Sed0007258 (gene) Chayote v1

Overview
NameSed0007258
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG07: 6660772 .. 6664648 (+)
RNA-Seq ExpressionSed0007258
SyntenySed0007258
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTATCTCAACCCTTTGACTTCAAGTGCATCCTATTTGTACATTTCTTCTTCACTTCAACTCTTCAATTTTCATCCCATCCCTATATTTTATACCCAAAATTTGCATATATAATTAGGATTTGTACATTTCTACACTCTTCTCCATATACTCCACAAATCATGAATACTAATCTACTCACTAACATTTTCCTTTCCTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCACTCCTCGCTTTCTCTACGACCATGCCGCCCTCGACTTCACCTTCCTTGACGACTCCATCAGTTGTCATCACCATCATCATAAAAGCTCGACAGATGACGATGAAGATGAAAACCAATCAACTATCAATTCCATTTGTGACATGTTCCCGACAAGTCTTCTCCCACCAGAAACCAACAACACTTCGGTCTTCTGTGTCGATCGAAATGGATGTTGTAACTTCACGACAGTTCAGGCTGCTGTCGATGCAGTGCCAAACATGAGTCTGAAAAGAAGCTTGATATGGATTAATAGTGGAATCTACTAGTAATTAATTTTAAATTAATTTATTTTTTCTTCTTTTTTTATTTCTTGGTTTAAAAGGGAGTTTTTTTTTTTTTTTTTGGAATTGTGTAGTGAGAAGGTAATGGTTGCAAGATCAAAACCTAACGTGACATTTCAAGGACAAGGCTACTTAACCACAGCTATTGTTTGGAATGACACAGCCAACTCTTCCCATGGGACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCCACCAATTTTATTGCCAAAAATATAAGCTTCATGGTAAATTATAATACTGGTATATTTCTTGTACAACAGTTATTCTATTTATTATAAAAAATCATCAAAACGTAATTGTTTTACCTAAAACAATCATTATAAAGAAATTATTACTTTTTATTGATTTAAAAAATTTTAAAATAATTTTTAGAAATAATTTATAATTATATTGGAGATTTTTCATTTATAATTTTTTTTTTAAAAAACAACTTTTTAAAAGAAATTTATGCATAACAAACAATTGAAGTGGGTGTAGTAAAAAGACGAAAAAAGTCATGCATTCGATCACTTTAAACCGAGCAACGGATGGTCTTTAATCTGTGCACATATATAATCTTCAATCAATGTCATAAATAGTCGTCAGTCTACAGCTCTCAAATAAACACCTATTTTTCAAGAAATCTCATGTGATCGTCGTAATGAACTGAATTACAACCAAACAAAACCACTAATCCTTTCGGTTGGAGGAGGAGAACCACATAATAACTAGTTGGTTACTTTTTTTTCTTTCTTTTTTTTAAGTACAAATTGAGGAACTTTTTTTTAGTACGTATTGACGGAGGGAGATTACAAACTTTACATAGGGGGTTTCATTGCTGTCAATTAAATGAGAGCCAAATGCCGATTATTGGGATCGATTATGTGACTATGGGATTGAATATTGATTGTCTAGATCAAATGTGTAACCATAGTGTTGAATTGATTTACAACCAAGTCACTCCCTTATTTGTCTAAAATGTTACTTAATTAAAATATATAAAAAAATAATAATAAATAAATTATGGTTGTATGTTATGACCTCGTTGTCATAAATATTCACATTAAAATTATAAAACAAAAATTAAAAATTTTGTTCAATAACTACTGTTGTTAAACTTCTTTTTAACTTTTTTTTTTGTCAACAAAGTAATGTACAACCATTCCCCGCCCCAATCGTTATCGTTGTAATCTAAAAAAAAGATAAGAAAAGCTATTAATTTTACGGATTAATCAGCATAATAAATTATGTTCACCGAATTTCACTTTTAAATTTTATCAATAAGCCAATATTTGGGGGATCTGGCAGAATGTGGCCCCAATTCCAGGGCCGGGCGACATCGGAGCACAGGCGGTGGCGATAAGAATCGGCGGCGACCAGGCAGCGTTTTGGAGCTGCGGATTCTTCGGCGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAAATCCTTCTACGAGGTAAATCTGTTCTGAAACCCTAATTTTCTCTCCAATTTCAATTCGACAATGAAATTGCTAACCATTTCGAAGATTTTTAATCGGTTCCGAAACCCTAATTGTCTGTCTGATTTCGATTTGACGATGAAATTACTAATGATTTCGAAGATTTCAAATCGGTTCCGAAACCCTAATTTTCTGTCCGATTTCAATTTGCTAACGATTTTGAAGATTTGAAATCGCAGAGTTGCCAGTTGATCTCGATGGCGAATCCGGTGCCGCAAGGATCGAAGTTTGTGAATGGAGCCGTGACGGCACACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTTCGTTTGTTAATTGCAGCATCGGCGGCACCGGCAGGGTGTGGCTCGGCCGTGCGTGGCGGCCGTTCTCCAGCGTCGTCTTCGCCAACACCATCATGACGGATATCATTGCGCCTGAGGGATGGAATGACTTTAATGACCCTGCCAGAGACCAGTAAGTTTTTCTTAATTTTATACTTAGAGGGGCTATTTGTTTGACATATTTAGGTAGTATTTAATTTTAGAATTTATTAATTTCTCATCGAGAATCTGCATCCAAACAAGTTTTTACTAATTACTTTTCATTTTTACTCTCATTTTTAAACATTTTTAATCATTAATTTATAACTACTCAATCATTTCTAACTACTCATTTTTAGTCATTCATTTCAAATCAATCAAAATAATTCATTTTTAATCATTTCTTTTTTAACTACTCATTTTAAATCTACATCCAAACAAGATTTTGATTCTAACTTCTCTACACATAAACTATATCCAAACGAGTTTTTTACTACAACTCTCAATTTTTAACTACTCAAATTAACTCCTCAATATAACTACTTAAATTAAATCCTACTTAAATATGTCAAACAAACAACCCGAGAAAGTTTACAAGAAGTTGGATATTCTGATTATATTTCTCACTTACTAAAGGTCTAAAGTAAAAAATTATAATTTAGCATAAGATGAAATTAAATCATACCCGTTTTATAATTCACTCAAAGGAATAGAAAAAACAAATTTAACTTATAATAGGTAAACAAAAATTGAACATTTTTTCAAAGTTTAAGGTTAAAATTGCAATATTTATGAGTGTTGACCTAAATTGATGAAATTGAAAATTTAACTAAAAAAATTAAAACTGACTTTAATGACATGATACATACTTAGTAAGCAAGAGAACCTTCAAATATTGTTATTATACTACAAAATATTGAATAACTAAAATGAAAATAAACCACATATATTGGATAAAATACTAGGCAACTCATTTAATGTGGTCATCTAATGAAAATCCTGGTATAAATGTGTATCTAACATAATGAATTAATATTTCTCATAATAACAATTTTTTCTTTTAAATTTTTATATTCATAATCTTTTAATCATTGAATATATATATATCGATAATATTGAGCTATTATTTTTGTGGTTCGTAATAGCAAAATGAGATTCATAAAATAAATAAATATTATCATTTAACAACATTACATTGAATGATAGGACAATTTTCTACGGAGAATACAACTGCAGTGGAACAGGAGCCAACATGAGTGCAAGGGCTCCCTTTGTTCAGAGGCTGAATGATACACAAGTTTCTCCTTATCTCAACTTGTCCTTCATTGATGCAGATCAATGGCTTCAACCCTATTCATCTATTTAACATTCTCTATTCATTCTTTTCTAATTTTTATATCTATACATATATTCTTTTCATTCACATTCTCATTCTCATTTAATTAATAATTATATATTAAGTTATTTCTTATG

mRNA sequence

ATGCTATCTCAACCCTTTGACTTCAAGTGCATCCTATTTGTACATTTCTTCTTCACTTCAACTCTTCAATTTTCATCCCATCCCTATATTTTATACCCAAAATTTGCATATATAATTAGGATTTGTACATTTCTACACTCTTCTCCATATACTCCACAAATCATGAATACTAATCTACTCACTAACATTTTCCTTTCCTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCACTCCTCGCTTTCTCTACGACCATGCCGCCCTCGACTTCACCTTCCTTGACGACTCCATCAGTTGTCATCACCATCATCATAAAAGCTCGACAGATGACGATGAAGATGAAAACCAATCAACTATCAATTCCATTTGTGACATGTTCCCGACAAGTCTTCTCCCACCAGAAACCAACAACACTTCGGTCTTCTGTGTCGATCGAAATGGATGTTGTAACTTCACGACAGTTCAGGCTGCTGTCGATGCAGTGCCAAACATGAGTCTGAAAAGAAGCTTGATATGGATTAATAGTGGAATCTACTATGAGAAGGTAATGGTTGCAAGATCAAAACCTAACGTGACATTTCAAGGACAAGGCTACTTAACCACAGCTATTGTTTGGAATGACACAGCCAACTCTTCCCATGGGACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCCACCAATTTTATTGCCAAAAATATAAGCTTCATGAATGTGGCCCCAATTCCAGGGCCGGGCGACATCGGAGCACAGGCGGTGGCGATAAGAATCGGCGGCGACCAGGCAGCGTTTTGGAGCTGCGGATTCTTCGGCGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAAATCCTTCTACGAGAGTTGCCAGTTGATCTCGATGGCGAATCCGGTGCCGCAAGGATCGAAGTTTGTGAATGGAGCCGTGACGGCACACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTTCGTTTGTTAATTGCAGCATCGGCGGCACCGGCAGGGTGTGGCTCGGCCGTGCGTGGCGGCCGTTCTCCAGCGTCGTCTTCGCCAACACCATCATGACGGATATCATTGCGCCTGAGGGATGGAATGACTTTAATGACCCTGCCAGAGACCAGACAATTTTCTACGGAGAATACAACTGCAGTGGAACAGGAGCCAACATGAGTGCAAGGGCTCCCTTTGTTCAGAGGCTGAATGATACACAAGTTTCTCCTTATCTCAACTTGTCCTTCATTGATGCAGATCAATGGCTTCAACCCTATTCATCTATTTAACATTCTCTATTCATTCTTTTCTAATTTTTATATCTATACATATATTCTTTTCATTCACATTCTCATTCTCATTTAATTAATAATTATATATTAAGTTATTTCTTATG

Coding sequence (CDS)

ATGCTATCTCAACCCTTTGACTTCAAGTGCATCCTATTTGTACATTTCTTCTTCACTTCAACTCTTCAATTTTCATCCCATCCCTATATTTTATACCCAAAATTTGCATATATAATTAGGATTTGTACATTTCTACACTCTTCTCCATATACTCCACAAATCATGAATACTAATCTACTCACTAACATTTTCCTTTCCTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCACTCCTCGCTTTCTCTACGACCATGCCGCCCTCGACTTCACCTTCCTTGACGACTCCATCAGTTGTCATCACCATCATCATAAAAGCTCGACAGATGACGATGAAGATGAAAACCAATCAACTATCAATTCCATTTGTGACATGTTCCCGACAAGTCTTCTCCCACCAGAAACCAACAACACTTCGGTCTTCTGTGTCGATCGAAATGGATGTTGTAACTTCACGACAGTTCAGGCTGCTGTCGATGCAGTGCCAAACATGAGTCTGAAAAGAAGCTTGATATGGATTAATAGTGGAATCTACTATGAGAAGGTAATGGTTGCAAGATCAAAACCTAACGTGACATTTCAAGGACAAGGCTACTTAACCACAGCTATTGTTTGGAATGACACAGCCAACTCTTCCCATGGGACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCCACCAATTTTATTGCCAAAAATATAAGCTTCATGAATGTGGCCCCAATTCCAGGGCCGGGCGACATCGGAGCACAGGCGGTGGCGATAAGAATCGGCGGCGACCAGGCAGCGTTTTGGAGCTGCGGATTCTTCGGCGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAAATCCTTCTACGAGAGTTGCCAGTTGATCTCGATGGCGAATCCGGTGCCGCAAGGATCGAAGTTTGTGAATGGAGCCGTGACGGCACACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTTCGTTTGTTAATTGCAGCATCGGCGGCACCGGCAGGGTGTGGCTCGGCCGTGCGTGGCGGCCGTTCTCCAGCGTCGTCTTCGCCAACACCATCATGACGGATATCATTGCGCCTGAGGGATGGAATGACTTTAATGACCCTGCCAGAGACCAGACAATTTTCTACGGAGAATACAACTGCAGTGGAACAGGAGCCAACATGAGTGCAAGGGCTCCCTTTGTTCAGAGGCTGAATGATACACAAGTTTCTCCTTATCTCAACTTGTCCTTCATTGATGCAGATCAATGGCTTCAACCCTATTCATCTATTTAA

Protein sequence

MLSQPFDFKCILFVHFFFTSTLQFSSHPYILYPKFAYIIRICTFLHSSPYTPQIMNTNLLTNIFLSSLIPLLAFLLINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDEDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPYLNLSFIDADQWLQPYSSI
Homology
BLAST of Sed0007258 vs. NCBI nr
Match: XP_022136420.1 (probable pectinesterase 8 [Momordica charantia])

HSP 1 Score: 682.9 bits (1761), Expect = 1.8e-192
Identity = 333/394 (84.52%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 56  NTNLLTNIFLSSLIPLLAFLLIN-PTPRFLYDHAALDFTFLDDSISC-----HHHHHKSS 115
           N N L  I LSSLIPLLAFLLIN P PRFL D   LDF FL D ++C     HHHHH+  
Sbjct: 3   NLNNLMTIALSSLIPLLAFLLINHPAPRFLSD---LDFAFLRDHVTCRRHHHHHHHHRRH 62

Query: 116 TDDDEDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLK 175
               ++E+Q+  +SICDMFP SLLP ETN TSVFCVDRNGCCNFTTVQAAVDAVPN+SLK
Sbjct: 63  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLSLK 122

Query: 176 RSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTN 235
           R+LIWINSGIYYEKVMV RSK NVTF+GQGY TTAIVWNDTANSSHGTFYS SVQVFS+N
Sbjct: 123 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 182

Query: 236 FIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDC 295
           FIAKNISFMNVAPIPGPGD+GAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFKDC
Sbjct: 183 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 242

Query: 296 YIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCS 355
           YIQGSIDFIFGNG+SFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCS
Sbjct: 243 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 302

Query: 356 IGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGAN 415
           + GTGRVWLGRAWRPFS VVF NT+MTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GAN
Sbjct: 303 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 362

Query: 416 MSARAPFVQRLNDTQVSPYLNLSFIDADQWLQPY 444
             ARAPFVQRLNDTQVSP+LNLSFIDADQWL PY
Sbjct: 363 TGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

BLAST of Sed0007258 vs. NCBI nr
Match: XP_008451885.1 (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 678.3 bits (1749), Expect = 4.4e-191
Identity = 323/388 (83.25%), Postives = 354/388 (91.24%), Query Frame = 0

Query: 58  NLLTNIFLSSLIPLLAFL-LINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDEDE 117
           N L N+ LSSL+PLLAFL +INPTP +L      +     D + C H HH    +D +++
Sbjct: 10  NFLINLTLSSLLPLLAFLFMINPTPSYL-----SNLVVFGDPVDCPHPHHHHHHEDPDED 69

Query: 118 NQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWIN 177
           +QS I+SICD FPT+L+PPET NTSVFCVD+NGCCNFTTVQAAVDAVPN+S+KR++IWIN
Sbjct: 70  DQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSIKRNIIWIN 129

Query: 178 SGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNIS 237
           +GIYYEKVMV +SKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAKNIS
Sbjct: 130 AGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNIS 189

Query: 238 FMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID 297
           FMNVAPIPGPGD+GAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID
Sbjct: 190 FMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID 249

Query: 298 FIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRV 357
           FIFGNG+SFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGFSFVNCSIGGTGRV
Sbjct: 250 FIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRV 309

Query: 358 WLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPF 417
           WLGRAWRPFS VVFANT+MTDIIAPEGWNDFNDP RDQTIFYGEYNCSG GANMS+RAP+
Sbjct: 310 WLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPY 369

Query: 418 VQRLNDTQVSPYLNLSFIDADQWLQPYS 445
           VQRLNDTQVS +LNLSFIDADQWLQPYS
Sbjct: 370 VQRLNDTQVSSFLNLSFIDADQWLQPYS 392

BLAST of Sed0007258 vs. NCBI nr
Match: KAG7015100.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675.2 bits (1741), Expect = 3.7e-190
Identity = 332/388 (85.57%), Postives = 355/388 (91.49%), Query Frame = 0

Query: 58  NLLTNIFLSSLIPL-LAFLL-INPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDED 117
           N+L NI LSSL+PL LA LL + PTP FL        + LDDS+SCHHHHHKSS D   +
Sbjct: 5   NVLINIALSSLVPLFLALLLVVIPTPPFL--------SVLDDSLSCHHHHHKSSAD---E 64

Query: 118 ENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWI 177
           E++++I SICD FPT+LLPPET NTSV CVD+NGCCNFTTVQAAVDAV NMS KRSLIWI
Sbjct: 65  EDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWI 124

Query: 178 NSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNI 237
           N+GIY+EKVMV RSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAKNI
Sbjct: 125 NTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNI 184

Query: 238 SFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSI 297
           SFMNVAPIPGPG IGAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSI
Sbjct: 185 SFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSI 244

Query: 298 DFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGR 357
           DFIFGNG+S+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGR
Sbjct: 245 DFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGR 304

Query: 358 VWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAP 417
           VWLGRAWRPFS VVFANT+MTDIIA EGWNDFNDPARDQTIFYGEYNCSG GANMSARAP
Sbjct: 305 VWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAP 364

Query: 418 FVQRLNDTQVSPYLNLSFIDADQWLQPY 444
           +VQRLNDTQ SP+LNLSFIDADQWLQPY
Sbjct: 365 YVQRLNDTQASPFLNLSFIDADQWLQPY 381

BLAST of Sed0007258 vs. NCBI nr
Match: XP_022931352.1 (probable pectinesterase 8 [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 4.9e-190
Identity = 332/390 (85.13%), Postives = 355/390 (91.03%), Query Frame = 0

Query: 56  NTNLLTNIFLSSLIPL-LAFLL-INPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDD 115
           + NLL NI LSSL+PL LA LL + PTP F        F+  DDS+SCHHHHHK S D  
Sbjct: 3   HNNLLINIALSSLVPLFLALLLVVIPTPPF--------FSVPDDSLSCHHHHHKPSAD-- 62

Query: 116 EDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLI 175
            +E++++I SICD FPT+LLPPET NTSV CVD+NGCCNFTTVQAAVDAV NMS KRSLI
Sbjct: 63  -EEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLI 122

Query: 176 WINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAK 235
           WIN+GIY+EKVMV RSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAK
Sbjct: 123 WINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAK 182

Query: 236 NISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQG 295
           NISFMNVAPIPGPG IGAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQG
Sbjct: 183 NISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQG 242

Query: 296 SIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGT 355
           SIDFIFGNG+S+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGT
Sbjct: 243 SIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGT 302

Query: 356 GRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSAR 415
           GRVWLGRAWRPFS VVFANT+MTDIIA EGWNDFNDPARDQTIFYGEYNCSG GANMSAR
Sbjct: 303 GRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSAR 362

Query: 416 APFVQRLNDTQVSPYLNLSFIDADQWLQPY 444
           AP+VQRLNDTQVSP+LNLSFIDADQWLQPY
Sbjct: 363 APYVQRLNDTQVSPFLNLSFIDADQWLQPY 381

BLAST of Sed0007258 vs. NCBI nr
Match: KAG6577098.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.9 bits (1740), Expect = 4.9e-190
Identity = 331/388 (85.31%), Postives = 355/388 (91.49%), Query Frame = 0

Query: 58  NLLTNIFLSSLIPL-LAFLL-INPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDED 117
           N+L NI LSSL+PL LA LL + PTP FL        + LDDS+SCHHHHHKSS D   +
Sbjct: 5   NVLINIALSSLVPLFLALLLVVIPTPPFL--------SVLDDSLSCHHHHHKSSAD---E 64

Query: 118 ENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWI 177
           E++++I SICD FPT+LLPPET NTS+ CVD+NGCCNFTTVQAAVDAV NMS KRSLIWI
Sbjct: 65  EDRASIGSICDTFPTALLPPETVNTSILCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWI 124

Query: 178 NSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNI 237
           N+GIY+EKVMV RSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAKNI
Sbjct: 125 NTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNI 184

Query: 238 SFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSI 297
           SFMNVAPIPGPG IGAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSI
Sbjct: 185 SFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSI 244

Query: 298 DFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGR 357
           DFIFGNG+S+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGR
Sbjct: 245 DFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGR 304

Query: 358 VWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAP 417
           VWLGRAWRPFS VVFANT+MTDIIA EGWNDFNDPARDQTIFYGEYNCSG GANMSARAP
Sbjct: 305 VWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAP 364

Query: 418 FVQRLNDTQVSPYLNLSFIDADQWLQPY 444
           +VQRLNDTQ SP+LNLSFIDADQWLQPY
Sbjct: 365 YVQRLNDTQASPFLNLSFIDADQWLQPY 381

BLAST of Sed0007258 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 515.4 bits (1326), Expect = 6.5e-145
Identity = 246/377 (65.25%), Postives = 297/377 (78.78%), Query Frame = 0

Query: 67  SLIPLLAFLLINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDEDENQSTIN--SI 126
           ++I +LA   +  T    +   A+ ++F       HHHHH         +   T    SI
Sbjct: 13  AIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHHHHHHHRHKPSDTKRKVSI 72

Query: 127 CDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKV 186
           CD FP ++ P +T+ TS  CVD+NGCCNFTTVQ+AVDAV N S +R++IWINSG+YYEKV
Sbjct: 73  CDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKV 132

Query: 187 MVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNISFMNVAPIP 246
           ++ ++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQVF + F+AKNISFMNVAPIP
Sbjct: 133 VIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIP 192

Query: 247 GPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKS 306
            PGD+GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN KS
Sbjct: 193 KPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKS 252

Query: 307 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRP 366
            Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGFSFVNC+IGGTG VWLGRAWRP
Sbjct: 253 LYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRP 312

Query: 367 FSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQ 426
           +S VVF +T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+MS RAP+VQ+LN+TQ
Sbjct: 313 YSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQ 372

Query: 427 VSPYLNLSFIDADQWLQ 442
           V+  +N SFID DQWLQ
Sbjct: 373 VALLINTSFIDGDQWLQ 389

BLAST of Sed0007258 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 4.0e-94
Identity = 172/340 (50.59%), Postives = 232/340 (68.24%), Query Frame = 0

Query: 102 HHHHHKSSTDDDEDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVD 161
           HHHHH          +   I   C+ + TS L  +   + V  VD +GC NF+ VQ+A+D
Sbjct: 52  HHHHHHHHHHHHHYHHHEPI-KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAID 111

Query: 162 AVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSG 221
            VP++S  ++LI +NSG Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S 
Sbjct: 112 VVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSF 171

Query: 222 SVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDR 281
           S  VF+ NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+
Sbjct: 172 SFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDK 231

Query: 282 GRHYFKDCYIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENS 341
           GRH+FK+C+IQGSIDFIFGNG+S Y+ C + S+A     G   V G++TA GR S DE S
Sbjct: 232 GRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQS 291

Query: 342 GFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEY 401
           GFSFVNC I G+G + LGRAW  +++VVF+NT M+ II PEGWN++ D  +++T+ +GE+
Sbjct: 292 GFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEH 351

Query: 402 NCSGTGANMSARAPFVQRLNDTQVSPYLNLSFIDADQWLQ 442
            C G GA+   R  F ++L D++ S ++++SFID D+WL+
Sbjct: 352 KCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of Sed0007258 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.2e-85
Identity = 151/306 (49.35%), Postives = 204/306 (66.67%), Query Frame = 0

Query: 135 PETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVT 194
           P    T V  V  NGC  F  VQ A+DA    S  ++LI I+ GIY E+ +V  +K N+ 
Sbjct: 34  PTKGFTMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLV 93

Query: 195 FQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAV 254
            QG GY  T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG + AQAV
Sbjct: 94  VQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAV 153

Query: 255 AIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYESCQLISM 314
           A+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNG+S YE C L S+
Sbjct: 154 ALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSI 213

Query: 315 ANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTI 374
           A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++ V+F+ T 
Sbjct: 214 A------KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTY 273

Query: 375 MTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPYLNLSFI 434
           M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  + + L+D + +P+ N+SFI
Sbjct: 274 MSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFI 333

Query: 435 DADQWL 441
           D ++WL
Sbjct: 334 DGEEWL 333

BLAST of Sed0007258 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.6e-79
Identity = 139/296 (46.96%), Postives = 197/296 (66.55%), Query Frame = 0

Query: 152 NFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTA 211
           +FT +Q A+D++P ++  R +I +++G+Y EKV +   K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 212 NSSH------GTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAF 271
            +        GT+ S S  V S  F+AKNI+F N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 272 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFV 331
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 332 NGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWN 391
            GAVTA GR+S  E++GFSFV C + GTG ++LGRAW PFS VVFA T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 392 DFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPYLNLSFIDADQWLQ 442
           ++ DP+R+ T+FYG+Y C+G GAN   R  + + L D +  P+L+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Sed0007258 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 9.9e-69
Identity = 141/313 (45.05%), Postives = 188/313 (60.06%), Query Frame = 0

Query: 137 TNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQ 196
           TN   V  VD+NG  +  TVQ AVD VP+ + +R  I+I  GIY EKV+V +SKP ++F 
Sbjct: 76  TNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFI 135

Query: 197 G-QGYL-TTAIVWNDTAN------SSHGTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGD 256
           G + Y   T I W+D A+         GT+ + SV + S  F A  I+F N   +   G+
Sbjct: 136 GNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGE 195

Query: 257 IGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYES 316
            G QAVA+RI GD+A F+     G+QDTL DD G HYF  CYIQG++DFIFGN KS Y+ 
Sbjct: 196 QGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQD 255

Query: 317 CQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSV 376
           C + S A           GA+ AH R S  E++GFSFVNC I GTG+++LGRAW  +S  
Sbjct: 256 CDIHSTAKRY--------GAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRT 315

Query: 377 VFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPY 436
           V++N  + DII P GW+D+  P R + + +GEYNC G GA    R P+ + L   +V P+
Sbjct: 316 VYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPF 375

Query: 437 LNLSFIDADQWLQ 442
           L   FI  DQWL+
Sbjct: 376 LGREFIYGDQWLR 379

BLAST of Sed0007258 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 8.7e-193
Identity = 333/394 (84.52%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 56  NTNLLTNIFLSSLIPLLAFLLIN-PTPRFLYDHAALDFTFLDDSISC-----HHHHHKSS 115
           N N L  I LSSLIPLLAFLLIN P PRFL D   LDF FL D ++C     HHHHH+  
Sbjct: 3   NLNNLMTIALSSLIPLLAFLLINHPAPRFLSD---LDFAFLRDHVTCRRHHHHHHHHRRH 62

Query: 116 TDDDEDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLK 175
               ++E+Q+  +SICDMFP SLLP ETN TSVFCVDRNGCCNFTTVQAAVDAVPN+SLK
Sbjct: 63  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLSLK 122

Query: 176 RSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTN 235
           R+LIWINSGIYYEKVMV RSK NVTF+GQGY TTAIVWNDTANSSHGTFYS SVQVFS+N
Sbjct: 123 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 182

Query: 236 FIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDC 295
           FIAKNISFMNVAPIPGPGD+GAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFKDC
Sbjct: 183 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 242

Query: 296 YIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCS 355
           YIQGSIDFIFGNG+SFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCS
Sbjct: 243 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 302

Query: 356 IGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGAN 415
           + GTGRVWLGRAWRPFS VVF NT+MTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GAN
Sbjct: 303 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 362

Query: 416 MSARAPFVQRLNDTQVSPYLNLSFIDADQWLQPY 444
             ARAPFVQRLNDTQVSP+LNLSFIDADQWL PY
Sbjct: 363 TGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

BLAST of Sed0007258 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.1e-191
Identity = 323/388 (83.25%), Postives = 354/388 (91.24%), Query Frame = 0

Query: 58  NLLTNIFLSSLIPLLAFL-LINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDEDE 117
           N L N+ LSSL+PLLAFL +INPTP +L      +     D + C H HH    +D +++
Sbjct: 10  NFLINLTLSSLLPLLAFLFMINPTPSYL-----SNLVVFGDPVDCPHPHHHHHHEDPDED 69

Query: 118 NQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWIN 177
           +QS I+SICD FPT+L+PPET NTSVFCVD+NGCCNFTTVQAAVDAVPN+S+KR++IWIN
Sbjct: 70  DQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSIKRNIIWIN 129

Query: 178 SGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNIS 237
           +GIYYEKVMV +SKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAKNIS
Sbjct: 130 AGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNIS 189

Query: 238 FMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID 297
           FMNVAPIPGPGD+GAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID
Sbjct: 190 FMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSID 249

Query: 298 FIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRV 357
           FIFGNG+SFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGFSFVNCSIGGTGRV
Sbjct: 250 FIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRV 309

Query: 358 WLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPF 417
           WLGRAWRPFS VVFANT+MTDIIAPEGWNDFNDP RDQTIFYGEYNCSG GANMS+RAP+
Sbjct: 310 WLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPY 369

Query: 418 VQRLNDTQVSPYLNLSFIDADQWLQPYS 445
           VQRLNDTQVS +LNLSFIDADQWLQPYS
Sbjct: 370 VQRLNDTQVSSFLNLSFIDADQWLQPYS 392

BLAST of Sed0007258 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.4e-190
Identity = 332/390 (85.13%), Postives = 355/390 (91.03%), Query Frame = 0

Query: 56  NTNLLTNIFLSSLIPL-LAFLL-INPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDD 115
           + NLL NI LSSL+PL LA LL + PTP F        F+  DDS+SCHHHHHK S D  
Sbjct: 3   HNNLLINIALSSLVPLFLALLLVVIPTPPF--------FSVPDDSLSCHHHHHKPSAD-- 62

Query: 116 EDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLI 175
            +E++++I SICD FPT+LLPPET NTSV CVD+NGCCNFTTVQAAVDAV NMS KRSLI
Sbjct: 63  -EEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLI 122

Query: 176 WINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAK 235
           WIN+GIY+EKVMV RSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAK
Sbjct: 123 WINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAK 182

Query: 236 NISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQG 295
           NISFMNVAPIPGPG IGAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQG
Sbjct: 183 NISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQG 242

Query: 296 SIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGT 355
           SIDFIFGNG+S+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGT
Sbjct: 243 SIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGT 302

Query: 356 GRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSAR 415
           GRVWLGRAWRPFS VVFANT+MTDIIA EGWNDFNDPARDQTIFYGEYNCSG GANMSAR
Sbjct: 303 GRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSAR 362

Query: 416 APFVQRLNDTQVSPYLNLSFIDADQWLQPY 444
           AP+VQRLNDTQVSP+LNLSFIDADQWLQPY
Sbjct: 363 APYVQRLNDTQVSPFLNLSFIDADQWLQPY 381

BLAST of Sed0007258 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.1e-187
Identity = 329/388 (84.79%), Postives = 352/388 (90.72%), Query Frame = 0

Query: 58  NLLTNIFLSSLIPL-LAFLL-INPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDED 117
           N+L NI LSSL+PL LA LL + PTP FL        + LDDS++CHHHHH   + D+ED
Sbjct: 5   NVLINIALSSLVPLFLALLLVVIPTPPFL--------SVLDDSLNCHHHHHHKPSADEED 64

Query: 118 ENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWI 177
             Q++I SICD FPT+LLP ET +TSV CVD NGCCNFTTVQAAVDAV NMS KRSLIWI
Sbjct: 65  --QTSIGSICDTFPTALLPLETVDTSVLCVDLNGCCNFTTVQAAVDAVLNMSSKRSLIWI 124

Query: 178 NSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNI 237
           N+GIY+EKVMV RSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS+NFIAKNI
Sbjct: 125 NTGIYFEKVMVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNI 184

Query: 238 SFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSI 297
           SFMNVAPIPGPG IGAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSI
Sbjct: 185 SFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSI 244

Query: 298 DFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGR 357
           DFIFGNG+S+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGR
Sbjct: 245 DFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGR 304

Query: 358 VWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAP 417
           VWLGRAWRPFS VVFANT+MTDIIA EGWNDFNDPARDQTIFYGEYNCSGTGANMSARAP
Sbjct: 305 VWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAP 364

Query: 418 FVQRLNDTQVSPYLNLSFIDADQWLQPY 444
           +VQRLNDTQ SP+LNLSFIDADQWLQPY
Sbjct: 365 YVQRLNDTQASPFLNLSFIDADQWLQPY 382

BLAST of Sed0007258 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.5e-187
Identity = 322/394 (81.73%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 54  IMNTN-LLTNIFLSSLIPLLAFL-LINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTD 113
           IMN N  L N+ LSSL+PLLAFL +INPTP FL      +     D + C HHHH     
Sbjct: 4   IMNYNHFLINLTLSSLLPLLAFLFMINPTPSFL-----SNLVVFGDPVDCPHHHHPHHHH 63

Query: 114 DDEDE-NQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKR 173
           ++ DE +QS I+SICD FPT+L+P ET NTS+FCVD+N CCNFTT+QAAVDAVPN+S+KR
Sbjct: 64  ENPDEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKR 123

Query: 174 SLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNF 233
           ++IWIN+GIYYEKVMV ++K NVTFQGQGY TTAIVWNDTANSSHGTFYS SVQVFS+NF
Sbjct: 124 NIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 183

Query: 234 IAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCY 293
           IAKN+SFMNVAPIP PGD+GAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCY
Sbjct: 184 IAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCY 243

Query: 294 IQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSI 353
           IQGSIDFIFGNG+SFYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSI
Sbjct: 244 IQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSI 303

Query: 354 GGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANM 413
           GGTGRVWLGRAWRPFS VVFANT+MTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GANM
Sbjct: 304 GGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANM 363

Query: 414 SARAPFVQRLNDTQVSPYLNLSFIDADQWLQPYS 445
           S+RAP+VQRLNDTQVS +LNLSFIDADQWLQPYS
Sbjct: 364 SSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPYS 392

BLAST of Sed0007258 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.6e-146
Identity = 246/377 (65.25%), Postives = 297/377 (78.78%), Query Frame = 0

Query: 67  SLIPLLAFLLINPTPRFLYDHAALDFTFLDDSISCHHHHHKSSTDDDEDENQSTIN--SI 126
           ++I +LA   +  T    +   A+ ++F       HHHHH         +   T    SI
Sbjct: 13  AIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHHHHHHHRHKPSDTKRKVSI 72

Query: 127 CDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKV 186
           CD FP ++ P +T+ TS  CVD+NGCCNFTTVQ+AVDAV N S +R++IWINSG+YYEKV
Sbjct: 73  CDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKV 132

Query: 187 MVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNISFMNVAPIP 246
           ++ ++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQVF + F+AKNISFMNVAPIP
Sbjct: 133 VIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIP 192

Query: 247 GPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKS 306
            PGD+GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN KS
Sbjct: 193 KPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKS 252

Query: 307 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRP 366
            Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGFSFVNC+IGGTG VWLGRAWRP
Sbjct: 253 LYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRP 312

Query: 367 FSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQ 426
           +S VVF +T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+MS RAP+VQ+LN+TQ
Sbjct: 313 YSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQ 372

Query: 427 VSPYLNLSFIDADQWLQ 442
           V+  +N SFID DQWLQ
Sbjct: 373 VALLINTSFIDGDQWLQ 389

BLAST of Sed0007258 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.8e-95
Identity = 172/340 (50.59%), Postives = 232/340 (68.24%), Query Frame = 0

Query: 102 HHHHHKSSTDDDEDENQSTINSICDMFPTSLLPPETNNTSVFCVDRNGCCNFTTVQAAVD 161
           HHHHH          +   I   C+ + TS L  +   + V  VD +GC NF+ VQ+A+D
Sbjct: 52  HHHHHHHHHHHHHYHHHEPI-KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAID 111

Query: 162 AVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTANSSHGTFYSG 221
            VP++S  ++LI +NSG Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S 
Sbjct: 112 VVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSF 171

Query: 222 SVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDR 281
           S  VF+ NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+
Sbjct: 172 SFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDK 231

Query: 282 GRHYFKDCYIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENS 341
           GRH+FK+C+IQGSIDFIFGNG+S Y+ C + S+A     G   V G++TA GR S DE S
Sbjct: 232 GRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQS 291

Query: 342 GFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWNDFNDPARDQTIFYGEY 401
           GFSFVNC I G+G + LGRAW  +++VVF+NT M+ II PEGWN++ D  +++T+ +GE+
Sbjct: 292 GFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEH 351

Query: 402 NCSGTGANMSARAPFVQRLNDTQVSPYLNLSFIDADQWLQ 442
            C G GA+   R  F ++L D++ S ++++SFID D+WL+
Sbjct: 352 KCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of Sed0007258 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 8.3e-87
Identity = 151/306 (49.35%), Postives = 204/306 (66.67%), Query Frame = 0

Query: 135 PETNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVT 194
           P    T V  V  NGC  F  VQ A+DA    S  ++LI I+ GIY E+ +V  +K N+ 
Sbjct: 34  PTKGFTMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLV 93

Query: 195 FQGQGYLTTAIVWNDTANSSHGTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAV 254
            QG GY  T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG + AQAV
Sbjct: 94  VQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAV 153

Query: 255 AIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYESCQLISM 314
           A+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNG+S YE C L S+
Sbjct: 154 ALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSI 213

Query: 315 ANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTI 374
           A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++ V+F+ T 
Sbjct: 214 A------KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTY 273

Query: 375 MTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPYLNLSFI 434
           M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  + + L+D + +P+ N+SFI
Sbjct: 274 MSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFI 333

Query: 435 DADQWL 441
           D ++WL
Sbjct: 334 DGEEWL 333

BLAST of Sed0007258 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.6e-80
Identity = 139/296 (46.96%), Postives = 197/296 (66.55%), Query Frame = 0

Query: 152 NFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQGQGYLTTAIVWNDTA 211
           +FT +Q A+D++P ++  R +I +++G+Y EKV +   K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 212 NSSH------GTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGDIGAQAVAIRIGGDQAAF 271
            +        GT+ S S  V S  F+AKNI+F N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 272 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYESCQLISMANPVPQGSKFV 331
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 332 NGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSVVFANTIMTDIIAPEGWN 391
            GAVTA GR+S  E++GFSFV C + GTG ++LGRAW PFS VVFA T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 392 DFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPYLNLSFIDADQWLQ 442
           ++ DP+R+ T+FYG+Y C+G GAN   R  + + L D +  P+L+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Sed0007258 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 7.0e-70
Identity = 141/313 (45.05%), Postives = 188/313 (60.06%), Query Frame = 0

Query: 137 TNNTSVFCVDRNGCCNFTTVQAAVDAVPNMSLKRSLIWINSGIYYEKVMVARSKPNVTFQ 196
           TN   V  VD+NG  +  TVQ AVD VP+ + +R  I+I  GIY EKV+V +SKP ++F 
Sbjct: 76  TNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFI 135

Query: 197 G-QGYL-TTAIVWNDTAN------SSHGTFYSGSVQVFSTNFIAKNISFMNVAPIPGPGD 256
           G + Y   T I W+D A+         GT+ + SV + S  F A  I+F N   +   G+
Sbjct: 136 GNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGE 195

Query: 257 IGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGKSFYES 316
            G QAVA+RI GD+A F+     G+QDTL DD G HYF  CYIQG++DFIFGN KS Y+ 
Sbjct: 196 QGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQD 255

Query: 317 CQLISMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRPFSSV 376
           C + S A           GA+ AH R S  E++GFSFVNC I GTG+++LGRAW  +S  
Sbjct: 256 CDIHSTAKRY--------GAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRT 315

Query: 377 VFANTIMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPFVQRLNDTQVSPY 436
           V++N  + DII P GW+D+  P R + + +GEYNC G GA    R P+ + L   +V P+
Sbjct: 316 VYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPF 375

Query: 437 LNLSFIDADQWLQ 442
           L   FI  DQWL+
Sbjct: 376 LGREFIYGDQWLR 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136420.11.8e-19284.52probable pectinesterase 8 [Momordica charantia][more]
XP_008451885.14.4e-19183.25PREDICTED: probable pectinesterase 8 [Cucumis melo][more]
KAG7015100.13.7e-19085.57putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931352.14.9e-19085.13probable pectinesterase 8 [Cucurbita moschata][more]
KAG6577098.14.9e-19085.31putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
O230386.5e-14565.25Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2[more]
Q9ZQA34.0e-9450.59Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9ZQA41.2e-8549.35Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1[more]
Q8VYZ33.6e-7946.96Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9FM799.9e-6945.05Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C3U98.7e-19384.52Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1[more]
A0A1S3BRY72.1e-19183.25Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1[more]
A0A6J1ETE22.4e-19085.13Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1[more]
A0A6J1J3X11.1e-18784.79Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1[more]
A0A0A0KVA95.5e-18781.73Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G05310.14.6e-14665.25Pectin lyase-like superfamily protein [more]
AT2G36710.12.8e-9550.59Pectin lyase-like superfamily protein [more]
AT2G36700.18.3e-8749.35Pectin lyase-like superfamily protein [more]
AT5G19730.12.6e-8046.96Pectin lyase-like superfamily protein [more]
AT5G55590.17.0e-7045.05Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 145..436
e-value: 4.6E-68
score: 229.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 141..445
e-value: 1.3E-98
score: 332.2
NoneNo IPR availablePANTHERPTHR31321:SF33PECTINESTERASE 8-RELATEDcoord: 96..444
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 96..444
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 291..300
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 142..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0007258.1Sed0007258.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity