Sed0006385 (gene) Chayote v1

Overview


Name	Sed0006385
Type	gene
Organism	Sechium edule (Chayote v1)
Description	3-hydroxy-3-methylglutarate-CoA lyase
Location	LG05: 17985043 .. 17990393 (+)
RNA-Seq Expression	Sed0006385
Synteny	Sed0006385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAGAGAAAGAAGAGGGGAATGGAAGGGAAGGAAGAAATCACCAACTTCTAACTGACAACTACCCACCACTTCCCCTTCTCCAGAAAAAGAAGACCCAATATTTATTCATACTGACTTTAGGCATTAACGACGTCCACCAACCCAACAACACAGGGAAATTTCAAGCTCGATTTCTCTATATAATATTTCTTCTCATCGGTTGAAATTTCCCTGTCTCTGCACCGCCAAGTTGTTCTTTCCTTAATTTCCACTTCAGGTTCCATTCTTTTCTTCTCTTAATCCTTTTTCACCCTTTCCCCCCTTTGTTTTTTCTTCTGGGTTTTCTGTTGCTTCTGTTTGGCTGCCCAGAAACTGCAGGTATACAGAATCCACATATTCTAGAATTCATTCTTGTTTTTTTTTTCTTTTTTAAAATTGAATTATGAATATGATCGCTGCCCCAAAATTTCGATCGATTCTGTTACTTCTTTCTTTTATGCGCTCTACTTGTTAATTTAATGGAAGTTTGATGAAATTGATTTATAATTCTGAATTTTTGTTTTCTTATTTCCAACTCCGAGCCCTTGTTTTTGGGGTTTAATGCACTCATCGTTTTGTCCGCCCCTTTATTCTTCATAATTAATTTGATTATGGCAGAGTCATCGTCTATGATTCACATTCTCTTTATTGGGTCGAAGTCGATAATCAACGGCCCATCGATGGAAACTCTCATCTTGAGATGTCCTTGTAATGGTGTAATTCAATATGCATATATAATGTGCAGTGAAATGGGCATATGATAGGTGTTGGTATTTTTGTTCTTCATTGATGGTATCCGAGATCAGAACAAGCATGCTCGCGCGGCCTTTTGTCGTCCCATTCTTGAGTGAGTTCAGTGTTGAAATACCTATGGAATAGATGCTAGAGTAGATTGGTACTTCTCTGAGTATTGGGAGGAATGTGCCCTTTTCCTTTTAAGAATCTAAGATTGGTTTGCAATGGAGGATTTATGAAAACTAGATAAGTAGAGCTCATTTTGGGATTTGTTGCTTTACATCATCTCTATACTATTCAATGTGATGGACAGTACTTAAACGGGATACAAAATTACTAGACATTATTGTTGTAACTAACTATGAAGGGTAAGATCAATGGATATTTCAATCGAAACGAAAGCACACCAGTTGATTGAAGCGATTATAAAACTAGATTTGACCAATCTATTATTAAGGTTTCATCTGTTATTGATTTTCTGCAAGTGAGAAAGCTATACCACCCTAGACAAGGCAGGAATCTTATTTGGTTTACTTCTCAAACTCAGTTGTGGTTTTTTCTTCGGCCTCCACAATGTCAAGTTTGGAGGAGCCACTTGGTCTTGAAAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTCTCTTCTGGTGCGTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTACTGGATTGAAGGGTGGAGAAGTAGCTCATCCAATATTTGCAAGTAAGCAGAATATTGATCTTCCTTCACTTGGTCTTATTTTTCAGTTTCAGTTTTCGTTTGCATTTTGTGCTTGTGCGATTCTTGTTGATATGGGCTGGTTTATGACAGAAGACCATGCAAAGTATCCAGGCATATTGATCTTTATCAGTTATTTCTCACTAGATTGTCATTTTTTGTAATCACTCTGGCACACTTTTCCCCAACGTGACATGAATTGACCTGTGCTTCTTCTTATTTTTTTAAACTGTACCGTTGTGTGTGTGTGTTTTTTTTCCTTTCAACAGGGAATATGATGAAGATGCTAGGGATACATTTCCGTGGAGAAGAATTAGGAAGGACACATCCCAAAAGGCCTCTGTCAATAGGAGGACAATGAGCGTAGGCTCGAAAAATTTTGTATCGGGAGATTTTTGTGATTCACATTGTTCTCCAAGTCATGAGTACCAGAATAAGAGCAGTAGCGGCAATACACAACATATGGGGAATAAGGTGATTTAATAACTATTAGACCCGTGCATATGCAATTACTCCCTTTGAGATTTTTTTTTTAATAGGAAATGTTACAAAAGAGATCCTACCAATAATTGCAAAAACTACTCCAGTGCGCAAATAGAGCAGTAAAGCTATCATTCTTGAAAGGTGTAATAGTATAAGCCAATGTCATAGCAAGAAAAGTAATGGATTAAGATTTTTTTTTTTGGTGCACCATTGGAGAACATAATGTCATTTCTAGCCTTCCGATCGACCACCAAAATGCGCTGAGAAATAAGCACCATAAGATTCTCTTTTCATCTTTAAAGGGTGTTGACAGTAGGATTGTATGATCAGACCTGGATGTTGCCTGGTTTCACCATTTGTTCACAACTAATAGAGAGGCGTTTCCCTCCAATTACATTTGTTTTATCTGCCAGCATTCATACAGTTCTATCTCCTTTTACTTGTTTTTCTTGCTCTACCTAGTACCTATCATATCATGCACTTTGTGAAGTTTCATCAGATGATTTGAAGGTTTATAGAATTTCTCTCTTAGAATTTGACCTTTTTTTTCTGATCTTATTTTGAATTTGGGCCTCCAGGTTTTGAATGGCATGCCAAAGTTTGTGAAGATAGTTGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACATTTGTCAAGGTCGAATTGATCCATAGACTAGTATCTTCAGGCTTGCCCGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGGTAATTTCTGTACAAGTTCCCTTTCACCTCTTGTAGACAAATTTCTAAATGCTATTTTTTTAAAAAAAAGTTTGAAAACTTTCTTGTTTCTTTCGAGCTGAATGTTTAGTAAGAGACGAAAAAAATTCAGCATACCAGAGCACTCTTTGTCTTAAAATGGCTGGTGGCATTTGTTAGCTTCTGTGACTTCTGTTATGTCTACCTAGTAGTTTCGTCTTTACCGTGTATCGTGCTTGATTAATTTTGAAATGAACTGATGTTGCTGTGGGTTTAAAAAGTTATTCTTGAAACTTCTTTTCATTTGGGTAAGTTATGATATGAACTGATTAGGAAATAGCGTTCTTGAAGCTTTTCTCTCCATAGTTGATCAAATGAAAATTTAAATTCATCATGAATTTCTTTGATTTAGCATACTTTGGTATCACTAGGGCTCAAATATAAAAGGTATGCATATATTTTTGTTCTCCCTTTGGTCCATGCAGTCCTGTTCCAAGACTTTCTTTCCATGTAACCAATTTATGAGAACTTGAATGCTCACTCCTGACTCTCCTGACTTATGAGCTTCATTTAAATTTTAAATTTCTCATTGGCACACCCTTTGTCCAGTTATCTGATGCAGCTCAAGTGATGGAAGCTGTTTGTAATCTGGAAGGTGTCAGATTGCCAGTTTTAACTCCTAATATGAAAGTGGGTCATCTTCTAAAAAAAAGTGGAATTATTTTTCAAACATTGACAGATTGAATCCTCTATTTCTAAAGCCCCACCTTCTTCCAGGGCTTTCAAGCTGCTATTGCGGCTGGAGCAAAGGAAGTAGCAGTCTTTGCATCAGCCTCTGAATCATTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCTGTAACTTCTGCTGCAAAAGAGCTTGGAATCCCTGTTCGAGGGTATGACATTAATCTGCATTCAAGATGAGAGAAAAAGAACTTTATTAATTCAGGCACTGTCATTATGGCTTTAACATGACCAGGCTTATCTTCAACCATGAGTGACGGTTTCGAAAATTTGTTGCCACGTTAAATAAAGAACAACAAGTAGACATGTCTTATCATCTTTCAATGATATAGTCTGGAAATATAGACCATGTACTTTCTGAATAGTAACCGATGAATATTTCCTTTGGAATTTGGTTTCATATTTTCAATAGGATATATTTTCTGGTATATCGTTCTCCACATTGTGCTCTTGGAGGGTTCTCTAACTGATATAAGTGGCTATGGATTTGTATAGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAATTCCACCATCTAAAGTGGCTTATGTAGCAAAAGAGCTTTATGAGATGGGTTGCTTCGAAATTTCTCTAGGGGACACCATTGGAGTTGCCACGCCTGGTAATAATTTAAACCTTTCTATTTTTCCTCTATTTTTTTAATATCTATGAATGTCTGGGCCACCTAAGTCAACTGTTAGATACTATTTTAAAGTTTCTTATTTGTTTTTATTTTTATTTTAAAAATGAGAAATATATGATTTAGCAATCAATTTTTGTTTTTGAAATGTATTATCAATTTCAAACAAAAAGCTGAAAACAAAATTGATGTAAACAAAAATAGAAAACGGAAATGATAATTGATTCACTAGAATCCTACAGTGAGTTGCCTTTTGTACTCAACATGAATTATACAACCATATGAGTTTCTTGAAACATATGAGTTGCCTTTTATCTTATCTTATCTTCACTTCTCTTTCTGTTGGCACAGGGACTGTTGTTCCCATGCTCGAAGTCGTAATGGCTGCCGTACCTGTCGACAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAGTAAGGCCTCTTTTGAAGCTGAGTTATATCCTCAAAACTTGCTTTCCAGATATAGGGATCCAAATTACTTCACAAGCTTGCCATAGTTTAGCCAACTTTAACCATTAACTGATGTTTTGATGTTTTGTTGAACAGATGGGGATTAGCACAATTGATTCCTCTGTTGCAGGTTTGGGCGGATGCCCATATGCAAAAGGAGCTTCAGGGAATGTTGCAACTGAGGATGTGGTGTATATGCTCCATGGACTTGGTATAAAAACTAATGTAGATTTGGAAAAGGTCATGTCTGCTGGGGATTTCATCAACAACCATTTGGGTCGGCCTTCGGGTTCGAAAACCGCCATTGCATTGAGCAATGTTGCAGTTGATTCTTCCATGATGTAACACATCTTGGTCAGACACACTTCAAGAAACTATCTTATCTTATATATGTTGTCTTATCTGGCTGGAATATGATGCAGCCATATACATATATACCAGAACTCAAATAACATGATTATCATCAAACATTTAGTAGAAAATTTATGGGTCTGAATTCCCTCCCTAATTTGAAAAGTTAGTCGATTCTTTGTGTCAGTGGTTTGAAAGGTTGAGCTATTATCATGTCATAGTTCATTGGGTATAAGATATATATGTAATGTCAAAAAAGATTAAGTCAGAGATGAAATTCTGATTACTATTTTTCTTGCCCTGAG

mRNA sequence

AAAAAAGAGAAAGAAGAGGGGAATGGAAGGGAAGGAAGAAATCACCAACTTCTAACTGACAACTACCCACCACTTCCCCTTCTCCAGAAAAAGAAGACCCAATATTTATTCATACTGACTTTAGGCATTAACGACGTCCACCAACCCAACAACACAGGGAAATTTCAAGCTCGATTTCTCTATATAATATTTCTTCTCATCGGTTGAAATTTCCCTGTCTCTGCACCGCCAAGTTGTTCTTTCCTTAATTTCCACTTCAGTTGTGGTTTTTTCTTCGGCCTCCACAATGTCAAGTTTGGAGGAGCCACTTGGTCTTGAAAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTCTCTTCTGGTGCGTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTACTGGATTGAAGGGTGGAGAAGTAGCTCATCCAATATTTGCAAGGAATATGATGAAGATGCTAGGGATACATTTCCGTGGAGAAGAATTAGGAAGGACACATCCCAAAAGGCCTCTGTCAATAGGAGGACAATGAGCGTAGGCTCGAAAAATTTTGTATCGGGAGATTTTTGTGATTCACATTGTTCTCCAAGTCATGAGTACCAGAATAAGAGCAGTAGCGGCAATACACAACATATGGGGAATAAGGTTTTGAATGGCATGCCAAAGTTTGTGAAGATAGTTGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACATTTGTCAAGGTCGAATTGATCCATAGACTAGTATCTTCAGGCTTGCCCGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAGCTCAAGTGATGGAAGCTGTTTGTAATCTGGAAGGTGTCAGATTGCCAGTTTTAACTCCTAATATGAAAGGCTTTCAAGCTGCTATTGCGGCTGGAGCAAAGGAAGTAGCAGTCTTTGCATCAGCCTCTGAATCATTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCTGTAACTTCTGCTGCAAAAGAGCTTGGAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAATTCCACCATCTAAAGTGGCTTATGTAGCAAAAGAGCTTTATGAGATGGGTTGCTTCGAAATTTCTCTAGGGGACACCATTGGAGTTGCCACGCCTGGGACTGTTGTTCCCATGCTCGAAGTCGTAATGGCTGCCGTACCTGTCGACAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGGATTAGCACAATTGATTCCTCTGTTGCAGGTTTGGGCGGATGCCCATATGCAAAAGGAGCTTCAGGGAATGTTGCAACTGAGGATGTGGTGTATATGCTCCATGGACTTGGTATAAAAACTAATGTAGATTTGGAAAAGGTCATGTCTGCTGGGGATTTCATCAACAACCATTTGGGTCGGCCTTCGGGTTCGAAAACCGCCATTGCATTGAGCAATGTTGCAGTTGATTCTTCCATGATGTAACACATCTTGGTCAGACACACTTCAAGAAACTATCTTATCTTATATATGTTGTCTTATCTGGCTGGAATATGATGCAGCCATATACATATATACCAGAACTCAAATAACATGATTATCATCAAACATTTAGTAGAAAATTTATGGGTCTGAATTCCCTCCCTAATTTGAAAAGTTAGTCGATTCTTTGTGTCAGTGGTTTGAAAGGTTGAGCTATTATCATGTCATAGTTCATTGGGTATAAGATATATATGTAATGTCAAAAAAGATTAAGTCAGAGATGAAATTCTGATTACTATTTTTCTTGCCCTGAG

Coding sequence (CDS)

ATGTCAAGTTTGGAGGAGCCACTTGGTCTTGAAAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTCTCTTCTGGTGCGTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTACTGGATTGAAGGGTGGAGAAGTAGCTCATCCAATATTTGCAAGGAATATGATGAAGATGCTAGGGATACATTTCCGTGGAGAAGAATTAGGAAGGACACATCCCAAAAGGCCTCTGTCAATAGGAGGACAATGAGCGTAGGCTCGAAAAATTTTGTATCGGGAGATTTTTGTGATTCACATTGTTCTCCAAGTCATGAGTACCAGAATAAGAGCAGTAGCGGCAATACACAACATATGGGGAATAAGGTTTTGAATGGCATGCCAAAGTTTGTGAAGATAGTTGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACATTTGTCAAGGTCGAATTGATCCATAGACTAGTATCTTCAGGCTTGCCCGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAGCTCAAGTGATGGAAGCTGTTTGTAATCTGGAAGGTGTCAGATTGCCAGTTTTAACTCCTAATATGAAAGGCTTTCAAGCTGCTATTGCGGCTGGAGCAAAGGAAGTAGCAGTCTTTGCATCAGCCTCTGAATCATTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCTGTAACTTCTGCTGCAAAAGAGCTTGGAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAATTCCACCATCTAAAGTGGCTTATGTAGCAAAAGAGCTTTATGAGATGGGTTGCTTCGAAATTTCTCTAGGGGACACCATTGGAGTTGCCACGCCTGGGACTGTTGTTCCCATGCTCGAAGTCGTAATGGCTGCCGTACCTGTCGACAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGGATTAGCACAATTGATTCCTCTGTTGCAGGTTTGGGCGGATGCCCATATGCAAAAGGAGCTTCAGGGAATGTTGCAACTGAGGATGTGGTGTATATGCTCCATGGACTTGGTATAAAAACTAATGTAGATTTGGAAAAGGTCATGTCTGCTGGGGATTTCATCAACAACCATTTGGGTCGGCCTTCGGGTTCGAAAACCGCCATTGCATTGAGCAATGTTGCAGTTGATTCTTCCATGATGTAA

Protein sequence

MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDEDARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQHMGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNINCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEISLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAIALSNVAVDSSMM

Homology

[Close]

BLAST of Sed0006385 vs. NCBI nr
Match: XP_022985653.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 756.5 bits (1952), Expect = 1.2e-214
Identity = 381/432 (88.19%), Postives = 400/432 (92.59%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPLGL+KLPSMSTIDR+QRFSSGACRPIGDDMGMGI WI+G RSSSSN CKEYDE
Sbjct: 22  MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 81

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKDTSQ  SVNRRTMS G +  VSG+FCDSHCSP HEY+ KSSS N QH
Sbjct: 82  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 141

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
             NK L  +PKFVKIVEVGPRDGLQNEK MVPTFVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 142 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 201

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDAA+VMEAV NLEG RLPVLTPN+KGF+AA+ AGAKEVAVFASASESFSKSNIN
Sbjct: 202 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 261

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEE LDRYRAVTSAAKEL IPVRGYVSCVVGCPTEG+IPPSKVAYVAKELYEMGCFEI
Sbjct: 262 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 321

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 322 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDL KVMSAG+FI+NHLGRPSGSKT+I
Sbjct: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 441

Query: 421 ALSNVAVDSSMM 433
           A + +A D+S M
Sbjct: 442 AFNQLAADASKM 453

[Close]

BLAST of Sed0006385 vs. NCBI nr
Match: XP_022985654.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP_022985655.1 hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP_022985656.1 hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 756.5 bits (1952), Expect = 1.2e-214
Identity = 381/432 (88.19%), Postives = 400/432 (92.59%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPLGL+KLPSMSTIDR+QRFSSGACRPIGDDMGMGI WI+G RSSSSN CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKDTSQ  SVNRRTMS G +  VSG+FCDSHCSP HEY+ KSSS N QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 120

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
             NK L  +PKFVKIVEVGPRDGLQNEK MVPTFVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDAA+VMEAV NLEG RLPVLTPN+KGF+AA+ AGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEE LDRYRAVTSAAKEL IPVRGYVSCVVGCPTEG+IPPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDL KVMSAG+FI+NHLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALSNVAVDSSMM 433
           A + +A D+S M
Sbjct: 421 AFNQLAADASKM 432

[Close]

BLAST of Sed0006385 vs. NCBI nr
Match: XP_023512797.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023512798.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 754.6 bits (1947), Expect = 4.7e-214
Identity = 381/432 (88.19%), Postives = 400/432 (92.59%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPLGL+KLPSMSTIDR+QRFSSGACRPIGDDMGMGI WI+G RSSSSN CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKDTSQ  SVNRRTMS G +  VSG+FCDSHCSP HEY+ KSSS N QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
             NK L  +PKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDAA+VMEAV NLEG RLPVLTPN+KGF+AA+AAGAKEVAVFASASE FSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKEL IPVRGYVSCVVGCPTEG+IPPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDL KVMSAG+FI+NHLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALSNVAVDSSMM 433
           A + +A D+S M
Sbjct: 421 AFNQLAADASKM 432

[Close]

BLAST of Sed0006385 vs. NCBI nr
Match: XP_022995070.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 753.1 bits (1943), Expect = 1.4e-213
Identity = 381/430 (88.60%), Postives = 402/430 (93.49%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSS+EEPLGL+KLPSMSTIDRIQRFSSGACRPIGDD+GMGI WIEG RSS SN C+EYDE
Sbjct: 60  MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGMGICWIEGGRSSLSNSCREYDE 119

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKD SQK SVN+RTMS+GSK FV+GDFCDSHCS S EYQ +SSS + Q+
Sbjct: 120 DARETFPWRRHRKDASQKGSVNKRTMSIGSKKFVTGDFCDSHCSSSPEYQTESSSHHIQN 179

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
           M NK  NG+PKFVKIVEVGPRDGLQNEKDMVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 180 MANKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 239

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME V NLEGVRLPVLTPNMKGF+AA+AAGAKEVAVFASASESFSKSNIN
Sbjct: 240 RVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAAGAKEVAVFASASESFSKSNIN 299

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPP KVAYVAK+LY+MGCFEI
Sbjct: 300 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPYKVAYVAKQLYDMGCFEI 359

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 360 SLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 419

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDL KV+SAG+FINNHLGRPSGSKTAI
Sbjct: 420 AGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKVISAGNFINNHLGRPSGSKTAI 479

Query: 421 ALSNVAVDSS 431
           AL  VA D+S
Sbjct: 480 ALGRVAADAS 489

[Close]

BLAST of Sed0006385 vs. NCBI nr
Match: XP_022995072.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X3 [Cucurbita maxima] >XP_022995073.1 hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 753.1 bits (1943), Expect = 1.4e-213
Identity = 381/430 (88.60%), Postives = 402/430 (93.49%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSS+EEPLGL+KLPSMSTIDRIQRFSSGACRPIGDD+GMGI WIEG RSS SN C+EYDE
Sbjct: 1   MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGMGICWIEGGRSSLSNSCREYDE 60

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKD SQK SVN+RTMS+GSK FV+GDFCDSHCS S EYQ +SSS + Q+
Sbjct: 61  DARETFPWRRHRKDASQKGSVNKRTMSIGSKKFVTGDFCDSHCSSSPEYQTESSSHHIQN 120

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
           M NK  NG+PKFVKIVEVGPRDGLQNEKDMVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MANKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME V NLEGVRLPVLTPNMKGF+AA+AAGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPP KVAYVAK+LY+MGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPYKVAYVAKQLYDMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDL KV+SAG+FINNHLGRPSGSKTAI
Sbjct: 361 AGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKVISAGNFINNHLGRPSGSKTAI 420

Query: 421 ALSNVAVDSS 431
           AL  VA D+S
Sbjct: 421 ALGRVAADAS 430

[Close]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 581.6 bits (1498), Expect = 7.2e-165
Identity = 300/432 (69.44%), Postives = 350/432 (81.02%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPL  +KLPSMST+DRIQRFSSGACRP  DD+GMG  WIEG   ++SN C + D+
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 95

Query: 61  D-ARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQ 120
             A+++FPWRR  +  S+   + R     G  + VSG   +S      +Y   S+   T 
Sbjct: 96  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 155

Query: 121 HMGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSP 180
           H+ NK+  G+PKFVKIVEVGPRDGLQNEK++VPT VKVELI RLVSSGLPVVEATSFVSP
Sbjct: 156 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 215

Query: 181 TRVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNI 240
             VPQL+DA  VM+AV  L+G RLPVLTPN+KGFQAA++AGAKEVA+FASASESFS SNI
Sbjct: 216 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 275

Query: 241 NCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE  +PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 276 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 335

Query: 301 ISLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIG+ TPG+VVPMLE VMA VP DKLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 336 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 395

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTA 420
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AGDFI+ HLGRP+GSK A
Sbjct: 396 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 455

Query: 421 IALS-NVAVDSS 431
           +AL+  +  D+S
Sbjct: 456 VALNRRITADAS 466

[Close]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.8e-106
Identity = 193/292 (66.10%), Postives = 235/292 (80.48%), Query Frame = 0


Query: 130 PKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAA 189
           P+ VK+VEVGPRDGLQNEK +VPT VK+ LI  L  +GLPV+EATSFVSP  VPQ++D A
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 190 QVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNINCSIEESLDR 249
           +VM+ +  L GV  PVLTPN+KGFQAA+AAGAKEV++F +ASE F+K NINCSIEESL+R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 250 YRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEISLGDTIGVA 309
           +  V +AA+   IPVRGYVSCV+GCP EG I  +KVA V+K++Y MGC+EISLGD IG+ 
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 310 TPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 369
           TPG++  ML  VM  VPV  LAVH HDTYGQ+L NILV+LQMG+S +D+SVAGLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 370 KGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAIA 422
           +GASGNVATED+VYML+GLGI T VDL+K+M  G FI N L R + SK + A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

[Close]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot
Match: A8WG57 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgcll1 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 7.5e-106
Identity = 192/296 (64.86%), Postives = 235/296 (79.39%), Query Frame = 0


Query: 129 MPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDA 188
           +P +VKIVEVGPRDGLQNEK++VPT VK++LI  L  +GLPV+EATSFVS   V Q++D 
Sbjct: 39  LPVYVKIVEVGPRDGLQNEKEIVPTEVKIQLIDLLSQTGLPVIEATSFVSSKWVAQMADH 98

Query: 189 AQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNINCSIEESLD 248
             V++ +     VR PVLTPN++GFQAA+AAGA EVAVF SASE+FS+ NINCSIEESL 
Sbjct: 99  TAVLKGIKRSPDVRYPVLTPNIQGFQAAVAAGANEVAVFGSASETFSRKNINCSIEESLQ 158

Query: 249 RYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEISLGDTIGV 308
           R+  V SAAK+ GIPVRGYVSC +GCP EG + PS+V  VAK L+E+GC+E+SLGDTIGV
Sbjct: 159 RFEQVVSAAKQEGIPVRGYVSCALGCPYEGQVKPSQVTKVAKRLFELGCYEVSLGDTIGV 218

Query: 309 ATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPY 368
            T G++  ML  V+  VP   LAVH HDTYGQ+LPNIL++LQMG+S +D+SVAGLGGCP+
Sbjct: 219 GTAGSMAEMLSDVLTEVPAGALAVHCHDTYGQALPNILIALQMGVSVVDASVAGLGGCPF 278

Query: 369 AKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAIALSN 425
           AKGASGNV+TED++YMLHGLGI+T VDL KVM AGDFI   L R + SK + A  N
Sbjct: 279 AKGASGNVSTEDLLYMLHGLGIETGVDLLKVMEAGDFICKALNRKTNSKVSQATRN 334

[Close]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot
Match: Q8JZS7 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.9e-105
Identity = 190/303 (62.71%), Postives = 239/303 (78.88%), Query Frame = 0


Query: 126 LNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPTRVPQL 185
           L G+P++VKIVEVGPRDGLQNEK +VPT +K+ELI++L  +GL V+E TSFVS   VPQ+
Sbjct: 41  LPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQM 100

Query: 186 SDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNINCSIEE 245
           +D A+VM  +    GVR PVLTPN++GFQ A+AAGA E+AVF +ASESFSK NINCSIEE
Sbjct: 101 ADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEE 160

Query: 246 SLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEISLGDT 305
           S+ R++ V S+A+ + IPVRGYVSC +GCP EG+I P KV  V+K LY MGC+EISLGDT
Sbjct: 161 SMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDT 220

Query: 306 IGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGG 365
           IGV TPG++  MLE VM  +P   LAVH HDTYGQ+L NIL +LQMGI+ +DS+V+GLGG
Sbjct: 221 IGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGG 280

Query: 366 CPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAIALSNV 425
           CPYAKGASGNVATED++YML+G+G+ T VDL KVM AG+FI   + + + SK A A  N 
Sbjct: 281 CPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQASFNA 340

Query: 426 AVD 429
            ++
Sbjct: 341 RLE 343

[Close]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot
Match: Q29448 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Bos taurus OX=9913 GN=HMGCL PE=2 SV=2)

HSP 1 Score: 379.8 bits (974), Expect = 4.1e-104
Identity = 193/292 (66.10%), Postives = 230/292 (78.77%), Query Frame = 0


Query: 130 PKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAA 189
           PK VKIVEVGPRDGLQNEK++VPT VK++LI  L  +GLPVVEATSFVSP  VPQ++D A
Sbjct: 30  PKQVKIVEVGPRDGLQNEKNIVPTPVKIKLIDMLSEAGLPVVEATSFVSPKWVPQMADHA 89

Query: 190 QVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNINCSIEESLDR 249
           +V++ +    GV  PVLTPN KGFQAA+AAGAKEVA+F +ASE F+K NINCSI+ESL R
Sbjct: 90  EVLKGIQKFPGVNYPVLTPNFKGFQAAVAAGAKEVAIFGAASELFTKKNINCSIDESLQR 149

Query: 250 YRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEISLGDTIGVA 309
           +  +  AA+  GI VRGYVSCV+GCP EG I P+KVA V K+LY MGC+EISLGDTIGV 
Sbjct: 150 FDEILKAARAAGISVRGYVSCVLGCPYEGKISPAKVAEVTKKLYSMGCYEISLGDTIGVG 209

Query: 310 TPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 369
           TPG +  ML  V+  VPV  LAVH HDTYGQ+L N L +LQMG+S +DSSVAGLGGCPYA
Sbjct: 210 TPGAMKDMLSAVLQEVPVTALAVHCHDTYGQALANTLTALQMGVSVMDSSVAGLGGCPYA 269

Query: 370 KGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAIA 422
           +GASGN+ATED+VYML GLGI T V+L+K++ AG FI   L R + SK A A
Sbjct: 270 QGASGNLATEDLVYMLAGLGIHTGVNLQKLLEAGAFICQALNRRTNSKVAQA 321

[Close]

BLAST of Sed0006385 vs. ExPASy TrEMBL
Match: A0A6J1JBX8 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.0e-215
Identity = 381/432 (88.19%), Postives = 400/432 (92.59%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPLGL+KLPSMSTIDR+QRFSSGACRPIGDDMGMGI WI+G RSSSSN CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKDTSQ  SVNRRTMS G +  VSG+FCDSHCSP HEY+ KSSS N QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 120

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
             NK L  +PKFVKIVEVGPRDGLQNEK MVPTFVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDAA+VMEAV NLEG RLPVLTPN+KGF+AA+ AGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEE LDRYRAVTSAAKEL IPVRGYVSCVVGCPTEG+IPPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDL KVMSAG+FI+NHLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALSNVAVDSSMM 433
           A + +A D+S M
Sbjct: 421 AFNQLAADASKM 432

[Close]

BLAST of Sed0006385 vs. ExPASy TrEMBL
Match: A0A6J1JE93 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.0e-215
Identity = 381/432 (88.19%), Postives = 400/432 (92.59%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPLGL+KLPSMSTIDR+QRFSSGACRPIGDDMGMGI WI+G RSSSSN CKEYDE
Sbjct: 22  MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 81

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKDTSQ  SVNRRTMS G +  VSG+FCDSHCSP HEY+ KSSS N QH
Sbjct: 82  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 141

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
             NK L  +PKFVKIVEVGPRDGLQNEK MVPTFVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 142 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 201

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDAA+VMEAV NLEG RLPVLTPN+KGF+AA+ AGAKEVAVFASASESFSKSNIN
Sbjct: 202 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 261

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEE LDRYRAVTSAAKEL IPVRGYVSCVVGCPTEG+IPPSKVAYVAKELYEMGCFEI
Sbjct: 262 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 321

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 322 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDL KVMSAG+FI+NHLGRPSGSKT+I
Sbjct: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 441

Query: 421 ALSNVAVDSSMM 433
           A + +A D+S M
Sbjct: 442 AFNQLAADASKM 453

[Close]

BLAST of Sed0006385 vs. ExPASy TrEMBL
Match: A0A6J1JXR7 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111490728 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.6e-214
Identity = 381/430 (88.60%), Postives = 402/430 (93.49%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSS+EEPLGL+KLPSMSTIDRIQRFSSGACRPIGDD+GMGI WIEG RSS SN C+EYDE
Sbjct: 1   MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGMGICWIEGGRSSLSNSCREYDE 60

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKD SQK SVN+RTMS+GSK FV+GDFCDSHCS S EYQ +SSS + Q+
Sbjct: 61  DARETFPWRRHRKDASQKGSVNKRTMSIGSKKFVTGDFCDSHCSSSPEYQTESSSHHIQN 120

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
           M NK  NG+PKFVKIVEVGPRDGLQNEKDMVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MANKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME V NLEGVRLPVLTPNMKGF+AA+AAGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPP KVAYVAK+LY+MGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPYKVAYVAKQLYDMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDL KV+SAG+FINNHLGRPSGSKTAI
Sbjct: 361 AGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKVISAGNFINNHLGRPSGSKTAI 420

Query: 421 ALSNVAVDSS 431
           AL  VA D+S
Sbjct: 421 ALGRVAADAS 430

[Close]

BLAST of Sed0006385 vs. ExPASy TrEMBL
Match: A0A6J1K101 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111490728 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.6e-214
Identity = 381/430 (88.60%), Postives = 402/430 (93.49%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSS+EEPLGL+KLPSMSTIDRIQRFSSGACRPIGDD+GMGI WIEG RSS SN C+EYDE
Sbjct: 20  MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGMGICWIEGGRSSLSNSCREYDE 79

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKD SQK SVN+RTMS+GSK FV+GDFCDSHCS S EYQ +SSS + Q+
Sbjct: 80  DARETFPWRRHRKDASQKGSVNKRTMSIGSKKFVTGDFCDSHCSSSPEYQTESSSHHIQN 139

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
           M NK  NG+PKFVKIVEVGPRDGLQNEKDMVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 140 MANKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 199

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME V NLEGVRLPVLTPNMKGF+AA+AAGAKEVAVFASASESFSKSNIN
Sbjct: 200 RVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAAGAKEVAVFASASESFSKSNIN 259

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPP KVAYVAK+LY+MGCFEI
Sbjct: 260 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPYKVAYVAKQLYDMGCFEI 319

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 320 SLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 379

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDL KV+SAG+FINNHLGRPSGSKTAI
Sbjct: 380 AGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKVISAGNFINNHLGRPSGSKTAI 439

Query: 421 ALSNVAVDSS 431
           AL  VA D+S
Sbjct: 440 ALGRVAADAS 449

[Close]

BLAST of Sed0006385 vs. ExPASy TrEMBL
Match: A0A6J1K335 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111490728 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.6e-214
Identity = 381/430 (88.60%), Postives = 402/430 (93.49%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSS+EEPLGL+KLPSMSTIDRIQRFSSGACRPIGDD+GMGI WIEG RSS SN C+EYDE
Sbjct: 60  MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGMGICWIEGGRSSLSNSCREYDE 119

Query: 61  DARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQH 120
           DAR+TFPWRR RKD SQK SVN+RTMS+GSK FV+GDFCDSHCS S EYQ +SSS + Q+
Sbjct: 120 DARETFPWRRHRKDASQKGSVNKRTMSIGSKKFVTGDFCDSHCSSSPEYQTESSSHHIQN 179

Query: 121 MGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180
           M NK  NG+PKFVKIVEVGPRDGLQNEKDMVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 180 MANKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 239

Query: 181 RVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME V NLEGVRLPVLTPNMKGF+AA+AAGAKEVAVFASASESFSKSNIN
Sbjct: 240 RVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAAGAKEVAVFASASESFSKSNIN 299

Query: 241 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPP KVAYVAK+LY+MGCFEI
Sbjct: 300 CSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPYKVAYVAKQLYDMGCFEI 359

Query: 301 SLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLE VMA VPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 360 SLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 419

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDL KV+SAG+FINNHLGRPSGSKTAI
Sbjct: 420 AGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKVISAGNFINNHLGRPSGSKTAI 479

Query: 421 ALSNVAVDSS 431
           AL  VA D+S
Sbjct: 480 ALGRVAADAS 489

[Close]

BLAST of Sed0006385 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 581.6 bits (1498), Expect = 5.1e-166
Identity = 300/432 (69.44%), Postives = 350/432 (81.02%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPL  +KLPSMST+DRIQRFSSGACRP  DD+GMG  WIEG   ++SN C + D+
Sbjct: 1   MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 60

Query: 61  D-ARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQ 120
             A+++FPWRR  +  S+   + R     G  + VSG   +S      +Y   S+   T 
Sbjct: 61  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 120

Query: 121 HMGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSP 180
           H+ NK+  G+PKFVKIVEVGPRDGLQNEK++VPT VKVELI RLVSSGLPVVEATSFVSP
Sbjct: 121 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 180

Query: 181 TRVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNI 240
             VPQL+DA  VM+AV  L+G RLPVLTPN+KGFQAA++AGAKEVA+FASASESFS SNI
Sbjct: 181 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 240

Query: 241 NCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE  +PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 241 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 300

Query: 301 ISLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIG+ TPG+VVPMLE VMA VP DKLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 301 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 360

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTA 420
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AGDFI+ HLGRP+GSK A
Sbjct: 361 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 420

Query: 421 IALS-NVAVDSS 431
           +AL+  +  D+S
Sbjct: 421 VALNRRITADAS 431

[Close]

BLAST of Sed0006385 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 581.6 bits (1498), Expect = 5.1e-166
Identity = 300/432 (69.44%), Postives = 350/432 (81.02%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPL  +KLPSMST+DRIQRFSSGACRP  DD+GMG  WIEG   ++SN C + D+
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 95

Query: 61  D-ARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQ 120
             A+++FPWRR  +  S+   + R     G  + VSG   +S      +Y   S+   T 
Sbjct: 96  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 155

Query: 121 HMGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSP 180
           H+ NK+  G+PKFVKIVEVGPRDGLQNEK++VPT VKVELI RLVSSGLPVVEATSFVSP
Sbjct: 156 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 215

Query: 181 TRVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNI 240
             VPQL+DA  VM+AV  L+G RLPVLTPN+KGFQAA++AGAKEVA+FASASESFS SNI
Sbjct: 216 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 275

Query: 241 NCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE  +PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 276 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 335

Query: 301 ISLGDTIGVATPGTVVPMLEVVMAAVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIG+ TPG+VVPMLE VMA VP DKLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 336 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 395

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLEKVMSAGDFINNHLGRPSGSKTA 420
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AGDFI+ HLGRP+GSK A
Sbjct: 396 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 455

Query: 421 IALS-NVAVDSS 431
           +AL+  +  D+S
Sbjct: 456 VALNRRITADAS 466

[Close]

BLAST of Sed0006385 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 9.4e-112
Identity = 210/316 (66.46%), Postives = 245/316 (77.53%), Query Frame = 0


Query: 1   MSSLEEPLGLEKLPSMSTIDRIQRFSSGACRPIGDDMGMGIYWIEGWRSSSSNICKEYDE 60
           MSSLEEPL  +KLPSMST+DRIQRFSSGACRP  DD+GMG  WIEG   ++SN C + D+
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 95

Query: 61  D-ARDTFPWRRIRKDTSQKASVNRRTMSVGSKNFVSGDFCDSHCSPSHEYQNKSSSGNTQ 120
             A+++FPWRR  +  S+   + R     G  + VSG   +S      +Y   S+   T 
Sbjct: 96  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 155

Query: 121 HMGNKVLNGMPKFVKIVEVGPRDGLQNEKDMVPTFVKVELIHRLVSSGLPVVEATSFVSP 180
           H+ NK+  G+PKFVKIVEVGPRDGLQNEK++VPT VKVELI RLVSSGLPVVEATSFVSP
Sbjct: 156 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 215

Query: 181 TRVPQLSDAAQVMEAVCNLEGVRLPVLTPNMKGFQAAIAAGAKEVAVFASASESFSKSNI 240
             VPQL+DA  VM+AV  L+G RLPVLTPN+KGFQAA++AGAKEVA+FASASESFS SNI
Sbjct: 216 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 275

Query: 241 NCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE  +PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 276 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 335

Query: 301 ISLGDTIGVATPGTVV 316
           ISLGDTIG+ TPG  V
Sbjct: 336 ISLGDTIGIGTPGNFV 350

The following BLAST results are available for this feature:

BLAST of Sed0006385 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985653.1	1.2e-214	88.19	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita maxima]	[more]
XP_022985654.1	1.2e-214	88.19	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP...	[more]
XP_023512797.1	4.7e-214	88.19	hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_...	[more]
XP_022995070.1	1.4e-213	88.60	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita maxima...	[more]
XP_022995072.1	1.4e-213	88.60	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X3 [Cucurbita maxima...	[more]

BLAST of Sed0006385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81027	7.2e-165	69.44	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G...	[more]
P35915	5.8e-106	66.10	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL...	[more]
A8WG57	7.5e-106	64.86	3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgc...	[more]
Q8JZS7	2.9e-105	62.71	3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hm...	[more]
Q29448	4.1e-104	66.10	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Bos taurus OX=9913 GN=HMGCL PE...	[more]

BLAST of Sed0006385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBX8	6.0e-215	88.19	3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11148364...	[more]
A0A6J1JE93	6.0e-215	88.19	3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11148364...	[more]
A0A6J1JXR7	6.6e-214	88.60	3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11149072...	[more]
A0A6J1K101	6.6e-214	88.60	3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11149072...	[more]
A0A6J1K335	6.6e-214	88.60	3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11149072...	[more]

BLAST of Sed0006385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26800.1	5.1e-166	69.44	Aldolase superfamily protein	[more]
AT2G26800.2	5.1e-166	69.44	Aldolase superfamily protein	[more]
AT2G26800.3	9.4e-112	66.46	Aldolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 32..330 e-value: 3.5E-113 score: 379.8
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 38..311 e-value: 1.7E-56 score: 191.7
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 38..305 score: 34.167904
None	No IPR available	PANTHER	PTHR42738:SF15	HYDROXYMETHYLGLUTARYL-COA LYASE	coord: 27..335
None	No IPR available	CDD	cd07938	DRE_TIM_HMGL	coord: 40..311 e-value: 5.28042E-172 score: 476.885
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 33..330
IPR043594	HMG-CoA lyase	PANTHER	PTHR42738	HYDROXYMETHYLGLUTARYL-COA LYASE	coord: 27..335
IPR000138	Hydroxymethylglutaryl-CoA lyase, active site	PROSITE	PS01062	HMG_COA_LYASE	coord: 264..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0006385.1	Sed0006385.1	mRNA
Sed0006385.2	Sed0006385.2	mRNA
Sed0006385.3	Sed0006385.3	mRNA
Sed0006385.4	Sed0006385.4	mRNA
Sed0006385.5	Sed0006385.5	mRNA
Sed0006385.6	Sed0006385.6	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046951	ketone body biosynthetic process
biological_process	GO:0006552	leucine catabolic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004419	hydroxymethylglutaryl-CoA lyase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016833	oxo-acid-lyase activity