Sed0006761 (gene) Chayote v1

Overview
NameSed0006761
Typegene
OrganismSechium edule (Chayote v1)
Descriptioncrooked neck-like protein 1
LocationLG10: 6025721 .. 6027310 (+)
RNA-Seq ExpressionSed0006761
SyntenySed0006761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCGTTCAAAGATTCTGATCAAACCCTTGGCTACCTGACTCGAAACGATCTGAATGTGAAGCTGCCGCGACCCATTAGGGTGAAGAACAAGAATCCAGCGCCAATTCAGATTACTGCAGAGCAAATCCTTCGTGAAGCTCGAGAGCGACACGAGGCGGAGATTAGACCACCCAAGCGGAAAATTACTGATTCAACTGAACTTGCTGATTATATTTTGTGCAAGCGAAAGGAATTCGAGGATCTTGTCCGCCGTGTTAGGTGGAACATTGGTGTTTGGATTAAGTATGCGCGATGGGAAGAGTCTCAGAAGGAGTTCGATCGAGCTCGTTCGGTTTGGGAGCGTGTGCTCGAGGTCGATAATCGAAATCACACCCTTTGGATCAAGTATGCAGAGGTTGAGATGAAGAATGAGTTTATCAATCATGCGAGGAATGTTTGGGATCGAGCGGCCGCCCTCTTGCCTAGAGTGGATCAGTTGTGGTACATATGGAGGAAATGTTGGGTAATGTGGGTGGTGCAAGGCAGGTTTTTGAGAGGTGGATGGGTTGGATGCCTGACCAGGAAGGTTGGCTCTCTTACATCAAGTTCGAGTTGAGATATGATGAGGTTGAACGTGCTCGAGGCGTATTTGAGCGCTTTGTTCTGTGTCACAACAAAGTTGGGGCATGGATAGGTTTTGCAAAATTTGAGATGAAGAATGGGGAGGTTGCAAAAGCAAGGAATGTTTACGAGAGAGCAGTTGAGAAGTTGGTTGATGATGAAGAGGCCGAAGAACTGTTGGTTGCCTTTGCTGAATTCGAGGAGAGTTGTAAGGAAATTGAACGTGCACGGTGTATATATAAGTTTTCCTTGGATCATATTCCCAAAGGTAGGGCGAAGGATATGTATAGGAAGTTTGTAGCATTTGAGATGCAATATGGGAATAAGGCAGAAACAGAAGATGCTATTGTGGGGGAAGAGGAGGTTTCAATACGAGGAACAAGTGAAGAAGAATCCTCTAGATTATGATTCATGGTTTGAATATATACAATTGGAGGAAACTACGGGGAACACGGAGAGGATTAGAGAAGTGTACGAAAGAGCAATAGCCAATGTTCCTCCAGCCGAAGAGAAACGTTATTGGAGGCGTTACATATATTTATGGATAAACTATGCCGTGTACGAAGAATTGAATGCGGGAGACATTGAACGTACTCGTGATGTTTATAGGCAATGTCTAAGCTTGATTCCGCATAAGAAGTTCTCCTTTGCAAAGATTTGGCTGCTAGCTGCCCAATTTGAATTAAGACAATTGAACCTTAAAGCTGCTCGACAAATATTAGGCAATGCAATTGGGAGGGCTCCTAAAGAGAAGATTTTCGAGAAGTATATCGAGATAGAACTGCAGTTGGAAAATATAGATCGGTGTCGAAAATTGTATGAGAAGTACCTTCTGTGGTCGCCTAAAGATTGCTATGCATGGAACAAGTATGGTTTGGCTGGGTTCGAACACACTGATTACTTGCCCCCCAATGAAACACAGCCTACAAATTTGAAGATCCTTGAAGCTGCATACCGTTGGAAGAAGCAAAAAGTTGATCATTGA

mRNA sequence

ATGTCTTCGTTCAAAGATTCTGATCAAACCCTTGGCTACCTGACTCGAAACGATCTGAATGTGAAGCTGCCGCGACCCATTAGGGTGAAGAACAAGAATCCAGCGCCAATTCAGATTACTGCAGAGCAAATCCTTCGTGAAGCTCGAGAGCGACACGAGGCGGAGATTAGACCACCCAAGCGGAAAATTACTGATTCAACTGAACTTGCTGATTATATTTTGTGCAAGCGAAAGGAATTCGAGGATCTTGTCCGCCGTGTTAGGTGGAACATTGGTGTTTGGATTAAGTATGCGCGATGGGAAGAGTCTCAGAAGGAGTTCGATCGAGCTCGTTCGGTTTGGGAGCGTGTGCTCGAGGTCGATAATCGAAATCACACCCTTTGGATCAAGTATGCAGAGGTTGAGATGAAGAATGAGTTTATCAATCATGCGAGGAATGTTTGGGATCGAGCGGCCGCCCTCTTGCCTAGAGTGGATCAGTTGTGGCAGGTTTTTGAGAGGTGGATGGGTTGGATGCCTGACCAGGAAGGTTGGCTCTCTTACATCAAGTTCGAGTTGAGATATGATGAGGTTGAACGTGCTCGAGGCGTATTTGAGCGCTTTGTTCTGTGTCACAACAAAGTTGGGGCATGGATAGGTTTTGCAAAATTTGAGATGAAGAATGGGGAGGTTGCAAAAGCAAGGAATGTTTACGAGAGAGCAGTTGAGAAGTTGGTTGATGATGAAGAGGCCGAAGAACTGTTGGTTGCCTTTGCTGAATTCGAGGAGAGTTGTAAGGAAATTGAACGTGCACGGTGTATATATAAGTTTTCCTTGGATCATATTCCCAAAGGTAGGGCGAAGGATATGTATAGGAAGTTTGTAGCATTTGAGATGCAATATGGGAATAAGGCAGAAACAGAAGATGCTATTAAGAATCCTCTAGATTATGATTCATGGTTTGAATATATACAATTGGAGGAAACTACGGGGAACACGGAGAGGATTAGAGAAGTGTACGAAAGAGCAATAGCCAATGTTCCTCCAGCCGAAGAGAAACGTTATTGGAGGCGTTACATATATTTATGGATAAACTATGCCGTGTACGAAGAATTGAATGCGGGAGACATTGAACGTACTCGTGATGTTTATAGGCAATGTCTAAGCTTGATTCCGCATAAGAAGTTCTCCTTTGCAAAGATTTGGCTGCTAGCTGCCCAATTTGAATTAAGACAATTGAACCTTAAAGCTGCTCGACAAATATTAGGCAATGCAATTGGGAGGGCTCCTAAAGAGAAGATTTTCGAGAAGTATATCGAGATAGAACTGCAGTTGGAAAATATAGATCGGTGTCGAAAATTGTATGAGAAGTACCTTCTGTGGTCGCCTAAAGATTGCTATGCATGGAACAAGTATGGTTTGGCTGGGTTCGAACACACTGATTACTTGCCCCCCAATGAAACACAGCCTACAAATTTGAAGATCCTTGAAGCTGCATACCGTTGGAAGAAGCAAAAAGTTGATCATTGA

Coding sequence (CDS)

ATGTCTTCGTTCAAAGATTCTGATCAAACCCTTGGCTACCTGACTCGAAACGATCTGAATGTGAAGCTGCCGCGACCCATTAGGGTGAAGAACAAGAATCCAGCGCCAATTCAGATTACTGCAGAGCAAATCCTTCGTGAAGCTCGAGAGCGACACGAGGCGGAGATTAGACCACCCAAGCGGAAAATTACTGATTCAACTGAACTTGCTGATTATATTTTGTGCAAGCGAAAGGAATTCGAGGATCTTGTCCGCCGTGTTAGGTGGAACATTGGTGTTTGGATTAAGTATGCGCGATGGGAAGAGTCTCAGAAGGAGTTCGATCGAGCTCGTTCGGTTTGGGAGCGTGTGCTCGAGGTCGATAATCGAAATCACACCCTTTGGATCAAGTATGCAGAGGTTGAGATGAAGAATGAGTTTATCAATCATGCGAGGAATGTTTGGGATCGAGCGGCCGCCCTCTTGCCTAGAGTGGATCAGTTGTGGCAGGTTTTTGAGAGGTGGATGGGTTGGATGCCTGACCAGGAAGGTTGGCTCTCTTACATCAAGTTCGAGTTGAGATATGATGAGGTTGAACGTGCTCGAGGCGTATTTGAGCGCTTTGTTCTGTGTCACAACAAAGTTGGGGCATGGATAGGTTTTGCAAAATTTGAGATGAAGAATGGGGAGGTTGCAAAAGCAAGGAATGTTTACGAGAGAGCAGTTGAGAAGTTGGTTGATGATGAAGAGGCCGAAGAACTGTTGGTTGCCTTTGCTGAATTCGAGGAGAGTTGTAAGGAAATTGAACGTGCACGGTGTATATATAAGTTTTCCTTGGATCATATTCCCAAAGGTAGGGCGAAGGATATGTATAGGAAGTTTGTAGCATTTGAGATGCAATATGGGAATAAGGCAGAAACAGAAGATGCTATTAAGAATCCTCTAGATTATGATTCATGGTTTGAATATATACAATTGGAGGAAACTACGGGGAACACGGAGAGGATTAGAGAAGTGTACGAAAGAGCAATAGCCAATGTTCCTCCAGCCGAAGAGAAACGTTATTGGAGGCGTTACATATATTTATGGATAAACTATGCCGTGTACGAAGAATTGAATGCGGGAGACATTGAACGTACTCGTGATGTTTATAGGCAATGTCTAAGCTTGATTCCGCATAAGAAGTTCTCCTTTGCAAAGATTTGGCTGCTAGCTGCCCAATTTGAATTAAGACAATTGAACCTTAAAGCTGCTCGACAAATATTAGGCAATGCAATTGGGAGGGCTCCTAAAGAGAAGATTTTCGAGAAGTATATCGAGATAGAACTGCAGTTGGAAAATATAGATCGGTGTCGAAAATTGTATGAGAAGTACCTTCTGTGGTCGCCTAAAGATTGCTATGCATGGAACAAGTATGGTTTGGCTGGGTTCGAACACACTGATTACTTGCCCCCCAATGAAACACAGCCTACAAATTTGAAGATCCTTGAAGCTGCATACCGTTGGAAGAAGCAAAAAGTTGATCATTGA

Protein sequence

MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPKRKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDAIKNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKYGLAGFEHTDYLPPNETQPTNLKILEAAYRWKKQKVDH
Homology
BLAST of Sed0006761 vs. NCBI nr
Match: XP_022152908.1 (crooked neck-like protein 1 [Momordica charantia] >XP_022152909.1 crooked neck-like protein 1 [Momordica charantia] >XP_022152910.1 crooked neck-like protein 1 [Momordica charantia])

HSP 1 Score: 812.8 bits (2098), Expect = 1.7e-231
Identity = 398/495 (80.40%), Postives = 431/495 (87.07%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWNI VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNISVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           +FERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 IFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           VAKARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 VAKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDI 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAIANVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAIANVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHGKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL+ ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLQGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. NCBI nr
Match: XP_022152908.1 (crooked neck-like protein 1 [Momordica charantia] >XP_022152909.1 crooked neck-like protein 1 [Momordica charantia] >XP_022152910.1 crooked neck-like protein 1 [Momordica charantia])

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-17
Identity = 112/418 (26.79%), Postives = 182/418 (43.54%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA A +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAIANVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHGKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L+  P+     
Sbjct: 444 NLQGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLEWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKF-SFAK 408
           R +             +++ +WI+YA +E     D  R        LS +P      + +
Sbjct: 564 RLLDRT----------KHLKVWISYAKFEASAMEDDSR--------LSELPEDNVQEYLQ 623

Query: 409 IWLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRC 468
            W        ++  ++ AR++   AI      AP+ K     + E+++ +E    ++   
Sbjct: 624 SW--------KKQCIQHARRVFEKAITYYRNSAPELKEERAMLLEEWLNMEASFGDLGDV 683

Query: 469 RKLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
             +  K      K      + G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 SLVQSKLPKKLKKRRQIATEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 812.8 bits (2098), Expect = 1.7e-231
Identity = 396/495 (80.00%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKFSLDHIPKGRA+D+
Sbjct: 241 IGKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFSLDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. NCBI nr
Match: XP_023519624.1 (crooked neck-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 100.1 bits (248), Expect = 5.6e-17
Identity = 114/417 (27.34%), Postives = 183/417 (43.88%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA + +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAISNVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L   P+     
Sbjct: 444 NLNGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLVWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKI 408
           R +             +++ +WI+YA YE     D  +        LS +P  K    + 
Sbjct: 564 RLLDRT----------KHLKVWISYAKYEASAMEDDSQ--------LSELPEDK---VQE 623

Query: 409 WLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRCR 468
           +L A     +Q  ++ AR++   AI      AP+ K     + E+++ +E     +    
Sbjct: 624 YLRAR----KQQCIQQARRVFEKAITYYRNSAPELKEERAMLLEEWLNMETSFGELGDVS 683

Query: 469 KLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
            +  K      K     ++ G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 LVQSKLPKKLKKRRQIASEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 812.8 bits (2098), Expect = 1.7e-231
Identity = 396/495 (80.00%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKFSLDHIPKGRA+D+
Sbjct: 241 IGKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFSLDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. NCBI nr
Match: XP_022970297.1 (crooked neck-like protein 1 [Cucurbita maxima] >XP_022970298.1 crooked neck-like protein 1 [Cucurbita maxima] >XP_022970299.1 crooked neck-like protein 1 [Cucurbita maxima] >XP_022970301.1 crooked neck-like protein 1 [Cucurbita maxima] >XP_022970302.1 crooked neck-like protein 1 [Cucurbita maxima])

HSP 1 Score: 99.4 bits (246), Expect = 9.5e-17
Identity = 109/417 (26.14%), Postives = 179/417 (42.93%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA + +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAISNVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L   P+     
Sbjct: 444 NLNGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLVWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKI 408
           R +             +++ +WI+YA YE     D  +  ++    +    H +      
Sbjct: 564 RLLDRT----------KHLKVWISYAKYEASAMEDDSQLPELPEDKVQEYLHAR------ 623

Query: 409 WLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRCR 468
                    +Q  ++ AR++   AI      AP+ K     + E+++ +E     +    
Sbjct: 624 ---------KQQCIQQARRVFEKAITYYRNSAPELKEERAMLLEEWLNMETSFGELGDVS 683

Query: 469 KLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
            +  K      K     ++ G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 LVQSKLPKKLKKRRQIASEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 811.6 bits (2095), Expect = 3.8e-231
Identity = 395/495 (79.80%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 ITKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. NCBI nr
Match: XP_022964730.1 (crooked neck-like protein 1 [Cucurbita moschata] >XP_022964731.1 crooked neck-like protein 1 [Cucurbita moschata])

HSP 1 Score: 100.1 bits (248), Expect = 5.6e-17
Identity = 114/417 (27.34%), Postives = 183/417 (43.88%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA + +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAISNVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L   P+     
Sbjct: 444 NLNGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLVWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKI 408
           R +             +++ +WI+YA YE     D  +        LS +P  K    + 
Sbjct: 564 RLLDRT----------KHLKVWISYAKYEASAMEDDSQ--------LSELPEDK---VQE 623

Query: 409 WLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRCR 468
           +L A     +Q  ++ AR++   AI      AP+ K     + E+++ +E     +    
Sbjct: 624 YLRAR----KQQCIQQARRVFEKAITYYRNSAPELKEERAMLLEEWLNMETSFGELGDVS 683

Query: 469 KLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
            +  K      K     ++ G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 LVQSKLPKKLKKRRQIASEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 811.6 bits (2095), Expect = 3.8e-231
Identity = 395/495 (79.80%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 ITKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. ExPASy Swiss-Prot
Match: Q9BZJ0 (Crooked neck-like protein 1 OS=Homo sapiens OX=9606 GN=CRNKL1 PE=1 SV=4)

HSP 1 Score: 526.9 bits (1356), Expect = 2.4e-148
Identity = 264/475 (55.58%), Postives = 336/475 (70.74%), Query Frame = 0

Query: 22  KLPRPIRVKNKNPAPIQITAEQILREARERH-EAEIRPPKRKITDSTELADYILCKRKEF 81
           ++P+  +VKNK PA +QITAEQ+LREA+ER  E    PP++KITD  EL DY L KRK F
Sbjct: 172 RIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTF 231

Query: 82  EDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEF 141
           ED +R+ R  I  WIKYA+WEES KE  RARS++ER L+VD RN TLW+KYAE+EMKN  
Sbjct: 232 EDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQ 291

Query: 142 INHARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIK 201
           +NHARN+WDRA   LPRV+Q W                 QVFERWM W P+++ W SYI 
Sbjct: 292 VNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYIN 351

Query: 202 FELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEE 261
           FELRY EV+RAR ++ERFVL H  V  WI +A+FE K+   A AR VYERAVE   D+  
Sbjct: 352 FELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHM 411

Query: 262 AEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDA 321
            E L VAFA+FEE+ KE ER R IYK++LD I K  A+++++ +  FE ++G++   ED 
Sbjct: 412 DEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDI 471

Query: 322 I-------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWR 381
           I              NP +YD+WF+Y++L E+    E +REVYERAIANVPP +EKR+W+
Sbjct: 472 IVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWK 531

Query: 382 RYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKA 441
           RYIYLWINYA+YEEL A D ERTR VY+  L LIPHKKF+FAK+W+L AQFE+RQ NL  
Sbjct: 532 RYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSL 591

Query: 442 ARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKY 466
           AR+ LG +IG+ PK K+F+ YIE+ELQL   DRCRKLYEK+L + P++C +W K+
Sbjct: 592 ARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKF 646

BLAST of Sed0006761 vs. ExPASy Swiss-Prot
Match: P63154 (Crooked neck-like protein 1 OS=Mus musculus OX=10090 GN=Crnkl1 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.1e-148
Identity = 263/475 (55.37%), Postives = 335/475 (70.53%), Query Frame = 0

Query: 22  KLPRPIRVKNKNPAPIQITAEQILREARERH-EAEIRPPKRKITDSTELADYILCKRKEF 81
           ++P+  +VKNK PA +QITAEQ+LREA+ER  E    PP++KITD  EL DY L KRK F
Sbjct: 11  RIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTF 70

Query: 82  EDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEF 141
           ED +R+ R  I  WIKYA+WEES KE  RARS++ER L+VD RN TLW+KYAE+EMKN  
Sbjct: 71  EDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQ 130

Query: 142 INHARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIK 201
           +NHARN+WDRA   LPRV+Q W                 QVFERWM W P+++ W SYI 
Sbjct: 131 VNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYIN 190

Query: 202 FELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEE 261
           FELRY EVERAR ++ERFVL H  V  WI +A+FE K+   A AR VYERAVE   D+  
Sbjct: 191 FELRYKEVERARTIYERFVLVHPAVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHM 250

Query: 262 AEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDA 321
            E L VAFA+FEE+ KE ER R IYK++LD I K  A+++++ +  FE ++G++   ED 
Sbjct: 251 DEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQEAQELFKNYTIFEKKFGDRRGIEDI 310

Query: 322 I-------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWR 381
           I              NP +YD+WF+Y++L E+    + +REVYERAIANVPP +EKR+W+
Sbjct: 311 IVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEADTVREVYERAIANVPPIQEKRHWK 370

Query: 382 RYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKA 441
           RYIYLW+NYA+YEEL A D ERTR VY+  L LIPHKKF+FAK+WL  AQFE+RQ NL  
Sbjct: 371 RYIYLWVNYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWLYYAQFEIRQKNLPF 430

Query: 442 ARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKY 466
           AR+ LG +IG+ PK K+F+ YIE+ELQL   DRCRKLYEK+L + P++C +W K+
Sbjct: 431 ARRALGTSIGKCPKNKLFKGYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKF 485

BLAST of Sed0006761 vs. ExPASy Swiss-Prot
Match: P63155 (Crooked neck-like protein 1 OS=Rattus norvegicus OX=10116 GN=Crnkl1 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.1e-148
Identity = 263/475 (55.37%), Postives = 335/475 (70.53%), Query Frame = 0

Query: 22  KLPRPIRVKNKNPAPIQITAEQILREARERH-EAEIRPPKRKITDSTELADYILCKRKEF 81
           ++P+  +VKNK PA +QITAEQ+LREA+ER  E    PP++KITD  EL DY L KRK F
Sbjct: 11  RIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTF 70

Query: 82  EDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEF 141
           ED +R+ R  I  WIKYA+WEES KE  RARS++ER L+VD RN TLW+KYAE+EMKN  
Sbjct: 71  EDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQ 130

Query: 142 INHARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIK 201
           +NHARN+WDRA   LPRV+Q W                 QVFERWM W P+++ W SYI 
Sbjct: 131 VNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYIN 190

Query: 202 FELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEE 261
           FELRY EVERAR ++ERFVL H  V  WI +A+FE K+   A AR VYERAVE   D+  
Sbjct: 191 FELRYKEVERARTIYERFVLVHPAVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHM 250

Query: 262 AEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDA 321
            E L VAFA+FEE+ KE ER R IYK++LD I K  A+++++ +  FE ++G++   ED 
Sbjct: 251 DEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQEAQELFKNYTIFEKKFGDRRGIEDI 310

Query: 322 I-------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWR 381
           I              NP +YD+WF+Y++L E+    + +REVYERAIANVPP +EKR+W+
Sbjct: 311 IVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEADTVREVYERAIANVPPIQEKRHWK 370

Query: 382 RYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKA 441
           RYIYLW+NYA+YEEL A D ERTR VY+  L LIPHKKF+FAK+WL  AQFE+RQ NL  
Sbjct: 371 RYIYLWVNYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWLYYAQFEIRQKNLPF 430

Query: 442 ARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKY 466
           AR+ LG +IG+ PK K+F+ YIE+ELQL   DRCRKLYEK+L + P++C +W K+
Sbjct: 431 ARRALGTSIGKCPKNKLFKGYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKF 485

BLAST of Sed0006761 vs. ExPASy Swiss-Prot
Match: P17886 (Protein crooked neck OS=Drosophila melanogaster OX=7227 GN=crn PE=2 SV=2)

HSP 1 Score: 519.2 bits (1336), Expect = 5.1e-146
Identity = 250/475 (52.63%), Postives = 335/475 (70.53%), Query Frame = 0

Query: 22  KLPRPIRVKNKNPAPIQITAEQILREARERH-EAEIRPPKRKITDSTELADYILCKRKEF 81
           K+P+  +VKNK PA +QITAEQ+LREA+ER  E    PPK+KI+D  ELADY   KRK F
Sbjct: 6   KMPKVAKVKNKAPAEVQITAEQLLREAKERDLEILPPPPKQKISDPAELADYQQRKRKTF 65

Query: 82  EDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEF 141
           ED +R+ R  +  WIKYA+WEE Q+E  RARS+WER L+ ++RN TLW+KYAE+EMKN+ 
Sbjct: 66  EDNLRKNRMVVSHWIKYAQWEEQQQEIQRARSIWERALDNEHRNVTLWLKYAEMEMKNKQ 125

Query: 142 INHARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIK 201
           +NHARN+WDRA  ++PRV+Q W                 QVFERWM W P+++ W +Y+ 
Sbjct: 126 VNHARNLWDRAVTIMPRVNQFWYKYTYMEEMLENVAGARQVFERWMEWQPEEQAWQTYVN 185

Query: 202 FELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEE 261
           FELRY E++RAR ++ERFV  H  V  WI FA+FE  +G +  +R V+ERAVE   DD  
Sbjct: 186 FELRYKEIDRAREIYERFVYVHPDVKNWIKFARFEESHGFIHGSRRVFERAVEFFGDDYI 245

Query: 262 AEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDA 321
            E L +AFA FEE  KE +RAR IYK++LDH+PK R +++++ +   E +YG++A  ED 
Sbjct: 246 EERLFIAFARFEEGQKEHDRARIIYKYALDHLPKDRTQELFKAYTKHEKKYGDRAGIEDV 305

Query: 322 I-------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWR 381
           I              NP +YD+WF+Y++L E  G+ ++IRE YERAI+NVPPA EK +WR
Sbjct: 306 IVSKRKYQYEQEVAANPTNYDAWFDYLRLIEAEGDRDQIRETYERAISNVPPANEKNFWR 365

Query: 382 RYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKA 441
           RYIYLWINYA+YEEL A D ERTR +Y+ CL LIPHK+F+F+K+WLL AQFE+R   L+ 
Sbjct: 366 RYIYLWINYALYEELEAEDAERTRQIYKTCLELIPHKQFTFSKLWLLYAQFEIRCKELQR 425

Query: 442 ARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKY 466
           AR+ LG AIG  P++K+F  YI++E+QL   +RCR LYEK+L + P++C  W K+
Sbjct: 426 ARKALGLAIGMCPRDKLFRGYIDLEIQLREFERCRMLYEKFLEFGPENCVTWMKF 480

BLAST of Sed0006761 vs. ExPASy Swiss-Prot
Match: Q54XP4 (Crooked neck-like protein 1 OS=Dictyostelium discoideum OX=44689 GN=crnkl1 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 7.8e-139
Identity = 243/469 (51.81%), Postives = 323/469 (68.87%), Query Frame = 0

Query: 28  RVKNKNPAPIQITAEQILREARERHEAEIR-PPKRKITDSTELADYILCKRKEFEDLVRR 87
           +VKNK+ AP+QITAEQILR A E  +   + PPK+ ITD  EL DY L KR+++E L+ R
Sbjct: 11  KVKNKSAAPVQITAEQILRVAHENQQTLPKAPPKQVITDQEELEDYRLRKRQQYESLLGR 70

Query: 88  VRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARN 147
            R    ++IKYA WEESQK+  RARSV+ER L++D+R  T+WIKYAE+EMKN+ IN ARN
Sbjct: 71  NRKTAAIYIKYAAWEESQKDLTRARSVFERFLDIDHRIPTVWIKYAEMEMKNKNINLARN 130

Query: 148 VWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIKFELRYD 207
           +WDRA  LLPRV QLW                  +FERWM W P+ + W SY+KFE R  
Sbjct: 131 IWDRAVCLLPRVSQLWFKYTFMEDMLGNYPAARAIFERWMQWKPEPQAWNSYLKFEQRLK 190

Query: 208 EVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEEAEELLV 267
             E  R +FE+++L H  +  WI + KFE + G +  AR +++RA+E L +D   E+L +
Sbjct: 191 LFENTRLIFEKYILVHPYIKTWIKYTKFEERLGNIENARTIFQRAIEFLGEDGNDEQLFI 250

Query: 268 AFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDAI----- 327
           AFA+FEE  KEIERAR IYK+++DH+PK RAKD++  F  FE Q+G++   ED +     
Sbjct: 251 AFAKFEEKYKEIERARVIYKYAIDHVPKSRAKDLFDTFTNFEKQHGDRIGIEDVVLGKKR 310

Query: 328 --------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWRRYIYLW 387
                   KN  +YD WF+Y+++EE  G  E+ RE+YER+I N+PP  EK++W+RYIYLW
Sbjct: 311 FQYEEEIKKNSKNYDIWFDYLKMEEINGEIEKTREIYERSIGNLPPTNEKKHWKRYIYLW 370

Query: 388 INYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKAARQILG 447
           INYA++EEL + D+ER R VY +C+ LIPHK+FSF+KIW+L A FE+RQLNL  AR I G
Sbjct: 371 INYALFEELISKDMERARSVYSECIKLIPHKEFSFSKIWILYANFEIRQLNLDKARLIYG 430

Query: 448 NAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKY 466
            AIGR PK KIF++YI +E++L N DR R LYEKYL   P +C AW K+
Sbjct: 431 QAIGRNPKSKIFDQYIHLEIELGNFDRVRTLYEKYLEIMPDNCDAWCKF 479

BLAST of Sed0006761 vs. ExPASy TrEMBL
Match: A0A6J1HYQ8 (crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469305 PE=4 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 8.2e-232
Identity = 396/495 (80.00%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKFSLDHIPKGRA+D+
Sbjct: 241 IGKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFSLDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. ExPASy TrEMBL
Match: A0A6J1HYQ8 (crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469305 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.6e-17
Identity = 109/417 (26.14%), Postives = 179/417 (42.93%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA + +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAISNVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L   P+     
Sbjct: 444 NLNGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLVWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKI 408
           R +             +++ +WI+YA YE     D  +  ++    +    H +      
Sbjct: 564 RLLDRT----------KHLKVWISYAKYEASAMEDDSQLPELPEDKVQEYLHAR------ 623

Query: 409 WLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRCR 468
                    +Q  ++ AR++   AI      AP+ K     + E+++ +E     +    
Sbjct: 624 ---------KQQCIQQARRVFEKAITYYRNSAPELKEERAMLLEEWLNMETSFGELGDVS 683

Query: 469 KLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
            +  K      K     ++ G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 LVQSKLPKKLKKRRQIASEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 812.8 bits (2098), Expect = 8.2e-232
Identity = 398/495 (80.40%), Postives = 431/495 (87.07%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWNI VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNISVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           +FERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 IFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           VAKARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 VAKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDI 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAIANVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAIANVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHGKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL+ ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLQGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. ExPASy TrEMBL
Match: A0A6J1DG47 (crooked neck-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111020524 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.5e-18
Identity = 112/418 (26.79%), Postives = 182/418 (43.54%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA A +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAIANVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHGKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L+  P+     
Sbjct: 444 NLQGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLEWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKF-SFAK 408
           R +             +++ +WI+YA +E     D  R        LS +P      + +
Sbjct: 564 RLLDRT----------KHLKVWISYAKFEASAMEDDSR--------LSELPEDNVQEYLQ 623

Query: 409 IWLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRC 468
            W        ++  ++ AR++   AI      AP+ K     + E+++ +E    ++   
Sbjct: 624 SW--------KKQCIQHARRVFEKAITYYRNSAPELKEERAMLLEEWLNMEASFGDLGDV 683

Query: 469 RKLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
             +  K      K      + G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 SLVQSKLPKKLKKRRQIATEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 811.6 bits (2095), Expect = 1.8e-231
Identity = 395/495 (79.80%), Postives = 430/495 (86.87%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWN+ VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNVSVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           + KARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 ITKARNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEET GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAI+NVPPAEEKRYW+RYIYLWINYA+YEEL+A D+ERTRDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAISNVPPAEEKRYWQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNL  ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLNGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. ExPASy TrEMBL
Match: A0A6J1HLN2 (crooked neck-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111464720 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.7e-17
Identity = 114/417 (27.34%), Postives = 183/417 (43.88%), Query Frame = 0

Query: 109 RARSVWERVLEVDNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLWQVFERW 168
           + R  +E  +  +  N+  W  Y  +E         R V++RA + +P  ++     +R+
Sbjct: 324 KRRFQYEEEVRKNPLNYDSWFDYIRLEETAGNKERIREVYERAISNVPPAEE-----KRY 383

Query: 169 MGWMPDQEGWLSYIKF-ELRYDEVERARGVFERF--VLCHNKVG---AWIGFAKFEMKNG 228
             W      W++Y  + EL   +VER R V+     ++ H K      W+  A+FE++  
Sbjct: 384 --WQRYIYLWINYALYEELDAADVERTRDVYRECLNLIPHAKFSFAKIWLLAAQFEIRQL 443

Query: 229 EVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPK----- 288
            +  AR +   A+ +   D    ++   + E E     I+R R +Y+  L   P+     
Sbjct: 444 NLNGARQILGNAIGRAPKD----KIFKKYIEIELQLGNIDRCRKLYEKYLVWSPENCYAW 503

Query: 289 GRAKDMYRKFVAFEMQYGNKAETEDAIKNP-LDYDS--WFEYIQLEETTGNTERIREVYE 348
            +  ++ R     E     ++  E AI  P LD     W  YI  E +    ER RE+YE
Sbjct: 504 SKYAELERSLCETERA---RSIFELAIAQPALDMPELLWKAYIDFEISEHEFERTRELYE 563

Query: 349 RAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKI 408
           R +             +++ +WI+YA YE     D  +        LS +P  K    + 
Sbjct: 564 RLLDRT----------KHLKVWISYAKYEASAMEDDSQ--------LSELPEDK---VQE 623

Query: 409 WLLAAQFELRQLNLKAARQILGNAI----GRAPKEK-----IFEKYIEIELQLENIDRCR 468
           +L A     +Q  ++ AR++   AI      AP+ K     + E+++ +E     +    
Sbjct: 624 YLRAR----KQQCIQQARRVFEKAITYYRNSAPELKEERAMLLEEWLNMETSFGELGDVS 683

Query: 469 KLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWKKQKVD 502
            +  K      K     ++ G AGF E+ DYL P ETQ TNLKILEAAYRWKKQKVD
Sbjct: 684 LVQSKLPKKLKKRRQIASEDGPAGFEEYIDYLFPEETQTTNLKILEAAYRWKKQKVD 701


HSP 2 Score: 808.5 bits (2087), Expect = 1.5e-230
Identity = 394/495 (79.60%), Postives = 433/495 (87.47%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MSS KD+D +LGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSSAKDADPSLGYLTRKDTEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITDSTELADY L KRKEFEDL+RRVRWNI VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDSTELADYRLRKRKEFEDLIRRVRWNISVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           +FERWMGWMPDQ+GWLSYIKFELRY+E+ERARG+FERFV CH KV AWI +AKFEMKNGE
Sbjct: 181 IFERWMGWMPDQQGWLSYIKFELRYNEIERARGIFERFVQCHPKVSAWIRYAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           VA+ARNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 VARARNVYERAVEKLADDEEAEQLFVAFAEFEERCKESERARCIYKFALDHIPKGRAEDL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEE+ GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEDEVRKNPLNYDSWFDYIRLEESVGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAIANVPPAEEKRYW+RYIYLWINYA+YEEL+A DIERTRDVYR+C++LIPHKKFS
Sbjct: 361 EVYERAIANVPPAEEKRYWQRYIYLWINYALYEELDAEDIERTRDVYRECMNLIPHKKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNLK+ARQILGNAIG+APK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLKSARQILGNAIGKAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. ExPASy TrEMBL
Match: A0A6J1I1K2 (crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111468607 PE=4 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.5e-230
Identity = 395/495 (79.80%), Postives = 428/495 (86.46%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           MS  KD+D TLGYLTR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK
Sbjct: 1   MSYSKDADPTLGYLTRKDAEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPK 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           +KITD TELADY L KRKEFEDL+RRVRWNI VWIKYA+WEESQK+F+RARSVWER LEV
Sbjct: 61  QKITDPTELADYRLRKRKEFEDLIRRVRWNISVWIKYAQWEESQKDFNRARSVWERALEV 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
           D RNHTLW+KYAEVEMKN+FINHARNVWDRA  LLPRVDQLW                 Q
Sbjct: 121 DYRNHTLWLKYAEVEMKNKFINHARNVWDRAVTLLPRVDQLWYKYIHMEEMLGNVAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           VFERWMGWMPDQ+GWLSYIKFELRY+EVERARG+FERFV CH KVGAWI FAKFEMKNGE
Sbjct: 181 VFERWMGWMPDQQGWLSYIKFELRYNEVERARGIFERFVQCHPKVGAWIRFAKFEMKNGE 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           VAK+RNVYERAVEKL DDEEAE+L VAFAEFEE CKE ERARCIYKF+LDHIPKGRA+D+
Sbjct: 241 VAKSRNVYERAVEKLADDEEAEQLFVAFAEFEERCKETERARCIYKFALDHIPKGRAEDI 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           YRKFVAFE QYG+K   EDAI             KNPL+YDSWF+YI+LEE  GN ERIR
Sbjct: 301 YRKFVAFEKQYGDKEGIEDAIVGKRRFQYEEEVRKNPLNYDSWFDYIRLEEAAGNKERIR 360

Query: 361 EVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFS 420
           EVYERAIANVPPAEEKRYW+RYIYLWINYA+YEEL+A D+E  RDVYR+CL+LIPH KFS
Sbjct: 361 EVYERAIANVPPAEEKRYWQRYIYLWINYALYEELDAADVEHARDVYRECLNLIPHGKFS 420

Query: 421 FAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEK 466
           FAKIWLLAAQFE+RQLNLK ARQILGNAIGRAPK+KIF+KYIEIELQL NIDRCRKLYEK
Sbjct: 421 FAKIWLLAAQFEIRQLNLKGARQILGNAIGRAPKDKIFKKYIEIELQLGNIDRCRKLYEK 480

BLAST of Sed0006761 vs. TAIR 10
Match: AT5G41770.1 (crooked neck protein, putative / cell cycle protein, putative )

HSP 1 Score: 748.4 bits (1931), Expect = 3.6e-216
Identity = 361/494 (73.08%), Postives = 413/494 (83.60%), Query Frame = 0

Query: 2   SSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPKR 61
           S  KDSD+TLGY+TR D  VKLPRP RVKNK PAPIQITAEQILREARER EAEIRPPK+
Sbjct: 3   SGGKDSDRTLGYMTRKDTEVKLPRPTRVKNKTPAPIQITAEQILREARERQEAEIRPPKQ 62

Query: 62  KITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVD 121
           KITDSTEL+DY L +RKEFED +RR RWNI VW+KYA+WEESQK++ RARSVWER +E D
Sbjct: 63  KITDSTELSDYRLRRRKEFEDQIRRARWNIQVWVKYAQWEESQKDYARARSVWERAIEGD 122

Query: 122 NRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------QV 181
            RNHTLW+KYAE EMKN+F+N ARNVWDRA  LLPRVDQLW                 Q+
Sbjct: 123 YRNHTLWLKYAEFEMKNKFVNSARNVWDRAVTLLPRVDQLWYKYIHMEEILGNIAGARQI 182

Query: 182 FERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEV 241
           FERWM W PDQ+GWLS+IKFELRY+E+ERAR ++ERFVLCH KV A+I +AKFEMK GEV
Sbjct: 183 FERWMDWSPDQQGWLSFIKFELRYNEIERARTIYERFVLCHPKVSAYIRYAKFEMKGGEV 242

Query: 242 AKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMY 301
           A+ R+VYERA EKL DDEEAE L VAFAEFEE CKE+ERAR IYKF+LDHIPKGRA+D+Y
Sbjct: 243 ARCRSVYERATEKLADDEEAEILFVAFAEFEERCKEVERARFIYKFALDHIPKGRAEDLY 302

Query: 302 RKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIRE 361
           RKFVAFE QYG+K   EDAI             K+P +YDSWF+Y++LEE+ GN +RIRE
Sbjct: 303 RKFVAFEKQYGDKEGIEDAIVGKRRFQYEDEVRKSPSNYDSWFDYVRLEESVGNKDRIRE 362

Query: 362 VYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSF 421
           +YERAIANVPPAEEKRYW+RYIYLWINYA++EE+   DIERTRDVYR+CL LIPH KFSF
Sbjct: 363 IYERAIANVPPAEEKRYWQRYIYLWINYALFEEIETEDIERTRDVYRECLKLIPHSKFSF 422

Query: 422 AKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKY 466
           AKIWLLAAQFE+RQLNL  ARQILGNAIG+APK+KIF+KYIEIELQL N+DRCRKLYE+Y
Sbjct: 423 AKIWLLAAQFEIRQLNLTGARQILGNAIGKAPKDKIFKKYIEIELQLGNMDRCRKLYERY 482


HSP 2 Score: 70.5 bits (171), Expect = 4.4e-12
Identity = 86/364 (23.63%), Postives = 152/364 (41.76%), Query Frame = 0

Query: 159 DQLWQVFERWMGWMPDQEG----------WLSYIKF-ELRYDEVERARGVFERF--VLCH 218
           D++ +++ER +  +P  E           W++Y  F E+  +++ER R V+     ++ H
Sbjct: 358 DRIREIYERAIANVPPAEEKRYWQRYIYLWINYALFEEIETEDIERTRDVYRECLKLIPH 417

Query: 219 NKVG---AWIGFAKFEMKNGEVAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIE 278
           +K      W+  A+FE++   +  AR +   A+ K   D    ++   + E E     ++
Sbjct: 418 SKFSFAKIWLLAAQFEIRQLNLTGARQILGNAIGKAPKD----KIFKKYIEIELQLGNMD 477

Query: 279 RARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGN----KAETEDAIKNPLDYDSWFEYIQ 338
           R R +Y+  L+  P+      + K+   E         +A  E AI  P         + 
Sbjct: 478 RCRKLYERYLEWSPENCY--AWSKYAELERSLVETERARAIFELAISQPA--------LD 537

Query: 339 LEETTGNTERIREVYERAIANVPPAEEKRYWR-RYIYLWINYAVYEELNAGDIERTRDVY 398
           + E         E+ E  +       E+   R ++  +W+++A +E   A   E   +  
Sbjct: 538 MPELLWKAYIDFEISEGELERTRALYERLLDRTKHYKVWVSFAKFEASAAELEEDENEDE 597

Query: 399 RQCLSLIPHKKFSFAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQ 458
            Q   +I HKK    +     A F+      K +   L     +  +  + E ++ +E  
Sbjct: 598 DQEEDVIEHKKDCIKR---ARAIFDRANTYYKDSTPEL-----KEERATLLEDWLNMESS 657

Query: 459 LENIDRCRKLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPPNETQPTNLKILEAAYRWK 501
             N+     +  K      K      + G   + E+ DYL P E+Q TNLKILEAAY+WK
Sbjct: 658 FGNLGDVSIVQSKLPKKLKKRKAITREDGSTEYEEYIDYLYPEESQTTNLKILEAAYKWK 699

BLAST of Sed0006761 vs. TAIR 10
Match: AT5G45990.1 (crooked neck protein, putative / cell cycle protein, putative )

HSP 1 Score: 694.1 bits (1790), Expect = 8.2e-200
Identity = 334/483 (69.15%), Postives = 395/483 (81.78%), Query Frame = 0

Query: 14  LTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPKRKITDSTELADYI 73
           +T  D  VKLPR  RVKNK PAP+QITAEQILREARER EAEIRPPK+KITDSTEL+DY 
Sbjct: 1   MTGKDAEVKLPRTTRVKNKTPAPVQITAEQILREARERQEAEIRPPKQKITDSTELSDYR 60

Query: 74  LCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAE 133
           L +RKEFED +RR RWNI VW+KYA+WEESQ ++ RARSVWER LE + RNHTLW+KYAE
Sbjct: 61  LRRRKEFEDQIRRARWNIQVWVKYAKWEESQMDYARARSVWERALEGEYRNHTLWVKYAE 120

Query: 134 VEMKNEFINHARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQE 193
            EMKN+F+N+ARNVWDR+  LLPRVDQLW                 Q+FERWM W PDQ+
Sbjct: 121 FEMKNKFVNNARNVWDRSVTLLPRVDQLWEKYIYMEEKLGNVTGARQIFERWMNWSPDQK 180

Query: 194 GWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMK-NGEVAKARNVYERAV 253
            WL +IKFELRY+E+ERAR ++ERFVLCH KV A+I +AKFEMK  G+V  AR VYERAV
Sbjct: 181 AWLCFIKFELRYNEIERARSIYERFVLCHPKVSAFIRYAKFEMKRGGQVKLAREVYERAV 240

Query: 254 EKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYG 313
           +KL +DEEAE L V+FAEFEE CKE+ERAR IYKF+LDHI KGRA+++Y+KFVAFE QYG
Sbjct: 241 DKLANDEEAEILFVSFAEFEERCKEVERARFIYKFALDHIRKGRAEELYKKFVAFEKQYG 300

Query: 314 NKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPP 373
           +K   EDAI             KNPL+YDSWF+Y++LEE+ GN +RIRE+YERAIANVPP
Sbjct: 301 DKEGIEDAIVGKKRFEYEDEVSKNPLNYDSWFDYVRLEESVGNKDRIREIYERAIANVPP 360

Query: 374 AEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAKIWLLAAQFE 433
           A+EKR+W+RYIYLWINYA+YEE+   D+ERTRDVYR+CL LIPH KFSFAKIWLLAA++E
Sbjct: 361 AQEKRFWQRYIYLWINYALYEEIETKDVERTRDVYRECLKLIPHTKFSFAKIWLLAAEYE 420

Query: 434 LRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAW 466
           +RQLNL  ARQILGNAIG+APK KIF+KYIE+EL+L NIDRCRKLYE++L WSP++CYAW
Sbjct: 421 IRQLNLTGARQILGNAIGKAPKVKIFKKYIEMELKLVNIDRCRKLYERFLEWSPENCYAW 480


HSP 2 Score: 95.1 bits (235), Expect = 1.7e-19
Identity = 100/441 (22.68%), Postives = 189/441 (42.86%), Query Frame = 0

Query: 93  VWIKYARWEESQKEFDRARSVWERVLE--VDNRNHTLWIKYAEVEMKNEFINHARNVWDR 152
           +++ +A +EE  KE +RAR +++  L+     R   L+ K+   E +         + D 
Sbjct: 252 LFVSFAEFEERCKEVERARFIYKFALDHIRKGRAEELYKKFVAFEKQ---YGDKEGIED- 311

Query: 153 AAALLPRVDQLWQVFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFV-------- 212
           A     R +   +V +  + +    + W  Y++ E      +R R ++ER +        
Sbjct: 312 AIVGKKRFEYEDEVSKNPLNY----DSWFDYVRLEESVGNKDRIREIYERAIANVPPAQE 371

Query: 213 --LCHNKVGAWIGFAKF-EMKNGEVAKARNVYERAVEKLVDDE-EAEELLVAFAEFEESC 272
                  +  WI +A + E++  +V + R+VY   ++ +   +    ++ +  AE+E   
Sbjct: 372 KRFWQRYIYLWINYALYEEIETKDVERTRDVYRECLKLIPHTKFSFAKIWLLAAEYEIRQ 431

Query: 273 KEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDAIKNPLDYD-----SW 332
             +  AR I   ++   PK +   +++K++  E++  N        +  L++      +W
Sbjct: 432 LNLTGARQILGNAIGKAPKVK---IFKKYIEMELKLVNIDRCRKLYERFLEWSPENCYAW 491

Query: 333 FEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIERT 392
             Y + E +   TER R ++E AI+       +  W+ YI          E++ G+ E+T
Sbjct: 492 RNYAEFEISLAETERARAIFELAISQPALDMPELLWKTYIDF--------EISEGEFEKT 551

Query: 393 RDVYRQCLSLIPHKKFSFAKIWLLAAQFEL-----RQLNLKAARQILGNAIG-------- 452
           R +Y + L    H      K+W+  A+FE      ++  +K+AR I   A          
Sbjct: 552 RALYERLLDRTKH-----CKVWISFAKFEASASEHKEDGIKSARVIFDRANTYYKDTTPE 611

Query: 453 -RAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYLPP 500
               +  + E ++ +E     +     +  K      K      + G   + E+ DYL P
Sbjct: 612 LEEERATLLEDWLNMETGFGELGDVSVVQSKLPKKLKKRKMTSREDGSTEYEEYFDYLFP 668

BLAST of Sed0006761 vs. TAIR 10
Match: AT3G13210.1 (crooked neck protein, putative / cell cycle protein, putative )

HSP 1 Score: 576.6 bits (1485), Expect = 1.9e-164
Identity = 298/497 (59.96%), Postives = 352/497 (70.82%), Query Frame = 0

Query: 1   MSSFKDSDQTLGYLTRNDLNVKLPRPIRVKNKNPAPIQITAEQILREARERHEAEIRPPK 60
           M+S KDSD+ LGY+TR D  +KLPR  +VKNK PAPIQITAEQILREARER EAE RPP 
Sbjct: 1   MASGKDSDRNLGYMTRKDAELKLPRMTQVKNKTPAPIQITAEQILREARERQEAEFRPPN 60

Query: 61  RKITDSTELADYILCKRKEFEDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEV 120
           + ITDS EL+DY L +RKEFED +RR R N  V                           
Sbjct: 61  QTITDSAELSDYRLRRRKEFEDQIRRARLNTQV--------------------------- 120

Query: 121 DNRNHTLWIKYAEVEMKNEFINHARNVWDRAAALLPRVDQLW-----------------Q 180
                  W+KYA+ EMKN+ +N ARNVWDRA +LLPRVDQLW                 Q
Sbjct: 121 -------WVKYADFEMKNKSVNEARNVWDRAVSLLPRVDQLWYKFIHMEEKLGNIAGARQ 180

Query: 181 VFERWMGWMPDQEGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGE 240
           + ERW+   PDQ+ WL +IKFEL+Y+E+E AR ++ERFVLCH KV A+I +AKFEMK+G+
Sbjct: 181 ILERWIHCSPDQQAWLCFIKFELKYNEIECARSIYERFVLCHPKVSAYIRYAKFEMKHGQ 240

Query: 241 VAKARNVYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDM 300
           V  A  V+ERA ++L DDEEAE L VAFAEFEE           YKF+LD IPKGRA+++
Sbjct: 241 VELAMKVFERAKKELADDEEAEILFVAFAEFEEQ----------YKFALDQIPKGRAENL 300

Query: 301 YRKFVAFEMQYGNKAETEDAI-------------KNPLDYDSWFEYIQLEETTGNTERIR 360
           Y KFVAFE Q G+K   EDAI             KNPL+YDSWF++++LEET GN +RIR
Sbjct: 301 YSKFVAFEKQNGDKEGIEDAIIGKRRCQYEDEVRKNPLNYDSWFDFVRLEETVGNKDRIR 360

Query: 361 EVYERAIANVPP--AEEKRYWRRYIYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKK 420
           E+YERA+ANVPP  A+EKRYW+RYIYLWINYA + E+   D+E TRDVYR CL LIPH K
Sbjct: 361 EIYERAVANVPPPEAQEKRYWQRYIYLWINYAFFAEMVTEDVESTRDVYRACLKLIPHSK 420

Query: 421 FSFAKIWLLAAQFELRQLNLKAARQILGNAIGRAPKEKIFEKYIEIELQLENIDRCRKLY 466
           FSFAKIWLLAAQ E+RQLNL  ARQILGNAIG+APK+KIF+KYIEIELQL NIDRCRKLY
Sbjct: 421 FSFAKIWLLAAQHEIRQLNLTGARQILGNAIGKAPKDKIFKKYIEIELQLRNIDRCRKLY 453


HSP 2 Score: 65.9 bits (159), Expect = 1.1e-10
Identity = 55/204 (26.96%), Postives = 90/204 (44.12%), Query Frame = 0

Query: 312 SWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWRRYIYLWINYAVYEELNAGDIE 371
           +W +Y + E +   TER R ++E AI+       +  W+ YI   I+    E   A   E
Sbjct: 449 AWRKYAEFEMSLAETERTRAIFELAISQPALDMPELLWKTYIDFEISEGELERTRA-LYE 508

Query: 372 RTRDVYRQCLSLIPHKKFSFAKIWLLAAQ-----FELRQLNLKAARQILGNAIG------ 431
           R  D  + C   +   KF  +       +      E ++  +K AR+I   A        
Sbjct: 509 RLLDRTKHCKVWVDFAKFEASAAEHKEDEEEEDAIERKKDGIKRAREIFDRANTYNKDST 568

Query: 432 ---RAPKEKIFEKYIEIELQLENIDRCRKLYEKYLLWSPKDCYAWNKYGLAGF-EHTDYL 491
              +  +  + E ++ +E     +   R +  K      K   +  + G   + E+TDYL
Sbjct: 569 PELKEERAMLLEDWLNMETGFGKLGDVRVVQSKLPKKVKKRKLSSREDGCTEYEEYTDYL 628

Query: 492 PPNETQPTNLKILEAAYRWKKQKV 501
            P E++ T+LKILEAA++WKKQKV
Sbjct: 629 FPEESETTSLKILEAAHKWKKQKV 651

BLAST of Sed0006761 vs. TAIR 10
Match: AT3G51110.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.3e-144
Identity = 252/401 (62.84%), Postives = 311/401 (77.56%), Query Frame = 0

Query: 26  PIRVKNKNPAPIQITAEQILREARERHEAEI-RPPKRKITDSTELADYILCKRKEFEDLV 85
           P  VKNK PAP+QITAEQ+L+EARER ++ I RPPK+KITDS ELA+Y L +RKEFED +
Sbjct: 5   PRMVKNKTPAPVQITAEQVLKEAREREDSRILRPPKQKITDSDELAEYRLRRRKEFEDQI 64

Query: 86  RRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDN-RNHTLWIKYAEVEMKNEFINH 145
           R  + N  VW++YA WEESQK+ DRARSVWER LE ++ RNHTLW+KYAE EM+N+ +NH
Sbjct: 65  RGAKTNSQVWVRYADWEESQKDHDRARSVWERALEDESYRNHTLWLKYAEFEMRNKSVNH 124

Query: 146 ARNVWDRAAALLPRVDQLW-----------------QVFERWMGWMPDQEGWLSYIKFEL 205
           ARNVWDRA  +LPRVDQ W                 ++FERWM W PDQ+ WL +IKFEL
Sbjct: 125 ARNVWDRAVKILPRVDQFWYKYIHMEEILGNIDGARKIFERWMDWSPDQQAWLCFIKFEL 184

Query: 206 RYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVAKARNVYERAVEKLVD-DEEAE 265
           RY+E+ER+R ++ERFVLCH K  ++I +AKFEMKN +V+ AR VYERA+E L D +EEAE
Sbjct: 185 RYNEIERSRSIYERFVLCHPKASSFIRYAKFEMKNSQVSLARIVYERAIEMLKDVEEEAE 244

Query: 266 ELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVAFEMQYGNKAETEDAI- 325
            + VAFAEFEE CKE+ERAR +YK++LDHIPKGRA+D+Y+KFVAFE QYGNK   +DAI 
Sbjct: 245 MIFVAFAEFEELCKEVERARFLYKYALDHIPKGRAEDLYKKFVAFEKQYGNKEGIDDAIV 304

Query: 326 ------------KNPLDYDSWFEYIQLEETTGNTERIREVYERAIANVPPAEEKRYWRRY 385
                       KNPL+YDSWF+YI LEET G+ +RIREVYERAIANVP AEEKRYW+RY
Sbjct: 305 GRRKLQYEGEVRKNPLNYDSWFDYISLEETLGDKDRIREVYERAIANVPLAEEKRYWQRY 364

Query: 386 IYLWINYAVYEELNAGDIERTRDVYRQCLSLIPHKKFSFAK 394
           IYLWI+YA++EE+ A D+ERTR VYR+CL+LIP  +  F +
Sbjct: 365 IYLWIDYALFEEILAEDVERTRAVYRECLNLIPSLQILFCQ 405

BLAST of Sed0006761 vs. TAIR 10
Match: AT5G28740.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 6.8e-13
Identity = 59/248 (23.79%), Postives = 110/248 (44.35%), Query Frame = 0

Query: 81  EDLVRRVRWNIGVWIKYARWEESQKEFDRARSVWERVLEVDNRNHTLWIKYAEVEMKNEF 140
           E +  ++  ++ +W  Y   EES    +  R+V+E++L++      + + YA +  +N++
Sbjct: 505 EPVQMKLHRSLRLWSFYVDLEESLGTLESTRAVYEKILDLRIATPQIIMNYAFLLEENKY 564

Query: 141 INHARNVWDRAAALL--PRVDQLW--------------------QVFERWMGWMPD---Q 200
              A  V++R   +   P V  +W                    ++FE  +   P    +
Sbjct: 565 FEDAFKVYERGVKIFKYPHVKDIWVTYLTKFVKRYGKTKLERARELFEHAVSMAPSDAVR 624

Query: 201 EGWLSYIKFELRYDEVERARGVFERFVLCHNKVGAWIGFAKFEMKNGEVA------KARN 260
             +L Y K E  Y   +RA  V+E       KV        +E+     A      + R 
Sbjct: 625 TLYLQYAKLEEDYGLAKRAMKVYEE---ATKKVPEGQKLEMYEIYISRAAEIFGVPRTRE 684

Query: 261 VYERAVEKLVDDEEAEELLVAFAEFEESCKEIERARCIYKFSLDHIPKGRAKDMYRKFVA 298
           +YE+A+E  +  ++ + + + FAE E S  EI+RAR +YK+S          + + K+  
Sbjct: 685 IYEQAIESGLPHKDVKIMCIKFAELERSLGEIDRARALYKYSSQFADPRSDPEFWNKWHE 744

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152908.11.7e-23180.40crooked neck-like protein 1 [Momordica charantia] >XP_022152909.1 crooked neck-l... [more]
XP_022152908.11.1e-1726.79crooked neck-like protein 1 [Momordica charantia] >XP_022152909.1 crooked neck-l... [more]
XP_023519624.15.6e-1727.34crooked neck-like protein 1 [Cucurbita pepo subsp. pepo][more]
XP_022970297.19.5e-1726.14crooked neck-like protein 1 [Cucurbita maxima] >XP_022970298.1 crooked neck-like... [more]
XP_022964730.15.6e-1727.34crooked neck-like protein 1 [Cucurbita moschata] >XP_022964731.1 crooked neck-li... [more]

Pages

Match NameE-valueIdentityDescription
Q9BZJ02.4e-14855.58Crooked neck-like protein 1 OS=Homo sapiens OX=9606 GN=CRNKL1 PE=1 SV=4[more]
P631547.1e-14855.37Crooked neck-like protein 1 OS=Mus musculus OX=10090 GN=Crnkl1 PE=1 SV=1[more]
P631557.1e-14855.37Crooked neck-like protein 1 OS=Rattus norvegicus OX=10116 GN=Crnkl1 PE=2 SV=1[more]
P178865.1e-14652.63Protein crooked neck OS=Drosophila melanogaster OX=7227 GN=crn PE=2 SV=2[more]
Q54XP47.8e-13951.81Crooked neck-like protein 1 OS=Dictyostelium discoideum OX=44689 GN=crnkl1 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HYQ88.2e-23280.00crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469305 PE=4 SV=... [more]
A0A6J1HYQ84.6e-1726.14crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111469305 PE=4 SV=... [more]
A0A6J1DG475.5e-1826.79crooked neck-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111020524 PE=4 ... [more]
A0A6J1HLN22.7e-1727.34crooked neck-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111464720 PE=4 S... [more]
A0A6J1I1K24.5e-23079.80crooked neck-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111468607 PE=4 SV=... [more]

Pages

Match NameE-valueIdentityDescription
AT5G41770.13.6e-21673.08crooked neck protein, putative / cell cycle protein, putative [more]
AT5G45990.18.2e-20069.15crooked neck protein, putative / cell cycle protein, putative [more]
AT3G13210.11.9e-16459.96crooked neck protein, putative / cell cycle protein, putative [more]
AT3G51110.11.3e-14462.84Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G28740.16.8e-1323.79Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 244..264
NoneNo IPR availablePANTHERPTHR11246:SF28SUBFAMILY NOT NAMEDcoord: 21..466
IPR003107HAT (Half-A-TPR) repeatSMARTSM00386hat_new_1coord: 259..293
e-value: 2.4
score: 17.1
coord: 156..187
e-value: 60.0
score: 9.7
coord: 71..103
e-value: 0.79
score: 18.8
coord: 324..358
e-value: 2.8E-6
score: 36.9
coord: 368..404
e-value: 4.1E-6
score: 36.3
coord: 105..137
e-value: 2.3E-5
score: 33.8
coord: 406..437
e-value: 260.0
score: 5.0
coord: 439..471
e-value: 48.0
score: 10.4
coord: 222..257
e-value: 0.0066
score: 25.7
coord: 189..220
e-value: 0.0056
score: 25.9
IPR003107HAT (Half-A-TPR) repeatPFAMPF02184HATcoord: 105..135
e-value: 4.0E-5
score: 23.5
coord: 190..218
e-value: 3.3E-10
score: 39.7
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 304..474
e-value: 3.5E-33
score: 117.0
coord: 67..159
e-value: 4.0E-16
score: 61.0
coord: 160..303
e-value: 3.7E-30
score: 107.1
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 81..175
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 339..466
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 163..248
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 301..353
IPR045075Pre-mRNA-splicing factor Syf1-likePANTHERPTHR11246PRE-MRNA SPLICING FACTORcoord: 21..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0006761.1Sed0006761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
biological_process GO:0006396 RNA processing
molecular_function GO:0005515 protein binding