Sed0006163 (gene) Chayote v1

Overview
NameSed0006163
Typegene
OrganismSechium edule (Chayote v1)
Description1-phosphatidylinositol-3-phosphate 5-kinase
LocationLG08: 25545913 .. 25566747 (-)
RNA-Seq ExpressionSed0006163
SyntenySed0006163
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAGAAAGTACACAACAGAATGGAGAGAGAAACTAGAGAGAGAAATTAGAGTCGCCATCGCCATCGCCATTACCAGATTTCCAGAACGTGATCGAACGATCATCAACGGAGTTCAGGCAAGACGACGGCGGCGAAGAGCGCGAAACACCGCCTTGATTCCATTTCTACAAGTTCTTGAGGTACGTCTAACAAATTCCGAACCAGCACCGTCAAATCATCCAAGCCGTGTGCCTCTTTCGTCCAGACTCATCGATTGAGGTTCGCATTTCCTTCTCTTTTCTTCCTACGAATAATGCGATGCTATTCGATTACGATGATCCTCATCTCCTTTGGATTGCTCTGTTTTTGGTATTTCTATGGCGTAATCATCTCTAGGTTTTTGCATTCCCCTTGAATCTTAACGTTGTTGTCTCCTTTATCGTTGTTCTTCTAGTTTTTGGATTCTTCTTTTCAGAATATGCCTTCATTCCGATAATCTATCTGCGTTCTTCTCTTATTGCTGGCTGTTATTCAATTGGAGTCTTAATTGTTTTTGCTATGGTGATGAGTGCTATTGCGTCTCTTCCTTGTCTTTGCGTATCAGATTTGGGCGGAATGAATGTTTCGTTGCGTCATGTTCGCTGGGTTCACTCGCCTGGAAAAGAAGAGCGTGTTTGATTTTGGTATTCTTTTTTTTTTTTTTTTTTTTGTCGCAATTTTAGGATTTTTGGTATGTTTCGTTGGTATTTTTATAAAATTTCGTGATCTCGTTCCTTAATTTTTCATTCTTTTTAAAACTAACACTTTTTTTTTTCTTTTTTCTTTTCAACAGGTTGATTAATTTAAAAATGTCTAAATCAGTTCTTATACGTGAGAGGTGGAAGTGATTGTTTCGTATTGCAAATGTGGGTGAGAGTTATTTATTAGTTTGTTTTTATTATAATTATAATATCTTAATTAGACTATGCATGAATTGGCATATTTGTTTTTTTGAAAAGCATGGATTGACATATTAATATACTTTAAAGAGAAAAAAGAATCACAAGAGGTAAAAGTTGGAAAAGATAAATTAGAAAGAGAGAGTTGGTTTAGTGTGAGAAAAAAAAACTCAATGAAAAGTTAGGGTGGTTAAATATCATAGAGCTTTGTCAAAAAAATTTAAATAGGCTATATGCCAAAGAATGAATAATCATATAGGTATATGTTAGGAAAATAAATTTTCAAGAGTGAGAGATAAATGTTAGGGAGAAGGTTTTTAAAGGAGTTCACAAAAAGAAAGAGAGAAAAATCTTTCAGAAAGTATACAAGTCTTTCAGAAAAAAGGAAAGGAGAATTGTAAGATTAAATGAAATGATCATCGAAGACTTAAATGTCTAATCTTTAAGGTACATTTATGATCCTTCAATTAGATCATCAATGTTTAACTTTGATAATCAAAATTGATGTATCTATTGAATTCAAAATTAATATTATAGCTAAATAAATTAGATCTCTACTATAACTTTTTCTTTTTATAACATACCTATTATCACTCTAAATGTTGATAAATCAGTCCTCTACTTTGCTATGTGCAGAATTTCGTAACCAGTGATTTCATATTAGATCGAGAAGGTTATTTTTTTATATATAAAAGAAAATAGAGAAGGTAATCAGGTGGTTGATGAGCAGATTGGGATGAGTGGAGTATCCACATCATCATCAACCTCATCAAATGGGCCATCCTGAAAACAAATTATCCGAACTTGTGGGCTTGGCAAAGTCATGGATATCATCATGGAGGTCTAACTCGGAAAATGTCGCGAGGGACTTTTGGATGCCTGACAGAAGTTGTAGAGTATGCTATGAATGTGACTCTCAATTCACATTTATCAAACGGCGGCATCATTGCCGTTTCTGTGGACGAGTTTTCTGTTCTAAATGTACTTCAAACTTCATTCGTGCTCCATCCACCGATTCAACGAATTGTATGGAGGATTCTGAGAAGATCAGAGTTTGTAATTATTGTTTTAAGCAGTTGGGGAGGGAGCCATTTGCCTTGCAAGATGGGGACAAAGCTACCAGCTCTGCTATTAGTCTATCTTCTTCATCGTCCAGCATGGATAGCAGTAAGTCCGATTACACCTGTCAAAGTGCACTCAGCAATATTAATTCAACGCCTTGCTCAAATAGTCATCAATATTATGACCCGTATTGTTCGTTTCCTATCACTGATGAGCAAGATAGTTTAAGAAGTGGGAAGAGCATCAATCGGACTACACCTCGCACGGCTTCAACATCTGGTTATTATTATGATGGTTATTACAGGTATTCTCAAGTGTTGATATTCGAGTTCTGGCTCATTTCCCCAACTTAGAAGAACTCCATTTGGGTCCTTGAATTTTCAAAAGGGCTTATTTAAGTGGTGAAATTTAAAATATATGCACTGAAATATTAATATAAATATGATTTTATGTCATCCCAAACCCCACCCTACCCCATATTGAGCTAAAAAAACATGGTGCGAAATCTCCCTCTTAATTTGTGCGAAAGATGATGCCACTGGGTCTTCGTTATGTTAATTCTATTGAAACTCACTTATGCCTTTTCAGTCATTTTCTTATGCAATATGCATGCGTTAGCCGATACTTGTCTTCAATTGTGAACACAGACAATGATACCATTTTTCTCTTATTTTACTGAACTTGTGTCTGTATTCCGGTTATCATTAATTGTTAATGCTTTTATACATTCGTTATTTATTTACTGTTGTATTTTCCTATTAGGGGTGATGATGAGGATGATGATTATTATTATGGAACGTATCGCTCAGATTCAGAAACCAAGCATATATCCCAGTCTGATGATTATTATTGTGCGGTAAACGCCGACGAGGGCTTAAATCTTGATGGAGAAAACATCTGTACTAAGGGTTCAGCAGTGATTTTTGACACTCCGTGTCCTAGGAGTAATGAGGAATTATCTAGTGAAGACTCTGATGAAAATGATAAAGGCTCTGAATGTGACTTTCCTGCTTATGGCATGCAAAATAAGAATAATGAACCTATGGATTTTCAGAACAATGGGGTTCTCTGGCTTCCTCCAGAGCCAGAAGATGAGGAAGATGATAAACTTCTTTTTTTTGATGATGATAATGATTCCCGTTCTCCAGGTAAATGGGCACCTTCTTTTTCCTTGGGCAGTGGGGAATACCCCATGAAGGGAAGGTCAAGTGAGGAGTACAGGAAAACTGCAAAATACGTCGTAGAAGGGCATTTTAGAGCTCTCGTTGGTCAACTTTTGGAGGCTGAGGATCTTCCTATAGGTGATGGTCCCAGTGGAGATGGCTGGTTAGATATTATAACATATCTGTCCTGGGAAGCAGCTACAGTTCTGAAGCCAGATATGAGCAAGAGTGGACCTATGGATCCTAGTGGATATGTTAAGGTTAAATGCGTTGCTGGTGGAAAACGTAGTGAGAGGTAAGGTAACTCCATGGATCAATAACGGTCCAGGTATATTATATTGATGTATGAATGTCAATTGCTTGCTTGAAGATTGACTCAATATTTGACAGAAGGATAAGGTTTATATTCTAGCAAGTTGGTGTCTATCTTATAAAGGATTATAGAATTCTTTTTCTTATGGGGCTGTATTACTCCCATGATTCTTTTTCCTTAGTTTCTTTTTCTTTTTTCACTCTTTATATGAGTTTGTTTCCTTTGAACATTTGATGTATGTTTTCATTTTCTACATGAAAATCTGTTTCTTGTTAAAAGAAAGATCGAGATCATTGGTGAATCACACTACTTTATGCACGTGATGACTATGTTTTTGAGATGAGGAGTTAGTCTGCTCTTGTGGTGTTTTTTTTTTAATTTTGAACTGGTAGTGCTAAAAGAACTCTTCATATGTTTTCTTAATTTCTATGTACATTCAGGGAGAATGTATTATGAGATTTTAGAGAACCAGTTGGTATGAAATGATTCCTTATTTTGAAATGTTGTCTCAAGATTCAACCTTAGTACCAAATTTCCAATGCATTTTTTTTTACCACGTTGTATGAGTATGACTGCATGTTACGTGTTTCTTTCTCCATTTGAGTGGTTATTGAGTTACTGCAGGGGTTTTTGGTTTGACATGACTATAATTCTTTTGCAGCATTATTGTTAAAGGCATCATTTGCAAGAAGAATGTTGCACACAAGCGGATGACGAGGGAAATATCTAAATCACGCATTTTTGTCCTTGGGGGAGCTTTGGAGTATCAACGTGTTATTAATCACGTGTCAAGTCTTGATACTTTGATGCAGCAGGTTTCGAACTATTTTCCCTTATCCTATTCATTAGGTCAATAATAATGAAGTTATGAAACTTGGTATACTCCGGTGCTTCATGGGTTCCTTGTATCTACATCCAGGAACTTGACCATTTGAAGATGGCTGTTGCTAAGATTGATGCCCATCATCCTAATATTCTTTTGGTAGAGAGATCAGTGTCTCGCCATGCTCAGGAGTACCTTGTCGCAAAAAACATCACTGTTGTTCTAAATATTAAACGATCACTCTTAGAGCGTATTGCTTCCTGCACTGGGGCAAGTATAATTCCTACTGTTGATCATGTTGCCTCGCTGAAACTTGGTTATTGTGATGCATTTCGTGTGGAGACATTTGAAGTACATGACACTGGTGATGGCCAAGCTAGCAAGACATCAGCGAAGACTTTGATGTTCTTTGAGGGATGTCCAAAGCCTCTATGTTGTACTGTAAGTCCGCTATCAACATGGAGAAATATGATTCAAAATGATAATAAATGATATAAGCATTTATTTCCTATGAGAGAAATATATTGTCTAGATAGTTAACGTATTTTTCATTTTGCTTGTCAGTGTAATTGAGTCGTATCAGAAATAAAGGGATTTCAATGATTTCTGACACTTCTCTAAAAACTATCTTGTTGTAGTTGTAACTCCTGAATTATACACGTTATAATAGATAATAGATTCTCATCTTTAAATGTCTAGTGGAAAAACCGGACATTGGTATTACTTGTCTTCATCTTGGGACATAGTTCTAAAGATTAAGTAAATTTGGTTTTTATAGATGGGATACCGCAATAGATGGTAATATTTCTAGTGTTGTCTTCAACTATAGGATGTGCCATATTGGCATGGAAATACTTATCTAAAGTAGACTTCTAATATAGAACTAACCTATACTTTCCTCTGCAACGTTACCTGAACAAACATATTGCATATAACTATGAGAAATAATAGACTAATTTTTTTTTGTCTTCAGGTTTTGCTAAGGGGTGCCAGTAAAGATGAGCTTAAAAAAGTTAAACATGTTGTTCAATATGCAACTTTTGCTGCCTATCACCTGGCTCTAGAAACATGCTTTCTGGCTGATGAAGGCGCTTCAGTTCCAGAGCTCCCATCGAAGTCCCCATTAACAGTTATTCTTCCTCATAATCCATCAAATACAGACAGGTCCATCTCCTCTATTCCTGGCTTTACTTGCCCATCACCTGAACTTAACACGGAAATCGGGCAATCTAGAAAAGATGCTGTTCCAAACAAGGACTCATCTATAATTAGGAATAATGTTGCCAATTTGGACTCTTTAGAGCCTGAATTTATTGAGGCAAATAGAAATCTGGAACGCCAGGAATATTCAATTGGCACGATGACTATCAAGGAAGCAGTAGGGGCAGATGATGATACAATTTCTAGTTCTGGTAAAGATGAAGCTTCGAGTCAACGTTGTAACCCTATCTCTATAAGCATCGATTCGTTGGATGGAAGTACACAAGCTTCTAATGATTCAACATCTCAAAGACCTGAAAGCCCGAAGGCTAATCCAGTGACCACCAAATCTTCAAGGGAAGATTTCCCTTTGTCACCTTCTGACCAACAAAACATTTTGGTTTCTTTGTCTACGCGTTGTGTGTGGAAGGGAACTACTTGTGATCGTGCTCATCTCTTACGAATTAAATACTATGGGAGCTTTGACGAACCCTTGGGTCGGTTTCTACGAGATAATTTGTTTGATCAGGTAATATAGTTTTCTAATGATTATTAACTATGGGAAGGTTCTTCTTTTGTATTATTCCTTTAGCCTCTTTCTGTGGGGAGTTTATCTCTGCCTCTATCCTTCGGGATGTCTATCTTTTTTTGAGGAATATGCTTATCTTTTTCTTATACATGAAACAAAGGATCAAGTAACAAAAGAATTTGTTTTTTTAGTTCAACAGTGGGGAATTCTAACTCATGACCTCTTAGCCATGAACATACCTATATCGGTCGACTCTGGCAAAAATATAATTTTCTTATCCAAAGCATTACCATTCATCTTCTTGCAGAGTTATCGATGTCATTCATGTAGTATGCCCTCCGAGGCACATGTGCATTGTTATACGCATCCTCAAGGCAGTCTTACAATATCTGCTAAGAAACTCCTGACTTTGTTCTACCGGGAACCTGAAGGTAAAATTTGGATGTGGCATCGGTGTTTGAAGTGCCCTCGGACCAATGGCTTCCCACCAGCCACTAGGAGAATTATTATGTCTGATGCTGCCTGGGGTTTATCTTTCGGTAAATTTTTTGGAGTTAAGCTTTTCAAATAATGCAGCTGCCAGCCGTGTTGCAGGTTGTGGCCATTTCCTTCATAGAGACTGTCTCCGTTTCTATGGGTATGATTTTCTTCCCATATAATTTTATTTCTCCAGAAATTAATATTGATTCTCACTGGTAAGTTCTGAGCATGACAGGAAATCAACTAGTTTACTTTATATCTGATTTTTAATTTCCTAAATGATGTCTATGCTTTTTTATATTTATAATTCAACATTGATATGGCATTGGCACAACTCTACAACTTTGAAAGGTTGAGCGAGATGCTTTTTTGGACGGTTGGATTAAACTTGTTACTGCTTTACTATCATCATCCTAGAAGTAACAAGATTTTAGGTATTTTTTTTATTTGAGACAATAAATTCAGTACATTATTCCTCAATTGTCATAGAAGTGTTGCCACTCAAAAGTAAAGTTACAAAGCTTTTATGAAAAAAAAAGTCCTTTTTATCAATGCTTAAGTGCTTGGAAATTGGAAAAGTTGTAAATTCTAGTTTGAAAAACTCAAGGCATGTACTTCTGGATCTTAAATGTCTATATTTTTTTTGCCTCTAATTCCAGTTCTTTGGATCCTGGACGAGTACCACATTTTTTGGTGGTGGCAGGTGTCTGGTTCTTGTAAATGTTTCTCAAATTGTTTTTGCATAGTAGTCCCTAAGGGTGGCACAATGGTTGAAGGCTTGGGTTTTGAGGGTGTGCTCCCCTCAAGGTTCTAGGTTCAAGACTAATATGTGGCATTAATTTGTAGATGCCCGTGCTCCATTTCTTCGATGTCTCCTGGTACCTGGCTTAGGGATGGGCATGGTTACCTTGGGTATAGTGAAGTGAAGTTCCGATTTTCCGGATTCAAAAAAAAAAAAAAGAAAATTGTTTTTGCAGAGTAAGGAGCCAATGCTCTCTTTGTTAATCTATTTAGAAAGCACAAGTTCCAAATTTATCTCTTGGTGGGCTGTTGAAATGTCATCATAGAAATCCTTAAAAAATCAATTTGAGCTTGAAAGATGCATATTTCTGAAAATGTACAGAATATATTGACCACCTATCTGACCGTTCCTCTCGTATGAAAATTTAGAAGACTTCTCTCAATTACAATGGAGCTTTCCCATTTTCTTTTGTGTTTAATTTATATTTGGGACATTTGCAATCTCGAATAACTGTGTATAAGTCTCCTGCCGACAACTATGCTCCGATTCCTTTCACTCTTGGGCTGATTTTAAGATCAGTCATCAAACCTTTTGAGTTTGAAAATATGATAATAATTTGCCTAAGATTGGCAGATGACAACTGGTGGAGATTTTCATCTAAAAATTGAGGAATGGTTTAATCAACTTCATGGTCAACCAGAATATATTGAAGAATATAAGGATTGGATTTCAATTGAGAATCTACCTCTCAATTTTTGGAAAAAGTGTGTTTTTGATGTCATTGGTCATTACTTTGGTAGTTTGGAGAATATTTCTTTTGCAACATTGAAGTTCATAAATTCATCAGCGGCTCGGCTAAAAGTGAAAAAAGTTCTTTATGGTTTCCTCCCAGCCACAATCTCGATATTTTTAGGATAAGACTTTTGGTAACTTTTCTTTGAAGTTTGTGGATATTGATTCCTAAGATCGTCTTGGTTTGGTTTTTAAGTCCCTCATTTTAATTTAATTTTTTTTCCAAATTGGGTAGATTTGATTCAATTTCGTTAAATTTTTTGAAATAGGGAATAAAATACATTCATTAAGAAAATGAGAAGAAAAGTACAAAGTACATGATACAAAAAGACATAACAAAAGAGGAAAAGCCTCAATAGACATAAGCAAGAAAACAGTTTGAAAGAAATGCGGAACTTATTAAAACAATCTTAGAAACATTGAGCTTGAAAAAGAAAGCTGAGATGATAAAAATAACTTGAATGGAAAAATTTGCAAAGGCCTTTGAGGAAGTCGCTCAAATAGAAATCTTCAAACAGGCTGATTCAATCCTGGAAGACGAAGGGATTTTTTAAGCATTTTTTTCTAGTAGCTGAGGTTGATCACAATCCCCTTTCCATCTTCTTTACCAGCCGATATTAATGTCTCGTGAAAGGGTAGTATTCAAGAAGGTTCTTATGATTTACCTTTATTAAATCTATCAAAATGTCGCTCAACATTTCAAGGGATTGCCTTTTCCGGTGCTTTTTCGAATTCTATTTTTCCTCAAGAGTTGCCAGCTTCGGTTATTCCAAATGATCCAAGGGATTGCCTTCTGCAATAGAAAATGATATTGTCTCAGAGAAAGAAGCAGCTCCTATTCTTCCACCGTCTATAAATTTCCATTCTTCTCTAAAGGAAATTAGAGATTGTTCTGCATTTCTTATTTTAAGTATTACACAATGAAGAAAGATAATGAATTATTGATTTCTTAACCAATCCATCCCCCAAATTTTAGCCTCTCATCAACCTACCCCACACTCTTCCACCTCTATCTGTTATAAATTTGACCACCTTCCTCTTTCTCAAGTTTGATTTTTCTTCGATTAGAGGGATTGTTGTCCATCAACCACTAACCCCTCTAAAGATATAGATCACCCCCTTTTGCAGATTTAGATATTGAAGATGGTTGGAGGGTTAGCAGTTTTGACTCTCCATGCAATCATCTTAAGGACGACATTTCCATCCTTTTGATGAAATCATACCCTTTTCAGCTATGTTTGATCCATCCAAGTGTTCTCCCGTGCCTCAAATTCCTGGTCAACATTTTGCATCTCTAGTGGTTTTCAATTGAATGTTATTTCCCCTAAATAAAGTAGCAGTAAACATCAAACATAGGGACGATATAGTGTTTCTACAATGAGTTTCACCATTATTGGATAAAGCACGACTTCTAGTAGGAGCTCAATTTGATTTGTTGTTTTGGTAGCTAACTCTACCCTTGATTTCTGCCCTTATAACGCGTTGATCTTTTCTGTTGTTATATCTCGCTGCTTTCAGATATTCAAATGCTCAAATTGTGAATTTCTCTTAAAATTTTAGCTCTTAGAATTACATTTATGTAGTAATGTTTTGTCTTTTCTATGCTTAGCGTATTTTTAGTCCTTAGGAGTTGTTCTTGGCTTTAGATTGCAAATCCTTACATTTTCCTTTCTTCTATTCTTTTTTGGGAGTTGTTTATAAAGCTACTGCTTTTTTTCATTTTTTCAATGAAAAGTTTGTTTTTCTGTTAAAAAATGAAATGTGCCATCTGTTCTCTTAATGCCGACTATTTTGAGGAATCCATAAAAAAGATTCTTCTCCTATGTGAAGTTTCAAATGTTGATGTTGGTGTTCTCGTAAACAGATCACCCCATTTTGCAAATTCAGATATTGAAGATGGCTCGAGTGTTAGCAGCTTTGACTCTTCATGCAATCATCTTAAGGATGACATTTCCCTCCCTTTTGCTGAAATCATATCCCCTTCAATTGTGTTTGATCCATCCAAGTGTTCTCGTGTGGCTCAAATTCCTGGTCAACATTTTGTATCTCTAGTGGAAGTTGTTGTATTTTAATATGAGAAAAGAATCACATTACCTCAATCATTAGAGAGACTATATATATATAGGCTCAAGGAAACCCTACCTAGGAATGGGAAAAGACTATAAAGGACAACACTTAAAACTAACTACTAAATAACCTCTAACACTCCCACTCAAGCTGGAGCATATATGTTAATCATGCCCAGCTTGTTACAAAGATAATCCACTCGCACTCCATTTAATGCTTTCGTAAAAATGTCTCCTTACTGGTCTTCTGTCTTCACATACGCATGTTGTATTTTCTCACGAACAAAAATCAATCTCAATATGCTTAGTCTGCTCATGAAACACAGGATTTGATGCGATATGGATAGCCGCTTGATTATCACCCCATAATTTGATCGAAGTCGGGTTATCAAAGCCTGATGCAACCATGAGTTGACATATCCACATTACTTCACATACTGATTGTGCCATAGCTCTATACTCTGACTTAGCACTTGATCGTGACACTACATTTTGTTTCTTACTTTTCCACGAAATCTGGTTACCTCCCACGAAAACACAATATCCAGAGGTCGATCTTCTATCGTCTCTTGCACATGCCCAATCAGCGTCTGAAAAACACTCCATTCTCATATGGACATGATCTTTATACAAGATCCCATGCCCGGGTGCAGCCTTCAAGTAACCCAAGATTTGTCGTACCGCTGTCCAATGATCCACGGTGGGTGAAGACATAAACTGACTCACAATGCTTACTGAATAAGCTATATCTGGTCGTGTCACAGTAAGACAATTCAACTTCCCAACTAACCTTCTATACCTTTTAGGATCGTCAAAAGGTTCTCCTTCTCTTGTGAATTACATATTAGGCATCATCGGAGTATTGCACGATTTGGCTCCCAATTTTCCTATTTTAGATAGTAAGTCAAGCACATATTTCCTCTGTGATAGGAAAATACCCTTCTTGTTCCTCAATACCCCAATTCCCAAGAAATATTTAAGCACACCTAAATCTTTAATGTGAAACTAATTATGTAGAAACGACTTAAGGGATAATATATCAGACATCACTCCTAGTAATCACAATATCGTCAACATATGCTACGAGTAAGATAACATCATTCGTAGATCGGCGATAGAAGACAGAATGGTCTGACTTTCTTTTCTGCATTCCAAATTGTTCAACAACTTGATTGAATTTCCCAAACCAAGCTCGTGGACTTTGCAAGGATTTTCGAAGACCACACACTTTACCTGTCTCCCCCTGAGCAACAAACCCAGGTGGTTGCTCCATAAATACTTCCTTTTGTAGATCACCAAGCATAAATGCATTCTTAATATCAAGTTGTAGGCGAAATTAGGTAAATGAAGTACAGACTGCAAAGACAAAGATGAGGTGAGGTCAACAGTGCCAGACCCAAGAACAGAGGATTTGGATTCGTCGGCCAAAGTAACATCCATCACAGACCCAGTGGATAAACTCGTTGAAAATAAATGGGGATAACCTATCATATGACTAGTAGCACCAGAGTCGATGACCCATTTGGTGGGACACGAAAGAAGACAAAAGTTCATGTTACTTGAGCCAACGCTAGCTATAGAAACAAAAAATCTGGAAGATGATCCTGTCATAGACAATAAAATGGAATCACTAGCCTTAGCAAATCAAGCGAACACAGCAATGAATCAGTTGAAAACAACTAATCAGTTCGAAAATTTCAGCAAATGAGACAAACGCCAAAACATAACAAAGCACAAGGAAAGGTTTGGAAGCAAGCACGAACTCGAAAACATCACTTAATCAATCAAATCTAGTTTGTACCACAACAAACTCAACAAGCAACTTACAGTTATGAAACAACCATATGCAACGCCAAAACACCAAAGAATGCAAAACTGTCACACCCCATAAAAAAGTGACCAAGTAGGCTTACAAAATCATCCACTCAACCAACGAATCAGCAAACTAGACCTAAACAGCAAACCAAAGTGAACAAATCCAATAAATTCATGAAATAAAACAACCACAATAACATCCCACACCAAATTCAAACATTCTTAATTGATGAAACCGTAACTCAAATTGTAAATCCCAAAGAAACAACACCACCACCTCAGAGAACAGTAGCAATAAACACCACCAACTAAGATTGAATTAAACAAAAAAAGTGGAACACCTTCTAGCCATAAAACCCGAGACAAAAATGTACTAGAAACTGAAATTCCAAGCACACAAACATGCTGGAAACATCAAGGAGAGAAAAACCCATAAAACGGGACAGAAAATGACCACCGCAAATGCCCTCACACGCCGGCGAGAGGCGGCTGCGGTCACACCTTGTAGCAGTGCGTGAGCCTCACGCGCCGCTCAAAATGGAACGAAACTTCCCAGGTGCGTAGATCAACGTCTAGGCTACCTGATGGTGGCGGTAGTGATGACTAATTCGCCAGAAAGTTTTTTTGGCGAAGGTTCGGCTACGGCGCAGTGGTGATATTTTCTAAACTTAGTGCTCTGATACCATGTTGTATTTTAATATGAGAAAAGAATCACATTACTTCATTCATTAGAGAAACTACATATATATAGGCTAAAGGAAACCCTAGCTAGGAATGGGAAAAGACTATAAAGGACAACACTTAAAACTAACTACTAAATAACCTCTAATAGAAGTCCCAGACAAATTTTCCCACATTGTAAATGTTTGTTGTTTTCAATTGAAAGTTATTTCCCCTAAATAAAGCAACAGTAAACATCGAACATAGCGATGCTATAGTGCTTCTACTATGAGTTTCACCATTATTGGACAAAGCACGACTTTTAGTAGAAGCTCAATTTGATTTGTTGTTTTGGAAGTTGATTTCTACCCTAGATATGTGCCCTTATAACATTGATCTTTTCTGTTGTTATATCTTGCTGCTTTCAGATATTCATATGCCCAAATCATGAATTTCTCTCAAGATTTTAGTTCCTATATTTACATTTTATGTAGTTGTCTTTTTTATGCTTAGTGTATTTTTAGTCCTTAGGTTTTTGTTCTCGACTTTAGATTGCATATCTTTACATCTTCCTTTCTTATATTCTTTTTTTTAGAGTTGTTTATGAAGCTATTACTTCTTTTCATTTTTTCAATGAAAAGTTTGTTTTTTTTGTTAAGAAAGAAATGTGCCATCTATTCGCTTCTTAATGCTGACAATATTGAGGAATCCATTAAAAAAATTCTTCGTTTAGATGAAGTTTCAAATGTTAATGTTGGTGATCTCGTAAATAGATCATCCCATTTTGCAGATTCAGATATTGAAGATAGCTTGAGTGTAGCAGTTTTGACTCTTCATGTAATCATCTTAAGGATGACATTTCCCTCCCCGTTGATGAAATCATATCCTGTTCAACTATGTTTGATCCACCCAAGTGTTCTCCCGTGCCTCAAATTCCTGGTCAACATTTTGTATTTCTAGTGAACGTCAATTTTCCCACATTGTAAATGTTTGTGGTTTTCAATGGAAGGTTATTACCCCCAAATAAAGCAGTAGTAAACATCAAACATAGGGATGCTATAGTGTTTCTACAATGAGTTTCACCATTATTGGACAAAGCACCGCTGCTAGTAGAAGCTCAATTTGATTTGTTGTTTTGGTAGCTGATTTCTACCCTAGATATATGCCTTATAACGTTGTTGATCTTTTATGTTGTTATATCTTGCTGCTTTCAGATATTCAGATGCTCAAATCATGAATCTCTCAAGATTTTAGTTCCTATATTTTAAATTTTATGCAGTTGTCTTTTTTATGCTTAGTGTATTTTAGTCCTTAGGCTTTGTTCTCGACTTTAGATTGCATATGTTTACATTTTCCTTTCTTCTATTCTTTTTTGAGAGTTTTTTATGAAGCTATTACTTCTTTTCACTTTTTCAATGAAAAGTTTGTTTTTTTTGTTTAAAAAGAAATGTGTCATCTGTTCTCTTCTTAATGCCGACAATATTGGGGAATCCATTAAAAAAATTCTCGCCTAGATGAAGTTTCAAATGTTGATGCTGGTGATCTCGTAAATATAATATATCAAGTGTTAATGGATGTAATTTAGGTCCATCTGGTACTTAAATTTTCAAGGCAATCATTCCTCATTTATCTGGTACTTAGAGTTCGAGGGTTCTATTCAGAAACATTATTATTCTCATATTGATGATTCTTCTCAATGAGAAGTTGTTGCTTCTTTCTTAAAAACAATAAATGATGATTCTCCTGATGTTGATTGTCCTATTGCTAATCTGGACTCTTTTTTTCAGCCGTGAGCCGTCTGATACTAATTCTCAAGCCCTTCACTCCATTTCTGAGAATATTCAATTGAATCTCAAATGCAACCCCTTCTGGTATTGAACAAATTTGACATTTATGACATTTCCTAGTGGCTATAAGAACATCAAGATCAAATACAATAGGCAAAGTATTTGGACTAGTTGAAGAAGTAGGAATACAAGAAGGATTATCAGTTGAGGAAGTAGGAGGGCAAGTAGGATTACTAAGTTTTTTTTAGTTTCACTCGTCGGAGTCTCAGATTGTAGTTGTGAAGGATCAACCATATGGCTTTGTTTCTTCTTTTCTTCCACGATAAAACTGGAACTCAAACTTGATCAATAGAATTATATCATAGTATTTCTCTCCCTGACTGAGAAGTTTCTAAACTTGGCATCGAAGGACTATTAACAACAGGACATAAAGACAAGGAAGAGGAGTAAAAGTATCCCAAAAATTCTCTTCCAAAATTGTCTTTTCCCCCAAAGAGAACTATGGGTACAATATGGTTGATTTTCAAGAAATGACACATCTATGCTGACAAAAGATTGGTTCGTCTGAGGATCAAGACATTTAACCTTTTTGACTTGATGAGTAACCCTAGAAAATACATTTTCAAATTCTAGTGAAATACTTGAGGAATATACTGGTGGCTTTTTTCCTTCTCCTCTTGTGGCATCGACAAGTAACCCTAGAAAATACATTTTCAAATTCTAGTGAAATACTTGAGGAATATACTGGTGGCTTGTTTCCTTCTCCTCTTGTGGCATCGACTTGCAAGCCTCCACCGACACCCACATCGCTTTTCTTCTCTATTGAGATTTATCTCTTTTTTTATTGATGTCAGAGCTTGAATTCATCGGTTTGTTTTTTTTTTTTTGCCCTCTGTTTTTTTACATATGTAATTGGCCCCTCCACATCCCCTTCACCTCCTATTCTTTCTTCTCGCATGTTTCGATCGGGTTTTATAACTCTTTCGCTTATAAATGAGTTTTTTTTTTCGTATAAAAAAATATAATAAATAAATAACATGTGGTAATTGAGTTGTGCTCATGTTTAATGAGTTAGGTGCTACTGCTACCCCAATGTTGGAACCTAAATTGCAGCTGGAAATTGGGTTGGATGTTTTTTCCGTGTGTGCACGGCCGCATTTATGACCTCTCTTGATTTGTGGCAGACTTTCATTTTACTTCTTGGAAGATCAATTTTTATGCATTGGTTGGTTGTCATATCCTAGACTCCTTGTCAATAAGCCTTTTTTTTTGTAGATTTTTTATGCTGATTAAATTTTGTTTTTTCTGTCAATCTCTTGTTAGAAGTTGGTGCTGTTTTAGTTTGAAAAGATCTTTCATCTTTAAGAAAGTAGAACTATAGCTGGAGATTGTTTTGCGGCAGTTAGTGGCTTTGGTTGTTTGACCTTTATTTTGCTAAGATTGAGAATTGTGTAATGGGATGCTTTTTGTCATTGTCTTTTAGCATTAGTCACTTTTCATTTCTGGTTAAAGAAGTATTTATTTTGGATTTTGGTTAGAAACTTACCATTTGTATTCTTCCATTGACCGAACTTTCAAGTTAAATAAATAGGAATGATGCTCGAGAATCCAGTTAAGTAGTTCTGTACTTATATGTTTATATTATTTACGTGTAGGTTTGGAAGAACGGTTGCTTGTTTCTATTATTCTGCAATTAATGTTCAGTCTGTTCTTATCCCACCACCCATACTTGAATTTCACCATAATCTAGAGTGGATTAATAAGGAAGCGAATGAGGTGCGGCACCTTTGAATTCAACTGCAAATGTAAGCTTAAAGCCACTGATTATATTTTGGAAGAAAGGTTAATCAGTATTTGGTCTCTTCTATTAGGTACATAACAGGGCTAAAACTCTGTTTGCTGAAGTCTATAAAGCCCTTCCTCTGATTTCAAATAGTGAATCTGGCACAGAGCAAGAGGATTTTGGGGTCAATGAATTGAGCCATTATGTTCAAGAACTTGAGCAAATCATTAATATGGAGAAAGAAGATTTTGAGGTAGGTTTTCAGATTGTGGTTTTATTTCAAATTTTATCTTAATTTTGAAACTGACGATAACACACTATGCTTGTTTTGTGAGGGCATATTGTTATCTGCTGAAGTTTCATATGCATTCATGTGCATTTAATTGCTTGATGCACCGATGGAAATCATGTATGAACTTGATTCACTAATCCTATCAGCTAATTTGAACATTTTATTTGAAATATTGAATTAAATAGTTTCTTTTGACATCATTTTTAACGTTGACTTTATGCGAAACGATGGTTGCTGTCTTAATTTGTAGGTAAACACACAATTTTCCTATTATGTAATATAGATTATTATAGCCCTTTGATGTTTCTAGGTTGCTAGTTTCCCTGTGCATTCTAAAGCTTTCTTTGTGGAGAATGAATCTTTATGCTTTGGTTCTTTTGATTTATACCATCAAGTCATTATAGGTTCATTTTAATGAAGTACTATCTGGAGAGATTCCAATGAAGAATGAGGTACCATCGGGAGAGGTAGCAATTGGCCAGCCTAGAGTAGATATTTTTGAGATCAATAAACTGCATATGCACATTCTCTTTCTTTCATATACTTGGGTTCAGCGTTTAAGTTATGCAGCCGCTTTGAGAAAGAAGAATTCCCCAGATAGTTCGAGCAATGACATCCCAATGCTTGATGAAAGGGAACTTGATTCACTGGAACAGTTGGTGAAATTTACTCTTGAATCAAGGCGGGATGAATTCTTTGATGGTTTTGATAATGATTCTCTACCTTTAGAGTCAAAACACGAGATTGATCTTGGCAATGGAGGTTATTCAGGGCAAATTAGCCAGTCGAATGAAATGGAGAGGGAAAAGAATTTTGACCTTGGTGGACTGGAAGATGACCTTTGTCTTTCTCCTCCAGTTAACTCATTGCCTCGAGATGTTGAAATCTCCATACGAAGGGCAAATTCAGAAGGAGAATATCCAATTATGGCTGATTTATCTGATACCCTTGATGCAGTTTGGAAAGGTGTAATTCAACCAAATAATGGAACATTTGAGGAGAGTAGTAATTCTGGAACTAACTCGGCCGTTCTGGAGCCGTTGTCAGGACAGTCTGATATGGAGAAGTGTAATGCAACCTTAGTTGGTGTTGAGACGGCTCATCTTCTTCATTCTGCCATAGTTTTAACAAGAGATGATTCTGTTGAGACAATTACGAGTTCTACAGCAATGCCTTCATCAGCATATTATTCAAAAATTTCATTGTTGAATATTCCAAAGCCCAACATTTCTGACTATGATCATGCCTTCATTTCATCGTTCAGGGAATTGGAAAAGCAAGAGCACCTTAGGCTACTTATGCATGTTGGTTCCAGTGACACTGTTGTTCCTGTCTATGACGAGGAGCCCACTAGTATTATTGCATATTCATTGCTTATGCCAGAGTATAATGTTCAGATGTCTGAGCCAGAGAAAGCACTAGAACCTGGGGATACTTCACGGTCCTTGCCTATTATTGGTTCTGCTAAATTGAGCTCACTTAAAACATCTGACGAAGAAGGTTCAGGTACCATTTTATCCATGCCTAAAACGGAACGTCAGTTTGAGGATCGAGTGTCGATTGCTCAGAATAAGCATACAAGAGTTAGTTTTGTAAATGAGACTCCCTTGGGGCCGGTGAAATACACCATAACTGGCTTCTTTGCTAAGCGGTTTGAGGCATTAAGAAAAAGGTGTTGCCCTTCGGAGTTAGATTATGTAAGGTCTCTTAGTCGTTGTAAGAGGTGGGGAGCCCAAGGTGGCAAAAGCAATGTTTTCTTTGCAAAAACTTTAGATGAGCGATTCATTATCAAACAGGTGACAAAAATAGAGTTGCAATCATTCATTCAGTTTGCATCAGCTTATTTTATGTATTTGTCTGAAGCAACTCGCACAACTTGTCCAACTTGCTTGGCAAAGGTCGTGGGGATATATCAGGTACTATAATTTTTAATTTTCTTTCATCTTAGCCGTTATTGACTTTCTCGTTGAAATCAAGGAAGTTAAAAAAAAATGATTTTAAATGACATGAGTTGAAAAATAGAAATGATAACGATATGGCTTTTGTTTTTCTTTCTTAGTCATAATTTGTTTGACGAGAAGATTGATTTTTCATTAACTTTGTAATCCTCTAACCATCGTAATCAAGAAAGTCTTTTTCAAGTAAGAATAATTTGATTCGAAACCTTTTTCATAATTATATTCTTTGGGAAATAAATCTTGAAACTTTGTGTGTCATTAAATTATCTTCTTGGGTTTGTTTTCGTTGTCTTGAAGGTGTCCTCAAAATATCTAAAGGGAGGAAAAGAATGCAAGATTGACATTTTAGTTATGGAGAATCTTCTGTTTCGGCATAATGTCACACAACTTTATGATCTAAAAGGATCTTCTCGATCACGCTATAATTCAGATACAAGCGGAAAGAATACAGTTCTTTTGGATCAGAATCTGATTGAATCTATGCCAACCTCTCCAATATTTTTGGGCAGCAAGGCAAAGCGGTTGTTAGAAAGAGCTGTTTGGAATGATACATCATTTTTAGCTGTAAGTTTAATTTATCTTTTTTGTATAGTGAATCATGCTACTACTAAACATTGTATAGTGAATGAACATCTCTATTTCAAGTCTAGAATGATTTAGTTGAATACTTGCTATTTTTATATATATATATACTCAATACTGGTACTAGAGTAGACTAATAGGAACGCTAGTAGGGTAGAATAAGGAATATGATCACAAGGTATCTTAATTCTTCTATTTCTTTTTCCATCTTATTATAACTACGTAGCTTGTGAACATACTATTAAGTGTTTTGGTTTATAGATTAAGTTTCTACAATTCTGGATTAAGGAATGACTATGTTCACACATGATTCTACATATATTTAGGGTACGAATTGGCTAATTATGTGGGTATAATATTTCTGAGTGATTTAATTTATATACATACATACATGCATACATGAAATCAAACACATATTCGTTTCTTCATATAATTCAAGCATAGATTACATAAGTTCAAGTTTGAGAGGCTTCACAAACTTTGACTGTTTAAAATCCCGAGCTGAAAACCCGTTTGTTTATCATCCATTTAGCTGGCTGATGTATTCATATGAGATTATATGCCCAACTTACATATTGTCTCAAATTGTTGCTGCAGTCAATTCATGTCATGGACTACTCACTATTGGTCGGGCTAGATGAGGAAAAGCAAGAGCTGGTTGTGGGGATTATTGATTATATGAGGCAGTATACTTGGGATAAGCACCTTGAAACTTGGTTGAAGAACTCGGGCATTCTAGGTGGGCCCAAGAATGCTGCTCCTACTGTCATCTCACCTCATCAATACAAGAAACGTTTCAGAAAAGCTATGACTACATATTTTCTTATGGTTCCAGATAGTTGGCCCTCCTCAACAGTCAGCCCTAGTGAGTCCCACCCGGATCTCTGTGACGATGGTTCCGAACCTGAGAATTCGTGGGATGGGTAAGTTTGCAAATATTTTCAATATAGTGTGCATAATTTCTCTTCATTGTTTGTTATTTTTCAGTCTTTGGACAACTTAGTTTACTCAATGATTTGAACCACTAATGATTCATTTTAAAACGGAAAAGGGCTTTTCTTTCTCAACGATGTTACTTTTTTTCTTTTTTTTTTGAAACTGGGAAATCGGAGCTTCGCTCCATTATACCCAGGAGTAACCACCCTCATCCCTAGACCAGGCATCGGGAGACATCGAAGGAATGGAAACACGGAAGGTATCTAGAAATAAATGTCACAGGTGAGACTCAAATCTGGAATCTTGAGGAGAGCACCCACTCAAAGCTCAAGCCTTCGACCATCGTGTTCAATGATGTTACTTTCTAATACGCTGATAAGGCTGGGCCTAGGGCTCTATGTTAGCAATTACTTGGAGAGTAAGTTAGGATTTTGGTTATAAATAGAGTGGGTTAGGGTAGGGTTTAGGCATCCAAATTAATGAGTTATTGAGTGATTTATTGTACGGCTCAAGAGATTCTCTTGAGAGGGGAGGTTCCAAGACCTATTAAACTTGGCTTATCTTTATTCTTTTACTTTTGATATAATCTAGATCCTAGATCATATCATACGCTTAGACACTACTATATTTTGGGAGAGTTTGGCATAACTAAAAGCAGTTTTGCTATAGCCAAAATCACTTTTTACACAAAATTATCATTTTTGGGAGTAACTTGAACAATTAAAATATTTTGTGAGAAGTCCTTTTAGTGTTTATACCTATATGTTTTTTGTCACTTCTTTTTACATTGTAAATTGGTTGATCAATTTTTTTTTTATCAAGATTTAATTACTAGATGACAGAAACTTGAAATTATCAACTCAGCAAGACTTTTTAACAAAATTTTTGTTTGTTTATCTGCAGCAAACATGATAGTGAACAGAGTGATTGGTGA

mRNA sequence

GACAGAAAGTACACAACAGAATGGAGAGAGAAACTAGAGAGAGAAATTAGAGTCGCCATCGCCATCGCCATTACCAGATTTCCAGAACGTGATCGAACGATCATCAACGGAGTTCAGGCAAGACGACGGCGGCGAAGAGCGCGAAACACCGCCTTGATTCCATTTCTACAAGTTCTTGAGGTACGTCTAACAAATTCCGAACCAGCACCGTCAAATCATCCAAGCCGTGTGCCTCTTTCGTCCAGACTCATCGATTGAGATTTGGGCGGAATGAATGTTTCGTTGCGTCATGTTCGCTGGGTTCACTCGCCTGGAAAAGAAGAGCGTGTTTGATTTTGGTTGATTAATTTAAAAATGTCTAAATCAGTTCTTATACGTGAGAGGTGGAAGTGATTGTTTCGTATTGCAAATGTGGAATTTCGTAACCAGTGATTTCATATTAGATCGAGAAGGTTATTTTTTTATATATAAAAGAAAATAGAGAAGGTAATCAGGTGGTTGATGAGCAGATTGGGATGAGTGGAGTATCCACATCATCATCAACCTCATCAAATGGGCCATCCTGAAAACAAATTATCCGAACTTGTGGGCTTGGCAAAGTCATGGATATCATCATGGAGGTCTAACTCGGAAAATGTCGCGAGGGACTTTTGGATGCCTGACAGAAGTTGTAGAGTATGCTATGAATGTGACTCTCAATTCACATTTATCAAACGGCGGCATCATTGCCGTTTCTGTGGACGAGTTTTCTGTTCTAAATGTACTTCAAACTTCATTCGTGCTCCATCCACCGATTCAACGAATTGTATGGAGGATTCTGAGAAGATCAGAGTTTGTAATTATTGTTTTAAGCAGTTGGGGAGGGAGCCATTTGCCTTGCAAGATGGGGACAAAGCTACCAGCTCTGCTATTAGTCTATCTTCTTCATCGTCCAGCATGGATAGCAGTAAGTCCGATTACACCTGTCAAAGTGCACTCAGCAATATTAATTCAACGCCTTGCTCAAATAGTCATCAATATTATGACCCGTATTGTTCGTTTCCTATCACTGATGAGCAAGATAGTTTAAGAAGTGGGAAGAGCATCAATCGGACTACACCTCGCACGGCTTCAACATCTGGTTATTATTATGATGGTTATTACAGGGGTGATGATGAGGATGATGATTATTATTATGGAACGTATCGCTCAGATTCAGAAACCAAGCATATATCCCAGTCTGATGATTATTATTGTGCGGTAAACGCCGACGAGGGCTTAAATCTTGATGGAGAAAACATCTGTACTAAGGGTTCAGCAGTGATTTTTGACACTCCGTGTCCTAGGAGTAATGAGGAATTATCTAGTGAAGACTCTGATGAAAATGATAAAGGCTCTGAATGTGACTTTCCTGCTTATGGCATGCAAAATAAGAATAATGAACCTATGGATTTTCAGAACAATGGGGTTCTCTGGCTTCCTCCAGAGCCAGAAGATGAGGAAGATGATAAACTTCTTTTTTTTGATGATGATAATGATTCCCGTTCTCCAGGTAAATGGGCACCTTCTTTTTCCTTGGGCAGTGGGGAATACCCCATGAAGGGAAGGTCAAGTGAGGAGTACAGGAAAACTGCAAAATACGTCGTAGAAGGGCATTTTAGAGCTCTCGTTGGTCAACTTTTGGAGGCTGAGGATCTTCCTATAGGTGATGGTCCCAGTGGAGATGGCTGGTTAGATATTATAACATATCTGTCCTGGGAAGCAGCTACAGTTCTGAAGCCAGATATGAGCAAGAGTGGACCTATGGATCCTAGTGGATATGTTAAGGTTAAATGCGTTGCTGGTGGAAAACGTAGTGAGAGCATTATTGTTAAAGGCATCATTTGCAAGAAGAATGTTGCACACAAGCGGATGACGAGGGAAATATCTAAATCACGCATTTTTGTCCTTGGGGGAGCTTTGGAGTATCAACGTGTTATTAATCACGTGTCAAGTCTTGATACTTTGATGCAGCAGGAACTTGACCATTTGAAGATGGCTGTTGCTAAGATTGATGCCCATCATCCTAATATTCTTTTGGTAGAGAGATCAGTGTCTCGCCATGCTCAGGAGTACCTTGTCGCAAAAAACATCACTGTTGTTCTAAATATTAAACGATCACTCTTAGAGCGTATTGCTTCCTGCACTGGGGCAAGTATAATTCCTACTGTTGATCATGTTGCCTCGCTGAAACTTGGTTATTGTGATGCATTTCGTGTGGAGACATTTGAAGTACATGACACTGGTGATGGCCAAGCTAGCAAGACATCAGCGAAGACTTTGATGTTCTTTGAGGGATGTCCAAAGCCTCTATGTTGTACTGTTTTGCTAAGGGGTGCCAGTAAAGATGAGCTTAAAAAAGTTAAACATGTTGTTCAATATGCAACTTTTGCTGCCTATCACCTGGCTCTAGAAACATGCTTTCTGGCTGATGAAGGCGCTTCAGTTCCAGAGCTCCCATCGAAGTCCCCATTAACAGTTATTCTTCCTCATAATCCATCAAATACAGACAGGTCCATCTCCTCTATTCCTGGCTTTACTTGCCCATCACCTGAACTTAACACGGAAATCGGGCAATCTAGAAAAGATGCTGTTCCAAACAAGGACTCATCTATAATTAGGAATAATGTTGCCAATTTGGACTCTTTAGAGCCTGAATTTATTGAGGCAAATAGAAATCTGGAACGCCAGGAATATTCAATTGGCACGATGACTATCAAGGAAGCAGTAGGGGCAGATGATGATACAATTTCTAGTTCTGGTAAAGATGAAGCTTCGAGTCAACGTTGTAACCCTATCTCTATAAGCATCGATTCGTTGGATGGAAGTACACAAGCTTCTAATGATTCAACATCTCAAAGACCTGAAAGCCCGAAGGCTAATCCAGTGACCACCAAATCTTCAAGGGAAGATTTCCCTTTGTCACCTTCTGACCAACAAAACATTTTGGTTTCTTTGTCTACGCGTTGTGTGTGGAAGGGAACTACTTGTGATCGTGCTCATCTCTTACGAATTAAATACTATGGGAGCTTTGACGAACCCTTGGGTCGGTTTCTACGAGATAATTTGTTTGATCAGAGTTATCGATGTCATTCATGTAGTATGCCCTCCGAGGCACATGTGCATTGTTATACGCATCCTCAAGGCAGTCTTACAATATCTGCTAAGAAACTCCTGACTTTGTTCTACCGGGAACCTGAAGGTAAAATTTGGATGTGGCATCGGTGTTTGAAGTGCCCTCGGACCAATGGCTTCCCACCAGCCACTAGGAGAATTATTATCTTTTCAAATAATGCAGCTGCCAGCCGTGTTGCAGGTTGTGGCCATTTCCTTCATAGAGACTGTCTCCGTTTCTATGGGTTTGGAAGAACGGTTGCTTGTTTCTATTATTCTGCAATTAATGTTCAGTCTGTTCTTATCCCACCACCCATACTTGAATTTCACCATAATCTAGAGTGGATTAATAAGGAAGCGAATGAGGTACATAACAGGGCTAAAACTCTGTTTGCTGAAGTCTATAAAGCCCTTCCTCTGATTTCAAATAGTGAATCTGGCACAGAGCAAGAGGATTTTGGGGTCAATGAATTGAGCCATTATGTTCAAGAACTTGAGCAAATCATTAATATGGAGAAAGAAGATTTTGAGGTTCATTTTAATGAAGTACTATCTGGAGAGATTCCAATGAAGAATGAGGTACCATCGGGAGAGGTAGCAATTGGCCAGCCTAGAGTAGATATTTTTGAGATCAATAAACTGCATATGCACATTCTCTTTCTTTCATATACTTGGGTTCAGCGTTTAAGTTATGCAGCCGCTTTGAGAAAGAAGAATTCCCCAGATAGTTCGAGCAATGACATCCCAATGCTTGATGAAAGGGAACTTGATTCACTGGAACAGTTGGTGAAATTTACTCTTGAATCAAGGCGGGATGAATTCTTTGATGGTTTTGATAATGATTCTCTACCTTTAGAGTCAAAACACGAGATTGATCTTGGCAATGGAGGTTATTCAGGGCAAATTAGCCAGTCGAATGAAATGGAGAGGGAAAAGAATTTTGACCTTGGTGGACTGGAAGATGACCTTTGTCTTTCTCCTCCAGTTAACTCATTGCCTCGAGATGTTGAAATCTCCATACGAAGGGCAAATTCAGAAGGAGAATATCCAATTATGGCTGATTTATCTGATACCCTTGATGCAGTTTGGAAAGGTGTAATTCAACCAAATAATGGAACATTTGAGGAGAGTAGTAATTCTGGAACTAACTCGGCCGTTCTGGAGCCGTTGTCAGGACAGTCTGATATGGAGAAGTGTAATGCAACCTTAGTTGGTGTTGAGACGGCTCATCTTCTTCATTCTGCCATAGTTTTAACAAGAGATGATTCTGTTGAGACAATTACGAGTTCTACAGCAATGCCTTCATCAGCATATTATTCAAAAATTTCATTGTTGAATATTCCAAAGCCCAACATTTCTGACTATGATCATGCCTTCATTTCATCGTTCAGGGAATTGGAAAAGCAAGAGCACCTTAGGCTACTTATGCATGTTGGTTCCAGTGACACTGTTGTTCCTGTCTATGACGAGGAGCCCACTAGTATTATTGCATATTCATTGCTTATGCCAGAGTATAATGTTCAGATGTCTGAGCCAGAGAAAGCACTAGAACCTGGGGATACTTCACGGTCCTTGCCTATTATTGGTTCTGCTAAATTGAGCTCACTTAAAACATCTGACGAAGAAGGTTCAGGTACCATTTTATCCATGCCTAAAACGGAACGTCAGTTTGAGGATCGAGTGTCGATTGCTCAGAATAAGCATACAAGAGTTAGTTTTGTAAATGAGACTCCCTTGGGGCCGGTGAAATACACCATAACTGGCTTCTTTGCTAAGCGGTTTGAGGCATTAAGAAAAAGGTGTTGCCCTTCGGAGTTAGATTATGTAAGGTCTCTTAGTCGTTGTAAGAGGTGGGGAGCCCAAGGTGGCAAAAGCAATGTTTTCTTTGCAAAAACTTTAGATGAGCGATTCATTATCAAACAGGTGACAAAAATAGAGTTGCAATCATTCATTCAGTTTGCATCAGCTTATTTTATGTATTTGTCTGAAGCAACTCGCACAACTTGTCCAACTTGCTTGGCAAAGGTCGTGGGGATATATCAGGTGTCCTCAAAATATCTAAAGGGAGGAAAAGAATGCAAGATTGACATTTTAGTTATGGAGAATCTTCTGTTTCGGCATAATGTCACACAACTTTATGATCTAAAAGGATCTTCTCGATCACGCTATAATTCAGATACAAGCGGAAAGAATACAGTTCTTTTGGATCAGAATCTGATTGAATCTATGCCAACCTCTCCAATATTTTTGGGCAGCAAGGCAAAGCGGTTGTTAGAAAGAGCTGTTTGGAATGATACATCATTTTTAGCTTCAATTCATGTCATGGACTACTCACTATTGGTCGGGCTAGATGAGGAAAAGCAAGAGCTGGTTGTGGGGATTATTGATTATATGAGGCAGTATACTTGGGATAAGCACCTTGAAACTTGGTTGAAGAACTCGGGCATTCTAGGTGGGCCCAAGAATGCTGCTCCTACTGTCATCTCACCTCATCAATACAAGAAACGTTTCAGAAAAGCTATGACTACATATTTTCTTATGGTTCCAGATAGTTGGCCCTCCTCAACAGTCAGCCCTAGTGAGTCCCACCCGGATCTCTGTGACGATGGTTCCGAACCTGAGAATTCGTGGGATGGCAAACATGATAGTGAACAGAGTGATTGGTGA

Coding sequence (CDS)

ATGGGCCATCCTGAAAACAAATTATCCGAACTTGTGGGCTTGGCAAAGTCATGGATATCATCATGGAGGTCTAACTCGGAAAATGTCGCGAGGGACTTTTGGATGCCTGACAGAAGTTGTAGAGTATGCTATGAATGTGACTCTCAATTCACATTTATCAAACGGCGGCATCATTGCCGTTTCTGTGGACGAGTTTTCTGTTCTAAATGTACTTCAAACTTCATTCGTGCTCCATCCACCGATTCAACGAATTGTATGGAGGATTCTGAGAAGATCAGAGTTTGTAATTATTGTTTTAAGCAGTTGGGGAGGGAGCCATTTGCCTTGCAAGATGGGGACAAAGCTACCAGCTCTGCTATTAGTCTATCTTCTTCATCGTCCAGCATGGATAGCAGTAAGTCCGATTACACCTGTCAAAGTGCACTCAGCAATATTAATTCAACGCCTTGCTCAAATAGTCATCAATATTATGACCCGTATTGTTCGTTTCCTATCACTGATGAGCAAGATAGTTTAAGAAGTGGGAAGAGCATCAATCGGACTACACCTCGCACGGCTTCAACATCTGGTTATTATTATGATGGTTATTACAGGGGTGATGATGAGGATGATGATTATTATTATGGAACGTATCGCTCAGATTCAGAAACCAAGCATATATCCCAGTCTGATGATTATTATTGTGCGGTAAACGCCGACGAGGGCTTAAATCTTGATGGAGAAAACATCTGTACTAAGGGTTCAGCAGTGATTTTTGACACTCCGTGTCCTAGGAGTAATGAGGAATTATCTAGTGAAGACTCTGATGAAAATGATAAAGGCTCTGAATGTGACTTTCCTGCTTATGGCATGCAAAATAAGAATAATGAACCTATGGATTTTCAGAACAATGGGGTTCTCTGGCTTCCTCCAGAGCCAGAAGATGAGGAAGATGATAAACTTCTTTTTTTTGATGATGATAATGATTCCCGTTCTCCAGGTAAATGGGCACCTTCTTTTTCCTTGGGCAGTGGGGAATACCCCATGAAGGGAAGGTCAAGTGAGGAGTACAGGAAAACTGCAAAATACGTCGTAGAAGGGCATTTTAGAGCTCTCGTTGGTCAACTTTTGGAGGCTGAGGATCTTCCTATAGGTGATGGTCCCAGTGGAGATGGCTGGTTAGATATTATAACATATCTGTCCTGGGAAGCAGCTACAGTTCTGAAGCCAGATATGAGCAAGAGTGGACCTATGGATCCTAGTGGATATGTTAAGGTTAAATGCGTTGCTGGTGGAAAACGTAGTGAGAGCATTATTGTTAAAGGCATCATTTGCAAGAAGAATGTTGCACACAAGCGGATGACGAGGGAAATATCTAAATCACGCATTTTTGTCCTTGGGGGAGCTTTGGAGTATCAACGTGTTATTAATCACGTGTCAAGTCTTGATACTTTGATGCAGCAGGAACTTGACCATTTGAAGATGGCTGTTGCTAAGATTGATGCCCATCATCCTAATATTCTTTTGGTAGAGAGATCAGTGTCTCGCCATGCTCAGGAGTACCTTGTCGCAAAAAACATCACTGTTGTTCTAAATATTAAACGATCACTCTTAGAGCGTATTGCTTCCTGCACTGGGGCAAGTATAATTCCTACTGTTGATCATGTTGCCTCGCTGAAACTTGGTTATTGTGATGCATTTCGTGTGGAGACATTTGAAGTACATGACACTGGTGATGGCCAAGCTAGCAAGACATCAGCGAAGACTTTGATGTTCTTTGAGGGATGTCCAAAGCCTCTATGTTGTACTGTTTTGCTAAGGGGTGCCAGTAAAGATGAGCTTAAAAAAGTTAAACATGTTGTTCAATATGCAACTTTTGCTGCCTATCACCTGGCTCTAGAAACATGCTTTCTGGCTGATGAAGGCGCTTCAGTTCCAGAGCTCCCATCGAAGTCCCCATTAACAGTTATTCTTCCTCATAATCCATCAAATACAGACAGGTCCATCTCCTCTATTCCTGGCTTTACTTGCCCATCACCTGAACTTAACACGGAAATCGGGCAATCTAGAAAAGATGCTGTTCCAAACAAGGACTCATCTATAATTAGGAATAATGTTGCCAATTTGGACTCTTTAGAGCCTGAATTTATTGAGGCAAATAGAAATCTGGAACGCCAGGAATATTCAATTGGCACGATGACTATCAAGGAAGCAGTAGGGGCAGATGATGATACAATTTCTAGTTCTGGTAAAGATGAAGCTTCGAGTCAACGTTGTAACCCTATCTCTATAAGCATCGATTCGTTGGATGGAAGTACACAAGCTTCTAATGATTCAACATCTCAAAGACCTGAAAGCCCGAAGGCTAATCCAGTGACCACCAAATCTTCAAGGGAAGATTTCCCTTTGTCACCTTCTGACCAACAAAACATTTTGGTTTCTTTGTCTACGCGTTGTGTGTGGAAGGGAACTACTTGTGATCGTGCTCATCTCTTACGAATTAAATACTATGGGAGCTTTGACGAACCCTTGGGTCGGTTTCTACGAGATAATTTGTTTGATCAGAGTTATCGATGTCATTCATGTAGTATGCCCTCCGAGGCACATGTGCATTGTTATACGCATCCTCAAGGCAGTCTTACAATATCTGCTAAGAAACTCCTGACTTTGTTCTACCGGGAACCTGAAGGTAAAATTTGGATGTGGCATCGGTGTTTGAAGTGCCCTCGGACCAATGGCTTCCCACCAGCCACTAGGAGAATTATTATCTTTTCAAATAATGCAGCTGCCAGCCGTGTTGCAGGTTGTGGCCATTTCCTTCATAGAGACTGTCTCCGTTTCTATGGGTTTGGAAGAACGGTTGCTTGTTTCTATTATTCTGCAATTAATGTTCAGTCTGTTCTTATCCCACCACCCATACTTGAATTTCACCATAATCTAGAGTGGATTAATAAGGAAGCGAATGAGGTACATAACAGGGCTAAAACTCTGTTTGCTGAAGTCTATAAAGCCCTTCCTCTGATTTCAAATAGTGAATCTGGCACAGAGCAAGAGGATTTTGGGGTCAATGAATTGAGCCATTATGTTCAAGAACTTGAGCAAATCATTAATATGGAGAAAGAAGATTTTGAGGTTCATTTTAATGAAGTACTATCTGGAGAGATTCCAATGAAGAATGAGGTACCATCGGGAGAGGTAGCAATTGGCCAGCCTAGAGTAGATATTTTTGAGATCAATAAACTGCATATGCACATTCTCTTTCTTTCATATACTTGGGTTCAGCGTTTAAGTTATGCAGCCGCTTTGAGAAAGAAGAATTCCCCAGATAGTTCGAGCAATGACATCCCAATGCTTGATGAAAGGGAACTTGATTCACTGGAACAGTTGGTGAAATTTACTCTTGAATCAAGGCGGGATGAATTCTTTGATGGTTTTGATAATGATTCTCTACCTTTAGAGTCAAAACACGAGATTGATCTTGGCAATGGAGGTTATTCAGGGCAAATTAGCCAGTCGAATGAAATGGAGAGGGAAAAGAATTTTGACCTTGGTGGACTGGAAGATGACCTTTGTCTTTCTCCTCCAGTTAACTCATTGCCTCGAGATGTTGAAATCTCCATACGAAGGGCAAATTCAGAAGGAGAATATCCAATTATGGCTGATTTATCTGATACCCTTGATGCAGTTTGGAAAGGTGTAATTCAACCAAATAATGGAACATTTGAGGAGAGTAGTAATTCTGGAACTAACTCGGCCGTTCTGGAGCCGTTGTCAGGACAGTCTGATATGGAGAAGTGTAATGCAACCTTAGTTGGTGTTGAGACGGCTCATCTTCTTCATTCTGCCATAGTTTTAACAAGAGATGATTCTGTTGAGACAATTACGAGTTCTACAGCAATGCCTTCATCAGCATATTATTCAAAAATTTCATTGTTGAATATTCCAAAGCCCAACATTTCTGACTATGATCATGCCTTCATTTCATCGTTCAGGGAATTGGAAAAGCAAGAGCACCTTAGGCTACTTATGCATGTTGGTTCCAGTGACACTGTTGTTCCTGTCTATGACGAGGAGCCCACTAGTATTATTGCATATTCATTGCTTATGCCAGAGTATAATGTTCAGATGTCTGAGCCAGAGAAAGCACTAGAACCTGGGGATACTTCACGGTCCTTGCCTATTATTGGTTCTGCTAAATTGAGCTCACTTAAAACATCTGACGAAGAAGGTTCAGGTACCATTTTATCCATGCCTAAAACGGAACGTCAGTTTGAGGATCGAGTGTCGATTGCTCAGAATAAGCATACAAGAGTTAGTTTTGTAAATGAGACTCCCTTGGGGCCGGTGAAATACACCATAACTGGCTTCTTTGCTAAGCGGTTTGAGGCATTAAGAAAAAGGTGTTGCCCTTCGGAGTTAGATTATGTAAGGTCTCTTAGTCGTTGTAAGAGGTGGGGAGCCCAAGGTGGCAAAAGCAATGTTTTCTTTGCAAAAACTTTAGATGAGCGATTCATTATCAAACAGGTGACAAAAATAGAGTTGCAATCATTCATTCAGTTTGCATCAGCTTATTTTATGTATTTGTCTGAAGCAACTCGCACAACTTGTCCAACTTGCTTGGCAAAGGTCGTGGGGATATATCAGGTGTCCTCAAAATATCTAAAGGGAGGAAAAGAATGCAAGATTGACATTTTAGTTATGGAGAATCTTCTGTTTCGGCATAATGTCACACAACTTTATGATCTAAAAGGATCTTCTCGATCACGCTATAATTCAGATACAAGCGGAAAGAATACAGTTCTTTTGGATCAGAATCTGATTGAATCTATGCCAACCTCTCCAATATTTTTGGGCAGCAAGGCAAAGCGGTTGTTAGAAAGAGCTGTTTGGAATGATACATCATTTTTAGCTTCAATTCATGTCATGGACTACTCACTATTGGTCGGGCTAGATGAGGAAAAGCAAGAGCTGGTTGTGGGGATTATTGATTATATGAGGCAGTATACTTGGGATAAGCACCTTGAAACTTGGTTGAAGAACTCGGGCATTCTAGGTGGGCCCAAGAATGCTGCTCCTACTGTCATCTCACCTCATCAATACAAGAAACGTTTCAGAAAAGCTATGACTACATATTTTCTTATGGTTCCAGATAGTTGGCCCTCCTCAACAGTCAGCCCTAGTGAGTCCCACCCGGATCTCTGTGACGATGGTTCCGAACCTGAGAATTCGTGGGATGGCAAACATGATAGTGAACAGAGTGATTGGTGA

Protein sequence

MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAISLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINRTTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDGENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDKLLFFDDDNDSRSPGKWAPSFSLGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNTDRSISSIPGFTCPSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIKEAVGADDDTISSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIIIFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHHNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSDW
Homology
BLAST of Sed0006163 vs. NCBI nr
Match: XP_038877586.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Benincasa hispida])

HSP 1 Score: 2745.3 bits (7115), Expect = 0.0e+00
Identity = 1405/1817 (77.33%), Postives = 1536/1817 (84.53%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENK+SELVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKISELVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCTSNFI APSTD +N  E+SEKIRVCNYCFKQ G+EP  LQDGDKATSSAI
Sbjct: 61   RCGRVFCSKCTSNFIPAPSTDPSNAREESEKIRVCNYCFKQWGKEPSDLQDGDKATSSAI 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            S SSSS+SM S+KS YTC SA+SN  STPCSN HQYYDPYC   ITDEQD+LR+GK+INR
Sbjct: 121  SRSSSSTSMGSTKSGYTCHSAISNSASTPCSNGHQYYDPYCPGAITDEQDNLRTGKTINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S +Y  GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN DEGLNL+ 
Sbjct: 181  TTSLMTSSSSFY--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLER 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            EN  TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK+NEPMDFQNNG+L
Sbjct: 241  ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYVMQNKDNEPMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKWA-PSFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
            WLPPEPEDEED+K+L F+DD+D+ +PG+W     SL SGE  MKG+SSE+Y+KTAK VVE
Sbjct: 301  WLPPEPEDEEDEKVLLFEDDDDACAPGEWGYLHSSLSSGECHMKGKSSEDYKKTAKNVVE 360

Query: 361  GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
            GHFRALV QLLEAE+LP+GD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361  GHFRALVSQLLEAENLPVGDEPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420

Query: 421  KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
            KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421  KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480

Query: 481  QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
            QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIASCTG
Sbjct: 481  QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTG 540

Query: 541  ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
            A+IIPTVDHVASLKLGYCDAFRVETF E HD GDGQ  K S KTLMFFEGCPKPLCCT+L
Sbjct: 541  ANIIPTVDHVASLKLGYCDAFRVETFVEEHDNGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600

Query: 601  LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
            LRGAS +ELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP KSP+TV LPHNPSN 
Sbjct: 601  LRGASGEELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNI 660

Query: 661  DRSISSIPGFTCPS------PELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEPEF--- 720
            DRSIS+IPGFTCPS      PE   EIGQS KDA+ ++DSSII +NV NL+SLEP     
Sbjct: 661  DRSISAIPGFTCPSPVKSPEPEPIMEIGQSGKDAILSRDSSIISHNVVNLNSLEPTLRLL 720

Query: 721  ------------------------------IEANRNLERQEYSIGTMTIKEAVGADDDTI 780
                                           E  RNL+R+EYS+G MTIKE  G DDDT+
Sbjct: 721  KSNATSSSMSLLKQDVSTADCNYMLSSKFSSEERRNLDRKEYSMGMMTIKEEAGDDDDTV 780

Query: 781  SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
             SSGK E  S+  N I IS + LD + Q+SNDSTSQ PES  AN V+TKSSREDFPLSPS
Sbjct: 781  RSSGKGEPLSRFANSIQISRNLLDANAQSSNDSTSQGPESSNANLVSTKSSREDFPLSPS 840

Query: 841  DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
            DQQNILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLG+FLRDNLFDQ+YRCHSC MPS
Sbjct: 841  DQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGQFLRDNLFDQNYRCHSCGMPS 900

Query: 901  EAHVHCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
            EAHVHCYTHPQGSLTISAKKL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+  
Sbjct: 901  EAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSD 960

Query: 961  --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
                          FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYY+AINVQSV I
Sbjct: 961  AAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYAAINVQSVHI 1020

Query: 1021 PPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHY 1080
            PP ++EFHH NLEWINKEANEVHNRAKTLFAEV KALPLI  +ESGTEQEDFGVNEL++Y
Sbjct: 1021 PPSMIEFHHNNLEWINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELNNY 1080

Query: 1081 VQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFL 1140
            +QELEQI+NMEKE+FEVHFNEVL           SGE  +GQ  VDIF+INKLHMH+LFL
Sbjct: 1081 IQELEQIVNMEKENFEVHFNEVL-----------SGETTVGQSAVDIFQINKLHMHVLFL 1140

Query: 1141 SYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDND 1200
            SY WVQRL+Y   LRKK+S DSSSNDIP ++++ELDSLE+LV+FTL S RDEFF GFDND
Sbjct: 1141 SYIWVQRLNYVGTLRKKHSSDSSSNDIPAIEDKELDSLEELVEFTLASGRDEFFGGFDND 1200

Query: 1201 SLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIR 1260
            SLP ESK E+DL  GG SG+IS+SNE  REKN DLGG EDDLC S PVN  P DV I I+
Sbjct: 1201 SLPAESKREVDLRRGGNSGKISRSNEANREKNIDLGGQEDDLCFS-PVNFEPLDVGIGIQ 1260

Query: 1261 RANSEGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNA 1320
            RANS GEYPIM D S TLD VWKGVI PNN  FE+ S S TN A+LEPL+GQSD E+C A
Sbjct: 1261 RANSVGEYPIMTDFSGTLDEVWKGVIHPNNVIFED-SYSRTNFAILEPLAGQSDTERCTA 1320

Query: 1321 TLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFIS 1380
               GVET H LH+A+VLTRDDSVETITSST++PSSAY +KISLLNIPK +ISDYD AFIS
Sbjct: 1321 N--GVETTHFLHAALVLTRDDSVETITSSTSIPSSAYNTKISLLNIPKSDISDYDPAFIS 1380

Query: 1381 SFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDT 1440
            S+RELEKQ HLRLLMHVGSSDTV+PVYDEEPTSI+AYSLLMPEY  QMSE EK +E  DT
Sbjct: 1381 SYRELEKQSHLRLLMHVGSSDTVLPVYDEEPTSIVAYSLLMPEYIAQMSEKEKGMEASDT 1440

Query: 1441 SRSLPIIGSAKLSSLKTSDEEGSG----------TILSMPKTE-RQFEDRVSIAQNKHTR 1500
            +RSLPIIGSAK++ ++T DEEGS           TILSMP+TE RQFE+ +S  ++ HTR
Sbjct: 1441 ARSLPIIGSAKMNLIRTFDEEGSDAYKTLRSNEETILSMPRTESRQFENLLSFTKDLHTR 1500

Query: 1501 VSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFA 1560
            V FVNETPLGPVKYT+T +FAKRFEALRK CCP+ELDY+RSLSRCKRWGAQGGKSNVFFA
Sbjct: 1501 VCFVNETPLGPVKYTVTCYFAKRFEALRKMCCPTELDYIRSLSRCKRWGAQGGKSNVFFA 1560

Query: 1561 KTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGG 1620
            KTLDERFIIKQVTKIEL+SFIQFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+LKGG
Sbjct: 1561 KTLDERFIIKQVTKIELESFIQFARAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGG 1620

Query: 1621 KECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFL 1680
            KECK+D+LVMENLLFRHNVT+LYDLKGSSR+RYNSDTSGKN VLLDQNLIESMPTSPIFL
Sbjct: 1621 KECKVDVLVMENLLFRHNVTRLYDLKGSSRARYNSDTSGKNKVLLDQNLIESMPTSPIFL 1680

Query: 1681 GSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETW 1740
            GSKAKRLLERAVWNDTSFLAS+ VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW
Sbjct: 1681 GSKAKRLLERAVWNDTSFLASVDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETW 1740

Query: 1741 LKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDG 1748
            +KNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPS+TVSPSESHPDLCD+ 
Sbjct: 1741 VKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSTTVSPSESHPDLCDEN 1798

BLAST of Sed0006163 vs. NCBI nr
Match: KAG7020853.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1399/1798 (77.81%), Postives = 1527/1798 (84.93%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  LQDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+ KSINR
Sbjct: 121  SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS V+FD  CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L  DDD+DS +PG+W   PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK   FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DSSII NNV NL+S   L+  
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720

Query: 721  FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
                + +  +Q+ SI    +  +             DDDT+  SGK E SSQ  N I IS
Sbjct: 721  ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780

Query: 781  IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
             ++LDG+TQASN STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT 
Sbjct: 781  SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840

Query: 841  CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
            CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841  CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900

Query: 901  KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
            KL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+                FSNNA
Sbjct: 901  KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960

Query: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
            AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020

Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
            NEVHNRA+TLFAEV KALPLI   E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080

Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
            NEVL           SGE  +GQP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140

Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
            P SSSND+  LDER ++S+E+ V+FTL   +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200

Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
            QISQ NE EREK+ DLG LEDDLCLSP    +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260

Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
            AVWKGV+ PN+ T  +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT 
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320

Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
             DSVET TS  +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380

Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
            +DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440

Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
            EE +           TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500

Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
            FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560

Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
            F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620

Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
            T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680

Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
            ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740

Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778

BLAST of Sed0006163 vs. NCBI nr
Match: XP_023520487.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1401/1798 (77.92%), Postives = 1527/1798 (84.93%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  LQDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+S+ SSKSDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+GKSINR
Sbjct: 121  SLSSSSTSIGSSKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE ++ISQSD+YYC VNADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSECRNISQSDNYYCDVNADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS V+FD  CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L  DDD+DS +PG+W   PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK   FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DSSII NNV NL+S   L+  
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720

Query: 721  FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
                + +L +Q+ SI    +  +             DDDT+  SGK E SSQ  N I IS
Sbjct: 721  ASSTSLSLLKQDVSISDNNMLSSKFTSEANIRLDNKDDDTVPISGKGEVSSQLRNSIYIS 780

Query: 781  IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
             ++LDG+TQASN STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT 
Sbjct: 781  SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840

Query: 841  CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
            CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841  CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900

Query: 901  KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
            KL   F   E EGKIWMWHRCLKCPRTNGFPPATRR+I+                FSNNA
Sbjct: 901  KLPDFFLPGESEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960

Query: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
            AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020

Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
            NEVHNRA+TLFAEV KALPLI   E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080

Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
            NEVL           SGE  +GQP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140

Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
            P SSSND+  LDER ++S+E+ V+FTL   +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200

Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
            QISQ NE EREK+ DLG LEDDLCLSP    +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260

Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
            AVWKGV+ PN+ T  +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT 
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320

Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
             DSVET TS  +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380

Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
            +DTVVPVYDEEPTSIIAYSLL PEYN QM+EPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMAEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440

Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
            EE +           TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500

Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
            FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560

Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
            F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGGKECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGKECKLDILVMENLLFRHNV 1620

Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
            T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680

Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
            ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740

Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEESSEPEHSWDGKPYRYQSD 1778

BLAST of Sed0006163 vs. NCBI nr
Match: KAG6588292.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2711.8 bits (7028), Expect = 0.0e+00
Identity = 1399/1798 (77.81%), Postives = 1527/1798 (84.93%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  LQDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+ KSINR
Sbjct: 121  SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS V+FD  CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L  DDD+DS +PG+W   PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK   FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DSSII NNV NL+S   L+  
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720

Query: 721  FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
                + +  +Q+ SI    +  +             DDDT+  SGK E SSQ  N I IS
Sbjct: 721  ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780

Query: 781  IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
             ++LDG+TQASN STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT 
Sbjct: 781  SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840

Query: 841  CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
            CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841  CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900

Query: 901  KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
            KL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+                FSNNA
Sbjct: 901  KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960

Query: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
            AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020

Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
            NEVHNRA+TLFAEV KALPLI   E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080

Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
            NEVL           SGE  +GQP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140

Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
            P SSSND+  LDER ++S+E+ V+FTL   +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200

Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
            QISQ NE EREK+ DLG LEDDLCLSP    +P+DVEIS+RRANSEGEYPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGEYPIMADLSDTLD 1260

Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
            AVWKGV+ PN+ T  +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT 
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320

Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
             DSVET TS  +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380

Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
            +DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440

Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
            EE +           TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500

Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
            FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560

Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
            F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620

Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
            T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680

Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
            ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740

Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778

BLAST of Sed0006163 vs. NCBI nr
Match: XP_016899810.1 (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1397/1814 (77.01%), Postives = 1525/1814 (84.07%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENK+S+LVGL KSW+  WRS+  NV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKISDLVGLVKSWM-PWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+NFI A STD TN  EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61   RCGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            S+SSSS+SM S+KS YTC SA+S+I STPCS S QYYDPYCS  I DE D+LR+GK+INR
Sbjct: 121  SVSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S Y   GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN D GLNL+ 
Sbjct: 181  TTSLMTSSSSYC--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLER 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            EN  TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK++EPMDFQNNG+L
Sbjct: 241  ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKWAP-SFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
            WLPPEPEDEED+K L F+DD+D  +PG+W     SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301  WLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360

Query: 361  GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
            GHFRALV QLLEAE+LPIGD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361  GHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420

Query: 421  KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
            KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421  KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480

Query: 481  QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
            QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481  QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540

Query: 541  ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
            A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ  K S KTLMFFEGCPKPLCCT+L
Sbjct: 541  ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600

Query: 601  LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
            LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN 
Sbjct: 601  LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNI 660

Query: 661  DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
            DRSIS+IPGFTC      P PE   EIGQS KDAV ++D SII +NV NL+SLEP    +
Sbjct: 661  DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLL 720

Query: 721  EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
            E+N                          R+L+ +EYS+G MTIKE    D D +SSSGK
Sbjct: 721  ESNATSTSFSFLKQDFSTMLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSGK 780

Query: 781  DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
             EASS+  N  I +S +SLD +TQ+ NDSTSQ PE+   + VTTKSSREDFPLSPSDQQN
Sbjct: 781  REASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQN 840

Query: 841  ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
            ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900

Query: 901  HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
            HCYTHPQGSLTISAKKL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+      
Sbjct: 901  HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960

Query: 961  ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
                      FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020

Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
            +EFH +NLEWINKEANEVHNRAKTLF EV KALPLIS +ESG EQEDFG +EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQEL 1080

Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
            EQIINMEKE+FEVHFN+VL           SG   + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKVL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140

Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
            VQRL+Y A LRKK+SPD SSNDI  L+E+EL+SLE+LV+ TL S RDEFF G DNDSLP 
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPP 1200

Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
            ESK E  LG  G SG IS+ NE  REKN DLGG EDD CLS PVNS P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLS-PVNSEPVDVGIGIRRANS 1260

Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
             GEYPIM DLS TLDAVWKGVI PN+  FE+S  S TNSA+LEPL+GQSDMEKC A   G
Sbjct: 1261 VGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF-SRTNSAILEPLAGQSDMEKCTAN--G 1320

Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
            +ETAHLLHSA+VLT DDSVETIT+ST+MP SAY +KISLLNIPK +ISDY   FISS+RE
Sbjct: 1321 IETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380

Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
            LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEY  QMSEP K +E  DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSL 1440

Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
            P+IGS K++S+KT DEEGS           TILSMP+TE  QFED V + ++ HTRV FV
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFV 1500

Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
            NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560

Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
            ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A  T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620

Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
            +D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSG N VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKA 1680

Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
            KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740

Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
            GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1795

BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1133/1808 (62.67%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            M   ++K    V + KSWI   +S S N++RDFWMPD+SC VCYECD+QFT   RRHHCR
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFC+KC +N I +PS ++ +  E+ E+IRVCNYC+KQ  +      +G    +S I
Sbjct: 61   LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SL  SSS    S +  T  S+   I+ST   +     +P  S  ++   DS +S +  N 
Sbjct: 121  SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180

Query: 181  TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
             + R++   G+  D             G   G+ +DDD Y   +      +  +Q +DYY
Sbjct: 181  KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240

Query: 241  CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
             A+N DE  ++ G     ++  K    I   P  +  + L++E  D     EN      +
Sbjct: 241  GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300

Query: 301  FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
                  ++   E +DF+++G+LWLPPEPE+EED++  +  DDD D    G W    PS S
Sbjct: 301  GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360

Query: 361  LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
                ++  K +SS       K VVEGHFRALV QLLE ++LP+ +    +GWLDIIT LS
Sbjct: 361  FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
            WEAAT+LKPD SKSG MDP GYVKVKC+  G+RSES++VKG++CKKNVAH+RMT +I K 
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
            R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
            L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F        Q
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600

Query: 601  ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
             +K  AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
            GAS+ ELP ++P+TV LP  PS  +RSIS+IPGFT       P+ EL  E  ++  D   
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
            N  SS    +  +   D ++P         ER  +++ T+  K  E + + DD +  + +
Sbjct: 721  NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780

Query: 781  DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
                S      S+  D    L G+T+   D       +   N         +SS+ DF  
Sbjct: 781  SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840

Query: 841  SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
            S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ   C SC+
Sbjct: 841  SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
            MP+EAH+HCYTH QGSLTIS KKL  L   + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901  MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960

Query: 961  ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
                            FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961  SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020

Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
             +PP  L F++ N EW+ KE+ EV  +A+ LF EV +AL  IS    G   +    N++ 
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080

Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
              ++EL  ++   K++++    ++L+             V  GQP +DI  INKL   I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLII 1140

Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
            F SY W + L+ AA + + N  ++  N  P +  R + SLE+L    ++S          
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200

Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
            NDSL  ++ +E  L  G      S    +      D+G      C     P        V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260

Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
            E S  ++ A++E ++    DLSDTLDA W G      NG F   S + + +    P    
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320

Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
                  +  L+G E      S +      + +  T+   +PS ++Y   +K   LN  K 
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380

Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
             +++    ++SS+RELE +   RLL+ +G +D V+PVYD+EPTSIIAY+L   EY  QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440

Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
              +K+ +  D+  S  +  S  L SL +  +       S+   + Q    +  +   ++ 
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500

Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
            H R+SF +E P G VKY++T ++AK FEALR  CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560

Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
            FFAK+LD+RFIIKQVTK EL+SFI+F  AYF YL+E+  T  PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620

Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
            KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680

Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
            IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740

BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 882/1830 (48.20%), Postives = 1150/1830 (62.84%), Query Frame = 0

Query: 9    SELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCS 68
            SE+VGL KSW+  WRS    V+RDFWMPD+SCRVCYECD QFT I RRHHCR CGRVFC 
Sbjct: 12   SEIVGLIKSWL-PWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCG 71

Query: 69   KCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAISLSSSSS- 128
            KCT+N I    +D     ED E+IRVCNYCF+Q     +   DG    S+   LS+S S 
Sbjct: 72   KCTANSIPFAPSDLRTPREDWERIRVCNYCFRQ-----WEQGDGGPHVSNITELSTSPSE 131

Query: 129  -SMDSSKSDYTCQSALSNINSTP-----CSNSHQYYDPYCSFPITDEQDSLRSGKSINRT 188
             S+ SSK+  T  S+   + S P         H   D       + E    + GK  +R 
Sbjct: 132  TSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRR 191

Query: 189  TPRTAS---TSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNL 248
            +   A+       +     R DDE D+  YG Y++D ET H  ++++YY  +  + G+ +
Sbjct: 192  SSFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYN-GMGI 251

Query: 249  D--------GENICTK---GSAVIFDTPCPRSNEELSSED---SDENDKGSECD--FPAY 308
            D        GE    K   GS +I    C  S     SE      E+D   EC+   PA 
Sbjct: 252  DDVPCKHLGGETADQKSLSGSPLIHQ--CLESLIREGSEQFQKKSEHDGRDECEASSPAD 311

Query: 309  GMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK--LLFFDDDNDSRSPGKWA---PSFSLGS 368
               ++  EP+DF+NNG+LW+PPEPE+EED++   LF ++DN+  + G+W    PS S GS
Sbjct: 312  ISDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGS 371

Query: 369  GEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEA 428
            GEY  + R++EE++K  K VV+GHFRAL+ QLL+ E++ + D    + WL+IIT LSWEA
Sbjct: 372  GEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEA 431

Query: 429  ATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIF 488
            A +LKPDMSKSG MDP GYVKVKC+A G R +S++VKG++CKKNV ++RM+ +I K+R+ 
Sbjct: 432  ANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLL 491

Query: 489  VLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVA 548
            +LGG LEYQRV N +SS DTL+QQE DHLKMAVAKI A  PNILLVE+SVSR AQEYL+A
Sbjct: 492  ILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLA 551

Query: 549  KNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASK 608
            K+I++VLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ +       GQ  K
Sbjct: 552  KDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGK 611

Query: 609  TSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
               KTLM+FE CPKPL  T+LLRGA++DELKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 612  KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671

Query: 669  VPELPSKSPLTVILPHNPSNTDRSISSIPGFTC----PSPELNTEIGQSRKDAVPNKDSS 728
             PELP  SP+TV LP   ++ +RSIS++PGFT      SP + +     R ++VP  +  
Sbjct: 672  -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELL 731

Query: 729  IIRNNVANLDSLEP-------EFIEANRNLERQEYSIGTMTIKEAV--GADDDTISSSGK 788
                N++    + P       +  E N +     ++I ++ + + V    + D    S  
Sbjct: 732  STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNI-SLNLPDRVIESRNSDLSGRSVP 791

Query: 789  DEASSQRCNPISISIDSLDGSTQAS-------------------NDSTSQRPESPKAN-- 848
             +  + + NPI ++ ++ + S   S                    D+ S+   + + N  
Sbjct: 792  VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 851

Query: 849  -PVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLR 908
             P  T+S +E+FP SPSD Q+ILVSLS+R VWKGT C+R+HL RIKYYGSFD+PLGRFLR
Sbjct: 852  KPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLR 911

Query: 909  DNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLT-LFYREPEGKIWMWHRCLKC 968
            D+LFDQSYRC SC MPSEAHVHCYTH QGSLTIS KKL   L   E EGKIWMWHRCL+C
Sbjct: 912  DHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRC 971

Query: 969  PRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGR 1028
            PR NGFPPAT R+++                FSN+AAASRVA CGH LHRDCLRFYGFG 
Sbjct: 972  PRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGN 1031

Query: 1029 TVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNS 1088
             VACF Y+ I+V SV +PP IL F++ N +WI +E +EV  RA+ LF+EV  A+  I  +
Sbjct: 1032 MVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI--A 1091

Query: 1089 ESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQP 1148
            E G  +           + ELE+++  EK +FE +  ++L  E+   NE        GQP
Sbjct: 1092 EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREV---NE--------GQP 1151

Query: 1149 RVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVK 1208
             VDI E+ ++H  +LF SY W  RL  A+ L K  + D +        +RE +    L K
Sbjct: 1152 LVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDT--------KREENEKPPLAK 1211

Query: 1209 FTLESRRDEFFDGFDNDSLPLESKHEIDLG-NGGYSGQISQSNEMERE--KNFDLGGLED 1268
                    +     +  +  L +  E++L  +G  +G     N +++E   N DL   +D
Sbjct: 1212 -------SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKD 1271

Query: 1269 DLC-LSP----PVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQ-----PNN 1328
            D   +SP    P  S P + ++ +RR  S+G+  +M +LS TLDA W G  Q     P N
Sbjct: 1272 DGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTN 1331

Query: 1329 GTFEESSNSGTNSAVLEPLSG---QSDM-EKCNATLVGVETAHLLHSAIVLTRDDSVETI 1388
                   ++ +NS+   P+S      D+ E+ N   V    +  L S      +DSV   
Sbjct: 1332 NKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSV--- 1391

Query: 1389 TSSTAMPSSAYYSKIS---LLNIPK-PNISDYDHAFISSFRELEKQEHLRLLMHVGSSDT 1448
             S  ++P   +Y  I+   LL+  K     ++   +ISSFRE E Q   RLL+ VG +D 
Sbjct: 1392 -SWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDI 1451

Query: 1449 VVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEG 1508
            VVPVYD+EPTS+IAY+L+ PEY  Q S   ++L    +  ++P      +     S+   
Sbjct: 1452 VVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNGSV 1511

Query: 1509 SGTILSMPKT-ERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1568
              +ILS+  +      D +S  +  H RVS+  +  LG VKYT+T ++AKRFEALR  C 
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571

Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1628
            PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK EL+SFI+FA AYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631

Query: 1629 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1688
            E+  T  PTCLAK++GIYQV++K LK GKE K+D+L+MENLLF   V +LYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691

Query: 1689 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1733
            YN D+SG N VLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLA   VMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751

BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-285
Identity = 651/1799 (36.19%), Postives = 958/1799 (53.25%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNS----ENVARDFWMPDRSCRVCYECDSQFTFIKRR 60
            MG P+  L +L+   +SWI+S  S+S     +  +DF +     ++C++C    T +++ 
Sbjct: 1    MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60

Query: 61   HHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYC---FKQLGREPFALQDGD 120
            + C  CG  +C  C+                +  K+++C  C    ++L  + +      
Sbjct: 61   YCCLSCGSCWCKSCSDT--------------EESKMKLCRECDAEVRELRVKSYDKVHPR 120

Query: 121  KATSSAISLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLR 180
             +     SL++ S S+ SS     C+    N+ S  C  S             +E+++  
Sbjct: 121  DSPDPPSSLATESESLASSLEIRDCR----NMASIRCYPSR-----------GEEEEARY 180

Query: 181  SGKSINRTTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVN-- 240
             GK +   +      S     G      E     +    S   + H S   + +  +   
Sbjct: 181  CGKQLLSPSSDNYQDSSDIESGSVSARHE----LFSCKSSAGSSPHDSPLRNNFSPLGRF 240

Query: 241  ADEGLNLDGENICT---KGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKN 300
                 +L    +C+       ++ D         L  ED +E              ++K 
Sbjct: 241  VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------EDKL 300

Query: 301  NEPMDFQNNGVLWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG---SGEYPMKG 360
             +P+DF+NNG +W PP PEDE DD +  +F  D++    G  A  FSL    S   P K 
Sbjct: 301  QQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIPTKE 360

Query: 361  RSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPD 420
            +  E   +  + VV  HFRALV +LL  E+L   D  S   WLDI+T L+W+AA  +KPD
Sbjct: 361  KLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPD 420

Query: 421  MSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALE 480
                G MDP  YVK+KCVA G ++ESI+++GI+C KN+ HKRM  +    R+ +L G+LE
Sbjct: 421  TRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLE 480

Query: 481  YQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVL 540
            YQRV   ++S +TL+QQE +H+K  +AKI++  PN+LLVE+S S +AQ+YL+ K I++VL
Sbjct: 481  YQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVL 540

Query: 541  NIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVE-TFEVHDTGDGQASKTSAKTL 600
            N+KRSLL+RIA CTGA + P++D +++ +LG+C+ FR E   E H+ G+ Q+++  ++TL
Sbjct: 541  NVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTL 600

Query: 601  MFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPS 660
            M+FEGCP+ L CTV+LRG+ ++ELKKVKHV+QYA FAAYHL+LET FLADEGAS+P++  
Sbjct: 601  MYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRL 660

Query: 661  KSPLTV--------------ILPHNPSNTD-----RSISSIPGFTCPSPELNTEIGQSR- 720
            K P  V              ++  +P+ TD      + +     T P PE   E+ +S  
Sbjct: 661  KQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPE--HEVCESLC 720

Query: 721  KDAVPNK---DSSIIRNNVANLDSLEPEFIEANRNLERQEYSIGTMT-IKEAVGADDDTI 780
            +D  P +    SS +    +  D+L  +F     NL  + YS   +  + E        I
Sbjct: 721  EDFDPTQIFPPSSEVETEQS--DTLNGDFA---NNLVTRSYSSNQLNDLHEPTLCLSSEI 780

Query: 781  SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
              +   + S +  N          G  +  N   + + + P+           ++  +  
Sbjct: 781  PETPTQQPSGEEDN----------GRGEEENQLVNPQ-DLPQHESFYEDDVSSEYFSAAD 840

Query: 841  DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
              Q+ILVS S+RCV K + C+R+ LLRIK+YGSFD+PLGR+L+D+LFD++  C SC    
Sbjct: 841  SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900

Query: 901  EAHVHCYTHPQGSLTISAKKLLTL-FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
            +AHV CY+H  G+LTI+ ++L ++    E +GKIWMWHRCL+C   +G PPATRR+++  
Sbjct: 901  DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960

Query: 961  --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
                          FSN+A A+RVA CGH L RDCLRFYGFG  VA F YS IN+ +VL+
Sbjct: 961  AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020

Query: 1021 PPPILEF--HHNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNEL-S 1080
            PP +LEF  H   EWI  EA E+  + +T++ E+   L  +    S  E E     +L S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080

Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
              +  ++Q++  EK++++     +    + +            Q  +DI E+N+L   ++
Sbjct: 1081 RIIGLIDQLVK-EKDEYDDALQPIFEENLQI------------QGSLDILELNRLRRALM 1140

Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
              ++ W  +L    +  KK S   + +D                      R  E  D   
Sbjct: 1141 IGAHAWDHQLYLLNSQLKKASVFKTGDD-------------------NAPRNPEMHDPPK 1200

Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEIS 1260
             D    E   E D           + +  + E N D              N  P ++   
Sbjct: 1201 IDRRMQEGSDERD-----------EQSHTDSEANGD--------------NKDPENIP-- 1260

Query: 1261 IRRANSEGEYPIMADLSDTLDAVWKGVIQ--PNNGTFEESSNSGTNSAVLEPLSGQSDME 1320
                 S G       LS+ +D+ W G  Q      T  E+      ++ L  L+    ++
Sbjct: 1261 -----SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQ 1320

Query: 1321 KCNATL-------VGVETAHLLHSAI-VLTRDDSVETITSSTAMPSSAYYSKISLLNIPK 1380
              ++ +        G+  + L  S +           +           YS++  L + K
Sbjct: 1321 SFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQK 1380

Query: 1381 PN-ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQ 1440
             + I      +ISS  ++     + L+   G +D VVPVYD++P S+++Y++   EY   
Sbjct: 1381 LDLIVGSAPTYISSASQMADGARM-LIPQRGLNDIVVPVYDDDPASVVSYAINSKEYK-- 1440

Query: 1441 MSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG----TILSMPKTERQF------- 1500
                E  +  G       +  S+  S+L   + E S       LSM     Q        
Sbjct: 1441 ----EWIVNKG-------LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQ 1500

Query: 1501 EDRVS--IAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRC 1560
            +DR S  +  +   R S  +    G VK+++T +FA +F+ LRK CCPSE+D+VRSLSRC
Sbjct: 1501 DDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRC 1560

Query: 1561 KRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAK 1620
            +RW AQGGKSNV+FAK+LDERFIIKQV K EL SF  FA  YF YL E+  +  PTCLAK
Sbjct: 1561 QRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAK 1620

Query: 1621 VVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLL 1680
            ++GIYQVS K+ KGGKE K+D++VMENL +   ++++YDLKGS+RSRYN +TSG + VLL
Sbjct: 1621 ILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLL 1648

Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGII 1715
            D NL+E++ T PIFLGSKAKR LERA+WNDT+FLAS+ VMDYSLLVG DEE++ELV+GII
Sbjct: 1681 DMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGII 1648

BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 7.1e-179
Identity = 469/1464 (32.04%), Postives = 726/1464 (49.59%), Query Frame = 0

Query: 300  LWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG--SGEYPMKGRSSEEYRKTAKY 359
            +W PPEPE+ ED+   +F DDD+D     KW  +  LG  S E   K +  EE R+    
Sbjct: 95   VWEPPEPENPEDEVDGVFADDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLE 154

Query: 360  VVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGY 419
              +  F+ +V QL+++    I +      W +I+  L WEAA++LKP +     +DP+ Y
Sbjct: 155  EADSKFKFIVSQLIKSAGFSIEE---SGYWFEIVARLCWEAASMLKPAIDGKS-VDPTEY 214

Query: 420  VKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLD 479
            +KVKC+A G   +S + KG++ KK+ A K M  +    RI ++ G L +   I+  SSL 
Sbjct: 215  IKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPRIMLVEGVLGHP--ISGFSSLQ 274

Query: 480  TLMQQE---LDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLER 539
            ++ Q     L ++K  V  I+A  P+++LVE+SVSR  Q+ ++ K +T+V ++K   L+R
Sbjct: 275  SVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQR 334

Query: 540  IASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASKTSAKTLMFFEGCPKPL 599
            I+ C G+ I+ +VD ++S KL +CD+FR+E         G++ K   KTLMF EGCP  L
Sbjct: 335  ISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRL 394

Query: 600  CCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILP- 659
             CT+LL+G   + LKKVK VVQY+   AYHL LE  FLAD       + +K   + ++  
Sbjct: 395  GCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEI 454

Query: 660  --HNPSNTDRSISS----IP---GFTCPSPELNTEIGQSRKDAVPNKDSSIIRNNVANLD 719
               +PS + R   S    IP   GF   + ++N   G++  + V   +S    ++V + +
Sbjct: 455  ENFSPSPSPRESPSEAVDIPVSNGFDEQTIQIN---GEADGEKVGTWESD--GDHVFSHE 514

Query: 720  SLEPEFIEANRNLE-RQEYSIGTMTIKEA--VGADDDTISSSGKDEASSQRCNPISISID 779
               P       +L  R    +G +   E+  V  D D  ++S  D            SI 
Sbjct: 515  PYNPVIFTGFSSLSARLSKYLGFVQNPESVPVSVDTDVSTTSNLD------------SIR 574

Query: 780  SLDGSTQASNDSTS------QRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVW 839
              +  T   N+         + P +  ++    KS  E+   S  + Q+ILV +S R   
Sbjct: 575  ESEEDTAEKNEDKQPLLLDPELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNAL 634

Query: 840  KGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLT 899
            +G  CD+ H   IK+Y  FD PL +FLRD +F+Q   C +C    EAH++ Y H    LT
Sbjct: 635  RGIMCDQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLT 694

Query: 900  ISAKKLLTL--FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII---------------- 959
            I  K++        E +GKIWMW RC KC   N    +T+R++I                
Sbjct: 695  IQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELS 754

Query: 960  FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHHNLE-- 1019
            FS     +R + CGH    D L F+G G  VA   YS +   +V +PP  LE    ++  
Sbjct: 755  FSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAG 814

Query: 1020 WINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKE 1079
            W+ KE   V  +  +LF +   A   +    S     D         +  +E+++  E+ 
Sbjct: 815  WLEKEFQTVFTKGISLFED---AAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERC 874

Query: 1080 DFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAA 1139
             FE +          +KN     +  I      +  +N++   +L  +  W  RL  +  
Sbjct: 875  IFEEN----------IKNSFDKAK-TIDDVSHRLLRLNRMRWELLLQALIWNYRLQ-SLV 934

Query: 1140 LRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSL--PLESKHEID 1199
            L  +  P   S+D   + E+ L ++ +    T     ++  D   N  +  PL    +I 
Sbjct: 935  LSDRLLP---SSDETKIYEQGLKTVSE-AGMTRYENDNKVSDSGSNGGIDTPLVEHKDIP 994

Query: 1200 LGNG--GYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYP 1259
            +     G + Q+++S   E  ++  L     D   SP  N     + +++   N +    
Sbjct: 995  IAGASVGDNDQMAESYVPEDNESQTLCSSSPD-TTSPINNHFDTHLAVNVHSTNGQEADK 1054

Query: 1260 IMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAH 1319
             +    ++LD              E S+++G +    +        E  +  +V +E  +
Sbjct: 1055 SIPVTGESLDD-------------EVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEY 1114

Query: 1320 LLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQE 1379
            LL    V                                   +++    + +  ++  +E
Sbjct: 1115 LLKFEYV-----------------------------------NNFTQENLQTVNQIITEE 1174

Query: 1380 HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGS 1439
              RL + +   D +V  Y++E +S+IA +L     ++   E +K L       S  I GS
Sbjct: 1175 SSRLRISLRDDDFIVSDYEDELSSLIACALA----HLNNEESKKPL-------SRCIHGS 1234

Query: 1440 AKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFF 1499
              L     ++++   T   + +   +  +R+         V+F +   +G  KY+I   +
Sbjct: 1235 --LQGFLDNNQDSKQTDRDVSRFSSESTNRLETLPPPEVLVTFGSVKSVGKPKYSIVSLY 1294

Query: 1500 AKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSF 1559
            A  F  LRKRCC SELDY+ SLSRCK W A+GGKS   FAKTLD+RFI+K++ K E +SF
Sbjct: 1295 ADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESF 1354

Query: 1560 IQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLK-GGKECKIDILVMENLLFRHNV 1619
            + FA+ YF Y+ ++      TCLAKV+GI+QV+ +  K GGKE + D++VMENL F   V
Sbjct: 1355 VTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKV 1414

Query: 1620 TQLYDLKGSSRSRYN-SDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1679
            T+ YDLKG+  +R+  +  +G++ VLLDQN +  M  SP+++   +K+ L+RAV+NDTSF
Sbjct: 1415 TRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSF 1450

Query: 1680 LASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVI 1713
            L SI+VMDYSLLVG+D+E  ELV GIIDY+RQYTWDK LETW+K+S ++  PKN  PTVI
Sbjct: 1475 LTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVV--PKNVQPTVI 1450

BLAST of Sed0006163 vs. ExPASy Swiss-Prot
Match: Q9Z1T6 (1-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve PE=1 SV=3)

HSP 1 Score: 364.0 bits (933), Expect = 9.5e-99
Identity = 494/2104 (23.48%), Postives = 801/2104 (38.07%), Query Frame = 0

Query: 31   RDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSE 90
            + +WMPD  C+ CY+C  +FT  +RRHHCR CG++FCS+C +  I       T       
Sbjct: 152  KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTG------ 211

Query: 91   KIRVCNYCFK------------QLGREPFALQDG-------------------------- 150
             +R C YC K             +G +  AL D                           
Sbjct: 212  DLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIF 271

Query: 151  ---DKATSSAISLSSSSSSM---------DSSKSDYTCQSA-LSNIN----------STP 210
               D A  S I   SS+S++         D+ KS    +SA ++N++          S  
Sbjct: 272  LEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYE 331

Query: 211  CSNSHQYYDPYCSFPITDEQDSL-----------------RSGKSIN------RTTPR-- 270
             S S Q    Y     T+++  +                  SG          RT P   
Sbjct: 332  TSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCI 391

Query: 271  -------------------------TASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKH 330
                                      A   G + D     D    D  Y  YR    T+ 
Sbjct: 392  VGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRD-EYALYRPLQSTEF 451

Query: 331  ISQSDDYYCAVNADEGL------------NLDGENICTKGSAVIFDTPCPRSNEELSSED 390
                     +VN+ EG             + D E I  +G   + ++  P     +SS  
Sbjct: 452  SETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQ 511

Query: 391  SDENDKGSECDFPAYGMQNKN---NEPMDFQNNGVLWLPPEPEDEED-------DKLLFF 450
            S  +   +        + N N    +P  + +     +PP P D+++        + L  
Sbjct: 512  STVDSDSAASISLNVELDNVNFHIKKPSKYPH-----VPPHPADQKEYLVSDTGGQQLSI 571

Query: 451  DD--------DNDSRSPGKWAPSFSLGSGEYPMKGRSSEEYRKTAKYVV----EGHFRAL 510
             D        +       K  P   LG     ++    E   K A   +      H  AL
Sbjct: 572  SDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMAL 631

Query: 511  VGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMS-KSGPMDPSGYVKVKCVAG 570
            + QLL+ E L          W DII  L  +    ++PD+  +   MD   +V +K + G
Sbjct: 632  LQQLLQNESL-------SSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPG 691

Query: 571  GKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEY-QRVINHVSSLDTLMQQEL 630
            GK+ +S++V G +C KN+AHK+M   I   +I +L  ++EY  R     + +D ++ QE 
Sbjct: 692  GKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQER 751

Query: 631  DHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTGASII 690
            + LK  V +I    P ++LVE++VSR AQ+ L+   IT+V+N+K  +LERI+  T   ++
Sbjct: 752  EFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLV 811

Query: 691  PTVDHVASL-KLGYCDAFRVETFEVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVLLRGA 750
             ++D + +   LG C  F ++ F        Q      KTLMFFEGCP+ L CT+ LRG 
Sbjct: 812  VSMDQLLTKPHLGTCHKFYMQIF--------QLPNEQTKTLMFFEGCPQHLGCTIKLRGG 871

Query: 751  SKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNTDRSI 810
            S  EL +VK ++ +    AYH  LE  FL DE A  P L  +SP   +L         S 
Sbjct: 872  SDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTL-MQSPSFHLLTEGRGEEGASQ 931

Query: 811  SSIPGFTCPSPELNTEIGQSRKDAVPNKDSSIIRN-NVANLDSLEPEFI-EANRNLERQE 870
              + G + P         +  ++A+ ++DS+++ +  V     L+ + I +A  +L+ Q+
Sbjct: 932  EQVSGSSLPQDP------ECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQD 991

Query: 871  YSIGT---------------------MTIKEAVGAD------------------------ 930
            Y+  T                     M  ++AVG +                        
Sbjct: 992  YTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDP 1051

Query: 931  --DDTISSSGKDEASSQ--------------------------------------RCN-- 990
              DDT     ++  SS+                                      RC+  
Sbjct: 1052 LQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTR 1111

Query: 991  ---PISI--------------------------SIDSLDGSTQASN-------------- 1050
               P  I                           +  ++GS QA +              
Sbjct: 1112 DYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRI 1171

Query: 1051 -----DSTS------------QRPESPKANPVT----------------TKSSRED---- 1110
                 DS +             R +S   +P                  T+S  E     
Sbjct: 1172 AEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIP 1231

Query: 1111 ----FP-----LSPSDQQNILVSLSTRCVWKG---TTCDRAHLLRIKYYGSFDEPLGRFL 1170
                +P     L+P++ Q + V  S+         + C    ++ +++YG  D  LG FL
Sbjct: 1232 SDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFL 1291

Query: 1171 RDNLFDQSYRCHS--CSMPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCL 1230
                F  SY+C S  C  P   H+  + H QG + I  K+L +      +  I  +  C 
Sbjct: 1292 ERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSP-VPGYQHTILTYSWCR 1351

Query: 1231 KCPRTNGFPPATRRIIIFSNNAAASRVA-------------------GCGHFLHRDCLRF 1290
             C +          ++  SN + +   A                    CGH +H D  ++
Sbjct: 1352 ICKQVT-------PVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQY 1411

Query: 1291 YGFGRTVACFYYSAINVQSVLIPPPILEFHHNLEWINKEANEVHNRAKTLFAEVYKALPL 1350
            + + + VA F YS I +  V +P P +       +I ++A         L   + + L  
Sbjct: 1412 FSYNQMVASFSYSPIRLLEVCVPLPKI-------FIKRQA--------PLKVSLLQDL-- 1471

Query: 1351 ISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVA 1410
                      +DF       Y+   E++ +++ + F     E +  +I  + E+  GE  
Sbjct: 1472 ----------KDFFQKVSQVYLAVDERLASLKTDTFSKTREEKME-DIFAQKEMEEGEFK 1531

Query: 1411 IGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLE 1470
                                    W +++         ++P    +    L  ++    E
Sbjct: 1532 -----------------------NWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCE 1591

Query: 1471 QLVKFTLESRRDEFFD---GFDNDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLG 1530
             L  +   SR  + F    G    S+P               G++ Q  E +        
Sbjct: 1592 VLQAW--NSRLQDLFQQEKGRKRPSVPPS------------PGRLRQGEESKIN------ 1651

Query: 1531 GLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQPNNGTFEES 1590
                       +++ PR++   +     E  +        +  +    +  P     E+ 
Sbjct: 1652 ----------AMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQV 1711

Query: 1591 SNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSA 1650
                T+   L+  SG  D          V   HLL S     ++ S      +  +P ++
Sbjct: 1712 VGGPTD---LDSASGSED----------VFDGHLLGSTDSQVKEKSTMKAIFANLLPGNS 1771

Query: 1651 YYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIA 1710
            Y         P P   D D  ++         EH R+          + V ++EP+SIIA
Sbjct: 1772 YN--------PIPFPFDPDKHYL-------MYEHERV---------PIAVCEKEPSSIIA 1831

Query: 1711 YSLLMPEYNVQMSEPEKA------------------------------LEPGDTSRSL-- 1713
            ++L   EY   + E  KA                              +  G T+R++  
Sbjct: 1832 FALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEA 1891

BLAST of Sed0006163 vs. ExPASy TrEMBL
Match: A0A1S4DVR6 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103487265 PE=4 SV=1)

HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1397/1814 (77.01%), Postives = 1525/1814 (84.07%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENK+S+LVGL KSW+  WRS+  NV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKISDLVGLVKSWM-PWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+NFI A STD TN  EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61   RCGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            S+SSSS+SM S+KS YTC SA+S+I STPCS S QYYDPYCS  I DE D+LR+GK+INR
Sbjct: 121  SVSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S Y   GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN D GLNL+ 
Sbjct: 181  TTSLMTSSSSYC--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLER 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            EN  TKGS V FDT CPR NEE SSEDSDENDKGSECDFP Y MQNK++EPMDFQNNG+L
Sbjct: 241  ENTSTKGSPVNFDTQCPRGNEE-SSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKWAP-SFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
            WLPPEPEDEED+K L F+DD+D  +PG+W     SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301  WLPPEPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360

Query: 361  GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
            GHFRALV QLLEAE+LPIGD P+ DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361  GHFRALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420

Query: 421  KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
            KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421  KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480

Query: 481  QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
            QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481  QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540

Query: 541  ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
            A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ  K S KTLMFFEGCPKPLCCT+L
Sbjct: 541  ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600

Query: 601  LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
            LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN 
Sbjct: 601  LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNI 660

Query: 661  DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
            DRSIS+IPGFTC      P PE   EIGQS KDAV ++D SII +NV NL+SLEP    +
Sbjct: 661  DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLL 720

Query: 721  EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
            E+N                          R+L+ +EYS+G MTIKE    D D +SSSGK
Sbjct: 721  ESNATSTSFSFLKQDFSTMLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSGK 780

Query: 781  DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
             EASS+  N  I +S +SLD +TQ+ NDSTSQ PE+   + VTTKSSREDFPLSPSDQQN
Sbjct: 781  REASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQN 840

Query: 841  ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
            ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900

Query: 901  HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
            HCYTHPQGSLTISAKKL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+      
Sbjct: 901  HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960

Query: 961  ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
                      FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020

Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
            +EFH +NLEWINKEANEVHNRAKTLF EV KALPLIS +ESG EQEDFG +EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQEL 1080

Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
            EQIINMEKE+FEVHFN+VL           SG   + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKVL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140

Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
            VQRL+Y A LRKK+SPD SSNDI  L+E+EL+SLE+LV+ TL S RDEFF G DNDSLP 
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPP 1200

Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
            ESK E  LG  G SG IS+ NE  REKN DLGG EDD CLS PVNS P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNETNREKNIDLGGQEDDFCLS-PVNSEPVDVGIGIRRANS 1260

Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
             GEYPIM DLS TLDAVWKGVI PN+  FE+S  S TNSA+LEPL+GQSDMEKC A   G
Sbjct: 1261 VGEYPIMTDLSGTLDAVWKGVIHPNSLIFEDSF-SRTNSAILEPLAGQSDMEKCTAN--G 1320

Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
            +ETAHLLHSA+VLT DDSVETIT+ST+MP SAY +KISLLNIPK +ISDY   FISS+RE
Sbjct: 1321 IETAHLLHSALVLTSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380

Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
            LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEY  QMSEP K +E  DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSL 1440

Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
            P+IGS K++S+KT DEEGS           TILSMP+TE  QFED V + ++ HTRV FV
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFV 1500

Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
            NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560

Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
            ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A  T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620

Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
            +D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSG N VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKA 1680

Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
            KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740

Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
            GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1795

BLAST of Sed0006163 vs. ExPASy TrEMBL
Match: A0A6J1EZX4 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438136 PE=4 SV=1)

HSP 1 Score: 2709.9 bits (7023), Expect = 0.0e+00
Identity = 1397/1798 (77.70%), Postives = 1527/1798 (84.93%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  +QDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+ KSINR
Sbjct: 121  SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS V+FD  CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L  DDD+DS +PG+W   PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK   FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DSSII NNV NL+S   L+  
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSN 720

Query: 721  FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
                + +  +Q+ SI    +  +             DDDT+  SGK E SSQ  N I IS
Sbjct: 721  ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780

Query: 781  IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
             ++LDG+TQASN STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT 
Sbjct: 781  SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840

Query: 841  CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
            CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841  CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900

Query: 901  KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
            KL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+                FSNNA
Sbjct: 901  KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960

Query: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
            AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEA 1020

Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
            NEVHNRA+TLFAEV KALPLI   E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1080

Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
            NEVL           SGE  +GQP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNS
Sbjct: 1081 NEVL-----------SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140

Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
            P SSSND+  LDER ++S+E+ V+FTL   +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200

Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLD 1260
            QISQ NE EREK+ DLG LEDDLCLSP    +P+DVEIS+RRANSEG+YPIMADLSDTLD
Sbjct: 1201 QISQLNETEREKDIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGDYPIMADLSDTLD 1260

Query: 1261 AVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTR 1320
            AVWKGV+ PN+ T  +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT 
Sbjct: 1261 AVWKGVMHPNSAT-SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1320

Query: 1321 DDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGS 1380
             DSVET TS  +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS
Sbjct: 1321 VDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS 1380

Query: 1381 SDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSD 1440
            +DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT D
Sbjct: 1381 TDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCD 1440

Query: 1441 EEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGF 1500
            EE +           TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT +
Sbjct: 1441 EEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCY 1500

Query: 1501 FAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQS 1560
            FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+S
Sbjct: 1501 FAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELES 1560

Query: 1561 FIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNV 1620
            F QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNV
Sbjct: 1561 FFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNV 1620

Query: 1621 TQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680
            T+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL
Sbjct: 1621 TRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFL 1680

Query: 1681 ASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVIS 1740
            ASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVIS
Sbjct: 1681 ASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVIS 1740

Query: 1741 PHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            PHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 PHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778

BLAST of Sed0006163 vs. ExPASy TrEMBL
Match: A0A6J1KWW8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111497876 PE=4 SV=1)

HSP 1 Score: 2699.8 bits (6997), Expect = 0.0e+00
Identity = 1399/1799 (77.77%), Postives = 1521/1799 (84.55%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  LQDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            S SSSS+S+ S+KSDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+GKSINR
Sbjct: 121  SPSSSSTSIGSTKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC  NADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDANADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS VIFD  C R +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVIFDNQCQRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L FDDD+DS +PG+W   PS SL  GE P+KG SSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLFDDDDDSCAPGEWGYVPS-SLDIGECPVKGSSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKR ESIIVKGIICKKNVAH+RMTREISK R FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRCESIIVKGIICKKNVAHRRMTREISKPRFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K S KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKSTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV L HNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALLHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDS---LEPE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DS II NNV NL+S   L+  
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSPIISNNVVNLNSLRLLKSN 720

Query: 721  FIEANRNLERQEYSIGTMTIKEAV----------GADDDTISSSGKDEASSQRCNPISIS 780
                + +  +Q+ SI    +  +             DDDT+  SGK E SSQ  N I IS
Sbjct: 721  ASSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYIS 780

Query: 781  IDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTT 840
             ++LDG+TQASN STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT 
Sbjct: 781  SNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTI 840

Query: 841  CDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAK 900
            CDRAHLLRIKYYGSFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAK
Sbjct: 841  CDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAK 900

Query: 901  KLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNA 960
            KL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+                FSNNA
Sbjct: 901  KLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNA 960

Query: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEA 1020
            AASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEA
Sbjct: 961  AASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPALIEFYHNNLEWINKEA 1020

Query: 1021 NEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHF 1080
            NEVHNRA+TLFA V KALPLI  +E G+EQED GVNEL+HY+QELEQII MEKEDFEVHF
Sbjct: 1021 NEVHNRAETLFAAVCKALPLIPKNELGSEQEDVGVNELNHYIQELEQIIIMEKEDFEVHF 1080

Query: 1081 NEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNS 1140
            NEVL           SGE  + QP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNS
Sbjct: 1081 NEVL-----------SGETTVSQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNS 1140

Query: 1141 PDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSG 1200
            P SSSND+  LDER ++S+E+ VKFTL   +D+FF G DNDSLPLESKHEIDLG GG SG
Sbjct: 1141 PYSSSNDLLTLDERGIESVEEFVKFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSG 1200

Query: 1201 QISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANSE-GEYPIMADLSDTL 1260
            QISQ NEMEREKN DLG LEDDLCLSP    +P+DVEIS+RRANSE GEYPIMADLSDTL
Sbjct: 1201 QISQLNEMEREKNIDLGVLEDDLCLSP---VMPQDVEISMRRANSEGGEYPIMADLSDTL 1260

Query: 1261 DAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLT 1320
            DAVWKGV+ PN+ T  ESS S TNSA+LEPL  QSDMEKCNA LVG ETA LLHSA+VLT
Sbjct: 1261 DAVWKGVMHPNSVT-SESSYSQTNSAILEPLVVQSDMEKCNANLVGAETARLLHSALVLT 1320

Query: 1321 RDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVG 1380
              DSVET TS  +MPSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVG
Sbjct: 1321 MVDSVETSTSLVSMPSSAYSTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTS 1440
            S+DTVVPVYDEEPTSIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT 
Sbjct: 1381 SNDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTC 1440

Query: 1441 DEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITG 1500
            DEE +           TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT 
Sbjct: 1441 DEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITC 1500

Query: 1501 FFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQ 1560
            +FAKRFEALRK+CCPSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+
Sbjct: 1501 YFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHN 1620
            SF QFA AYF+YLSEA RT CPTCLAKVVG+YQVSSK+LKGGKECK+DILVMENLLFRHN
Sbjct: 1561 SFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKECKLDILVMENLLFRHN 1620

Query: 1621 VTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
            VT+LYDLKGSSRSRYNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVI 1740
            LASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            SPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKRYRYQSD 1779

BLAST of Sed0006163 vs. ExPASy TrEMBL
Match: A0A0A0M3A5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 2697.5 bits (6991), Expect = 0.0e+00
Identity = 1387/1814 (76.46%), Postives = 1526/1814 (84.12%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENK+S+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCYECDSQFTFI RRHHCR
Sbjct: 1    MGHPENKISDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCS+CT+NFI APSTD+TN  EDSEKIRVCNYCFKQ G+EP +LQDGDKATSSA+
Sbjct: 61   RCGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSAL 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+SM S+KS YTC SA+S+I STPCS   QYYDPYCS  + DE D+LR+GK+INR
Sbjct: 121  SLSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  GYYR DDEDDDYYYG YRSDSE++HISQSDDYYCAVN DEG NL+ 
Sbjct: 181  TTSLMTSSSSYY--GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLER 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            EN  TKGS V FDT  PR NEE SSEDSDENDKGSECDFP+Y MQNKN+EPMDFQNNG+L
Sbjct: 241  ENTSTKGSPVNFDTQSPRGNEE-SSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKWA-PSFSLGSGEYPMKGRSSEEYRKTAKYVVE 360
            WLPPEPEDEED+K L F+DD+D  +PG+W     SL SGE PMKG+SSE+Y+KTAK VVE
Sbjct: 301  WLPPEPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVE 360

Query: 361  GHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVKV 420
            GHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVKV
Sbjct: 361  GHFRALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKV 420

Query: 421  KCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTLM 480
            KCVAGGKRSESIIVKGIICKKNVAH+RMTR ISK R FVLGGALEYQRV NH+SS DTL+
Sbjct: 421  KCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLL 480

Query: 481  QQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCTG 540
            QQELDHLKMAVAKIDAHHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CTG
Sbjct: 481  QQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTG 540

Query: 541  ASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTVL 600
            A+IIPTVDHVASLKLGYCDAFRVETF E HD+GDGQ  K S KTLMFFEGCPKPLCCT+L
Sbjct: 541  ANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTIL 600

Query: 601  LRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSNT 660
            LRGAS DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN 
Sbjct: 601  LRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNV 660

Query: 661  DRSISSIPGFTC------PSPELNTEIGQSRKDAVPNKDSSIIRNNVANLDSLEP--EFI 720
            DRSIS+IPGFTC      P PE   EIGQS KDAV ++DSSII +NV NL+SLEP    +
Sbjct: 661  DRSISAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLL 720

Query: 721  EAN--------------------------RNLERQEYSIGTMTIKEAVGADDDTISSSGK 780
            E+N                          R+L  +EYS+G M IKE  G DDD +SSSGK
Sbjct: 721  ESNATSPSFSFLKQDFSTMISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSGK 780

Query: 781  DEASSQRCN-PISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPSDQQN 840
             EASS+  N  + ++ +SLD +TQ+ N+STSQ PE+   + VTTKSSREDFPLSPSDQQN
Sbjct: 781  REASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQN 840

Query: 841  ILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHV 900
            ILVSLSTRCVWKGT CDRAHLLRIKYYGSFD+PLGRFLRDNLFDQ+YRCHSC MPSEAHV
Sbjct: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900

Query: 901  HCYTHPQGSLTISAKKLLTLFY-REPEGKIWMWHRCLKCPRTNGFPPATRRIII------ 960
            HCYTHPQGSLTISAKKL   F   EPEGKIWMWHRCLKCPRTNGFPPATRR+I+      
Sbjct: 901  HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960

Query: 961  ----------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLIPPPI 1020
                      FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACF+Y+AINVQSV IPP +
Sbjct: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020

Query: 1021 LEFH-HNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSHYVQEL 1080
            +EFH +NLEWINKEANEVHNRAKTLF EV KALPLI  +ESG EQEDFGV+EL++Y+QEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQEL 1080

Query: 1081 EQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILFLSYTW 1140
            EQIINMEKE+FEVHFN++L           SG   + QP VDIFEINKLHMHILFLSY W
Sbjct: 1081 EQIINMEKENFEVHFNKLL-----------SGGTTVSQPTVDIFEINKLHMHILFLSYIW 1140

Query: 1141 VQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDNDSLPL 1200
            VQRL+Y A LRKK+SPD SSNDI +L E+EL+SLE+LV+ TL S RDEFF G DNDSLP 
Sbjct: 1141 VQRLNYVATLRKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPA 1200

Query: 1201 ESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEISIRRANS 1260
            ESK E  LG  G SG IS+ N+ +REKN DLGG EDD CLS PVN  P DV I IRRANS
Sbjct: 1201 ESKREAGLGREGNSGNISRLNKTDREKNIDLGGQEDDFCLS-PVNLDPVDVGIGIRRANS 1260

Query: 1261 EGEYPIMADLSDTLDAVWKGVIQPNNGTFEESSNSGTNSAVLEPLSGQSDMEKCNATLVG 1320
             GEYPIM  +  TLDAVWKGVI PN+  F++S  S T SA+LEPL+GQSDMEKC A    
Sbjct: 1261 VGEYPIMT-VPGTLDAVWKGVIHPNSLIFDDSF-SRTTSAILEPLAGQSDMEKCTAN--R 1320

Query: 1321 VETAHLLHSAIVLTRDDSVETITSSTAMPSSAYYSKISLLNIPKPNISDYDHAFISSFRE 1380
            +ETAHLLHSA+VLTRDDSVETI +ST+MP SAY +KISLLNIPK +ISDY   FISS+RE
Sbjct: 1321 IETAHLLHSALVLTRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRE 1380

Query: 1381 LEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSL 1440
            LEKQ HLRLLMHVGSSDTVVPVYDEEPTSIIAYS+LMPEY  QMSEP K +E  DT+RSL
Sbjct: 1381 LEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSL 1440

Query: 1441 PIIGSAKLSSLKTSDEEGSG----------TILSMPKTER-QFEDRVSIAQNKHTRVSFV 1500
            P+IGS K++S+KT DEEGS           TILSMP+TE  QFED V ++++ HTRV F+
Sbjct: 1441 PVIGSVKMNSIKTCDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFI 1500

Query: 1501 NETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLD 1560
            NETPLGPVKYTIT +FAKRFEALRK+CCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLD
Sbjct: 1501 NETPLGPVKYTITCYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLD 1560

Query: 1561 ERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLKGGKECK 1620
            ERFIIKQVTKIEL+SFIQF+ AYF+YLS+A  T CPTCLAKVVG+YQVSSK+LKGGKECK
Sbjct: 1561 ERFIIKQVTKIELESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECK 1620

Query: 1621 IDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPIFLGSKA 1680
            +D+LVMENLLFRHNVT+LYDLKGSSRSRYN DTSGKN VLLDQNLIESMPTSPIFLGSKA
Sbjct: 1621 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKA 1680

Query: 1681 KRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLETWLKNS 1740
            KRLLERAVWNDTSFLASI VMDYSLLVGLDEEKQELVVGIID+MRQYTWDKHLETW+KNS
Sbjct: 1681 KRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNS 1740

Query: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDSWPSSTVSPSESHPDLCDDGSEPE 1749
            GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPD WPSSTVSPSESHPDLCD+ SEPE
Sbjct: 1741 GILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPE 1794

BLAST of Sed0006163 vs. ExPASy TrEMBL
Match: A0A6J1EUT4 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438136 PE=4 SV=1)

HSP 1 Score: 2696.8 bits (6989), Expect = 0.0e+00
Identity = 1390/1785 (77.87%), Postives = 1516/1785 (84.93%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            MGHPENKLS+LVGL KSW+  WRS+ ENV+RDFWMPD+SCRVCY+CDSQFTFI RRHHCR
Sbjct: 1    MGHPENKLSDLVGLVKSWM-PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFCSKCT+N I APSTD TN  EDSEKIRVCNYCFKQ G+EP  +QDGDKAT SA+
Sbjct: 61   RCGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAV 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SLSSSS+S+ SS+SDYTC SA+SN+NSTPCSN  QYYDPY S  ITDEQD+LR+ KSINR
Sbjct: 121  SLSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINR 180

Query: 181  TTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNLDG 240
            TT    S+S YY  G+YR DDE+DDYYYG YRSDSE++HISQSD+YYC VNADEGL LDG
Sbjct: 181  TTSLLTSSSSYY--GHYRSDDEEDDYYYGMYRSDSESRHISQSDNYYCDVNADEGLKLDG 240

Query: 241  ENICTKGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKNNEPMDFQNNGVL 300
            E+I  KGS V+FD  CPR +EE SSEDSDENDKGSECDFP Y MQN NNE MDFQNNG+L
Sbjct: 241  EDISKKGSPVMFDNQCPRDSEE-SSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGIL 300

Query: 301  WLPPEPEDEEDDKLLFFDDDNDSRSPGKW--APSFSLGSGEYPMKGRSSEEYRKTAKYVV 360
            WLPPEPEDEED+K L  DDD+DS +PG+W   PS SL SGE P+KGRSSEE+RKTAK VV
Sbjct: 301  WLPPEPEDEEDEKTLLLDDDDDSCAPGEWGYVPS-SLDSGECPVKGRSSEEHRKTAKNVV 360

Query: 361  EGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPDMSKSGPMDPSGYVK 420
            EGHFRALV QLLEAE+LPIGD P  DGWLDIITYLSWEAA VLKPD SKSG MDP GYVK
Sbjct: 361  EGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVK 420

Query: 421  VKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALEYQRVINHVSSLDTL 480
            VK VAGGKRSESIIVKGIICKKNVAH+RMTREISK   FVLGGALEYQRV NH+SSLDTL
Sbjct: 421  VKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTL 480

Query: 481  MQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVLNIKRSLLERIASCT 540
            +QQELDHLKMAVAKID+HHPN+LLVE+SVSRHAQEYL++KNIT+VLNIKRSLLERIA CT
Sbjct: 481  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 540

Query: 541  GASIIPTVDHVASLKLGYCDAFRVETF-EVHDTGDGQASKTSAKTLMFFEGCPKPLCCTV 600
            GASIIPTVDHVASLKLGYCD FRVETF E H +GDGQA K + KTLMFFEGCPKPLCCT+
Sbjct: 541  GASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTI 600

Query: 601  LLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPSKSPLTVILPHNPSN 660
            LLRGA  DELKKVKHVVQYATFAAYHLALETCFLADEGASVPELP  SP+TV LPHNPSN
Sbjct: 601  LLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN 660

Query: 661  TDRSISSIPGFTCPSPELNT------EIGQSRKDAVPNKDSSIIRNNVANLDSLEPEFIE 720
             DRSIS+IPGFTCPSP+ ++      EIGQ  KDAVPN+DSSII+ N+ +LD+       
Sbjct: 661  IDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIIKANI-SLDN------- 720

Query: 721  ANRNLERQEYSIGTMTIKEAVGADDDTISSSGKDEASSQRCNPISISIDSLDGSTQASND 780
                                   DDDT+  SGK E SSQ  N I IS ++LDG+TQASN 
Sbjct: 721  ----------------------KDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNG 780

Query: 781  STSQRPESPKANPVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYG 840
            STSQRPES   N VTTKSS+EDFPLSPSDQQNILVSLSTRCVWKGT CDRAHLLRIKYYG
Sbjct: 781  STSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYG 840

Query: 841  SFDEPLGRFLRDNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLTLFY-REPEG 900
            SFDEPLGRFLRD LFDQ+YRC SC MPSEAHV+CYTHPQGSLTISAKKL   F   EPEG
Sbjct: 841  SFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEG 900

Query: 901  KIWMWHRCLKCPRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLH 960
            KIWMWHRCLKCPRTNGFPPATRR+I+                FSNNAAASRVAGCGHFLH
Sbjct: 901  KIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLH 960

Query: 961  RDCLRFYGFGRTVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAE 1020
            RDCLRFYGFGRTVACF+Y+AINVQSV IPP ++EF+H NLEWINKEANEVHNRA+TLFAE
Sbjct: 961  RDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAE 1020

Query: 1021 VYKALPLISNSESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNE 1080
            V KALPLI   E G+EQEDFGVNEL+HY+QELEQII MEKEDFEVHFNEVL         
Sbjct: 1021 VCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVL--------- 1080

Query: 1081 VPSGEVAIGQPRVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDE 1140
              SGE  +GQP VDIFEINKLHMHILFLSY WVQRLSYA  LR+KNSP SSSND+  LDE
Sbjct: 1081 --SGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDE 1140

Query: 1141 RELDSLEQLVKFTLESRRDEFFDGFDNDSLPLESKHEIDLGNGGYSGQISQSNEMEREKN 1200
            R ++S+E+ V+FTL   +D+FF G DNDSLPLESKHEIDLG GG SGQISQ NE EREK+
Sbjct: 1141 RGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKD 1200

Query: 1201 FDLGGLEDDLCLSPPVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQPNNGT 1260
             DLG LEDDLCLSP    +P+DVEIS+RRANSEG+YPIMADLSDTLDAVWKGV+ PN+ T
Sbjct: 1201 IDLGVLEDDLCLSP---VMPQDVEISMRRANSEGDYPIMADLSDTLDAVWKGVMHPNSAT 1260

Query: 1261 FEESSNSGTNSAVLEPLSGQSDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAM 1320
              +SS S TNSA+LEPL GQSDMEKCNA LVG ETA LLHSA+VLT  DSVET TS  +M
Sbjct: 1261 -SDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSM 1320

Query: 1321 PSSAYYSKISLLNIPKPNISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPT 1380
            PSSAY +KISLLNIPKP+I +YD AFISSFRELEKQ +LRLLMHVGS+DTVVPVYDEEPT
Sbjct: 1321 PSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPT 1380

Query: 1381 SIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG-------- 1440
            SIIAYSLL PEYN QMSEPE+ LEP +T+RSLPIIGSAK++S+KT DEE +         
Sbjct: 1381 SIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSK 1440

Query: 1441 --TILSMPKTER-QFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1500
              TILSMP+ E  QFED +   Q+ HTRV FVNETPLGPVKYTIT +FAKRFEALRK+CC
Sbjct: 1441 EETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCC 1500

Query: 1501 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1560
            PSELDYVRS+SRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIEL+SF QFA AYF+YLS
Sbjct: 1501 PSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLS 1560

Query: 1561 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1620
            EA RT CPTCLAKVVG+YQVSSK+ KGG+ECK+DILVMENLLFRHNVT+LYDLKGSSRSR
Sbjct: 1561 EAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSR 1620

Query: 1621 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1680
            YNSDTSGKN VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASI VMDYSLLVG
Sbjct: 1621 YNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVG 1680

Query: 1681 LDEEKQELVVGIIDYMRQYTWDKHLETWLKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1740
            LDEEKQELVVGIID+MRQYTWDKHLETW+KNSGILGGPKNAAPTVISPHQYKKRFRKAMT
Sbjct: 1681 LDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1735

Query: 1741 TYFLMVPDSWPSSTVSPSESHPDLCDDGSEPENSWDGKHDSEQSD 1748
            TYFLMVPD WPSSTVSPSESHPDLC++ SEPE+SWDGK    QSD
Sbjct: 1741 TYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1735

BLAST of Sed0006163 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 865/1807 (47.87%), Postives = 1133/1807 (62.70%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            M   ++K    V + KSWI   +S S N++RDFWMPD+SC VCYECD+QFT   RRHHCR
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFC+KC +N I +PS ++ +  E+ E+IRVCNYC+KQ  +      +G    +S I
Sbjct: 61   LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SL  SSS    S +  T  S+   I+ST   +     +P  S  ++   DS +S +  N 
Sbjct: 121  SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180

Query: 181  TTPRTASTSGYYYD------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYC 240
             + R++   G+  D            G   G+ +DDD Y   +      +  +Q +DYY 
Sbjct: 181  KSRRSSDHYGHVLDSSDNQVEFFVNSGRSDGEADDDDDYQSDF-----AQSYAQGNDYYG 240

Query: 241  AVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECDF 300
            A+N DE  ++ G     ++  K    I   P  +  + L++E  D     EN      + 
Sbjct: 241  AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  PAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFSL 360
                 ++   E +DF+++G+LWLPPEPE+EED++  +  DDD D    G W    PS S 
Sbjct: 301  SPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSF 360

Query: 361  GSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSW 420
               ++  K +SS       K VVEGHFRALV QLLE ++LP+ +    +GWLDIIT LSW
Sbjct: 361  NEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSW 420

Query: 421  EAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSR 480
            EAAT+LKPD SKSG MDP GYVKVKC+  G+RSES++VKG++CKKNVAH+RMT +I K R
Sbjct: 421  EAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPR 480

Query: 481  IFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYL 540
            + +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEYL
Sbjct: 481  LLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYL 540

Query: 541  VAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQA 600
            +AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F        Q 
Sbjct: 541  LAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQV 600

Query: 601  SKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEG 660
            +K  AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY  FAAYHLALET FLADEG
Sbjct: 601  AKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEG 660

Query: 661  ASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVPN 720
            AS+ ELP ++P+TV LP  PS  +RSIS+IPGFT       P+ EL  E  ++  D   N
Sbjct: 661  ASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGN 720

Query: 721  KDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGKD 780
              SS    +  +   D ++P         ER  +++ T+  K  E + + DD +  + + 
Sbjct: 721  FTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLES 780

Query: 781  EASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPLS 840
               S      S+  D    L G+T+   D       +   N         +SS+ DF  S
Sbjct: 781  RQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPS 840

Query: 841  PSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSM 900
             SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ   C SC+M
Sbjct: 841  ASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTM 900

Query: 901  PSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII- 960
            P+EAH+HCYTH QGSLTIS KKL  L   + EGKIWMWHRCLKCPR NGFPPATRRI++ 
Sbjct: 901  PAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMS 960

Query: 961  ---------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVL 1020
                           FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V 
Sbjct: 961  DAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVT 1020

Query: 1021 IPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELSH 1080
            +PP  L F++ N EW+ KE+ EV  +A+ LF EV +AL  IS    G   +    N++  
Sbjct: 1021 LPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKL 1080

Query: 1081 YVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHILF 1140
             ++EL  ++   K++++    ++L+             V  GQP +DI  INKL   I+F
Sbjct: 1081 SLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLIIF 1140

Query: 1141 LSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFDN 1200
             SY W + L+ AA + + N  ++  N  P +  R + SLE+L    ++S          N
Sbjct: 1141 DSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AICN 1200

Query: 1201 DSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDVE 1260
            DSL  ++ +E  L  G      S    +      D+G      C     P        VE
Sbjct: 1201 DSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFVE 1260

Query: 1261 IS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQS 1320
             S  ++ A++E ++    DLSDTLDA W G      NG F   S + + +    P     
Sbjct: 1261 SSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP----- 1320

Query: 1321 DMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKPN 1380
                 +  L+G E      S +      + +  T+   +PS ++Y   +K   LN  K  
Sbjct: 1321 -----DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHI 1380

Query: 1381 ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMSE 1440
            +++    ++SS+RELE +   RLL+ +G +D V+PVYD+EPTSIIAY+L   EY  QMS 
Sbjct: 1381 MAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSG 1440

Query: 1441 PEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNKH 1500
             +K+ +  D+  S  +  S  L SL +  +       S+   + Q    +  +   ++ H
Sbjct: 1441 SDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLH 1500

Query: 1501 TRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNVF 1560
             R+SF +E P G VKY++T ++AK FEALR  CCPSE D++RSL RC++WGAQGGKSNVF
Sbjct: 1501 ARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVF 1560

Query: 1561 FAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYLK 1620
            FAK+LD+RFIIKQVTK EL+SFI+F  AYF YL+E+  T  PT LAK++GIYQVSSK+LK
Sbjct: 1561 FAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLK 1620

Query: 1621 GGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSPI 1680
            GGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSPI
Sbjct: 1621 GGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPI 1680

Query: 1681 FLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHLE 1736
            F+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHLE
Sbjct: 1681 FVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLE 1740

BLAST of Sed0006163 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1133/1808 (62.67%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            M   ++K    V + KSWI   +S S N++RDFWMPD+SC VCYECD+QFT   RRHHCR
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFC+KC +N I +PS ++ +  E+ E+IRVCNYC+KQ  +      +G    +S I
Sbjct: 61   LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SL  SSS    S +  T  S+   I+ST   +     +P  S  ++   DS +S +  N 
Sbjct: 121  SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180

Query: 181  TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
             + R++   G+  D             G   G+ +DDD Y   +      +  +Q +DYY
Sbjct: 181  KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240

Query: 241  CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
             A+N DE  ++ G     ++  K    I   P  +  + L++E  D     EN      +
Sbjct: 241  GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300

Query: 301  FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
                  ++   E +DF+++G+LWLPPEPE+EED++  +  DDD D    G W    PS S
Sbjct: 301  GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360

Query: 361  LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
                ++  K +SS       K VVEGHFRALV QLLE ++LP+ +    +GWLDIIT LS
Sbjct: 361  FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
            WEAAT+LKPD SKSG MDP GYVKVKC+  G+RSES++VKG++CKKNVAH+RMT +I K 
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
            R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
            L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F        Q
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600

Query: 601  ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
             +K  AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
            GAS+ ELP ++P+TV LP  PS  +RSIS+IPGFT       P+ EL  E  ++  D   
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
            N  SS    +  +   D ++P         ER  +++ T+  K  E + + DD +  + +
Sbjct: 721  NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780

Query: 781  DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
                S      S+  D    L G+T+   D       +   N         +SS+ DF  
Sbjct: 781  SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840

Query: 841  SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
            S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ   C SC+
Sbjct: 841  SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
            MP+EAH+HCYTH QGSLTIS KKL  L   + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901  MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960

Query: 961  ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
                            FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961  SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020

Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
             +PP  L F++ N EW+ KE+ EV  +A+ LF EV +AL  IS    G   +    N++ 
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080

Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
              ++EL  ++   K++++    ++L+             V  GQP +DI  INKL   I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKDSLQQMLN------------VVKDGQPTIDILLINKLRRLII 1140

Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
            F SY W + L+ AA + + N  ++  N  P +  R + SLE+L    ++S          
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200

Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
            NDSL  ++ +E  L  G      S    +      D+G      C     P        V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260

Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
            E S  ++ A++E ++    DLSDTLDA W G      NG F   S + + +    P    
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320

Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
                  +  L+G E      S +      + +  T+   +PS ++Y   +K   LN  K 
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380

Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
             +++    ++SS+RELE +   RLL+ +G +D V+PVYD+EPTSIIAY+L   EY  QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440

Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
              +K+ +  D+  S  +  S  L SL +  +       S+   + Q    +  +   ++ 
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500

Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
            H R+SF +E P G VKY++T ++AK FEALR  CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560

Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
            FFAK+LD+RFIIKQVTK EL+SFI+F  AYF YL+E+  T  PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620

Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
            KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680

Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
            IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740

BLAST of Sed0006163 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 1476.5 bits (3821), Expect = 0.0e+00
Identity = 865/1808 (47.84%), Postives = 1130/1808 (62.50%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCR 60
            M   ++K    V + KSWI   +S S N++RDFWMPD+SC VCYECD+QFT   RRHHCR
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPR-KSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60

Query: 61   FCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAI 120
             CGRVFC+KC +N I +PS ++ +  E+ E+IRVCNYC+KQ  +      +G    +S I
Sbjct: 61   LCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNG----ASII 120

Query: 121  SLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLRSGKSINR 180
            SL  SSS    S +  T  S+   I+ST   +     +P  S  ++   DS +S +  N 
Sbjct: 121  SLHFSSSPSARSVASTTSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQ-NA 180

Query: 181  TTPRTASTSGYYYD-------------GYYRGDDEDDDYYYGTYRSDSETKHISQSDDYY 240
             + R++   G+  D             G   G+ +DDD Y   +      +  +Q +DYY
Sbjct: 181  KSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDF-----AQSYAQGNDYY 240

Query: 241  CAVNADEGLNLDG----ENICTKGSAVIFDTPCPRSNEELSSEDSD-----ENDKGSECD 300
             A+N DE  ++ G     ++  K    I   P  +  + L++E  D     EN      +
Sbjct: 241  GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300

Query: 301  FPAYGMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK-LLFFDDDNDSRSPGKWA---PSFS 360
                  ++   E +DF+++G+LWLPPEPE+EED++  +  DDD D    G W    PS S
Sbjct: 301  GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNS 360

Query: 361  LGSGEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLS 420
                ++  K +SS       K VVEGHFRALV QLLE ++LP+ +    +GWLDIIT LS
Sbjct: 361  FNEKDFHSKDKSS----GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKS 480
            WEAAT+LKPD SKSG MDP GYVKVKC+  G+RSES++VKG++CKKNVAH+RMT +I K 
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RIFVLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEY 540
            R+ +LGGALEYQR+ N +SS DTL+QQE+DHLKMAVAKID+H+P+ILLVE+SVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LVAKNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQ 600
            L+AK+I++VLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE F        Q
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQ 600

Query: 601  ASKTSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADE 660
             +K  AKTLMFF+GCPKPL CT+LL+GA +DELKKVKHV+QY  FAAYHLALET FLADE
Sbjct: 601  VAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADE 660

Query: 661  GASVPELPSKSPLTVILPHNPSNTDRSISSIPGFT------CPSPELNTEIGQSRKDAVP 720
            GAS+ ELP ++P+TV LP  PS  +RSIS+IPGFT       P+ EL  E  ++  D   
Sbjct: 661  GASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTG 720

Query: 721  NKDSS--IIRNNVANLDSLEPEFIEANRNLERQEYSIGTMTIK--EAVGADDDTISSSGK 780
            N  SS    +  +   D ++P         ER  +++ T+  K  E + + DD +  + +
Sbjct: 721  NFTSSKTHFQGKLDGNDRIDPS--------ERLLHNLDTVYCKPPETITSKDDGLVPTLE 780

Query: 781  DEASSQRCNPISISIDS---LDGSTQASNDSTSQRPESPKAN-----PVTTKSSREDFPL 840
                S      S+  D    L G+T+   D       +   N         +SS+ DF  
Sbjct: 781  SRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHP 840

Query: 841  SPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCS 900
            S SD Q+ILVSLSTRCVWKG+ C+RAHLLRIKYYGSFD+PLGRFLRDNLFDQ   C SC+
Sbjct: 841  SASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCT 900

Query: 901  MPSEAHVHCYTHPQGSLTISAKKLLTLFYREPEGKIWMWHRCLKCPRTNGFPPATRRIII 960
            MP+EAH+HCYTH QGSLTIS KKL  L   + EGKIWMWHRCLKCPR NGFPPATRRI++
Sbjct: 901  MPAEAHIHCYTHRQGSLTISVKKLPELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVM 960

Query: 961  ----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSV 1020
                            FSN+AAASRVA CGH LHRDCLRFYGFGR VACF Y++IN+ +V
Sbjct: 961  SDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAV 1020

Query: 1021 LIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNELS 1080
             +PP  L F++ N EW+ KE+ EV  +A+ LF EV +AL  IS    G   +    N++ 
Sbjct: 1021 TLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIK 1080

Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
              ++EL  ++   K++++   N V                  GQP +DI  INKL   I+
Sbjct: 1081 LSLEELAGLLEQRKKEYKQMLNVVKD----------------GQPTIDILLINKLRRLII 1140

Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
            F SY W + L+ AA + + N  ++  N  P +  R + SLE+L    ++S          
Sbjct: 1141 FDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKVKSIPTHV--AIC 1200

Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLC---LSPPVNSLPRDV 1260
            NDSL  ++ +E  L  G      S    +      D+G      C     P        V
Sbjct: 1201 NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEGGKDNFV 1260

Query: 1261 EIS--IRRANSEGEYPIMADLSDTLDAVWKG-VIQPNNGTFEESSNSGTNSAVLEPLSGQ 1320
            E S  ++ A++E ++    DLSDTLDA W G      NG F   S + + +    P    
Sbjct: 1261 ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP---- 1320

Query: 1321 SDMEKCNATLVGVETAHLLHSAIVLTRDDSVETITSSTAMPSSAYY---SKISLLNIPKP 1380
                  +  L+G E      S +      + +  T+   +PS ++Y   +K   LN  K 
Sbjct: 1321 ------DLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKH 1380

Query: 1381 NISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQMS 1440
             +++    ++SS+RELE +   RLL+ +G +D V+PVYD+EPTSIIAY+L   EY  QMS
Sbjct: 1381 IMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMS 1440

Query: 1441 EPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSGTILSMPKTERQFEDRVSIA---QNK 1500
              +K+ +  D+  S  +  S  L SL +  +       S+   + Q    +  +   ++ 
Sbjct: 1441 GSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDL 1500

Query: 1501 HTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRCKRWGAQGGKSNV 1560
            H R+SF +E P G VKY++T ++AK FEALR  CCPSE D++RSL RC++WGAQGGKSNV
Sbjct: 1501 HARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNV 1560

Query: 1561 FFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAKVVGIYQVSSKYL 1620
            FFAK+LD+RFIIKQVTK EL+SFI+F  AYF YL+E+  T  PT LAK++GIYQVSSK+L
Sbjct: 1561 FFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHL 1620

Query: 1621 KGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLLDQNLIESMPTSP 1680
            KGGKE K+D+LVMENLLF+ N T+LYDLKGS+R+RYN DTSG NTVLLDQNL+E+MPTSP
Sbjct: 1621 KGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSP 1680

Query: 1681 IFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGIIDYMRQYTWDKHL 1736
            IF+GSKAKRLLERAVWNDTSFLASIHVMDYSLLVG+DEE+ ELV+GIID+MRQYTWDKHL
Sbjct: 1681 IFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHL 1740

BLAST of Sed0006163 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 882/1830 (48.20%), Postives = 1150/1830 (62.84%), Query Frame = 0

Query: 9    SELVGLAKSWISSWRSNSENVARDFWMPDRSCRVCYECDSQFTFIKRRHHCRFCGRVFCS 68
            SE+VGL KSW+  WRS    V+RDFWMPD+SCRVCYECD QFT I RRHHCR CGRVFC 
Sbjct: 12   SEIVGLIKSWL-PWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCG 71

Query: 69   KCTSNFIRAPSTDSTNCMEDSEKIRVCNYCFKQLGREPFALQDGDKATSSAISLSSSSS- 128
            KCT+N I    +D     ED E+IRVCNYCF+Q     +   DG    S+   LS+S S 
Sbjct: 72   KCTANSIPFAPSDLRTPREDWERIRVCNYCFRQ-----WEQGDGGPHVSNITELSTSPSE 131

Query: 129  -SMDSSKSDYTCQSALSNINSTP-----CSNSHQYYDPYCSFPITDEQDSLRSGKSINRT 188
             S+ SSK+  T  S+   + S P         H   D       + E    + GK  +R 
Sbjct: 132  TSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRR 191

Query: 189  TPRTAS---TSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVNADEGLNL 248
            +   A+       +     R DDE D+  YG Y++D ET H  ++++YY  +  + G+ +
Sbjct: 192  SSFIATDVEDPSRFALNSIRSDDEYDE--YGAYQTDIETSHSPRANEYYGPMEYN-GMGI 251

Query: 249  D--------GENICTK---GSAVIFDTPCPRSNEELSSED---SDENDKGSECD--FPAY 308
            D        GE    K   GS +I    C  S     SE      E+D   EC+   PA 
Sbjct: 252  DDVPCKHLGGETADQKSLSGSPLIHQ--CLESLIREGSEQFQKKSEHDGRDECEASSPAD 311

Query: 309  GMQNKNNEPMDFQNNGVLWLPPEPEDEEDDK--LLFFDDDNDSRSPGKWA---PSFSLGS 368
               ++  EP+DF+NNG+LW+PPEPE+EED++   LF ++DN+  + G+W    PS S GS
Sbjct: 312  ISDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGS 371

Query: 369  GEYPMKGRSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEA 428
            GEY  + R++EE++K  K VV+GHFRAL+ QLL+ E++ + D    + WL+IIT LSWEA
Sbjct: 372  GEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEA 431

Query: 429  ATVLKPDMSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIF 488
            A +LKPDMSKSG MDP GYVKVKC+A G R +S++VKG++CKKNV ++RM+ +I K+R+ 
Sbjct: 432  ANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLL 491

Query: 489  VLGGALEYQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVA 548
            +LGG LEYQRV N +SS DTL+QQE DHLKMAVAKI A  PNILLVE+SVSR AQEYL+A
Sbjct: 492  ILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLA 551

Query: 549  KNITVVLNIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVETFEVHDTGDGQASK 608
            K+I++VLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ +       GQ  K
Sbjct: 552  KDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGK 611

Query: 609  TSAKTLMFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
               KTLM+FE CPKPL  T+LLRGA++DELKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 612  KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671

Query: 669  VPELPSKSPLTVILPHNPSNTDRSISSIPGFTC----PSPELNTEIGQSRKDAVPNKDSS 728
             PELP  SP+TV LP   ++ +RSIS++PGFT      SP + +     R ++VP  +  
Sbjct: 672  -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELL 731

Query: 729  IIRNNVANLDSLEP-------EFIEANRNLERQEYSIGTMTIKEAV--GADDDTISSSGK 788
                N++    + P       +  E N +     ++I ++ + + V    + D    S  
Sbjct: 732  STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNI-SLNLPDRVIESRNSDLSGRSVP 791

Query: 789  DEASSQRCNPISISIDSLDGSTQAS-------------------NDSTSQRPESPKAN-- 848
             +  + + NPI ++ ++ + S   S                    D+ S+   + + N  
Sbjct: 792  VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 851

Query: 849  -PVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLR 908
             P  T+S +E+FP SPSD Q+ILVSLS+R VWKGT C+R+HL RIKYYGSFD+PLGRFLR
Sbjct: 852  KPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLR 911

Query: 909  DNLFDQSYRCHSCSMPSEAHVHCYTHPQGSLTISAKKLLT-LFYREPEGKIWMWHRCLKC 968
            D+LFDQSYRC SC MPSEAHVHCYTH QGSLTIS KKL   L   E EGKIWMWHRCL+C
Sbjct: 912  DHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRC 971

Query: 969  PRTNGFPPATRRIII----------------FSNNAAASRVAGCGHFLHRDCLRFYGFGR 1028
            PR NGFPPAT R+++                FSN+AAASRVA CGH LHRDCLRFYGFG 
Sbjct: 972  PRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGN 1031

Query: 1029 TVACFYYSAINVQSVLIPPPILEFHH-NLEWINKEANEVHNRAKTLFAEVYKALPLISNS 1088
             VACF Y+ I+V SV +PP IL F++ N +WI +E +EV  RA+ LF+EV  A+  I  +
Sbjct: 1032 MVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI--A 1091

Query: 1089 ESGTEQEDFGVNELSHYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQP 1148
            E G  +           + ELE+++  EK +FE +  ++L  E+   NE        GQP
Sbjct: 1092 EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREV---NE--------GQP 1151

Query: 1149 RVDIFEINKLHMHILFLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVK 1208
             VDI E+ ++H  +LF SY W  RL  A+ L K  + D +        +RE +    L K
Sbjct: 1152 LVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDT--------KREENEKPPLAK 1211

Query: 1209 FTLESRRDEFFDGFDNDSLPLESKHEIDLG-NGGYSGQISQSNEMERE--KNFDLGGLED 1268
                    +     +  +  L +  E++L  +G  +G     N +++E   N DL   +D
Sbjct: 1212 -------SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKD 1271

Query: 1269 DLC-LSP----PVNSLPRDVEISIRRANSEGEYPIMADLSDTLDAVWKGVIQ-----PNN 1328
            D   +SP    P  S P + ++ +RR  S+G+  +M +LS TLDA W G  Q     P N
Sbjct: 1272 DGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTN 1331

Query: 1329 GTFEESSNSGTNSAVLEPLSG---QSDM-EKCNATLVGVETAHLLHSAIVLTRDDSVETI 1388
                   ++ +NS+   P+S      D+ E+ N   V    +  L S      +DSV   
Sbjct: 1332 NKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSV--- 1391

Query: 1389 TSSTAMPSSAYYSKIS---LLNIPK-PNISDYDHAFISSFRELEKQEHLRLLMHVGSSDT 1448
             S  ++P   +Y  I+   LL+  K     ++   +ISSFRE E Q   RLL+ VG +D 
Sbjct: 1392 -SWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDI 1451

Query: 1449 VVPVYDEEPTSIIAYSLLMPEYNVQMSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEG 1508
            VVPVYD+EPTS+IAY+L+ PEY  Q S   ++L    +  ++P      +     S+   
Sbjct: 1452 VVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNGSV 1511

Query: 1509 SGTILSMPKT-ERQFEDRVSIAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCC 1568
              +ILS+  +      D +S  +  H RVS+  +  LG VKYT+T ++AKRFEALR  C 
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571

Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLS 1628
            PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK EL+SFI+FA AYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631

Query: 1629 EATRTTCPTCLAKVVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSR 1688
            E+  T  PTCLAK++GIYQV++K LK GKE K+D+L+MENLLF   V +LYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691

Query: 1689 YNSDTSGKNTVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVG 1733
            YN D+SG N VLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLA   VMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751

BLAST of Sed0006163 vs. TAIR 10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )

HSP 1 Score: 984.6 bits (2544), Expect = 1.1e-286
Identity = 651/1799 (36.19%), Postives = 958/1799 (53.25%), Query Frame = 0

Query: 1    MGHPENKLSELVGLAKSWISSWRSNS----ENVARDFWMPDRSCRVCYECDSQFTFIKRR 60
            MG P+  L +L+   +SWI+S  S+S     +  +DF +     ++C++C    T +++ 
Sbjct: 1    MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60

Query: 61   HHCRFCGRVFCSKCTSNFIRAPSTDSTNCMEDSEKIRVCNYC---FKQLGREPFALQDGD 120
            + C  CG  +C  C+                +  K+++C  C    ++L  + +      
Sbjct: 61   YCCLSCGSCWCKSCSDT--------------EESKMKLCRECDAEVRELRVKSYDKVHPR 120

Query: 121  KATSSAISLSSSSSSMDSSKSDYTCQSALSNINSTPCSNSHQYYDPYCSFPITDEQDSLR 180
             +     SL++ S S+ SS     C+    N+ S  C  S             +E+++  
Sbjct: 121  DSPDPPSSLATESESLASSLEIRDCR----NMASIRCYPSR-----------GEEEEARY 180

Query: 181  SGKSINRTTPRTASTSGYYYDGYYRGDDEDDDYYYGTYRSDSETKHISQSDDYYCAVN-- 240
             GK +   +      S     G      E     +    S   + H S   + +  +   
Sbjct: 181  CGKQLLSPSSDNYQDSSDIESGSVSARHE----LFSCKSSAGSSPHDSPLRNNFSPLGRF 240

Query: 241  ADEGLNLDGENICT---KGSAVIFDTPCPRSNEELSSEDSDENDKGSECDFPAYGMQNKN 300
                 +L    +C+       ++ D         L  ED +E              ++K 
Sbjct: 241  VQHAKDLRSPTVCSFDNHQEQLLADNLVKPGQGVLEQEDHEEE-------------EDKL 300

Query: 301  NEPMDFQNNGVLWLPPEPEDEEDD-KLLFFDDDNDSRSPGKWAPSFSLG---SGEYPMKG 360
             +P+DF+NNG +W PP PEDE DD +  +F  D++    G  A  FSL    S   P K 
Sbjct: 301  QQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIPTKE 360

Query: 361  RSSEEYRKTAKYVVEGHFRALVGQLLEAEDLPIGDGPSGDGWLDIITYLSWEAATVLKPD 420
            +  E   +  + VV  HFRALV +LL  E+L   D  S   WLDI+T L+W+AA  +KPD
Sbjct: 361  KLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPD 420

Query: 421  MSKSGPMDPSGYVKVKCVAGGKRSESIIVKGIICKKNVAHKRMTREISKSRIFVLGGALE 480
                G MDP  YVK+KCVA G ++ESI+++GI+C KN+ HKRM  +    R+ +L G+LE
Sbjct: 421  TRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLE 480

Query: 481  YQRVINHVSSLDTLMQQELDHLKMAVAKIDAHHPNILLVERSVSRHAQEYLVAKNITVVL 540
            YQRV   ++S +TL+QQE +H+K  +AKI++  PN+LLVE+S S +AQ+YL+ K I++VL
Sbjct: 481  YQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVL 540

Query: 541  NIKRSLLERIASCTGASIIPTVDHVASLKLGYCDAFRVE-TFEVHDTGDGQASKTSAKTL 600
            N+KRSLL+RIA CTGA + P++D +++ +LG+C+ FR E   E H+ G+ Q+++  ++TL
Sbjct: 541  NVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKPSRTL 600

Query: 601  MFFEGCPKPLCCTVLLRGASKDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPS 660
            M+FEGCP+ L CTV+LRG+ ++ELKKVKHV+QYA FAAYHL+LET FLADEGAS+P++  
Sbjct: 601  MYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRL 660

Query: 661  KSPLTV--------------ILPHNPSNTD-----RSISSIPGFTCPSPELNTEIGQSR- 720
            K P  V              ++  +P+ TD      + +     T P PE   E+ +S  
Sbjct: 661  KQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPE--HEVCESLC 720

Query: 721  KDAVPNK---DSSIIRNNVANLDSLEPEFIEANRNLERQEYSIGTMT-IKEAVGADDDTI 780
            +D  P +    SS +    +  D+L  +F     NL  + YS   +  + E        I
Sbjct: 721  EDFDPTQIFPPSSEVETEQS--DTLNGDFA---NNLVTRSYSSNQLNDLHEPTLCLSSEI 780

Query: 781  SSSGKDEASSQRCNPISISIDSLDGSTQASNDSTSQRPESPKANPVTTKSSREDFPLSPS 840
              +   + S +  N          G  +  N   + + + P+           ++  +  
Sbjct: 781  PETPTQQPSGEEDN----------GRGEEENQLVNPQ-DLPQHESFYEDDVSSEYFSAAD 840

Query: 841  DQQNILVSLSTRCVWKGTTCDRAHLLRIKYYGSFDEPLGRFLRDNLFDQSYRCHSCSMPS 900
              Q+ILVS S+RCV K + C+R+ LLRIK+YGSFD+PLGR+L+D+LFD++  C SC    
Sbjct: 841  SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900

Query: 901  EAHVHCYTHPQGSLTISAKKLLTL-FYREPEGKIWMWHRCLKCPRTNGFPPATRRIII-- 960
            +AHV CY+H  G+LTI+ ++L ++    E +GKIWMWHRCL+C   +G PPATRR+++  
Sbjct: 901  DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960

Query: 961  --------------FSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFYYSAINVQSVLI 1020
                          FSN+A A+RVA CGH L RDCLRFYGFG  VA F YS IN+ +VL+
Sbjct: 961  AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020

Query: 1021 PPPILEF--HHNLEWINKEANEVHNRAKTLFAEVYKALPLISNSESGTEQEDFGVNEL-S 1080
            PP +LEF  H   EWI  EA E+  + +T++ E+   L  +    S  E E     +L S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080

Query: 1081 HYVQELEQIINMEKEDFEVHFNEVLSGEIPMKNEVPSGEVAIGQPRVDIFEINKLHMHIL 1140
              +  ++Q++  EK++++     +    + +            Q  +DI E+N+L   ++
Sbjct: 1081 RIIGLIDQLVK-EKDEYDDALQPIFEENLQI------------QGSLDILELNRLRRALM 1140

Query: 1141 FLSYTWVQRLSYAAALRKKNSPDSSSNDIPMLDERELDSLEQLVKFTLESRRDEFFDGFD 1200
              ++ W  +L    +  KK S   + +D                      R  E  D   
Sbjct: 1141 IGAHAWDHQLYLLNSQLKKASVFKTGDD-------------------NAPRNPEMHDPPK 1200

Query: 1201 NDSLPLESKHEIDLGNGGYSGQISQSNEMEREKNFDLGGLEDDLCLSPPVNSLPRDVEIS 1260
             D    E   E D           + +  + E N D              N  P ++   
Sbjct: 1201 IDRRMQEGSDERD-----------EQSHTDSEANGD--------------NKDPENIP-- 1260

Query: 1261 IRRANSEGEYPIMADLSDTLDAVWKGVIQ--PNNGTFEESSNSGTNSAVLEPLSGQSDME 1320
                 S G       LS+ +D+ W G  Q      T  E+      ++ L  L+    ++
Sbjct: 1261 -----SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQ 1320

Query: 1321 KCNATL-------VGVETAHLLHSAI-VLTRDDSVETITSSTAMPSSAYYSKISLLNIPK 1380
              ++ +        G+  + L  S +           +           YS++  L + K
Sbjct: 1321 SFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQK 1380

Query: 1381 PN-ISDYDHAFISSFRELEKQEHLRLLMHVGSSDTVVPVYDEEPTSIIAYSLLMPEYNVQ 1440
             + I      +ISS  ++     + L+   G +D VVPVYD++P S+++Y++   EY   
Sbjct: 1381 LDLIVGSAPTYISSASQMADGARM-LIPQRGLNDIVVPVYDDDPASVVSYAINSKEYK-- 1440

Query: 1441 MSEPEKALEPGDTSRSLPIIGSAKLSSLKTSDEEGSG----TILSMPKTERQF------- 1500
                E  +  G       +  S+  S+L   + E S       LSM     Q        
Sbjct: 1441 ----EWIVNKG-------LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQ 1500

Query: 1501 EDRVS--IAQNKHTRVSFVNETPLGPVKYTITGFFAKRFEALRKRCCPSELDYVRSLSRC 1560
            +DR S  +  +   R S  +    G VK+++T +FA +F+ LRK CCPSE+D+VRSLSRC
Sbjct: 1501 DDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRC 1560

Query: 1561 KRWGAQGGKSNVFFAKTLDERFIIKQVTKIELQSFIQFASAYFMYLSEATRTTCPTCLAK 1620
            +RW AQGGKSNV+FAK+LDERFIIKQV K EL SF  FA  YF YL E+  +  PTCLAK
Sbjct: 1561 QRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAK 1620

Query: 1621 VVGIYQVSSKYLKGGKECKIDILVMENLLFRHNVTQLYDLKGSSRSRYNSDTSGKNTVLL 1680
            ++GIYQVS K+ KGGKE K+D++VMENL +   ++++YDLKGS+RSRYN +TSG + VLL
Sbjct: 1621 ILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLL 1648

Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGLDEEKQELVVGII 1715
            D NL+E++ T PIFLGSKAKR LERA+WNDT+FLAS+ VMDYSLLVG DEE++ELV+GII
Sbjct: 1681 DMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGII 1648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877586.10.0e+0077.331-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Benincasa his... [more]
KAG7020853.10.0e+0077.811-phosphatidylinositol-3-phosphate 5-kinase FAB1B [Cucurbita argyrosperma subsp.... [more]
XP_023520487.10.0e+0077.921-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pep... [more]
KAG6588292.10.0e+0077.811-phosphatidylinositol-3-phosphate 5-kinase FAB1B, partial [Cucurbita argyrosper... [more]
XP_016899810.10.0e+0077.01PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1... [more]
Match NameE-valueIdentityDescription
Q0WUR50.0e+0047.841-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=370... [more]
Q9LUM00.0e+0048.201-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=370... [more]
Q9SSJ81.5e-28536.19Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thalia... [more]
Q9XID07.1e-17932.04Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thalia... [more]
Q9Z1T69.5e-9923.481-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve ... [more]
Match NameE-valueIdentityDescription
A0A1S4DVR60.0e+0077.011-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103487... [more]
A0A6J1EZX40.0e+0077.701-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KWW80.0e+0077.771-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0M3A50.0e+0076.461-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A6J1EUT40.0e+0077.871-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G33240.20.0e+0047.871-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT4G33240.10.0e+0047.841-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT4G33240.30.0e+0047.841-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT3G14270.10.0e+0048.20phosphatidylinositol-4-phosphate 5-kinase family protein [more]
AT1G71010.11.1e-28636.19FORMS APLOID AND BINUCLEATE CELLS 1C [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000306FYVE zinc fingerSMARTSM00064fyve_4coord: 29..104
e-value: 7.1E-18
score: 75.4
IPR000306FYVE zinc fingerPFAMPF01363FYVEcoord: 34..102
e-value: 5.1E-17
score: 61.8
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, coreSMARTSM00330PIPK_2coord: 1415..1706
e-value: 2.5E-116
score: 402.5
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePFAMPF01504PIP5Kcoord: 1481..1647
e-value: 5.1E-33
score: 114.5
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROSITEPS51455PIPKcoord: 1381..1705
score: 56.237225
NoneNo IPR availableGENE3D3.30.810.10coord: 1564..1706
e-value: 4.8E-42
score: 145.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 734..798
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1725..1748
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 743..798
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1713..1748
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..344
NoneNo IPR availablePANTHERPTHR457481-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATEDcoord: 1..1728
NoneNo IPR availablePANTHERPTHR45748:SF171-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE FAB1Bcoord: 1..1728
NoneNo IPR availableCDDcd03334Fab1_TCPcoord: 363..635
e-value: 3.42842E-120
score: 376.947
NoneNo IPR availableSUPERFAMILY56104SAICAR synthase-likecoord: 1409..1705
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 21..106
e-value: 1.2E-19
score: 71.9
IPR027484Phosphatidylinositol-4-phosphate 5-kinase, N-terminalGENE3D3.30.800.10Phosphatidylinositol Phosphate Kinase II Betacoord: 1396..1562
e-value: 8.8E-33
score: 115.6
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 389..624
e-value: 1.3E-32
score: 113.1
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 428..601
e-value: 7.4E-50
score: 171.2
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 429..601
IPR017455Zinc finger, FYVE-relatedPROSITEPS50178ZF_FYVEcoord: 37..103
score: 13.000669
IPR0447691-phosphatidylinositol-3-phosphate 5-kinase, PIPK catalytic domainCDDcd17300PIPKc_PIKfyvecoord: 1442..1705
e-value: 3.83605E-140
score: 431.935
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 32..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0006163.1Sed0006163.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010256 endomembrane system organization
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0009555 pollen development
biological_process GO:0007033 vacuole organization
biological_process GO:0046488 phosphatidylinositol metabolic process
cellular_component GO:0010008 endosome membrane
molecular_function GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016307 phosphatidylinositol phosphate kinase activity