Sed0007639 (gene) Chayote v1

Overview


Name	Sed0007639
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Procollagen-proline 4-dioxygenase
Location	LG04: 33083917 .. 33087783 (-)
RNA-Seq Expression	Sed0007639
Synteny	Sed0007639

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAATTTGGCAATCTGTTATTCATCTTCTTCGTTTCGATCGCATCGGTTTTTCGAGAATCCAAAAGTTCATATGCTGGTTCGGCTGGCTCCACCGTAGATCCAAGTAAAGTGAAGCAGATTTCATGGAAACCGAGGTTTGTACCGCAATCGATTTCAAGCAAATGCACTATCATTTTCGTTCTATTGCGTTTGTTTGCTGAGAAAGTTGCGAAACTGAAAGAATCGAACTCTCTCGATTGTTTTCGCGATCGTCTAGGTTTAGTCCAATAACTGCTCGCCTGATAGCAAATTAGGCATACAGTTTTTGTTCTTGAAATTTTTGATGAGATGATTTCACTGATGTTGCAGAGCTTTTGTCTACGAAGGTTTTCTCACGGATCTAGAATGCGAGCGTCTGATTTCTTTAGTAAGTTTAGGTTTAGTGATATTCATGTGGAAGAGATGGCGTTTTTGTGAATCTTTGCTTTTGTTGTGCGATAGGCGAGATCGGAGCTAAGGAGATCTGAGGTTGCTGATAATGAGTCTGGAGAAGGCATGCTCAGTACTGTTCGAACCAGCTCTGGAATGTTCATTCCCAAGAGCAAGGTTAGGTTTGATTTTCATTACAAACTTATCTTCCATTTTTTCTTGATTTTTGTTTTTGATTGGAAATAAACTTCTGTTAGTAGCTATTTGATGAACTAAAAACTAAATGGAAGAAACTTGGATGTTGATTTATGAATTCGAATCTCTCACTCGTCGTGATTGTTGCTGAACTGAAAAAACAATTATAGGAGTGTTCCTCAAAGCATAAATCAGTAATCAATCTTGCTTCTTCTGCTTACCTTTTGATGTTAGTTTGATTCACAAGGTCCATTATGTGGATTACTAAGACAAAATAGAGACAAAACTATAACTATTTCATTTTTTGTTTTTAACTTTTTTAAAAACAAGTTTTTTTATAACCAATTTTAATTTTTGTTTTTAAAAATTTAAAACCTTTTTAAAATGTTAAAGGGATTTTAAAAACTAAAAAAAGTAGATTTTTAAAATCGAAAACGATGTAGATTTTAAAAAGTGATAAAACACACTACAAATAAAGTTGGAATCCACAAATCGGAGGTTTATTGAATCAATAATATTATAGAATAATTTTATTTTATTTTTCTAAATATTAAAAATAATAATTTTTTATTTTTTTAAAAACCAAAATCCATTTTCGAACGTATTTTTAGTTTTTAGTATCTAAAAACAAAACAAAAAATGATTATCAAATATGTATGATTTTTATTTTTAAAAACCAAAAACTAAAAATCAAAAACGAAAAAAAAAAATTATCAAATTGGTCTAGGTTTTTTGTTCTTGCCTTTTTTCTTTTTTAGGAAACGAAGTTGACTTTGATAAAATAAAAAAGTTACACAGGCGGAAAAGAGAGCACAAGCTTGCAGGAATGAAAACTTAAAATCTCATTCTCCATTATTGTTAAATTAGGACTAGATTAAAAAGCTTGAGCAGTTAGGTATAATAAATCTAACAGATTGTCTAGTAGGAAAATTTTTAGATAACATGTAGATCCATCTCGAAGCCAACTGATATTGATAATAAGTGTAGTAGTTCACTTATCTTATAAAGGTCGAACAACCTCCTTACCTTATTAACGTGAATTCTAAAAAAATATTGTCTATACCCGAGTGTACTTTGTGGTGTTCTTCCCAATCGTGTCTTAAAATTAATTTATGGTATGCCAATAAATATTTACTTTCTTTCTATTGGGTATCACTAGTTGAGATCTCTTATTACCCCATGTGTTTGTTTTGGACATTTACATTTTTATTTGATATTTAGGATCCTATTGTTAGTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCTAAAGGTACACGTTCTGTTCACAGTCAAATAACATATTTCACCATTAACACGTTTTGTTTTATATATTTGAATCACTGGCCGATTTTCCATAAGTAACATTCATGATGGGGAAAATGATAAATGTTATTTGTATTTCTGGTTTTCACAGAGAATGGAGAAGACATTCAGGTATTAAGGTATGAGCATGGGCAGAAATATGAATCACATTATGATTACTTTGTTGACAAAGTGAACATTGCCCGGGGAGGACATCGTTTGGCTACTGTCCTCATGTATCTATCCGATGTGACCAAAGGCGGCGAAACGGTTTTCCCATTGGCAGAGGTTAGTTCGAATGTCGATTTCAGAAACTAACTACTTTACTAGCTAGAACTACACCATTATATTTCTCCCTTGCAATTGTAAGATTACCTCTTCTAAGACTTCACTACTTATGAACCTTGGTGCATAGTCTTCTGATGTAAAACTTACTTTCCACAGCTTAGTAGTTATTTAAACAACTCTTTATGTTTGATCCCACCTCTTTTTTAACATAGTTTGTATATATATATATCCCAGTACTTTCATTTACTTGGCTGTAATTCCACTATAAAAATTTACATCCTAACGAGCTATTATTATAACTTTTCATTTAATATCTCAAAATAACTTCTCATGCAACTACTCATCCAAACGACCCCTTAGGGTTATTGAAATTCTTCATCTCAGAAATAAATTGGCATTTAAGTTACATCACATTTAGATTGGAAATGAATCTGGTTAAGAGAGATGGCTCTAATCAACGGAAGATCTGATTGAACTTGTTATATAACCTCTGTGAGCAGAAATCTCCCCATCGGATGGCATCCGAAACCGACGAGGATCTCTCCGAGTGTGCAAGGAAAGGAATTGCAGGTGAGTTTGTTATAACACAAATTAATTGCTTCTTTCGTATTTATTAAACTAAATCATCGTAAATTGACCTAATCGTCAAATAAGAGTTATGGAATTAATCTATAATGATTACTTATCTAAGAATTAATTTCGTACGTGTTTCTTCGACAATTAATTGAATAGTCGAAACATGCATAAGCTGGTCGGAACATTAAAGTCATGTTTGATAACCATTTCGATATTTAAAAAATAGATTCGTTTGATAATCAATTTTGGTTGTTGTTTTTAAAATTTTAAAAAAGTTTTTAAAATATTAAAGAAATTCTAAAAGCTAAAAAAAGTAGCTTTTAAAAAAAGATTTTTATTTTTGTTTTTAAAAAAGCATGAATATATCAAGAATAAAGAGTAAAAAACTAAAAAAATCAAAACAAAATGTGATGTTTGAAAACAAAACCCAGTTATCGAACATGTTTTTTGGTTTTTAATTTTTAAAAACATAAAACTAAAATAAGTTATCAAACATATACAGTTTTTATTTTTTAAAACAAAAAACCAATCCCAAGGGGTGGCACAATAGTTGAAAACTTGGGCTTTAAAGGTATACTCGCCTCAAGGTCCTAGGTTTAAAATTCAGTTGTGACATTACTCCTTCAATGTCTTCCGGTGCCTGACCTATGGACGGGCGTGGTTACCCTTGTTTCAAAAAAAAAAAACAAAAAACGAAATGGTTATTAAACGGGACTTAAACTATAGAACTCGCACGAAATCAATTTGCTTTTAATGTGACTTGATTTACCCTTGGAGAACTGTGCAGTGAAACCAAAGAAAGGGGATGCGCTACTTTTCTTTAGTCTTGAACCAAACGCTATCCCAGACAGCAACAGTCTGCATGGAGGTTGCCCTGTTCTTGAAGGAGAAAAATGGTCGGCAACAAAGTGGATTCACGTGGACTCTTTCACCAAAAACCTAGCCAACATTGGGACCTGTACTGATCTAAATGAGAGCTGTGAAAGATGGGCTGCCTTAGGCGAATGCACCAAGAACCCGGAGTATATGGTCGGGTCTCCCGAACTTCCCGGCTACTGTAGGAGCAGTTGCAGGATCTGTTGA

mRNA sequence

ATGTGGAAATTTGGCAATCTGTTATTCATCTTCTTCGTTTCGATCGCATCGGTTTTTCGAGAATCCAAAAGTTCATATGCTGGTTCGGCTGGCTCCACCGTAGATCCAAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTCTACGAAGGTTTTCTCACGGATCTAGAATGCGAGCGTCTGATTTCTTTAGCGAGATCGGAGCTAAGGAGATCTGAGGTTGCTGATAATGAGTCTGGAGAAGGCATGCTCAGTACTGTTCGAACCAGCTCTGGAATGTTCATTCCCAAGAGCAAGGATCCTATTGTTAGTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCTAAAGAGAATGGAGAAGACATTCAGGTATTAAGGTATGAGCATGGGCAGAAATATGAATCACATTATGATTACTTTGTTGACAAAGTGAACATTGCCCGGGGAGGACATCGTTTGGCTACTGTCCTCATGTATCTATCCGATGTGACCAAAGGCGGCGAAACGGTTTTCCCATTGGCAGAGAAATCTCCCCATCGGATGGCATCCGAAACCGACGAGGATCTCTCCGAGTGTGCAAGGAAAGGAATTGCAGTGAAACCAAAGAAAGGGGATGCGCTACTTTTCTTTAGTCTTGAACCAAACGCTATCCCAGACAGCAACAGTCTGCATGGAGGTTGCCCTGTTCTTGAAGGAGAAAAATGGTCGGCAACAAAGTGGATTCACGTGGACTCTTTCACCAAAAACCTAGCCAACATTGGGACCTGTACTGATCTAAATGAGAGCTGTGAAAGATGGGCTGCCTTAGGCGAATGCACCAAGAACCCGGAGTATATGGTCGGGTCTCCCGAACTTCCCGGCTACTGTAGGAGCAGTTGCAGGATCTGTTGA

Coding sequence (CDS)

ATGTGGAAATTTGGCAATCTGTTATTCATCTTCTTCGTTTCGATCGCATCGGTTTTTCGAGAATCCAAAAGTTCATATGCTGGTTCGGCTGGCTCCACCGTAGATCCAAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTCTACGAAGGTTTTCTCACGGATCTAGAATGCGAGCGTCTGATTTCTTTAGCGAGATCGGAGCTAAGGAGATCTGAGGTTGCTGATAATGAGTCTGGAGAAGGCATGCTCAGTACTGTTCGAACCAGCTCTGGAATGTTCATTCCCAAGAGCAAGGATCCTATTGTTAGTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCTAAAGAGAATGGAGAAGACATTCAGGTATTAAGGTATGAGCATGGGCAGAAATATGAATCACATTATGATTACTTTGTTGACAAAGTGAACATTGCCCGGGGAGGACATCGTTTGGCTACTGTCCTCATGTATCTATCCGATGTGACCAAAGGCGGCGAAACGGTTTTCCCATTGGCAGAGAAATCTCCCCATCGGATGGCATCCGAAACCGACGAGGATCTCTCCGAGTGTGCAAGGAAAGGAATTGCAGTGAAACCAAAGAAAGGGGATGCGCTACTTTTCTTTAGTCTTGAACCAAACGCTATCCCAGACAGCAACAGTCTGCATGGAGGTTGCCCTGTTCTTGAAGGAGAAAAATGGTCGGCAACAAAGTGGATTCACGTGGACTCTTTCACCAAAAACCTAGCCAACATTGGGACCTGTACTGATCTAAATGAGAGCTGTGAAAGATGGGCTGCCTTAGGCGAATGCACCAAGAACCCGGAGTATATGGTCGGGTCTCCCGAACTTCCCGGCTACTGTAGGAGCAGTTGCAGGATCTGTTGA

Protein sequence

MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC

Homology

[Close]

BLAST of Sed0007639 vs. NCBI nr
Match: KAG6575033.1 (putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560.8 bits (1444), Expect = 6.9e-156
Identity = 269/300 (89.67%), Postives = 283/300 (94.33%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDL+ECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLTECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG CTDLNESCERWAALGECTKNPEYMVGSPELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. NCBI nr
Match: XP_022959148.1 (probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable prolyl 4-hydroxylase 4 [Cucurbita moschata])

HSP 1 Score: 560.5 bits (1443), Expect = 9.0e-156
Identity = 269/300 (89.67%), Postives = 283/300 (94.33%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG C+DLNESCERWAALGECTKNPEYMVGSPELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. NCBI nr
Match: XP_023547984.1 (probable prolyl 4-hydroxylase 4 [Cucurbita pepo subsp. pepo] >XP_023547985.1 probable prolyl 4-hydroxylase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 7.7e-155
Identity = 268/300 (89.33%), Postives = 282/300 (94.00%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M+KF NLLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKD IVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDSIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG CTDLNESCERWAALGECTKNPEYMVGS ELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. NCBI nr
Match: XP_023000081.1 (probable prolyl 4-hydroxylase 4 [Cucurbita maxima] >XP_023000082.1 probable prolyl 4-hydroxylase 4 [Cucurbita maxima])

HSP 1 Score: 556.2 bits (1432), Expect = 1.7e-154
Identity = 269/300 (89.67%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF +LLFIF +SIASV RES  S A SA +TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRSLLFIFLISIASVVRESICSSARSASTTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + LIS+ARSEL+RSEVADNESG+  LSTVRTSSGMFIPKSKD IVSGIEDKIAAWTFLPK
Sbjct: 61  DHLISIARSELKRSEVADNESGKSKLSTVRTSSGMFIPKSKDAIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQ+YESHYDYFVDKVNIA GGHRLATVLMYLSDVTKGGETVFP+AEK
Sbjct: 121 ENGEDIQVLRYEHGQRYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPMAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SPHR ASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPD+ SLHGGCPVLEGEKWS
Sbjct: 181 SPHRRASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTKSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNLAN+G CTDLNESCERWAALGECTKNPEYMVGSPELPGYCR SCR C
Sbjct: 241 ATKWIHVDSFSKNLANVGNCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRTC 300

[Close]

BLAST of Sed0007639 vs. NCBI nr
Match: XP_023006272.1 (probable prolyl 4-hydroxylase 4 [Cucurbita maxima] >XP_023006273.1 probable prolyl 4-hydroxylase 4 [Cucurbita maxima])

HSP 1 Score: 555.8 bits (1431), Expect = 2.2e-154
Identity = 268/300 (89.33%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF  LLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRYLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG CTDLNESCERWAALGECTKNPEYMVGS ELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 2.9e-125
Identity = 211/293 (72.01%), Postives = 245/293 (83.62%), Query Frame = 0


Query: 8   LFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLA 67
           L I F +I SV  +S +S   S+   V+PSKVKQ+S KPRAFVYEGFLT+LEC+ ++SLA
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 68  RSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQ 127
           ++ L+RS VADN+SGE   S VRTSSG FI K KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 128 VLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMAS 187
           VLRYEHGQKY++H+DYF DKVNI RGGHR+AT+LMYLS+VTKGGETVFP AE    R+ S
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 188 ETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHV 247
           E  EDLS+CA++GIAVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 248 DSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           DSF + +   G CTD+NESCERWA LGECTKNPEYMVG+ ELPGYCR SC+ C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.6e-123
Identity = 207/291 (71.13%), Postives = 244/291 (83.85%), Query Frame = 0


Query: 10  IFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARS 69
           + FV+I  V  +S +    S  S ++PSKVKQ+S KPRAFVYEGFLTDLEC+ LISLA+ 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 70  ELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVL 129
            L+RS VADN++GE  +S VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 130 RYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASET 189
           RYEHGQKY++H+DYF DKVNIARGGHR+ATVL+YLS+VTKGGETVFP A++   R  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 190 DEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDS 249
            +DLS+CA+KGIAVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 250 FTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           F K L + G CTD+NESCERWA LGEC KNPEYMVG+PE+PG CR SC+ C
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 3.4e-97
Identity = 163/266 (61.28%), Postives = 203/266 (76.32%), Query Frame = 0


Query: 35  DPSKVKQISWKPRAFVYEGFLTDLECERLISLARSELRRSEVADNESGEGMLSTVRTSSG 94
           DP++V Q+SW PR F+YEGFL+D EC+  I LA+ +L +S VADN+SGE + S VRTSSG
Sbjct: 52  DPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSG 111

Query: 95  MFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGG 154
           MF+ K +D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ N+  GG
Sbjct: 112 MFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGG 171

Query: 155 HRLATVLMYLSDVTKGGETVFPLAEKSPHRMASETDEDLSECARKGIAVKPKKGDALLFF 214
           HR+ATVLMYLS+V KGGETVFP+ +    ++    D+  +ECA++G AVKP+KGDALLFF
Sbjct: 172 HRIATVLMYLSNVEKGGETVFPMWKGKATQL---KDDSWTECAKQGYAVKPRKGDALLFF 231

Query: 215 SLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDSFTKNLANIGTCTDLNESCERWAALG 274
           +L PNA  DSNSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE+WA  G
Sbjct: 232 NLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAG 291

Query: 275 ECTKNPEYMVGSPELPGYCRSSCRIC 301
           EC KNP YMVGS +  GYCR SC+ C
Sbjct: 292 ECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.2e-89
Identity = 163/291 (56.01%), Postives = 208/291 (71.48%), Query Frame = 0


Query: 11  FFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARSE 70
           F +S+  +F     S   S   +VDP+++ Q+SW PRAF+Y+GFL+D EC+ LI LA+ +
Sbjct: 9   FSLSLLLIF-----SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGK 68

Query: 71  LRRS-EVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVL 130
           L +S  VAD +SGE   S VRTSSGMF+ K +D IV+ +E K+AAWTFLP+ENGE +Q+L
Sbjct: 69  LEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQIL 128

Query: 131 RYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASET 190
            YE+GQKY+ H+DYF DK  +  GGHR+ATVLMYLS+VTKGGETVFP  +    ++    
Sbjct: 129 HYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQL---K 188

Query: 191 DEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDS 250
           D+  S+CA++G AVKP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV S
Sbjct: 189 DDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRS 248

Query: 251 FTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           F K       C D +ESC+ WA  GEC KNP YMVGS    G+CR SC+ C
Sbjct: 249 FGKKKL---VCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-64
Identity = 121/234 (51.71%), Postives = 163/234 (69.66%), Query Frame = 0


Query: 17  SVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARSELRRSEV 76
           S FR + +  +   G   D    + +SW+PRAFVY  FL+  ECE LISLA+  + +S V
Sbjct: 55  SYFRRAATERSEGLGKRGD-QWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTV 114

Query: 77  ADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQK 136
            D+E+G+   S VRTSSG F+ + +D I+  IE +IA +TF+P ++GE +QVL YE GQK
Sbjct: 115 VDSETGKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQK 174

Query: 137 YESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASETDEDLSEC 196
           YE HYDYFVD+ N   GG R+AT+LMYLSDV +GGETVFP A  + +  +     +LSEC
Sbjct: 175 YEPHYDYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAA--NMNFSSVPWYNELSEC 234

Query: 197 ARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDSF 251
            +KG++VKP+ GDALLF+S+ P+A  D  SLHGGCPV+ G KWS+TKW+HV  +
Sbjct: 235 GKKGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of Sed0007639 vs. ExPASy TrEMBL
Match: A0A6J1H545 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.4e-156
Identity = 269/300 (89.67%), Postives = 283/300 (94.33%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG C+DLNESCERWAALGECTKNPEYMVGSPELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. ExPASy TrEMBL
Match: A0A6J1KCK1 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111494385 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 8.3e-155
Identity = 269/300 (89.67%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF +LLFIF +SIASV RES  S A SA +TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRSLLFIFLISIASVVRESICSSARSASTTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + LIS+ARSEL+RSEVADNESG+  LSTVRTSSGMFIPKSKD IVSGIEDKIAAWTFLPK
Sbjct: 61  DHLISIARSELKRSEVADNESGKSKLSTVRTSSGMFIPKSKDAIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQ+YESHYDYFVDKVNIA GGHRLATVLMYLSDVTKGGETVFP+AEK
Sbjct: 121 ENGEDIQVLRYEHGQRYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPMAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SPHR ASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPD+ SLHGGCPVLEGEKWS
Sbjct: 181 SPHRRASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTKSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNLAN+G CTDLNESCERWAALGECTKNPEYMVGSPELPGYCR SCR C
Sbjct: 241 ATKWIHVDSFSKNLANVGNCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRTC 300

[Close]

BLAST of Sed0007639 vs. ExPASy TrEMBL
Match: A0A6J1L4G1 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 268/300 (89.33%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF  LLF+F + I+SV RES  SYAGSA STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRYLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI KSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SP R ASETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL NIG CTDLNESCERWAALGECTKNPEYMVGS ELPGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. ExPASy TrEMBL
Match: A0A6J1EQM4 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111436810 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.1e-154
Identity = 266/300 (88.67%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M +F ++LFIF +SIASV RES  S A SA +TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSRFRSMLFIFLISIASVVRESICSPARSASTTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + LIS+ARSEL+RSEVADNESG+  LSTVRTSSGMFIPKSKD IVSGIEDKIAAWTFLPK
Sbjct: 61  DHLISIARSELKRSEVADNESGKSKLSTVRTSSGMFIPKSKDAIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQ+YESHYDYFVDKVNIA GGHRLATVLMYLSDVTKGGETVFP+AEK
Sbjct: 121 ENGEDIQVLRYEHGQRYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPMAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           SPHR ASETDEDLS+CARKGIAVKPKKGDALLFFSLEPNAIPD+ SLHGGCPVLEGEKWS
Sbjct: 181 SPHRRASETDEDLSDCARKGIAVKPKKGDALLFFSLEPNAIPDTKSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNLAN+G CTDLNESCERWAALGECTKNPEYMVGSPELPGYCR SCR C
Sbjct: 241 ATKWIHVDSFSKNLANVGNCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRTC 300

[Close]

BLAST of Sed0007639 vs. ExPASy TrEMBL
Match: A0A1S3C816 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.3e-152
Identity = 261/300 (87.00%), Postives = 280/300 (93.33%), Query Frame = 0


Query: 1   MWKFGNLLFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M+KF NLLF F + I+S  RES  SYAGSA +TVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  ERLISLARSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPK 120
           + L+S+ARSEL+RSEVADN+SG+  LSTVRTSSGMFI K+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SPHRMASETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWS 240
           S HR A ETDEDLSECA+KGIAVKPKKGDALLFFSLEPNAIPD+NSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 300
           ATKWIHVDSF+KNL +IG CTDLNESCERWAALGECTKNPEYMVGSPE+PGYCR SCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of Sed0007639 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.1e-126
Identity = 211/293 (72.01%), Postives = 245/293 (83.62%), Query Frame = 0


Query: 8   LFIFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLA 67
           L I F +I SV  +S +S   S+   V+PSKVKQ+S KPRAFVYEGFLT+LEC+ ++SLA
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 68  RSELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQ 127
           ++ L+RS VADN+SGE   S VRTSSG FI K KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 128 VLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMAS 187
           VLRYEHGQKY++H+DYF DKVNI RGGHR+AT+LMYLS+VTKGGETVFP AE    R+ S
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 188 ETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHV 247
           E  EDLS+CA++GIAVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 248 DSFTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           DSF + +   G CTD+NESCERWA LGECTKNPEYMVG+ ELPGYCR SC+ C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Sed0007639 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-124
Identity = 207/291 (71.13%), Postives = 244/291 (83.85%), Query Frame = 0


Query: 10  IFFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARS 69
           + FV+I  V  +S +    S  S ++PSKVKQ+S KPRAFVYEGFLTDLEC+ LISLA+ 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 70  ELRRSEVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVL 129
            L+RS VADN++GE  +S VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 130 RYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASET 189
           RYEHGQKY++H+DYF DKVNIARGGHR+ATVL+YLS+VTKGGETVFP A++   R  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 190 DEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDS 249
            +DLS+CA+KGIAVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 250 FTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           F K L + G CTD+NESCERWA LGEC KNPEYMVG+PE+PG CR SC+ C
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Sed0007639 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 356.3 bits (913), Expect = 2.4e-98
Identity = 163/266 (61.28%), Postives = 203/266 (76.32%), Query Frame = 0


Query: 35  DPSKVKQISWKPRAFVYEGFLTDLECERLISLARSELRRSEVADNESGEGMLSTVRTSSG 94
           DP++V Q+SW PR F+YEGFL+D EC+  I LA+ +L +S VADN+SGE + S VRTSSG
Sbjct: 52  DPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSG 111

Query: 95  MFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGG 154
           MF+ K +D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ N+  GG
Sbjct: 112 MFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGG 171

Query: 155 HRLATVLMYLSDVTKGGETVFPLAEKSPHRMASETDEDLSECARKGIAVKPKKGDALLFF 214
           HR+ATVLMYLS+V KGGETVFP+ +    ++    D+  +ECA++G AVKP+KGDALLFF
Sbjct: 172 HRIATVLMYLSNVEKGGETVFPMWKGKATQL---KDDSWTECAKQGYAVKPRKGDALLFF 231

Query: 215 SLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDSFTKNLANIGTCTDLNESCERWAALG 274
           +L PNA  DSNSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE+WA  G
Sbjct: 232 NLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAG 291

Query: 275 ECTKNPEYMVGSPELPGYCRSSCRIC 301
           EC KNP YMVGS +  GYCR SC+ C
Sbjct: 292 ECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Sed0007639 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 334.0 bits (855), Expect = 1.3e-91
Identity = 158/274 (57.66%), Postives = 197/274 (71.90%), Query Frame = 0


Query: 35  DPSKVKQISWKPRAFVYEGFLTDLECERLISLARSELRRSEVADNESGEGM-----LSTV 94
           DP++V Q+SW PR F+YEGFL+D EC+  I LA+ +L +S VADN+SGE +     +S V
Sbjct: 52  DPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVV 111

Query: 95  RTSSGMFIPKSK---DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVD 154
           R SS           D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H+DYF D
Sbjct: 112 RQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHD 171

Query: 155 KVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASETDEDLSECARKGIAVKPK 214
           + N+  GGHR+ATVLMYLS+V KGGETVFP+ +    ++    D+  +ECA++G AVKP+
Sbjct: 172 QANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQL---KDDSWTECAKQGYAVKPR 231

Query: 215 KGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDSFTKNLANIGTCTDLNES 274
           KGDALLFF+L PNA  DSNSLHG CPV+EGEKWSAT+WIHV SF +       C D N S
Sbjct: 232 KGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVS 291

Query: 275 CERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           CE+WA  GEC KNP YMVGS +  GYCR SC+ C
Sbjct: 292 CEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Sed0007639 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 331.3 bits (848), Expect = 8.3e-91
Identity = 163/291 (56.01%), Postives = 208/291 (71.48%), Query Frame = 0


Query: 11  FFVSIASVFRESKSSYAGSAGSTVDPSKVKQISWKPRAFVYEGFLTDLECERLISLARSE 70
           F +S+  +F     S   S   +VDP+++ Q+SW PRAF+Y+GFL+D EC+ LI LA+ +
Sbjct: 9   FSLSLLLIF-----SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGK 68

Query: 71  LRRS-EVADNESGEGMLSTVRTSSGMFIPKSKDPIVSGIEDKIAAWTFLPKENGEDIQVL 130
           L +S  VAD +SGE   S VRTSSGMF+ K +D IV+ +E K+AAWTFLP+ENGE +Q+L
Sbjct: 69  LEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQIL 128

Query: 131 RYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSDVTKGGETVFPLAEKSPHRMASET 190
            YE+GQKY+ H+DYF DK  +  GGHR+ATVLMYLS+VTKGGETVFP  +    ++    
Sbjct: 129 HYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQL---K 188

Query: 191 DEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDSNSLHGGCPVLEGEKWSATKWIHVDS 250
           D+  S+CA++G AVKP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV S
Sbjct: 189 DDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRS 248

Query: 251 FTKNLANIGTCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRSSCRIC 301
           F K       C D +ESC+ WA  GEC KNP YMVGS    G+CR SC+ C
Sbjct: 249 FGKKKL---VCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:

BLAST of Sed0007639 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6575033.1	6.9e-156	89.67	putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022959148.1	9.0e-156	89.67	probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable pr...	[more]
XP_023547984.1	7.7e-155	89.33	probable prolyl 4-hydroxylase 4 [Cucurbita pepo subsp. pepo] >XP_023547985.1 pro...	[more]
XP_023000081.1	1.7e-154	89.67	probable prolyl 4-hydroxylase 4 [Cucurbita maxima] >XP_023000082.1 probable prol...	[more]
XP_023006272.1	2.2e-154	89.33	probable prolyl 4-hydroxylase 4 [Cucurbita maxima] >XP_023006273.1 probable prol...	[more]

BLAST of Sed0007639 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAN3	2.9e-125	72.01	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	1.6e-123	71.13	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q8L970	3.4e-97	61.28	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	1.2e-89	56.01	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q9LN20	1.3e-64	51.71	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of Sed0007639 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H545	4.4e-156	89.67	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 ...	[more]
A0A6J1KCK1	8.3e-155	89.67	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111494385 PE...	[more]
A0A6J1L4G1	1.1e-154	89.33	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE...	[more]
A0A6J1EQM4	9.1e-154	88.67	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111436810 ...	[more]
A0A1S3C816	1.3e-152	87.00	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 S...	[more]

BLAST of Sed0007639 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18900.1	2.1e-126	72.01	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	1.1e-124	71.13	P4H isoform 2	[more]
AT3G28480.1	2.4e-98	61.28	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	1.3e-91	57.66	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	8.3e-91	56.01	Oxoglutarate/iron-dependent oxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 46..246 e-value: 1.9E-62 score: 223.5
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 259..300 e-value: 0.0088 score: 25.2
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 259..300 e-value: 1.8E-4 score: 21.9
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 260..300 score: 8.17296
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 126..246 e-value: 1.5E-20 score: 73.9
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 38..247 e-value: 2.4E-75 score: 254.8
None	No IPR available	PANTHER	PTHR10869:SF175	PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEIN	coord: 7..300
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 7..300
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 122..247 score: 12.426431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0007639.1	Sed0007639.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen