Sed0005735 (gene) Chayote v1

BLAST of Sed0005735 vs. NCBI nr
Match: XP_038894856.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Benincasa hispida])

HSP 1 Score: 2269.2 bits (5879), Expect = 0.0e+00
Identity = 1145/1337 (85.64%), Postives = 1230/1337 (92.00%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MSKSCM  ASSSSPTQCPP VCENERLDLNSI+GLVVS+NQYI EFL N E RTA+KLRC
Sbjct: 1    MSKSCMLTASSSSPTQCPPSVCENERLDLNSIRGLVVSINQYIQEFLSNAEVRTAVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
            T+KLR QRHGF EFLEQSIISNLY GIE IEDA+Q STSEARA+ L TAEQMLQVPALLD
Sbjct: 61   TSKLRNQRHGFLEFLEQSIISNLYGGIEYIEDAVQTSTSEARATRLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            E GETSGM N YLVCCSYFYLS+VKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQ FC++
Sbjct: 121  EDGETSGMANRYLVCCSYFYLSLVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYN 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSF+TCSRQEN +SM S+S VEFGEGDYGES +RQVARKYKDWLMYYQVMSYGET QW
Sbjct: 181  LLLSFSTCSRQENCKSMGSNSFVEFGEGDYGESTIRQVARKYKDWLMYYQVMSYGETHQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGS S+LSSEDGSHSLHG+FSR E+S+ATD G   PTL HYD +PPLD   VFQDK +
Sbjct: 241  QQQGSRSMLSSEDGSHSLHGTFSRIEASEATDCGFPRPTLSHYDILPPLDYIDVFQDKRK 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
            ASQDF +CED  + PKKLG IPE+QFS+RG W DSSTKCIGDLLKDS PG+PTSLFSSMN
Sbjct: 301  ASQDFLQCEDTANSPKKLGFIPERQFSERGFWRDSSTKCIGDLLKDSQPGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            +SESDSDLEAG+N  +HSK++ RADMPE+ YQKLQYACS +D EQ  +SLSS S+S V+E
Sbjct: 361  NSESDSDLEAGMNDTNHSKRTARADMPENFYQKLQYACSNTDGEQSLISLSSASLSRVKE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
             Y ++NM KS SN+   +K  S++QKNLEPQ+FQN LEESE  ELS++PCKLQ+FDSA+P
Sbjct: 421  QYNKSNMMKSISNKFNGYKSRSLKQKNLEPQVFQNCLEESEPKELSVDPCKLQSFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            LS  QGSA QIS Q   KGQLYH+N+ RDSKSEILGLV KAISRLCFSEG G+Y++ECAV
Sbjct: 481  LSLGQGSACQISKQISVKGQLYHANSRRDSKSEILGLVEKAISRLCFSEGLGDYNDECAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALK NVHEAA LIYLISPSPREIKSLELLPV VEIICTSKCYN WS
Sbjct: 601  DIKKKGLQLCDLATALKHNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNAWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            PSL+LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGL SLGS
Sbjct: 661  PSLVLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLASLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECRGYISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRGYISKFISVAPFLCLLQSDKKEAVHIALQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEGKNDIIHILMLCVNHLE EYQL AANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV
Sbjct: 781  RIKNEGKNDIIHILMLCVNHLETEYQLWAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILST+GGTF+ TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTIGGTFSWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCSLMARNII IGE VFHALEKGLKS  KKVSRDCLT IAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSLMARNIIYIGESVFHALEKGLKSNIKKVSRDCLTAIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL GIE FLHPGVELEERLLACLCI+NYTSGKGMQKL+ FSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTRFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQHLGA+GQ+IFAITHGHGLKVIDASRT+KVLFKSKN KCIKVVQARVY GCTDSSI
Sbjct: 1141 KEQIQHLGAYGQMIFAITHGHGLKVIDASRTTKVLFKSKNLKCIKVVQARVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIKPPSKSW++MHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRH+KPE
Sbjct: 1201 QEFSVTNKWEQEIKPPSKSWMLMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHEKPE 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            + I TGKGDIVQAMS+VEDFVY+I KSS N+IQIWLRK QHKVGRV+AGS+ITCLLTAND
Sbjct: 1261 INIITGKGDIVQAMSVVEDFVYIICKSSVNSIQIWLRKAQHKVGRVTAGSKITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIPL
Sbjct: 1321 MVLCGTETGKIKGWIPL 1337

BLAST of Sed0005735 vs. NCBI nr
Match: XP_023526729.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo] >XP_023526731.1 putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo] >XP_023526732.1 putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2264.6 bits (5867), Expect = 0.0e+00
Identity = 1142/1337 (85.42%), Postives = 1220/1337 (91.25%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MS SCMA ASSSSPTQCPP VCEN RLDLNSI+GLVVS+NQYIHEFL N EARTA+KLRC
Sbjct: 1    MSISCMATASSSSPTQCPPGVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
             +KLR  +H +FEFLEQSIISNLYWG+ENIEDA+Q S+SE RA+ L TAEQMLQVPALLD
Sbjct: 61   ISKLRNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSSSEVRATKLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            EHGETSGM+N YLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+ 
Sbjct: 121  EHGETSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYS 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSFATCSRQEN  SMRSDS VEFGEGD+GES VRQVARKYKDWLMYYQVMSYGET+QW
Sbjct: 181  LLLSFATCSRQENCTSMRSDSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETRQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGSSS+ SSEDGSHSLHGSFSR E SKA D GLA PT+ HYD I PLD   VFQDKT 
Sbjct: 241  QQQGSSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTN 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
             SQD PRCE+ G+  K LGLIPE Q +D G W DSSTK IGDLLKDSH G+PTSLFSSMN
Sbjct: 301  TSQDVPRCEELGNSGKNLGLIPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            DSESDSD EAG+NY +HSK+S + DMPE+ YQKL+YA SKSD EQ  +SL+S S+S V+E
Sbjct: 361  DSESDSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
            HY+EANM KS SN+  D+KLCS EQK+LEPQI QN  E+SE  EL +NPCKLQTFDSA+P
Sbjct: 421  HYIEANMMKSISNKFNDYKLCSEEQKDLEPQILQNCFEDSEPKELLVNPCKLQTFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            L+  QGS  QIS Q  AKGQLYH+N+ +DSKSEILGLV KAISRLCFSEG GNYD+E AV
Sbjct: 481  LALGQGSTCQISKQNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEII TS+CYN WS
Sbjct: 601  DIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            P LMLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGS
Sbjct: 661  PPLMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECR YISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEG NDIIHILMLCVNHL+ EYQLLAANLLIQLLVLDN STTS LKEEAV VLLRSV
Sbjct: 781  RIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLDNGSTTSFLKEEAVHVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILSTLGGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRDCLTTIAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRDCLTTIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL  IE FLHPGVELEERLLACLCI+NYTSGKGMQKL+NFSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTNFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQHLGA+GQIIFAITHGHGLKVIDASRT+KVLFKSKN KC+KVVQ RVY GCTDSSI
Sbjct: 1141 KEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKNLKCMKVVQGRVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIKPPSKSW+MMH KAINSLAVYKDWLF ASS+VQGSLFQNWRRH+KP+
Sbjct: 1201 QEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFCASSIVQGSLFQNWRRHEKPK 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            M I TGKGD+VQAMS+VEDFVY+I KSS ++IQIWLRK QHKVGRVSA SRITCLLTAND
Sbjct: 1261 MNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQHKVGRVSASSRITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIP+
Sbjct: 1321 MVLCGTETGKIKGWIPI 1337

BLAST of Sed0005735 vs. NCBI nr
Match: XP_004134218.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucumis sativus] >KGN57123.1 hypothetical protein Csa_010081 [Cucumis sativus])

HSP 1 Score: 2260.7 bits (5857), Expect = 0.0e+00
Identity = 1140/1339 (85.14%), Postives = 1225/1339 (91.49%), Query Frame = 0

Query: 1    MSKSCMAIA--SSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKL 60
            MSKSC+ IA  SSSS TQCP PVCENER+DLNSI+GLVVS+NQYIHEFL N EARTA+KL
Sbjct: 1    MSKSCIPIASSSSSSTTQCPLPVCENERVDLNSIRGLVVSINQYIHEFLSNAEARTAVKL 60

Query: 61   RCTAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPAL 120
            RCT+KLR QR GF EFLEQSIISNLYWGIENIEDA+Q S+SEARA+ L TAEQMLQVPAL
Sbjct: 61   RCTSKLRNQRPGFLEFLEQSIISNLYWGIENIEDAVQTSSSEARATRLQTAEQMLQVPAL 120

Query: 121  LDEHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC 180
            +DEHGETSGMEN YLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC
Sbjct: 121  VDEHGETSGMENCYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC 180

Query: 181  HDLLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQ 240
            + LLLSFATCSRQ+N  SM  +SSVEFGEGDYGES++RQVARKYKDWLMYYQVMSYGET 
Sbjct: 181  YSLLLSFATCSRQDNFRSMGFNSSVEFGEGDYGESSIRQVARKYKDWLMYYQVMSYGETH 240

Query: 241  QWDQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDK 300
            QW Q GSS++ SSEDG HSLHGSFSR E+S+ATD G   PTL HYD IPPLD   VFQDK
Sbjct: 241  QWQQLGSSNMTSSEDGPHSLHGSFSRIEASEATDCGFPRPTLSHYDIIPPLDHIDVFQDK 300

Query: 301  TRASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSS 360
             +ASQDFPRCED G+ PK+LG IPE QF++ G   DSSTKCIGD+LKDSHPG+PTSLFSS
Sbjct: 301  RKASQDFPRCEDTGNSPKELGFIPEPQFNNWGFCRDSSTKCIGDVLKDSHPGSPTSLFSS 360

Query: 361  MNDSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTV 420
            MN+SESDSD EAG+N I+H KKS + DMPE+ YQKLQY CSK D E   +SLSS S+S V
Sbjct: 361  MNNSESDSDFEAGMNDINHPKKSGQEDMPENFYQKLQYGCSKFDGEPSLISLSSASLSRV 420

Query: 421  EEHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSA 480
            +E Y +ANM KS SN+   +K  S+EQKNL+PQ+FQN+LEESE  + S+NPCKLQTFDS+
Sbjct: 421  KERYSKANMMKSISNKFNGYKSRSIEQKNLQPQVFQNFLEESEPKKKSVNPCKLQTFDSS 480

Query: 481  VPLSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEEC 540
            +P SF QGSA  I  Q  AKGQLYH+N+ RDSKSEILGLV KAISRLCFSEG GNYD+EC
Sbjct: 481  LPSSFGQGSACPILKQNSAKGQLYHANSRRDSKSEILGLVEKAISRLCFSEGLGNYDDEC 540

Query: 541  AVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLV 600
            AVEVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN V
Sbjct: 541  AVEVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSV 600

Query: 601  IEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNP 660
            IEDIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEIICTSKCYN 
Sbjct: 601  IEDIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNA 660

Query: 661  WSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSL 720
            WSPSLMLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGL+SL
Sbjct: 661  WSPSLMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLMSL 720

Query: 721  GSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISL 780
            GSILVKCMQLDGECR Y SKFI +APFL LL+SDK+EAVHI+LQVFNEIL  PRSSAISL
Sbjct: 721  GSILVKCMQLDGECRAYTSKFISVAPFLSLLESDKKEAVHIALQVFNEILHVPRSSAISL 780

Query: 781  LQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR 840
            LQR+KNEGKND+IHILMLCVNHL+ EYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR
Sbjct: 781  LQRVKNEGKNDVIHILMLCVNHLQTEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR 840

Query: 841  SVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQS 900
            SV CEE+S MQLLSASILST+GGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS +W DQS
Sbjct: 841  SVTCEESSAMQLLSASILSTIGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSINWLDQS 900

Query: 901  LQDADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSN 960
            LQDA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRDCLTTIAWLGCEIAKSP +
Sbjct: 901  LQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSDIKKVSRDCLTTIAWLGCEIAKSPDS 960

Query: 961  IKCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLS 1020
            I+CSACEILL GIE FLHPGVELEERLLACLCI+NYTSGKGMQKL+ FSEGVRESLRRLS
Sbjct: 961  IRCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTRFSEGVRESLRRLS 1020

Query: 1021 HITWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSI 1080
            HITWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSI
Sbjct: 1021 HITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSI 1080

Query: 1081 KVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVI 1140
            KVWNIKGQSASLLWDIKKHRKAVTCF+HFESGESLLSGSADKTIRVWKM+ G+LECIEVI
Sbjct: 1081 KVWNIKGQSASLLWDIKKHRKAVTCFAHFESGESLLSGSADKTIRVWKMIHGRLECIEVI 1140

Query: 1141 ESKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDS 1200
            ESKEQIQHLGA+GQIIFA+THG+GLKVIDASRT+KVLFKSKN KCIKVVQARVY GCTDS
Sbjct: 1141 ESKEQIQHLGAYGQIIFAVTHGYGLKVIDASRTTKVLFKSKNLKCIKVVQARVYAGCTDS 1200

Query: 1201 SIQEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDK 1260
            SIQEFSV+NKWEQEIKPPSKSWI+MHQKAINSLAVYKDWLFSASSMVQGSL QNWRRH+K
Sbjct: 1201 SIQEFSVTNKWEQEIKPPSKSWILMHQKAINSLAVYKDWLFSASSMVQGSLLQNWRRHEK 1260

Query: 1261 PEMTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTA 1320
            PEM I TGKGD+VQAMS+VEDFVY+I KSSAN+IQIWLRK QHKVGR SAGS+ITCLLTA
Sbjct: 1261 PEMNIITGKGDVVQAMSVVEDFVYIICKSSANSIQIWLRKAQHKVGRASAGSKITCLLTA 1320

Query: 1321 NDMVLCGTETGKIKGWIPL 1338
            NDMVLCGTETGKIKGWIPL
Sbjct: 1321 NDMVLCGTETGKIKGWIPL 1339

BLAST of Sed0005735 vs. NCBI nr
Match: KAG7018606.1 (putative E3 ubiquitin-protein ligase LIN-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2251.9 bits (5834), Expect = 0.0e+00
Identity = 1136/1337 (84.97%), Postives = 1214/1337 (90.80%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MS SCMA   SSSPTQCPP VCEN RLDLNSI+GLVVS+NQYIHEFL N EARTA+KLRC
Sbjct: 1    MSISCMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
             +KLR  +H +FEFLEQSIISNLYWG+ENIEDA+Q S SE RA+ L TAEQMLQVPALLD
Sbjct: 61   ISKLRNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATKLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            EHGETSGM+N YLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+ 
Sbjct: 121  EHGETSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYS 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSFATCSRQEN  SMRS+S VEFGEGD+GES VRQVARKYKDWLMYYQVMSYGET QW
Sbjct: 181  LLLSFATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGSSS+ SSEDGSHSLHGSFSR E SKA D GLA PT+ HYD I PLD   VFQDKT 
Sbjct: 241  QQQGSSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTN 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
             SQD PRCE+ G+  K LGL+PE Q +D G W DSSTK IGDLLKDSH G+PTSLFSSMN
Sbjct: 301  TSQDVPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            DSESDSD EAG+NY +HSK+S + DMPE+ YQKL+YA SKSD EQ  +SL+S S+S V+E
Sbjct: 361  DSESDSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
            HY+EANM KS SN+  D+KLCS EQK+LEPQI QN LE+SE  EL +NPCKLQTFDSA+P
Sbjct: 421  HYIEANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            LS  QGS  QIS +  AKGQLYH+N+ +DSKSEILGLV KAISRLCFSEG GNYD+E AV
Sbjct: 481  LSLGQGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEII TS+CYN WS
Sbjct: 601  DIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            P  MLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGS
Sbjct: 661  PPFMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECR YISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEG NDIIHILMLCVNHL+ EYQLLAANLLIQLLVLDN STTS LKEEAV VLLRSV
Sbjct: 781  RIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLDNGSTTSFLKEEAVHVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILSTLGGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRD LTTIAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRDSLTTIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL  IE FLHPGVELEERLLACLCI+NYTSGKGMQ L+NFSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGMQNLTNFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQHLGA+GQIIFAITHGHGLKVIDASRT+KVLFKSKN KC+KVVQ RVY GCTDSSI
Sbjct: 1141 KEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKNLKCMKVVQGRVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIKPPSKSW+MMH KAINSLAVYKDWLF ASS+VQGSLFQNWRRH+KP+
Sbjct: 1201 QEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFCASSIVQGSLFQNWRRHEKPK 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            M I TGKGD+VQAMS+VEDFVY+I KSS ++IQIWLRK QHKVGRVSA SRITCLLTAND
Sbjct: 1261 MNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQHKVGRVSASSRITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIP+
Sbjct: 1321 MVLCGTETGKIKGWIPI 1337

BLAST of Sed0005735 vs. NCBI nr
Match: KAG6582207.1 (putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2251.5 bits (5833), Expect = 0.0e+00
Identity = 1136/1337 (84.97%), Postives = 1214/1337 (90.80%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MS SCMA   SSSPTQCPP VCEN RLDLNSI+GLVVS+NQYIHEFL N EARTA+KLRC
Sbjct: 1    MSISCMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
             +KLR  +H +FEFLEQSIISNLYWG+ENIEDA+Q S SE RA+ L TAEQMLQVPALLD
Sbjct: 61   ISKLRNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATRLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            EHGETSGM+N YLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+ 
Sbjct: 121  EHGETSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYS 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSFATCSRQEN  SMRS+S VEFGEGD+GES VRQVARKYKDWLMYYQVMSYGET QW
Sbjct: 181  LLLSFATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGSSS+ SSEDGSHSLHGSFSR E SKA D GLA PT+ HYD I PLD   VFQDKT 
Sbjct: 241  QQQGSSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTN 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
             SQD PRCE+ G+  K LGL+PE Q +D G W DSSTK IGDLLKDSH G+PTSLFSSMN
Sbjct: 301  TSQDVPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            DSESDSD EAG+NY +HSK+S + DMPE+ YQKL+YA SKSD EQ  +SL+S S+S V+E
Sbjct: 361  DSESDSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
            HY+EANM KS SN+  D+KLCS EQK+LEPQI QN LE+SE  EL +NPCKLQTFDSA+P
Sbjct: 421  HYIEANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            LS  QGS  QIS +  AKGQLYH+N+ +DSKSEILGLV KAISRLCFSEG GNYD+E AV
Sbjct: 481  LSLGQGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEII TS+CYN WS
Sbjct: 601  DIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            P  MLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGS
Sbjct: 661  PPFMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECR YISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEG NDIIHILMLCVNHL+ EYQLLAANLLIQLLVLDN STTS LKEEAV VLLRSV
Sbjct: 781  RIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLDNGSTTSFLKEEAVHVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILSTLGGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRD LTTIAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRDSLTTIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL  IE FLHPGVELEERLLACLCI+NYTSGKGMQ L+NFSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGMQNLTNFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQHLGA+GQIIFAITHGHGLKVIDASRT+KVLFKSKN KC+KVVQ RVY GCTDSSI
Sbjct: 1141 KEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKNLKCMKVVQGRVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIKPPSKSW+MMH KAINSLAVYKDWLF ASS+VQGSLFQNWRRH+KP+
Sbjct: 1201 QEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFCASSIVQGSLFQNWRRHEKPK 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            M I TGKGD+VQAMS+VEDFVY+I KSS ++IQIWLRK QHKVGRVSA SRITCLLTAND
Sbjct: 1261 MNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQHKVGRVSASSRITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIP+
Sbjct: 1321 MVLCGTETGKIKGWIPI 1337

BLAST of Sed0005735 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 8.9e-81
Identity = 351/1495 (23.48%), Postives = 647/1495 (43.28%), Query Frame = 0

Query: 32   IKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLRRQ-----RHGFFEFLEQSIISNLYWG 91
            ++ L  +V+ +I + L N E RT  K +C  +L  +     +    E+ +Q++++NL WG
Sbjct: 15   VRFLTTTVDSFIQDRLINKEQRTQHKEQCAERLAAEDGSGDKDTEVEYSDQAVLANLDWG 74

Query: 92   IENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHGETSGMENSYLVCCSYFYLSVVKK 151
            IE +E+AI     E + + L  AE+MLQV A+L+   + +G+ NSYL   ++  LS + K
Sbjct: 75   IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKIAGVPNSYLSAWAHLNLSYLWK 134

Query: 152  LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLLL----SFATCSRQENGESMRS--- 211
            L+ +      H L+  +V P     +FA E    L L    S      +E    M     
Sbjct: 135  LRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVGWYSEERHRLMMEVIP 194

Query: 212  DS-----SVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQWDQQGSSSVLSSEDG 271
            DS     + +F E  + ES V  +     + L   + + YGE+   D+          D 
Sbjct: 195  DSADLSFTADF-EQFFNESLVLTMRPHQLEKLQKLEQL-YGES--LDENTKLYAKYYNDC 254

Query: 272  SHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTRASQDFPRCEDKGSF 331
             +S   S  +             P       IP   +FG    K+      PR +D  + 
Sbjct: 255  MNSDSSSSKKAVPMLPIAEPPMTPLHELSRTIPDFVKFGPILPKSAGFSLAPRSKDVLNE 314

Query: 332  PKKLGLIPEQQFSDR-GLWGDSSTKCIGDLLKDSHPGTPTSLFSSMNDSESDSDL-EAGI 391
              +  +       ++  +WG   T     +++++   + + L +   DS+  +++   G+
Sbjct: 315  TIRENVTSSNLKEEKLSIWGAKDT-----IIEENEDDSDSELENESVDSDDKNNIFSPGM 374

Query: 392  NYIDHSKKSTRADMPESCYQK-----------------------------------LQYA 451
              + +    T+ D+  SC +                                    L+ +
Sbjct: 375  KMMKYEGVETKVDL--SCQRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSKFLRLS 434

Query: 452  CSK---SDIEQCFVSLSSTSISTVEEHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQ 511
             S+     I     S    SI  +     E  + K+   ++ D +  SM  +N    I  
Sbjct: 435  SSRIREPTISDSLTSSPDISIDNISNADNEVMVLKNIQRKN-DNQTLSMNHENENSLILN 494

Query: 512  --------------NYLEESEQMELSLNPCK----------------------------- 571
                          N L + E++ +   P K                             
Sbjct: 495  GSSLCESDDGYQSFNSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYERKAIQ 554

Query: 572  --LQTFDSAVPLSFRQGS-------------------------ARQISNQTIAKGQL--- 631
              L+T ++  P++ +  S                         A++ SN    +G     
Sbjct: 555  EWLRTGNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGSSCSP 614

Query: 632  ------------------YHSNN-----------------------GRDSKSEILGLVGK 691
                               H N                         + +   I+  +  
Sbjct: 615  SAKDIPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVNSLKP 674

Query: 692  AISRLCFSEGSGNYDEECAVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKV 751
             IS LC SE      EE  ++++ + K       +   + K  I++ LV  +S S+  +V
Sbjct: 675  YISSLCTSENLPEC-EEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASRNREV 734

Query: 752  IRASVSLLTTIISENNLVIEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSL 811
            +R S+ +L+ +I  ++ V E +         LAT LK  + EAA LIY + P   ++ + 
Sbjct: 735  LRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQLSAH 794

Query: 812  ELLPVFVEIICTSKCYNPWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVL 871
            EL+P  V++I  +K        L++ P  A++ I+E  +   D+ +  ++   + S + +
Sbjct: 795  ELIPSLVDVI-QNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVISANGI 854

Query: 872  CGLLEVARTNHLEGLVSLGSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHIS 931
              L  V     +EG  S+ S+L+ CMQ +  C+  I+  I ++P L L  S  +      
Sbjct: 855  PTL--VKYLERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSVRGTC 914

Query: 932  LQVFNEILRA-PRSSAISLLQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVL 991
            ++  +E+++   R+S   +L  IK+EG    +H  ++ +    +E+QL  A+LL+QL +L
Sbjct: 915  VEFLSELVQLNRRTSCNQILHTIKDEGAFSTMHTFLVYLQMAPMEHQLAVASLLLQLDLL 974

Query: 992  DNCSTTSLLKEEAVQVLLRSVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKV 1051
                  S+ +EEAV+ L+ ++  ++ S  Q+ +   L  L G  + +G+ YT AWLL+  
Sbjct: 975  AEPRKMSIYREEAVETLIEALWQKDFSNTQMKALDALLFLIGHISSSGKSYTEAWLLKIA 1034

Query: 1052 GLSSDHQNMIK--SYDWFDQSL------QDADMDSWCSLMARNIICIGE--PVFHALEKG 1111
            G    +  ++K       D  L      +   ++SW   +A +++C  E   +F ALE+ 
Sbjct: 1035 GFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIA-SVLCNHENGSIFKALEEC 1094

Query: 1112 LKSKEKKVSRDCLTTIAWLGCEIAKSP-SNIKCSACEILLGGIEAFLHPGVELEERLLAC 1171
            LKS   K+++ CL    WL   +   P + ++  A + LL  +   L     LEE++LA 
Sbjct: 1095 LKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLLEEVINVLQSSKNLEEKILAT 1154

Query: 1172 LCIYNYTSGKGM-QKLSNFSEGVRESLRRLSHITWMAEELHQVADYL--MPKTSRISCVH 1231
            L +  + S     + L  +++ +  +LRRL   + +A ++ +V   L  +  T   SC  
Sbjct: 1155 LALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDIMKVILNLKSVDVTELWSC-- 1214

Query: 1232 TQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFS 1291
             +V+EL  +S+G V +++Y  G +  G++DG+IKVW+ + +   ++ +  +H KAVT  S
Sbjct: 1215 KEVVELDLSSNGEVLSMVYLNGQVLSGHTDGTIKVWDARKRIPRVIQETHEHTKAVT--S 1274

Query: 1292 HFESGESLLSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKV 1335
               SG+ L SGS DKTIRVW +    ++CI+V + KE +  L A+ ++   ++ G G+KV
Sbjct: 1275 LCSSGDRLYSGSLDKTIRVWTIKSDGIKCIDVYDIKEAVHELAANDKLACYVSQGTGVKV 1334

BLAST of Sed0005735 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 2.9e-79
Identity = 352/1487 (23.67%), Postives = 642/1487 (43.17%), Query Frame = 0

Query: 32   IKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLRRQ-----RHGFFEFLEQSIISNLYWG 91
            ++ L  +++ +I + L N E RT  K +C  +L  +     +    E+ +Q++++NL WG
Sbjct: 15   VRFLTTTIDSFIQDRLINKEQRTQHKDQCAERLAAEDGNTDKETEVEYSDQAVLANLDWG 74

Query: 92   IENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHGETSGMENSYLVCCSYFYLSVVKK 151
            IE +E+AI     E + + L  AE+MLQV A+L+   +T+G+ NSYL   ++  LS + K
Sbjct: 75   IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKTAGVPNSYLSAWAHLNLSYLWK 134

Query: 152  LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLLL----SFATCSRQENGESMRS--D 211
            L+ +      H L+  +V P     +FA E   +L L    S      +E  + M     
Sbjct: 135  LRNNIKSCIYHSLEMFIVDPFFSRIDFAPELWKNLFLPHMSSIVGWYSEERHKLMMEVLP 194

Query: 212  SSVEFG----------------------------EGDYGES---NVRQVARKYKDWL--- 271
             S +F                             E  YGES   N R  A+ Y D +   
Sbjct: 195  ESTDFSYTADFDKVFNESLVFSMRPNQLEKLQKLEQLYGESLDENTRLYAKYYNDCMNPD 254

Query: 272  --------------------------MYYQVMSYGETQQWDQQGSSSVLSSEDGSH--SL 331
                                           + +G         S +   S DG +  + 
Sbjct: 255  STSSKKVVPMLPIAEPPMTPLHELSRSVPDFVKFGPILPKSSGFSMTTRRSNDGLNETTR 314

Query: 332  HGSFSRTESSKATDRGL--ALPTLF----------HYDNIPPLDRFGVFQDKTR------ 391
                S +  SK     L  A  ++           HYD     D+  +F  + +      
Sbjct: 315  ENIASNSNHSKGEQSSLWAAKESIIEEIEDDLDSEHYDASVDSDKINIFSPEPKKNIKDE 374

Query: 392  --------------------ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCI 451
                                +  + PR     S    L    E +F  R L    +   +
Sbjct: 375  DVEPKVYRSNQKNQMNSPNISPMESPRRASNYSSTNPLRRKKESKFL-RLLSNRFTGSIV 434

Query: 452  GDLLKDSHPGTPTS-LFSS------------MNDSESDS---DLEAGINYIDHSK-KSTR 511
             D    S P T +  +F+              NDS++ S   D E  +   D S  +S  
Sbjct: 435  SDHSLSSSPDTSSDHIFTGDEEVMVRNNIKRKNDSQTPSMNQDNENSLVLNDSSHCESED 494

Query: 512  ADMPESCYQKLQYACSKSDIEQCFVSLSSTSI---------------STVEEHYVEANMN 571
                 S + KL+     S   + FV   +  I                 ++E     N  
Sbjct: 495  GYQSSSSFPKLEKLTIGSKPPKDFVCPITGQIFSDPVTLETGQTYERKAIQEWLGTGNTT 554

Query: 572  KSFSNE----------SYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNP--------C 631
               + +          +Y  K   +  K   P++ Q +   +     S +P         
Sbjct: 555  CPITRQALSANILPKTNYVLKRLIVSWKEQNPELAQEFSNSNTPRGSSCSPSAKDITMVS 614

Query: 632  KLQTFDSAVPLSFRQGSARQISNQ--TIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFS 691
             +Q    +    ++    RQ +N+   ++ G    S   + +   I+  +   I+ LC S
Sbjct: 615  SIQRTTDSPSQKYKDDYIRQRNNRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCTS 674

Query: 692  EGSGNYDEECAVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLL 751
            E   +  E+  +E++ ++K       +   + K  ++  LV  +S S   +V+R S+ +L
Sbjct: 675  ENLQDC-EQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYIL 734

Query: 752  TTIISENNLVIEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVE 811
            + +I  +  V E +         LA  LK  + EAA LIY + P   ++   EL+P  ++
Sbjct: 735  SELIFSDERVGETLNSVDSDFDCLAMLLKNGLAEAALLIYQLRPVFAQLSEHELIPSLIQ 794

Query: 812  IICTSKCYNPWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVAR 871
            +I  +K  +     L + P AA++ I+E ++   D+    ++   + S + +  +  V  
Sbjct: 795  VI-QNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVISANGIPAI--VKY 854

Query: 872  TNHLEGLVSLGSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEIL 931
             +  EG   + SIL+ CMQ +  C+  I+  I ++P L L  +  +    I ++  +E++
Sbjct: 855  LDKTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLELFHAGNDSVRGICVEFLSELV 914

Query: 932  RA-PRSSAISLLQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSL 991
            R   R+S+   LQ IK+EG    +H  ++ +    +E+Q+  A+LL+QL +L      S+
Sbjct: 915  RLNRRTSSNQTLQIIKDEGAFSTMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRKMSI 974

Query: 992  LKEEAVQVLLRSVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQN 1051
             +EEAV+ L+ ++  ++ S  Q+ +   L  L G    +G+ YT A LL+  G    +  
Sbjct: 975  YREEAVETLIEALWQKDFSNNQMKALDALLFLIGHVTSSGKSYTEAGLLKIAGFDQPYNV 1034

Query: 1052 MIK------SYDWFDQSLQDAD--MDSWCSLMARNIICIGE--PVFHALEKGLKSKEKKV 1111
            ++K      S + F ++++D    M SW   +A +++C  E   +F ALE+ LKS   K+
Sbjct: 1035 LMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVA-SVLCNHENGSIFQALEECLKSNSLKM 1094

Query: 1112 SRDCLTTIAWLGCEIAKSP-SNIKCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTS 1171
            ++ CL    WL   +   P + ++  A + LL  +   L     LEE++LA L + ++ S
Sbjct: 1095 AKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKNLEEKILASLALKSFIS 1154

Query: 1172 GKGMQK-LSNFSEGVRESLRRLSHITWMAEELHQVADYL--MPKTSRISCVHTQVLELGF 1231
               + + L  +++ +   LR+L   + +A ++ +    L  +  T   SC   +V+EL  
Sbjct: 1155 DPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKALLNLNSVDVTELWSC--KEVVELDL 1214

Query: 1232 NSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESL 1291
            +S+G V +L Y  G +  G++DG+IKVW+ + +   ++ + ++H+KAVT  S   S + L
Sbjct: 1215 SSNGEVLSLHYLNGQVLSGHADGTIKVWDARKRIPRVIQETREHKKAVT--SLCSSVDKL 1274

Query: 1292 LSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSK 1335
             S S DKTIRVW +    ++CI+V + KE +  L A+ ++   +T G G+KV +     K
Sbjct: 1275 YSSSLDKTIRVWTIKPDGIKCIDVYDVKEAVYELAANAKLACYVTQGTGVKVFNWLDAPK 1334

BLAST of Sed0005735 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 6.6e-76
Identity = 346/1491 (23.21%), Postives = 641/1491 (42.99%), Query Frame = 0

Query: 32   IKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLRRQ-----RHGFFEFLEQSIISNLYWG 91
            ++ L  +V+ +I + L N E RT  K +C  +L  +     +    E+ +Q++++NL WG
Sbjct: 15   VRFLTTTVDSFIQDRLINKEQRTQHKEQCAERLAAEDGSGDKDTEVEYSDQAVLANLDWG 74

Query: 92   IENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHGETSGMENSYLVCCSYFYLSVVKK 151
            IE +E+AI     E + + L  AE+MLQV A+L+   + +G+ NSYL   ++  LS + K
Sbjct: 75   IEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKIAGVPNSYLSAWAHLNLSYLWK 134

Query: 152  LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLL------------------------ 211
            L+ +      H L+  +V P     +FA E    L                         
Sbjct: 135  LRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVGWYSEERHRLMMEVIP 194

Query: 212  ----LSFATCSRQENGESM------RSDSSVEFGEGDYGES---NVRQVARKYKDWL--- 271
                LSF     Q   ES+           ++  E  YGES   N +  A+ Y D +   
Sbjct: 195  DSADLSFTADFEQFFNESLVLTMRPHQLEKLQKLEQLYGESLDENTKLYAKYYNDCMNSD 254

Query: 272  ---------------------------------------------------------MYY 331
                                                                     +  
Sbjct: 255  SSSSKKAVPMLPIAEPPMTPLHELSRTIPDFVKFGPILPKSAGFSLAPRSKDVLNETIRE 314

Query: 332  QVMSYGETQQ----WDQQGSSSVLSSEDGSHSL-HGSFSRTESSKATDRGLALPTLFHYD 391
             V S    ++    W  + +    + +D    L + S    + +     G+    +  Y+
Sbjct: 315  NVTSSNLKEEKLSIWGAKDTIIEENEDDSDSELDNESVDSDDKNNIFSPGM---KMMKYE 374

Query: 392  NIPPLDRFGVFQDKTRASQDFPRCEDKGSFPKKLGLIPEQQFS-DRGLWGDSSTKCIGDL 451
             +         +++  +   F   +   + P      P+     D      SS++     
Sbjct: 375  GVETKVDLSCQRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSKFLRLSSSRIREPT 434

Query: 452  LKDSHPGTPTSLFSSMNDSESDSDLEAGINYIDHSK-KSTRADMPESCYQKLQYACSKSD 511
            + DS   +P     ++++++++  +   I   + S+  S   D   S        C   D
Sbjct: 435  ISDSLTSSPDISIDNISNADNEVMVRNNIKRKNDSQTPSMNQDNENSLVLNDSSHCESED 494

Query: 512  IEQCFVSLSSTSISTVEEHYVEANMNKSF----------------SNESYDFK------- 571
              Q     SS+S+  +E+  + +   K F                + ++Y+ K       
Sbjct: 495  GYQ-----SSSSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLR 554

Query: 572  ----LCSMEQKNLEPQIF--QNYL---------EESEQMELSLNPCKLQTFDSAVP---- 631
                 C + ++ L   I    NY+         E++ ++    +        S  P    
Sbjct: 555  TGNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKD 614

Query: 632  ---LSFRQGS-----------ARQISNQTIAKGQLYHSNN--GRDSKSEILGLVGKAISR 691
               LS RQ +           ARQ SN+ +         +   + +   I+  +   IS 
Sbjct: 615  IPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISS 674

Query: 692  LCFSEGSGNYDEECAVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRAS 751
            LC SE      EE  ++++ + K       +   + K  I++ LV  +S S+  +V+R S
Sbjct: 675  LCTSENLPEC-EEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASRNREVLRTS 734

Query: 752  VSLLTTIISENNLVIEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLP 811
            + +L+ +I  ++ V E +         LAT LK  + EAA LIY + P   ++ + EL+P
Sbjct: 735  IYILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQLSAHELIP 794

Query: 812  VFVEIICTSKCYNPWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLL 871
              V++I  +K        L++ P  A++ I+E  +   D+ +  ++   + S + +  L 
Sbjct: 795  SLVDVI-QNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVISANGIPTL- 854

Query: 872  EVARTNHLEGLVSLGSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVF 931
             V     +EG  S+ S+L+ CMQ +  C+  I+  I ++P L L  S  +      ++  
Sbjct: 855  -VKYLERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSVRGTCVEFL 914

Query: 932  NEILRA-PRSSAISLLQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCS 991
            +E+++   R+S   LL  IK+EG    +H  ++ +    +E+QL  A+LL+QL +L    
Sbjct: 915  SELVQLNRRTSCNQLLHTIKDEGAFSTMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPR 974

Query: 992  TTSLLKEEAVQVLLRSVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSS 1051
              S+ +EEAV+ L+ ++  ++ S  Q+ +   L  L G  + +G+ YT AWLL+  G   
Sbjct: 975  KMSIYREEAVETLIEALWQKDFSNTQMKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQ 1034

Query: 1052 DHQNMIK--SYDWFDQSL------QDADMDSWCSLMARNIICIGE--PVFHALEKGLKSK 1111
             +  ++K       D  L      +   ++SW   +A +++C  E   +F ALE+ LKS 
Sbjct: 1035 PYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIA-SVLCNHENGSIFKALEECLKSN 1094

Query: 1112 EKKVSRDCLTTIAWLGCEIAKSP-SNIKCSACEILLGGIEAFLHPGVELEERLLACLCIY 1171
              K+++ CL    WL   +   P + ++  A + LL  +   LH    LE+ +L  L +Y
Sbjct: 1095 SLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLEEVIKVLHSSKSLEDMILVTLSLY 1154

Query: 1172 NYTSGKGMQK-LSNFSEGVRESLRRLSHITWMAEELHQVADYL--MPKTSRISCVHTQVL 1231
             + S   + + L  +++ +   LR+L   + +A ++ +    L  +  T   SC   +V+
Sbjct: 1155 PFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKALLNLNSVDVTELWSC--KEVV 1214

Query: 1232 ELGFNSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFES 1291
            EL  +S+G V +L Y  G +  G  DG+ KV + + +   ++ +  +H KAVT  S   S
Sbjct: 1215 ELDLSSNGEVLSLHYLNGQVLSGLMDGTSKVCDARKRIPRVIQETHEHTKAVT--SLCSS 1274

Query: 1292 GESLLSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKVIDAS 1335
            G+ L S S DKTIRVW +    ++CI+V + KE +  L A+ ++   ++ G G+KV + S
Sbjct: 1275 GDRLYSASLDKTIRVWTIKSDGIKCIDVYDIKEAVHELAANDKLACYVSQGTGVKVFNWS 1334

BLAST of Sed0005735 vs. ExPASy Swiss-Prot
Match: P90648 (Myosin heavy chain kinase B OS=Dictyostelium discoideum OX=44689 GN=mhkB PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-10
Identity = 70/272 (25.74%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 1029 QVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQSA 1088
            +V DY   K+  + CV T          G V ++ Y    LF G SD SIKVW++K    
Sbjct: 483  RVYDY---KSQNMECVQTLK-----GHEGPVESICYNDQYLFSGSSDHSIKVWDLK--KL 542

Query: 1089 SLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIESKEQ-IQHL 1148
              ++ ++ H K V   +   + + L SGS+DKTI+VW +    LEC   +ES  + ++ L
Sbjct: 543  RCIFTLEGHDKPV--HTVLLNDKYLFSGSSDKTIKVWDLK--TLECKYTLESHARAVKTL 602

Query: 1149 GAHGQIIFAITHGHGLKVIDASRTSKVLFKSKN----PKCIKVVQARVYVGCTDSSIQEF 1208
               GQ +F+ ++   +KV D  +T +  +  K        I ++   +Y G  D +I+ +
Sbjct: 603  CISGQYLFSGSNDKTIKVWDL-KTFRCNYTLKGHTKWVTTICILGTNLYSGSYDKTIRVW 662

Query: 1209 SVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTI 1268
            ++ +           + +  H + +  + +    LF+AS     +  + W        T 
Sbjct: 663  NLKSL-------ECSATLRGHDRWVEHMVICDKLLFTAS---DDNTIKIWDLETLRCNTT 722

Query: 1269 TTGKGDIVQAMSIVEDFVYLISKSSANNIQIW 1296
              G    VQ +++ ED   +IS S   +I++W
Sbjct: 723  LEGHNATVQCLAVWEDKKCVISCSHDQSIRVW 729

BLAST of Sed0005735 vs. ExPASy Swiss-Prot
Match: Q9NWT1 (p21-activated protein kinase-interacting protein 1 OS=Homo sapiens OX=9606 GN=PAK1IP1 PE=1 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 3.2e-06
Identity = 40/151 (26.49%), Postives = 61/151 (40.40%), Query Frame = 0

Query: 1054 NSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESL 1113
            +S    C   Y    L  G  DG I +W+ K      L  IK H+  VT  S   SG+  
Sbjct: 81   HSGTITCLKFYGNRHLISGAEDGLICIWDAK--KWECLKSIKAHKGQVTFLSIHPSGKLA 140

Query: 1114 LSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLGAHG-QIIFAITHGHGLKVIDASRTS 1173
            LS   DKT+R W +++G+   I+ I+    I      G Q +  I +   +  +D +  S
Sbjct: 141  LSVGTDKTLRTWNLVEGRSAFIKNIKQNAHIVEWSPRGEQYVVIIQNKIDIYQLDTASIS 200

Query: 1174 KVLFKSKNPKCIKVVQARVYVGCTDSSIQEF 1204
              +   K    +K +   V     D  +  F
Sbjct: 201  GTITNEKRISSVKFLSESVLAVAGDEEVIRF 229

BLAST of Sed0005735 vs. ExPASy TrEMBL
Match: A0A0A0L5U3 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G159430 PE=4 SV=1)

HSP 1 Score: 2260.7 bits (5857), Expect = 0.0e+00
Identity = 1140/1339 (85.14%), Postives = 1225/1339 (91.49%), Query Frame = 0

Query: 1    MSKSCMAIA--SSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKL 60
            MSKSC+ IA  SSSS TQCP PVCENER+DLNSI+GLVVS+NQYIHEFL N EARTA+KL
Sbjct: 1    MSKSCIPIASSSSSSTTQCPLPVCENERVDLNSIRGLVVSINQYIHEFLSNAEARTAVKL 60

Query: 61   RCTAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPAL 120
            RCT+KLR QR GF EFLEQSIISNLYWGIENIEDA+Q S+SEARA+ L TAEQMLQVPAL
Sbjct: 61   RCTSKLRNQRPGFLEFLEQSIISNLYWGIENIEDAVQTSSSEARATRLQTAEQMLQVPAL 120

Query: 121  LDEHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC 180
            +DEHGETSGMEN YLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC
Sbjct: 121  VDEHGETSGMENCYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC 180

Query: 181  HDLLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQ 240
            + LLLSFATCSRQ+N  SM  +SSVEFGEGDYGES++RQVARKYKDWLMYYQVMSYGET 
Sbjct: 181  YSLLLSFATCSRQDNFRSMGFNSSVEFGEGDYGESSIRQVARKYKDWLMYYQVMSYGETH 240

Query: 241  QWDQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDK 300
            QW Q GSS++ SSEDG HSLHGSFSR E+S+ATD G   PTL HYD IPPLD   VFQDK
Sbjct: 241  QWQQLGSSNMTSSEDGPHSLHGSFSRIEASEATDCGFPRPTLSHYDIIPPLDHIDVFQDK 300

Query: 301  TRASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSS 360
             +ASQDFPRCED G+ PK+LG IPE QF++ G   DSSTKCIGD+LKDSHPG+PTSLFSS
Sbjct: 301  RKASQDFPRCEDTGNSPKELGFIPEPQFNNWGFCRDSSTKCIGDVLKDSHPGSPTSLFSS 360

Query: 361  MNDSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTV 420
            MN+SESDSD EAG+N I+H KKS + DMPE+ YQKLQY CSK D E   +SLSS S+S V
Sbjct: 361  MNNSESDSDFEAGMNDINHPKKSGQEDMPENFYQKLQYGCSKFDGEPSLISLSSASLSRV 420

Query: 421  EEHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSA 480
            +E Y +ANM KS SN+   +K  S+EQKNL+PQ+FQN+LEESE  + S+NPCKLQTFDS+
Sbjct: 421  KERYSKANMMKSISNKFNGYKSRSIEQKNLQPQVFQNFLEESEPKKKSVNPCKLQTFDSS 480

Query: 481  VPLSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEEC 540
            +P SF QGSA  I  Q  AKGQLYH+N+ RDSKSEILGLV KAISRLCFSEG GNYD+EC
Sbjct: 481  LPSSFGQGSACPILKQNSAKGQLYHANSRRDSKSEILGLVEKAISRLCFSEGLGNYDDEC 540

Query: 541  AVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLV 600
            AVEVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN V
Sbjct: 541  AVEVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSV 600

Query: 601  IEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNP 660
            IEDIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEIICTSKCYN 
Sbjct: 601  IEDIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNA 660

Query: 661  WSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSL 720
            WSPSLMLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGL+SL
Sbjct: 661  WSPSLMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLMSL 720

Query: 721  GSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISL 780
            GSILVKCMQLDGECR Y SKFI +APFL LL+SDK+EAVHI+LQVFNEIL  PRSSAISL
Sbjct: 721  GSILVKCMQLDGECRAYTSKFISVAPFLSLLESDKKEAVHIALQVFNEILHVPRSSAISL 780

Query: 781  LQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR 840
            LQR+KNEGKND+IHILMLCVNHL+ EYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR
Sbjct: 781  LQRVKNEGKNDVIHILMLCVNHLQTEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLR 840

Query: 841  SVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQS 900
            SV CEE+S MQLLSASILST+GGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS +W DQS
Sbjct: 841  SVTCEESSAMQLLSASILSTIGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSINWLDQS 900

Query: 901  LQDADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSN 960
            LQDA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRDCLTTIAWLGCEIAKSP +
Sbjct: 901  LQDAGMDSWCSLMARNIICIGEPVFHALEKGLKSDIKKVSRDCLTTIAWLGCEIAKSPDS 960

Query: 961  IKCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLS 1020
            I+CSACEILL GIE FLHPGVELEERLLACLCI+NYTSGKGMQKL+ FSEGVRESLRRLS
Sbjct: 961  IRCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTRFSEGVRESLRRLS 1020

Query: 1021 HITWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSI 1080
            HITWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSI
Sbjct: 1021 HITWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSI 1080

Query: 1081 KVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVI 1140
            KVWNIKGQSASLLWDIKKHRKAVTCF+HFESGESLLSGSADKTIRVWKM+ G+LECIEVI
Sbjct: 1081 KVWNIKGQSASLLWDIKKHRKAVTCFAHFESGESLLSGSADKTIRVWKMIHGRLECIEVI 1140

Query: 1141 ESKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDS 1200
            ESKEQIQHLGA+GQIIFA+THG+GLKVIDASRT+KVLFKSKN KCIKVVQARVY GCTDS
Sbjct: 1141 ESKEQIQHLGAYGQIIFAVTHGYGLKVIDASRTTKVLFKSKNLKCIKVVQARVYAGCTDS 1200

Query: 1201 SIQEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDK 1260
            SIQEFSV+NKWEQEIKPPSKSWI+MHQKAINSLAVYKDWLFSASSMVQGSL QNWRRH+K
Sbjct: 1201 SIQEFSVTNKWEQEIKPPSKSWILMHQKAINSLAVYKDWLFSASSMVQGSLLQNWRRHEK 1260

Query: 1261 PEMTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTA 1320
            PEM I TGKGD+VQAMS+VEDFVY+I KSSAN+IQIWLRK QHKVGR SAGS+ITCLLTA
Sbjct: 1261 PEMNIITGKGDVVQAMSVVEDFVYIICKSSANSIQIWLRKAQHKVGRASAGSKITCLLTA 1320

Query: 1321 NDMVLCGTETGKIKGWIPL 1338
            NDMVLCGTETGKIKGWIPL
Sbjct: 1321 NDMVLCGTETGKIKGWIPL 1339

BLAST of Sed0005735 vs. ExPASy TrEMBL
Match: A0A6J1GTU3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita moschata OX=3662 GN=LOC111457509 PE=4 SV=1)

HSP 1 Score: 2250.7 bits (5831), Expect = 0.0e+00
Identity = 1135/1337 (84.89%), Postives = 1214/1337 (90.80%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MS SCMA   SSSPTQCPP VCEN RLDLNSI+GLVVS+NQYIHEFL N EARTA+KLRC
Sbjct: 1    MSISCMATPFSSSPTQCPPDVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTAVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
             +KLR  +H +FEFLEQSIISNLYWG+ENIEDA+Q S SE RA+ L TAEQMLQVPALLD
Sbjct: 61   ISKLRNHKHEYFEFLEQSIISNLYWGVENIEDAVQTSNSEVRATKLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            EHGETSGM+N YLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+ 
Sbjct: 121  EHGETSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYS 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSFATCSRQEN  SMRS+S VEFGEGD+GES VRQVARKYKDWLMYYQVMSYGET QW
Sbjct: 181  LLLSFATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGSSS+ SSEDGSHSLHGSFSR E SKA D GLA PT+ HYD I PLD   VFQDKT 
Sbjct: 241  QQQGSSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAQPTVSHYDIISPLDHIDVFQDKTN 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
             SQD PRCE+ G+  K LGL+PE Q +D G W DSSTK IGDLLKDSH G+PTSLFSSMN
Sbjct: 301  TSQDVPRCEELGNSEKNLGLVPEPQLNDGGFWRDSSTKFIGDLLKDSHLGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            DSESDSD EAG+NY +HSK+S + DMPE+ YQKL+YA SKSD EQ  +SL+S S+S V+E
Sbjct: 361  DSESDSDFEAGMNYTNHSKRSAQEDMPENFYQKLRYARSKSDTEQSLISLTSASLSRVQE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
            HY+EANM KS SN+  D+KLCS EQK+LEPQI QN LE+SE  EL +NPCKLQTFDSA+P
Sbjct: 421  HYIEANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELLVNPCKLQTFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            LS  QGS  QIS +  AKGQLYH+N+ +DSKSEILGLV KAISRLCFSEG GNYD+E AV
Sbjct: 481  LSLGQGSTCQISKKNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEII TS+CYN WS
Sbjct: 601  DIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            P  MLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGS
Sbjct: 661  PPFMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECR YISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEG NDIIHILMLCVNHL+ EYQLLAANLLIQLLVLDN STTS LKEEAV VLLRSV
Sbjct: 781  RIKNEGGNDIIHILMLCVNHLQTEYQLLAANLLIQLLVLDNGSTTSFLKEEAVHVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILSTLGGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCS+MARNIICIGEPVFHALEKGLKS  KKVSRD LTTIAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSVMARNIICIGEPVFHALEKGLKSNIKKVSRDSLTTIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL  IE FLHPGVELEERLLACLCI+NYTSGKGMQ L+NFSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGMQNLTNFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQHLGA+GQIIFAITHGHGLKVIDASRT+KVLFKSKN KC+KVVQ RVY GCTDSSI
Sbjct: 1141 KEQIQHLGAYGQIIFAITHGHGLKVIDASRTTKVLFKSKNLKCMKVVQGRVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIKPPSKSW+MMH KAINSLAVYKDWLF ASS+VQGSLFQNWRRH+KP+
Sbjct: 1201 QEFSVTNKWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFCASSIVQGSLFQNWRRHEKPK 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            M I TGKGD+VQAMS+VEDFVY+I KSS ++IQIWLRK QHKVGRVSA SRITCLLTAND
Sbjct: 1261 MNIVTGKGDVVQAMSVVEDFVYIICKSSVSSIQIWLRKAQHKVGRVSASSRITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIP+
Sbjct: 1321 MVLCGTETGKIKGWIPI 1337

BLAST of Sed0005735 vs. ExPASy TrEMBL
Match: A0A6J1C7S7 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009156 PE=4 SV=1)

HSP 1 Score: 2247.2 bits (5822), Expect = 0.0e+00
Identity = 1138/1330 (85.56%), Postives = 1216/1330 (91.43%), Query Frame = 0

Query: 8    IASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLRRQ 67
            +A +SS TQ PP VCENERLDLNSI+ LVVS+NQYIHEFL N EAR A+ LRCT+KLR Q
Sbjct: 1    MAPASSRTQFPPSVCENERLDLNSIRALVVSINQYIHEFLSNAEARIALTLRCTSKLRNQ 60

Query: 68   RHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHGETSG 127
            RHGFFEFLEQSIISNLYWGIEN+EDA+Q S SE RA+ L TAEQMLQVPALLDEHGETSG
Sbjct: 61   RHGFFEFLEQSIISNLYWGIENVEDAVQTSISEERATRLQTAEQMLQVPALLDEHGETSG 120

Query: 128  MENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLLLSFAT 187
             EN YLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQ FC+ LLLSFAT
Sbjct: 121  TENRYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQGFCYSLLLSFAT 180

Query: 188  CSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQWDQQGSSS 247
             SRQENGE+MRSDSSVEFGEGDYGES +RQVARKYKDWLMYYQVM YGETQQW Q GS+S
Sbjct: 181  SSRQENGETMRSDSSVEFGEGDYGESTIRQVARKYKDWLMYYQVMLYGETQQWKQDGSNS 240

Query: 248  VLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTRASQDFPR 307
            +L  E+GSHS+      +E+SKATD G  LPTLFHYDNI PLDR  V QDKT+ASQDFP 
Sbjct: 241  MLPPENGSHSM------SEASKATDCGFPLPTLFHYDNIHPLDRTDVIQDKTKASQDFPW 300

Query: 308  CEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMNDSESDSD 367
            CEDKG+  KKLGLIPE QF+DRG W DSSTK I DLLKDS PG+PTSLFSSMN SESDSD
Sbjct: 301  CEDKGNSQKKLGLIPELQFNDRGSWRDSSTKYIRDLLKDSQPGSPTSLFSSMNGSESDSD 360

Query: 368  LEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEEHYVEANM 427
            +E G+NY +HSK+STRAD+PE   QKL+YAC+KSD EQ  +SLSS S+STV+E Y++ +M
Sbjct: 361  IEEGMNYTNHSKRSTRADLPEKFSQKLRYACTKSDPEQSLISLSSASLSTVQEQYIKVSM 420

Query: 428  NKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVPLSFRQGS 487
             KSFS +S DFKL S+EQKNLEPQI QN LEESE MELSL   K QTF SA+P    QGS
Sbjct: 421  TKSFSKKSSDFKLRSIEQKNLEPQILQNCLEESEPMELSLKLSKFQTFGSAIPSFLGQGS 480

Query: 488  ARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAVEVSTIYK 547
            A Q+SNQ IAK QLYHS +GRDSKSEILGL+ KAISRLCFSEG GNYDEE AVEVSTIYK
Sbjct: 481  ASQVSNQNIAKAQLYHSISGRDSKSEILGLIEKAISRLCFSEGLGNYDEEYAVEVSTIYK 540

Query: 548  MLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIEDIKKKGL 607
            MLN+KTGVQY+MLKDLIMDQL+T ISTSKEEKVIRASVSLLTTIISENN VIED+KKKGL
Sbjct: 541  MLNSKTGVQYTMLKDLIMDQLLTSISTSKEEKVIRASVSLLTTIISENNSVIEDLKKKGL 600

Query: 608  QLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWSPSLMLTP 667
            +LCDLATAL+QNVHEAA LIYLISPSPREIKSL+LLPV VEIICTSKCYN WSPSLMLTP
Sbjct: 601  KLCDLATALRQNVHEAAILIYLISPSPREIKSLQLLPVLVEIICTSKCYNAWSPSLMLTP 660

Query: 668  PAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGSILVKCMQ 727
            PAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGSILVKCMQ
Sbjct: 661  PAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGSILVKCMQ 720

Query: 728  LDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQRIKNEGK 787
            LDGECRGYISKFIP+A FLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQRI NEGK
Sbjct: 721  LDGECRGYISKFIPVAQFLCLLQSDKKEAVHIALQVFNEILRVPRSSAISLLQRINNEGK 780

Query: 788  NDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSVACEETST 847
            NDIIHILMLCVNHL+ EYQLLAAN+LIQLLVL+NCSTTSLLKEEAVQVLLRSVACEE S 
Sbjct: 781  NDIIHILMLCVNHLQTEYQLLAANVLIQLLVLENCSTTSLLKEEAVQVLLRSVACEEKSA 840

Query: 848  MQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQDADMDSW 907
            MQ LSASILS LGGTF+ TGEPYTVAWLLRKVGLSSDHQNMIKS++WFDQSLQDA +DSW
Sbjct: 841  MQSLSASILSNLGGTFSWTGEPYTVAWLLRKVGLSSDHQNMIKSFNWFDQSLQDAGVDSW 900

Query: 908  CSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIKCSACEIL 967
            CSL+ARNIICIGEPVFHAL++GLKSK KKVSRDCLTTIAWLGCEIAKSPS+ + SACEIL
Sbjct: 901  CSLIARNIICIGEPVFHALDRGLKSKIKKVSRDCLTTIAWLGCEIAKSPSSTRFSACEIL 960

Query: 968  LGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHITWMAEEL 1027
            LGGIE FLHPG+ELEERLLACLCI+NY SGKGMQKL+NFSEGVRESLRRLSHITWMAEEL
Sbjct: 961  LGGIELFLHPGIELEERLLACLCIFNYASGKGMQKLANFSEGVRESLRRLSHITWMAEEL 1020

Query: 1028 HQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKVWNIKGQS 1087
            H+VADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKVWNIKGQS
Sbjct: 1021 HRVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKVWNIKGQS 1080

Query: 1088 ASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIESKEQIQHL 1147
            ASLLWDIKKHRKAVTCFSHFESGESLLSGS DKTIRVWKM+QGKLEC EVIESKEQIQHL
Sbjct: 1081 ASLLWDIKKHRKAVTCFSHFESGESLLSGSTDKTIRVWKMIQGKLECTEVIESKEQIQHL 1140

Query: 1148 GAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSIQEFSVSN 1207
            GA+GQIIFAITHGHGLKVIDA+RT+KVLFKSKN KCIKVVQARVY GCTDSSIQEFSV+N
Sbjct: 1141 GAYGQIIFAITHGHGLKVIDATRTTKVLFKSKNLKCIKVVQARVYAGCTDSSIQEFSVTN 1200

Query: 1208 KWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTITTGK 1267
            KWEQEIKPPSKSW+MMH KAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTI TGK
Sbjct: 1201 KWEQEIKPPSKSWMMMHHKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPEMTIVTGK 1260

Query: 1268 GDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTANDMVLCGTE 1327
            GDIV AMS VEDFVYLISKSSAN+IQIWLRKTQHKVGR+SAGSRITCLLTANDMVLCGTE
Sbjct: 1261 GDIVHAMSAVEDFVYLISKSSANSIQIWLRKTQHKVGRISAGSRITCLLTANDMVLCGTE 1320

Query: 1328 TGKIKGWIPL 1338
            TGK KGWIPL
Sbjct: 1321 TGKTKGWIPL 1324

BLAST of Sed0005735 vs. ExPASy TrEMBL
Match: A0A6J1ITY3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucurbita maxima OX=3661 GN=LOC111479321 PE=4 SV=1)

HSP 1 Score: 2240.3 bits (5804), Expect = 0.0e+00
Identity = 1129/1337 (84.44%), Postives = 1214/1337 (90.80%), Query Frame = 0

Query: 1    MSKSCMAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRC 60
            MS SCMA A SSSPTQCPPPVCEN RLDLNSI+GLVVS+NQYIHEFL N EART++KLRC
Sbjct: 1    MSISCMATAFSSSPTQCPPPVCENARLDLNSIRGLVVSINQYIHEFLSNAEARTSVKLRC 60

Query: 61   TAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLD 120
             +KLR  +H +FEFLEQSIISNLYWG+ENIED +Q S+SE RA+ L TAEQMLQVPALLD
Sbjct: 61   ISKLRNHKHEYFEFLEQSIISNLYWGVENIEDTVQTSSSEVRATKLQTAEQMLQVPALLD 120

Query: 121  EHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHD 180
            EHGETSGM+N YLVCCSYFYLS+VK LQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+ 
Sbjct: 121  EHGETSGMDNRYLVCCSYFYLSLVKNLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCYS 180

Query: 181  LLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQW 240
            LLLSFATCSRQEN  SMRS+S VEFGEGD+GES VRQVARKYKDWLMYYQVMSYGET QW
Sbjct: 181  LLLSFATCSRQENCTSMRSNSCVEFGEGDFGESTVRQVARKYKDWLMYYQVMSYGETHQW 240

Query: 241  DQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKTR 300
             QQGSSS+ SSEDGSHSLHGSFSR E SKA D GLA PT+ HYD I PLD    FQDK  
Sbjct: 241  QQQGSSSMFSSEDGSHSLHGSFSRIEDSKAIDCGLAKPTVSHYDIISPLDHIDAFQDKIN 300

Query: 301  ASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSMN 360
             SQD PRCE+ G+  K LGLIPE Q +D G W DSSTK IGD+LKDSH G+PTSLFSSMN
Sbjct: 301  TSQDVPRCEELGNSEKNLGLIPEPQLNDGGFWRDSSTKFIGDMLKDSHLGSPTSLFSSMN 360

Query: 361  DSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVEE 420
            DSESDSD EAG+NY +HSK+S + D+PE+ YQKL+YA SKSD EQ  +SL+S S+S V+E
Sbjct: 361  DSESDSDFEAGMNYTNHSKRSAQEDVPENFYQKLRYARSKSDTEQSLISLTSASLSRVQE 420

Query: 421  HYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAVP 480
            HY+EANM KS SN+  D+KLCS EQK+LEPQI QN LE+SE  EL +NPCKLQTFDSA+P
Sbjct: 421  HYIEANMMKSISNKFNDYKLCSEEQKDLEPQILQNCLEDSEPKELFVNPCKLQTFDSALP 480

Query: 481  LSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECAV 540
            L+  QGS  QIS Q  AKGQLYH+N+ +DSKSEILGLV KAISRLCFSEG GNYD+E AV
Sbjct: 481  LALGQGSTCQISKQNSAKGQLYHANSRKDSKSEILGLVEKAISRLCFSEGLGNYDDEYAV 540

Query: 541  EVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVIE 600
            EVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VIE
Sbjct: 541  EVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVIE 600

Query: 601  DIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPWS 660
            DIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEII TS+CYN WS
Sbjct: 601  DIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIISTSRCYNTWS 660

Query: 661  PSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLGS 720
            P LMLTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGLVSLGS
Sbjct: 661  PPLMLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLVSLGS 720

Query: 721  ILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLLQ 780
            ILVKCMQLDGECR YISKFI +APFLCLLQSDK+EAVHI+LQVFNEILR PRSSAISLLQ
Sbjct: 721  ILVKCMQLDGECRSYISKFISVAPFLCLLQSDKKEAVHITLQVFNEILRVPRSSAISLLQ 780

Query: 781  RIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRSV 840
            RIKNEG NDIIHILMLCV+HL+ EYQLLAANLLIQLLVLDN STTS LKEEAV VLLRSV
Sbjct: 781  RIKNEGGNDIIHILMLCVDHLQTEYQLLAANLLIQLLVLDNGSTTSFLKEEAVHVLLRSV 840

Query: 841  ACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSLQ 900
            ACEETS MQLLSASILSTLGGTFA TGEPYTVAWLL+KVGLSSDHQNMIKS++W DQSLQ
Sbjct: 841  ACEETSAMQLLSASILSTLGGTFAWTGEPYTVAWLLKKVGLSSDHQNMIKSFNWLDQSLQ 900

Query: 901  DADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNIK 960
            DA MDSWCSLMARNIICIGEPVFHALEKGLKS  KKVSRDCLTTIAWLGCEIAKSPS+I+
Sbjct: 901  DAGMDSWCSLMARNIICIGEPVFHALEKGLKSNIKKVSRDCLTTIAWLGCEIAKSPSSIR 960

Query: 961  CSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSHI 1020
            CSACEILL  IE FLHPGVELEERLLACLCI+NYTSGKGMQ L+NFSEGVRESLRRLSHI
Sbjct: 961  CSACEILLSRIELFLHPGVELEERLLACLCIFNYTSGKGMQNLTNFSEGVRESLRRLSHI 1020

Query: 1021 TWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIKV 1080
            TWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIKV
Sbjct: 1021 TWMAEELHQVADYLMPNNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIKV 1080

Query: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIES 1140
            WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGS+DKTIRVWKM+QG+LECIEVIES
Sbjct: 1081 WNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSSDKTIRVWKMIQGRLECIEVIES 1140

Query: 1141 KEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSSI 1200
            KEQIQH+GA+GQIIFAITHGHGLK+IDASRT+KVLFKSKN KC+KVVQARVY GCTDSSI
Sbjct: 1141 KEQIQHVGAYGQIIFAITHGHGLKMIDASRTTKVLFKSKNLKCMKVVQARVYAGCTDSSI 1200

Query: 1201 QEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKPE 1260
            QEFSV+NKWEQEIK PSKSW+MMH KAINSLAVYKDWLF ASS+VQGSLFQNWRRH+KP+
Sbjct: 1201 QEFSVTNKWEQEIKSPSKSWMMMHHKAINSLAVYKDWLFCASSIVQGSLFQNWRRHEKPK 1260

Query: 1261 MTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAND 1320
            M I T KGD+VQA+S+VEDFVY+I KSS ++IQIWLRK Q KVGRVSA SRITCLLTAND
Sbjct: 1261 MNIVTSKGDVVQAISVVEDFVYIICKSSLSSIQIWLRKAQLKVGRVSASSRITCLLTAND 1320

Query: 1321 MVLCGTETGKIKGWIPL 1338
            MVLCGTETGKIKGWIP+
Sbjct: 1321 MVLCGTETGKIKGWIPI 1337

BLAST of Sed0005735 vs. ExPASy TrEMBL
Match: A0A1S3AXG3 (putative E3 ubiquitin-protein ligase LIN-1 OS=Cucumis melo OX=3656 GN=LOC103483854 PE=4 SV=1)

HSP 1 Score: 2234.5 bits (5789), Expect = 0.0e+00
Identity = 1132/1338 (84.60%), Postives = 1221/1338 (91.26%), Query Frame = 0

Query: 1    MSKSCMAIA-SSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLR 60
            MSKSC+ IA SSSSPTQCP PVCENERLD NSI+GLVVS+NQYIHEFL N E RTA+KLR
Sbjct: 1    MSKSCIPIASSSSSPTQCPLPVCENERLDPNSIRGLVVSINQYIHEFLSNAEVRTAVKLR 60

Query: 61   CTAKLRRQRHGFFEFLEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALL 120
            CT+KLR QR GF EFLEQSIISNLYWGIENIEDA+Q S+SEA+A+ L TAEQMLQVPAL+
Sbjct: 61   CTSKLRNQRPGFLEFLEQSIISNLYWGIENIEDAVQTSSSEAKATRLQTAEQMLQVPALV 120

Query: 121  DEHGETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCH 180
            DEHGETSGMEN YLVCCSYFYLS+VKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFC+
Sbjct: 121  DEHGETSGMENRYLVCCSYFYLSIVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCY 180

Query: 181  DLLLSFATCSRQENGESMRSDSSVEFGEGDYGESNVRQVARKYKDWLMYYQVMSYGETQQ 240
             LLLSFATCSRQEN  SM  + SVEFGEGDYGES++RQVARKYKDWLMYYQVMSYGET Q
Sbjct: 181  SLLLSFATCSRQENFRSMGFNPSVEFGEGDYGESSIRQVARKYKDWLMYYQVMSYGETHQ 240

Query: 241  WDQQGSSSVLSSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLDRFGVFQDKT 300
            W Q GSS+++SSEDGS SLHGSFSR E+S+ATD   + PTL HYD IPPLD   VFQDK 
Sbjct: 241  WQQLGSSNMMSSEDGSLSLHGSFSRIETSEATDCRFSRPTLSHYDIIPPLDHIDVFQDKR 300

Query: 301  RASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSSTKCIGDLLKDSHPGTPTSLFSSM 360
            +ASQ+FPRCED  + PK LG  PE QF+D G   DSSTKC+GD+LKDSHPG+PTSLFSSM
Sbjct: 301  KASQNFPRCEDTVNSPKNLGFFPEPQFNDWGFCRDSSTKCMGDVLKDSHPGSPTSLFSSM 360

Query: 361  NDSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSLSSTSISTVE 420
            N+SESDSD EAG+N I+H KKS +ADMP+  YQKLQY CSKSD EQ  +SLSS S+S V+
Sbjct: 361  NNSESDSDFEAGMNDINHPKKSGQADMPD--YQKLQYCCSKSDREQSLISLSSASLSRVK 420

Query: 421  EHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPCKLQTFDSAV 480
            E Y +ANM KS SN+   +K  S+E+ NLE Q+FQN+LEESE  ++S+N CKLQTFDS +
Sbjct: 421  ERYTKANMMKSISNKFNGYKSRSLEKNNLETQVFQNFLEESEPKDMSVNLCKLQTFDSYL 480

Query: 481  PLSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAISRLCFSEGSGNYDEECA 540
            P S  QGSA QI  Q  + GQL H+N+ RD KSEILGLV KAISRLCFSEG GNYD+ECA
Sbjct: 481  PSSLDQGSACQIRKQN-SGGQLCHANSRRDPKSEILGLVEKAISRLCFSEGLGNYDDECA 540

Query: 541  VEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNLVI 600
            VEVST+YKMLNNKTGVQY+MLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENN VI
Sbjct: 541  VEVSTVYKMLNNKTGVQYTMLKDLIMDQLVTGISTSKEEKVIRASVSLLTTIISENNSVI 600

Query: 601  EDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEIICTSKCYNPW 660
            EDIKKKGLQLCDLATALKQNVHEAA LIYLISPSPREIKSLELLPV VEIICTSKCYN W
Sbjct: 601  EDIKKKGLQLCDLATALKQNVHEAAILIYLISPSPREIKSLELLPVLVEIICTSKCYNAW 660

Query: 661  SPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLEVARTNHLEGLVSLG 720
            SPSL LTPPAASMMIIEVMVTAFD+DTNKMHLVEISSPSVLCGLLEVARTN++EGL+SLG
Sbjct: 661  SPSLTLTPPAASMMIIEVMVTAFDEDTNKMHLVEISSPSVLCGLLEVARTNNVEGLMSLG 720

Query: 721  SILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFNEILRAPRSSAISLL 780
            SILVKCMQLDGECR Y SKFI +APFLCLL+SDK+EAVHI+LQVFNEIL  PRSSAISLL
Sbjct: 721  SILVKCMQLDGECRSYTSKFISVAPFLCLLESDKKEAVHIALQVFNEILHVPRSSAISLL 780

Query: 781  QRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRS 840
            QRIKNEGKND+IHILMLCVNHL+ EYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRS
Sbjct: 781  QRIKNEGKNDVIHILMLCVNHLQTEYQLLAANLLIQLLVLDNCSTTSLLKEEAVQVLLRS 840

Query: 841  VACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSSDHQNMIKSYDWFDQSL 900
            VACEETS+MQLLSASILST+GGTF+ TGEPYTVAWLL+KVGLSSDHQNMIKS+DW DQSL
Sbjct: 841  VACEETSSMQLLSASILSTIGGTFSWTGEPYTVAWLLKKVGLSSDHQNMIKSFDWLDQSL 900

Query: 901  QDADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTTIAWLGCEIAKSPSNI 960
            QDA MDSWCSLMARNIICIGEPVF ALEKGLKS  KKVSRDCLTTIAWLGCEIAKSP++I
Sbjct: 901  QDAGMDSWCSLMARNIICIGEPVFRALEKGLKSDIKKVSRDCLTTIAWLGCEIAKSPNSI 960

Query: 961  KCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLSNFSEGVRESLRRLSH 1020
            +CSACEILL GIE FLHPGVELEERLLACLCI+NYTSGKGMQKL+ FSEGVRESLRRLSH
Sbjct: 961  RCSACEILLSGIELFLHPGVELEERLLACLCIFNYTSGKGMQKLTRFSEGVRESLRRLSH 1020

Query: 1021 ITWMAEELHQVADYLMPKTSRISCVHTQVLELGFNSSGAVCALIYYKGLLFGGYSDGSIK 1080
            ITWMAEELHQVADYLMP  SRISCVHTQVLELGFNSSGAVCALI+YKGLLFGGYSDGSIK
Sbjct: 1021 ITWMAEELHQVADYLMPHNSRISCVHTQVLELGFNSSGAVCALIFYKGLLFGGYSDGSIK 1080

Query: 1081 VWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIE 1140
            VWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKM+QG+LECIEVIE
Sbjct: 1081 VWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIRVWKMIQGRLECIEVIE 1140

Query: 1141 SKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKCIKVVQARVYVGCTDSS 1200
            SKEQIQHLGA+GQIIFA+T+GHGLKVIDASRT+KVLFKSKN KCIKVVQARVY GCTDSS
Sbjct: 1141 SKEQIQHLGAYGQIIFAVTNGHGLKVIDASRTTKVLFKSKNLKCIKVVQARVYAGCTDSS 1200

Query: 1201 IQEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASSMVQGSLFQNWRRHDKP 1260
            IQEFSV+NKWEQEIKPPSKSWI+MHQKAINSLAVYKDWLFSASSMVQGSL QNWRRH+KP
Sbjct: 1201 IQEFSVTNKWEQEIKPPSKSWILMHQKAINSLAVYKDWLFSASSMVQGSLLQNWRRHEKP 1260

Query: 1261 EMTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKVGRVSAGSRITCLLTAN 1320
            EM I TGKG++VQAMS+VEDFVY+I KS AN+IQIWLRK QHKVGRVSAGS+ITCLLTAN
Sbjct: 1261 EMKIITGKGEVVQAMSVVEDFVYIICKSLANSIQIWLRKAQHKVGRVSAGSKITCLLTAN 1320

Query: 1321 DMVLCGTETGKIKGWIPL 1338
            DMVLCGTETGKIKGWIPL
Sbjct: 1321 DMVLCGTETGKIKGWIPL 1335

BLAST of Sed0005735 vs. TAIR 10
Match: AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 1027.3 bits (2655), Expect = 1.1e-299
Identity = 613/1354 (45.27%), Postives = 842/1354 (62.19%), Query Frame = 0

Query: 6    MAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLR 65
            MA  SSS  T       EN  LD      ++VS+N YI   + + EA  ++K +C   L 
Sbjct: 1    MAYLSSSQSTFI--SCSENSSLD-----SILVSINVYILGVISDPEAWISLKQKCITLLS 60

Query: 66   -RQRHGFFEF-LEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHG 125
              + +  FEF  E S +SNLYWGI++IE +I    SE + S L  +E+MLQ+PALLDE G
Sbjct: 61   IEEDNTLFEFSSEHSALSNLYWGIDSIEASIHPECSEEKTSRLRNSERMLQMPALLDEQG 120

Query: 126  -ETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLL 185
              TSG+ N+ L+  SYFYLS+V  LQGD  Q  LHFLQS+LVSP +V T+ A E C    
Sbjct: 121  TTTSGVPNTVLISFSYFYLSIVSYLQGDSLQSTLHFLQSVLVSPEIVRTDIAPELC---- 180

Query: 186  LSFATCSRQENGESMRSDSSVEFGEGDY-GESNVRQVARKYKDWLMYYQVMSYGETQQWD 245
                               S+ F  G Y  +  +R++ARKYK    YYQVMSYGET Q  
Sbjct: 181  ------------------ESIFFTPGVYKSDEEIREIARKYKYRATYYQVMSYGETHQPP 240

Query: 246  QQGSSSVL------SSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLD----R 305
            ++   + +        E  + + H    + E S+  ++      L  Y N+  +D     
Sbjct: 241  RECIETPVRRQKEYGQEIFAANAHSVAEKLELSETCEK------LLQYQNLHSVDLQEEE 300

Query: 306  FGVFQDKTRASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSS-TKCIGDLLKDSHPG 365
                 +K +AS+   + E+     + L    ++ ++     G S+  +C+ + L +S P 
Sbjct: 301  LNDIFNKIKASRKIEKSENNFEGSQCLDCNLQEDYNAEP--GKSTRVRCLNEFLNESQPD 360

Query: 366  TPTSLFSSMNDSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSL 425
            T   +          +D  A I  +                Q+     +K+  E    + 
Sbjct: 361  TREDI---------GTDTLANIFCVSQ--------------QQAHKEANKAYNEDTLANR 420

Query: 426  SSTSISTVEEHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPC 485
            SS+ I          N N+S       F++ + + K      +  +LE++  +   L+  
Sbjct: 421  SSSFI---------GNFNRSI------FEIQAQQSKT----TWNTHLEDASSLR-QLDLE 480

Query: 486  KLQTFDSAVPLSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAIS-RLCFSE 545
            ++  F     +SF +G  R +  QT  +G      NG ++ S     +   ++ +    E
Sbjct: 481  EISVFGQKGSISF-EGMRRNL--QTKKRG------NGLETLSRRAPTMDLWMNLQSLIKE 540

Query: 546  GSGNYDEECAVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLT 605
              GN DE+   EV+ IY+MLN K G +YSMLKD+I+DQL T IS+S+E+ VI+AS++ LT
Sbjct: 541  VLGNADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALT 600

Query: 606  TIISENNLVIEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEI 665
             IIS N   +E++K+KGL L  LA ALKQNV EAA LIYLI PSP EIKSLELLP  V++
Sbjct: 601  KIISVNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSPTEIKSLELLPALVDV 660

Query: 666  IC----TSKCYNPWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLE 725
            +     +S CY        LTPPAAS+MIIEV++TAFD  TN MHL  ISSPSVLCGLL+
Sbjct: 661  VASTSSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMHLAAISSPSVLCGLLD 720

Query: 726  VARTNHLEGLVSLGSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFN 785
            VA++ +    +SL SILVKCMQ DG  R YI +   +APF  LLQS  +E + I+LQ  +
Sbjct: 721  VAKSGNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLH 780

Query: 786  EILRAPRSSAISLLQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTT 845
            E+L+ PRSSAI +LQ+IK EG  DI   L+ C+ HL+ +++L AA++L+QL  LD+    
Sbjct: 781  EVLKIPRSSAIKILQQIKKEGSFDIKVTLLHCIKHLQGDHKLFAADILLQLNALDSPPEN 840

Query: 846  SLLKEEAVQVLLRSVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSS-D 905
               + EA + LL +V   E S MQLLS  IL+ +GGT++ TGEPYT AWL+++ GL+S  
Sbjct: 841  KKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMS 900

Query: 906  HQNMIKSYDWFDQSLQDADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTT 965
            H NMI++ +W D+ LQD  +D WC  +AR II  G+  F  L++GLKSK K VS+ CL  
Sbjct: 901  HMNMIRNINWSDECLQDTGIDGWCCKIARRIIDTGKATFCGLQEGLKSKNKSVSKACLIA 960

Query: 966  IAWLGCEIAKSPSNIKCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLS 1025
            IAWL  EI+K P+++K SACE+LL  +  FLHPG+ELEERLLAC+CIYN++SGKG+ KL 
Sbjct: 961  IAWLSIEISKGPNSLKYSACEVLLDEVAQFLHPGLELEERLLACICIYNFSSGKGIHKLV 1020

Query: 1026 NFSEGVRESLRRLSHITWMAEELHQVADYLMPKT-SRISCVHTQVLELGFNSSGAVCALI 1085
            NFSEGVRESLRRLSH+TWMA+ELH+   YL  K+  RISCVHTQ +E+  + SGAV ALI
Sbjct: 1021 NFSEGVRESLRRLSHVTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALI 1080

Query: 1086 YYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIR 1145
            Y+KGLLF G+SDGSI+VWN+  + A+LLWDIK+H+  VTCFS  E+GE +LSGSADKTIR
Sbjct: 1081 YHKGLLFSGFSDGSIRVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIR 1140

Query: 1146 VWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKC 1205
            VW++++GKLEC EVI++K+ I+ L A G +IF IT GH +K++D+SR S+ +FK K  K 
Sbjct: 1141 VWQIVKGKLECAEVIKTKDSIRKLEAFGNMIFVITKGHKMKLLDSSRISQSIFKGKGVKS 1200

Query: 1206 IKVVQARVYVGCTDSSIQEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASS 1265
            +   Q ++Y+GC D+SIQE  V+NK E+EIK P++SW  +  K INS+ VYKD L+S+S+
Sbjct: 1201 MVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSW-RLQNKPINSVVVYKDMLYSSST 1260

Query: 1266 MVQGSLFQNWRRHDKPEMTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKV 1325
             V+ S  ++ RR+ +P+M+IT  KG  + AM +VEDF+YL   SSAN +QIWLR+TQ KV
Sbjct: 1261 YVEMSNIKDLRRNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQIWLRRTQQKV 1264

Query: 1326 GRVSAGSRITCLLTANDMVLCGTETGKIKGWIPL 1338
            GR+SAGS+IT LLTAND+V CGTE G IKGWIPL
Sbjct: 1321 GRLSAGSKITSLLTANDIVFCGTEAGVIKGWIPL 1264

BLAST of Sed0005735 vs. TAIR 10
Match: AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 1011.5 bits (2614), Expect = 6.2e-295
Identity = 606/1346 (45.02%), Postives = 835/1346 (62.04%), Query Frame = 0

Query: 6    MAIASSSSPTQCPPPVCENERLDLNSIKGLVVSVNQYIHEFLCNVEARTAIKLRCTAKLR 65
            MA  SSS  T       EN  LD      ++VS+N YI   + + EA  ++K +C   L 
Sbjct: 1    MAYLSSSQSTFI--SCSENSSLD-----SILVSINVYILGVISDPEAWISLKQKCITLLS 60

Query: 66   -RQRHGFFEF-LEQSIISNLYWGIENIEDAIQASTSEARASSLHTAEQMLQVPALLDEHG 125
              + +  FEF  E S +SNLYWGI++IE +I    SE + S L  +E+MLQ+PALLDE G
Sbjct: 61   IEEDNTLFEFSSEHSALSNLYWGIDSIEASIHPECSEEKTSRLRNSERMLQMPALLDEQG 120

Query: 126  -ETSGMENSYLVCCSYFYLSVVKKLQGDEWQVALHFLQSLLVSPRLVLTEFAQEFCHDLL 185
              TSG+ N+ L+  SYFYLS+V  LQGD  Q  LHFLQS+LVSP +V T+ A E C    
Sbjct: 121  TTTSGVPNTVLISFSYFYLSIVSYLQGDSLQSTLHFLQSVLVSPEIVRTDIAPELC---- 180

Query: 186  LSFATCSRQENGESMRSDSSVEFGEGDY-GESNVRQVARKYKDWLMYYQVMSYGETQQWD 245
                               S+ F  G Y  +  +R++ARKYK    YYQVMSYGET Q  
Sbjct: 181  ------------------ESIFFTPGVYKSDEEIREIARKYKYRATYYQVMSYGETHQPP 240

Query: 246  QQGSSSVL------SSEDGSHSLHGSFSRTESSKATDRGLALPTLFHYDNIPPLD----R 305
            ++   + +        E  + + H    + E S+  ++      L  Y N+  +D     
Sbjct: 241  RECIETPVRRQKEYGQEIFAANAHSVAEKLELSETCEK------LLQYQNLHSVDLQEEE 300

Query: 306  FGVFQDKTRASQDFPRCEDKGSFPKKLGLIPEQQFSDRGLWGDSS-TKCIGDLLKDSHPG 365
                 +K +AS+   + E+     + L    ++ ++     G S+  +C+ + L +S P 
Sbjct: 301  LNDIFNKIKASRKIEKSENNFEGSQCLDCNLQEDYNAEP--GKSTRVRCLNEFLNESQPD 360

Query: 366  TPTSLFSSMNDSESDSDLEAGINYIDHSKKSTRADMPESCYQKLQYACSKSDIEQCFVSL 425
            T   +          +D  A I  +                Q+     +K+  E    + 
Sbjct: 361  TREDI---------GTDTLANIFCVSQ--------------QQAHKEANKAYNEDTLANR 420

Query: 426  SSTSISTVEEHYVEANMNKSFSNESYDFKLCSMEQKNLEPQIFQNYLEESEQMELSLNPC 485
            SS+ I          N N+S       F++ + + K      +  +LE++  +   L+  
Sbjct: 421  SSSFI---------GNFNRSI------FEIQAQQSKT----TWNTHLEDASSLR-QLDLE 480

Query: 486  KLQTFDSAVPLSFRQGSARQISNQTIAKGQLYHSNNGRDSKSEILGLVGKAIS-RLCFSE 545
            ++  F     +SF +G  R +  QT  +G      NG ++ S     +   ++ +    E
Sbjct: 481  EISVFGQKGSISF-EGMRRNL--QTKKRG------NGLETLSRRAPTMDLWMNLQSLIKE 540

Query: 546  GSGNYDEECAVEVSTIYKMLNNKTGVQYSMLKDLIMDQLVTGISTSKEEKVIRASVSLLT 605
              GN DE+   EV+ IY+MLN K G +YSMLKD+I+DQL T IS+S+E+ VI+AS++ LT
Sbjct: 541  VLGNADEKYVSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALT 600

Query: 606  TIISENNLVIEDIKKKGLQLCDLATALKQNVHEAATLIYLISPSPREIKSLELLPVFVEI 665
             IIS N   +E++K+KGL L  LA ALKQNV EAA LIYLI PSP EIKSLELLP  V++
Sbjct: 601  KIISVNRTALEEVKRKGLNLSHLANALKQNVQEAAILIYLIKPSPTEIKSLELLPALVDV 660

Query: 666  IC----TSKCYNPWSPSLMLTPPAASMMIIEVMVTAFDDDTNKMHLVEISSPSVLCGLLE 725
            +     +S CY        LTPPAAS+MIIEV++TAFD  TN MHL  ISSPSVLCGLL+
Sbjct: 661  VASTSSSSSCYTFIPSPSYLTPPAASLMIIEVLITAFDHATNTMHLAAISSPSVLCGLLD 720

Query: 726  VARTNHLEGLVSLGSILVKCMQLDGECRGYISKFIPMAPFLCLLQSDKEEAVHISLQVFN 785
            VA++ +    +SL SILVKCMQ DG  R YI +   +APF  LLQS  +E + I+LQ  +
Sbjct: 721  VAKSGNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLH 780

Query: 786  EILRAPRSSAISLLQRIKNEGKNDIIHILMLCVNHLEIEYQLLAANLLIQLLVLDNCSTT 845
            E+L+ PRSSAI +LQ+IK EG  DI   L+ C+ HL+ +++L AA++L+QL  LD+    
Sbjct: 781  EVLKIPRSSAIKILQQIKKEGSFDIKVTLLHCIKHLQGDHKLFAADILLQLNALDSPPEN 840

Query: 846  SLLKEEAVQVLLRSVACEETSTMQLLSASILSTLGGTFALTGEPYTVAWLLRKVGLSS-D 905
               + EA + LL +V   E S MQLLS  IL+ +GGT++ TGEPYT AWL+++ GL+S  
Sbjct: 841  KKYRNEATRALLDAVTYSEGSNMQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMS 900

Query: 906  HQNMIKSYDWFDQSLQDADMDSWCSLMARNIICIGEPVFHALEKGLKSKEKKVSRDCLTT 965
            H NMI++ +W D+ LQD  +D WC  +AR II  G+  F  L++GLKSK K VS+ CL  
Sbjct: 901  HMNMIRNINWSDECLQDTGIDGWCCKIARRIIDTGKATFCGLQEGLKSKNKSVSKACLIA 960

Query: 966  IAWLGCEIAKSPSNIKCSACEILLGGIEAFLHPGVELEERLLACLCIYNYTSGKGMQKLS 1025
            IAWL  EI+K P+++K SACE+LL  +  FLHPG+ELEERLLAC+CIYN++SGKG+ KL 
Sbjct: 961  IAWLSIEISKGPNSLKYSACEVLLDEVAQFLHPGLELEERLLACICIYNFSSGKGIHKLV 1020

Query: 1026 NFSEGVRESLRRLSHITWMAEELHQVADYLMPKT-SRISCVHTQVLELGFNSSGAVCALI 1085
            NFSEGVRESLRRLSH+TWMA+ELH+   YL  K+  RISCVHTQ +E+  + SGAV ALI
Sbjct: 1021 NFSEGVRESLRRLSHVTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALI 1080

Query: 1086 YYKGLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSHFESGESLLSGSADKTIR 1145
            Y+KGLLF G+SDGSI+VWN+  + A+LLWDIK+H+  VTCFS  E+GE +LSGSADKTIR
Sbjct: 1081 YHKGLLFSGFSDGSIRVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIR 1140

Query: 1146 VWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKVIDASRTSKVLFKSKNPKC 1205
            VW++++GKLEC EVI++K+ I+ L A G +IF IT GH +K++D+SR S+ +FK K  K 
Sbjct: 1141 VWQIVKGKLECAEVIKTKDSIRKLEAFGNMIFVITKGHKMKLLDSSRISQSIFKGKGVKS 1200

Query: 1206 IKVVQARVYVGCTDSSIQEFSVSNKWEQEIKPPSKSWIMMHQKAINSLAVYKDWLFSASS 1265
            +   Q ++Y+GC D+SIQE  V+NK E+EIK P++SW  +  K INS+ VYKD L+S+S+
Sbjct: 1201 MVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSW-RLQNKPINSVVVYKDMLYSSST 1256

Query: 1266 MVQGSLFQNWRRHDKPEMTITTGKGDIVQAMSIVEDFVYLISKSSANNIQIWLRKTQHKV 1325
             V+ S  ++ RR+ +P+M+IT  KG  + AM +VEDF+YL   SSAN +QIWLR+TQ KV
Sbjct: 1261 YVEMSNIKDLRRNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQIWLRRTQQKV 1256

Query: 1326 GRVSAGSRITCLLTANDMVLCGTETG 1330
            GR+SAGS+IT LLTAND+V CGTE G
Sbjct: 1321 GRLSAGSKITSLLTANDIVFCGTEAG 1256

BLAST of Sed0005735 vs. TAIR 10
Match: AT3G21540.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.8e-07
Identity = 48/169 (28.40%), Postives = 74/169 (43.79%), Query Frame = 0

Query: 1050 ELGFNS-SGAVCALIYYK--GLLFGGYSDGSIKVWNIKGQSASLLWDIKKHRKAVTCFSH 1109
            E+ FNS  GAV AL Y K   +L  G  D  I +W++ G+S   L+ ++ HR  VT    
Sbjct: 99   EVNFNSHKGAVTALRYNKVGSMLASGSKDNDIILWDVVGESG--LFRLRGHRDQVTDLVF 158

Query: 1110 FESGESLLSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLGAHGQIIFAITHGHGLKVI 1169
             + G+ L+S S DK +RVW +      C++++         G H ++    T      V+
Sbjct: 159  LDGGKKLVSSSKDKFLRVWDL--ETQHCMQIVS--------GHHSEVWSVDTDPEERYVV 218

Query: 1170 DASRTSKVLF-----KSKNPKCIKVVQARVYVGCTDSSIQEFSVSNKWE 1211
              S   ++ F      S N   +    A         + +E S  NKWE
Sbjct: 219  TGSADQELRFYAVKEYSSNGSLVSDSNANEI-----KASEEHSTENKWE 250

BLAST of Sed0005735 vs. TAIR 10
Match: AT5G49200.1 (WD-40 repeat family protein / zfwd4 protein (ZFWD4) )

HSP 1 Score: 55.8 bits (133), Expect = 3.0e-07
Identity = 37/96 (38.54%), Postives = 49/96 (51.04%), Query Frame = 0

Query: 1057 GAVCALIYYKGLLFGGYSDGSIKVWNIKGQSAS----LLWDIKKHRKAVTCFSHFESGES 1116
            G V A+    G+LF G S GSI VW     S S     L  ++ H   VTCF+    G+ 
Sbjct: 213  GQVNAMTIANGMLFAGTSSGSILVWKATTDSESDPFKYLTSLEGHSGEVTCFA--VGGQM 272

Query: 1117 LLSGSADKTIRVWKMMQGKLECIEVIESKEQIQHLG 1149
            L SGS DKTI++W +    L+CI  ++     QH G
Sbjct: 273  LYSGSVDKTIKMWDL--NTLQCIMTLK-----QHTG 299

BLAST of Sed0005735 vs. TAIR 10
Match: AT1G47610.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 6.7e-07
Identity = 69/273 (25.27%), Postives = 115/273 (42.12%), Query Frame = 0

Query: 1052 GFNS-SGAVCALIYYK-GLLFGGYSDGSIKVWNIKGQ---------SASLLWDIK----- 1111
            GF S SG V A++  +   +F G+ DG I+VW    +         S   L D+      
Sbjct: 57   GFKSNSGLVKAIVISREAKVFTGHQDGKIRVWKTSSKNPRVYTRAGSLPALKDVLKSSVK 116

Query: 1112 ----------------KHRKAVTCFSHFESGESLLSGSADKTIRVWKMMQGKLECIEVIE 1171
                            KH  AV+C S  E    L S S D+T++VW++    L+CIE I+
Sbjct: 117  PSNYVEVRRCRTALWIKHSDAVSCLSLAEDQGLLYSASWDRTVKVWRIHD--LKCIESIK 176

Query: 1172 SKEQ-IQHLGAHGQIIFAITHGHGLKV----IDASRTSKVLFKS--KNPKCIKVV---QA 1231
            + +  +  +     ++F  +    +KV    I   RT+  LF++  K    +  +     
Sbjct: 177  AHDDAVNSVTTAESLVFTGSADGTVKVWKREIRGKRTAHSLFQTLLKQESAVTALVTSHM 236

Query: 1232 RVYVGCTDSSIQEFSVSNKWEQEIKPPSK--SWIMMHQKAINSLAVYKDWLFSASSMVQG 1279
             VY G +D ++      N WE   K   K       H+ A+  +A     LFS ++  + 
Sbjct: 237  AVYSGSSDGAV------NFWEMGDKKLLKHCEVFKKHRLAVLCIAAAGKLLFSGAADKKI 296