Sed0006741 (gene) Chayote v1

Overview
NameSed0006741
Typegene
OrganismSechium edule (Chayote v1)
DescriptionHydroxymethylbilane synthase
LocationLG05: 25545084 .. 25548774 (-)
RNA-Seq ExpressionSed0006741
SyntenySed0006741
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATGGATGAGAATCCTATCCGCCAACGCGAATGCAGACATTTGCCCACTGGGTCTCATCAATAACACAATCCCTTCCTTCTAAGCTAACCCTCTCTTCCCCATTTCCCCAATTCCCCCTTTTTTCTTCTAATTCCTCTCTCCAATGGCGGCTTTCTCTTCCCCTTCAATCAGCCACACTCCCATGGCTCGCCCCTGCAATGTTGGCTCCCTTTCCCTTCTTGGGTTCTCCTCTATTTCCCTTAAGCCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGGTTGGCCTCGTACGAGCCTCCGTTGCGGTGGAGCAGCAGGCAGAGAAGACCAAGGTCGCCCTCCTCAGAATTGGCACCAGAGGAAGGTGATTTCTTGGATTTTGCCCTTTTCTGATTTCTGGGTCGTTTTTGGATTCCGGTTTTGTTTCATGTTTTTGGGTTGGCTTTAGTTTTTAGTTTTGAGTTTTTGGCTTGTTGGAGTTTTTTTGTTTTCGAAGGGTTTGGATCAGCTTTCAAGTTTCATCTATGATTTGGTTTTGATATTTTGTAGAATTGTGAAAATGTTCAGTTTGATGTTTTTGGGATGTCTTTAGTTTCTAATTTTGAGTTTGTGATTGATTTGAGTTTGTTTAATGTGGGGCATTTGAGTTTTTTGTTGAAGGTTTTGGATCAGTTCAAGTTATGCCTTCAAGTTTCAACTGTGATTTAGTTTTTGATAACTTGTAGAATTGTTAAAAAGTTCGGTTAGATGTTCTTGGGACGTCTTTAGTTTCTAATTTTGAGTATGTGGCTGATTTGAGTTTGAATTTGGGGCATTTGTGTTGTTTGTTGAAGGGTTTGGATTAGTTGAAGTTATGCTTTCAAGTCTCAACTGTGATTTGGTTTTTGATATCTTGGAGAGTTATGAAATAAAATTCAGTTTCTTCATCATGTTTGTTCTAGAATTCTGTATTCTGGCTCTTTCCAAACTCGTATGTGTCAGTCTTGTTCAACTCAAGAAGTACTTTTCTTGCTGCTTGCCAGGGGTGAAGTAGATGTCTTTATTGGTGGTGTGGTAGGTGGTGAGAGTTAATTCAATGTCTAGTATTTTTCACTCAGACTTTGAATTTGAATGGACCTTATAGATTGTTGATGTTTCCTTTTTTTTAGTTCAACAACATTTGACATATTCGAACCTATGATAACTTGGTCATAAGCACATATTTAATGACTCTTGAGCAATGCTCTTGTTGGAGATGGTTGAGGATTCCTTTTGTAGGCTTGTATTTTGTATGCCCTTATAATATGTTTGCTTTTCAAGAATGGAAGTTGCGTTTCTTTTTCATGGGTGTACATCAGATTATCAAGTATGCTAAATGGTGGAAAATGAACCTAACCAAAGATGAAAAGATGCCTTACAATTGTGAGAGCCTCCCCATTTATTTTCAGAAAAAGTATTCATCATGCTGATCATGTCATCAATCATGGTTTTGGTATTGTTTTATTTTACTGTCTAGATGTTAGGATTAATCTGCTACTGTTATAGAGTCGAATGAGATCTTTATTAGAAGAGGGAGTCTACGCTTGTAGGAACTACAGGGCTTGTAATTTTCTTATCGGAACCGGGAGCTATGTCTGTCAACATAATGTACTTTGTTCTCTAGCTGATTATATGCTTGTTTGATCTTCTGCTTTATTGCTTTATGTTCTGCTGTGTCATATGCACTTCTCTGTGTGTAGGACAGCCCGTTAGCACTTGCCCAGGCTCACGAGACGAGAAACAAACTCATGGCTATCCATCCCGAGCTTGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTCGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGACGCACTTATTAATGGCGACATAGACATTGCCGTTCACTCTATGAAAGATGTTCCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCGAGGGAAGATGTTCGCGATGCGTTCATTTCTCTTAGTGCGGGGTCACTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTAAGTATATTGTGTTATTTAATGGAAAGACGCCGAAAGTTTATGTTCTACGTTTACATCACTATAACCATTGAAATAAGCTCCTCAAGGATACGATAGCAAAAATGTCCCTTTGGTTCTCTTTCTACATTGGTTGACCTTTTAAAATGTTCTTCTACATTTCTGTAGTCTGTGTTATCTCAAATTCTTTCATTTTTTTCCTTTCTTATTGATTTCTGATACTATGAAGAGTTGTCACTGTTATGTTTCACGATTATCGATCACTTTCTTATACGAAAAAACTTGAAGATTACTTGCTGCCTCCACCTCACCTTGAATTAAGTATTCCCGTCCTTTGATGGAACGATTTATGACTCTGTGATTTTGTAGATGTTAAAGAGGCTTATATCTTCAACATTGAAGATTGATTTTGACACTCTCCCTCGTTTTTTCCCCTGTTGGTTGGTGTCCTTTGGTAGGTACTGGAGAATTTTCGAGGCAATGTTCAAACCAGATTAAGAAAACTAGATGAAGGGGTGGTCCAAGCAACACTATTGGCTCTTGCTGGACTTCGGCGTTTAAGTATGACAGAAAATGTTACTTCGATTCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATAGGGATTGCCTGTCGAACCGATGATGATAATATGGTATGTTTTCATCGTTTATGTAAACTAGTATTCACCCATGTTCGTCTGAAAATTGAAAAATGCCTTTATAACTACATCCAGTTGTGAAAACCATCAATTTGAGTCGAATCATTGACTTGTCTCCCCATACCATTAAAATTCATGGTTTGAAGAATAGAACTTTACCTTCTTTGAAGAACTTGACCTTGAGTTGAAGTTGGTCATTCCTGTTTGGTTTCTCATTTGCAGGCCAAATACTTAGCCTCATTGAACCACGAGGAAACAAGACTGGCTGTCGTTTGTGAGAGAGCATTCCTCGAGACACTCGATGGATCTTGCCGAACCCCAATAGCAGGATATGCTTCAAGAGATGAAGATGGAAACTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACCCGAGGTACGAACTGCATCCATGCTCAAATCATATAACTTTTGCTCTCATATGCATCAGCTTCTTCTCCATTGCTATACCATTGAATATCTTCATCTCTTAATCCTTACCTCCGCTTTGGTTCATTTTCAGTTCTTGAAACTTCTCGACGAGGTCTATACGCCCTCGATGATATGATTGCAATGGGAAAGGATGCTGGTAAGGAGCTTCTTTCTCGAGCAGGACCTGGGTTTTTTGATAGCTAGTTAGTTTAATTTAGAATGTAGGATAATGCCAGCGAGAAAAGATCAAGGCTTCATATCCAAATCTTGAAGTGAGTAGGAGTTCATATTCATCCTTTGAGATGGAGCTTGTTGTACTTTAAAGATTACTTTTTTTTCCACTTTGTTTTTCTTAATTGGTTGTGTAAATTAGAACAGAAATCATTTAGACTATCTTGGCTTCCCCAAATAATCAATACAATTTTCCCAAACATGAAGTACATAGGAAA

mRNA sequence

AAATGGATGAGAATCCTATCCGCCAACGCGAATGCAGACATTTGCCCACTGGGTCTCATCAATAACACAATCCCTTCCTTCTAAGCTAACCCTCTCTTCCCCATTTCCCCAATTCCCCCTTTTTTCTTCTAATTCCTCTCTCCAATGGCGGCTTTCTCTTCCCCTTCAATCAGCCACACTCCCATGGCTCGCCCCTGCAATGTTGGCTCCCTTTCCCTTCTTGGGTTCTCCTCTATTTCCCTTAAGCCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGGTTGGCCTCGTACGAGCCTCCGTTGCGGTGGAGCAGCAGGCAGAGAAGACCAAGGTCGCCCTCCTCAGAATTGGCACCAGAGGAAGCCCGTTAGCACTTGCCCAGGCTCACGAGACGAGAAACAAACTCATGGCTATCCATCCCGAGCTTGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTCGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGACGCACTTATTAATGGCGACATAGACATTGCCGTTCACTCTATGAAAGATGTTCCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCGAGGGAAGATGTTCGCGATGCGTTCATTTCTCTTAGTGCGGGGTCACTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTACTGGAGAATTTTCGAGGCAATGTTCAAACCAGATTAAGAAAACTAGATGAAGGGGTGGTCCAAGCAACACTATTGGCTCTTGCTGGACTTCGGCGTTTAAGTATGACAGAAAATGTTACTTCGATTCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATAGGGATTGCCTGTCGAACCGATGATGATAATATGGCCAAATACTTAGCCTCATTGAACCACGAGGAAACAAGACTGGCTGTCGTTTGTGAGAGAGCATTCCTCGAGACACTCGATGGATCTTGCCGAACCCCAATAGCAGGATATGCTTCAAGAGATGAAGATGGAAACTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCTATACGCCCTCGATGATATGATTGCAATGGGAAAGGATGCTGGTAAGGAGCTTCTTTCTCGAGCAGGACCTGGGTTTTTTGATAGCTAGTTAGTTTAATTTAGAATGTAGGATAATGCCAGCGAGAAAAGATCAAGGCTTCATATCCAAATCTTGAAGTGAGTAGGAGTTCATATTCATCCTTTGAGATGGAGCTTGTTGTACTTTAAAGATTACTTTTTTTTCCACTTTGTTTTTCTTAATTGGTTGTGTAAATTAGAACAGAAATCATTTAGACTATCTTGGCTTCCCCAAATAATCAATACAATTTTCCCAAACATGAAGTACATAGGAAA

Coding sequence (CDS)

ATGGCGGCTTTCTCTTCCCCTTCAATCAGCCACACTCCCATGGCTCGCCCCTGCAATGTTGGCTCCCTTTCCCTTCTTGGGTTCTCCTCTATTTCCCTTAAGCCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGGTTGGCCTCGTACGAGCCTCCGTTGCGGTGGAGCAGCAGGCAGAGAAGACCAAGGTCGCCCTCCTCAGAATTGGCACCAGAGGAAGCCCGTTAGCACTTGCCCAGGCTCACGAGACGAGAAACAAACTCATGGCTATCCATCCCGAGCTTGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTCGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGACGCACTTATTAATGGCGACATAGACATTGCCGTTCACTCTATGAAAGATGTTCCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCGAGGGAAGATGTTCGCGATGCGTTCATTTCTCTTAGTGCGGGGTCACTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTACTGGAGAATTTTCGAGGCAATGTTCAAACCAGATTAAGAAAACTAGATGAAGGGGTGGTCCAAGCAACACTATTGGCTCTTGCTGGACTTCGGCGTTTAAGTATGACAGAAAATGTTACTTCGATTCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATAGGGATTGCCTGTCGAACCGATGATGATAATATGGCCAAATACTTAGCCTCATTGAACCACGAGGAAACAAGACTGGCTGTCGTTTGTGAGAGAGCATTCCTCGAGACACTCGATGGATCTTGCCGAACCCCAATAGCAGGATATGCTTCAAGAGATGAAGATGGAAACTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCTATACGCCCTCGATGATATGATTGCAATGGGAAAGGATGCTGGTAAGGAGCTTCTTTCTCGAGCAGGACCTGGGTTTTTTGATAGCTAG

Protein sequence

MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQAEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALAGLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKDAGKELLSRAGPGFFDS
Homology
BLAST of Sed0006741 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.6 bits (1786), Expect = 1.9e-195
Identity = 355/376 (94.41%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS GAKKFHG GL+RASVAVEQQ
Sbjct: 18  MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQ 77

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 78  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 137

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 138 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 197

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 198 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 257

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 258 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 317

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 318 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 377

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 378 AGKELLSRAGPGFFDS 393

BLAST of Sed0006741 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 691.8 bits (1784), Expect = 3.3e-195
Identity = 353/376 (93.88%), Postives = 364/376 (96.81%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M   SSPS+SH+PM RP NVGSLSLLGFSS+SLKPPAFSNGAKKFHG GL+RASVAVEQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
            EKTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTENVTSILSIDEMLPAVAQGAIGIACRTDDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 691.0 bits (1782), Expect = 5.6e-195
Identity = 354/376 (94.15%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS G KKFHG GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. NCBI nr
Match: XP_022998456.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 690.6 bits (1781), Expect = 7.3e-195
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS G K+FHGVGL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. NCBI nr
Match: KAG7036601.1 (Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 353/376 (93.88%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS G KKFHG GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YL+SLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLSSLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.1e-156
Identity = 291/375 (77.60%), Postives = 322/375 (85.87%), Query Frame = 0

Query: 2   AAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQA 61
           ++FS PS    P        SL    F   S K   FS           +RAS+AVEQQ 
Sbjct: 9   SSFSLPSAPSNPSL------SLFTSSFRFSSFKTSPFSKCR--------IRASLAVEQQT 68

Query: 62  EKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLA 121
           ++ K AL+RIGTRGSPLALAQAHETR+KLMA H ELAE+GAIQIVIIKTTGDKILSQPLA
Sbjct: 69  QQNKTALIRIGTRGSPLALAQAHETRDKLMASHTELAEEGAIQIVIIKTTGDKILSQPLA 128

Query: 122 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 181
           DIGGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLPE+TILPCNLPREDVRDAFISLSA 
Sbjct: 129 DIGGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPEETILPCNLPREDVRDAFISLSAA 188

Query: 182 SLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALAG 241
           SLA+LPAGSVIGTASLRRKSQ+L+RYPSL V +NFRGNVQTRLRKL EGVV+ATLLALAG
Sbjct: 189 SLADLPAGSVIGTASLRRKSQILHRYPSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAG 248

Query: 242 LRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCERA 301
           L+RL+MTENVTS LSID+MLPAVAQGAIGIACR++DD MA+YLASLNHEETRLA+ CERA
Sbjct: 249 LKRLNMTENVTSTLSIDDMLPAVAQGAIGIACRSNDDKMAEYLASLNHEETRLAISCERA 308

Query: 302 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKDA 361
           FL TLDGSCRTPIAGYASRD+DGNC+F+GLVASPDGTRVLETSR G Y  +DM+ +GKDA
Sbjct: 309 FLTTLDGSCRTPIAGYASRDKDGNCLFRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDA 368

Query: 362 GKELLSRAGPGFFDS 377
           G+ELLSRAGPGFF+S
Sbjct: 369 GEELLSRAGPGFFNS 369

BLAST of Sed0006741 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.4e-156
Identity = 282/357 (78.99%), Postives = 313/357 (87.68%), Query Frame = 0

Query: 18  CNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQAEKTKVALLRIGTRGSP 77
           C++GS+S +GFS   +  PA     +K    G V+A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 78  LALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDAL 137
           LALAQA+ETR KL   HPEL EDGAI I IIKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 138 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASL 197
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGSV+GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 198 RRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALAGLRRLSMTENVTSILSI 257
           RRKSQ+L++YP+L+V ENFRGNVQTRL KL  G VQATLLALAGL+RLSMTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 258 DEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 317
           DEMLPAVAQGAIGIACRTDDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 318 ASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKDAGKELLSRAGPGFF 375
           AS+DE+GNCIF+GLVASPDGT+VLETSR+G Y  +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of Sed0006741 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 8.5e-138
Identity = 250/326 (76.69%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 51  VRASVAVEQQAEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKT 110
           VRA+VAV+ +A+  KV+L+RIGTRGSPLALAQAHETR+KL A H ELAE+GA++IVIIKT
Sbjct: 33  VRAAVAVQAEAQ-AKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 111 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 170
           TGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 171 VRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEG 230
           VRDAFI L+A SLAELPAGSV+G+ASLRR+SQ+L +YPSL V+ NFRGNVQTRLRKL EG
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 231 VVQATLLALAGLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHE 290
            V ATLLALAGL+RL+M E  TS+LS+DEMLPAVAQGAIGIACR+ DD M  YL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 291 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYA 350
           +TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR G Y 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 351 LDDMIAMGKDAGKELLSRAGPGFFDS 377
            D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

BLAST of Sed0006741 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.6e-83
Identity = 171/307 (55.70%), Postives = 216/307 (70.36%), Query Frame = 0

Query: 69  LRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGL 128
           +RIGTRGS LALAQAHETR++LMA H  LA D A +IV+IKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 129 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPA 188
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 189 GSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALAGLRRLSMT 248
           G+V+G++SLRR++QL  R P L ++E FRGNVQTRL+KL++GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 249 ENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCERAFLETLDG 308
                 +  DEMLPAVAQG IG+  R DD   A  LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 309 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKDAGKELLSR 368
           SC+TPIAG A    D     +G +  PDG+ V+   R G  A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 369 AGPGFFD 376
           A   FFD
Sbjct: 311 APADFFD 311

BLAST of Sed0006741 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-83
Identity = 173/316 (54.75%), Postives = 213/316 (67.41%), Query Frame = 0

Query: 59  QQAEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 118
           Q +++T + L  IGTRGSPLALAQAHE R++L   H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 119 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 178
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 179 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLA 238
            AGSL +LPAG+ +GTASLRR++ +L   P L V  + RGNV+TRLRK+  G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 239 LAGLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVC 298
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 299 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMG 358
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 359 KDAGKELLSRAGPGFF 375
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

BLAST of Sed0006741 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.7e-195
Identity = 354/376 (94.15%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS G KKFHG GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.5e-195
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSPSISH PMARPCN+GSLS  GFSS+SLKPPAFS G K+FHGVGL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           AEKTKVAL+RIGTRGSPLALAQAHETR KLMAIHPELAEDGAIQIVIIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTE VTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.6e-193
Identity = 347/376 (92.29%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M A SSPS+SH+PMA PCN+ SLSL GFSS+SLKPPAF+NG KK HG GL+RASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
           A KTKVALLRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTENVTSILSIDEMLPAVAQGAIGIACR+DDD MA+YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.2e-192
Identity = 347/376 (92.29%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M A SSPS+S++PMARPCN+ SLSL GFSS+SLKPPAF+N  KKFHG GL+RASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
            EKTKVALLRIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTENVTSILSIDEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YA+DDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.7e-192
Identity = 346/376 (92.02%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAAFSSPSISHTPMARPCNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQ 60
           M  FSSP I+H+PMARPCNVGSLS LGFSS+SLKPPAF NG KKF G+GLVRASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  AEKTKVALLRIGTRGSPLALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120
            EKTKVAL+RIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKL+EGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLSMTENVTSILSIDEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCER 300
           GLRRL+MTENVTS+LSIDEMLPAVAQGAIGIACR++DD MA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKD 360
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YA+DDM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

BLAST of Sed0006741 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-157
Identity = 282/357 (78.99%), Postives = 313/357 (87.68%), Query Frame = 0

Query: 18  CNVGSLSLLGFSSISLKPPAFSNGAKKFHGVGLVRASVAVEQQAEKTKVALLRIGTRGSP 77
           C++GS+S +GFS   +  PA     +K    G V+A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 78  LALAQAHETRNKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDAL 137
           LALAQA+ETR KL   HPEL EDGAI I IIKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 138 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASL 197
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGSV+GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 198 RRKSQLLNRYPSLNVLENFRGNVQTRLRKLDEGVVQATLLALAGLRRLSMTENVTSILSI 257
           RRKSQ+L++YP+L+V ENFRGNVQTRL KL  G VQATLLALAGL+RLSMTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 258 DEMLPAVAQGAIGIACRTDDDNMAKYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 317
           DEMLPAVAQGAIGIACRTDDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 318 ASRDEDGNCIFKGLVASPDGTRVLETSRRGLYALDDMIAMGKDAGKELLSRAGPGFF 375
           AS+DE+GNCIF+GLVASPDGT+VLETSR+G Y  +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023523547.11.9e-19594.41porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_038906286.13.3e-19593.88porphobilinogen deaminase, chloroplastic [Benincasa hispida][more]
XP_022949290.15.6e-19594.15porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata][more]
XP_022998456.17.3e-19593.88porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima][more]
KAG7036601.11.2e-19493.88Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
Q430821.1e-15677.60Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S... [more]
Q433162.4e-15678.99Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC... [more]
Q6H6D28.5e-13876.69Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994... [more]
A1B8531.6e-8355.70Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586... [more]
Q3SVF32.1e-8354.75Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1... [more]
Match NameE-valueIdentityDescription
A0A6J1GCC72.7e-19594.15Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ... [more]
A0A6J1KA973.5e-19593.88Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV... [more]
A0A6J1F3549.6e-19392.29Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ... [more]
A0A6J1IZW21.2e-19292.29Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV... [more]
A0A6J1DII94.7e-19292.02Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G08280.11.7e-15778.99hydroxymethylbilane synthase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000860Porphobilinogen deaminasePRINTSPR00151PORPHBDMNASEcoord: 211..228
score: 56.88
coord: 191..208
score: 56.08
coord: 112..132
score: 66.21
coord: 143..162
score: 61.19
coord: 298..315
score: 53.17
IPR000860Porphobilinogen deaminaseTIGRFAMTIGR00212TIGR00212coord: 69..367
e-value: 4.0E-101
score: 335.9
IPR000860Porphobilinogen deaminasePIRSFPIRSF001438PBGDcoord: 50..376
e-value: 4.2E-117
score: 388.6
IPR000860Porphobilinogen deaminasePANTHERPTHR11557PORPHOBILINOGEN DEAMINASEcoord: 15..375
IPR000860Porphobilinogen deaminaseHAMAPMF_00260Porphobil_deamcoord: 68..368
score: 34.328724
NoneNo IPR availableGENE3D3.40.190.10coord: 69..278
e-value: 5.8E-82
score: 276.0
NoneNo IPR availableGENE3D3.40.190.10coord: 170..270
e-value: 5.8E-82
score: 276.0
NoneNo IPR availablePANTHERPTHR11557:SF8BNAC02G01240D PROTEINcoord: 15..375
NoneNo IPR availableCDDcd13648PBP2_PBGD_1coord: 68..347
e-value: 0.0
score: 509.646
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 69..286
IPR022418Porphobilinogen deaminase, C-terminalPFAMPF03900Porphobil_deamCcoord: 294..366
e-value: 3.8E-15
score: 56.0
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilyGENE3D3.30.160.40coord: 289..375
e-value: 2.2E-23
score: 84.4
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilySUPERFAMILY54782Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domaincoord: 288..375
IPR022417Porphobilinogen deaminase, N-terminalPFAMPF01379Porphobil_deamcoord: 69..281
e-value: 3.7E-76
score: 255.2
IPR022419Porphobilinogen deaminase, dipyrromethane cofactor binding sitePROSITEPS00533PORPHOBILINOGEN_DEAMcoord: 299..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0006741.1Sed0006741.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018160 peptidyl-pyrromethane cofactor linkage
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0017157 regulation of exocytosis
biological_process GO:0033014 tetrapyrrole biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0004418 hydroxymethylbilane synthase activity
molecular_function GO:0045159 myosin II binding
molecular_function GO:0019905 syntaxin binding