Sed0007014 (gene) Chayote v1

Overview


Name	Sed0007014
Type	gene
Organism	Sechium edule (Chayote v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	LG01: 5088941 .. 5090813 (+)
RNA-Seq Expression	Sed0007014
Synteny	Sed0007014

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAATGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGGTGACCCTTTTCCCTAAAACACAATTTTTAAATCTTCTTCTTTTTTTAGATGTGGGATTTAGTTAATTCTAAAGATATTGTATTTCTGTTGTAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAGTATGTGAGAAGATTGTCTTTTTTTAATTATGTGATCCCAAGAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTATGTAGGAAAAACATAATTCTAAGCCTGGATGCTGAGAAAACGGAGGAGGGTGAGCTTCATAATGGTGTACATGAAGCTGCTCGTCTTTTAAGTCCATTTGGAGCTGCTATTGTTGACTACACAAGTTATGGAAATAACACAACAATTCCCGGACACTATGTGTTGTATTGGGAAATTCGTCTTGAGGAAGAAAGCTCCATGATTTCATCTTCAATCTATGAAGATTGTTGCCTTGCTATTGAAGAGTTGTTGAATGTCCGGTATCGTCAAGGAAGATGTCAAGAAAAAAGTATAAACGCGCTAGAGATTAGAATTGTAGAGAATGGAACTTTTGAGAAACTGATGGAGCGAGCAGTGAGTCAAGGAGTATCAATTAGTCAATACAAAACACCTCGAAGCATAAAGTCTACACAGACCATCGAACTTTTAGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATACCCAAAATGGGATCCCAATAGTAGACACCATCTTTTTTCATCCTAG

mRNA sequence

ATGTCCCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAATGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTATGTAGGAAAAACATAATTCTAAGCCTGGATGCTGAGAAAACGGAGGAGGGTGAGCTTCATAATGGTGTACATGAAGCTGCTCGTCTTTTAAGTCCATTTGGAGCTGCTATTGTTGACTACACAAGTTATGGAAATAACACAACAATTCCCGGACACTATGTGTTGTATTGGGAAATTCGTCTTGAGGAAGAAAGCTCCATGATTTCATCTTCAATCTATGAAGATTGTTGCCTTGCTATTGAAGAGTTGTTGAATGTCCGGTATCGTCAAGGAAGATGTCAAGAAAAAAGTATAAACGCGCTAGAGATTAGAATTGTAGAGAATGGAACTTTTGAGAAACTGATGGAGCGAGCAGTGAGTCAAGGAGTATCAATTAGTCAATACAAAACACCTCGAAGCATAAAGTCTACACAGACCATCGAACTTTTAGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATACCCAAAATGGGATCCCAATAGTAGACACCATCTTTTTTCATCCTAG

Coding sequence (CDS)

ATGTCCCATGAGGCAGAAAACCTGCTAGAGGAGAAAGACAAGCAAGCGTTGCAACATATTGAAGATATAACGAGCAATGCCGGCGAAATCCAACGACAAACTCTGGCCGAAATTCTGGCAACAAATGCTGATGTTGAGTACTTGCAGCAACATGGTCTTAATGGATCTACTGATGCTTCAACTTTCAAGAACTTTATACCGGTCATATCTTACGAACAATTAAAACCTTACATAGATCGTATTGCAAATGGTGACAATTCTCCAATCCTATGCACAGAACCCATTACAGAGTTCTTTATCAGCACTGGAACAACCGGGGGAGAACAAAAGTTAATTCCAATATTTGAAAAAGAAATGAAACAGTTGTTTCCAAATTCCCACAAAGGAAAAGAAATGTTCTTCTGTTTCACCAAGGCTGAGTCTACAACTAAAGCTGGAATTATTGCTTATTCTACATCAACAAGTTTGTGCAAGAGATCACACTTCAACAATATACAAAGTGGTGGTAACACGAGTCCGATCGACGTCACTTTATGCACAGACACATACCAGAGCACATATTCTCAGTTGCTTTGTGGGCTCTATCAAAATGAGAAGGTTCATAAATTGGGTGCTCTTTTTGTTTCAACTTTAATTTATGTTCTTAAGTTCCTCGAAAACCATTGGATTCTCTTGGCTAATGATATTCGAACCGGAACCATAGATCCAAAAATTATAGACTCATCCGTGAGAGAATCGGTTATGAACATTCTTAAACCGAATCCACAACTTGCAGATTTCATTGAAATTGAATGTAACAAAGGGTCATGGGAAGGGATCATTCCAAGATTGTGGCCAAACGCCAATTACATTAAAGCTATTGCTTCAGGAAGCATGTCACAATACGTTCCCCTGATTAATTACTATAGTAATAACCTCCCTCTTATTTCAGATCACTATGGTTCTTCAGAATGCTTCCTTGGCTTGAACTTAGATCCATTATGCAAACCTGAAGAAATCAGCTACACTTTAGTCCCCGACATGGCATATTTCGAGTTCTTGCCAATTGAAAGAGAAAAGGCTAATGGAGAAGCAAGGCAGGAATTAGTTGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTGGTCATCACCACTTATGGTGGCCTCTACCGCTACTGCATGGGTGACATCCTTCGCGTGAAAGGGTTTAAAAACAAAGCCCCAAACTTTAGTTTTGTATGTAGGAAAAACATAATTCTAAGCCTGGATGCTGAGAAAACGGAGGAGGGTGAGCTTCATAATGGTGTACATGAAGCTGCTCGTCTTTTAAGTCCATTTGGAGCTGCTATTGTTGACTACACAAGTTATGGAAATAACACAACAATTCCCGGACACTATGTGTTGTATTGGGAAATTCGTCTTGAGGAAGAAAGCTCCATGATTTCATCTTCAATCTATGAAGATTGTTGCCTTGCTATTGAAGAGTTGTTGAATGTCCGGTATCGTCAAGGAAGATGTCAAGAAAAAAGTATAAACGCGCTAGAGATTAGAATTGTAGAGAATGGAACTTTTGAGAAACTGATGGAGCGAGCAGTGAGTCAAGGAGTATCAATTAGTCAATACAAAACACCTCGAAGCATAAAGTCTACACAGACCATCGAACTTTTAGATGCAAGAGTTTTGTCCAACCATTTTAGCCAAAAATACCCAAAATGGGATCCCAATAGTAGACACCATCTTTTTTCATCCTAG

Protein sequence

MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEMKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRHHLFSS

Homology

[Close]

BLAST of Sed0007014 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 819.7 bits (2116), Expect = 1.6e-233
Identity = 394/580 (67.93%), Postives = 462/580 (79.66%), Query Frame = 0


Query: 9   LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 68
           L+EKDKQALQ +ED+T  A EIQR+ L EIL+TNA VEYLQQHGLNGSTDASTFKN IP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE---------- 128
           +SYE LKPYIDRI NGD+SPILC  PITEF+ STGT+GGEQKLIP  E+E          
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 ----MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGGNTSP 188
               MKQLFP  H GKEMFF F K ES TKAGI+A S STS  K  H NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 248
            D+  CTD YQS Y QLLCGL QN+KV ++G+LF STLIY+ KFLENHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 308
           I+ KI+DS VR+SVMNILKP+PQLADFIE EC+KG+WEGI+ RLWPN  +I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 368
            Y+P +NYY  +LPLI+  Y SSECF G+NLDPLCKPEEISYT +P +AYFEFLP++R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNI 428
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FV RKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 ILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEES 488
           ILSLD EKT+E ELHN V++   LLS FGA +VDYTSY +++T PGHYVLYWEI+ +EE 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 SMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSI 548
           S+I SSIYEDCCLAIEE L+  YR+ R  +K IN LEI+IVE  TF+KLMER V QG SI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 SQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRH 574
           SQYKTPR +KSTQ IE LD++V++N+FS+KYPKWDP   H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Sed0007014 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.0 bits (2083), Expect = 1.1e-229
Identity = 400/593 (67.45%), Postives = 478/593 (80.61%), Query Frame = 0


Query: 1   MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 60
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE-- 120
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 ------------MKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 180
                       MKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 240
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 300
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 420
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRYCMGDILRV GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSLD EKT+E ELH+ V EAARLLSPFGAAI DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLME 540
           WEI ++   S I SSIYEDCC AIEELLN  YR  R  EK IN LEI+IV+ GTFEKLM 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRHHLFS 578
           +A+++G S+SQYKTPR + ST  IELLD+ V++ +FS++ PK D N+ H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

[Close]

BLAST of Sed0007014 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 798.1 bits (2060), Expect = 5.0e-227
Identity = 398/593 (67.12%), Postives = 475/593 (80.10%), Query Frame = 0


Query: 1   MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 60
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE-- 120
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 ------------MKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 180
                       MKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 240
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 300
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 420
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRY MGDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSLD EKT+E ELH+ V EAARLLSPFGAAI DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLME 540
           WEI ++   S I SSIYEDCC AIEELLN  YR  R  EK IN LEI+IV+ GTFEKLM 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRHHLFS 578
           +A+++G S+SQYKTPR + ST  IELLD+ V+  +FS+K PK D  + H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0007014 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 777.3 bits (2006), Expect = 9.1e-221
Identity = 381/578 (65.92%), Postives = 457/578 (79.07%), Query Frame = 0


Query: 5   AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 64
           ++++L+ KDKQ L HIED TSNA EIQR+ + EIL+ NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------ 124
            IPV+SYEQLKPYIDRIA+GD+SPILC + IT+F ISTGT+GG QKLIP+F+ E      
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 --------MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGG 184
                   MKQLFP    GKEMFF FT+AE+ TK G++A   S+ L K S F++++    
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRA 181

Query: 185 NTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDI 244
            TSPID+  CTD YQS Y QLLCGL QN+ V ++GALF STLIY+ KFLENHW LL NDI
Sbjct: 182 YTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDI 241

Query: 245 RTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIAS 304
           RTGT+D KI D  VRESVM++LKPNP+LADFIE EC+KG+WEGI+ RLWPN  YI  + +
Sbjct: 242 RTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVT 301

Query: 305 GSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPI 364
           GSMSQY+P +N+Y    P++S  Y SSECF G+NLDPLCKPEEISYT +P MAYFEFLP+
Sbjct: 302 GSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPV 361

Query: 365 EREKA-NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFV 424
           +R  + NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYCMGDILRV GFKNK PNFSF 
Sbjct: 362 DRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFA 421

Query: 425 CRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIR 484
           CRKN+ILSLD EKT+E +LH+ V EA+ LLSP GA IVDYTSY +++T PGHYVLYWEI+
Sbjct: 422 CRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQ 481

Query: 485 LEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVS 544
             EE+S I S++YEDCCLA+EE L+  YRQ R   K IN LEI+IVE GTFEKLME+AV 
Sbjct: 482 CREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVD 541

Query: 545 QGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPK 567
           +G SISQYKTPR +KSTQ IELLD++V+SN+FSQ+YPK
Sbjct: 542 RGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0007014 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 769.2 bits (1985), Expect = 2.5e-218
Identity = 390/597 (65.33%), Postives = 467/597 (78.22%), Query Frame = 0


Query: 1   MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 60
           MSH  E+ L+EKDK+ALQHIED+TS A EIQRQ L EIL+TNA+VEYLQQHGL+GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE-- 120
           TFK  IP+ISYEQL PY+  IA+ D+SPILC+ PIT FF S+GT+GGE KLIP++E+E  
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 ------------MKQLFPNSH--KGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 180
                       MKQLFPN +  KGK M F F K E  TKAGII  S  TSL KRS  +N
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRS--SN 180

Query: 181 IQS---GGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENH 240
           ++S   G NTSP D+ LCTD++QS Y QLLCGLYQN+ V +LGALF STLI+V KFLENH
Sbjct: 181 LKSKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENH 240

Query: 241 WILLANDIRTGTIDPKIIDSSVRESVMNI-LKPNPQLADFIEIECNKGSWEGIIPRLWPN 300
           W++LANDI TG+I+PKI + SVRESVM I +KPNP++AD IE EC+KGSWEGII RLWPN
Sbjct: 241 WVVLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPN 300

Query: 301 ANYIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPD 360
             YI AI +GSMSQY+PL+N+Y+NNLP++SDHYGSSECFLGLNLDPLC P EISYTL+P 
Sbjct: 301 TKYINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPT 360

Query: 361 MAYFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKN 420
           MAYFEFLPI+R   NGE  Q+LVDLVDVKLGQEYELVITT+ GLYRY MGDI+RV GF  
Sbjct: 361 MAYFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTK 420

Query: 421 KAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGH 480
           KAPNFSFVCRKN++LSL+ EKT+E  LH  V E   +L PFGA IVDYTSY +++TIPGH
Sbjct: 421 KAPNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGH 480

Query: 481 YVLYWEIRLEEE-----SSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVE 540
           YVLY+E+  +E      S+ I SS+YEDCC AIEE LN  YR  R  EK I+ LEI+IV+
Sbjct: 481 YVLYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVK 540

Query: 541 NGTFEKLMERAVSQGVSISQYKTPRSIKSTQ--TIELLDARVLSNHFSQKYPKWDPN 571
            GTFEKLM+ A+++G SI++YKTPR + S Q   I+LL++ ++SN+FSQK PK D N
Sbjct: 541 AGTFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKLDAN 595

[Close]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 8.6e-190
Identity = 327/592 (55.24%), Postives = 420/592 (70.95%), Query Frame = 0


Query: 9   LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 68
           L++K+KQ LQ IE++TSNA ++QRQ L EIL  NADVEYL++H LNG TD  TFKN +PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE---------- 128
           I+YE ++P I+RIANGD SPIL ++PI+EF  S+GT+GGE+KL+P  E+E          
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 ----MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG---GNT 188
               M Q  P    GK M+F F K+ES T  G+ A    TS  K SHF           T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRT 248
           SP +  LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HWI L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGS 308
           GT+   I D SVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I +G+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIER 368
           MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P MAYFEFLP+ R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 EKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKN 428
                           + +QELVDLVDVKLGQEYELV+TTY GL RY +GD+LRV GFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 429 KAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGH 488
           KAP FSF+CRKN++LS+D++KT+E EL N V  A   L PF A++ +YTSY + ++IPGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFE 548
           YVL+WE+ L + ++ I  S++EDCCLA+EE  N  YRQGR  +KSI  LEI+IVE GTF+
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMERAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           KLM+ A+S G SI+QYKTPR +K    IELL++RV+ ++FS K PKW P  +
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHK 607

[Close]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.5e-189
Identity = 325/596 (54.53%), Postives = 423/596 (70.97%), Query Frame = 0


Query: 5   AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 64
           ++  L EK+K  LQ IED+T+NA ++QR+ L EIL+ NADVEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 65  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------ 124
            +PV++YE ++P I+RIANGD S +LC+ PI+EF  S+GT+GGE+KL+P  E+E      
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSL 132

Query: 125 --------MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG-- 184
                   M Q  P   KGK M+F F K+ES T  G+ A    TS  K SHF N      
Sbjct: 133 LYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPY 192

Query: 185 -GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLAN 244
              TSP    LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HW  LA 
Sbjct: 193 TNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELAR 252

Query: 245 DIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAI 304
           DIRTGT+  +I DSSVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I
Sbjct: 253 DIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVI 312

Query: 305 ASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFL 364
            +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P+MAYFEFL
Sbjct: 313 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFL 372

Query: 365 PIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVK 424
           P+ R                + +QELVDLVDVKLGQEYELV+TTY GLYRY +GD+L V 
Sbjct: 373 PVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVA 432

Query: 425 GFKNKAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTT 484
           GFKN AP FSF+CRKN++LS+D++KT+E EL N V  A   L PF A++ +YTSY + ++
Sbjct: 433 GFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 492

Query: 485 IPGHYVLYWEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVEN 544
           IPGHYVL+WE+ L   ++ I  S++EDCCL IEE LN  YRQGR  +KSI  LEI++VE+
Sbjct: 493 IPGHYVLFWELCL-NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 552

Query: 545 GTFEKLMERAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           GTF+KLM+ A+S G SI+QYKTPR +K    IELL++RV+ ++FS K PKW P  +
Sbjct: 553 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHK 607

[Close]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.4e-169
Identity = 293/577 (50.78%), Postives = 392/577 (67.94%), Query Frame = 0


Query: 15  QALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTD-ASTFKNFIPVISYEQ 74
           +AL+ IE +T+NAG++QR+ L EILA NA  EYL+++G+ GS D    F+  +P+++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE--------------M 134
           L+P I RIANGD SPI   +PI+EF  S+GT+GGE+KL+P    E              M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG---GNTSPIDV 194
            Q      KGK M+  F KAES T  G+ A    TS  +   F +         TSP + 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 195 TLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTIDP 254
            LC D+YQS Y+QLLCGL     V ++GA+F S  +  + FLE HW  L +DIRTG +DP
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 255 KIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQYV 314
           +I D  VR++V  +L+ +P LAD IE EC + SWEGII RLWP   YI  I +G+MSQY+
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 315 PLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKANG 374
           P + +Y   LPL    Y SSEC+ GLNL+P+CKP +++YTL+P M Y+EFLP+    A  
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 375 EA-RQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNIIL 434
           EA  ++LVDLVDVKLG EYELV+TTY GLYRY +GD+LRV GFKNKAP FSFV R+N+ L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 435 SLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEESSM 494
           S+D++KT+E ELH  V  A + L+PFGA++V+YTSY +  TIPGHYVL+WE+R    S+ 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELR--AGSTA 501

Query: 495 ISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSISQ 554
           + +S++E+CCL++EE LN  YRQGR  ++SI  LEIR+V  GTF+KLM+ A+S+G SI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 555 YKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           YK PR ++    +ELLDARV   +FS K PKW P ++
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNK 596

[Close]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 4.2e-160
Identity = 273/580 (47.07%), Postives = 389/580 (67.07%), Query Frame = 0


Query: 11  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 70
           E + +AL+ IE+IT N   +Q + L EIL+ N++ EYL++  LNG+ D  +FK+ +PV+ 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------------ 130
           YE LK  I RI+NGD SPIL + PITEF  S+GT+ GE+KL+P  E++            
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 --MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGG-NTSPID 190
             M    P   KGK ++F F K+ESTT  G+ A    TS  K  +F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 191 VTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTID 250
             LC D+ QS Y+Q+LCGL    +V++LGA+F S L+  + FL+N+W  L+ DI TGT+ 
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 251 PKIIDSSVRESVMNIL-KPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQ 310
            KI D +++  + NIL KP+ +LA+F+   C++ +WEGII ++WPN  Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 311 YVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKA 370
           Y+P++ YYS  LP+ S  Y SSE + G+NL+P+CKP E+SYT+ P+MAYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 371 NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNII 430
            G     LV+L DV++G+EYELVITTY GLYRY +GDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 431 LSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEESS 490
           LS++++KT+E +L   V  A+RLL+  G  +++YTSY +  TIPGHYV+YWE+   ++S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 491 MI-SSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSI 550
            + S  +   CCL +EE LN  YRQ R  +KSI  LEIR+V+NGTFE+LM+ ++S+G SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 551 SQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRH 574
           +QYK PR +  T  ++LLD+RV+S HFS   P W P  RH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

[Close]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.7e-159
Identity = 282/580 (48.62%), Postives = 380/580 (65.52%), Query Frame = 0


Query: 11  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 70
           EKD +AL+ IE++T NA  +Q   LAEILA NAD EYL++  L G+TD  TFK  IPVI+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEM--KQLF----- 130
           YE L+P I RIA+GD SPIL   PI+EF  S+GT+ GE+KL+P  ++E+  +QL      
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 -------PNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN---IQSGGNTSP 190
                  P   KGK M+F F K+E+ T  G+ A    TS  K  HF +         TSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 191 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 250
            +  LC D++QS Y+Q+LCGL     V ++GA+F S L+  ++FL+ HW   A+DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 251 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 310
           +D +I D S+R+ +  ILKP+P LA+FI  EC   +WE II R+WPN  Y+  I +G+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 311 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 370
           QY+P + YYS  LP+    Y SSEC+ GLNL+P+ KP E+SYT++P+MAYFEF+P+   K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 371 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNI 430
           A        V+LVDV +G+EYELV+TTY GL RY +GDILRV GF N AP F FV RKN+
Sbjct: 377 A--------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436

Query: 431 ILSLDAEKTEEGELHNGVHEAARLL-SPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEE 490
           +LS+D++KT+E EL   V  A+ +L    G+ + +YTSY + +TIPGHYVLYWE+ + + 
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496

Query: 491 SSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVS 550
           +   S      CCL +EE LN  YRQ R  + S+  LEIR+V NGTFE+LM+ A+S+G S
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556

Query: 551 ISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           I+QYK PR +  T  +ELLD+RV+S HFS   P W P  R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

[Close]

BLAST of Sed0007014 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 7.7e-234
Identity = 394/580 (67.93%), Postives = 462/580 (79.66%), Query Frame = 0


Query: 9   LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 68
           L+EKDKQALQ +ED+T  A EIQR+ L EIL+TNA VEYLQQHGLNGSTDASTFKN IP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE---------- 128
           +SYE LKPYIDRI NGD+SPILC  PITEF+ STGT+GGEQKLIP  E+E          
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 ----MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGGNTSP 188
               MKQLFP  H GKEMFF F K ES TKAGI+A S STS  K  H NN++     TSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 248
            D+  CTD YQS Y QLLCGL QN+KV ++G+LF STLIY+ KFLENHW+LLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 308
           I+ KI+DS VR+SVMNILKP+PQLADFIE EC+KG+WEGI+ RLWPN  +I A+ +GSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 368
            Y+P +NYY  +LPLI+  Y SSECF G+NLDPLCKPEEISYT +P +AYFEFLP++R  
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNI 428
            +GE  QE VDLVDVKLGQEYE+V+TTY GLYRYC+GDI+RV GFKNKAPNF FV RKN+
Sbjct: 364 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 423

Query: 429 ILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEES 488
           ILSLD EKT+E ELHN V++   LLS FGA +VDYTSY +++T PGHYVLYWEI+ +EE 
Sbjct: 424 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEI 483

Query: 489 SMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSI 548
           S+I SSIYEDCCLAIEE L+  YR+ R  +K IN LEI+IVE  TF+KLMER V QG SI
Sbjct: 484 SIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 543

Query: 549 SQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRH 574
           SQYKTPR +KSTQ IE LD++V++N+FS+KYPKWDP   H
Sbjct: 544 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Sed0007014 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.4e-227
Identity = 398/593 (67.12%), Postives = 475/593 (80.10%), Query Frame = 0


Query: 1   MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 60
           M+H  E   +E+DKQALQHIED+T +A +IQRQ L +IL+TNADVEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE-- 120
           TFKN IP+ISYEQL+PYIDRIANGD+SPILCT PITEFFIS+GT+GGE KLIP++E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 ------------MKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN 180
                       MKQLFPNS   KGK M F F K E  T++GI+A++ +TSLCKR++ N 
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN- 180

Query: 181 IQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWIL 240
                NTSP D+ LCTDT QS Y QLLCGL QN+ V ++GALF STLI+VLKFLE+HW++
Sbjct: 181 -----NTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYI 300
           LA+DIR GT++ KI D SVRE+VMNIL PN QLADFIE EC+KG+WEGII RLWPNA YI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 KAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYF 360
            AI +GSM+QY+P +NYYSN+LP++SDHYG+SECFLGLNLDPLC P+  SYTL+P MAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPN 420
           EFLPI+R K++ E  +ELVDLVDVKLG EYELVITTY GLYRY MGDIL V GFKNKAPN
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLY 480
           FSFVCRKN++LSLD EKT+E ELH+ V EAARLLSPFGAAI DYTS+ +++ IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 WEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLME 540
           WEI ++   S I SSIYEDCC AIEELLN  YR  R  EK IN LEI+IV+ GTFEKLM 
Sbjct: 481 WEIHVKGGIS-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMA 540

Query: 541 RAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRHHLFS 578
           +A+++G S+SQYKTPR + ST  IELLD+ V+  +FS+K PK D  + H L S
Sbjct: 541 QALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Sed0007014 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 4.4e-221
Identity = 381/578 (65.92%), Postives = 457/578 (79.07%), Query Frame = 0


Query: 5   AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 64
           ++++L+ KDKQ L HIED TSNA EIQR+ + EIL+ NA VEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------ 124
            IPV+SYEQLKPYIDRIA+GD+SPILC + IT+F ISTGT+GG QKLIP+F+ E      
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 --------MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQ-SGG 184
                   MKQLFP    GKEMFF FT+AE+ TK G++A   S+ L K S F++++    
Sbjct: 122 TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRA 181

Query: 185 NTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDI 244
            TSPID+  CTD YQS Y QLLCGL QN+ V ++GALF STLIY+ KFLENHW LL NDI
Sbjct: 182 YTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDI 241

Query: 245 RTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIAS 304
           RTGT+D KI D  VRESVM++LKPNP+LADFIE EC+KG+WEGI+ RLWPN  YI  + +
Sbjct: 242 RTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVT 301

Query: 305 GSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPI 364
           GSMSQY+P +N+Y    P++S  Y SSECF G+NLDPLCKPEEISYT +P MAYFEFLP+
Sbjct: 302 GSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPV 361

Query: 365 EREKA-NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFV 424
           +R  + NGE  QELVDLVDVKLGQEYE+V+TTY GLYRYCMGDILRV GFKNK PNFSF 
Sbjct: 362 DRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFA 421

Query: 425 CRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIR 484
           CRKN+ILSLD EKT+E +LH+ V EA+ LLSP GA IVDYTSY +++T PGHYVLYWEI+
Sbjct: 422 CRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQ 481

Query: 485 LEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVS 544
             EE+S I S++YEDCCLA+EE L+  YRQ R   K IN LEI+IVE GTFEKLME+AV 
Sbjct: 482 CREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVD 541

Query: 545 QGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPK 567
           +G SISQYKTPR +KSTQ IELLD++V+SN+FSQ+YPK
Sbjct: 542 RGASISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

[Close]

BLAST of Sed0007014 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 5.0e-217
Identity = 371/569 (65.20%), Postives = 438/569 (76.98%), Query Frame = 0


Query: 9   LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 68
           L+EKDKQ LQ++ED+TSNA EIQR+ LAEIL+TNA VEYL+QHGL+GSTD STFKN IP+
Sbjct: 4   LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63

Query: 69  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEMKQLFPNSHK 128
           +SYE LKPYIDRI++GD+SPILC  PIT+                               
Sbjct: 64  VSYEHLKPYIDRISSGDDSPILCAHPITD------------------------------- 123

Query: 129 GKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGGNTSPIDVTLCTDTYQSTYS 188
                  F +AES TK GI+A S STSL + S FNN++SG  TSP ++  CTD YQS Y 
Sbjct: 124 -------FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTSPNEIIRCTDAYQSLYC 183

Query: 189 QLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTIDPKIIDSSVRESVM 248
           QLLCGL Q EKV  +GALF STL+YV KFLENHW+LLANDIRTGT++ KI D  VRESVM
Sbjct: 184 QLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTVNTKITDQLVRESVM 243

Query: 249 NILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQYVPLINYYSNNLPL 308
           NILKP+PQLADFIE EC+KG+WEGI+ RLWPN  +I AI +GSMSQY+P +NYYSN LPL
Sbjct: 244 NILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMSQYIPQLNYYSNCLPL 303

Query: 309 ISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKANGEARQELVDLVDV 368
           IS  Y SSEC+ G+NLDPL KPEEISYT +P MAYFEFLP++R  AN E  QELVDLVDV
Sbjct: 304 ISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRANANREVGQELVDLVDV 363

Query: 369 KLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNIILSLDAEKTEEGELH 428
           KLGQEYE+++TTY GLYRYC+GDI+RV GFK KAP FSFVCRKN++LSLD EKT+E ELH
Sbjct: 364 KLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNVVLSLDFEKTDEAELH 423

Query: 429 NGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEESSMISSSIYEDCCLAI 488
           N V  A  LL+PFGA I DYTSY +N+T PGHYVLYWEI  +EE S+I SSIYEDCCLA+
Sbjct: 424 NAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEISIIPSSIYEDCCLAV 483

Query: 489 EELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSISQYKTPRSIKSTQTI 548
           EE LN  YRQ R  +K IN LEI++VE GTF+KL+ERA  QG SISQYKTPR + STQ +
Sbjct: 484 EESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASISQYKTPRCVTSTQIV 534

Query: 549 ELLDARVLSNHFSQKYPKWDPNSRHHLFS 578
           ELLD +V++++FS+KYPKWDP  RH LF+
Sbjct: 544 ELLDTKVVASYFSKKYPKWDPTCRHRLFA 534

[Close]

BLAST of Sed0007014 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 7.8e-210
Identity = 369/589 (62.65%), Postives = 453/589 (76.91%), Query Frame = 0


Query: 1   MSHEAENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDAS 60
           MS+  E  L+EKDK+ALQ+IEDIT+ A EIQRQ L EIL+ NA+VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNFIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE-- 120
           TFK  IP++ Y+QL+PYI RIA+GD+S ILC+ PITEFF S+GT+G E KLIP++E+E  
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 ------------MKQLFPNS--HKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKR-SHFN 180
                       MKQLFPN   HK K + F F K E  TK GI+  S  T+L KR S+  
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 NIQSGGNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWI 240
           ++ SG NTSP D+ LCTD+YQS Y QLLCGLYQN+ V ++ ALF STLI+V KFLENHWI
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 LLANDIRTGTIDPKIIDSSVRESVMNI-LKPNPQLADFIEIECNKGSWEGIIPRLWPNAN 300
            LA DIRT TI+PKI  SSVRESVM I +KPNP++AD IE EC KG WEGII RLWPNA 
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YIKAIASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMA 360
           YI A+ +GSMSQY+PL+NYY+NNLP++SDHYGSSECFLGLNLDPLC P+E+SYTL+P MA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

Query: 361 YFEFLPIEREKANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKA 420
           YFEFLPI+     GE  Q LVDLVDVKLG EYELVITT+ GLYRYCMGD++RV GFKNKA
Sbjct: 361 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 420

Query: 421 PNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYV 480
           PNFSFV RKN++L++  EKT+E  LH  V E  R++  FGA IVDYT+Y + +TIPGHYV
Sbjct: 421 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 480

Query: 481 LYWEIRL----EEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGT 540
           LYWE+ L    E+  + I  S++EDCCLAIE  LN+ YR  R  EK IN LEI+IV+ GT
Sbjct: 481 LYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGT 540

Query: 541 FEKLMERAVSQGVSISQYKTPRSIKSTQ--TIELLDARVLSNHFSQKYP 566
           FEKLM+ A+++G SI+QYKTPR + S+Q   I+LL++ ++SN+FS+ YP
Sbjct: 541 FEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

[Close]

BLAST of Sed0007014 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 664.8 bits (1714), Expect = 6.1e-191
Identity = 327/592 (55.24%), Postives = 420/592 (70.95%), Query Frame = 0


Query: 9   LEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPV 68
           L++K+KQ LQ IE++TSNA ++QRQ L EIL  NADVEYL++H LNG TD  TFKN +PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE---------- 128
           I+YE ++P I+RIANGD SPIL ++PI+EF  S+GT+GGE+KL+P  E+E          
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 ----MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG---GNT 188
               M Q  P    GK M+F F K+ES T  G+ A    TS  K SHF           T
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRT 248
           SP +  LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HWI L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGS 308
           GT+   I D SVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I +G+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIER 368
           MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P MAYFEFLP+ R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 EKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKN 428
                           + +QELVDLVDVKLGQEYELV+TTY GL RY +GD+LRV GFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 429 KAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGH 488
           KAP FSF+CRKN++LS+D++KT+E EL N V  A   L PF A++ +YTSY + ++IPGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 489 YVLYWEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFE 548
           YVL+WE+ L + ++ I  S++EDCCLA+EE  N  YRQGR  +KSI  LEI+IVE GTF+
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMERAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           KLM+ A+S G SI+QYKTPR +K    IELL++RV+ ++FS K PKW P  +
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHK 607

[Close]

BLAST of Sed0007014 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 663.3 bits (1710), Expect = 1.8e-190
Identity = 325/596 (54.53%), Postives = 423/596 (70.97%), Query Frame = 0


Query: 5   AENLLEEKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKN 64
           ++  L EK+K  LQ IED+T+NA ++QR+ L EIL+ NADVEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 65  FIPVISYEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------ 124
            +PV++YE ++P I+RIANGD S +LC+ PI+EF  S+GT+GGE+KL+P  E+E      
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSL 132

Query: 125 --------MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSG-- 184
                   M Q  P   KGK M+F F K+ES T  G+ A    TS  K SHF N      
Sbjct: 133 LYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPY 192

Query: 185 -GNTSPIDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLAN 244
              TSP    LC+D+YQS YSQ+LCGL Q+++V ++GA+F S  I  +KFLE HW  LA 
Sbjct: 193 TNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELAR 252

Query: 245 DIRTGTIDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAI 304
           DIRTGT+  +I DSSVRE+V  ILKP+P+LADF+E EC K SW+GII RLWPN  Y+  I
Sbjct: 253 DIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVI 312

Query: 305 ASGSMSQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFL 364
            +G+MSQY+P ++YYSN LPL+   Y SSEC+ G+NL PLCKP E+SYTL+P+MAYFEFL
Sbjct: 313 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFL 372

Query: 365 PIEREKA-----------NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVK 424
           P+ R                + +QELVDLVDVKLGQEYELV+TTY GLYRY +GD+L V 
Sbjct: 373 PVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVA 432

Query: 425 GFKNKAPNFSFVCRKNIILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTT 484
           GFKN AP FSF+CRKN++LS+D++KT+E EL N V  A   L PF A++ +YTSY + ++
Sbjct: 433 GFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 492

Query: 485 IPGHYVLYWEIRLEEESSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVEN 544
           IPGHYVL+WE+ L   ++ I  S++EDCCL IEE LN  YRQGR  +KSI  LEI++VE+
Sbjct: 493 IPGHYVLFWELCL-NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 552

Query: 545 GTFEKLMERAVSQGVSISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           GTF+KLM+ A+S G SI+QYKTPR +K    IELL++RV+ ++FS K PKW P  +
Sbjct: 553 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHK 607

[Close]

BLAST of Sed0007014 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 566.2 bits (1458), Expect = 3.0e-161
Identity = 273/580 (47.07%), Postives = 389/580 (67.07%), Query Frame = 0


Query: 11  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 70
           E + +AL+ IE+IT N   +Q + L EIL+ N++ EYL++  LNG+ D  +FK+ +PV+ 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKE------------ 130
           YE LK  I RI+NGD SPIL + PITEF  S+GT+ GE+KL+P  E++            
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 --MKQLFPNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNNIQSGG-NTSPID 190
             M    P   KGK ++F F K+ESTT  G+ A    TS  K  +F    S    TSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 191 VTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTID 250
             LC D+ QS Y+Q+LCGL    +V++LGA+F S L+  + FL+N+W  L+ DI TGT+ 
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 251 PKIIDSSVRESVMNIL-KPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMSQ 310
            KI D +++  + NIL KP+ +LA+F+   C++ +WEGII ++WPN  Y+  I +G+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 311 YVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREKA 370
           Y+P++ YYS  LP+ S  Y SSE + G+NL+P+CKP E+SYT+ P+MAYFEFLP   +  
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 371 NGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNII 430
            G     LV+L DV++G+EYELVITTY GLYRY +GDILRV GF N AP F F+ R+N++
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 431 LSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEESS 490
           LS++++KT+E +L   V  A+RLL+  G  +++YTSY +  TIPGHYV+YWE+   ++S+
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 491 MI-SSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVSI 550
            + S  +   CCL +EE LN  YRQ R  +KSI  LEIR+V+NGTFE+LM+ ++S+G SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 551 SQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSRH 574
           +QYK PR +  T  ++LLD+RV+S HFS   P W P  RH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

[Close]

BLAST of Sed0007014 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 563.5 bits (1451), Expect = 1.9e-160
Identity = 282/580 (48.62%), Postives = 380/580 (65.52%), Query Frame = 0


Query: 11  EKDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVIS 70
           EKD +AL+ IE++T NA  +Q   LAEILA NAD EYL++  L G+TD  TFK  IPVI+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEM--KQLF----- 130
           YE L+P I RIA+GD SPIL   PI+EF  S+GT+ GE+KL+P  ++E+  +QL      
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 -------PNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFNN---IQSGGNTSP 190
                  P   KGK M+F F K+E+ T  G+ A    TS  K  HF +         TSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 191 IDVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGT 250
            +  LC D++QS Y+Q+LCGL     V ++GA+F S L+  ++FL+ HW   A+DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 251 IDPKIIDSSVRESVMNILKPNPQLADFIEIECNKGSWEGIIPRLWPNANYIKAIASGSMS 310
           +D +I D S+R+ +  ILKP+P LA+FI  EC   +WE II R+WPN  Y+  I +G+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 311 QYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIEREK 370
           QY+P + YYS  LP+    Y SSEC+ GLNL+P+ KP E+SYT++P+MAYFEF+P+   K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 371 ANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKNI 430
           A        V+LVDV +G+EYELV+TTY GL RY +GDILRV GF N AP F FV RKN+
Sbjct: 377 A--------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436

Query: 431 ILSLDAEKTEEGELHNGVHEAARLL-SPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEE 490
           +LS+D++KT+E EL   V  A+ +L    G+ + +YTSY + +TIPGHYVLYWE+ + + 
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496

Query: 491 SSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVS 550
           +   S      CCL +EE LN  YRQ R  + S+  LEIR+V NGTFE+LM+ A+S+G S
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556

Query: 551 ISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           I+QYK PR +  T  +ELLD+RV+S HFS   P W P  R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

[Close]

BLAST of Sed0007014 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.2e-158
Identity = 277/580 (47.76%), Postives = 384/580 (66.21%), Query Frame = 0


Query: 12  KDKQALQHIEDITSNAGEIQRQTLAEILATNADVEYLQQHGLNGSTDASTFKNFIPVISY 71
           KD +AL+ IE++T N   +Q++ + EIL+ N+D EYL++ GL G TD  TFK  +PV+ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIDRIANGDNSPILCTEPITEFFISTGTTGGEQKLIPIFEKEM--KQLF------ 131
           + LKP I RIANGD S IL + PITEF  S+GT+ GE+KL+P  +++M  +QL       
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 ------PNSHKGKEMFFCFTKAESTTKAGIIAYSTSTSLCKRSHFN---NIQSGGNTSPI 191
                 P   KGK ++F F K ES T  G+ A    TS  K   F    N      TSP 
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 192 DVTLCTDTYQSTYSQLLCGLYQNEKVHKLGALFVSTLIYVLKFLENHWILLANDIRTGTI 251
           +  LC D+ QS Y+Q+LCGL    +V +LGA+F S L+  + FL+ +W  LA+DI TGT+
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 252 DPKIIDSSVRESVMNIL-KPNPQLADFIEIECNK-GSWEGIIPRLWPNANYIKAIASGSM 311
             +I D +++ES+  IL KP+ +LADFI   C +  SWEGII ++WPN  Y+  I +G+M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 312 SQYVPLINYYSNNLPLISDHYGSSECFLGLNLDPLCKPEEISYTLVPDMAYFEFLPIERE 371
           +QY+P++ YYS  LP+    Y SSE + G+NL P+CKP E+SYT++P+MAYFEFLP    
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 372 KANGEARQELVDLVDVKLGQEYELVITTYGGLYRYCMGDILRVKGFKNKAPNFSFVCRKN 431
                 + ELV+L DV++G+EYELVITTY GL RY +GDIL+V GF N AP F FV RKN
Sbjct: 378 PTE---KSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRKN 437

Query: 432 IILSLDAEKTEEGELHNGVHEAARLLSPFGAAIVDYTSYGNNTTIPGHYVLYWEIRLEEE 491
           ++LS++++KT+E EL + V  A+ LL   G  +++YTSY    TIPGHYV+YWE+ ++++
Sbjct: 438 VLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQ 497

Query: 492 SSMISSSIYEDCCLAIEELLNVRYRQGRCQEKSINALEIRIVENGTFEKLMERAVSQGVS 551
           ++  +  +   CCL +EE LN  YRQ R  +KSI  LEIR+V+NGTFE+LM+ A+S+G S
Sbjct: 498 TNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGAS 557

Query: 552 ISQYKTPRSIKSTQTIELLDARVLSNHFSQKYPKWDPNSR 573
           I+QYK PR +  T  +ELLD+RV+S HFS   P W P  R
Sbjct: 558 INQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

The following BLAST results are available for this feature:

BLAST of Sed0007014 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140778.1	1.6e-233	67.93	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
KAG6570863.1	1.1e-229	67.45	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	5.0e-227	67.12	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_022140368.1	9.1e-221	65.92	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]
XP_038902067.1	2.5e-218	65.33	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]

BLAST of Sed0007014 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	8.6e-190	55.24	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	2.5e-189	54.53	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	8.4e-169	50.78	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q9LQ68	4.2e-160	47.07	Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O82333	2.7e-159	48.62	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Sed0007014 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIS9	7.7e-234	67.93	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1FUU5	2.4e-227	67.12	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CHU9	4.4e-221	65.92	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CG61	5.0e-217	65.20	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A5A7V3L5	7.8e-210	62.65	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]

BLAST of Sed0007014 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	6.1e-191	55.24	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.8e-190	54.53	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	3.0e-161	47.07	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	1.9e-160	48.62	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	5.2e-158	47.76	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 20..545 e-value: 1.7E-177 score: 591.5
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 9..572
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 9..572

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0007014.1	Sed0007014.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity