Sed0007540 (gene) Chayote v1

BLAST of Sed0007540 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 592/771 (76.78%), Postives = 665/771 (86.25%), Query Frame = 0

Query: 1   MEFSY-ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSV 60
           MEFSY +SPLLLFFFLLQTSAIPTKKSYIVYLG HSFG N S YDVQ AT+S YDIL SV
Sbjct: 1   MEFSYRLSPLLLFFFLLQTSAIPTKKSYIVYLGLHSFGLNPSKYDVQRATESQYDILSSV 60

Query: 61  KGSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFL 120
            GSKLAAKESI+YSY+RYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTT+SWSFL
Sbjct: 61  TGSKLAAKESIMYSYSRYINGFAAVLDEKEAMALAKNPSVVSVFENKERKLHTTKSWSFL 120

Query: 121 GVESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGS 180
           GV+SDAG IP NSIWKAA+FG+DTIIGNLDTG WPE+KSFNDAGYGPVPSRWRG C GG+
Sbjct: 121 GVDSDAG-IPSNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWRGACDGGA 180

Query: 181 NFKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGN 240
           NF+CNRKLIGARYF++G+ M +G  N+S F TARD+ GHGSHTLSTA  NFVPGAN+FG 
Sbjct: 181 NFRCNRKLIGARYFNQGFAMANGPLNVS-FNTARDKEGHGSHTLSTAGGNFVPGANIFGY 240

Query: 241 GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEF 300
           GNGTAKGGSPKA VAAYKVCWPA  +GGC D+DILAG EAAI DGVDVLSVSLG  AQEF
Sbjct: 241 GNGTAKGGSPKARVAAYKVCWPA-PTGGCFDSDILAGFEAAIGDGVDVLSVSLGTGAQEF 300

Query: 301 AYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGN 360
           AYDAVSIGAFHA Q+GIVVVCS GNDGPSPGTV+NVSPWM TV AS++DRDF++YV LGN
Sbjct: 301 AYDAVSIGAFHAVQKGIVVVCSGGNDGPSPGTVSNVSPWMFTVAASTIDRDFASYVLLGN 360

Query: 361 KKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLR 420
           KK  +G+SLSS  L   K YPL+N ++ KAANA++ +AQFC  GSLDP KAKG IIVCLR
Sbjct: 361 KKHIRGSSLSSSGLRGRKFYPLINAIEAKAANATDSLAQFCEQGSLDPAKAKGKIIVCLR 420

Query: 421 GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 480
           G+NAR++K F V   GGVGMI+VN Q+DGS  +AD H+LPA+HV+Y DGLSI+QY+ STK
Sbjct: 421 GENARVEKSFVVLHAGGVGMIMVNDQKDGSGTLADAHILPATHVSYTDGLSISQYIKSTK 480

Query: 481 TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 540
           TPVA+IT VKTE+GIKP+PVMA F+SRGPN IT+ M+KPDITAPGV+IIASVT D TAT 
Sbjct: 481 TPVAYITHVKTEVGIKPSPVMADFSSRGPNSITEAMLKPDITAPGVNIIASVTIDATATD 540

Query: 541 MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 600
            PFDTRRVPFNVESGTSM+CPHISGV GLLKTLYPTWSPAAIKSAIMTTAKTRDNT   I
Sbjct: 541 SPFDTRRVPFNVESGTSMSCPHISGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNTQRTI 600

Query: 601 LD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFA 660
            D +K+KATPFDYGAGHVHPN+AMDPGLVYD T+DDYL FLCARGYNSVAL KF  KPF 
Sbjct: 601 SDTAKMKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNKPFI 660

Query: 661 CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 720
           C +     I D NYPSI+VP+L  G PVTVNRRVKNVG+ GTYVARVK    ++V++EPS
Sbjct: 661 CAK--SFAITDLNYPSISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEPS 720

Query: 721 KLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNIG 770
            LQFN+VGEEKAFKVVF+  GK +RQG+VFGTL+WSDGKHFVRSPIAV +G
Sbjct: 721 TLQFNSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSDGKHFVRSPIAVKLG 766

BLAST of Sed0007540 vs. NCBI nr
Match: KAG6596779.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 584/767 (76.14%), Postives = 658/767 (85.79%), Query Frame = 0

Query: 6   ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLA 65
           ISPLLLFFF+LQT+A+PTKKSYIVYLGSHSFG N SIYDVQ AT+S YDILGSVKGSK+A
Sbjct: 11  ISPLLLFFFVLQTTAVPTKKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVA 70

Query: 66  AKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESDA 125
           AK+SI+YSYNRYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTTRSW FLGV+SD 
Sbjct: 71  AKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDR 130

Query: 126 GIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFKCNR 185
           G IP NSIWKAA+FG+DTIIGNLDTGVWPE+ SFNDAGYGPVPSRWRG C+GGS F+CNR
Sbjct: 131 G-IPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNR 190

Query: 186 KLIGARYFSEGYRMIH---GHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNGNG 245
           KLIGARYF  G++         NIS F +ARD  GHGSHTLSTA  NFV G NVFGNGNG
Sbjct: 191 KLIGARYFYRGFQASERPWTKQNIS-FDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNG 250

Query: 246 TAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFAYD 305
           TAKGGS +A V AYKVCWP +++GGC D+DILAGIEAAISDGVDVLS SLG  AQEFA+D
Sbjct: 251 TAKGGSSRARVVAYKVCWP-SENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHD 310

Query: 306 AVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNKKR 365
           A+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YV LGNKKR
Sbjct: 311 AISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKR 370

Query: 366 FKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRGDN 425
           F+G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLRG+N
Sbjct: 371 FRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 430

Query: 426 ARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKTPV 485
           AR+ K FEV RVGG+GM+LVN Q DGSA++ADPH+LPASH++  DG+SI QY++STKTPV
Sbjct: 431 ARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPV 490

Query: 486 AFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATGMPF 545
           A IT   TE+GIKP+P+MASF+SRGP+ IT+ +IKPDITAPGV+IIASVT DITA+G+PF
Sbjct: 491 ASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPF 550

Query: 546 DTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPILD- 605
           D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSP AIKSAIMTTAKTRDNT + ILD 
Sbjct: 551 DKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTILDY 610

Query: 606 SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFACPR 665
           +KVKATPFDYGAGHVHPNNAMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF C +
Sbjct: 611 TKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAK 670

Query: 666 VPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPSKLQ 725
                  D NYPSI VP+L  G  VTVNRRVKNVGSAGTYVARVKMP G+ V +EPS LQ
Sbjct: 671 --NFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQ 730

Query: 726 FNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           F++VGEEK FK+VF    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 731 FHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Sed0007540 vs. NCBI nr
Match: XP_022949764.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 580/767 (75.62%), Postives = 659/767 (85.92%), Query Frame = 0

Query: 6   ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLA 65
           ISPLLLFFF+LQT+A+PT+KSYIVYLGSHSFG N SIYDVQ AT+S YDILGSVKGSK+A
Sbjct: 11  ISPLLLFFFVLQTAAVPTQKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVA 70

Query: 66  AKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESDA 125
           AK+SI+YSYNRYINGFAA+LD+ EA ALAKNP+VVS+FENKERKLHTTRSWSFLGV+SD 
Sbjct: 71  AKDSILYSYNRYINGFAAVLDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLGVDSDR 130

Query: 126 GIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFKCNR 185
           G IP NSIWKAA+FG+DTIIGNLDTGVWPE+ SFNDAGYGPVP+RWRG C GGS F+CNR
Sbjct: 131 G-IPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKFRCNR 190

Query: 186 KLIGARYFSEGYRMIH---GHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNGNG 245
           KLIGARYF  G++         NIS F +A+D  GHGSHTLSTA  NFV G NVFGNGNG
Sbjct: 191 KLIGARYFYRGFQASERPWTKQNIS-FDSAQDHEGHGSHTLSTAGGNFVHGVNVFGNGNG 250

Query: 246 TAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFAYD 305
           TAKGGSP+A V AYKVCWP +++GGC D+DILAGIEAAISDGVDVLS SLG  AQEFA+D
Sbjct: 251 TAKGGSPRARVVAYKVCWP-SENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHD 310

Query: 306 AVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNKKR 365
           A+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YVALGNKK+
Sbjct: 311 AISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQ 370

Query: 366 FKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRGDN 425
            +G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLRG+N
Sbjct: 371 LRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 430

Query: 426 ARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKTPV 485
           AR+ K FEV RVGG+GM+LVN Q DGSA++ADPH+LPASH++  DG+SI QY++STKTPV
Sbjct: 431 ARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPV 490

Query: 486 AFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATGMPF 545
           A IT   TE+GIKP+P+MASF+SRGP+ IT+ +IKPDITAPGV+IIASVT DITA+G+PF
Sbjct: 491 ASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPF 550

Query: 546 DTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPILD- 605
           D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSP AIKSAIMTTAKTRDNT + ILD 
Sbjct: 551 DKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTILDY 610

Query: 606 SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFACPR 665
           +KVKATPFDYGAGHVHPNNAMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF C +
Sbjct: 611 TKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAK 670

Query: 666 VPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPSKLQ 725
                  D NYPSI VP+L  G  VTVNRRVKNVGSAGTYVARVKMP G+ V +EPS LQ
Sbjct: 671 --NFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQ 730

Query: 726 FNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           F++VGEEK FK+VF    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 731 FHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Sed0007540 vs. NCBI nr
Match: XP_023540422.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 587/777 (75.55%), Postives = 661/777 (85.07%), Query Frame = 0

Query: 1   MEFSY-----ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDI 60
           ME SY     ISPLLLFFF+LQT A+PTKKSYIVYLGSHSFG N SIYDVQ AT+S Y I
Sbjct: 1   MEKSYYISYVISPLLLFFFMLQTVAVPTKKSYIVYLGSHSFGPNPSIYDVQLATESQYHI 60

Query: 61  LGSVKGSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRS 120
           LGSVKGSK+AAK+SI+YSYNRYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTTRS
Sbjct: 61  LGSVKGSKVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRS 120

Query: 121 WSFLGVESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVC 180
           W FLGV+SD G IP NSIWKAA+FG+DTIIGNLDTGVWPE+ SFNDAGYGPVPSRWRG C
Sbjct: 121 WGFLGVDSDRG-IPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGAC 180

Query: 181 QGGSNFKCNRKLIGARYFSEGYRMIH---GHPNISNFQTARDQRGHGSHTLSTAAANFVP 240
           +GGS F+CNRKLIGARYF  G++         NIS F +ARD  GHGSHTLSTA  NFV 
Sbjct: 181 EGGSKFRCNRKLIGARYFYRGFQASERPWTKQNIS-FDSARDHEGHGSHTLSTAGGNFVH 240

Query: 241 GANVFGNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSL 300
           G NVFGNGNGTAKGGSPKA V AYKVCWP +++GGC D+DILAGIEAAISDGVDVLS SL
Sbjct: 241 GVNVFGNGNGTAKGGSPKARVVAYKVCWP-SENGGCYDSDILAGIEAAISDGVDVLSASL 300

Query: 301 GGPAQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFS 360
           G  AQEFA DA++IGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF 
Sbjct: 301 GMAAQEFANDAIAIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFV 360

Query: 361 TYVALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKG 420
           +YV LGNKKR +G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG
Sbjct: 361 SYVVLGNKKRLRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKG 420

Query: 421 SIIVCLRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIA 480
            IIVCLRG+NAR+ K FEV RVGGVGM+LVN Q DGSA++ADPH+LPASH++Y DG+SIA
Sbjct: 421 KIIVCLRGENARVRKGFEVHRVGGVGMVLVNNQIDGSAIVADPHILPASHLSYADGVSIA 480

Query: 481 QYMNSTKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVT 540
           QY++STKTP+A IT   TE+GIKP+P+MASF+SRGP+ IT+ +IKPDITAPGV+IIASVT
Sbjct: 481 QYLSSTKTPLASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVT 540

Query: 541 TDITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTR 600
            DITA+G+ FD RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSPAAIKSAIMTTAKTR
Sbjct: 541 NDITASGLLFDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAIMTTAKTR 600

Query: 601 DNTMHPILD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSK 660
           DNT + ILD +KVKATPFDYGAGHVHPN+AMDPGLVYD TVDDYL FLC RGYNS  L K
Sbjct: 601 DNTKNTILDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKK 660

Query: 661 FSTKPFACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGV 720
           FS KPF C +       D NYPSI VP+L  G  VTVNRRVKNVGS GTYVARV+MP G+
Sbjct: 661 FSNKPFVCAK--NFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSTGTYVARVRMPKGI 720

Query: 721 AVTIEPSKLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
            V +EPSKLQF++VGEEK FK+VF    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 721 TVMVEPSKLQFHSVGEEKPFKLVFHYARKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Sed0007540 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 577/770 (74.94%), Postives = 655/770 (85.06%), Query Frame = 0

Query: 2    EFSYISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKG 61
            E++ ISPLLLFFF+L T+A+PTK SYIVYLGSHSF  N S+YDVQ AT+S YDILGSVKG
Sbjct: 770  EYNKISPLLLFFFMLHTAAVPTKNSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKG 829

Query: 62   SKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGV 121
            SK+AAK+SI+YSYNRYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTTRSW FLGV
Sbjct: 830  SKVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGV 889

Query: 122  ESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF 181
            +SD G IP NSIWKAA+FG DTIIGNLDTGVWPE+ SFNDAGYGPVPSRWRG C+GGS F
Sbjct: 890  DSDRG-IPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKF 949

Query: 182  KCNRKLIGARYFSEGYRMIHGHPNISN--FQTARDQRGHGSHTLSTAAANFVPGANVFGN 241
            +CNRKLIGARYF  G++   G     N  F +ARD  GHGSHTLSTA  NFV G NVFGN
Sbjct: 950  RCNRKLIGARYFYRGFQAAEGPLTTHNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGN 1009

Query: 242  GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEF 301
            GNGTAKGGSP+A V AYKVCWP + +GGC D+DILAGIEAAISDGVDVLS S+G  AQEF
Sbjct: 1010 GNGTAKGGSPRARVVAYKVCWP-SKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEF 1069

Query: 302  AYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGN 361
            A DA+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YV LGN
Sbjct: 1070 ANDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGN 1129

Query: 362  KKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLR 421
            KKRF+G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLR
Sbjct: 1130 KKRFRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLR 1189

Query: 422  GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 481
            G+NAR+ K FEV RVGGVGM+LVN Q DGSAL+ADPH+LPASH++Y DG+SIAQY++STK
Sbjct: 1190 GENARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTK 1249

Query: 482  TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 541
            TPVA IT   TE+GIKP+P+MASF+SRGP+ IT  +IKPDITAPGV+IIASVT DI+A+G
Sbjct: 1250 TPVASITHASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASG 1309

Query: 542  MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 601
            +P D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT + +
Sbjct: 1310 LPLDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTL 1369

Query: 602  LD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFA 661
            LD +KVKATPFDYGAGHVHPN+AMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF 
Sbjct: 1370 LDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFV 1429

Query: 662  CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 721
            C         D NYPSI VP+L  G  VTVNRRVKNVGS GTYVARV+MP G+ V +EPS
Sbjct: 1430 CAN--NFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPS 1489

Query: 722  KLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
            KLQF++VGEE+ FK++F    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 1490 KLQFHSVGEERPFKLIFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 1535

BLAST of Sed0007540 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 7.8e-239
Identity = 430/777 (55.34%), Postives = 548/777 (70.53%), Query Frame = 0

Query: 1   MEFSYISPLLLFFFLLQTS-----AIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDI 60
           M+ ++    LL   L+  S     A     SY+VY G+HS     +   +    ++HYD 
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LGSVKGSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRS 120
           LGS  GS+  A ++I YSY ++INGFAA LD + A  ++K+P VVSVF NK  KLHTTRS
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WSFLGVESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVC 180
           W FLG+E ++  +P +SIW+ A+FG+DTII NLDTGVWPE+KSF D G GP+PSRW+G+C
Sbjct: 121 WDFLGLEHNS-YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGIC 180

Query: 181 QG--GSNFKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPG 240
           Q    + F CNRKLIGARYF++GY    GH N S+F + RD  GHGSHTLSTAA +FVPG
Sbjct: 181 QNQKDATFHCNRKLIGARYFNKGYAAAVGHLN-SSFDSPRDLDGHGSHTLSTAAGDFVPG 240

Query: 241 ANVFGNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLG 300
            ++FG GNGTAKGGSP+A VAAYKVCWP      C DAD+LA  +AAI DG DV+SVSLG
Sbjct: 241 VSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLG 300

Query: 301 GPAQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFST 360
           G    F  D+V+IG+FHAA++ IVVVCSAGN GP+  TV+NV+PW ITVGAS+MDR+F++
Sbjct: 301 GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFAS 360

Query: 361 YVALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGS 420
            + LGN K +KG SLSS ALP  K YP+M  V  KA NAS   AQ C  GSLDP K KG 
Sbjct: 361 NLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGK 420

Query: 421 IIVCLRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQ 480
           I+VCLRG N R++K   V   GG+GM+L N    G+ L+ADPH+LPA+ +T  D  ++++
Sbjct: 421 ILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 480

Query: 481 YMNSTKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTT 540
           Y++ TK P+A IT  +T+LG+KPAPVMASF+S+GP+ +  +++KPDITAPGVS+IA+ T 
Sbjct: 481 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 540

Query: 541 DITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRD 600
            ++ T   FD RR+ FN  SGTSM+CPHISG+ GLLKT YP+WSPAAI+SAIMTTA   D
Sbjct: 541 AVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMD 600

Query: 601 NTMHPILD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKF 660
           +   PI + + +KATPF +GAGHV PN A++PGLVYD  + DYL FLC+ GYN+  +S F
Sbjct: 601 DIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVF 660

Query: 661 STKPFACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVA 720
           S   F C   P   +++ NYPSI VP L T + VTV+R VKNVG    Y  +V  P GV 
Sbjct: 661 SGNNFTCSS-PKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVY 720

Query: 721 VTIEPSKLQFNNVGEEKAFKVVF-KSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           V ++P+ L F  VGE+K FKV+  KS G V + GYVFG LVWSD KH VRSPI V +
Sbjct: 721 VAVKPTSLNFTKVGEQKTFKVILVKSKGNVAK-GYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sed0007540 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 1.0e-238
Identity = 431/770 (55.97%), Postives = 533/770 (69.22%), Query Frame = 0

Query: 1   MEFSYISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVK 60
           M    +S LLL   L  + A   KKSYIVYLGSH+     S   +     SH   L S  
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75

Query: 61  GSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLG 120
           GS   AKE+I YSY R+INGFAA+LDENEA  +AK+P+VVSVF NK RKLHTT SW+F+ 
Sbjct: 76  GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135

Query: 121 VESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSN 180
           +  + G++  +S+W  A +G+DTII NLDTGVWPE+KSF+D GYG VP+RW+G C    +
Sbjct: 136 LAKN-GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KD 195

Query: 181 FKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNG 240
             CNRKLIGARYF++GY    G P+ ++++T RD  GHGSHTLSTAA NFVPGANVFG G
Sbjct: 196 VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIG 255

Query: 241 NGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFA 300
           NGTA GGSPKA VAAYKVCWP  D   C DADILA IEAAI DGVDVLS S+GG A ++ 
Sbjct: 256 NGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYM 315

Query: 301 YDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNK 360
            D ++IG+FHA + G+ VVCSAGN GP  GTV+NV+PW+ITVGASSMDR+F  +V L N 
Sbjct: 316 SDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNG 375

Query: 361 KRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRG 420
           + FKG SLS   LP EK+Y L++      AN +   A  C  GSLDP K KG I+VCLRG
Sbjct: 376 QSFKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRG 435

Query: 421 DNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKT 480
           DNAR+DK  +    G  GM+L N +  G+ +I+D H+LPAS + Y DG ++  Y++STK 
Sbjct: 436 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 495

Query: 481 PVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATGM 540
           P  +I      L  KPAP MASF+SRGPN IT  ++KPDITAPGV+IIA+ T     T +
Sbjct: 496 PKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDL 555

Query: 541 PFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPIL 600
             D RR PFN ESGTSM+CPHISGVVGLLKTL+P WSPAAI+SAIMTT++TR+N   P++
Sbjct: 556 DSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV 615

Query: 601 D-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKP-FA 660
           D S  KA PF YG+GHV PN A  PGLVYD T  DYL FLCA GYN+  +  F+  P + 
Sbjct: 616 DESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYT 675

Query: 661 CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 720
           C +     ++D NYPSI VP L TG+ +TV R++KNVG   TY AR + P+GV V++EP 
Sbjct: 676 CRQ--GANLLDFNYPSITVPNL-TGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 735

Query: 721 KLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           +L FN  GE K F++  +    V   GYVFG L W+D  H+VRSPI V +
Sbjct: 736 QLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sed0007540 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 696.0 bits (1795), Expect = 4.6e-199
Identity = 392/786 (49.87%), Postives = 509/786 (64.76%), Query Frame = 0

Query: 6   ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLA 65
           +S   LF FLL  +   +KK YIVY+G+HS G + +  D++ AT SHYD+LGS+ GS+  
Sbjct: 12  LSSFFLFTFLL-AAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREK 71

Query: 66  AKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESDA 125
           AKE+IIYSYNR+INGFAALL+E EA  +AKNPNVVSVF +KE KLHTTRSW FLG+    
Sbjct: 72  AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG 131

Query: 126 GIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWR-GVCQ-----GGS 185
                NS W+  +FG++TIIGN+DTGVWPE++SF+D GYG VPS+WR G+CQ     G  
Sbjct: 132 ----QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSM 191

Query: 186 NFKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGN 245
              CNRKLIGARY+++ +   +G  +     TARD  GHG+HTLSTA  NFVPGA VF  
Sbjct: 192 KNTCNRKLIGARYYNKAFEAHNGQLD-PLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 251

Query: 246 GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLG----GP 305
           GNGTAKGGSP+A VAAYKVCW  TD   C  AD+LA I+ AI DGVDV++VS G      
Sbjct: 252 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 311

Query: 306 AQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYV 365
           A+    D +SIGAFHA  + I++V SAGNDGP+PGTV NV+PW+ T+ AS++DRDFS+ +
Sbjct: 312 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 371

Query: 366 ALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSII 425
            + N +  +GASL    LP  + + L+     K ANA+   AQ C  G+LD TK  G I+
Sbjct: 372 TI-NNQLIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIV 431

Query: 426 VCLR-GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLL------PASHVTYDDG 485
           +C R G    + +  E    G  GMIL N  ++G  L A+PH+       P    +   G
Sbjct: 432 LCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHG 491

Query: 486 L---SIAQYMNSTKT-PVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPG 545
           +   +I    +  KT     +++ +T  G KPAPVMASF+SRGPN I   ++KPD+TAPG
Sbjct: 492 VKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPG 551

Query: 546 VSIIASVTTDITATGMPFDTRR-VPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKS 605
           V+I+A+ +   +A+ +  D RR   FNV  GTSM+CPH SG+ GLLKT +P+WSPAAIKS
Sbjct: 552 VNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKS 611

Query: 606 AIMTTAKTRDNTMHPILDS--KVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCA 665
           AIMTTA T DNT  PI D+  K  A  F YG+GHV P+ A++PGLVYD ++ DYL FLCA
Sbjct: 612 AIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 671

Query: 666 RGYNSVALSKFS-TKPFACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGT 725
            GY+   +S  +  + F C       + D NYPSI +P L    PVT+ R V NVG   T
Sbjct: 672 SGYDQQLISALNFNRTFICS--GSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPST 731

Query: 726 YVARVKMPVGVAVTIEPSKLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFV 767
           Y    + P G ++ + P  L F  +GE K FKV+ +++    R+ Y FG L W+DGKH V
Sbjct: 732 YTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIV 786

BLAST of Sed0007540 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.7e-169
Identity = 340/771 (44.10%), Postives = 464/771 (60.18%), Query Frame = 0

Query: 9   LLLFFFLLQTSAIPTKKSYIVYLGS----HSFGFNSSIYDVQHATQSHYDILGSVKGSKL 68
           LL   F   +S+   + +YIV++       SF  +S+ YD           L S+  S  
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYD---------SSLRSISDS-- 73

Query: 69  AAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESD 128
                ++Y+Y   I+GF+  L + EA +L   P V+SV      +LHTTR+  FLG++  
Sbjct: 74  ---AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 133

Query: 129 AGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFK-- 188
              + P      A    D ++G LDTGVWPE+KS++D G+GP+PS W+G C+ G+NF   
Sbjct: 134 TADLFPE-----AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 193

Query: 189 -CNRKLIGARYFSEGYRMIHGHPNIS-NFQTARDQRGHGSHTLSTAAANFVPGANVFGNG 248
            CNRKLIGAR+F+ GY    G  + S   ++ RD  GHG+HT STAA + V GA++ G  
Sbjct: 194 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 253

Query: 249 NGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFA 308
           +GTA+G +P+A VA YKVCW     GGC  +DILA I+ AI+D V+VLS+SLGG   ++ 
Sbjct: 254 SGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 313

Query: 309 YDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNK 368
            D V+IGAF A +RGI+V CSAGN GPS  +++NV+PW+ TVGA ++DRDF     LGN 
Sbjct: 314 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 373

Query: 369 KRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDV-AQFCADGSLDPTKAKGSIIVCLR 428
           K F G SL  G    +KL P      + A NASN      C  G+L P K KG I++C R
Sbjct: 374 KNFTGVSLFKGEALPDKLLPF-----IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDR 433

Query: 429 GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 488
           G NAR+ K   V+  GGVGMIL N   +G  L+AD HLLPA+ V    G  I  Y+ +  
Sbjct: 434 GINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP 493

Query: 489 TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 548
            P A I+ + T +G+KP+PV+A+F+SRGPN IT  ++KPD+ APGV+I+A+ T     TG
Sbjct: 494 NPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTG 553

Query: 549 MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 608
           +  D+RRV FN+ SGTSM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA        P+
Sbjct: 554 LASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPL 613

Query: 609 LD--SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPF 668
           LD  +   +TPFD+GAGHV P  A +PGL+YD T +DYL FLCA  Y S  +   S + +
Sbjct: 614 LDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNY 673

Query: 669 ACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARV-KMPVGVAVTIE 728
            C       + D NYPS AV     G      R V +VG AGTY  +V     GV +++E
Sbjct: 674 TCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 733

Query: 729 PSKLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVN 768
           P+ L F    E+K++ V F            FG++ WSDGKH V SP+A++
Sbjct: 734 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sed0007540 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 9.8e-165
Identity = 313/723 (43.29%), Postives = 448/723 (61.96%), Query Frame = 0

Query: 62  SKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGV 121
           ++ A +  I++ Y+   +GF+A++  +EA  L  +P V++VFE++ R+LHTTRS  FLG+
Sbjct: 50  TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGL 109

Query: 122 ESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF 181
           ++  G      +W  + +G D IIG  DTG+WPE +SF+D   GP+P RWRGVC+ G+ F
Sbjct: 110 QNQKG------LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 169

Query: 182 ---KCNRKLIGARYFSEGYR--MIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANV 241
               CNRK+IGAR+F++G +  +I G      F + RD  GHG+HT STAA      A++
Sbjct: 170 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 229

Query: 242 FGNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGP- 301
            G  +G AKG +PKA +AAYKVCW      GC D+DILA  +AA+ DGVDV+S+S+GG  
Sbjct: 230 SGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGD 289

Query: 302 --AQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFST 361
                +  D ++IG++ AA +GI V  SAGN+GP+  +VTN++PW+ TVGAS++DR+F  
Sbjct: 290 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 349

Query: 362 YVALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGS 421
              LG+  R +G SL +G     +++P+     V    +    A  C + +LDP + +G 
Sbjct: 350 DAILGDGHRLRGVSLYAGVPLNGRMFPV-----VYPGKSGMSSASLCMENTLDPKQVRGK 409

Query: 422 IIVCLRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQ 481
           I++C RG + R+ K   V++ GGVGMIL NG  +G  L+ D HL+PA  V  ++G  I  
Sbjct: 410 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 469

Query: 482 YMNSTKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTT 541
           Y +S   P+A I    T +GIKPAPV+ASF+ RGPN ++ E++KPD+ APGV+I+A+ T 
Sbjct: 470 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 529

Query: 542 DITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRD 601
            +  TG+P D R+  FN+ SGTSMACPH+SG   LLK+ +P WSPA I+SA+MTT    D
Sbjct: 530 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 589

Query: 602 NTMHPILDSKV--KATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSK 661
           N+   ++D      ATP+DYG+GH++   AM+PGLVYD T DDY+ FLC+ GY    +  
Sbjct: 590 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 649

Query: 662 FSTKPFACPRVPFPVIIDHNYPSIAV--PKLNTG-TPVTVNRRVKNVGSA-GTYVARVKM 721
            +  P  CP    P   + NYPSI    P    G    TV R   NVG A   Y AR++ 
Sbjct: 650 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 709

Query: 722 PVGVAVTIEPSKLQFNNVGEEKAFKVVFKSTGK---VKRQGYVFGTLVWSD-GKHFVRSP 767
           P GV VT++P +L F +  + +++ V      +   +   G VFG++ W D GKH VRSP
Sbjct: 710 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758

BLAST of Sed0007540 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 580/767 (75.62%), Postives = 659/767 (85.92%), Query Frame = 0

Query: 6   ISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLA 65
           ISPLLLFFF+LQT+A+PT+KSYIVYLGSHSFG N SIYDVQ AT+S YDILGSVKGSK+A
Sbjct: 11  ISPLLLFFFVLQTAAVPTQKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVA 70

Query: 66  AKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESDA 125
           AK+SI+YSYNRYINGFAA+LD+ EA ALAKNP+VVS+FENKERKLHTTRSWSFLGV+SD 
Sbjct: 71  AKDSILYSYNRYINGFAAVLDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLGVDSDR 130

Query: 126 GIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFKCNR 185
           G IP NSIWKAA+FG+DTIIGNLDTGVWPE+ SFNDAGYGPVP+RWRG C GGS F+CNR
Sbjct: 131 G-IPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKFRCNR 190

Query: 186 KLIGARYFSEGYRMIH---GHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNGNG 245
           KLIGARYF  G++         NIS F +A+D  GHGSHTLSTA  NFV G NVFGNGNG
Sbjct: 191 KLIGARYFYRGFQASERPWTKQNIS-FDSAQDHEGHGSHTLSTAGGNFVHGVNVFGNGNG 250

Query: 246 TAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFAYD 305
           TAKGGSP+A V AYKVCWP +++GGC D+DILAGIEAAISDGVDVLS SLG  AQEFA+D
Sbjct: 251 TAKGGSPRARVVAYKVCWP-SENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHD 310

Query: 306 AVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNKKR 365
           A+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YVALGNKK+
Sbjct: 311 AISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQ 370

Query: 366 FKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRGDN 425
            +G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLRG+N
Sbjct: 371 LRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 430

Query: 426 ARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKTPV 485
           AR+ K FEV RVGG+GM+LVN Q DGSA++ADPH+LPASH++  DG+SI QY++STKTPV
Sbjct: 431 ARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPV 490

Query: 486 AFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATGMPF 545
           A IT   TE+GIKP+P+MASF+SRGP+ IT+ +IKPDITAPGV+IIASVT DITA+G+PF
Sbjct: 491 ASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPF 550

Query: 546 DTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPILD- 605
           D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSP AIKSAIMTTAKTRDNT + ILD 
Sbjct: 551 DKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTILDY 610

Query: 606 SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFACPR 665
           +KVKATPFDYGAGHVHPNNAMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF C +
Sbjct: 611 TKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAK 670

Query: 666 VPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPSKLQ 725
                  D NYPSI VP+L  G  VTVNRRVKNVGSAGTYVARVKMP G+ V +EPS LQ
Sbjct: 671 --NFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQ 730

Query: 726 FNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           F++VGEEK FK+VF    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 731 FHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Sed0007540 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 577/770 (74.94%), Postives = 655/770 (85.06%), Query Frame = 0

Query: 2    EFSYISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKG 61
            E++ ISPLLLFFF+L T+A+PTK SYIVYLGSHSF  N S+YDVQ AT+S YDILGSVKG
Sbjct: 770  EYNKISPLLLFFFMLHTAAVPTKNSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKG 829

Query: 62   SKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGV 121
            SK+AAK+SI+YSYNRYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTTRSW FLGV
Sbjct: 830  SKVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGV 889

Query: 122  ESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF 181
            +SD G IP NSIWKAA+FG DTIIGNLDTGVWPE+ SFNDAGYGPVPSRWRG C+GGS F
Sbjct: 890  DSDRG-IPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKF 949

Query: 182  KCNRKLIGARYFSEGYRMIHGHPNISN--FQTARDQRGHGSHTLSTAAANFVPGANVFGN 241
            +CNRKLIGARYF  G++   G     N  F +ARD  GHGSHTLSTA  NFV G NVFGN
Sbjct: 950  RCNRKLIGARYFYRGFQAAEGPLTTHNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGN 1009

Query: 242  GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEF 301
            GNGTAKGGSP+A V AYKVCWP + +GGC D+DILAGIEAAISDGVDVLS S+G  AQEF
Sbjct: 1010 GNGTAKGGSPRARVVAYKVCWP-SKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEF 1069

Query: 302  AYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGN 361
            A DA+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YV LGN
Sbjct: 1070 ANDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGN 1129

Query: 362  KKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLR 421
            KKRF+G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLR
Sbjct: 1130 KKRFRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLR 1189

Query: 422  GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 481
            G+NAR+ K FEV RVGGVGM+LVN Q DGSAL+ADPH+LPASH++Y DG+SIAQY++STK
Sbjct: 1190 GENARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTK 1249

Query: 482  TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 541
            TPVA IT   TE+GIKP+P+MASF+SRGP+ IT  +IKPDITAPGV+IIASVT DI+A+G
Sbjct: 1250 TPVASITHASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASG 1309

Query: 542  MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 601
            +P D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT + +
Sbjct: 1310 LPLDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTL 1369

Query: 602  LD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFA 661
            LD +KVKATPFDYGAGHVHPN+AMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF 
Sbjct: 1370 LDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFV 1429

Query: 662  CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 721
            C         D NYPSI VP+L  G  VTVNRRVKNVGS GTYVARV+MP G+ V +EPS
Sbjct: 1430 CAN--NFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPS 1489

Query: 722  KLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
            KLQF++VGEE+ FK++F    K++RQGYVFG LVWSDGKHFVRS IAVN+
Sbjct: 1490 KLQFHSVGEERPFKLIFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 1535

BLAST of Sed0007540 vs. ExPASy TrEMBL
Match: A0A6J1KYS5 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498344 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 577/771 (74.84%), Postives = 655/771 (84.95%), Query Frame = 0

Query: 2   EFSYISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKG 61
           E++ ISPLLLFFF+L T+A+PTK SYIVYLGSHSF  N S+YDVQ AT+S YDILGSVKG
Sbjct: 3   EYNKISPLLLFFFMLHTAAVPTKNSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKG 62

Query: 62  SKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGV 121
           SK+AAK+SI+YSYNRYINGFAA+LDE EA ALAKNP+VVSVFENKERKLHTTRSW FLGV
Sbjct: 63  SKVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGV 122

Query: 122 ESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF 181
           +SD G IP NSIWKAA+FG DTIIGNLDTGVWPE+ SFNDAGYGPVPSRWRG C+GGS F
Sbjct: 123 DSDRG-IPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKF 182

Query: 182 KCNRKLIGARYFSEGYRMIHGHPNISN--FQTARDQRGHGSHTLSTAAANFVPGANVFGN 241
           +CNRKLIGARYF  G++   G     N  F +ARD  GHGSHTLSTA  NFV G NVFGN
Sbjct: 183 RCNRKLIGARYFYRGFQAAEGPLTTHNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGN 242

Query: 242 GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEF 301
           GNGTAKGGSP+A V AYKVCWP + +GGC D+DILAGIEAAISDGVDVLS S+G  AQEF
Sbjct: 243 GNGTAKGGSPRARVVAYKVCWP-SKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEF 302

Query: 302 AYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGN 361
           A DA+SIGAFHA Q GIVVVCSAGNDGPSPG+V+NVSPWM+TVGAS++DRDF +YV LGN
Sbjct: 303 ANDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGN 362

Query: 362 KKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLR 421
           KKRF+G+SLSS  LPA K YPL+  VQVKAANA++  AQ C DG+LDPTKAKG IIVCLR
Sbjct: 363 KKRFRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLR 422

Query: 422 GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 481
           G+NAR+ K FEV RVGGVGM+LVN Q DGSAL+ADPH+LPASH++Y DG+SIAQY++STK
Sbjct: 423 GENARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTK 482

Query: 482 TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 541
           TPVA IT   TE+GIKP+P+MASF+SRGP+ IT  +IKPDITAPGV+IIASVT DI+A+G
Sbjct: 483 TPVASITHASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASG 542

Query: 542 MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 601
           +P D RRVPFN+ESGTSM+CPHISGV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT + +
Sbjct: 543 LPLDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTL 602

Query: 602 LD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPFA 661
           LD +KVKATPFDYGAGHVHPN+AMDPGLVYD TVDDYL FLC RGYNS  L KFS KPF 
Sbjct: 603 LDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFV 662

Query: 662 CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 721
           C         D NYPSI VP+L  G  VTVNRRVKNVGS GTYVARV+MP G+ V +EPS
Sbjct: 663 CAN--NFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPS 722

Query: 722 KLQFNNVGEEKAFKVVFKSTGKVKRQ-GYVFGTLVWSDGKHFVRSPIAVNI 769
           KLQF++VGEE+ FK++F    K++RQ GYVFG LVWSDGKHFVRS IAVN+
Sbjct: 723 KLQFHSVGEERPFKLIFHYAHKLRRQGGYVFGALVWSDGKHFVRSSIAVNL 769

BLAST of Sed0007540 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 576/772 (74.61%), Postives = 648/772 (83.94%), Query Frame = 0

Query: 1   MEFSY-ISPLLLFFFLLQTSAIPTKKSYIVYLGSHS--FGFNSSIYDVQHATQSHYDILG 60
           MEFSY ISPLLLFFFLL  S++ TKKSYIVYLGS S  FG N SIYDVQ AT+S YDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHASSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  SVKGSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWS 120
           +VKGSK+AAKESI+Y+YNR INGFAA+LD+NE  ALAKNP+VVSVFEN+ERKLHTTRSW 
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQG 180
           FLGV+S  G IP NSIWKA+KFG+D IIGNLDTGVWPE++SF+DAGYGPVPSRW G C+G
Sbjct: 121 FLGVDSGRG-IPQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEG 180

Query: 181 GSNFKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVF 240
           GS F+CNRKLIGARYF  GY MI+G  NIS+   ARD  GHG+HTLSTA  NFV GANVF
Sbjct: 181 GSKFRCNRKLIGARYFYRGYEMINGPLNISSL-NARDHEGHGTHTLSTAGGNFVQGANVF 240

Query: 241 GNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQ 300
           GNGNGTAKGG+PKA VAAYKVCWP    G CSDAD+LAGIEAAISDGVDVLS+SLG  AQ
Sbjct: 241 GNGNGTAKGGAPKARVAAYKVCWP---QGKCSDADLLAGIEAAISDGVDVLSISLGATAQ 300

Query: 301 EFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVAL 360
           +FA D +S+GAFHA Q+GI+VVCSAGNDGP PGTVTNVSPWM TVGASS+DR F +YV L
Sbjct: 301 DFANDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYL 360

Query: 361 GNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVC 420
           GNKK+ KG+SLSSG LP  KLYPLMN V  KA+NAS+ +AQ C +GSLDP KA+G IIVC
Sbjct: 361 GNKKQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVC 420

Query: 421 LRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNS 480
           LRGDN RMDK FEV RVGGVGMILVN +  GS +  DPH+LP SHV+Y DGLSIAQY+ S
Sbjct: 421 LRGDNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKS 480

Query: 481 TKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITA 540
           TK PVA IT V+TE+GIKP+PVMA+F+SRGPN IT+ +IKPDITAPGV+IIAS T    A
Sbjct: 481 TKRPVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAA 540

Query: 541 TGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMH 600
           T +PFD RRVPFNV+SGTSM+CPHISGV GLLK L+PTWSPAAIKSAIMTTAKTRDNT +
Sbjct: 541 TDLPFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKN 600

Query: 601 PILD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKP 660
            +LD +KVKATPFDYGAG VHPNNAMDPGLVYD T+DDYL FLC +GYNS+ L KFS KP
Sbjct: 601 TMLDFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKP 660

Query: 661 FACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIE 720
           F C +     I D NYPSI+VPKL  G PVTVNRRVKNVGSAGTYVARV+MP G+ V +E
Sbjct: 661 FVCSK--NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVE 720

Query: 721 PSKLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           PS LQF+ VGEEK FK+VF    +V+R GYVFG LVWSDGKHFVRSPIAVN+
Sbjct: 721 PSTLQFHGVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNL 765

BLAST of Sed0007540 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 554/745 (74.36%), Postives = 629/745 (84.43%), Query Frame = 0

Query: 26  SYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLAAKESIIYSYNRYINGFAALL 85
           SYIVYLG+ S G N + YD++ AT+S YD+LGSV GSKLAAK++I YSYN+YINGFAA L
Sbjct: 6   SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 65

Query: 86  DENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESDAGIIPPNSIWKAAKFGQDTII 145
           DE +AK LAKNP VVSVFENKERKLHTTRSW FLGVESD G IP NSIW A +FG+DTII
Sbjct: 66  DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEG-IPSNSIWNAGRFGEDTII 125

Query: 146 GNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFKCNRKLIGARYFSEGYRMIHGHPN 205
           GNLDTGVWPE+KSFNDAGYGPVPSRWRG C+GG+NF+CNRKLIGARYF++G+ M  G  N
Sbjct: 126 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 185

Query: 206 ISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNGNGTAKGGSPKACVAAYKVCWPATDS 265
           IS F TARD++GHGSHTLSTA  NFVPGANVFG GNGTAKGGSPKA VAAYKVCWPAT  
Sbjct: 186 IS-FNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSG 245

Query: 266 GGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFAYDAVSIGAFHAAQRGIVVVCSAGND 325
           GGC DADILAG EAAISDGVDVLSVSLG   +EFAYD++SIGAFHA Q+GIVVVCSAGND
Sbjct: 246 GGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGND 305

Query: 326 GPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNKKRFKGASLSSGALPAEKLYPLMNGV 385
           GP PGTV+N+SPWM TV ASS+DRDF++Y +LGNKK +KG+S+SS AL   K YPL+N V
Sbjct: 306 GPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAV 365

Query: 386 QVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRGDNARMDKDFEVRRVGGVGMILVNGQ 445
             KAANAS  +AQ C  GSLDPTKAKG IIVCLRG+NAR++K F V + GGVGMILVNG+
Sbjct: 366 DAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGK 425

Query: 446 EDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKTPVAFITQVKTELGIKPAPVMASFTS 505
             GS   AD H+LPA+H++Y DGL++AQY+NSTKTPVA IT V+T+LGIKP+PVMA F+S
Sbjct: 426 NGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSS 485

Query: 506 RGPNPITDEMIKPDITAPGVSIIASVTTDITATGMPFDTRRVPFNVESGTSMACPHISGV 565
           RGPNPIT+ M+KPDIT PG+SI+ASVTTD+TAT  PFDTRRVPFNVESGTSM+CPHISGV
Sbjct: 486 RGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGV 545

Query: 566 VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPILDS-KVKATPFDYGAGHVHPNNAMDP 625
           VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTM  I D+ K KATPFDYGAGHVHPN+AMDP
Sbjct: 546 VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDP 605

Query: 626 GLVYDATVDDYLLFLCARGYNSVALSKFSTKPFACPRVPFPVIIDHNYPSIAVPKLNTGT 685
           GLVYD T+DDYL FLCARGYNS+    F  KPF C +     + D NYPSI++PKL  G 
Sbjct: 606 GLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAK--SFTLTDLNYPSISIPKLQFGA 665

Query: 686 PVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPSKLQFNNVGEEKAFKVVFKSTGKVKRQ 745
           PVTVNRRVKNVG+ GTYVARV     + VT+EPS LQFN+VGEEKAFKVVF+  G  + +
Sbjct: 666 PVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDK 725

Query: 746 GYVFGTLVWSDGKHFVRSPIAVNIG 770
           GYVFGTL+WSDGKH VRSPI VN+G
Sbjct: 726 GYVFGTLIWSDGKHNVRSPIVVNLG 746

BLAST of Sed0007540 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 828.2 bits (2138), Expect = 5.6e-240
Identity = 430/777 (55.34%), Postives = 548/777 (70.53%), Query Frame = 0

Query: 1   MEFSYISPLLLFFFLLQTS-----AIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDI 60
           M+ ++    LL   L+  S     A     SY+VY G+HS     +   +    ++HYD 
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LGSVKGSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRS 120
           LGS  GS+  A ++I YSY ++INGFAA LD + A  ++K+P VVSVF NK  KLHTTRS
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WSFLGVESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVC 180
           W FLG+E ++  +P +SIW+ A+FG+DTII NLDTGVWPE+KSF D G GP+PSRW+G+C
Sbjct: 121 WDFLGLEHNS-YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGIC 180

Query: 181 QG--GSNFKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPG 240
           Q    + F CNRKLIGARYF++GY    GH N S+F + RD  GHGSHTLSTAA +FVPG
Sbjct: 181 QNQKDATFHCNRKLIGARYFNKGYAAAVGHLN-SSFDSPRDLDGHGSHTLSTAAGDFVPG 240

Query: 241 ANVFGNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLG 300
            ++FG GNGTAKGGSP+A VAAYKVCWP      C DAD+LA  +AAI DG DV+SVSLG
Sbjct: 241 VSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLG 300

Query: 301 GPAQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFST 360
           G    F  D+V+IG+FHAA++ IVVVCSAGN GP+  TV+NV+PW ITVGAS+MDR+F++
Sbjct: 301 GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFAS 360

Query: 361 YVALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGS 420
            + LGN K +KG SLSS ALP  K YP+M  V  KA NAS   AQ C  GSLDP K KG 
Sbjct: 361 NLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGK 420

Query: 421 IIVCLRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQ 480
           I+VCLRG N R++K   V   GG+GM+L N    G+ L+ADPH+LPA+ +T  D  ++++
Sbjct: 421 ILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 480

Query: 481 YMNSTKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTT 540
           Y++ TK P+A IT  +T+LG+KPAPVMASF+S+GP+ +  +++KPDITAPGVS+IA+ T 
Sbjct: 481 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 540

Query: 541 DITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRD 600
            ++ T   FD RR+ FN  SGTSM+CPHISG+ GLLKT YP+WSPAAI+SAIMTTA   D
Sbjct: 541 AVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMD 600

Query: 601 NTMHPILD-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKF 660
           +   PI + + +KATPF +GAGHV PN A++PGLVYD  + DYL FLC+ GYN+  +S F
Sbjct: 601 DIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVF 660

Query: 661 STKPFACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVA 720
           S   F C   P   +++ NYPSI VP L T + VTV+R VKNVG    Y  +V  P GV 
Sbjct: 661 SGNNFTCSS-PKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVY 720

Query: 721 VTIEPSKLQFNNVGEEKAFKVVF-KSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           V ++P+ L F  VGE+K FKV+  KS G V + GYVFG LVWSD KH VRSPI V +
Sbjct: 721 VAVKPTSLNFTKVGEQKTFKVILVKSKGNVAK-GYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sed0007540 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 827.8 bits (2137), Expect = 7.2e-240
Identity = 431/770 (55.97%), Postives = 533/770 (69.22%), Query Frame = 0

Query: 1   MEFSYISPLLLFFFLLQTSAIPTKKSYIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVK 60
           M    +S LLL   L  + A   KKSYIVYLGSH+     S   +     SH   L S  
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75

Query: 61  GSKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLG 120
           GS   AKE+I YSY R+INGFAA+LDENEA  +AK+P+VVSVF NK RKLHTT SW+F+ 
Sbjct: 76  GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135

Query: 121 VESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSN 180
           +  + G++  +S+W  A +G+DTII NLDTGVWPE+KSF+D GYG VP+RW+G C    +
Sbjct: 136 LAKN-GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KD 195

Query: 181 FKCNRKLIGARYFSEGYRMIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANVFGNG 240
             CNRKLIGARYF++GY    G P+ ++++T RD  GHGSHTLSTAA NFVPGANVFG G
Sbjct: 196 VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIG 255

Query: 241 NGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFA 300
           NGTA GGSPKA VAAYKVCWP  D   C DADILA IEAAI DGVDVLS S+GG A ++ 
Sbjct: 256 NGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYM 315

Query: 301 YDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNK 360
            D ++IG+FHA + G+ VVCSAGN GP  GTV+NV+PW+ITVGASSMDR+F  +V L N 
Sbjct: 316 SDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNG 375

Query: 361 KRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCLRG 420
           + FKG SLS   LP EK+Y L++      AN +   A  C  GSLDP K KG I+VCLRG
Sbjct: 376 QSFKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRG 435

Query: 421 DNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTKT 480
           DNAR+DK  +    G  GM+L N +  G+ +I+D H+LPAS + Y DG ++  Y++STK 
Sbjct: 436 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 495

Query: 481 PVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATGM 540
           P  +I      L  KPAP MASF+SRGPN IT  ++KPDITAPGV+IIA+ T     T +
Sbjct: 496 PKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDL 555

Query: 541 PFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPIL 600
             D RR PFN ESGTSM+CPHISGVVGLLKTL+P WSPAAI+SAIMTT++TR+N   P++
Sbjct: 556 DSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV 615

Query: 601 D-SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKP-FA 660
           D S  KA PF YG+GHV PN A  PGLVYD T  DYL FLCA GYN+  +  F+  P + 
Sbjct: 616 DESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYT 675

Query: 661 CPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARVKMPVGVAVTIEPS 720
           C +     ++D NYPSI VP L TG+ +TV R++KNVG   TY AR + P+GV V++EP 
Sbjct: 676 CRQ--GANLLDFNYPSITVPNL-TGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 735

Query: 721 KLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVNI 769
           +L FN  GE K F++  +    V   GYVFG L W+D  H+VRSPI V +
Sbjct: 736 QLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sed0007540 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-170
Identity = 340/771 (44.10%), Postives = 464/771 (60.18%), Query Frame = 0

Query: 9   LLLFFFLLQTSAIPTKKSYIVYLGS----HSFGFNSSIYDVQHATQSHYDILGSVKGSKL 68
           LL   F   +S+   + +YIV++       SF  +S+ YD           L S+  S  
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYD---------SSLRSISDS-- 73

Query: 69  AAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVESD 128
                ++Y+Y   I+GF+  L + EA +L   P V+SV      +LHTTR+  FLG++  
Sbjct: 74  ---AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 133

Query: 129 AGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNFK-- 188
              + P      A    D ++G LDTGVWPE+KS++D G+GP+PS W+G C+ G+NF   
Sbjct: 134 TADLFPE-----AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 193

Query: 189 -CNRKLIGARYFSEGYRMIHGHPNIS-NFQTARDQRGHGSHTLSTAAANFVPGANVFGNG 248
            CNRKLIGAR+F+ GY    G  + S   ++ RD  GHG+HT STAA + V GA++ G  
Sbjct: 194 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 253

Query: 249 NGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEFA 308
           +GTA+G +P+A VA YKVCW     GGC  +DILA I+ AI+D V+VLS+SLGG   ++ 
Sbjct: 254 SGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 313

Query: 309 YDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGNK 368
            D V+IGAF A +RGI+V CSAGN GPS  +++NV+PW+ TVGA ++DRDF     LGN 
Sbjct: 314 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 373

Query: 369 KRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDV-AQFCADGSLDPTKAKGSIIVCLR 428
           K F G SL  G    +KL P      + A NASN      C  G+L P K KG I++C R
Sbjct: 374 KNFTGVSLFKGEALPDKLLPF-----IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDR 433

Query: 429 GDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMNSTK 488
           G NAR+ K   V+  GGVGMIL N   +G  L+AD HLLPA+ V    G  I  Y+ +  
Sbjct: 434 GINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP 493

Query: 489 TPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTTDITATG 548
            P A I+ + T +G+KP+PV+A+F+SRGPN IT  ++KPD+ APGV+I+A+ T     TG
Sbjct: 494 NPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTG 553

Query: 549 MPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMHPI 608
           +  D+RRV FN+ SGTSM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA        P+
Sbjct: 554 LASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPL 613

Query: 609 LD--SKVKATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSKFSTKPF 668
           LD  +   +TPFD+GAGHV P  A +PGL+YD T +DYL FLCA  Y S  +   S + +
Sbjct: 614 LDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNY 673

Query: 669 ACPRVPFPVIIDHNYPSIAVPKLNTGTPVTVNRRVKNVGSAGTYVARV-KMPVGVAVTIE 728
            C       + D NYPS AV     G      R V +VG AGTY  +V     GV +++E
Sbjct: 674 TCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 733

Query: 729 PSKLQFNNVGEEKAFKVVFKSTGKVKRQGYVFGTLVWSDGKHFVRSPIAVN 768
           P+ L F    E+K++ V F            FG++ WSDGKH V SP+A++
Sbjct: 734 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sed0007540 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 582.0 bits (1499), Expect = 6.9e-166
Identity = 313/723 (43.29%), Postives = 448/723 (61.96%), Query Frame = 0

Query: 62  SKLAAKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGV 121
           ++ A +  I++ Y+   +GF+A++  +EA  L  +P V++VFE++ R+LHTTRS  FLG+
Sbjct: 50  TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGL 109

Query: 122 ESDAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF 181
           ++  G      +W  + +G D IIG  DTG+WPE +SF+D   GP+P RWRGVC+ G+ F
Sbjct: 110 QNQKG------LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 169

Query: 182 ---KCNRKLIGARYFSEGYR--MIHGHPNISNFQTARDQRGHGSHTLSTAAANFVPGANV 241
               CNRK+IGAR+F++G +  +I G      F + RD  GHG+HT STAA      A++
Sbjct: 170 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 229

Query: 242 FGNGNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGP- 301
            G  +G AKG +PKA +AAYKVCW      GC D+DILA  +AA+ DGVDV+S+S+GG  
Sbjct: 230 SGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGD 289

Query: 302 --AQEFAYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFST 361
                +  D ++IG++ AA +GI V  SAGN+GP+  +VTN++PW+ TVGAS++DR+F  
Sbjct: 290 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 349

Query: 362 YVALGNKKRFKGASLSSGALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGS 421
              LG+  R +G SL +G     +++P+     V    +    A  C + +LDP + +G 
Sbjct: 350 DAILGDGHRLRGVSLYAGVPLNGRMFPV-----VYPGKSGMSSASLCMENTLDPKQVRGK 409

Query: 422 IIVCLRGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQ 481
           I++C RG + R+ K   V++ GGVGMIL NG  +G  L+ D HL+PA  V  ++G  I  
Sbjct: 410 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 469

Query: 482 YMNSTKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVTT 541
           Y +S   P+A I    T +GIKPAPV+ASF+ RGPN ++ E++KPD+ APGV+I+A+ T 
Sbjct: 470 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 529

Query: 542 DITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRD 601
            +  TG+P D R+  FN+ SGTSMACPH+SG   LLK+ +P WSPA I+SA+MTT    D
Sbjct: 530 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 589

Query: 602 NTMHPILDSKV--KATPFDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALSK 661
           N+   ++D      ATP+DYG+GH++   AM+PGLVYD T DDY+ FLC+ GY    +  
Sbjct: 590 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 649

Query: 662 FSTKPFACPRVPFPVIIDHNYPSIAV--PKLNTG-TPVTVNRRVKNVGSA-GTYVARVKM 721
            +  P  CP    P   + NYPSI    P    G    TV R   NVG A   Y AR++ 
Sbjct: 650 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 709

Query: 722 PVGVAVTIEPSKLQFNNVGEEKAFKVVFKSTGK---VKRQGYVFGTLVWSD-GKHFVRSP 767
           P GV VT++P +L F +  + +++ V      +   +   G VFG++ W D GKH VRSP
Sbjct: 710 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758

BLAST of Sed0007540 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 573.9 bits (1478), Expect = 1.9e-163
Identity = 340/786 (43.26%), Postives = 462/786 (58.78%), Query Frame = 0

Query: 12  FFFLLQTSAIPTKKS------YIVYLGSHSFGFNSSIYDVQHATQSHYDILGSVKGSKLA 71
           FFFLL  S+  +  S      YIV++   +     SI+       +H+    S   S  +
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEA---KPSIF------PTHFHWYTSSLASLTS 66

Query: 72  AKESIIYSYNRYINGFAALLDENEAKALAKNPNVVSVFENKERKLHTTRSWSFLGVES-- 131
           +  SII++Y+   +GF+A L   +A  L  +P+V+SV   + R LHTTRS  FLG+ S  
Sbjct: 67  SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 126

Query: 132 DAGIIPPNSIWKAAKFGQDTIIGNLDTGVWPEAKSFNDAGYGPVPSRWRGVCQGGSNF-- 191
            AG++      + + FG D +IG +DTGVWPE  SF+D G GPVP +W+G C    +F  
Sbjct: 127 KAGLL------EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPE 186

Query: 192 -KCNRKLIGARYFSEGYRMIHGHPN-ISNFQTARDQRGHGSHTLSTAAANFVPGANVFGN 251
             CNRKL+GAR+F  GY   +G  N  + F++ RD  GHG+HT S +A  +V  A+  G 
Sbjct: 187 SACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGY 246

Query: 252 GNGTAKGGSPKACVAAYKVCWPATDSGGCSDADILAGIEAAISDGVDVLSVSLGGPAQEF 311
            +G A G +PKA +AAYKVCW    + GC D+DILA  + A++DGVDV+S+S+GG    +
Sbjct: 247 AHGVAAGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPY 306

Query: 312 AYDAVSIGAFHAAQRGIVVVCSAGNDGPSPGTVTNVSPWMITVGASSMDRDFSTYVALGN 371
             DA++IGAF A  RGI V  SAGN GP   TVTNV+PWM TVGA ++DRDF   V LGN
Sbjct: 307 YLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGN 366

Query: 372 KKRFKGASLSSG-ALPAEKLYPLMNGVQVKAANASNDVAQFCADGSLDPTKAKGSIIVCL 431
            K   G S+  G  L   ++YPL+ G  +   +  +  +  C +GSLDP   KG I++C 
Sbjct: 367 GKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS--SSLCLEGSLDPNLVKGKIVLCD 426

Query: 432 RGDNARMDKDFEVRRVGGVGMILVNGQEDGSALIADPHLLPASHVTYDDGLSIAQYMN-- 491
           RG N+R  K   VR+ GG+GMI+ NG  DG  L+AD H+LPA+ V    G  I +Y++  
Sbjct: 427 RGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISES 486

Query: 492 ----STKTPVAFITQVKTELGIKPAPVMASFTSRGPNPITDEMIKPDITAPGVSIIASVT 551
               S+K P A I    T LGI+PAPV+ASF++RGPNP T E++KPD+ APG++I+A+  
Sbjct: 487 SKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWP 546

Query: 552 TDITATGMPFDTRRVPFNVESGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTR 611
             I  +G+  D RR  FN+ SGTSMACPH+SG+  LLK  +P WSPAAI+SA++TTA T 
Sbjct: 547 DRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTV 606

Query: 612 DNTMHPILDSKVKATP--FDYGAGHVHPNNAMDPGLVYDATVDDYLLFLCARGYNSVALS 671
           DN+  P++D     T    DYG+GHVHP  AMDPGLVYD T  DY+ FLC   Y    + 
Sbjct: 607 DNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIV 666

Query: 672 KFSTKPFACPRVPFPVIIDH-NYPSIAVPKLNTGTPVTVN---RRVKNVG-SAGTYVARV 731
             + +   C        + + NYPS +V     G         R V NVG S   Y  ++
Sbjct: 667 TITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKI 726

Query: 732 KMPVGVAVTIEPSKLQFNNVGEEKAFKVVFKSTGKVKRQGYV---FGTLVWSDGKHFVRS 769
           + P G  VT+EP KL F  VG++ +F V  K+T      G      G +VWSDGK  V S
Sbjct: 727 RPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTS 771