Homology
BLAST of Sed0006163 vs. NCBI nr
Match:
XP_038877586.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Benincasa hispida])
HSP 1 Score: 2745.3 bits (7115), Expect = 0.0e+00
Identity = 1405/1817 (77.33%), Postives = 1536/1817 (84.53%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENK+SELVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKISELVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCTSNFI APSTD +N E+SEKIRVCNYCFKQ G+EP LQDGDKATSSAI
Sbjct: 61 RCGRVFCSKCTSNFIPAPSTDPSNAREESEKIRVCNYCFKQWGKEPSDLQDGDKATSSAI 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
S SSSS+SM S+KS YTC SA+SN STPCSN HQYYDPYC ITDEQD+LR+GK+INR
Sbjct: 121 SRSSSSTSMGSTKSGYTCHSAISNSASTPCSNGHQYYDPYCPGAITDEQDNLRTGKTINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S +Y GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN DEGLNL+
Sbjct: 181 TTSLMTSSSSFY--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLER 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
EN TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK+NEPMDFQNNG+L
Sbjct: 241 ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYVMQNKDNEPMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKWA-PSFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
WLPPEPEDEED+K+L F+DD+D+ +PG+W SL SGE MKG+SSE+Y+KTAK VVE
Sbjct: 301 WLPPEPEDEEDEKVLLFEDDDDACAPGEWGYLHSSLSSGECHMKGKSSEDYKKTAKNVVE 360
Query: 361 GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
GHFRALV QLLEAE+LP+GD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361 GHFRALVSQLLEAENLPVGDEPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420
Query: 421 KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421 KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480
Query: 481 QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIASCTG
Sbjct: 481 QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTG 540
Query: 541 ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
A+IIPTVDHVASLKLGYCDAFRVETF E HD GDGQ K S KTLMFFEGCPKPLCCT+L
Sbjct: 541 ANIIPTVDHVASLKLGYCDAFRVETFVEEHDNGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600
Query: 601 LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
LRGAS +ELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP KSP+TV LPHNPSN
Sbjct: 601 LRGASGEELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNI 660
Query: 661 DRSISSIPGFTCPS------PELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEPEF--- 720
DRSIS+IPGFTCPS PE EIGQS KDA+ ++DSSII +NV NL+SLEP
Sbjct: 661 DRSISAIPGFTCPSPVKSPEPEPIMEIGQSGKDAILSRDSSIISHNVVNLNSLEPTLRLL 720
Query: 721 ------------------------------IEANRNLERQEYSIGTMTIKEAVGADDDTI 780
E RNL+R+EYS+G MTIKE G DDDT+
Sbjct: 721 KSNATSSSMSLLKQDVSTADCNYMLSSKFSSEERRNLDRKEYSMGMMTIKEEAGDDDDTV 780
Query: 781 SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
SSGK E S+ N I IS + LD + Q+SNDSTSQ PES AN V+TKSSREDFPLSPS
Sbjct: 781 RSSGKGEPLSRFANSIQISRNLLDANAQSSNDSTSQGPESSNANLVSTKSSREDFPLSPS 840
Query: 841 DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
DQQNILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLG+FLRDNLFDQ+YRCHSC MPS
Sbjct: 841 DQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGQFLRDNLFDQNYRCHSCGMPS 900
Query: 901 EAHVHCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
EAHVHCYTHPQGSLTISAKKL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+
Sbjct: 901 EAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSD 960
Query: 961 --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYY+AINVQSV I
Sbjct: 961 AAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYAAINVQSVHI 1020
Query: 1021 PPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHY 1080
PP ++EFHH NLEWINKEANEVHNRAKTLFAEV KALPLI +ESGTEQEDFGVNEL++Y
Sbjct: 1021 PPSMIEFHHNNLEWINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELNNY 1080
Query: 1081 VQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFL 1140
+QELEQI+NMEKE+FEVHFNEVL SGE +GQ VDIF+INKLHMH+LFL
Sbjct: 1081 IQELEQIVNMEKENFEVHFNEVL-----------SGETTVGQSAVDIFQINKLHMHVLFL 1140
Query: 1141 SYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDND 1200
SY WVQRL+Y LRKK+S DSSSNDIP ++++ELDSLE+LV+FTL S RDEFF GFDND
Sbjct: 1141 SYIWVQRLNYVGTLRKKHSSDSSSNDIPAIEDKELDSLEELVEFTLASGRDEFFGGFDND 1200
Query: 1201 SLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIR 1260
SLP ESK E+DL GG SG+IS+SNE REKN DLGG EDDLC S PVN P DV I I+
Sbjct: 1201 SLPAESKREVDLRRGGNSGKISRSNEANREKNIDLGGQEDDLCFS-PVNFEPLDVGIGIQ 1260
Query: 1261 RANSEGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNA 1320
RANS GEYPIM D S TLD VWKGVI PNN FE+ S S TN A+LEPL+GQSD E+C A
Sbjct: 1261 RANSVGEYPIMTDFSGTLDEVWKGVIHPNNVIFED-SYSRTNFAILEPLAGQSDTERCTA 1320
Query: 1321 TLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFIS 1380
GVET H LH+A+VLTRDDSVETITSST++PSSAY +KISLLNIPK +ISDYD AFIS
Sbjct: 1321 N--GVETTHFLHAALVLTRDDSVETITSSTSIPSSAYNTKISLLNIPKSDISDYDPAFIS 1380
Query: 1381 SFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDT 1440
S+RELEKQ HLRLLMHVGSSDTV+PVYDEEPTSI+AYSLLMPEY QMSE EK +E DT
Sbjct: 1381 SYRELEKQSHLRLLMHVGSSDTVLPVYDEEPTSIVAYSLLMPEYIAQMSEKEKGMEASDT 1440
Query: 1441 SRSLPIIGSAKLSSLKTSDEEGSG----------TILSMPKTE-RQFEDRVSIAQNKHTR 1500
+RSLPIIGSAK++ ++T DEEGS TILSMP+TE RQFE+ +S ++ HTR
Sbjct: 1441 ARSLPIIGSAKMNLIRTFDEEGSDAYKTLRSNEETILSMPRTESRQFENLLSFTKDLHTR 1500
Query: 1501 VSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFA 1560
V FVNETPLGPVKYT+T +FAKRFEALRK CCP+ELDY+RSLSRCKRWGAQGGKSNVFFA
Sbjct: 1501 VCFVNETPLGPVKYTVTCYFAKRFEALRKMCCPTELDYIRSLSRCKRWGAQGGKSNVFFA 1560
Query: 1561 KTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGG 1620
KTLDERFIIKQVTKIEL+SFIQFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+LKGG
Sbjct: 1561 KTLDERFIIKQVTKIELESFIQFARAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGG 1620
Query: 1621 KECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFL 1680
KECK+D+LVMENLLFRHNVT+LYDLKGSSR+RYNSDTSGKN VLLDQNLIESMPTSPIFL
Sbjct: 1621 KECKVDVLVMENLLFRHNVTRLYDLKGSSRARYNSDTSGKNKVLLDQNLIESMPTSPIFL 1680
Query: 1681 GSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETW 1740
GSKAKRLLERAVWNDTSFLAS+ VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW
Sbjct: 1681 GSKAKRLLERAVWNDTSFLASVDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETW 1740
Query: 1741 LKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDG 1748
+KNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPS+TVSPSESHPDLCD+
Sbjct: 1741 VKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSTTVSPSESHPDLCDEN 1798
BLAST of Sed0006163 vs. NCBI nr
Match:
KAG7020853.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1399/1798 (77.81%), Postives = 1527/1798 (84.93%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP LQDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+ KSINR
Sbjct: 121 SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS V+FD CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L DDD+DS +PG+W PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DSSII NNV NL+S L+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720
Query: 721 FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
+ + +Q+ SI + + DDDT+ SGK E SSQ N I IS
Sbjct: 721 ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780
Query: 781 IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
++LDG+TQASN STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT
Sbjct: 781 SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840
Query: 841 CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841 CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900
Query: 901 KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
KL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+ FSNNA
Sbjct: 901 KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960
Query: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020
Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
NEVHNRA+TLFAEV KALPLI E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080
Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
NEVL SGE +GQP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140
Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
P SSSND+ LDER ++S+E+ V+FTL +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200
Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
QISQ NE EREK+ DLG LEDDLCLSP +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260
Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
AVWKGV+ PN+ T +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320
Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
DSVET TS +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380
Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
+DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440
Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
EE + TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500
Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560
Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620
Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740
Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778
BLAST of Sed0006163 vs. NCBI nr
Match:
XP_023520487.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1401/1798 (77.92%), Postives = 1527/1798 (84.93%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP LQDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+S+ SSKSDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+GKSINR
Sbjct: 121 SLSSSSTSIGSSKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE ++ISQSD+YYC VNADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSECRNISQSDNYYCDVNADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS V+FD CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L DDD+DS +PG+W PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DSSII NNV NL+S L+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720
Query: 721 FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
+ +L +Q+ SI + + DDDT+ SGK E SSQ N I IS
Sbjct: 721 ASSTSLSLLKQDVSISDNNMLSSKFTSEANIRLDNKDDDTVPISGKGEVSSQLRNSIYIS 780
Query: 781 IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
++LDG+TQASN STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT
Sbjct: 781 SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840
Query: 841 CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841 CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900
Query: 901 KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
KL F E EGKIWMWHRCLKCPRTNGFPPATRR+I+ FSNNA
Sbjct: 901 KLPDFFLPGESEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960
Query: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020
Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
NEVHNRA+TLFAEV KALPLI E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080
Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
NEVL SGE +GQP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140
Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
P SSSND+ LDER ++S+E+ V+FTL +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200
Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
QISQ NE EREK+ DLG LEDDLCLSP +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260
Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
AVWKGV+ PN+ T +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320
Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
DSVET TS +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380
Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
+DTVVPVYDEEPTSIIAYSLL PEYN QM+EPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMAEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440
Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
EE + TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500
Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560
Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGGKECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGKECKLDILVMENLLFRHNV 1620
Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740
Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEESSEPEHSWDGKPYRYQSD 1778
BLAST of Sed0006163 vs. NCBI nr
Match:
KAG6588292.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2711.8 bits (7028), Expect = 0.0e+00
Identity = 1399/1798 (77.81%), Postives = 1527/1798 (84.93%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP LQDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+ KSINR
Sbjct: 121 SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS V+FD CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L DDD+DS +PG+W PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DSSII NNV NL+S L+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720
Query: 721 FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
+ + +Q+ SI + + DDDT+ SGK E SSQ N I IS
Sbjct: 721 ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780
Query: 781 IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
++LDG+TQASN STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT
Sbjct: 781 SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840
Query: 841 CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841 CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900
Query: 901 KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
KL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+ FSNNA
Sbjct: 901 KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960
Query: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020
Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
NEVHNRA+TLFAEV KALPLI E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080
Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
NEVL SGE +GQP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140
Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
P SSSND+ LDER ++S+E+ V+FTL +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200
Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
QISQ NE EREK+ DLG LEDDLCLSP +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260
Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
AVWKGV+ PN+ T +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320
Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
DSVET TS +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380
Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
+DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440
Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
EE + TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500
Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560
Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620
Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740
Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778
BLAST of Sed0006163 vs. NCBI nr
Match:
XP_016899810.1 (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])
HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1397/1814 (77.01%), Postives = 1525/1814 (84.07%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENK+S+LVGL KSW+ WRS+ NV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKISDLVGLVKSWM-PWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+NFI A STD TN EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61 RCGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
S+SSSS+SM S+KS YTC SA+S+I STPCS S QYYDPYCS I DE D+LR+GK+INR
Sbjct: 121 SVSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S Y GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN D GLNL+
Sbjct: 181 TTSLMTSSSSYC--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLER 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
EN TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK++EPMDFQNNG+L
Sbjct: 241 ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKWAP-SFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
WLPPEPEDEED+K L F+DD+D +PG+W SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301 WLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360
Query: 361 GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
GHFRALV QLLEAE+LPIGD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361 GHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420
Query: 421 KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421 KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480
Query: 481 QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481 QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540
Query: 541 ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ K S KTLMFFEGCPKPLCCT+L
Sbjct: 541 ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600
Query: 601 LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNI 660
Query: 661 DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
DRSIS+IPGFTC P PE EIGQS KDAV ++D SII +NV NL+SLEP +
Sbjct: 661 DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLL 720
Query: 721 EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
E+N R+L+ +EYS+G MTIKE D D +SSSGK
Sbjct: 721 ESNATSTSFSFLKQDFSTMLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSGK 780
Query: 781 DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
EASS+ N I +S +SLD +TQ+ NDSTSQ PE+ + VTTKSSREDFPLSPSDQQN
Sbjct: 781 REASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQN 840
Query: 841 ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841 ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900
Query: 901 HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
HCYTHPQGSLTISAKKL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+
Sbjct: 901 HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960
Query: 961 ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961 LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020
Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
+EFH +NLEWINKEANEVHNRAKTLF EV KALPLIS +ESG EQEDFG +EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQEL 1080
Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
EQIINMEKE+FEVHFN+VL SG + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKVL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140
Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
VQRL+Y A LRKK+SPD SSNDI L+E+EL+SLE+LV+ TL S RDEFF G DNDSLP
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPP 1200
Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
ESK E LG G SG IS+ NE REKN DLGG EDD CLS PVNS P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLS-PVNSEPVDVGIGIRRANS 1260
Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
GEYPIM DLS TLDAVWKGVI PN+ FE+S S TNSA+LEPL+GQSDMEKC A G
Sbjct: 1261 VGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF-SRTNSAILEPLAGQSDMEKCTAN--G 1320
Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
+ETAHLLHSA+VLT DDSVETIT+ST+MP SAY +KISLLNIPK +ISDY FISS+RE
Sbjct: 1321 IETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380
Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEY QMSEP K +E DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSL 1440
Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
P+IGS K++S+KT DEEGS TILSMP+TE QFED V + ++ HTRV FV
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFV 1500
Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560
Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620
Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
+D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSG N VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKA 1680
Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740
Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1795
BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match:
Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)
HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1133/1808 (62.67%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
M ++K V + KSWI +S S N++RDFWMPD+SC VCYECD+QFT RRHHCR
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFC+KC +N I +PS ++ + E+ E+IRVCNYC+KQ + +G +S I
Sbjct: 61 LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SL SSS S + T S+ I+ST + +P S ++ DS +S + N
Sbjct: 121 SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180
Query: 181 TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
+ R++ G+ D G G+ +DDD Y + + +Q +DYY
Sbjct: 181 KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240
Query: 241 CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
A+N DE ++ G ++ K I P + + L++E D EN +
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300
Query: 301 FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
++ E +DF+++G+LWLPPEPE+EED++ + DDD D G W PS S
Sbjct: 301 GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360
Query: 361 LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
++ K +SS K VVEGHFRALV QLLE ++LP+ + +GWLDIIT LS
Sbjct: 361 FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420
Query: 421 WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
WEAAT+LKPD SKSG MDP GYVKVKC+ G+RSES++VKG++CKKNVAH+RMT +I K
Sbjct: 421 WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480
Query: 481 RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481 RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540
Query: 541 LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F Q
Sbjct: 541 LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600
Query: 601 ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
+K AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY FAAYHLALET FLADE
Sbjct: 601 VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660
Query: 661 GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
GAS+ ELP ++P+TV LP PS +RSIS+IPGFT P+ EL E ++ D
Sbjct: 661 GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720
Query: 721 NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
N SS + + D ++P ER +++ T+ K E + + DD + + +
Sbjct: 721 NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780
Query: 781 DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
S S+ D L G+T+ D + N +SS+ DF
Sbjct: 781 SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840
Query: 841 SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ C SC+
Sbjct: 841 SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900
Query: 901 MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
MP+EAH+HCYTH QGSLTIS KKL L + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901 MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960
Query: 961 ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961 SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020
Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
+PP L F++ N EW+ KE+ EV +A+ LF EV +AL IS G + N++
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080
Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
++EL ++ K++++ ++L+ V GQP +DI INKL I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLII 1140
Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
F SY W + L+ AA + + N ++ N P + R + SLE+L ++S
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200
Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
NDSL ++ +E L G S + D+G C P V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260
Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
E S ++ A++E ++ DLSDTLDA W G NG F S + + + P
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320
Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
+ L+G E S + + + T+ +PS ++Y +K LN K
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380
Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
+++ ++SS+RELE + RLL+ +G +D V+PVYD+EPTSIIAY+L EY QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440
Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
+K+ + D+ S + S L SL + + S+ + Q + + ++
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500
Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
H R+SF +E P G VKY++T ++AK FEALR CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560
Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
FFAK+LD+RFIIKQVTK EL+SFI+F AYF YL+E+ T PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620
Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680
Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740
BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match:
Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)
HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 882/1830 (48.20%), Postives = 1150/1830 (62.84%), Query Frame = 0
Query: 9 SELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCS 68
SE+VGL KSW+ WRS V+RDFWMPD+SCRVCYECD QFT I RRHHCR CGRVFC
Sbjct: 12 SEIVGLIKSWL-PWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCG 71
Query: 69 KCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAISLSSSSS- 128
KCT+N I +D ED E+IRVCNYCF+Q + DG S+ LS+S S
Sbjct: 72 KCTANSIPFAPSDLRTPREDWERIRVCNYCFRQ-----WEQGDGGPHVSNITELSTSPSE 131
Query: 129 -SMDSSKSDYTCQSALSNINSTP-----CSNSHQYYDPYCSFPITDEQDSLRSGKSINRT 188
S+ SSK+ T S+ + S P H D + E + GK +R
Sbjct: 132 TSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRR 191
Query: 189 TPRTAS---TSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNL 248
+ A+ + R DDE D+ YG Y++D ET H ++++YY + + G+ +
Sbjct: 192 SSFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYN-GMGI 251
Query: 249 D--------GENICTK---GSAVIFDTPCPRSNEELSSED---SDENDKGSECD--FPAY 308
D GE K GS +I C S SE E+D EC+ PA
Sbjct: 252 DDVPCKHLGGETADQKSLSGSPLIHQ--CLESLIREGSEQFQKKSEHDGRDECEASSPAD 311
Query: 309 GMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK--LLFFDDDNDSRSPGKWA---PSFSLGS 368
++ EP+DF+NNG+LW+PPEPE+EED++ LF ++DN+ + G+W PS S GS
Sbjct: 312 ISDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGS 371
Query: 369 GEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEA 428
GEY + R++EE++K K VV+GHFRAL+ QLL+ E++ + D + WL+IIT LSWEA
Sbjct: 372 GEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEA 431
Query: 429 ATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIF 488
A +LKPDMSKSG MDP GYVKVKC+A G R +S++VKG++CKKNV ++RM+ +I K+R+
Sbjct: 432 ANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLL 491
Query: 489 VLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVA 548
+LGG LEYQRV N +SS DTL+QQE DHLKMAVAKI A PNILLVE+SVSR AQEYL+A
Sbjct: 492 ILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLA 551
Query: 549 KNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASK 608
K+I++VLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + GQ K
Sbjct: 552 KDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGK 611
Query: 609 TSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
KTLM+FE CPKPL T+LLRGA++DELKKVKHVVQY FAAYHLALET FLADEGAS
Sbjct: 612 KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671
Query: 669 VPELPSKSPLTVILPHNPSNTDRSISSIPGFTC----PSPELNTEIGQSRKDAVPNKDSS 728
PELP SP+TV LP ++ +RSIS++PGFT SP + + R ++VP +
Sbjct: 672 -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELL 731
Query: 729 IIRNNVANLDSLEP-------EFIEANRNLERQEYSIGTMTIKEAV--GADDDTISSSGK 788
N++ + P + E N + ++I ++ + + V + D S
Sbjct: 732 STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNI-SLNLPDRVIESRNSDLSGRSVP 791
Query: 789 DEASSQRCNPISISIDSLDGSTQAS-------------------NDSTSQRPESPKAN-- 848
+ + + NPI ++ ++ + S S D+ S+ + + N
Sbjct: 792 VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 851
Query: 849 -PVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLR 908
P T+S +E+FP SPSD Q+ILVSLS+R VWKGT C+R+HL RIKYYGSFD+PLGRFLR
Sbjct: 852 KPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLR 911
Query: 909 DNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLT-LFYREPEGKIWMWHRCLKC 968
D+LFDQSYRC SC MPSEAHVHCYTH QGSLTIS KKL L E EGKIWMWHRCL+C
Sbjct: 912 DHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRC 971
Query: 969 PRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGR 1028
PR NGFPPAT R+++ FSN+AAASRVA CGH LHRDCLRFYGFG
Sbjct: 972 PRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGN 1031
Query: 1029 TVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNS 1088
VACF Y+ I+V SV +PP IL F++ N +WI +E +EV RA+ LF+EV A+ I +
Sbjct: 1032 MVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI--A 1091
Query: 1089 ESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQP 1148
E G + + ELE+++ EK +FE + ++L E+ NE GQP
Sbjct: 1092 EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREV---NE--------GQP 1151
Query: 1149 RVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVK 1208
VDI E+ ++H +LF SY W RL A+ L K + D + +RE + L K
Sbjct: 1152 LVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDT--------KREENEKPPLAK 1211
Query: 1209 FTLESRRDEFFDGFDNDSLPLESKHEIDLG-NGGYSGQISQSNEMERE--KNFDLGGLED 1268
+ + + L + E++L +G +G N +++E N DL +D
Sbjct: 1212 -------SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKD 1271
Query: 1269 DLC-LSP----PVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQ-----PNN 1328
D +SP P S P + ++ +RR S+G+ +M +LS TLDA W G Q P N
Sbjct: 1272 DGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTN 1331
Query: 1329 GTFEESSNSGTNSAVLEPLSG---QSDM-EKCNATLVGVETAHLLHSAIVLTRDDSVETI 1388
++ +NS+ P+S D+ E+ N V + L S +DSV
Sbjct: 1332 NKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSV--- 1391
Query: 1389 TSSTAMPSSAYYSKIS---LLNIPK-PNISDYDHAFISSFRELEKQEHLRLLMHVGSSDT 1448
S ++P +Y I+ LL+ K ++ +ISSFRE E Q RLL+ VG +D
Sbjct: 1392 -SWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDI 1451
Query: 1449 VVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEG 1508
VVPVYD+EPTS+IAY+L+ PEY Q S ++L + ++P + S+
Sbjct: 1452 VVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNGSV 1511
Query: 1509 SGTILSMPKT-ERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1568
+ILS+ + D +S + H RVS+ + LG VKYT+T ++AKRFEALR C
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571
Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1628
PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK EL+SFI+FA AYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631
Query: 1629 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1688
E+ T PTCLAK++GIYQV++K LK GKE K+D+L+MENLLF V +LYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691
Query: 1689 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1733
YN D+SG N VLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLA VMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751
BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match:
Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)
HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-285
Identity = 651/1799 (36.19%), Postives = 958/1799 (53.25%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNS----ENVARDFWMPDRSCRVCYECDSQFTFIKRR 60
MG P+ L +L+ +SWI+S S+S + +DF + ++C++C T +++
Sbjct: 1 MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60
Query: 61 HHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYC---FKQLGREPFALQDGD 120
+ C CG +C C+ + K+++C C ++L + +
Sbjct: 61 YCCLSCGSCWCKSCSDT--------------EESKMKLCRECDAEVRELRVKSYDKVHPR 120
Query: 121 KATSSAISLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLR 180
+ SL++ S S+ SS C+ N+ S C S +E+++
Sbjct: 121 DSPDPPSSLATESESLASSLEIRDCR----NMASIRCYPSR-----------GEEEEARY 180
Query: 181 SGKSINRTTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVN-- 240
GK + + S G E + S + H S + + +
Sbjct: 181 CGKQLLSPSSDNYQDSSDIESGSVSARHE----LFSCKSSAGSSPHDSPLRNNFSPLGRF 240
Query: 241 ADEGLNLDGENICT---KGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKN 300
+L +C+ ++ D L ED +E ++K
Sbjct: 241 VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------EDKL 300
Query: 301 NEPMDFQNNGVLWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG---SGEYPMKG 360
+P+DF+NNG +W PP PEDE DD + +F D++ G A FSL S P K
Sbjct: 301 QQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIPTKE 360
Query: 361 RSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPD 420
+ E + + VV HFRALV +LL E+L D S WLDI+T L+W+AA +KPD
Sbjct: 361 KLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPD 420
Query: 421 MSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALE 480
G MDP YVK+KCVA G ++ESI+++GI+C KN+ HKRM + R+ +L G+LE
Sbjct: 421 TRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLE 480
Query: 481 YQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVL 540
YQRV ++S +TL+QQE +H+K +AKI++ PN+LLVE+S S +AQ+YL+ K I++VL
Sbjct: 481 YQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVL 540
Query: 541 NIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVE-TFEVHDTGDGQASKTSAKTL 600
N+KRSLL+RIA CTGA + P++D +++ +LG+C+ FR E E H+ G+ Q+++ ++TL
Sbjct: 541 NVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTL 600
Query: 601 MFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPS 660
M+FEGCP+ L CTV+LRG+ ++ELKKVKHV+QYA FAAYHL+LET FLADEGAS+P++
Sbjct: 601 MYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRL 660
Query: 661 KSPLTV--------------ILPHNPSNTD-----RSISSIPGFTCPSPELNTEIGQSR- 720
K P V ++ +P+ TD + + T P PE E+ +S
Sbjct: 661 KQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPE--HEVCESLC 720
Query: 721 KDAVPNK---DSSIIRNNVANLDSLEPEFIEANRNLERQEYSIGTMT-IKEAVGADDDTI 780
+D P + SS + + D+L +F NL + YS + + E I
Sbjct: 721 EDFDPTQIFPPSSEVETEQS--DTLNGDFA---NNLVTRSYSSNQLNDLHEPTLCLSSEI 780
Query: 781 SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
+ + S + N G + N + + + P+ ++ +
Sbjct: 781 PETPTQQPSGEEDN----------GRGEEENQLVNPQ-DLPQHESFYEDDVSSEYFSAAD 840
Query: 841 DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
Q+ILVS S+RCV K + C+R+ LLRIK+YGSFD+PLGR+L+D+LFD++ C SC
Sbjct: 841 SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900
Query: 901 EAHVHCYTHPQGSLTISAKKLLTL-FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
+AHV CY+H G+LTI+ ++L ++ E +GKIWMWHRCL+C +G PPATRR+++
Sbjct: 901 DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960
Query: 961 --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
FSN+A A+RVA CGH L RDCLRFYGFG VA F YS IN+ +VL+
Sbjct: 961 AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020
Query: 1021 PPPILEF--HHNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNEL-S 1080
PP +LEF H EWI EA E+ + +T++ E+ L + S E E +L S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080
Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
+ ++Q++ EK++++ + + + Q +DI E+N+L ++
Sbjct: 1081 RIIGLIDQLVK-EKDEYDDALQPIFEENLQI------------QGSLDILELNRLRRALM 1140
Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
++ W +L + KK S + +D R E D
Sbjct: 1141 IGAHAWDHQLYLLNSQLKKASVFKTGDD-------------------NAPRNPEMHDPPK 1200
Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEIS 1260
D E E D + + + E N D N P ++
Sbjct: 1201 IDRRMQEGSDERD-----------EQSHTDSEANGD--------------NKDPENIP-- 1260
Query: 1261 IRRANSEGEYPIMADLSDTLDAVWKGVIQ--PNNGTFEESSNSGTNSAVLEPLSGQSDME 1320
S G LS+ +D+ W G Q T E+ ++ L L+ ++
Sbjct: 1261 -----SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQ 1320
Query: 1321 KCNATL-------VGVETAHLLHSAI-VLTRDDSVETITSSTAMPSSAYYSKISLLNIPK 1380
++ + G+ + L S + + YS++ L + K
Sbjct: 1321 SFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQK 1380
Query: 1381 PN-ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQ 1440
+ I +ISS ++ + L+ G +D VVPVYD++P S+++Y++ EY
Sbjct: 1381 LDLIVGSAPTYISSASQMADGARM-LIPQRGLNDIVVPVYDDDPASVVSYAINSKEYK-- 1440
Query: 1441 MSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG----TILSMPKTERQF------- 1500
E + G + S+ S+L + E S LSM Q
Sbjct: 1441 ----EWIVNKG-------LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQ 1500
Query: 1501 EDRVS--IAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRC 1560
+DR S + + R S + G VK+++T +FA +F+ LRK CCPSE+D+VRSLSRC
Sbjct: 1501 DDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRC 1560
Query: 1561 KRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAK 1620
+RW AQGGKSNV+FAK+LDERFIIKQV K EL SF FA YF YL E+ + PTCLAK
Sbjct: 1561 QRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAK 1620
Query: 1621 VVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLL 1680
++GIYQVS K+ KGGKE K+D++VMENL + ++++YDLKGS+RSRYN +TSG + VLL
Sbjct: 1621 ILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLL 1648
Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGII 1715
D NL+E++ T PIFLGSKAKR LERA+WNDT+FLAS+ VMDYSLLVG DEE++ELV+GII
Sbjct: 1681 DMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGII 1648
BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match:
Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)
HSP 1 Score: 630.2 bits (1624), Expect = 7.1e-179
Identity = 469/1464 (32.04%), Postives = 726/1464 (49.59%), Query Frame = 0
Query: 300 LWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG--SGEYPMKGRSSEEYRKTAKY 359
+W PPEPE+ ED+ +F DDD+D KW + LG S E K + EE R+
Sbjct: 95 VWEPPEPENPEDEVDGVFADDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLE 154
Query: 360 VVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGY 419
+ F+ +V QL+++ I + W +I+ L WEAA++LKP + +DP+ Y
Sbjct: 155 EADSKFKFIVSQLIKSAGFSIEE---SGYWFEIVARLCWEAASMLKPAIDGKS-VDPTEY 214
Query: 420 VKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLD 479
+KVKC+A G +S + KG++ KK+ A K M + RI ++ G L + I+ SSL
Sbjct: 215 IKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPRIMLVEGVLGHP--ISGFSSLQ 274
Query: 480 TLMQQE---LDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLER 539
++ Q L ++K V I+A P+++LVE+SVSR Q+ ++ K +T+V ++K L+R
Sbjct: 275 SVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQR 334
Query: 540 IASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASKTSAKTLMFFEGCPKPL 599
I+ C G+ I+ +VD ++S KL +CD+FR+E G++ K KTLMF EGCP L
Sbjct: 335 ISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRL 394
Query: 600 CCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILP- 659
CT+LL+G + LKKVK VVQY+ AYHL LE FLAD + +K + ++
Sbjct: 395 GCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEI 454
Query: 660 --HNPSNTDRSISS----IP---GFTCPSPELNTEIGQSRKDAVPNKDSSIIRNNVANLD 719
+PS + R S IP GF + ++N G++ + V +S ++V + +
Sbjct: 455 ENFSPSPSPRESPSEAVDIPVSNGFDEQTIQIN---GEADGEKVGTWESD--GDHVFSHE 514
Query: 720 SLEPEFIEANRNLE-RQEYSIGTMTIKEA--VGADDDTISSSGKDEASSQRCNPISISID 779
P +L R +G + E+ V D D ++S D SI
Sbjct: 515 PYNPVIFTGFSSLSARLSKYLGFVQNPESVPVSVDTDVSTTSNLD------------SIR 574
Query: 780 SLDGSTQASNDSTS------QRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVW 839
+ T N+ + P + ++ KS E+ S + Q+ILV +S R
Sbjct: 575 ESEEDTAEKNEDKQPLLLDPELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNAL 634
Query: 840 KGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLT 899
+G CD+ H IK+Y FD PL +FLRD +F+Q C +C EAH++ Y H LT
Sbjct: 635 RGIMCDQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLT 694
Query: 900 ISAKKLLTL--FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII---------------- 959
I K++ E +GKIWMW RC KC N +T+R++I
Sbjct: 695 IQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELS 754
Query: 960 FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHHNLE-- 1019
FS +R + CGH D L F+G G VA YS + +V +PP LE ++
Sbjct: 755 FSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAG 814
Query: 1020 WINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKE 1079
W+ KE V + +LF + A + S D + +E+++ E+
Sbjct: 815 WLEKEFQTVFTKGISLFED---AAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERC 874
Query: 1080 DFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAA 1139
FE + +KN + I + +N++ +L + W RL +
Sbjct: 875 IFEEN----------IKNSFDKAK-TIDDVSHRLLRLNRMRWELLLQALIWNYRLQ-SLV 934
Query: 1140 LRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSL--PLESKHEID 1199
L + P S+D + E+ L ++ + T ++ D N + PL +I
Sbjct: 935 LSDRLLP---SSDETKIYEQGLKTVSE-AGMTRYENDNKVSDSGSNGGIDTPLVEHKDIP 994
Query: 1200 LGNG--GYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYP 1259
+ G + Q+++S E ++ L D SP N + +++ N +
Sbjct: 995 IAGASVGDNDQMAESYVPEDNESQTLCSSSPD-TTSPINNHFDTHLAVNVHSTNGQEADK 1054
Query: 1260 IMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAH 1319
+ ++LD E S+++G + + E + +V +E +
Sbjct: 1055 SIPVTGESLDD-------------EVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEY 1114
Query: 1320 LLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQE 1379
LL V +++ + + ++ +E
Sbjct: 1115 LLKFEYV-----------------------------------NNFTQENLQTVNQIITEE 1174
Query: 1380 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGS 1439
RL + + D +V Y++E +S+IA +L ++ E +K L S I GS
Sbjct: 1175 SSRLRISLRDDDFIVSDYEDELSSLIACALA----HLNNEESKKPL-------SRCIHGS 1234
Query: 1440 AKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFF 1499
L ++++ T + + + +R+ V+F + +G KY+I +
Sbjct: 1235 --LQGFLDNNQDSKQTDRDVSRFSSESTNRLETLPPPEVLVTFGSVKSVGKPKYSIVSLY 1294
Query: 1500 AKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSF 1559
A F LRKRCC SELDY+ SLSRCK W A+GGKS FAKTLD+RFI+K++ K E +SF
Sbjct: 1295 ADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESF 1354
Query: 1560 IQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLK-GGKECKIDILVMENLLFRHNV 1619
+ FA+ YF Y+ ++ TCLAKV+GI+QV+ + K GGKE + D++VMENL F V
Sbjct: 1355 VTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKV 1414
Query: 1620 TQLYDLKGSSRSRYN-SDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1679
T+ YDLKG+ +R+ + +G++ VLLDQN + M SP+++ +K+ L+RAV+NDTSF
Sbjct: 1415 TRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSF 1450
Query: 1680 LASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVI 1713
L SI+VMDYSLLVG+D+E ELV GIIDY+RQYTWDK LETW+K+S ++ PKN PTVI
Sbjct: 1475 LTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVV--PKNVQPTVI 1450
BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match:
Q9Z1T6 (1-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve PE=1 SV=3)
HSP 1 Score: 364.0 bits (933), Expect = 9.5e-99
Identity = 494/2104 (23.48%), Postives = 801/2104 (38.07%), Query Frame = 0
Query: 31 RDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSE 90
+ +WMPD C+ CY+C +FT +RRHHCR CG++FCS+C + I T
Sbjct: 152 KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTG------ 211
Query: 91 KIRVCNYCFK------------QLGREPFALQDG-------------------------- 150
+R C YC K +G + AL D
Sbjct: 212 DLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIF 271
Query: 151 ---DKATSSAISLSSSSSSM---------DSSKSDYTCQSA-LSNIN----------STP 210
D A S I SS+S++ D+ KS +SA ++N++ S
Sbjct: 272 LEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYE 331
Query: 211 CSNSHQYYDPYCSFPITDEQDSL-----------------RSGKSIN------RTTPR-- 270
S S Q Y T+++ + SG RT P
Sbjct: 332 TSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCI 391
Query: 271 -------------------------TASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKH 330
A G + D D D Y YR T+
Sbjct: 392 VGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRD-EYALYRPLQSTEF 451
Query: 331 ISQSDDYYCAVNADEGL------------NLDGENICTKGSAVIFDTPCPRSNEELSSED 390
+VN+ EG + D E I +G + ++ P +SS
Sbjct: 452 SETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQ 511
Query: 391 SDENDKGSECDFPAYGMQNKN---NEPMDFQNNGVLWLPPEPEDEED-------DKLLFF 450
S + + + N N +P + + +PP P D+++ + L
Sbjct: 512 STVDSDSAASISLNVELDNVNFHIKKPSKYPH-----VPPHPADQKEYLVSDTGGQQLSI 571
Query: 451 DD--------DNDSRSPGKWAPSFSLGSGEYPMKGRSSEEYRKTAKYVV----EGHFRAL 510
D + K P LG ++ E K A + H AL
Sbjct: 572 SDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMAL 631
Query: 511 VGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMS-KSGPMDPSGYVKVKCVAG 570
+ QLL+ E L W DII L + ++PD+ + MD +V +K + G
Sbjct: 632 LQQLLQNESL-------SSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPG 691
Query: 571 GKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEY-QRVINHVSSLDTLMQQEL 630
GK+ +S++V G +C KN+AHK+M I +I +L ++EY R + +D ++ QE
Sbjct: 692 GKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQER 751
Query: 631 DHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTGASII 690
+ LK V +I P ++LVE++VSR AQ+ L+ IT+V+N+K +LERI+ T ++
Sbjct: 752 EFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLV 811
Query: 691 PTVDHVASL-KLGYCDAFRVETFEVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVLLRGA 750
++D + + LG C F ++ F Q KTLMFFEGCP+ L CT+ LRG
Sbjct: 812 VSMDQLLTKPHLGTCHKFYMQIF--------QLPNEQTKTLMFFEGCPQHLGCTIKLRGG 871
Query: 751 SKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNTDRSI 810
S EL +VK ++ + AYH LE FL DE A P L +SP +L S
Sbjct: 872 SDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTL-MQSPSFHLLTEGRGEEGASQ 931
Query: 811 SSIPGFTCPSPELNTEIGQSRKDAVPNKDSSIIRN-NVANLDSLEPEFI-EANRNLERQE 870
+ G + P + ++A+ ++DS+++ + V L+ + I +A +L+ Q+
Sbjct: 932 EQVSGSSLPQDP------ECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQD 991
Query: 871 YSIGT---------------------MTIKEAVGAD------------------------ 930
Y+ T M ++AVG +
Sbjct: 992 YTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDP 1051
Query: 931 --DDTISSSGKDEASSQ--------------------------------------RCN-- 990
DDT ++ SS+ RC+
Sbjct: 1052 LQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTR 1111
Query: 991 ---PISI--------------------------SIDSLDGSTQASN-------------- 1050
P I + ++GS QA +
Sbjct: 1112 DYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRI 1171
Query: 1051 -----DSTS------------QRPESPKANPVT----------------TKSSRED---- 1110
DS + R +S +P T+S E
Sbjct: 1172 AEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIP 1231
Query: 1111 ----FP-----LSPSDQQNILVSLSTRCVWKG---TTCDRAHLLRIKYYGSFDEPLGRFL 1170
+P L+P++ Q + V S+ + C ++ +++YG D LG FL
Sbjct: 1232 SDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFL 1291
Query: 1171 RDNLFDQSYRCHS--CSMPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCL 1230
F SY+C S C P H+ + H QG + I K+L + + I + C
Sbjct: 1292 ERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSP-VPGYQHTILTYSWCR 1351
Query: 1231 KCPRTNGFPPATRRIIIFSNNAAASRVA-------------------GCGHFLHRDCLRF 1290
C + ++ SN + + A CGH +H D ++
Sbjct: 1352 ICKQVT-------PVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQY 1411
Query: 1291 YGFGRTVACFYYSAINVQSVLIPPPILEFHHNLEWINKEANEVHNRAKTLFAEVYKALPL 1350
+ + + VA F YS I + V +P P + +I ++A L + + L
Sbjct: 1412 FSYNQMVASFSYSPIRLLEVCVPLPKI-------FIKRQA--------PLKVSLLQDL-- 1471
Query: 1351 ISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVA 1410
+DF Y+ E++ +++ + F E + +I + E+ GE
Sbjct: 1472 ----------KDFFQKVSQVYLAVDERLASLKTDTFSKTREEKME-DIFAQKEMEEGEFK 1531
Query: 1411 IGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLE 1470
W +++ ++P + L ++ E
Sbjct: 1532 -----------------------NWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCE 1591
Query: 1471 QLVKFTLESRRDEFFD---GFDNDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLG 1530
L + SR + F G S+P G++ Q E +
Sbjct: 1592 VLQAW--NSRLQDLFQQEKGRKRPSVPPS------------PGRLRQGEESKIN------ 1651
Query: 1531 GLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQPNNGTFEES 1590
+++ PR++ + E + + + + P E+
Sbjct: 1652 ----------AMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQV 1711
Query: 1591 SNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSA 1650
T+ L+ SG D V HLL S ++ S + +P ++
Sbjct: 1712 VGGPTD---LDSASGSED----------VFDGHLLGSTDSQVKEKSTMKAIFANLLPGNS 1771
Query: 1651 YYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIA 1710
Y P P D D ++ EH R+ + V ++EP+SIIA
Sbjct: 1772 YN--------PIPFPFDPDKHYL-------MYEHERV---------PIAVCEKEPSSIIA 1831
Query: 1711 YSLLMPEYNVQMSEPEKA------------------------------LEPGDTSRSL-- 1713
++L EY + E KA + G T+R++
Sbjct: 1832 FALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEA 1891
BLAST of Sed0006163 vs. ExPASy TrEMBL
Match:
A0A1S4DVR6 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103487265 PE=4 SV=1)
HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1397/1814 (77.01%), Postives = 1525/1814 (84.07%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENK+S+LVGL KSW+ WRS+ NV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKISDLVGLVKSWM-PWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+NFI A STD TN EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61 RCGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
S+SSSS+SM S+KS YTC SA+S+I STPCS S QYYDPYCS I DE D+LR+GK+INR
Sbjct: 121 SVSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S Y GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN D GLNL+
Sbjct: 181 TTSLMTSSSSYC--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLER 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
EN TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK++EPMDFQNNG+L
Sbjct: 241 ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKWAP-SFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
WLPPEPEDEED+K L F+DD+D +PG+W SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301 WLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360
Query: 361 GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
GHFRALV QLLEAE+LPIGD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361 GHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420
Query: 421 KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421 KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480
Query: 481 QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481 QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540
Query: 541 ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ K S KTLMFFEGCPKPLCCT+L
Sbjct: 541 ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600
Query: 601 LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNI 660
Query: 661 DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
DRSIS+IPGFTC P PE EIGQS KDAV ++D SII +NV NL+SLEP +
Sbjct: 661 DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLL 720
Query: 721 EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
E+N R+L+ +EYS+G MTIKE D D +SSSGK
Sbjct: 721 ESNATSTSFSFLKQDFSTMLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSGK 780
Query: 781 DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
EASS+ N I +S +SLD +TQ+ NDSTSQ PE+ + VTTKSSREDFPLSPSDQQN
Sbjct: 781 REASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQN 840
Query: 841 ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841 ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900
Query: 901 HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
HCYTHPQGSLTISAKKL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+
Sbjct: 901 HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960
Query: 961 ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961 LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020
Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
+EFH +NLEWINKEANEVHNRAKTLF EV KALPLIS +ESG EQEDFG +EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQEL 1080
Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
EQIINMEKE+FEVHFN+VL SG + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKVL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140
Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
VQRL+Y A LRKK+SPD SSNDI L+E+EL+SLE+LV+ TL S RDEFF G DNDSLP
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPP 1200
Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
ESK E LG G SG IS+ NE REKN DLGG EDD CLS PVNS P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLS-PVNSEPVDVGIGIRRANS 1260
Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
GEYPIM DLS TLDAVWKGVI PN+ FE+S S TNSA+LEPL+GQSDMEKC A G
Sbjct: 1261 VGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF-SRTNSAILEPLAGQSDMEKCTAN--G 1320
Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
+ETAHLLHSA+VLT DDSVETIT+ST+MP SAY +KISLLNIPK +ISDY FISS+RE
Sbjct: 1321 IETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380
Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEY QMSEP K +E DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSL 1440
Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
P+IGS K++S+KT DEEGS TILSMP+TE QFED V + ++ HTRV FV
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFV 1500
Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560
Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620
Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
+D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSG N VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKA 1680
Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740
Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1795
BLAST of Sed0006163 vs. ExPASy TrEMBL
Match:
A0A6J1EZX4 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438136 PE=4 SV=1)
HSP 1 Score: 2709.9 bits (7023), Expect = 0.0e+00
Identity = 1397/1798 (77.70%), Postives = 1527/1798 (84.93%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP +QDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+ KSINR
Sbjct: 121 SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS V+FD CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L DDD+DS +PG+W PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DSSII NNV NL+S L+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720
Query: 721 FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
+ + +Q+ SI + + DDDT+ SGK E SSQ N I IS
Sbjct: 721 ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780
Query: 781 IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
++LDG+TQASN STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT
Sbjct: 781 SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840
Query: 841 CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841 CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900
Query: 901 KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
KL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+ FSNNA
Sbjct: 901 KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960
Query: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020
Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
NEVHNRA+TLFAEV KALPLI E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080
Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
NEVL SGE +GQP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140
Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
P SSSND+ LDER ++S+E+ V+FTL +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200
Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
QISQ NE EREK+ DLG LEDDLCLSP +P+DVEIS+RRANSEG+YPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGDYPIMADLSDTLD 1260
Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
AVWKGV+ PN+ T +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320
Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
DSVET TS +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380
Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
+DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440
Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
EE + TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500
Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560
Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620
Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740
Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778
BLAST of Sed0006163 vs. ExPASy TrEMBL
Match:
A0A6J1KWW8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111497876 PE=4 SV=1)
HSP 1 Score: 2699.8 bits (6997), Expect = 0.0e+00
Identity = 1399/1799 (77.77%), Postives = 1521/1799 (84.55%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP LQDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
S SSSS+S+ S+KSDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+GKSINR
Sbjct: 121 SPSSSSTSIGSTKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC NADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDANADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS VIFD C R +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVIFDNQCQRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L FDDD+DS +PG+W PS SL GE P+KG SSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLFDDDDDSCAPGEWGYVPS-SLDIGECPVKGSSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKR ESIIVKGIICKKNVAH+RMTREISK R FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRCESIIVKGIICKKNVAHRRMTREISKPRFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K S KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKSTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV L HNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALLHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DS II NNV NL+S L+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSPIISNNVVNLNSLRLLKSN 720
Query: 721 FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
+ + +Q+ SI + + DDDT+ SGK E SSQ N I IS
Sbjct: 721 ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780
Query: 781 IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
++LDG+TQASN STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT
Sbjct: 781 SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840
Query: 841 CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841 CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900
Query: 901 KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
KL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+ FSNNA
Sbjct: 901 KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960
Query: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961 AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPALIEFYHNNLEWINKEA 1020
Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
NEVHNRA+TLFA V KALPLI +E G+EQED GVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAAVCKALPLIPKNELGSEQEDVGVNELNHYIQELEQIIIMEKEDFEVHF 1080
Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
NEVL SGE + QP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNS
Sbjct: 1081 NEVL-----------SGETTVSQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140
Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
P SSSND+ LDER ++S+E+ VKFTL +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVKFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200
Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSE-GEYPIMADLSDTL 1260
QISQ NEMEREKN DLG LEDDLCLSP +P+DVEIS+RRANSE GEYPIMADLSDTL
Sbjct: 1201 QISQLNEMEREKNIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGGEYPIMADLSDTL 1260
Query: 1261 DAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLT 1320
DAVWKGV+ PN+ T ESS S TNSA+LEPL QSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 DAVWKGVMHPNSVT-SESSYSQTNSAILEPLVVQSDMEKCNANLVGAETARLLHSALVLT 1320
Query: 1321 RDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVG 1380
DSVET TS +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVG
Sbjct: 1321 MVDSVETSTSLVSMPSSAYSTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVG 1380
Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTS 1440
S+DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT
Sbjct: 1381 SNDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTC 1440
Query: 1441 DEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITG 1500
DEE + TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT
Sbjct: 1441 DEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITC 1500
Query: 1501 FFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQ 1560
+FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+
Sbjct: 1501 YFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
Query: 1561 SFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHN 1620
SF QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+LKGGKECK+DILVMENLLFRHN
Sbjct: 1561 SFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKECKLDILVMENLLFRHN 1620
Query: 1621 VTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
VT+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
Query: 1681 LASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVI 1740
LASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740
Query: 1741 SPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
SPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKRYRYQSD 1779
BLAST of Sed0006163 vs. ExPASy TrEMBL
Match:
A0A0A0M3A5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_1G662800 PE=4 SV=1)
HSP 1 Score: 2697.5 bits (6991), Expect = 0.0e+00
Identity = 1387/1814 (76.46%), Postives = 1526/1814 (84.12%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENK+S+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1 MGHPENKISDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCS+CT+NFI APSTD+TN EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61 RCGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+SM S+KS YTC SA+S+I STPCS QYYDPYCS + DE D+LR+GK+INR
Sbjct: 121 SLSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN DEG NL+
Sbjct: 181 TTSLMTSSSSYY--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLER 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
EN TKGS V FDT PR NEE SSEDSDENDKGSECDFP+Y MQNKN+EPMDFQNNG+L
Sbjct: 241 ENTSTKGSPVNFDTQSPRGNEE-SSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKWA-PSFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
WLPPEPEDEED+K L F+DD+D +PG+W SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301 WLPPEPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360
Query: 361 GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
GHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361 GHFRALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420
Query: 421 KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421 KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480
Query: 481 QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481 QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540
Query: 541 ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ K S KTLMFFEGCPKPLCCT+L
Sbjct: 541 ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600
Query: 601 LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNV 660
Query: 661 DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
DRSIS+IPGFTC P PE EIGQS KDAV ++DSSII +NV NL+SLEP +
Sbjct: 661 DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLL 720
Query: 721 EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
E+N R+L +EYS+G M IKE G DDD +SSSGK
Sbjct: 721 ESNATSPSFSFLKQDFSTMISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSGK 780
Query: 781 DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
EASS+ N + ++ +SLD +TQ+ N+STSQ PE+ + VTTKSSREDFPLSPSDQQN
Sbjct: 781 REASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQN 840
Query: 841 ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841 ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900
Query: 901 HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
HCYTHPQGSLTISAKKL F EPEGKIWMWHRCLKCPRTNGFPPATRR+I+
Sbjct: 901 HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960
Query: 961 ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961 LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020
Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
+EFH +NLEWINKEANEVHNRAKTLF EV KALPLI +ESG EQEDFGV+EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQEL 1080
Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
EQIINMEKE+FEVHFN++L SG + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKLL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140
Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
VQRL+Y A LRKK+SPD SSNDI +L E+EL+SLE+LV+ TL S RDEFF G DNDSLP
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPA 1200
Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
ESK E LG G SG IS+ N+ +REKN DLGG EDD CLS PVN P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNKTDREKNIDLGGQEDDFCLS-PVNLDPVDVGIGIRRANS 1260
Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
GEYPIM + TLDAVWKGVI PN+ F++S S T SA+LEPL+GQSDMEKC A
Sbjct: 1261 VGEYPIMT-VPGTLDAVWKGVIHPNSLIFDDSF-SRTTSAILEPLAGQSDMEKCTAN--R 1320
Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
+ETAHLLHSA+VLTRDDSVETI +ST+MP SAY +KISLLNIPK +ISDY FISS+RE
Sbjct: 1321 IETAHLLHSALVLTRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380
Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYS+LMPEY QMSEP K +E DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSL 1440
Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
P+IGS K++S+KT DEEGS TILSMP+TE QFED V ++++ HTRV F+
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFI 1500
Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560
Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620
Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
+D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSGKN VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKA 1680
Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740
Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1794
BLAST of Sed0006163 vs. ExPASy TrEMBL
Match:
A0A6J1EUT4 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438136 PE=4 SV=1)
HSP 1 Score: 2696.8 bits (6989), Expect = 0.0e+00
Identity = 1390/1785 (77.87%), Postives = 1516/1785 (84.93%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
MGHPENKLS+LVGL KSW+ WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1 MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFCSKCT+N I APSTD TN EDSEKIRVCNYCFKQ G+EP +QDGDKAT SA+
Sbjct: 61 RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAV 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN QYYDPY S ITDEQD+LR+ KSINR
Sbjct: 121 SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180
Query: 181 TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
TT S+S YY G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181 TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240
Query: 241 ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
E+I KGS V+FD CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241 EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300
Query: 301 WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
WLPPEPEDEED+K L DDD+DS +PG+W PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301 WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360
Query: 361 EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
EGHFRALV QLLEAE+LPIGD P DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361 EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420
Query: 421 VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK FVLGGALEYQRV NH+SSLDTL
Sbjct: 421 VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480
Query: 481 MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
+QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481 LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540
Query: 541 GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541 GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600
Query: 601 LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
LLRGA DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP SP+TV LPHNPSN
Sbjct: 601 LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660
Query: 661 TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDSLEPEFIE 720
DRSIS+IPGFTCPSP+ ++ EIGQ KDAVPN+DSSII+ N+ +LD+
Sbjct: 661 IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIIKANI-SLDN------- 720
Query: 721 ANRNLERQEYSIGTMTIKEAVGADDDTISSSGKDEASSQRCNPISISIDSLDGSTQASND 780
DDDT+ SGK E SSQ N I IS ++LDG+TQASN
Sbjct: 721 ----------------------KDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNG 780
Query: 781 STSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYG 840
STSQRPES N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT CDRAHLLRIKYYG
Sbjct: 781 STSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYG 840
Query: 841 SFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLTLFY-REPEG 900
SFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAKKL F EPEG
Sbjct: 841 SFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEG 900
Query: 901 KIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLH 960
KIWMWHRCLKCPRTNGFPPATRR+I+ FSNNAAASRVAGCGHFLH
Sbjct: 901 KIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLH 960
Query: 961 RDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAE 1020
RDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEANEVHNRA+TLFAE
Sbjct: 961 RDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAE 1020
Query: 1021 VYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNE 1080
V KALPLI E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHFNEVL
Sbjct: 1021 VCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVL--------- 1080
Query: 1081 VPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDE 1140
SGE +GQP VDIFEINKLHMHILFLSY WVQRLSYA LR+KNSP SSSND+ LDE
Sbjct: 1081 --SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDE 1140
Query: 1141 RELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSGQISQSNEMEREKN 1200
R ++S+E+ V+FTL +D+FF G DNDSLPLESKHEIDLG GG SGQISQ NE EREK+
Sbjct: 1141 RGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKD 1200
Query: 1201 FDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQPNNGT 1260
DLG LEDDLCLSP +P+DVEIS+RRANSEG+YPIMADLSDTLDAVWKGV+ PN+ T
Sbjct: 1201 IDLGVLEDDLCLSP---VMPQDVEISMRRANSEGDYPIMADLSDTLDAVWKGVMHPNSAT 1260
Query: 1261 FEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAM 1320
+SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT DSVET TS +M
Sbjct: 1261 -SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSM 1320
Query: 1321 PSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPT 1380
PSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS+DTVVPVYDEEPT
Sbjct: 1321 PSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPT 1380
Query: 1381 SIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG-------- 1440
SIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT DEE +
Sbjct: 1381 SIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSK 1440
Query: 1441 --TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1500
TILSMP+ E QFED + Q+ HTRV FVNETPLGPVKYTIT +FAKRFEALRK+CC
Sbjct: 1441 EETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCC 1500
Query: 1501 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1560
PSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+SF QFA AYF+YLS
Sbjct: 1501 PSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLS 1560
Query: 1561 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1620
EA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNVT+LYDLKGSSRSR
Sbjct: 1561 EAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSR 1620
Query: 1621 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1680
YNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASI VMDYSLLVG
Sbjct: 1621 YNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVG 1680
Query: 1681 LDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1740
LDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVISPHQYKKRFRKAMT
Sbjct: 1681 LDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1735
Query: 1741 TYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
TYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK QSD
Sbjct: 1741 TYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1735
BLAST of Sed0006163 vs. TAIR 10
Match:
AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 865/1807 (47.87%), Postives = 1133/1807 (62.70%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
M ++K V + KSWI +S S N++RDFWMPD+SC VCYECD+QFT RRHHCR
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFC+KC +N I +PS ++ + E+ E+IRVCNYC+KQ + +G +S I
Sbjct: 61 LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SL SSS S + T S+ I+ST + +P S ++ DS +S + N
Sbjct: 121 SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180
Query: 181 TTPRTASTSGYYYD------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYC 240
+ R++ G+ D G G+ +DDD Y + + +Q +DYY
Sbjct: 181 KSRRSSDHYGHVLDSSDNQVEFFVNSGRSDGEADDDDDYQSDF-----AQSYAQGNDYYG 240
Query: 241 AVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECDF 300
A+N DE ++ G ++ K I P + + L++E D EN +
Sbjct: 241 AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300
Query: 301 PAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFSL 360
++ E +DF+++G+LWLPPEPE+EED++ + DDD D G W PS S
Sbjct: 301 SPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSF 360
Query: 361 GSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSW 420
++ K +SS K VVEGHFRALV QLLE ++LP+ + +GWLDIIT LSW
Sbjct: 361 NEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSW 420
Query: 421 EAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSR 480
EAAT+LKPD SKSG MDP GYVKVKC+ G+RSES++VKG++CKKNVAH+RMT +I K R
Sbjct: 421 EAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPR 480
Query: 481 IFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYL 540
+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEYL
Sbjct: 481 LLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYL 540
Query: 541 VAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQA 600
+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F Q
Sbjct: 541 LAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQV 600
Query: 601 SKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEG 660
+K AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY FAAYHLALET FLADEG
Sbjct: 601 AKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEG 660
Query: 661 ASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVPN 720
AS+ ELP ++P+TV LP PS +RSIS+IPGFT P+ EL E ++ D N
Sbjct: 661 ASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGN 720
Query: 721 KDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGKD 780
SS + + D ++P ER +++ T+ K E + + DD + + +
Sbjct: 721 FTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLES 780
Query: 781 EASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPLS 840
S S+ D L G+T+ D + N +SS+ DF S
Sbjct: 781 RQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPS 840
Query: 841 PSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSM 900
SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ C SC+M
Sbjct: 841 ASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTM 900
Query: 901 PSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII- 960
P+EAH+HCYTH QGSLTIS KKL L + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901 PAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMS 960
Query: 961 ---------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVL 1020
FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961 DAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVT 1020
Query: 1021 IPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSH 1080
+PP L F++ N EW+ KE+ EV +A+ LF EV +AL IS G + N++
Sbjct: 1021 LPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKL 1080
Query: 1081 YVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILF 1140
++EL ++ K++++ ++L+ V GQP +DI INKL I+F
Sbjct: 1081 SLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLIIF 1140
Query: 1141 LSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDN 1200
SY W + L+ AA + + N ++ N P + R + SLE+L ++S N
Sbjct: 1141 DSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AICN 1200
Query: 1201 DSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDVE 1260
DSL ++ +E L G S + D+G C P VE
Sbjct: 1201 DSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFVE 1260
Query: 1261 IS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQS 1320
S ++ A++E ++ DLSDTLDA W G NG F S + + + P
Sbjct: 1261 SSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP----- 1320
Query: 1321 DMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKPN 1380
+ L+G E S + + + T+ +PS ++Y +K LN K
Sbjct: 1321 -----DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHI 1380
Query: 1381 ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSE 1440
+++ ++SS+RELE + RLL+ +G +D V+PVYD+EPTSIIAY+L EY QMS
Sbjct: 1381 MAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSG 1440
Query: 1441 PEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNKH 1500
+K+ + D+ S + S L SL + + S+ + Q + + ++ H
Sbjct: 1441 SDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLH 1500
Query: 1501 TRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVF 1560
R+SF +E P G VKY++T ++AK FEALR CCPSE D++RSL RC++WGAQGGKSNVF
Sbjct: 1501 ARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVF 1560
Query: 1561 FAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLK 1620
FAK+LD+RFIIKQVTK EL+SFI+F AYF YL+E+ T PT LAK++GIYQVSSK+LK
Sbjct: 1561 FAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLK 1620
Query: 1621 GGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPI 1680
GGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSPI
Sbjct: 1621 GGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPI 1680
Query: 1681 FLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLE 1736
F+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHLE
Sbjct: 1681 FVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLE 1740
BLAST of Sed0006163 vs. TAIR 10
Match:
AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1133/1808 (62.67%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
M ++K V + KSWI +S S N++RDFWMPD+SC VCYECD+QFT RRHHCR
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFC+KC +N I +PS ++ + E+ E+IRVCNYC+KQ + +G +S I
Sbjct: 61 LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SL SSS S + T S+ I+ST + +P S ++ DS +S + N
Sbjct: 121 SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180
Query: 181 TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
+ R++ G+ D G G+ +DDD Y + + +Q +DYY
Sbjct: 181 KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240
Query: 241 CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
A+N DE ++ G ++ K I P + + L++E D EN +
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300
Query: 301 FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
++ E +DF+++G+LWLPPEPE+EED++ + DDD D G W PS S
Sbjct: 301 GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360
Query: 361 LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
++ K +SS K VVEGHFRALV QLLE ++LP+ + +GWLDIIT LS
Sbjct: 361 FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420
Query: 421 WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
WEAAT+LKPD SKSG MDP GYVKVKC+ G+RSES++VKG++CKKNVAH+RMT +I K
Sbjct: 421 WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480
Query: 481 RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481 RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540
Query: 541 LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F Q
Sbjct: 541 LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600
Query: 601 ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
+K AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY FAAYHLALET FLADE
Sbjct: 601 VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660
Query: 661 GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
GAS+ ELP ++P+TV LP PS +RSIS+IPGFT P+ EL E ++ D
Sbjct: 661 GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720
Query: 721 NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
N SS + + D ++P ER +++ T+ K E + + DD + + +
Sbjct: 721 NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780
Query: 781 DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
S S+ D L G+T+ D + N +SS+ DF
Sbjct: 781 SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840
Query: 841 SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ C SC+
Sbjct: 841 SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900
Query: 901 MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
MP+EAH+HCYTH QGSLTIS KKL L + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901 MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960
Query: 961 ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961 SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020
Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
+PP L F++ N EW+ KE+ EV +A+ LF EV +AL IS G + N++
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080
Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
++EL ++ K++++ ++L+ V GQP +DI INKL I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLII 1140
Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
F SY W + L+ AA + + N ++ N P + R + SLE+L ++S
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200
Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
NDSL ++ +E L G S + D+G C P V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260
Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
E S ++ A++E ++ DLSDTLDA W G NG F S + + + P
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320
Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
+ L+G E S + + + T+ +PS ++Y +K LN K
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380
Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
+++ ++SS+RELE + RLL+ +G +D V+PVYD+EPTSIIAY+L EY QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440
Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
+K+ + D+ S + S L SL + + S+ + Q + + ++
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500
Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
H R+SF +E P G VKY++T ++AK FEALR CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560
Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
FFAK+LD+RFIIKQVTK EL+SFI+F AYF YL+E+ T PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620
Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680
Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740
BLAST of Sed0006163 vs. TAIR 10
Match:
AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1476.5 bits (3821), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1130/1808 (62.50%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
M ++K V + KSWI +S S N++RDFWMPD+SC VCYECD+QFT RRHHCR
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
CGRVFC+KC +N I +PS ++ + E+ E+IRVCNYC+KQ + +G +S I
Sbjct: 61 LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120
Query: 121 SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
SL SSS S + T S+ I+ST + +P S ++ DS +S + N
Sbjct: 121 SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180
Query: 181 TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
+ R++ G+ D G G+ +DDD Y + + +Q +DYY
Sbjct: 181 KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240
Query: 241 CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
A+N DE ++ G ++ K I P + + L++E D EN +
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300
Query: 301 FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
++ E +DF+++G+LWLPPEPE+EED++ + DDD D G W PS S
Sbjct: 301 GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360
Query: 361 LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
++ K +SS K VVEGHFRALV QLLE ++LP+ + +GWLDIIT LS
Sbjct: 361 FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420
Query: 421 WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
WEAAT+LKPD SKSG MDP GYVKVKC+ G+RSES++VKG++CKKNVAH+RMT +I K
Sbjct: 421 WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480
Query: 481 RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481 RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540
Query: 541 LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F Q
Sbjct: 541 LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600
Query: 601 ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
+K AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY FAAYHLALET FLADE
Sbjct: 601 VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660
Query: 661 GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
GAS+ ELP ++P+TV LP PS +RSIS+IPGFT P+ EL E ++ D
Sbjct: 661 GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720
Query: 721 NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
N SS + + D ++P ER +++ T+ K E + + DD + + +
Sbjct: 721 NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780
Query: 781 DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
S S+ D L G+T+ D + N +SS+ DF
Sbjct: 781 SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840
Query: 841 SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ C SC+
Sbjct: 841 SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900
Query: 901 MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
MP+EAH+HCYTH QGSLTIS KKL L + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901 MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960
Query: 961 ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961 SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020
Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
+PP L F++ N EW+ KE+ EV +A+ LF EV +AL IS G + N++
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080
Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
++EL ++ K++++ N V GQP +DI INKL I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKQMLNVVKD----------------GQPTIDILLINKLRRLII 1140
Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
F SY W + L+ AA + + N ++ N P + R + SLE+L ++S
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200
Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
NDSL ++ +E L G S + D+G C P V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260
Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
E S ++ A++E ++ DLSDTLDA W G NG F S + + + P
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320
Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
+ L+G E S + + + T+ +PS ++Y +K LN K
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380
Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
+++ ++SS+RELE + RLL+ +G +D V+PVYD+EPTSIIAY+L EY QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440
Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
+K+ + D+ S + S L SL + + S+ + Q + + ++
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500
Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
H R+SF +E P G VKY++T ++AK FEALR CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560
Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
FFAK+LD+RFIIKQVTK EL+SFI+F AYF YL+E+ T PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620
Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680
Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740
BLAST of Sed0006163 vs. TAIR 10
Match:
AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )
HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 882/1830 (48.20%), Postives = 1150/1830 (62.84%), Query Frame = 0
Query: 9 SELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCS 68
SE+VGL KSW+ WRS V+RDFWMPD+SCRVCYECD QFT I RRHHCR CGRVFC
Sbjct: 12 SEIVGLIKSWL-PWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCG 71
Query: 69 KCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAISLSSSSS- 128
KCT+N I +D ED E+IRVCNYCF+Q + DG S+ LS+S S
Sbjct: 72 KCTANSIPFAPSDLRTPREDWERIRVCNYCFRQ-----WEQGDGGPHVSNITELSTSPSE 131
Query: 129 -SMDSSKSDYTCQSALSNINSTP-----CSNSHQYYDPYCSFPITDEQDSLRSGKSINRT 188
S+ SSK+ T S+ + S P H D + E + GK +R
Sbjct: 132 TSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRR 191
Query: 189 TPRTAS---TSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNL 248
+ A+ + R DDE D+ YG Y++D ET H ++++YY + + G+ +
Sbjct: 192 SSFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYN-GMGI 251
Query: 249 D--------GENICTK---GSAVIFDTPCPRSNEELSSED---SDENDKGSECD--FPAY 308
D GE K GS +I C S SE E+D EC+ PA
Sbjct: 252 DDVPCKHLGGETADQKSLSGSPLIHQ--CLESLIREGSEQFQKKSEHDGRDECEASSPAD 311
Query: 309 GMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK--LLFFDDDNDSRSPGKWA---PSFSLGS 368
++ EP+DF+NNG+LW+PPEPE+EED++ LF ++DN+ + G+W PS S GS
Sbjct: 312 ISDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGS 371
Query: 369 GEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEA 428
GEY + R++EE++K K VV+GHFRAL+ QLL+ E++ + D + WL+IIT LSWEA
Sbjct: 372 GEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEA 431
Query: 429 ATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIF 488
A +LKPDMSKSG MDP GYVKVKC+A G R +S++VKG++CKKNV ++RM+ +I K+R+
Sbjct: 432 ANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLL 491
Query: 489 VLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVA 548
+LGG LEYQRV N +SS DTL+QQE DHLKMAVAKI A PNILLVE+SVSR AQEYL+A
Sbjct: 492 ILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLA 551
Query: 549 KNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASK 608
K+I++VLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + GQ K
Sbjct: 552 KDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGK 611
Query: 609 TSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
KTLM+FE CPKPL T+LLRGA++DELKKVKHVVQY FAAYHLALET FLADEGAS
Sbjct: 612 KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671
Query: 669 VPELPSKSPLTVILPHNPSNTDRSISSIPGFTC----PSPELNTEIGQSRKDAVPNKDSS 728
PELP SP+TV LP ++ +RSIS++PGFT SP + + R ++VP +
Sbjct: 672 -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELL 731
Query: 729 IIRNNVANLDSLEP-------EFIEANRNLERQEYSIGTMTIKEAV--GADDDTISSSGK 788
N++ + P + E N + ++I ++ + + V + D S
Sbjct: 732 STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNI-SLNLPDRVIESRNSDLSGRSVP 791
Query: 789 DEASSQRCNPISISIDSLDGSTQAS-------------------NDSTSQRPESPKAN-- 848
+ + + NPI ++ ++ + S S D+ S+ + + N
Sbjct: 792 VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 851
Query: 849 -PVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLR 908
P T+S +E+FP SPSD Q+ILVSLS+R VWKGT C+R+HL RIKYYGSFD+PLGRFLR
Sbjct: 852 KPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLR 911
Query: 909 DNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLT-LFYREPEGKIWMWHRCLKC 968
D+LFDQSYRC SC MPSEAHVHCYTH QGSLTIS KKL L E EGKIWMWHRCL+C
Sbjct: 912 DHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRC 971
Query: 969 PRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGR 1028
PR NGFPPAT R+++ FSN+AAASRVA CGH LHRDCLRFYGFG
Sbjct: 972 PRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGN 1031
Query: 1029 TVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNS 1088
VACF Y+ I+V SV +PP IL F++ N +WI +E +EV RA+ LF+EV A+ I +
Sbjct: 1032 MVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI--A 1091
Query: 1089 ESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQP 1148
E G + + ELE+++ EK +FE + ++L E+ NE GQP
Sbjct: 1092 EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREV---NE--------GQP 1151
Query: 1149 RVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVK 1208
VDI E+ ++H +LF SY W RL A+ L K + D + +RE + L K
Sbjct: 1152 LVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDT--------KREENEKPPLAK 1211
Query: 1209 FTLESRRDEFFDGFDNDSLPLESKHEIDLG-NGGYSGQISQSNEMERE--KNFDLGGLED 1268
+ + + L + E++L +G +G N +++E N DL +D
Sbjct: 1212 -------SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKD 1271
Query: 1269 DLC-LSP----PVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQ-----PNN 1328
D +SP P S P + ++ +RR S+G+ +M +LS TLDA W G Q P N
Sbjct: 1272 DGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTN 1331
Query: 1329 GTFEESSNSGTNSAVLEPLSG---QSDM-EKCNATLVGVETAHLLHSAIVLTRDDSVETI 1388
++ +NS+ P+S D+ E+ N V + L S +DSV
Sbjct: 1332 NKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSV--- 1391
Query: 1389 TSSTAMPSSAYYSKIS---LLNIPK-PNISDYDHAFISSFRELEKQEHLRLLMHVGSSDT 1448
S ++P +Y I+ LL+ K ++ +ISSFRE E Q RLL+ VG +D
Sbjct: 1392 -SWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDI 1451
Query: 1449 VVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEG 1508
VVPVYD+EPTS+IAY+L+ PEY Q S ++L + ++P + S+
Sbjct: 1452 VVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNGSV 1511
Query: 1509 SGTILSMPKT-ERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1568
+ILS+ + D +S + H RVS+ + LG VKYT+T ++AKRFEALR C
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571
Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1628
PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK EL+SFI+FA AYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631
Query: 1629 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1688
E+ T PTCLAK++GIYQV++K LK GKE K+D+L+MENLLF V +LYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691
Query: 1689 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1733
YN D+SG N VLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLA VMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751
BLAST of Sed0006163 vs. TAIR 10
Match:
AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )
HSP 1 Score: 984.6 bits (2544), Expect = 1.1e-286
Identity = 651/1799 (36.19%), Postives = 958/1799 (53.25%), Query Frame = 0
Query: 1 MGHPENKLSELVGLAKSWISSWRSNS----ENVARDFWMPDRSCRVCYECDSQFTFIKRR 60
MG P+ L +L+ +SWI+S S+S + +DF + ++C++C T +++
Sbjct: 1 MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60
Query: 61 HHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYC---FKQLGREPFALQDGD 120
+ C CG +C C+ + K+++C C ++L + +
Sbjct: 61 YCCLSCGSCWCKSCSDT--------------EESKMKLCRECDAEVRELRVKSYDKVHPR 120
Query: 121 KATSSAISLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLR 180
+ SL++ S S+ SS C+ N+ S C S +E+++
Sbjct: 121 DSPDPPSSLATESESLASSLEIRDCR----NMASIRCYPSR-----------GEEEEARY 180
Query: 181 SGKSINRTTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVN-- 240
GK + + S G E + S + H S + + +
Sbjct: 181 CGKQLLSPSSDNYQDSSDIESGSVSARHE----LFSCKSSAGSSPHDSPLRNNFSPLGRF 240
Query: 241 ADEGLNLDGENICT---KGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKN 300
+L +C+ ++ D L ED +E ++K
Sbjct: 241 VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------EDKL 300
Query: 301 NEPMDFQNNGVLWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG---SGEYPMKG 360
+P+DF+NNG +W PP PEDE DD + +F D++ G A FSL S P K
Sbjct: 301 QQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIPTKE 360
Query: 361 RSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPD 420
+ E + + VV HFRALV +LL E+L D S WLDI+T L+W+AA +KPD
Sbjct: 361 KLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPD 420
Query: 421 MSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALE 480
G MDP YVK+KCVA G ++ESI+++GI+C KN+ HKRM + R+ +L G+LE
Sbjct: 421 TRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLE 480
Query: 481 YQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVL 540
YQRV ++S +TL+QQE +H+K +AKI++ PN+LLVE+S S +AQ+YL+ K I++VL
Sbjct: 481 YQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVL 540
Query: 541 NIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVE-TFEVHDTGDGQASKTSAKTL 600
N+KRSLL+RIA CTGA + P++D +++ +LG+C+ FR E E H+ G+ Q+++ ++TL
Sbjct: 541 NVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTL 600
Query: 601 MFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPS 660
M+FEGCP+ L CTV+LRG+ ++ELKKVKHV+QYA FAAYHL+LET FLADEGAS+P++
Sbjct: 601 MYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRL 660
Query: 661 KSPLTV--------------ILPHNPSNTD-----RSISSIPGFTCPSPELNTEIGQSR- 720
K P V ++ +P+ TD + + T P PE E+ +S
Sbjct: 661 KQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPE--HEVCESLC 720
Query: 721 KDAVPNK---DSSIIRNNVANLDSLEPEFIEANRNLERQEYSIGTMT-IKEAVGADDDTI 780
+D P + SS + + D+L +F NL + YS + + E I
Sbjct: 721 EDFDPTQIFPPSSEVETEQS--DTLNGDFA---NNLVTRSYSSNQLNDLHEPTLCLSSEI 780
Query: 781 SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
+ + S + N G + N + + + P+ ++ +
Sbjct: 781 PETPTQQPSGEEDN----------GRGEEENQLVNPQ-DLPQHESFYEDDVSSEYFSAAD 840
Query: 841 DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
Q+ILVS S+RCV K + C+R+ LLRIK+YGSFD+PLGR+L+D+LFD++ C SC
Sbjct: 841 SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900
Query: 901 EAHVHCYTHPQGSLTISAKKLLTL-FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
+AHV CY+H G+LTI+ ++L ++ E +GKIWMWHRCL+C +G PPATRR+++
Sbjct: 901 DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960
Query: 961 --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
FSN+A A+RVA CGH L RDCLRFYGFG VA F YS IN+ +VL+
Sbjct: 961 AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020
Query: 1021 PPPILEF--HHNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNEL-S 1080
PP +LEF H EWI EA E+ + +T++ E+ L + S E E +L S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080
Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
+ ++Q++ EK++++ + + + Q +DI E+N+L ++
Sbjct: 1081 RIIGLIDQLVK-EKDEYDDALQPIFEENLQI------------QGSLDILELNRLRRALM 1140
Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
++ W +L + KK S + +D R E D
Sbjct: 1141 IGAHAWDHQLYLLNSQLKKASVFKTGDD-------------------NAPRNPEMHDPPK 1200
Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEIS 1260
D E E D + + + E N D N P ++
Sbjct: 1201 IDRRMQEGSDERD-----------EQSHTDSEANGD--------------NKDPENIP-- 1260
Query: 1261 IRRANSEGEYPIMADLSDTLDAVWKGVIQ--PNNGTFEESSNSGTNSAVLEPLSGQSDME 1320
S G LS+ +D+ W G Q T E+ ++ L L+ ++
Sbjct: 1261 -----SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQ 1320
Query: 1321 KCNATL-------VGVETAHLLHSAI-VLTRDDSVETITSSTAMPSSAYYSKISLLNIPK 1380
++ + G+ + L S + + YS++ L + K
Sbjct: 1321 SFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQK 1380
Query: 1381 PN-ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQ 1440
+ I +ISS ++ + L+ G +D VVPVYD++P S+++Y++ EY
Sbjct: 1381 LDLIVGSAPTYISSASQMADGARM-LIPQRGLNDIVVPVYDDDPASVVSYAINSKEYK-- 1440
Query: 1441 MSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG----TILSMPKTERQF------- 1500
E + G + S+ S+L + E S LSM Q
Sbjct: 1441 ----EWIVNKG-------LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQ 1500
Query: 1501 EDRVS--IAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRC 1560
+DR S + + R S + G VK+++T +FA +F+ LRK CCPSE+D+VRSLSRC
Sbjct: 1501 DDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRC 1560
Query: 1561 KRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAK 1620
+RW AQGGKSNV+FAK+LDERFIIKQV K EL SF FA YF YL E+ + PTCLAK
Sbjct: 1561 QRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAK 1620
Query: 1621 VVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLL 1680
++GIYQVS K+ KGGKE K+D++VMENL + ++++YDLKGS+RSRYN +TSG + VLL
Sbjct: 1621 ILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLL 1648
Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGII 1715
D NL+E++ T PIFLGSKAKR LERA+WNDT+FLAS+ VMDYSLLVG DEE++ELV+GII
Sbjct: 1681 DMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGII 1648
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038877586.1 | 0.0e+00 | 77.33 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Benincasa his... | [more] |
KAG7020853.1 | 0.0e+00 | 77.81 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B [Cucurbita argyrosperma subsp.... | [more] |
XP_023520487.1 | 0.0e+00 | 77.92 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pep... | [more] |
KAG6588292.1 | 0.0e+00 | 77.81 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B, partial [Cucurbita argyrosper... | [more] |
XP_016899810.1 | 0.0e+00 | 77.01 | PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1... | [more] |
Match Name | E-value | Identity | Description | |
Q0WUR5 | 0.0e+00 | 47.84 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=370... | [more] |
Q9LUM0 | 0.0e+00 | 48.20 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=370... | [more] |
Q9SSJ8 | 1.5e-285 | 36.19 | Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thalia... | [more] |
Q9XID0 | 7.1e-179 | 32.04 | Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thalia... | [more] |
Q9Z1T6 | 9.5e-99 | 23.48 | 1-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve ... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S4DVR6 | 0.0e+00 | 77.01 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103487... | [more] |
A0A6J1EZX4 | 0.0e+00 | 77.70 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
A0A6J1KWW8 | 0.0e+00 | 77.77 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
A0A0A0M3A5 | 0.0e+00 | 76.46 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_1G... | [more] |
A0A6J1EUT4 | 0.0e+00 | 77.87 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
Match Name | E-value | Identity | Description | |
AT4G33240.2 | 0.0e+00 | 47.87 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |
AT4G33240.1 | 0.0e+00 | 47.84 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |
AT4G33240.3 | 0.0e+00 | 47.84 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |
AT3G14270.1 | 0.0e+00 | 48.20 | phosphatidylinositol-4-phosphate 5-kinase family protein | [more] |
AT1G71010.1 | 1.1e-286 | 36.19 | FORMS APLOID AND BINUCLEATE CELLS 1C | [more] |