Sed0007082 (gene) Chayote v1

Overview


Name	Sed0007082
Type	gene
Organism	Sechium edule (Chayote v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	LG07: 32290367 .. 32292463 (+)
RNA-Seq Expression	Sed0007082
Synteny	Sed0007082

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCTCTCTTGTCTTCCTTGTGGGACAATAAAGAAGGGAAGAACAATGCCTGAAGCACCAAACACAAGGTCTCTGGTGGAGAAAAACAAGGCCGCTCTGGAATTCATAGAGGACATCACGAGCAACGCCGGCCACGTCCAGCGCCAAGTGCTCTCCCAAATCCTCTCCGGCAACGCCTCCTCCGAGTACTTAACCCTCCACGGGCGCCCCAATTCCCCTGACACCTTCAAGTCCACCCTCCCTTTAGTCTCCTACGACCAAATCCAACCCTTCATCTCCCGCATCGCCAACGGCGACACCTCCCCCATCCTCTCCTCCTCCCCCATCTCCGAGTTCCTCACCAGCTCCGGCACCTCCGCCGGCGAGCGCAAGCTCATGCCCACCATCCACCAAGATCTCGACCGCCGCTCCCTCTTGTACAGCCTCTTGATGCCCATCATGTCCCAGTTCGTCCCCGGCCTCGAGCACGGCAAAGGAATGTACTTCCTCTTCGTCAAGGCCGAGGCTCGAACCCCCGGAGGCCTTCTCGCCCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTCAAGGACCGCCCCTACGATCCTTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCCGACTCCTACCAGAGCATGTACGCTCAGTTGCTGTCCGGCCTCTGCCACCGCCTTGACGTTCTTCGCGTTGGGGCCGTTTTCGCCTCCGGGTTCATCCGCGCCATTCGCTTCCTCGAGAACAACTGGCAGCTTCTCTGCCACGATATCAGGACCGGGACACTCAACTCTTTGATCACGGATCAGGGAGTTCGCAATGTAGTCGTGTCACTCCTGCAAAAAACCGATCCCAAGCTGGGGGATTTTGTTTATGAGGAGTGTTGCAAGGGGTCATGGCATGGAATTATTCCGAGGCTTTGGCCCAATACGAAGTATGTTGATGTGATTGTAACGGGGACAATGTCACAGTACATTGGGACGCTTGATTATTATAGCGATGGGCTGCCTTTAGTGTGTACCATGTATGCTTCTTCCGAGTCCTATTTTGGCGTCAATCTTAACCCACTTTCCAAGCCTAGTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAGTTCCTTCCTGTTCAAAGAAACCACTATAACCAAGACTCTATGGACGAGCAAGAACTCGTGGATCTCACTGATGTCGAACTCGGCAAAGAATATGAGCTTGTTGTCACCACTTATGCTGGTAATTCATTCATTTTCTTTCTCTATAAACTATATCAATTATCTATAAGAAGATATAACAACATTTATAACATATATTAATTTTGAGATGAAACTTCATATATTTCTACTTAGCACCTGATTTCTCTTTTAACTAGCGTAAAAAGATTCTTGCTCCATGCCTATCTCTAACACGGGCTTGAATTCATAGGACTATATCGTTATCGAGTTGGTGACATTCTAAGAGTTGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGCCGAAAGAATGTGGTTCTGAGCATTGACTCCGACAAGACCGACGAAGTTGAGCTTCAAAATGCAGTAAAAAACTCAGTCAAGCATTTGATGCCATTCGACACAACGCTGGCGGAGTACACAAGCTATGCGAACACTTCAACAATTCCGGGACACTACGTGGTGTATTGGGAGCTAAACCATAATGGGTTAACTGCAGTGCCACCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAAGAGTCGCTGAACAGTGTATACCGACAAGGGCGGGTCTCGGACAAGTCGATTGGGCCACTAGAGATCAAGCTTGTGGAAAGTGGGACATTTGATAAGCTCATGGATTATGCCATAAGCATGGGAGCTTCTATAAACCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATTGTTAAGCTTTTGAACTCAAAAGTGGTGGCTACTTATTTCAGCCCCAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGAGCCAACAGGATTGAAGCTTAATTAATTAATTATCAAGAATTTTATGTGCCTTTTCTTTTTGTATCCATGGTAGCTGTTGTTTTTTC

mRNA sequence

CCCCTCTCTTGTCTTCCTTGTGGGACAATAAAGAAGGGAAGAACAATGCCTGAAGCACCAAACACAAGGTCTCTGGTGGAGAAAAACAAGGCCGCTCTGGAATTCATAGAGGACATCACGAGCAACGCCGGCCACGTCCAGCGCCAAGTGCTCTCCCAAATCCTCTCCGGCAACGCCTCCTCCGAGTACTTAACCCTCCACGGGCGCCCCAATTCCCCTGACACCTTCAAGTCCACCCTCCCTTTAGTCTCCTACGACCAAATCCAACCCTTCATCTCCCGCATCGCCAACGGCGACACCTCCCCCATCCTCTCCTCCTCCCCCATCTCCGAGTTCCTCACCAGCTCCGGCACCTCCGCCGGCGAGCGCAAGCTCATGCCCACCATCCACCAAGATCTCGACCGCCGCTCCCTCTTGTACAGCCTCTTGATGCCCATCATGTCCCAGTTCGTCCCCGGCCTCGAGCACGGCAAAGGAATGTACTTCCTCTTCGTCAAGGCCGAGGCTCGAACCCCCGGAGGCCTTCTCGCCCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTCAAGGACCGCCCCTACGATCCTTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCCGACTCCTACCAGAGCATGTACGCTCAGTTGCTGTCCGGCCTCTGCCACCGCCTTGACGTTCTTCGCGTTGGGGCCGTTTTCGCCTCCGGGTTCATCCGCGCCATTCGCTTCCTCGAGAACAACTGGCAGCTTCTCTGCCACGATATCAGGACCGGGACACTCAACTCTTTGATCACGGATCAGGGAGTTCGCAATGTAGTCGTGTCACTCCTGCAAAAAACCGATCCCAAGCTGGGGGATTTTGTTTATGAGGAGTGTTGCAAGGGGTCATGGCATGGAATTATTCCGAGGCTTTGGCCCAATACGAAGTATGTTGATGTGATTGTAACGGGGACAATGTCACAGTACATTGGGACGCTTGATTATTATAGCGATGGGCTGCCTTTAGTGTGTACCATGTATGCTTCTTCCGAGTCCTATTTTGGCGTCAATCTTAACCCACTTTCCAAGCCTAGTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAGTTCCTTCCTGTTCAAAGAAACCACTATAACCAAGACTCTATGGACGAGCAAGAACTCGTGGATCTCACTGATGTCGAACTCGGCAAAGAATATGAGCTTGTTGTCACCACTTATGCTGGACTATATCGTTATCGAGTTGGTGACATTCTAAGAGTTGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGCCGAAAGAATGTGGTTCTGAGCATTGACTCCGACAAGACCGACGAAGTTGAGCTTCAAAATGCAGTAAAAAACTCAGTCAAGCATTTGATGCCATTCGACACAACGCTGGCGGAGTACACAAGCTATGCGAACACTTCAACAATTCCGGGACACTACGTGGTGTATTGGGAGCTAAACCATAATGGGTTAACTGCAGTGCCACCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAAGAGTCGCTGAACAGTGTATACCGACAAGGGCGGGTCTCGGACAAGTCGATTGGGCCACTAGAGATCAAGCTTGTGGAAAGTGGGACATTTGATAAGCTCATGGATTATGCCATAAGCATGGGAGCTTCTATAAACCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATTGTTAAGCTTTTGAACTCAAAAGTGGTGGCTACTTATTTCAGCCCCAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGAGCCAACAGGATTGAAGCTTAATTAATTAATTATCAAGAATTTTATGTGCCTTTTCTTTTTGTATCCATGGTAGCTGTTGTTTTTTC

Coding sequence (CDS)

ATGCCTGAAGCACCAAACACAAGGTCTCTGGTGGAGAAAAACAAGGCCGCTCTGGAATTCATAGAGGACATCACGAGCAACGCCGGCCACGTCCAGCGCCAAGTGCTCTCCCAAATCCTCTCCGGCAACGCCTCCTCCGAGTACTTAACCCTCCACGGGCGCCCCAATTCCCCTGACACCTTCAAGTCCACCCTCCCTTTAGTCTCCTACGACCAAATCCAACCCTTCATCTCCCGCATCGCCAACGGCGACACCTCCCCCATCCTCTCCTCCTCCCCCATCTCCGAGTTCCTCACCAGCTCCGGCACCTCCGCCGGCGAGCGCAAGCTCATGCCCACCATCCACCAAGATCTCGACCGCCGCTCCCTCTTGTACAGCCTCTTGATGCCCATCATGTCCCAGTTCGTCCCCGGCCTCGAGCACGGCAAAGGAATGTACTTCCTCTTCGTCAAGGCCGAGGCTCGAACCCCCGGAGGCCTTCTCGCCCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTCAAGGACCGCCCCTACGATCCTTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCCGACTCCTACCAGAGCATGTACGCTCAGTTGCTGTCCGGCCTCTGCCACCGCCTTGACGTTCTTCGCGTTGGGGCCGTTTTCGCCTCCGGGTTCATCCGCGCCATTCGCTTCCTCGAGAACAACTGGCAGCTTCTCTGCCACGATATCAGGACCGGGACACTCAACTCTTTGATCACGGATCAGGGAGTTCGCAATGTAGTCGTGTCACTCCTGCAAAAAACCGATCCCAAGCTGGGGGATTTTGTTTATGAGGAGTGTTGCAAGGGGTCATGGCATGGAATTATTCCGAGGCTTTGGCCCAATACGAAGTATGTTGATGTGATTGTAACGGGGACAATGTCACAGTACATTGGGACGCTTGATTATTATAGCGATGGGCTGCCTTTAGTGTGTACCATGTATGCTTCTTCCGAGTCCTATTTTGGCGTCAATCTTAACCCACTTTCCAAGCCTAGTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAGTTCCTTCCTGTTCAAAGAAACCACTATAACCAAGACTCTATGGACGAGCAAGAACTCGTGGATCTCACTGATGTCGAACTCGGCAAAGAATATGAGCTTGTTGTCACCACTTATGCTGGACTATATCGTTATCGAGTTGGTGACATTCTAAGAGTTGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGCCGAAAGAATGTGGTTCTGAGCATTGACTCCGACAAGACCGACGAAGTTGAGCTTCAAAATGCAGTAAAAAACTCAGTCAAGCATTTGATGCCATTCGACACAACGCTGGCGGAGTACACAAGCTATGCGAACACTTCAACAATTCCGGGACACTACGTGGTGTATTGGGAGCTAAACCATAATGGGTTAACTGCAGTGCCACCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAAGAGTCGCTGAACAGTGTATACCGACAAGGGCGGGTCTCGGACAAGTCGATTGGGCCACTAGAGATCAAGCTTGTGGAAAGTGGGACATTTGATAAGCTCATGGATTATGCCATAAGCATGGGAGCTTCTATAAACCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATTGTTAAGCTTTTGAACTCAAAAGTGGTGGCTACTTATTTCAGCCCCAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGAGCCAACAGGATTGA

Protein sequence

MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAYFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD

Homology

[Close]

BLAST of Sed0007082 vs. NCBI nr
Match: XP_022942727.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 514/597 (86.10%), Postives = 552/597 (92.46%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SL+EKN AAL+FIEDIT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
           KAPQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSV HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 KAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. NCBI nr
Match: KAG6600750.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.2e-303
Identity = 512/597 (85.76%), Postives = 551/597 (92.29%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SL+EKN AAL+FIEDIT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
            APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+ HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 NAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. NCBI nr
Match: XP_023511762.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.5e-303
Identity = 512/597 (85.76%), Postives = 551/597 (92.29%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SL+EKN AAL+FIEDIT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
            APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+ HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 NAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. NCBI nr
Match: XP_022985796.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.6e-303
Identity = 513/597 (85.93%), Postives = 550/597 (92.13%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SLVEKN AAL+FIE IT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
            APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSV HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 NAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. NCBI nr
Match: XP_023520418.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.8e-302
Identity = 514/605 (84.96%), Postives = 557/605 (92.07%), Query Frame = 0


Query: 1   MPEAPNT-------RSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHG 60
           MPEAP T        +LV+KN A L+FIED+T+NA  VQRQVLSQILS NA+SEYL+LHG
Sbjct: 1   MPEAPKTPTSVSPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLHG 60

Query: 61  RPNSPDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPT 120
           RP SPDTFKS++PLVSYD IQPFISRIANGDTSPIL SSPISEFLTSSGTS GERKLMPT
Sbjct: 61  RP-SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180
           I ++L+RRSLLYSLLMPIM+QFVPGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK+RPYDPYTNYTSPNEAILCPD+YQS+YAQLL GLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPR 300
           LE +WQLLCHDIRTGTLNS ITDQ VR+ V+ +L++ DP+L DF++E+CCKG W GII R
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGYWKGIITR 300

Query: 301 LWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYT 360
           LWPNTKY+DVIVTGTMSQYIGTLDYYS+GLPLVCTMYASSE YFGVNLNPL KPSEVAYT
Sbjct: 301 LWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYT 360

Query: 361 LIPSMAYFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           LIPSMAYFEFLPVQR   N +S+D+Q+LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 LIPSMAYFEFLPVQRTSNNDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSV HL+PF+ TLAEYTS+ANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSHANT 480

Query: 481 STIPGHYVVYWELNHNG--LTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKL 540
           STIPGHYV+YWELN  G   T VPPSV EDCCLT+EESLNSVYRQGRV+DKSIGPLEIK+
Sbjct: 481 STIPGHYVLYWELNQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEIKV 540

Query: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQ 597
           VESGTFDKLMDYAISMGASINQYKTPRCVKF+PIV+LLNS+VV  YFSPKCPKWVAGHKQ
Sbjct: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGHKQ 600

[Close]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 1.3e-265
Identity = 456/610 (74.75%), Postives = 512/610 (83.93%), Query Frame = 0


Query: 1   MPEAP-------NTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHG 60
           MPEAP       +  SL EKNK  L+FIED+T+NA  VQR+VL +ILS NA  EYL  HG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  RPNSPD--TFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLM 120
                D  TFK  +P+V+Y+ IQP I+RIANGD S +L S+PISEFLTSSGTS GERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI ++LDRRSLLYSLLMP+M QFVPGL+ GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPN+ ILC DSYQSMY+Q+L GLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGII 300
           +FLE +W  L  DIRTGTL+S ITD  VR  V  +L K DPKL DFV  EC K SW GII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGII 300

Query: 301 PRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVA 360
            RLWPNTKYVDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNL PL KPSEV+
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPSMAYFEFLPVQRNHYNQDSM---------DEQELVDLTDVELGKEYELVVTTYAG 420
           YTLIP+MAYFEFLPV RN     S+         ++QELVDL DV+LG+EYELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDT 480
           LYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HL+PFD 
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDA 480

Query: 481 TLAEYTSYANTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDK 540
           +L+EYTSYA+TS+IPGHYV++WEL  NG T +PPSVFEDCCLT+EESLNSVYRQGRVSDK
Sbjct: 481 SLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 540

Query: 541 SIGPLEIKLVESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKC 593
           SIGPLEIK+VESGTFDKLMDYAIS+GASINQYKTPRCVKF PI++LLNS+VV +YFSPKC
Sbjct: 541 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 600

[Close]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.4e-264
Identity = 455/611 (74.47%), Postives = 516/611 (84.45%), Query Frame = 0


Query: 1   MPEAPNTRSLV-------EKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLH- 60
           MPEAP   SL        +KNK  L+ IE++TSNA  VQRQVL +IL+ NA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --GRPNSPDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKL 120
             GR +  +TFK+ +P+++Y+ I+P I+RIANGD SPILSS PISEFLTSSGTS GERKL
Sbjct: 61  LNGRTDR-ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYY 180
           MPTI ++LDRRSLLYSLLMP+MSQFVPGLE+GKGMYFLF+K+E++TPGGL ARPVLTSYY
Sbjct: 121 MPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYY 180

Query: 181 KSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRA 240
           KSSHFK+RPYDPYTNYTSPNE ILC DSYQSMY+Q+L GLC   +VLRVGAVFASGFIRA
Sbjct: 181 KSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRA 240

Query: 241 IRFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGI 300
           I+FLE +W  L  DIRTGTL+SLITD  VR  V  +L K  PKL DFV  EC K SW GI
Sbjct: 241 IKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGI 300

Query: 301 IPRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEV 360
           I RLWPNTKYVDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNL PL KPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPVQRNHYNQDSM---------DEQELVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLPV RN+   +S+         ++QELVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFD 480
           GL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HL+PFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 TTLAEYTSYANTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
            +L+EYTSYA+TS+IPGHYV++WEL  +G T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKLVESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPK 593
           KSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI++LLNS+VV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.1e-235
Identity = 405/603 (67.16%), Postives = 481/603 (79.77%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKA------ALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGR 60
           MPEAP  ++      A      ALEFIE +T+NAG VQR+VL +IL+ NA +EYL  +G 
Sbjct: 1   MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGI 60

Query: 61  PNSP---DTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLM 120
           P SP   D F+  +PLV+Y+ +QP I RIANGDTSPI S  PISEFLTSSGTS GERKLM
Sbjct: 61  PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI  +++RRSLLYSLLMP+MSQ V GL+ GK MY LFVKAE+RTPGGL ARPVLTSYY+
Sbjct: 121 PTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYR 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAI 240
           S  F DRP DPYT+YTSP+EAILC DSYQSMYAQLL GL HR DVLRVGAVFASGF+RAI
Sbjct: 181 SRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAI 240

Query: 241 RFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGII 300
            FLE +W  LCHDIRTG L+  ITD+ VR+ V  +L + DP L D + +EC + SW GII
Sbjct: 241 HFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRVL-RADPALADAIEDECARASWEGII 300

Query: 301 PRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVA 360
            RLWP TKY+DVIVTGTMSQYI TL++Y  GLPL CTMYASSE YFG+NLNP+ KPS+VA
Sbjct: 301 RRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVA 360

Query: 361 YTLIPSMAYFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           YTLIP+M Y+EFLPV  N+   ++   ++LVDL DV+LG EYELVVTTY+GLYRYRVGD+
Sbjct: 361 YTLIPTMCYYEFLPVNCNNATAEA-SHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDV 420

Query: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYA 480
           LRVAGFKNKAP F+F+ R+NV LS+DSDKTDE EL  AV  +V+HL PF  +L EYTSYA
Sbjct: 421 LRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYA 480

Query: 481 NTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKL 540
           + +TIPGHYV++WEL   G TAVP SVFE+CCL++EE+LNSVYRQGR  D+SIGPLEI++
Sbjct: 481 DAATIPGHYVLFWEL-RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRV 540

Query: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQ 595
           V  GTFDKLMDYAIS GASINQYK PRCV+  P+V+LL+++V   YFSPKCPKW  G+KQ
Sbjct: 541 VAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQ 600

[Close]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 3.6e-223
Identity = 387/600 (64.50%), Postives = 463/600 (77.17%), Query Frame = 0


Query: 2   PEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHG----RPNS 61
           P A    ++  +++  LE IE +T NAG  QR+VL +IL+ NA +EYL   G     P +
Sbjct: 11  PAAAAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGA 70

Query: 62  PDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQD 121
            + F+   PLV+Y+ I P + RIANGDTSPILS  P+SEFLTSSGTS GERKLMPTI ++
Sbjct: 71  DEAFRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEE 130

Query: 122 LDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKD 181
           ++RRS LYSLLMP+MS+ VPGL+ GK MY  FVK+E RTPGGL ARPVLTS+Y+S +F +
Sbjct: 131 MERRSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLE 190

Query: 182 RPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENN 241
           RP+DPYT YTSP+EA+LC D+YQSMYAQL+ GL HR DVLRVGAVFASGF+RAIRFLE +
Sbjct: 191 RPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKH 250

Query: 242 WQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPN 301
           W  LC DIR G L+  +TD  VR  V  +L+  DP L D +  EC + SW GII R+WP+
Sbjct: 251 WPSLCRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPS 310

Query: 302 TKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPS 361
           TKY+DVIVTG M+QYI TL++Y  GLPL CTMYASSE YFG+NLNP+ KPSEVAYTLIP+
Sbjct: 311 TKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPT 370

Query: 362 MAYFEFLPVQ--RNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVA 421
           M YFEFLPV    N       D + LVDL DV+LG EYELVVTTY+GLYRYRVGD+LRVA
Sbjct: 371 MCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVA 430

Query: 422 GFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANT-S 481
           GFKN AP F F+ RKNV LSIDSDKTDE EL  AV  +V+HL PF  +L EYTSYA+T +
Sbjct: 431 GFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTAT 490

Query: 482 TIPGHYVVYWEL-NHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVE 541
           TIPGHYV++WEL +  G T VP SVFEDCCL +EE LNSVYRQ R +D+SIGPLEI++V 
Sbjct: 491 TIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVA 550

Query: 542 SGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVA-GHKQW 593
            GTFDKLMDYA+S GASINQYK PRCV+  P+V+LL+ +V ATYFSPKCPKW A G+KQW
Sbjct: 551 DGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQW 610

[Close]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 8.2e-212
Identity = 372/580 (64.14%), Postives = 449/580 (77.41%), Query Frame = 0


Query: 12  EKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYL---TLHGRPNSPDTFKSTLPLV 71
           EK+  AL FIE++T NA  VQ  +L++IL+ NA +EYL    L G  +  DTFK+ +P++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDR-DTFKTKIPVI 76

Query: 72  SYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRRSLLYSLL 131
           +Y+ +QP I RIA+GD SPILS+ PISEFLTSSGTSAGERKLMPTI ++LDRR LLYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 132 MPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTS 191
           MP+M+ +VPGL+ GKGMYFLFVK+E +TPGGL ARPVLTSYYKS HF+ RPYDPY  YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 192 PNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLLCHDIRTG 251
           PNEAILCPDS+QSMY Q+L GL  RL VLRVGAVFASG +RAIRFL+ +W    HDI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 252 TLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKYVDVIVTGT 311
            L+S ITD  +R  +  +L K DP L +F+  EC   +W  II R+WPNTKY+DVIVTG 
Sbjct: 257 CLDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGA 316

Query: 312 MSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAYFEFLPVQR 371
           M+QYI TL+YYS GLP+ CTMYASSE YFG+NLNP+SKPSEV+YT++P+MAYFEF+P   
Sbjct: 317 MAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIP--- 376

Query: 372 NHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFIC 431
                  +   + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ 
Sbjct: 377 -------LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 432 RKNVVLSIDSDKTDEVELQNAVKN--SVKHLMPFDTTLAEYTSYANTSTIPGHYVVYWE- 491
           RKNV+LSIDSDKTDE ELQ AV+N  S+ H     + +AEYTSYA+TSTIPGHYV+YWE 
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILH-EECGSRVAEYTSYADTSTIPGHYVLYWEL 496

Query: 492 LNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDKLMDYAI 551
           L  +G           CCL MEESLNSVYRQ RV+D S+GPLEI++V +GTF++LMDYAI
Sbjct: 497 LVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAI 556

Query: 552 SMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKW 586
           S GASINQYK PRCV F PIV+LL+S+VV+ +FSP  P W
Sbjct: 557 SRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of Sed0007082 vs. ExPASy TrEMBL
Match: A0A6J1FR29 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447677 PE=3 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 514/597 (86.10%), Postives = 552/597 (92.46%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SL+EKN AAL+FIEDIT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
           KAPQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSV HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 KAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. ExPASy TrEMBL
Match: A0A6J1J981 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483735 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 513/597 (85.93%), Postives = 550/597 (92.13%), Query Frame = 0


Query: 1   MPEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDT 60
           MPEAPN  SLVEKN AAL+FIE IT+NA HVQRQVLSQILS NASSEYLTLH RP SPDT
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDR 120
           FKS++PL+SYD IQPFI+RIANGDTSPIL SSPISEFLTSSGTS GERKLMPTIH++LDR
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180
           RSLLYSLLMP+M+QF+PGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY
Sbjct: 121 RSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQL 240
           DPYTNYTSPNEAILC DSYQSMYAQ+L GLCHRLDVLRVGAVFASGFIRAIRFLE +WQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTK 300
           LCHDIR+GTLNS ITDQ VR  V++S+L++ DP+LGDF+  ECCKGSW GII RLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYS+GLPLVC+MYASSE YFG+NLNPL  P+EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           YFEFLP+Q NH +     E +LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 361 YFEFLPLQTNHNH-----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 480
            APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSV HL+PFD TL +YTSYANTSTIPGH
Sbjct: 421 NAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGH 480

Query: 481 YVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 540
           YVVYWE+N  G T VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIK+VE GTFDK
Sbjct: 481 YVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDK 540

Query: 541 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           LMDYAI+MGASINQYKTPRCVKFEPIV+LLNS+VV +YFSPKCPKWVAGHKQW QQD
Sbjct: 541 LMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of Sed0007082 vs. ExPASy TrEMBL
Match: A0A6J1JAP2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483268 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 5.2e-302
Identity = 513/605 (84.79%), Postives = 556/605 (91.90%), Query Frame = 0


Query: 1   MPEAPNTR-------SLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHG 60
           MPEAP T        +LV+KN A L+FIED+T+NA  VQRQVLSQILS NA+SEYL+LHG
Sbjct: 1   MPEAPKTATSVSPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLHG 60

Query: 61  RPNSPDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPT 120
           RP SPDTFKS++PLVSYD IQPFISRIANGDTSPIL SSPISEFLTSSGTS GERKLMPT
Sbjct: 61  RP-SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180
           I ++L+RRSLLYSLLMPIM+QFVPGLE GKGMYFLFVKAEARTPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK+RPYDPYTNYTSPNEAILCPD+YQS+YAQLL GLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPR 300
           LE +WQLLCHDIRTGTLNS ITDQ VR+ V+ +L++ DP+L DF++E+CCKG W GII R
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGFWKGIITR 300

Query: 301 LWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYT 360
           LWPNTKY+DVIVTGTMSQYIGTLDYYS+GLPLVCTMYASSE YFGVNLNPL KPSEVAYT
Sbjct: 301 LWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYT 360

Query: 361 LIPSMAYFEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           LIPSMAYFEFLPVQR   N +S+D+Q+LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 LIPSMAYFEFLPVQRTSNNDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK SV HL+PF+ TLAEYTS+ANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAEYTSHANT 480

Query: 481 STIPGHYVVYWELNHNG--LTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKL 540
           STIPGHYV+YWELN  G   T VPPSV EDCCLT+EESLNSVYRQGRV+DKSIGPLEIK+
Sbjct: 481 STIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEIKV 540

Query: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQ 597
           VESGTFDKLMDYAISMGASINQYKTPRCVKF+PIV+LLNS+VV  YFSPKCPKWVAGHKQ
Sbjct: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGHKQ 600

[Close]

BLAST of Sed0007082 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.8e-302
Identity = 509/599 (84.97%), Postives = 556/599 (92.82%), Query Frame = 0


Query: 2   PEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDTF 61
           P  PN   LV+KNKA L+FIED+T+NA  VQRQVLSQILS N++SEYLTL+GRP SPDTF
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP-SPDTF 66

Query: 62  KSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRR 121
           K+++PLVSYDQIQPF+SRIANGD SPIL SSPISEFLTSSGTS GERKLMPTI ++L+RR
Sbjct: 67  KTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERR 126

Query: 122 SLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 181
           SLLYSLLMP+MSQFVPGLE GKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYD
Sbjct: 127 SLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYD 186

Query: 182 PYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLL 241
           PYTNYTSPNEAILCPDSYQSMYAQLL GLCHRLDVLRVGAVFASGFIRAIRFLE +WQLL
Sbjct: 187 PYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 246

Query: 242 CHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKY 301
           CHDIRTGTLNS ITDQ VR+ V++S+L++ DP+L D+++ ECCKGSW GII RLWPNTKY
Sbjct: 247 CHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKY 306

Query: 302 VDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAY 361
           VDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNLNPL KPSEVAYTLIPSMAY
Sbjct: 307 VDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAY 366

Query: 362 FEFLPVQRNHYNQD-SMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 421
           FEFLP++R+H N D S+DEQ+LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 367 FEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 426

Query: 422 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 481
           KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+ HL+PF+ TLAEYTSYANTSTIPGH
Sbjct: 427 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGH 486

Query: 482 YVVYWELN--HNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTF 541
           YV+YWE+N      T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIK+VE+GTF
Sbjct: 487 YVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTF 546

Query: 542 DKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           DKLMDYAISMGASINQYKTPRCVKF+PIV+LLNS+VV +YFSPKCPKWV GHKQWSQQD
Sbjct: 547 DKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of Sed0007082 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.8e-302
Identity = 509/599 (84.97%), Postives = 556/599 (92.82%), Query Frame = 0


Query: 2   PEAPNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPDTF 61
           P  PN   LV+KNKA L+FIED+T+NA  VQRQVLSQILS N++SEYLTL+GRP SPDTF
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP-SPDTF 66

Query: 62  KSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRR 121
           K+++PLVSYDQIQPF+SRIANGD SPIL SSPISEFLTSSGTS GERKLMPTI ++L+RR
Sbjct: 67  KTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERR 126

Query: 122 SLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 181
           SLLYSLLMP+MSQFVPGLE GKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYD
Sbjct: 127 SLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYD 186

Query: 182 PYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLL 241
           PYTNYTSPNEAILCPDSYQSMYAQLL GLCHRLDVLRVGAVFASGFIRAIRFLE +WQLL
Sbjct: 187 PYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 246

Query: 242 CHDIRTGTLNSLITDQGVRN-VVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKY 301
           CHDIRTGTLNS ITDQ VR+ V++S+L++ DP+L D+++ ECCKGSW GII RLWPNTKY
Sbjct: 247 CHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKY 306

Query: 302 VDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAY 361
           VDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNLNPL KPSEVAYTLIPSMAY
Sbjct: 307 VDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAY 366

Query: 362 FEFLPVQRNHYNQD-SMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 421
           FEFLP++R+H N D S+DEQ+LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN
Sbjct: 367 FEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 426

Query: 422 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGH 481
           KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+ HL+PF+ TLAEYTSYANTSTIPGH
Sbjct: 427 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGH 486

Query: 482 YVVYWELN--HNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTF 541
           YV+YWE+N      T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIK+VE+GTF
Sbjct: 487 YVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTF 546

Query: 542 DKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKWVAGHKQWSQQD 597
           DKLMDYAISMGASINQYKTPRCVKF+PIV+LLNS+VV +YFSPKCPKWV GHKQWSQQD
Sbjct: 547 DKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of Sed0007082 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 916.8 bits (2368), Expect = 9.2e-267
Identity = 456/610 (74.75%), Postives = 512/610 (83.93%), Query Frame = 0


Query: 1   MPEAP-------NTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHG 60
           MPEAP       +  SL EKNK  L+FIED+T+NA  VQR+VL +ILS NA  EYL  HG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  RPNSPD--TFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLM 120
                D  TFK  +P+V+Y+ IQP I+RIANGD S +L S+PISEFLTSSGTS GERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI ++LDRRSLLYSLLMP+M QFVPGL+ GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPN+ ILC DSYQSMY+Q+L GLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGII 300
           +FLE +W  L  DIRTGTL+S ITD  VR  V  +L K DPKL DFV  EC K SW GII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGII 300

Query: 301 PRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVA 360
            RLWPNTKYVDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNL PL KPSEV+
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPSMAYFEFLPVQRNHYNQDSM---------DEQELVDLTDVELGKEYELVVTTYAG 420
           YTLIP+MAYFEFLPV RN     S+         ++QELVDL DV+LG+EYELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDT 480
           LYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HL+PFD 
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDA 480

Query: 481 TLAEYTSYANTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDK 540
           +L+EYTSYA+TS+IPGHYV++WEL  NG T +PPSVFEDCCLT+EESLNSVYRQGRVSDK
Sbjct: 481 SLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 540

Query: 541 SIGPLEIKLVESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKC 593
           SIGPLEIK+VESGTFDKLMDYAIS+GASINQYKTPRCVKF PI++LLNS+VV +YFSPKC
Sbjct: 541 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 600

[Close]

BLAST of Sed0007082 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 913.3 bits (2359), Expect = 1.0e-265
Identity = 455/611 (74.47%), Postives = 516/611 (84.45%), Query Frame = 0


Query: 1   MPEAPNTRSLV-------EKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLH- 60
           MPEAP   SL        +KNK  L+ IE++TSNA  VQRQVL +IL+ NA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --GRPNSPDTFKSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKL 120
             GR +  +TFK+ +P+++Y+ I+P I+RIANGD SPILSS PISEFLTSSGTS GERKL
Sbjct: 61  LNGRTDR-ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKL 120

Query: 121 MPTIHQDLDRRSLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYY 180
           MPTI ++LDRRSLLYSLLMP+MSQFVPGLE+GKGMYFLF+K+E++TPGGL ARPVLTSYY
Sbjct: 121 MPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYY 180

Query: 181 KSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRA 240
           KSSHFK+RPYDPYTNYTSPNE ILC DSYQSMY+Q+L GLC   +VLRVGAVFASGFIRA
Sbjct: 181 KSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRA 240

Query: 241 IRFLENNWQLLCHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGI 300
           I+FLE +W  L  DIRTGTL+SLITD  VR  V  +L K  PKL DFV  EC K SW GI
Sbjct: 241 IKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGI 300

Query: 301 IPRLWPNTKYVDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEV 360
           I RLWPNTKYVDVIVTGTMSQYI TLDYYS+GLPLVCTMYASSE YFGVNL PL KPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPVQRNHYNQDSM---------DEQELVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLPV RN+   +S+         ++QELVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFD 480
           GL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HL+PFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 TTLAEYTSYANTSTIPGHYVVYWELNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
            +L+EYTSYA+TS+IPGHYV++WEL  +G T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKLVESGTFDKLMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPK 593
           KSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI++LLNS+VV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of Sed0007082 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 748.0 bits (1930), Expect = 5.7e-216
Identity = 371/582 (63.75%), Postives = 453/582 (77.84%), Query Frame = 0


Query: 12  EKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPD--TFKSTLPLVS 71
           EK+  AL+FIE++T N   VQ +VL +IL+ N+++EYL         D  TFKS +P+V+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 72  YDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRRSLLYSLLM 131
           Y+ ++P I RI+NGD SPILSS PI+EFLTSSGTSAGERKLMPTI +DLDRR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 132 PIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSP 191
           P+M+ +VPGL+ GKG+YFLFVK+E++T GGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 192 NEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLLCHDIRTGT 251
           NEAILC DS QSMYAQ+L GL  R +VLR+GAVFASG +RAI FL+NNW+ L  DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 252 LNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKYVDVIVTGTM 311
           L+S I D  ++N +  +L K D +L +F+   C + +W GII ++WPNTKY+DVIVTG M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 312 SQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAYFEFLPVQRN 371
           +QYI TL+YYS GLP+ CTMYASSESYFG+NL P+ KPSEV+YT++P+MAYFEFLP   N
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--HN 377

Query: 372 HYN----QDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 431
           H      + S+DE  LV+L +VE+GKEYELV+TTYAGLYRYRVGDILRV GF N APQF 
Sbjct: 378 HDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFK 437

Query: 432 FICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGHYVVYWE 491
           FI RKNV+LS++SDKTDE ELQ AV+N+ +      T + EYTSYA T TIPGHYV+YWE
Sbjct: 438 FIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWE 497

Query: 492 L--NHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDKLMDY 551
           L         +   V   CCL MEESLNSVYRQ RV+DKSIGPLEI++V +GTF++LMDY
Sbjct: 498 LLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDY 557

Query: 552 AISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKW 586
           AIS GASINQYK PRCV F PI++LL+S+VV+ +FSP  P W
Sbjct: 558 AISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 597

[Close]

BLAST of Sed0007082 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 738.0 bits (1904), Expect = 5.9e-213
Identity = 372/580 (64.14%), Postives = 449/580 (77.41%), Query Frame = 0


Query: 12  EKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYL---TLHGRPNSPDTFKSTLPLV 71
           EK+  AL FIE++T NA  VQ  +L++IL+ NA +EYL    L G  +  DTFK+ +P++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDR-DTFKTKIPVI 76

Query: 72  SYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRRSLLYSLL 131
           +Y+ +QP I RIA+GD SPILS+ PISEFLTSSGTSAGERKLMPTI ++LDRR LLYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 132 MPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTS 191
           MP+M+ +VPGL+ GKGMYFLFVK+E +TPGGL ARPVLTSYYKS HF+ RPYDPY  YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 192 PNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLLCHDIRTG 251
           PNEAILCPDS+QSMY Q+L GL  RL VLRVGAVFASG +RAIRFL+ +W    HDI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 252 TLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCKGSWHGIIPRLWPNTKYVDVIVTGT 311
            L+S ITD  +R  +  +L K DP L +F+  EC   +W  II R+WPNTKY+DVIVTG 
Sbjct: 257 CLDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGA 316

Query: 312 MSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAYFEFLPVQR 371
           M+QYI TL+YYS GLP+ CTMYASSE YFG+NLNP+SKPSEV+YT++P+MAYFEF+P   
Sbjct: 317 MAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIP--- 376

Query: 372 NHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFIC 431
                  +   + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ 
Sbjct: 377 -------LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 432 RKNVVLSIDSDKTDEVELQNAVKN--SVKHLMPFDTTLAEYTSYANTSTIPGHYVVYWE- 491
           RKNV+LSIDSDKTDE ELQ AV+N  S+ H     + +AEYTSYA+TSTIPGHYV+YWE 
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILH-EECGSRVAEYTSYADTSTIPGHYVLYWEL 496

Query: 492 LNHNGLTAVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDKLMDYAI 551
           L  +G           CCL MEESLNSVYRQ RV+D S+GPLEI++V +GTF++LMDYAI
Sbjct: 497 LVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAI 556

Query: 552 SMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKW 586
           S GASINQYK PRCV F PIV+LL+S+VV+ +FSP  P W
Sbjct: 557 SRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of Sed0007082 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 731.1 bits (1886), Expect = 7.2e-211
Identity = 368/586 (62.80%), Postives = 450/586 (76.79%), Query Frame = 0


Query: 4   APNTRSLVEKNKAALEFIEDITSNAGHVQRQVLSQILSGNASSEYLTLHGRPNSPD--TF 63
           +P   S   K+  AL FIE++T N   VQ++V+ +ILS N+ +EYL   G     D  TF
Sbjct: 9   SPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTF 68

Query: 64  KSTLPLVSYDQIQPFISRIANGDTSPILSSSPISEFLTSSGTSAGERKLMPTIHQDLDRR 123
           K+ +P+V YD ++P I RIANGD S ILSS PI+EFLTSSGTSAGERKLMPTI +D+DRR
Sbjct: 69  KTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRR 128

Query: 124 SLLYSLLMPIMSQFVPGLEHGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 183
            LLYSLLMP+M+ +VPGL+ GK +YFLFVK E++TPGGL ARPVLTSYYKS  FK RP D
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPND 188

Query: 184 PYTNYTSPNEAILCPDSYQSMYAQLLSGLCHRLDVLRVGAVFASGFIRAIRFLENNWQLL 243
           PY  YTSPNEAILCPDS QSMY Q+L GL  R +VLR+GAVFASG +RAI FL+ NW+ L
Sbjct: 189 PYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKEL 248

Query: 244 CHDIRTGTLNSLITDQGVRNVVVSLLQKTDPKLGDFVYEECCK-GSWHGIIPRLWPNTKY 303
             DI TGTL+S I+D  ++  +  +L K D +L DF+   C +  SW GII ++WPNTKY
Sbjct: 249 ADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKY 308

Query: 304 VDVIVTGTMSQYIGTLDYYSDGLPLVCTMYASSESYFGVNLNPLSKPSEVAYTLIPSMAY 363
           +DVIVTG M+QYI  L+YYS GLP+ CTMYASSESYFG+NL P+ KPSEV+YT++P+MAY
Sbjct: 309 LDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAY 368

Query: 364 FEFLPVQRNHYNQDSMDEQELVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 423
           FEFLP     +++   ++ ELV+L DVE+GKEYELV+TTYAGL RYRVGDIL+V GF N 
Sbjct: 369 FEFLP-----HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNS 428

Query: 424 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVKHLMPFDTTLAEYTSYANTSTIPGHY 483
           APQF F+ RKNV+LSI+SDKTDE ELQ+AV+N+   L    T + EYTSYA T TIPGHY
Sbjct: 429 APQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHY 488

Query: 484 VVYWELNHNGLTAVP-PSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKLVESGTFDK 543
           V+YWEL     T  P   V   CCL MEESLNSVYRQ RV+DKSIGPLEI++V++GTF++
Sbjct: 489 VIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEE 548

Query: 544 LMDYAISMGASINQYKTPRCVKFEPIVKLLNSKVVATYFSPKCPKW 586
           LMDYAIS GASINQYK PRCV F PI++LL+S+VV+T+FSP  P W
Sbjct: 549 LMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 589

The following BLAST results are available for this feature:

BLAST of Sed0007082 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022942727.1	2.3e-304	86.10	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
KAG6600750.1	1.2e-303	85.76	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023511762.1	1.5e-303	85.76	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985796.1	2.6e-303	85.93	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima]	[more]
XP_023520418.1	2.8e-302	84.96	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0007082 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	1.3e-265	74.75	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	1.4e-264	74.47	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	1.1e-235	67.16	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	3.6e-223	64.50	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	8.2e-212	64.14	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Sed0007082 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FR29	1.1e-304	86.10	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J981	1.2e-303	85.93	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1JAP2	5.2e-302	84.79	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A5D3BYD3	6.8e-302	84.97	Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BTN3	6.8e-302	84.97	indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493...	[more]

BLAST of Sed0007082 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	9.2e-267	74.75	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	1.0e-265	74.47	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	5.7e-216	63.75	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	5.9e-213	64.14	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	7.2e-211	62.80	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 21..562 e-value: 2.8E-191 score: 636.9
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 7..593
None	No IPR available	PANTHER	PTHR31901:SF70	BNAC03G14570D PROTEIN	coord: 7..593

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0007082.1	Sed0007082.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity