Q TSI+  E+HP+HLHGFNF+V+ Q
Sbjct: 432 IIPFNYTGTPPNNTMVSRGTKVVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQ 491

Query: 493 GIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLK 552
           G GNF+P+RDP  +NL DP ERNT+ +PSGGWVA+RF+ADNPG W MHCH+E+H SWGL 
Sbjct: 492 GFGNFNPARDPKHYNLVDPVERNTINIPSGGWVAIRFLADNPGVWLMHCHIEIHLSWGLT 551

Query: 553 MAWIVQDGNRPDQKLPPPPPDLPKC 576
           MAW+V DG+ P+QKL PPP D PKC
Sbjct: 552 MAWVVLDGDLPNQKLLPPPSDFPKC 573

BLAST of Sed0008819 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 682.6 bits (1760), Expect = 2.8e-196
Identity = 321/571 (56.22%), Postives = 412/571 (72.15%), Query Frame = 0

Query: 6   LKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIA 65
           +K  F F F  LL FL         + Y F +Q++N++R+C  K IVTVNG FPGP + A
Sbjct: 1   MKMGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYA 60

Query: 66  REGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQR 125
           REGDR++I V NHVQ N+S+HWHG++Q R+GWADGPAY+TQCPIQTGQSY+Y+F + GQR
Sbjct: 61  REGDRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQR 120

Query: 126 GTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMK 185
           GTL+WHAHI WLR+TVYG I+ILP   +PYPFPQP++E  I+ GEWW  D E  +NQA +
Sbjct: 121 GTLWWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNIILGEWWNKDVETAVNQANQ 180

Query: 186 SGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHT 245
            G  P +SDA T NG PGP + CS + TF ++ + GK+YLLR+INAALNDELFF IA H 
Sbjct: 181 LGAPPPMSDAHTINGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAGHN 240

Query: 246 LTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTT 305
           +TVVE DA+Y KPF T  +L+ PGQT NVL++    +PN  F+ AA P+   P   DN T
Sbjct: 241 MTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTD-RSPNRYFM-AASPFMDAPVSVDNKT 300

Query: 306 VTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVD 365
           VT +L+Y+        N  LP+L P LP  ND+SF++ +NGK++ L +  FPA VP +VD
Sbjct: 301 VTAILQYK-----GVPNTVLPIL-PKLPLPNDTSFALDYNGKLKSLNTPNFPALVPLKVD 360

Query: 366 RRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTD 425
           RR F+T+GLG   C    +C    NL  +ASINN+TF+ P TALL+AH+ N S GV+ TD
Sbjct: 361 RRLFYTIGLGINAC---PTCVNGTNL--AASINNITFIMPKTALLKAHYSNIS-GVFRTD 420

Query: 426 LPVNPPFKFNYTGSP-PKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFN 485
            P  PP  FNYTG P   NL  S+GT+++ V +N  +E+V+QDT+++T ESHP HLHG+N
Sbjct: 421 FPDRPPKAFNYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLLTVESHPFHLHGYN 480

Query: 486 FFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVH 545
           FFV+  G+GNFDP +DPA+FNL DPPERNTVGVP+GGW A+RF ADNPG WFMHCHLEVH
Sbjct: 481 FFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVH 540

Query: 546 TSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           T WGLKMA++V++G  P+  + PPP D P C
Sbjct: 541 TMWGLKMAFVVENGETPELSVLPPPKDYPSC 557

BLAST of Sed0008819 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 678.3 bits (1749), Expect = 5.3e-195
Identity = 319/547 (58.32%), Postives = 398/547 (72.76%), Query Frame = 0

Query: 29  ITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRIIAREGDRLLIKVVNHVQNNISLHWH 88
           + RHY F++ ++NVTRLC +K  VTVNG++PGP I ARE D LLIKVVNHV+ N+S+HWH
Sbjct: 25  MVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYAREDDTLLIKVVNHVKYNVSIHWH 84

Query: 89  GVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNGQRGTLFWHAHISWLRSTVYGPIIIL 148
           GVRQ+R+GWADGPAY+TQCPIQ GQ Y YN+TL GQRGTL+WHAHI WLR+TVYG ++IL
Sbjct: 85  GVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQRGTLWWHAHILWLRATVYGALVIL 144

Query: 149 PKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQAMKSGGAPNISDAFTFNGLPGPSYNC 208
           PK   PYPFP+P  E  IV GEWWK+DTE +IN+A+KSG APN+SD+   NG PGP  NC
Sbjct: 145 PKRGVPYPFPKPDNEKVIVLGEWWKSDTENIINEALKSGLAPNVSDSHMINGHPGPVRNC 204

Query: 209 SAQDTFKLKVKPGKSYLLRLINAALNDELFFSIANHTLTVVEADAIYVKPFVTDVLLMAP 268
            +Q  +KL V+ GK+YLLRL+NAALN+ELFF +A H  TVVE DA+YVKPF TD +L+AP
Sbjct: 205 PSQG-YKLSVENGKTYLLRLVNAALNEELFFKVAGHIFTVVEVDAVYVKPFKTDTVLIAP 264

Query: 269 GQTMNVLLQAKANAPNATFLIAARPYAVGPAPFDNTTVTGLLEYEPKKSLSKNNNKLPLL 328
           GQT NVLL A  +A    +L+ A P+   P   DN T T  + Y    S S     LP  
Sbjct: 265 GQTTNVLLTASKSA--GKYLVTASPFMDAPIAVDNVTATATVHYSGTLSSSPTILTLP-- 324

Query: 329 KPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPTRVDRRFFFTVGLGALPCGRNGSCQGP 388
               P  N +S +  F   +R L S ++PA VPT +D   FFTVGLG   C    +C+  
Sbjct: 325 ----PPQNATSIANNFTNSLRSLNSKKYPALVPTTIDHHLFFTVGLGLNAC---PTCKAG 384

Query: 389 NNLRFSASINNVTFVQPSTALLQAHFFNKSNGVYTTDLPVNPPFKFNYTGSPPKNLVVSS 448
           N  R  ASINNVTF+ P TALL AH+FN S GV+TTD P NPP  FNY+G    N+   +
Sbjct: 385 NGSRVVASINNVTFIMPKTALLPAHYFNTS-GVFTTDFPKNPPHVFNYSGGSVTNMATET 444

Query: 449 GTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHGFNFFVLAQGIGNFDPSRDPARFNLAD 508
           GT++  +PYNA V++V+QDT +I  E+HP+HLHGFNFF + +G+GNF+ ++DP  FNL D
Sbjct: 445 GTRLYKLPYNATVQLVLQDTGVIAPENHPVHLHGFNFFEVGRGLGNFNSTKDPKNFNLVD 504

Query: 509 PPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGNRPDQKLPPP 568
           P ERNT+GVPSGGWV +RF ADNPG WFMHCHLEVHT+WGLKMA++V++G  P+Q + PP
Sbjct: 505 PVERNTIGVPSGGWVVIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQSILPP 558

Query: 569 PPDLPKC 576
           P DLPKC
Sbjct: 565 PKDLPKC 558

BLAST of Sed0008819 vs. TAIR 10
Match: AT5G01190.1 (laccase 10 )

HSP 1 Score: 642.9 bits (1657), Expect = 2.5e-184
Identity = 315/573 (54.97%), Postives = 395/573 (68.94%), Query Frame = 0

Query: 4   FSLKAIFFFAFSVLLCFLPNMVNAGITRHYNFHIQLQNVTRLCRTKTIVTVNGQFPGPRI 63
           F ++ +  FA   LL F P  V+ G  R Y F++  + VTR+C TK IVTVNG+FPGP I
Sbjct: 3   FPIRILVLFA---LLAF-PACVH-GAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTI 62

Query: 64  IAREGDRLLIKVVNHVQNNISLHWHGVRQMRSGWADGPAYVTQCPIQTGQSYVYNFTLNG 123
            A E D +L+ VVN+V+ N+S+HWHG+RQ+R+GWADGPAY+TQCPI+ G SYVYNFT+ G
Sbjct: 63  YANEDDTILVNVVNNVKYNVSIHWHGIRQLRTGWADGPAYITQCPIKPGHSYVYNFTVTG 122

Query: 124 QRGTLFWHAHISWLRSTVYGPIIILPKTNQPYPFPQPFEEIPIVFGEWWKADTEAVINQA 183
           QRGTL+WHAH+ WLR+TV+G I+ILPK   PYPFP+P  E  I+ GEWWK+DTE V+N+A
Sbjct: 123 QRGTLWWHAHVLWLRATVHGAIVILPKLGLPYPFPKPHREEVIILGEWWKSDTETVVNEA 182

Query: 184 MKSGGAPNISDAFTFNGLPGPSYNCSAQDTFKLKVKPGKSYLLRLINAALNDELFFSIAN 243
           +KSG APN+SDA   NG PG   NC +Q  FKL V+ GK+Y+LRLINAALN+ELFF IA 
Sbjct: 183 LKSGLAPNVSDAHVINGHPGFVPNCPSQGNFKLAVESGKTYMLRLINAALNEELFFKIAG 242

Query: 244 HTLTVVEADAIYVKPFVTDVLLMAPGQTMNVLLQAKANAPNATFLIAARPYA-VGPAPFD 303
           H  TVVE DA+YVKPF TD +L+APGQT   L+ A    P+  +LIAA P+        D
Sbjct: 243 HRFTVVEVDAVYVKPFNTDTILIAPGQTTTALVSAA--RPSGQYLIAAAPFQDSAVVAVD 302

Query: 304 NTTVTGLLEYEPKKSLSKNNNKLPLLKPALPRFNDSSFSIKFNGKIRRLVSSRFPAKVPT 363
           N T T  + Y    S +      P      P  N +S +  F   +R L S  +PA VP 
Sbjct: 303 NRTATATVHYSGTLSATPTKTTSP------PPQNATSVANTFVNSLRSLNSKTYPANVPI 362

Query: 364 RVDRRFFFTVGLGALPCGRNGSCQGPNNLRFSASINNVTFVQPSTALLQAHFFNKSNGVY 423
            VD    FTVGLG   C    SC+  N  R  A+INN+TF  P TALLQAH+FN + G+Y
Sbjct: 363 TVDHDLLFTVGLGINRC---HSCKAGNFSRVVAAINNITFKMPKTALLQAHYFNLT-GIY 422

Query: 424 TTDLPVNPPFKFNYTGSPPKNLVVSSGTKVAVVPYNAGVEVVMQDTSIITAESHPLHLHG 483
           TTD P  P   F++TG PP NL     TK+  +PYN+ V+VV+QDT  +  E+HP+HLHG
Sbjct: 423 TTDFPAKPRRVFDFTGKPPSNLATMKATKLYKLPYNSTVQVVLQDTGNVAPENHPIHLHG 482

Query: 484 FNFFVLAQGIGNFDPSRDPARFNLADPPERNTVGVPSGGWVAVRFVADNPGAWFMHCHLE 543
           FNFFV+  G GN++  +D  +FNL DP ERNTVGVPSGGW A+RF ADNPG WFMHCHLE
Sbjct: 483 FNFFVVGLGTGNYNSKKDSNKFNLVDPVERNTVGVPSGGWAAIRFRADNPGVWFMHCHLE 542

Query: 544 VHTSWGLKMAWIVQDGNRPDQKLPPPPPDLPKC 576
           VHT+WGLKMA++V++G  P+Q + PPP DLPKC
Sbjct: 543 VHTTWGLKMAFLVENGKGPNQSIRPPPSDLPKC 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022983919.19.1e-30687.33laccase-17-like [Cucurbita maxima][more]
XP_038905310.11.6e-30586.48laccase-17-like [Benincasa hispida][more]
KAG7017284.12.0e-30587.33Laccase-17 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023528616.13.5e-30587.33laccase-17-like [Cucurbita pepo subsp. pepo][more]
XP_022934632.15.9e-30587.15uncharacterized protein LOC111441767 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FJD52.9e-23867.99Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1[more]
Q5N9X23.7e-23365.34Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1[more]
B9FJH42.4e-23266.85Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2[more]
P0DKK62.4e-23266.85Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1[more]
Q10ND76.9e-23264.78Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J8Z04.4e-30687.33Laccase OS=Cucurbita maxima OX=3661 GN=LOC111482397 PE=3 SV=1[more]
A0A6J1F3C72.9e-30587.15Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1[more]
A0A0A0LD502.4e-29684.00Laccase OS=Cucumis sativus OX=3659 GN=Csa_3G734120 PE=3 SV=1[more]
A0A5D3DN437.0e-29683.83Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001580 PE=3 ... [more]
A0A1S3B5W87.0e-29683.83Laccase OS=Cucumis melo OX=3656 GN=LOC103486377 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60020.12.1e-23967.99laccase 17 [more]
AT2G29130.11.5e-22963.72laccase 2 [more]
AT5G03260.12.8e-19656.22laccase 11 [more]
AT2G38080.15.3e-19558.32Laccase/Diphenol oxidase family protein [more]
AT5G01190.12.5e-18454.97laccase 10 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 162..353
e-value: 1.1E-39
score: 138.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 384..575
e-value: 6.8E-60
score: 204.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 14..149
e-value: 2.2E-36
score: 127.0
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 357..575
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 21..182
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 157..346
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 427..558
e-value: 1.2E-38
score: 131.9
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 30..575
e-value: 6.0E-269
score: 891.3
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 37..150
e-value: 4.3E-41
score: 139.7
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 163..314
e-value: 5.3E-41
score: 140.4
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 10..575
NoneNo IPR availablePANTHERPTHR11709:SF342LACCASE-10coord: 10..575
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 533..553
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 538..549
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 164..312
e-value: 2.42109E-87
score: 265.231
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 33..149
e-value: 7.33844E-74
score: 229.068
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 421..558
e-value: 2.24839E-83
score: 254.491

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0008819.1Sed0008819.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity