Sed0005731 (gene) Chayote v1

Overview
NameSed0005731
Typegene
OrganismSechium edule (Chayote v1)
DescriptionEukaryotic aspartyl protease family protein
LocationLG07: 36075346 .. 36077471 (+)
RNA-Seq ExpressionSed0005731
SyntenySed0005731
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCATTCAAAGCATCTTTTATCATTTTTCTCTCTTCAAAATCTGTCATCTTCTTTCCCCTCTTTTCCACCCCCCAAACGCGCTTGCCGTGAAATCATAACCAATTCCTCTTCTTCCCCCTTAAATACTCCACTTCTTCCCTTCTCAGCCTCAGCCATGGCCGCCATATCCACTTCTCTCCTCCTCCTCCTCCTCACCATCCTCTCCACCTCCTTCTCCGCCGCCCTCCCCCGCCGCACCTTCCTCCACGCCCCCAAAACCACCTCCCTCCTCGACGCCGCCGCCTCCATCCAAGCCACCAGGCAGATCTTCTCACTTCAAACCCAATCTCAACTCCATCAACACGATTCCACCCCCTTCACTCTGCAACTCAACTCCAGGATTTCCCTCGCCAAATCTTCCTACCGGGACTACAAATCGCTCACCTCTTCCAGATTAAGGCGCGACTCTGCTCGAGTCACATCCTTGACCGCCAGGTTGGATCCACCCGCCGGTTTCCAGAGTCCCATCGTGTCCGGTGCGAGCCAGGGGAGCGGCGAGTATTTCTCCAGAATCGGAATCGGTAACCCTCCCAGCCCGGTTTACATGGTGCTGGATACCGGAAGTGATGTGAGCTGGGTGCAATGTGCCCCCTGCGCCGATTGCTACGAGCAAACCGATCCCATTTTCGAACCGGCTTCCTCTTCTTCCTTTACTTCTCTGTCTTGCCAAACAGAGCAATGTAAATCGCTCGATGTTTCCGAGTGCCGGAACGGAACTTGCCTCTACGAGGTCTCTTACGGCGACGGATCTTACACCGTCGGCGATTTCGTTACTGAAACGGTTACGCTTGGATCTACTTCGGTTACTGATGTTGCCCTTGGTTGCGGCCATAATAATGAAGGCTTGTTCGTCGGCGCCGCTGGTTTGCTCGGCCTCGGCGGCGGATCGTTGTCGTTTCCTTCTCAACTCAATTCCTCCTCGTTTTCTTACTGCCTCGTCGATCGCGACTCCGATTCCACCTCCACTCTCGATTTCAACTCGCCGATTTCTCCCGATGCGGTAACCGCGCCCCTCCACCGGAACCCTAATTTCGACACGTTTTTTTACCTCGGCGTCACCGGAATGAGCGTCGGCGGTGAGATTCTTCCGATTCCGGAGACGGCGTTTCGAATGAGCGAGGACGGCAACGGCGGGATTATCATCGACTCCGGCACCGCCGTGACGCGGCTGCAGACCGCCGTGTATAACTCCTTGCGCGACGCGTTTGTGAAGCGCACGCGCGAGTTGAAGGCGGCGCGTGGGGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGGCTAGAGTGGAGGTGCCGACGGTGTCGTTCCACTTCGCCGGCGGGAAGGAACTGCCGTTGCCGGCCAAGAATTACCTGATTCCGGTGGACTCCGACGGGACGTTTTGTTTTGCGTTTGCGCCTACGGATTCCACGTTGTCCATCCTCGGGAACGCCCAGCAGCAAGGGACACGTGTCGGTTTTGACCTCGCTAATTCGGTCATTGGGTTCTCTCCCAACAAATGCTAATTATTTTATTTTATTTAGTTTTATTTTTAATATTAGAAGTTTCTGTCTTGACAATTTTCTTTTTTTTATTATAGAGAGTGGATTTTCACGTGTTTCTTGGAACGGTAAGTGGCAAGTGTCGTAAATTGTGAGATGGCGTGGGGGTATTAATGGAAAGAAATTCAGATGAGCCTGGATATAACCGTTAGGAGTATTTACGAAGTCCAATTAATAATAGGTTTTAAAAAAAAAAAAAATTAAAGTAGAATTGAAATAGGAAATGGGAACCGGTCAACTGCAGGTTGCAGGTTTTCGTCGCACGTTGAACATTGGATGCTTCGGCTGTGACGTTGAGATATGTCTGTCTCTGTTCTCCAGTGTTCCAACTTTTGTCTTTTGTCTTTTCATTTTTCTCTTCATTCATAAGTTTGGTCCAAAATTATTTATTTCTGTTTTACAAATTTTTAAAACTTTTATTTTATTATTATTTTTATATATGTGAGAAGGAAATCAACCAATTCTCGAGATCATTAGTACATTATGTCGATTGAGTTATGTTTATTTGAAAACATGTGATGAAATTTGATCATCTAAAC

mRNA sequence

ATTCATTCAAAGCATCTTTTATCATTTTTCTCTCTTCAAAATCTGTCATCTTCTTTCCCCTCTTTTCCACCCCCCAAACGCGCTTGCCGTGAAATCATAACCAATTCCTCTTCTTCCCCCTTAAATACTCCACTTCTTCCCTTCTCAGCCTCAGCCATGGCCGCCATATCCACTTCTCTCCTCCTCCTCCTCCTCACCATCCTCTCCACCTCCTTCTCCGCCGCCCTCCCCCGCCGCACCTTCCTCCACGCCCCCAAAACCACCTCCCTCCTCGACGCCGCCGCCTCCATCCAAGCCACCAGGCAGATCTTCTCACTTCAAACCCAATCTCAACTCCATCAACACGATTCCACCCCCTTCACTCTGCAACTCAACTCCAGGATTTCCCTCGCCAAATCTTCCTACCGGGACTACAAATCGCTCACCTCTTCCAGATTAAGGCGCGACTCTGCTCGAGTCACATCCTTGACCGCCAGGTTGGATCCACCCGCCGGTTTCCAGAGTCCCATCGTGTCCGGTGCGAGCCAGGGGAGCGGCGAGTATTTCTCCAGAATCGGAATCGGTAACCCTCCCAGCCCGGTTTACATGGTGCTGGATACCGGAAGTGATGTGAGCTGGGTGCAATGTGCCCCCTGCGCCGATTGCTACGAGCAAACCGATCCCATTTTCGAACCGGCTTCCTCTTCTTCCTTTACTTCTCTGTCTTGCCAAACAGAGCAATGTAAATCGCTCGATGTTTCCGAGTGCCGGAACGGAACTTGCCTCTACGAGGTCTCTTACGGCGACGGATCTTACACCGTCGGCGATTTCGTTACTGAAACGGTTACGCTTGGATCTACTTCGGTTACTGATGTTGCCCTTGGTTGCGGCCATAATAATGAAGGCTTGTTCGTCGGCGCCGCTGGTTTGCTCGGCCTCGGCGGCGGATCGTTGTCGTTTCCTTCTCAACTCAATTCCTCCTCGTTTTCTTACTGCCTCGTCGATCGCGACTCCGATTCCACCTCCACTCTCGATTTCAACTCGCCGATTTCTCCCGATGCGGTAACCGCGCCCCTCCACCGGAACCCTAATTTCGACACGTTTTTTTACCTCGGCGTCACCGGAATGAGCGTCGGCGGTGAGATTCTTCCGATTCCGGAGACGGCGTTTCGAATGAGCGAGGACGGCAACGGCGGGATTATCATCGACTCCGGCACCGCCGTGACGCGGCTGCAGACCGCCGTGTATAACTCCTTGCGCGACGCGTTTGTGAAGCGCACGCGCGAGTTGAAGGCGGCGCGTGGGGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGGCTAGAGTGGAGGTGCCGACGGTGTCGTTCCACTTCGCCGGCGGGAAGGAACTGCCGTTGCCGGCCAAGAATTACCTGATTCCGGTGGACTCCGACGGGACGTTTTGTTTTGCGTTTGCGCCTACGGATTCCACGTTGTCCATCCTCGGGAACGCCCAGCAGCAAGGGACACGTGTCGGTTTTGACCTCGCTAATTCGGTCATTGGGTTCTCTCCCAACAAATGCTAATTATTTTATTTTATTTAGTTTTATTTTTAATATTAGAAGTTTCTGTCTTGACAATTTTCTTTTTTTTATTATAGAGAGTGGATTTTCACGTGTTTCTTGGAACGGTAAGTGGCAAGTGTCGTAAATTGTGAGATGGCGTGGGGGTATTAATGGAAAGAAATTCAGATGAGCCTGGATATAACCGTTAGGAGTATTTACGAAGTCCAATTAATAATAGGTTTTAAAAAAAAAAAAAATTAAAGTAGAATTGAAATAGGAAATGGGAACCGGTCAACTGCAGGTTGCAGGTTTTCGTCGCACGTTGAACATTGGATGCTTCGGCTGTGACGTTGAGATATGTCTGTCTCTGTTCTCCAGTGTTCCAACTTTTGTCTTTTGTCTTTTCATTTTTCTCTTCATTCATAAGTTTGGTCCAAAATTATTTATTTCTGTTTTACAAATTTTTAAAACTTTTATTTTATTATTATTTTTATATATGTGAGAAGGAAATCAACCAATTCTCGAGATCATTAGTACATTATGTCGATTGAGTTATGTTTATTTGAAAACATGTGATGAAATTTGATCATCTAAAC

Coding sequence (CDS)

ATGGCCGCCATATCCACTTCTCTCCTCCTCCTCCTCCTCACCATCCTCTCCACCTCCTTCTCCGCCGCCCTCCCCCGCCGCACCTTCCTCCACGCCCCCAAAACCACCTCCCTCCTCGACGCCGCCGCCTCCATCCAAGCCACCAGGCAGATCTTCTCACTTCAAACCCAATCTCAACTCCATCAACACGATTCCACCCCCTTCACTCTGCAACTCAACTCCAGGATTTCCCTCGCCAAATCTTCCTACCGGGACTACAAATCGCTCACCTCTTCCAGATTAAGGCGCGACTCTGCTCGAGTCACATCCTTGACCGCCAGGTTGGATCCACCCGCCGGTTTCCAGAGTCCCATCGTGTCCGGTGCGAGCCAGGGGAGCGGCGAGTATTTCTCCAGAATCGGAATCGGTAACCCTCCCAGCCCGGTTTACATGGTGCTGGATACCGGAAGTGATGTGAGCTGGGTGCAATGTGCCCCCTGCGCCGATTGCTACGAGCAAACCGATCCCATTTTCGAACCGGCTTCCTCTTCTTCCTTTACTTCTCTGTCTTGCCAAACAGAGCAATGTAAATCGCTCGATGTTTCCGAGTGCCGGAACGGAACTTGCCTCTACGAGGTCTCTTACGGCGACGGATCTTACACCGTCGGCGATTTCGTTACTGAAACGGTTACGCTTGGATCTACTTCGGTTACTGATGTTGCCCTTGGTTGCGGCCATAATAATGAAGGCTTGTTCGTCGGCGCCGCTGGTTTGCTCGGCCTCGGCGGCGGATCGTTGTCGTTTCCTTCTCAACTCAATTCCTCCTCGTTTTCTTACTGCCTCGTCGATCGCGACTCCGATTCCACCTCCACTCTCGATTTCAACTCGCCGATTTCTCCCGATGCGGTAACCGCGCCCCTCCACCGGAACCCTAATTTCGACACGTTTTTTTACCTCGGCGTCACCGGAATGAGCGTCGGCGGTGAGATTCTTCCGATTCCGGAGACGGCGTTTCGAATGAGCGAGGACGGCAACGGCGGGATTATCATCGACTCCGGCACCGCCGTGACGCGGCTGCAGACCGCCGTGTATAACTCCTTGCGCGACGCGTTTGTGAAGCGCACGCGCGAGTTGAAGGCGGCGCGTGGGGTGGCGTTGTTCGATACTTGTTACGACTTGTCGTCGAAGGCTAGAGTGGAGGTGCCGACGGTGTCGTTCCACTTCGCCGGCGGGAAGGAACTGCCGTTGCCGGCCAAGAATTACCTGATTCCGGTGGACTCCGACGGGACGTTTTGTTTTGCGTTTGCGCCTACGGATTCCACGTTGTCCATCCTCGGGAACGCCCAGCAGCAAGGGACACGTGTCGGTTTTGACCTCGCTAATTCGGTCATTGGGTTCTCTCCCAACAAATGCTAA

Protein sequence

MAAISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQHDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFSPNKC
Homology
BLAST of Sed0005731 vs. NCBI nr
Match: XP_022983595.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 737.6 bits (1903), Expect = 6.4e-209
Identity = 382/484 (78.93%), Postives = 417/484 (86.16%), Query Frame = 0

Query: 7   SLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQH--- 66
           + LL LL +L  SFSA L  RT  HAP+ TS+LD AASI  TRQ+F+ Q QS +      
Sbjct: 3   TFLLFLLPLLYASFSAVL-SRTLPHAPQ-TSVLDVAASIHTTRQVFAFQPQSPMRDESTV 62

Query: 67  -DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------------- 126
            DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D             
Sbjct: 63  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 122

Query: 127 -PPAG--------FQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPC 186
            P  G        F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 123 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 182

Query: 187 ADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 246
           ADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 183 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 242

Query: 247 ETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDRDSD 306
           ETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDRDS+
Sbjct: 243 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 302

Query: 307 STSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGG 366
           STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DGNGG
Sbjct: 303 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 362

Query: 367 IIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFH 426
           IIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTVSFH
Sbjct: 363 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 422

Query: 427 FAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFS 465
           FA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++GFS
Sbjct: 423 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 482

BLAST of Sed0005731 vs. NCBI nr
Match: XP_023544196.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 732.3 bits (1889), Expect = 2.7e-207
Identity = 378/484 (78.10%), Postives = 415/484 (85.74%), Query Frame = 0

Query: 4   ISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLH-Q 63
           ++T+  L LL +L  SFS  L  RT  HA   TS+LD AASI  TRQ+F+ Q QS +  +
Sbjct: 1   MATAFFLFLLPLLYASFSGVL-SRTLPHA-SGTSVLDVAASIHTTRQVFAFQPQSPMRDE 60

Query: 64  HDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------------- 123
            DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D             
Sbjct: 61  SDSSSLTLLLNSRVSIIKTSHTDYKSLTLSRLHRDSARVRSLTARMDLAIRGITGADLEP 120

Query: 124 ---------PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPC 183
                        F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 121 LWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 180

Query: 184 ADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 243
           ADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 181 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 240

Query: 244 ETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDRDSD 303
           ETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDRDS+
Sbjct: 241 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 300

Query: 304 STSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGG 363
           STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DGNGG
Sbjct: 301 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 360

Query: 364 IIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFH 423
           IIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTVSFH
Sbjct: 361 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 420

Query: 424 FAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFS 465
           FA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++GFS
Sbjct: 421 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 480

BLAST of Sed0005731 vs. NCBI nr
Match: KAG6601306.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032091.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731.5 bits (1887), Expect = 4.6e-207
Identity = 378/487 (77.62%), Postives = 415/487 (85.22%), Query Frame = 0

Query: 4   ISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQH 63
           ++T+  L LL +L  SFS  L  RT  HAP+ TS+LD  ASI  TRQ+F+ Q QS +   
Sbjct: 1   MATAFFLFLLPLLYASFSGVL-SRTLPHAPR-TSVLDVDASIHTTRQVFAFQPQSPMRDE 60

Query: 64  ----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD---------- 123
               DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D          
Sbjct: 61  STVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGAD 120

Query: 124 ------------PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQC 183
                           F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQC
Sbjct: 121 LEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQC 180

Query: 184 APCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 243
           APCADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGD
Sbjct: 181 APCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 240

Query: 244 FVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDR 303
           FVTETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDR
Sbjct: 241 FVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDR 300

Query: 304 DSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDG 363
           DS+STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DG
Sbjct: 301 DSESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDG 360

Query: 364 NGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTV 423
           NGGIIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTV
Sbjct: 361 NGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTV 420

Query: 424 SFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVI 465
           SFHFA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++
Sbjct: 421 SFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLV 480

BLAST of Sed0005731 vs. NCBI nr
Match: XP_022957517.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 730.3 bits (1884), Expect = 1.0e-206
Identity = 377/487 (77.41%), Postives = 415/487 (85.22%), Query Frame = 0

Query: 4   ISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQH 63
           ++T+  L LL +L  SFS  L  R+  HAP+ TS+LD  ASI  TRQ+F+ Q QS +   
Sbjct: 1   MATAFFLFLLPLLYASFSGVL-SRSLPHAPQ-TSVLDVDASIHTTRQVFAFQPQSPMRDE 60

Query: 64  ----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD---------- 123
               DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D          
Sbjct: 61  STVSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGAD 120

Query: 124 ------------PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQC 183
                           F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQC
Sbjct: 121 LEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQC 180

Query: 184 APCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 243
           APCADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGD
Sbjct: 181 APCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 240

Query: 244 FVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDR 303
           FVTETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDR
Sbjct: 241 FVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDR 300

Query: 304 DSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDG 363
           DS+STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DG
Sbjct: 301 DSESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDG 360

Query: 364 NGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTV 423
           NGGIIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTV
Sbjct: 361 NGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTV 420

Query: 424 SFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVI 465
           SFHFA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++
Sbjct: 421 SFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLV 480

BLAST of Sed0005731 vs. NCBI nr
Match: XP_004135107.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus])

HSP 1 Score: 726.1 bits (1873), Expect = 1.9e-205
Identity = 379/492 (77.03%), Postives = 417/492 (84.76%), Query Frame = 0

Query: 1   MAAISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQL 60
           MA IS  LL LL + LS     A   RT LH   TTS+LD AASIQ T+Q+F+++ +S  
Sbjct: 1   MATISLFLLSLLFSSLS-----AFHCRT-LHPTPTTSVLDVAASIQRTQQVFAVEPKSST 60

Query: 61  HQH----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------- 120
                  D +  +LQLNSRIS+ K+S+ DYKSLT SRL+RDSARV SLTAR+D       
Sbjct: 61  PDETTVSDPSSLSLQLNSRISVMKASHSDYKSLTLSRLKRDSARVRSLTARIDLAIRGIT 120

Query: 121 ----PPAG-------------FQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDV 180
                P G             F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDV
Sbjct: 121 GTDLEPLGNGGGGGSQFGTEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDV 180

Query: 181 SWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGS 240
           SWVQCAPCA+CYEQTDPIFEP SS+SFTSLSC+TEQCKSLDVSECRNGTCLYEVSYGDGS
Sbjct: 181 SWVQCAPCAECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGS 240

Query: 241 YTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSY 300
           YTVGDFVTETVTLGSTS+ ++A+GCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSY
Sbjct: 241 YTVGDFVTETVTLGSTSLGNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSY 300

Query: 301 CLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFR 360
           CLVDRDSDSTSTLDFNSPI+PDAVTAPLHRNPN DTFFYLG+TGMSVGG +LPIPET+F+
Sbjct: 301 CLVDRDSDSTSTLDFNSPITPDAVTAPLHRNPNLDTFFYLGLTGMSVGGAVLPIPETSFQ 360

Query: 361 MSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARV 420
           MSEDGNGGII+DSGTAVTRLQT VYN LRDAFVK T +L+ ARGVALFDTCYDLSSK+RV
Sbjct: 361 MSEDGNGGIIVDSGTAVTRLQTTVYNVLRDAFVKSTHDLQTARGVALFDTCYDLSSKSRV 420

Query: 421 EVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 465
           EVPTVSFHFA G ELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRVGFDL
Sbjct: 421 EVPTVSFHFANGNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 480

BLAST of Sed0005731 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 7.7e-141
Identity = 266/494 (53.85%), Postives = 341/494 (69.03%), Query Frame = 0

Query: 8   LLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQ-TQSQLHQ---- 67
           L LL +  LS   +        L  P  T++LD  +S+Q T+ I SL  T+S L      
Sbjct: 7   LSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 66

Query: 68  --------HDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARL------ 127
                   + S+P +L+L+SR +   S ++DYKSLT SRL RDS+RV  + A++      
Sbjct: 67  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 126

Query: 128 ----------DPPAGFQ-----SPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVS 187
                     +    +Q     +P+VSGASQGSGEYFSRIG+G P   +Y+VLDTGSDV+
Sbjct: 127 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVN 186

Query: 188 WVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSY 247
           W+QC PCADCY+Q+DP+F P SSS++ SL+C   QC  L+ S CR+  CLY+VSYGDGS+
Sbjct: 187 WIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSF 246

Query: 248 TVGDFVTETVTLG-STSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSY 307
           TVG+  T+TVT G S  + +VALGCGH+NEGLF GAAGLLGLGGG LS  +Q+ ++SFSY
Sbjct: 247 TVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSY 306

Query: 308 CLVDRDSDSTSTLDFNS-PISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAF 367
           CLVDRDS  +S+LDFNS  +     TAPL RN   DTF+Y+G++G SVGGE + +P+  F
Sbjct: 307 CLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIF 366

Query: 368 RMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTREL-KAARGVALFDTCYDLSSKA 427
            +   G+GG+I+D GTAVTRLQT  YNSLRDAF+K T  L K +  ++LFDTCYD SS +
Sbjct: 367 DVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 426

Query: 428 RVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGF 465
            V+VPTV+FHF GGK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQQGTR+ +
Sbjct: 427 TVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITY 486

BLAST of Sed0005731 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.0e-113
Identity = 215/464 (46.34%), Postives = 292/464 (62.93%), Query Frame = 0

Query: 16  LSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQHDSTPFTLQLNSR 75
           LS+S S + P    +       +L    ++ AT   F+    +      S+ +TL+L  R
Sbjct: 16  LSSSSSISFPDFQII------DVLQPPLTVTATLPDFN---NTHFSDESSSKYTLRLLHR 75

Query: 76  ISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLDPPA-----------GFQSPIVSGASQ 135
                 +YR++     +R+RRD+ RV+++  R+                F S IVSG  Q
Sbjct: 76  DRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQ 135

Query: 136 GSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSC 195
           GSGEYF RIG+G+PP   YMV+D+GSD+ WVQC PC  CY+Q+DP+F+PA S S+T +SC
Sbjct: 136 GSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSC 195

Query: 196 QTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGL 255
            +  C  ++ S C +G C YEV YGDGSYT G    ET+T   T V +VA+GCGH N G+
Sbjct: 196 GSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGM 255

Query: 256 FVGAAGLLGLGGGSLSFPSQLN---SSSFSYCLVDRDSDSTSTLDFNSPISP-DAVTAPL 315
           F+GAAGLLG+GGGS+SF  QL+     +F YCLV R +DST +L F     P  A   PL
Sbjct: 256 FIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPL 315

Query: 316 HRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSL 375
            RNP   +F+Y+G+ G+ VGG  +P+P+  F ++E G+GG+++D+GTAVTRL TA Y + 
Sbjct: 316 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 375

Query: 376 RDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDS 435
           RD F  +T  L  A GV++FDTCYDLS    V VPTVSF+F  G  L LPA+N+L+PVD 
Sbjct: 376 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 435

Query: 436 DGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFSPNKC 465
            GT+CFAFA + + LSI+GN QQ+G +V FD AN  +GF PN C
Sbjct: 436 SGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Sed0005731 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 7.5e-112
Identity = 221/436 (50.69%), Postives = 285/436 (65.37%), Query Frame = 0

Query: 48  TRQIFSLQTQSQLHQHDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSL--- 107
           +  +   + +S      S+  TL L+   +L+ +   D   L SSRL+RDS RV S+   
Sbjct: 52  SESLLESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATL 111

Query: 108 --------TARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQ 167
                         P GF S +VSG SQGSGEYF+R+G+G P   VYMVLDTGSD+ W+Q
Sbjct: 112 AAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQ 171

Query: 168 CAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSEC--RNGTCLYEVSYGDGSYT 227
           CAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+VSYGDGS+T
Sbjct: 172 CAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFT 231

Query: 228 VGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLN---SSSFS 287
           VGDF TET+T     V  VALGCGH+NEGLFVGAAGLLGLG G LSFP Q     +  FS
Sbjct: 232 VGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 291

Query: 288 YCLVDRDSDS--TSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILP-IPE 347
           YCLVDR + S  +S +  N+ +S  A   PL  NP  DTF+Y+G+ G+SVGG  +P +  
Sbjct: 292 YCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 351

Query: 348 TAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSS 407
           + F++ + GNGG+IIDSGT+VTRL    Y ++RDAF    + LK A   +LFDTC+DLS+
Sbjct: 352 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSN 411

Query: 408 KARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRV 465
              V+VPTV  HF G  ++ LPA NYLIPVD++G FCFAFA T   LSI+GN QQQG RV
Sbjct: 412 MNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRV 471

BLAST of Sed0005731 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 7.6e-72
Identity = 149/382 (39.01%), Postives = 212/382 (55.50%), Query Frame = 0

Query: 89  LTSSRLRRDSARVTSLTARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDT 148
           L    + R S R+  L A L+ P+G ++ + +    G GEY   + IG P  P   ++DT
Sbjct: 59  LLERAIERGSRRLQRLEAMLNGPSGVETSVYA----GDGEYLMNLSIGTPAQPFSAIMDT 118

Query: 149 GSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSY 208
           GSD+ W QC PC  C+ Q+ PIF P  SSSF++L C ++ C++L    C N  C Y   Y
Sbjct: 119 GSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGY 178

Query: 209 GDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVG-AAGLLGLGGGSLSFPSQLNS 268
           GDGS T G   TET+T GS S+ ++  GCG NN+G   G  AGL+G+G G LS PSQL+ 
Sbjct: 179 GDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV 238

Query: 269 SSFSYCLVDRDSDSTSTLDFNS---PISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEIL 328
           + FSYC+    S + S L   S    ++  +    L ++    TF+Y+ + G+SVG   L
Sbjct: 239 TKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRL 298

Query: 329 PIPETAFRM-SEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTC 388
           PI  +AF + S +G GGIIIDSGT +T      Y S+R  F+ +          + FD C
Sbjct: 299 PIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLC 358

Query: 389 YDL-SSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQ 448
           +   S  + +++PT   HF GG +L LP++NY I   S+G  C A   +   +SI GN Q
Sbjct: 359 FQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQ 418

Query: 449 QQGTRVGFDLANSVIGFSPNKC 465
           QQ   V +D  NSV+ F+  +C
Sbjct: 419 QQNMLVVYDTGNSVVSFASAQC 434

BLAST of Sed0005731 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.3e-70
Identity = 150/383 (39.16%), Postives = 212/383 (55.35%), Query Frame = 0

Query: 89  LTSSRLRRDSARVTSLTARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDT 148
           L    ++R   R+ S+ A L   +G ++P+ +    G GEY   + IG P S    ++DT
Sbjct: 60  LIKRAIKRGERRMRSINAMLQSSSGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDT 119

Query: 149 GSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSY 208
           GSD+ W QC PC  C+ Q  PIF P  SSSF++L C+++ C+ L    C N  C Y   Y
Sbjct: 120 GSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGY 179

Query: 209 GDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVG-AAGLLGLGGGSLSFPSQLNS 268
           GDGS T G   TET T  ++SV ++A GCG +N+G   G  AGL+G+G G LS PSQL  
Sbjct: 180 GDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 239

Query: 269 SSFSYCLVDRDSDSTSTLDFNSPIS--PDA--VTAPLHRNPNFDTFFYLGVTGMSVGGEI 328
             FSYC+    S S STL   S  S  P+    T  +H + N  T++Y+ + G++VGG+ 
Sbjct: 240 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLN-PTYYYITLQGITVGGDN 299

Query: 329 LPIPETAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTC 388
           L IP + F++ +DG GG+IIDSGT +T L    YN++  AF  +          +   TC
Sbjct: 300 LGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTC 359

Query: 389 YDL-SSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDST-LSILGNA 448
           +   S  + V+VP +S  F GG  L L  +N LI   ++G  C A   +    +SI GN 
Sbjct: 360 FQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNI 419

Query: 449 QQQGTRVGFDLANSVIGFSPNKC 465
           QQQ T+V +DL N  + F P +C
Sbjct: 420 QQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Sed0005731 vs. ExPASy TrEMBL
Match: A0A6J1J6A9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482126 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.1e-209
Identity = 382/484 (78.93%), Postives = 417/484 (86.16%), Query Frame = 0

Query: 7   SLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQH--- 66
           + LL LL +L  SFSA L  RT  HAP+ TS+LD AASI  TRQ+F+ Q QS +      
Sbjct: 3   TFLLFLLPLLYASFSAVL-SRTLPHAPQ-TSVLDVAASIHTTRQVFAFQPQSPMRDESTV 62

Query: 67  -DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------------- 126
            DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D             
Sbjct: 63  SDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLEP 122

Query: 127 -PPAG--------FQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPC 186
            P  G        F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQCAPC
Sbjct: 123 LPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPC 182

Query: 187 ADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 246
           ADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT
Sbjct: 183 ADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVT 242

Query: 247 ETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDRDSD 306
           ETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDRDS+
Sbjct: 243 ETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSE 302

Query: 307 STSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGG 366
           STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DGNGG
Sbjct: 303 STSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGG 362

Query: 367 IIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFH 426
           IIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTVSFH
Sbjct: 363 IIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFH 422

Query: 427 FAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFS 465
           FA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++GFS
Sbjct: 423 FADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFS 482

BLAST of Sed0005731 vs. ExPASy TrEMBL
Match: A0A6J1GZC0 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458894 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.9e-207
Identity = 377/487 (77.41%), Postives = 415/487 (85.22%), Query Frame = 0

Query: 4   ISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQH 63
           ++T+  L LL +L  SFS  L  R+  HAP+ TS+LD  ASI  TRQ+F+ Q QS +   
Sbjct: 1   MATAFFLFLLPLLYASFSGVL-SRSLPHAPQ-TSVLDVDASIHTTRQVFAFQPQSPMRDE 60

Query: 64  ----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD---------- 123
               DS+  TL LNSR+S+ K+S+ DYKSLT SRL RDSARV SLTAR+D          
Sbjct: 61  STVSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGAD 120

Query: 124 ------------PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQC 183
                           F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSWVQC
Sbjct: 121 LEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQC 180

Query: 184 APCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 243
           APCADCYEQTDPIFEP SS+SFTSLSCQT+QCKSLDVSECRNGTCLYEVSYGDGSYTVGD
Sbjct: 181 APCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGD 240

Query: 244 FVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDR 303
           FVTETVTLGSTS+T++ALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSYCLVDR
Sbjct: 241 FVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDR 300

Query: 304 DSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDG 363
           DS+STSTLDFNSPI PDAVTAPLHRNPN DTFFYLG+TGMSVGGEILPIPET+F+MS+DG
Sbjct: 301 DSESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDG 360

Query: 364 NGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTV 423
           NGGIIIDSGTAVTRLQT  YN LRDAFVK+T +L++ARGVALFDTCYDLSSK+RVEVPTV
Sbjct: 361 NGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTV 420

Query: 424 SFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVI 465
           SFHFA GKELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRV FDLANS++
Sbjct: 421 SFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLV 480

BLAST of Sed0005731 vs. ExPASy TrEMBL
Match: A0A0A0KUG1 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608450 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.3e-206
Identity = 379/492 (77.03%), Postives = 417/492 (84.76%), Query Frame = 0

Query: 1   MAAISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQL 60
           MA IS  LL LL + LS     A   RT LH   TTS+LD AASIQ T+Q+F+++ +S  
Sbjct: 1   MATISLFLLSLLFSSLS-----AFHCRT-LHPTPTTSVLDVAASIQRTQQVFAVEPKSST 60

Query: 61  HQH----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------- 120
                  D +  +LQLNSRIS+ K+S+ DYKSLT SRL+RDSARV SLTAR+D       
Sbjct: 61  PDETTVSDPSSLSLQLNSRISVMKASHSDYKSLTLSRLKRDSARVRSLTARIDLAIRGIT 120

Query: 121 ----PPAG-------------FQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDV 180
                P G             F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDV
Sbjct: 121 GTDLEPLGNGGGGGSQFGTEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDV 180

Query: 181 SWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGS 240
           SWVQCAPCA+CYEQTDPIFEP SS+SFTSLSC+TEQCKSLDVSECRNGTCLYEVSYGDGS
Sbjct: 181 SWVQCAPCAECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGS 240

Query: 241 YTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSY 300
           YTVGDFVTETVTLGSTS+ ++A+GCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSY
Sbjct: 241 YTVGDFVTETVTLGSTSLGNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSY 300

Query: 301 CLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFR 360
           CLVDRDSDSTSTLDFNSPI+PDAVTAPLHRNPN DTFFYLG+TGMSVGG +LPIPET+F+
Sbjct: 301 CLVDRDSDSTSTLDFNSPITPDAVTAPLHRNPNLDTFFYLGLTGMSVGGAVLPIPETSFQ 360

Query: 361 MSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARV 420
           MSEDGNGGII+DSGTAVTRLQT VYN LRDAFVK T +L+ ARGVALFDTCYDLSSK+RV
Sbjct: 361 MSEDGNGGIIVDSGTAVTRLQTTVYNVLRDAFVKSTHDLQTARGVALFDTCYDLSSKSRV 420

Query: 421 EVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 465
           EVPTVSFHFA G ELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRVGFDL
Sbjct: 421 EVPTVSFHFANGNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 480

BLAST of Sed0005731 vs. ExPASy TrEMBL
Match: A0A1S3BFC3 (protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC103489261 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.0e-204
Identity = 375/492 (76.22%), Postives = 415/492 (84.35%), Query Frame = 0

Query: 1   MAAISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQ----T 60
           MA IS  LL LL + LS      LP          TS+LD AASIQ T+QIF+++    T
Sbjct: 1   MATISLFLLSLLFSSLSAFLCRTLP------PTPRTSVLDVAASIQRTQQIFAMEPKSST 60

Query: 61  QSQLHQHDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------- 120
             ++   DS+  +LQLNSRIS+ K+S+ DYKSLT SRL+RDSARV SLTAR+D       
Sbjct: 61  PDEITVSDSSSLSLQLNSRISVMKTSHSDYKSLTLSRLKRDSARVKSLTARIDLAIRGIT 120

Query: 121 ----PPAG-------------FQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDV 180
                P G             F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDV
Sbjct: 121 GTDLEPLGNGDGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDV 180

Query: 181 SWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGS 240
           SWVQCAPCA+CYEQTDPIFEP SS+SFTSLSC+TEQCKSLDVSECRNGTCLYEVSYGDGS
Sbjct: 181 SWVQCAPCAECYEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGS 240

Query: 241 YTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSY 300
           YTVGDFVTETVTLGSTS+ ++A+GCGHNNEGLF+GAAGLLGLGGGSLSFPSQLN+SSFSY
Sbjct: 241 YTVGDFVTETVTLGSTSLRNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSY 300

Query: 301 CLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFR 360
           CLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPN DTFFYLG+TGMSVGG +LPIPET+F+
Sbjct: 301 CLVDRDSDSTSTLDFNSPISPDAVTAPLHRNPNLDTFFYLGLTGMSVGGTVLPIPETSFQ 360

Query: 361 MSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARV 420
           MSEDGNGGII+DSGTAVTRLQT VYN LRD+FVK T +L++ARGVALFDTCYDLSSK+ V
Sbjct: 361 MSEDGNGGIIVDSGTAVTRLQTTVYNVLRDSFVKSTHDLQSARGVALFDTCYDLSSKSSV 420

Query: 421 EVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 465
           EVPTVSFHFA G ELPLPAKNYLIPVDS+GTFCFAFAPTDSTLSILGNAQQQGTRVGFDL
Sbjct: 421 EVPTVSFHFANGNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDL 480

BLAST of Sed0005731 vs. ExPASy TrEMBL
Match: A0A6J1G1W1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449928 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.1e-201
Identity = 371/490 (75.71%), Postives = 407/490 (83.06%), Query Frame = 0

Query: 1   MAAISTSLLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQL 60
           MA I   LL+ +   +S   S +LP RT       TS+LD AASIQ T +IF+ + +S +
Sbjct: 3   MAKIWLFLLIAVFASVSGGLSRSLPHRT------KTSVLDVAASIQRTTEIFAFEVKSPV 62

Query: 61  HQH----DSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------- 120
            +     DS+   LQLNSRIS+ K+S+ DYKSLT SRL RDSARV SLTAR+D       
Sbjct: 63  EEEKSVMDSSSLALQLNSRISIVKTSHSDYKSLTLSRLSRDSARVRSLTARIDLVIRGTT 122

Query: 121 ---------------PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSW 180
                              F+SPIVSGASQGSGEYFSR+GIG PPSPVYMVLDTGSDVSW
Sbjct: 123 GADLRTFGNGGGSRFRAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSW 182

Query: 181 VQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYT 240
           VQCAPCADCYEQTDPIFEP SS+SF SLSC+TEQCKSLDVSECRNGTCLYEVSYGDGS T
Sbjct: 183 VQCAPCADCYEQTDPIFEPTSSTSFASLSCETEQCKSLDVSECRNGTCLYEVSYGDGSST 242

Query: 241 VGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCL 300
           VGDFVTETVTLGSTS++++ALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLN+SSFSYCL
Sbjct: 243 VGDFVTETVTLGSTSLSNIALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNASSFSYCL 302

Query: 301 VDRDSDSTSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMS 360
           VDRDSDS STLDFNSPI PDAV APLHRNPN DTFFYLGVTG+SVGGEILPIPET+F+MS
Sbjct: 303 VDRDSDSASTLDFNSPIPPDAVIAPLHRNPNLDTFFYLGVTGLSVGGEILPIPETSFQMS 362

Query: 361 EDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEV 420
           +DGNGGIIIDSGTAVTRLQT VYN LRDAFVK T  L++ARGVALFDTCYDLSSK+RVEV
Sbjct: 363 QDGNGGIIIDSGTAVTRLQTTVYNLLRDAFVKSTPYLQSARGVALFDTCYDLSSKSRVEV 422

Query: 421 PTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLAN 465
           PTVSFHFA GKELPLPAKNYL+PVDS+GTFCFAFAPTDS LSILGNAQQQGTRV FDL N
Sbjct: 423 PTVSFHFANGKELPLPAKNYLLPVDSEGTFCFAFAPTDSALSILGNAQQQGTRVSFDLTN 482

BLAST of Sed0005731 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 586.6 bits (1511), Expect = 1.7e-167
Identity = 302/477 (63.31%), Postives = 364/477 (76.31%), Query Frame = 0

Query: 10  LLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATR--QIFSLQTQSQLHQHDSTP 69
           +  LT  S+ FS  LP      +  TTS+L+ A SI  T+    F L  Q +     S+ 
Sbjct: 11  IFFLTSHSSVFSRILPET----STTTTSILNVADSIHRTKYTSSFRLNQQEEQTHSASSS 70

Query: 70  FTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLD------------------ 129
           F+LQL+SR+S+  + + DYKSLT +RL RD+ARV SL  RLD                  
Sbjct: 71  FSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPISTMY 130

Query: 130 --PPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPCADCYEQT 189
                  ++P++SG +QGSGEYF+R+GIG P   VYMVLDTGSDV+W+QC PCADCY QT
Sbjct: 131 TTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQT 190

Query: 190 DPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGS 249
           +PIFEP+SSSS+  LSC T QC +L+VSECRN TCLYEVSYGDGSYTVGDF TET+T+GS
Sbjct: 191 EPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGS 250

Query: 250 TSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSYCLVDRDSDSTSTLDF 309
           T V +VA+GCGH+NEGLFVGAAGLLGLGGG L+ PSQLN++SFSYCLVDRDSDS ST+DF
Sbjct: 251 TLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSASTVDF 310

Query: 310 NSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGGIIIDSGT 369
            + +SPDAV APL RN   DTF+YLG+TG+SVGGE+L IP+++F M E G+GGIIIDSGT
Sbjct: 311 GTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGT 370

Query: 370 AVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFHFAGGKEL 429
           AVTRLQT +YNSLRD+FVK T +L+ A GVA+FDTCY+LS+K  VEVPTV+FHF GGK L
Sbjct: 371 AVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKML 430

Query: 430 PLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFSPNKC 465
            LPAKNY+IPVDS GTFC AFAPT S+L+I+GN QQQGTRV FDLANS+IGFS NKC
Sbjct: 431 ALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of Sed0005731 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 501.9 bits (1291), Expect = 5.5e-142
Identity = 266/494 (53.85%), Postives = 341/494 (69.03%), Query Frame = 0

Query: 8   LLLLLLTILSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQ-TQSQLHQ---- 67
           L LL +  LS   +        L  P  T++LD  +S+Q T+ I SL  T+S L      
Sbjct: 7   LSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 66

Query: 68  --------HDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSLTARL------ 127
                   + S+P +L+L+SR +   S ++DYKSLT SRL RDS+RV  + A++      
Sbjct: 67  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 126

Query: 128 ----------DPPAGFQ-----SPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVS 187
                     +    +Q     +P+VSGASQGSGEYFSRIG+G P   +Y+VLDTGSDV+
Sbjct: 127 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVN 186

Query: 188 WVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSECRNGTCLYEVSYGDGSY 247
           W+QC PCADCY+Q+DP+F P SSS++ SL+C   QC  L+ S CR+  CLY+VSYGDGS+
Sbjct: 187 WIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSF 246

Query: 248 TVGDFVTETVTLG-STSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNSSSFSY 307
           TVG+  T+TVT G S  + +VALGCGH+NEGLF GAAGLLGLGGG LS  +Q+ ++SFSY
Sbjct: 247 TVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSY 306

Query: 308 CLVDRDSDSTSTLDFNS-PISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILPIPETAF 367
           CLVDRDS  +S+LDFNS  +     TAPL RN   DTF+Y+G++G SVGGE + +P+  F
Sbjct: 307 CLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIF 366

Query: 368 RMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTREL-KAARGVALFDTCYDLSSKA 427
            +   G+GG+I+D GTAVTRLQT  YNSLRDAF+K T  L K +  ++LFDTCYD SS +
Sbjct: 367 DVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 426

Query: 428 RVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRVGF 465
            V+VPTV+FHF GGK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQQGTR+ +
Sbjct: 427 TVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITY 486

BLAST of Sed0005731 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.8e-114
Identity = 215/464 (46.34%), Postives = 292/464 (62.93%), Query Frame = 0

Query: 16  LSTSFSAALPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQHDSTPFTLQLNSR 75
           LS+S S + P    +       +L    ++ AT   F+    +      S+ +TL+L  R
Sbjct: 16  LSSSSSISFPDFQII------DVLQPPLTVTATLPDFN---NTHFSDESSSKYTLRLLHR 75

Query: 76  ISLAKSSYRDYKSLTSSRLRRDSARVTSLTARLDPPA-----------GFQSPIVSGASQ 135
                 +YR++     +R+RRD+ RV+++  R+                F S IVSG  Q
Sbjct: 76  DRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQ 135

Query: 136 GSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSC 195
           GSGEYF RIG+G+PP   YMV+D+GSD+ WVQC PC  CY+Q+DP+F+PA S S+T +SC
Sbjct: 136 GSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSC 195

Query: 196 QTEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGL 255
            +  C  ++ S C +G C YEV YGDGSYT G    ET+T   T V +VA+GCGH N G+
Sbjct: 196 GSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGM 255

Query: 256 FVGAAGLLGLGGGSLSFPSQLN---SSSFSYCLVDRDSDSTSTLDFNSPISP-DAVTAPL 315
           F+GAAGLLG+GGGS+SF  QL+     +F YCLV R +DST +L F     P  A   PL
Sbjct: 256 FIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPL 315

Query: 316 HRNPNFDTFFYLGVTGMSVGGEILPIPETAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSL 375
            RNP   +F+Y+G+ G+ VGG  +P+P+  F ++E G+GG+++D+GTAVTRL TA Y + 
Sbjct: 316 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 375

Query: 376 RDAFVKRTRELKAARGVALFDTCYDLSSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDS 435
           RD F  +T  L  A GV++FDTCYDLS    V VPTVSF+F  G  L LPA+N+L+PVD 
Sbjct: 376 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 435

Query: 436 DGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSVIGFSPNKC 465
            GT+CFAFA + + LSI+GN QQ+G +V FD AN  +GF PN C
Sbjct: 436 SGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Sed0005731 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 405.6 bits (1041), Expect = 5.4e-113
Identity = 221/436 (50.69%), Postives = 285/436 (65.37%), Query Frame = 0

Query: 48  TRQIFSLQTQSQLHQHDSTPFTLQLNSRISLAKSSYRDYKSLTSSRLRRDSARVTSL--- 107
           +  +   + +S      S+  TL L+   +L+ +   D   L SSRL+RDS RV S+   
Sbjct: 52  SESLLESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATL 111

Query: 108 --------TARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPVYMVLDTGSDVSWVQ 167
                         P GF S +VSG SQGSGEYF+R+G+G P   VYMVLDTGSD+ W+Q
Sbjct: 112 AAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQ 171

Query: 168 CAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSLDVSEC--RNGTCLYEVSYGDGSYT 227
           CAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+VSYGDGS+T
Sbjct: 172 CAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFT 231

Query: 228 VGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLN---SSSFS 287
           VGDF TET+T     V  VALGCGH+NEGLFVGAAGLLGLG G LSFP Q     +  FS
Sbjct: 232 VGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 291

Query: 288 YCLVDRDSDS--TSTLDFNSPISPDAVTAPLHRNPNFDTFFYLGVTGMSVGGEILP-IPE 347
           YCLVDR + S  +S +  N+ +S  A   PL  NP  DTF+Y+G+ G+SVGG  +P +  
Sbjct: 292 YCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 351

Query: 348 TAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRTRELKAARGVALFDTCYDLSS 407
           + F++ + GNGG+IIDSGT+VTRL    Y ++RDAF    + LK A   +LFDTC+DLS+
Sbjct: 352 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSN 411

Query: 408 KARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAFAPTDSTLSILGNAQQQGTRV 465
              V+VPTV  HF G  ++ LPA NYLIPVD++G FCFAFA T   LSI+GN QQQG RV
Sbjct: 412 MNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRV 471

BLAST of Sed0005731 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 5.5e-110
Identity = 227/456 (49.78%), Postives = 285/456 (62.50%), Query Frame = 0

Query: 24  LPRRTFLHAPKTTSLLDAAASIQATRQIFSLQTQSQLHQ-HDSTPFTLQLNSRISLAKSS 83
           LP    L  P++ SL D + S   T     L     L    D++P  L  N R+      
Sbjct: 35  LPSSATLSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDASPADL-FNLRLQRDSLR 94

Query: 84  YRDYKSLTSSRLRRDSARVTSLTARLDPPAGFQSPIVSGASQGSGEYFSRIGIGNPPSPV 143
            +   SL +    R++ + T  TA      GF   ++SG SQGSGEYF R+G+G P + V
Sbjct: 95  VKSITSLAAVSTGRNATKRTPRTA-----GGFSGAVISGLSQGSGEYFMRLGVGTPATNV 154

Query: 144 YMVLDTGSDVSWVQCAPCADCYEQTDPIFEPASSSSFTSLSCQTEQCKSL-DVSEC---R 203
           YMVLDTGSDV W+QC+PC  CY QTD IF+P  S +F ++ C +  C+ L D SEC   R
Sbjct: 155 YMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRR 214

Query: 204 NGTCLYEVSYGDGSYTVGDFVTETVTLGSTSVTDVALGCGHNNEGLFVGAAGLLGLGGGS 263
           + TCLY+VSYGDGS+T GDF TET+T     V  V LGCGH+NEGLFVGAAGLLGLG G 
Sbjct: 215 SKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGG 274

Query: 264 LSFPSQLN---SSSFSYCLVDRDSDST-----STLDF-NSPISPDAVTAPLHRNPNFDTF 323
           LSFPSQ     +  FSYCLVDR S  +     ST+ F N+ +   +V  PL  NP  DTF
Sbjct: 275 LSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTF 334

Query: 324 FYLGVTGMSVGGEILP-IPETAFRMSEDGNGGIIIDSGTAVTRLQTAVYNSLRDAFVKRT 383
           +YL + G+SVGG  +P + E+ F++   GNGG+IIDSGT+VTRL    Y +LRDAF    
Sbjct: 335 YYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGA 394

Query: 384 RELKAARGVALFDTCYDLSSKARVEVPTVSFHFAGGKELPLPAKNYLIPVDSDGTFCFAF 443
            +LK A   +LFDTC+DLS    V+VPTV FHF GG E+ LPA NYLIPV+++G FCFAF
Sbjct: 395 TKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGG-EVSLPASNYLIPVNTEGRFCFAF 454

Query: 444 APTDSTLSILGNAQQQGTRVGFDLANSVIGFSPNKC 465
           A T  +LSI+GN QQQG RV +DL  S +GF    C
Sbjct: 455 AGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022983595.16.4e-20978.93protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima][more]
XP_023544196.12.7e-20778.10protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo][more]
KAG6601306.14.6e-20777.62Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
XP_022957517.11.0e-20677.41protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata][more]
XP_004135107.11.9e-20577.03protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9LS407.7e-14153.85Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE34.0e-11346.34Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ37.5e-11250.69Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C37.6e-7239.01Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C29.3e-7039.16Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1J6A93.1e-20978.93protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GZC04.9e-20777.41protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A0A0KUG19.3e-20677.03Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G60845... [more]
A0A1S3BFC31.0e-20476.22protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC10348926... [more]
A0A6J1G1W11.1e-20175.71protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT1G25510.11.7e-16763.31Eukaryotic aspartyl protease family protein [more]
AT3G18490.15.5e-14253.85Eukaryotic aspartyl protease family protein [more]
AT3G20015.12.8e-11446.34Eukaryotic aspartyl protease family protein [more]
AT1G01300.15.4e-11350.69Eukaryotic aspartyl protease family protein [more]
AT3G61820.15.5e-11049.78Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 310..460
e-value: 1.8E-38
score: 131.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 288..464
e-value: 2.3E-53
score: 182.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 106..287
e-value: 3.2E-52
score: 179.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 123..464
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 129..287
e-value: 8.3E-50
score: 169.5
NoneNo IPR availablePANTHERPTHR13683:SF775EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 15..464
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 15..464
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 144..155
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 129..460
score: 46.157112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0005731.1Sed0005731.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity